MD15G1157600.v1.1

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004795
Physical Location & Seq
Reverse (-)
11684386 .. 11701248
16863 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1157600.v1.1.491

Sequence Viewer

Length: 4872 bp
ATGGACGGTGCTGGCGGAAGTTCTCCACCGTCTGATCAGGGCGGTTCGTCTACTGACCGGGTCAAGTCCGGTCATGGAGCTTCGAAGCCGGAGAAAGACCCGAGGAAGGACGCCAGAAAGTATCAATTGGACCTGTGCAAGAAAGCTTTGGAGGAGAACGTAATTGTGTATCTAGGGACTGGTTGTGGGAAAACCCGCATAGCCGTCATGCTTATATACGAGATGCGCCACCTGATCCGCAAACCCCAAAAGAGCGTATGCGTGTTTCTTGCACCCACAGTGGCTCTGGTTCAACAGCAAGCTAGGGTTATAGAAGATTCCCTTGGTTTCGATGTTGGGATTTATTGTGGAGGCTCTAACCAATTGAAGAATCACCAAGACTGGGAAAAAGAGATTGAACAATATGAGGTTTTTGTTATGATCCCTGAAGTACTGCTACGAAACTTATGTCACTGTTTCATTAAGATGGAGTCAATTTCTTTGTTGATATTTGATGAGTGTCATCATGCTCAAGTCCAAAGCAACCATGCCTATGCAGAAATTATGAAATTGTTTTACCATGCTAATACCACAAAACATCCTCGTATATTTGGCATGACGGCATCTCCAGTTGTAGGGAAAGGTGCTTCGAGTCGGGAAAACTTATCAAAATGCATCAATAGTCTTGAAAGTATACTTGATGCTAAGGTTTATTCAGTTGATAAGAAAGAATTGGATTGTTTTGTCTCATCTCCTGTCATCACAGTGTATGATTATGGTCCAGTTATAAGCGACGCATCCAGCCCCTATAGCACTTACTACACTAAACTTGAGCAGATGAAACACCAGTGTATAGCAGAACTTGGTAAGAAGACAAATGATCTCCAAAGTCTAAGGAGTACAAAGAAACTGTTGAACAGAATGCATGAAAGTATAAGGTTTTGCTTGGAAAATCTTGGCCTTTGGGGAGCATTAAAAGCTAGTCATGTTCTTTTGAGTGGCGATCACTTGGAAAGGAGAGAACTAATAGAGGAGGAAGGAAATAATGGTGATGATTCTCTATGTGTTAGATATCTGACTCAGGTTGATGACATCCTTACTACTGACTGCTTAAAAGATTCCATTGCAGATGATCTATCTTCTATAGATATTTTAAAGGAGCCATTTTTCTCAAGAAAGCTCTTACGTCTTATTGGAGTTCTTTCATCCTTTAGGTTGCAACAAAATATGAAATGCATAATTTTTGTCAATAGAATTGTTACTGCAAGTTCCTTGTCGCACATACTGCAACGTCTCAAGTTTCTAGCATCTTGGAAGTGTGATTTTCTTGTAGGAGTCAACTCTAGACTGATGAATATGTCAAGGAAGAAGATGAAAATCATTCTTGATAAGTTCCGATCTGGAGAGTTGAATCTATTGGTTGCAACTAAAGTTGGTGAAGAAGGACTAGATATTCAGACATGCTGCCTTGTGATACGATTTGATCTTCCAGAAACTGTTGCTAGCTTTATACAATCACGAGGTCGTGCACGGATGCCTCAGTCTGAATATGCATTTTTGGTAAACAGTGGCAACCAGAAAGAGAGAGATTTAATAGAAACCTTCAGACAAGATGAAGATCACATGAACATGGAAATTACTTTCAGAACATCCAGTGAGACATTTGTTGGTTCGGCAGATAGGGTGTATAATGTCGATTCTTCTGGTGCTTCCATCAGTTCTGGATATAGCATCTCACTGTTACACAAGTATTGTTCAAAGCTTCCACATGATGAGTACTTTGACCCCAATCCAAAATTCTTTTTTTTCGATGACTTGGGGGGAACTGTTTGTCAGATAGTTTTACCTTCCAATGCACCAATCCATCAAATTGTTGGAACACAACAATCTTCGATGGAAGATGCCAAAAAAGATGTTTGTCTAAAAGCCATTGAAGAATTGCATAAATTAGGTGCCTTAAATGACTTTCTCTTACCACAGCAAGACAATGCAAATGAGGTGGAGTTGGTGTCTTCTGATTCTGACAGTACCGAAGATGAGGATCCAAGAGGAGAACTACATGAAATGCTAGTTCCTGCTGCACTAAAAGAGCCATGGAGTAGTTCGGAGGACCTTGTTTCCCTCAGCTCGTACTATATAAAATTTAACCCTGTTCCTGAGGACAGGATCTATAGAAGCTTTGGTCTATTTGTCAAGGCTCCTCTCCCAGTAGAAGCCGAAACAATGGAACTTGATCTTCATCTCGCTCATGGTAGATCTGTGATTACAAAGTTGGTCCCTTCAGGATTTGCCAATTTTGGCAAAGATGAGATCGTGCTGGCACAAAATTTTCAAGAAATGTTCCTCAAGCTCGTCCTTGACCGAACAGAATTTGTTTCTGATTATGTTCCATTGGGAAGGCATGATAGATCAAGCTCATCAACGTTCTACCTGTTGCTTCCAGTAATGTTGGGTAATGATGATAAGATAGCAAGCATTGATTGGGGGACTATCAGAAAATGTTTATCATCCCCAGTGTTCAGGGCTCCAGGAGATGCTACAGAGAAAGTTACTTCTTTGGATATTCGTCTTGCATCGGGTTACAAAAGCATAAGGGATATTCAGAATAGTTTAGTCTATGTCCCATATAAGAAATCATTTTACTTCATCACTGATGTTGTTCGAGAAAAGAATGGCTACAGCCAATATAAAAACTCGGGCACTTTAACCTATGTGGACCATTTAATTCAAAAATTTGGCATTTACCTTAAATACCCTGAGCAACAGCTTCTGCGTGCCAAACCACTTTTCTCTTTACACAACTTGCTACACAACCGAAGGCAAGAAGATTCGGGACCGACACAACTAGAGGAGTATTTTATTAGCTTGCCACCTGAGCTTTGCGAGTTGAAGGTAATAGGATTTTCCAAAGATATTGGGAGTTCAATATCTTTGTTACCATCACTCATGCACCGGCTGGAAAACCTGCTTGTCGCCATCGAACTGAAGCATGTATTATCTACTTCATTCCCAGAGGGAGGTGCAGTTACTGCCAAGAGAGTTCTAGAGGCACTCACAACTGAGAAGTGCCAGGAGCGGCTTTCCCTTGAAAGGCTTGAAATACTTGGTGATGCTTTCCTCAAGTTTGCTGTTGGGCGACACTTCTTCCTTCGGCATGGTAGCCTTGATGAAGGGGGACTAACAAGGAAGCGTTCTAATGTGGTAAACAATTCCAATTTGTTCAAGCTAGCCACAAGAAGCAACTTACAGGTATACATACGGGATCAACCATTTGAACCCTCTCAGTACTTTGCTTTAGGCCGTCCTTGCCCAAGAATTTGTAACAAGGAGACAGTAGGAGCTATTCATTCTCATGACGTATCTGGTGATGTAAATCACACAAGTGCTAGTGAGGTCAGATGCAGTAAGGGTCACCATTGGTTATATAATAAAACCATTGCTGATGTTGTTGAAGCCCTTGTTGGAGCATTCGTAGTTGACAGTGGCTTTATAGCTGCAACTGCATTTCTTAAGTGGATTGGCATACAAGTAGACTTCGAACCATCACAAGTTACCGAAGCATGCATAGCAAGCTCTAGATACATCCCACTTTCTGCTTGCATGGACATTGCTGCCCTTGAAAATTCATTAGGTTATAAGTTTCTCCACAGAGGTCTGCTCTTACAGGCATTTGTACATCCTTCTTACAACAAGCATGGAGGAGGCTGCTATCAGAGATTGGAGTTTCTTGGGGATGCCGTCTTGGATTACTTGATCACCTCATACCTGTATTCGGTTTATCCAAAGTTGAAGCCTGGTCAATTAACGGATTTGAGATCAGTGTCTGTGAATAATAAAGCCTTTGCTAATGTTGCAGTAGATCGGTCCTTCCACAAGTTTCTAATCAGTGATTCCTGTAGCCTTTCAGAGGCGATAGAAAGTTATGTGGGCTTTATTGAAGCTGCATCAGATAGAGGTCTGCTTGATGGGCCCAAATGTCCAAAGGCTCTGGGCGACTTGGTAGAGTCCTGTCTGGGTGCAATTCTTCTTGATACAGGGTTCAACTTGAATCGTGTTTGGGAGATAATGCTATCCTTTCTGAAACCAATTATGAGCTTTTCCAGCTTGCAACTTAGTCCTATTAGGGAACTACGTGAACTTTGCCAAGCTCATTCATGGGGTCTTGAGTTTGTACCGACAAAAATGGGTAAAAAGTATTCAGTTCGTGCAACAGTGAATGGGAATAATGTTTGTGTATCTGCTTCTACGACCAGCTTGAACAAAAAGGATGGAATAAGAATTTGTGCACAACTGATTTTCGAGAAGTTGAAGGCTCAAGGCAACATACCTATGATTAACTCATTGGAGGAAGTCTTAAAGTCAAGCAGCGAGATGGAAGCCAAACTTATCGGATATGATGAAACACCAATAGATGTTGTGCTTCTTGATGCAAGTGGATTTGATAACTTAAATGTACAAGAAGTTTCCCGCGGAAATTTTAACTCCCAAGGGCATCCTATCAATCGAGAGAGAAATGGTCACTCTTCCTGCAGAAAACCTGTACGACAATCCCCATCTTCATCTGGAGCAGTCAAAATGCGGCCCAATAAGACCAGGGAAAATCCTAAGAATGATGCTAACTCGCAGTCCGGAGGTACACACACGGCAACATCAAGATCTCGTTTGTACGAAGTTTGTGCCGCTAACTACTGGGAAACTCCCTTATTTGAGTGCTGCAATGAAGAAGGACCAAGCCACTTAAAATTGTTCACCTACAAGGCTGTAGTAAAGATAGAAGAAGATCCAGATACGCTGTTAGAGTGCTTCGGAGAGCCTAATATGAAAAAGAAAGCAGCAGCAGAGAGTGCGGCAGAAGGGGCACTTTGGTACTTGAAAAACAGAGGTTTTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006351 GO:0006353 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009798 GO:0009888 GO:0009892 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097659 GO:0098542 GO:0098586 GO:0098727 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1624

Amino Acids

181.64

Weight (kDa)

6.42

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 38 - 203 1.8e-15 Type III restriction enzyme, res subunit
DEAD PF00270 40 - 203 1.4e-19 DEAD/DEAH box helicase
Helicase_C PF00271 394 - 503 8.5e-18 Helicase conserved C-terminal domain
Dicer_dimer PF03368 570 - 655 5.8e-24 Dicer dimerisation domain
PAZ PF02170 858 - 978 4.1e-08 PAZ domain
Ribonucleas_3_3 PF14622 1022 - 1080 5.8e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 1023 - 1155 4.2e-18 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1211 - 1341 4.3e-14 Ribonuclease-III-like
Ribonuclease_3 PF00636 1231 - 1339 4.4e-21 Ribonuclease III domain
DND1_DSRM PF14709 1544 - 1618 1.3e-14 double strand RNA binding domain from DEAD END PROTEIN 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 767, 3615
Acc36I ACCTGC 1 cut(s) 2952
AccB1I GGYRCC 1 cut(s) 1931
AccBSI CCGCTC 1 cut(s) 3055
AccI GTMKAC 4 cut(s) 50, 673, 3231, 3510
AccII CGCG 1 cut(s) 4449
AccIII TCCGGA 1 cut(s) 4607
AclI AACGTT 1 cut(s) 2402
AclWI GGATC 7 cut(s) 229, 415, 2015, 2028, 2153, 3249, 4754
AcsI RAATTY 9 cut(s) 1775, 2120, 2305, 2348, 2711, 3294, 3601, 4257, 4453
AcuI CTGAAG 4 cut(s) 447, 1567, 2244, 2984
AcyI GRCGYC 1 cut(s) 111
AfiI CCNNNNNNNGG 7 cut(s) 106, 382, 614, 2996, 3069, 3751, 3886
AflII CTTAAG 1 cut(s) 3488
AjnI CCWGG 4 cut(s) 2506, 3048, 3772, 4571
AjuI GAANNNNNNNTTGG 8 cut(s) 306, 338, 1760, 1792, 2750, 2782, 3423, 3455
AleI CACNNNNGTG 1 cut(s) 3360
Alw21I GWGCWC 2 cut(s) 1510, 4267
Alw26I GTCTC 4 cut(s) 730, 1277, 1631, 3302
Alw44I GTGCAC 2 cut(s) 1506, 4263
AlwI GGATC 7 cut(s) 229, 415, 2015, 2028, 2153, 3249, 4754
AlwNI CAGNNNCTG 4 cut(s) 1475, 2749, 3008, 3803
Ama87I CYCGRG 2 cut(s) 100, 2674
Aor13HI TCCGGA 1 cut(s) 4607
AoxI GGCC 4 cut(s) 937, 3277, 3947, 4559
ApaI GGGCCC 1 cut(s) 3951
ApaLI GTGCAC 2 cut(s) 1506, 4263
ApoI RAATTY 9 cut(s) 1775, 2120, 2305, 2348, 2711, 3294, 3601, 4257, 4453
ArsI GACNNNNNNTTYG 2 cut(s) 433, 465
AspLEI GCGC 1 cut(s) 228
AsuC2I CCSGG 1 cut(s) 59
AsuHPI GGTGA 8 cut(s) 365, 1040, 1427, 3098, 3356, 3383, 3727, 4720
AsuII TTCGAA 2 cut(s) 83, 3516
AsuNHI GCTAGC 2 cut(s) 1481, 3205
AvaI CYCGRG 2 cut(s) 100, 2674
AvaII GGWCC 8 cut(s) 130, 758, 2089, 2254, 2695, 2813, 3843, 4706
AxyI CCTNAGG 1 cut(s) 2136
BaeGI GKGCMC 5 cut(s) 1510, 2681, 3951, 4267, 4840
BamHI GGATCC 1 cut(s) 2020
BanI GGYRCC 1 cut(s) 1931
BanII GRGCYC 2 cut(s) 2506, 3951
BarI GAAGNNNNNNTAC 2 cut(s) 420, 452
BauI CACGAG 1 cut(s) 1497
BbsI GAAGAC 2 cut(s) 857, 1983
Bbv12I GWGCWC 2 cut(s) 1510, 4267
BbvCI CCTCAGC 1 cut(s) 2102
BceAI ACGGC 5 cut(s) 188, 615, 3264, 3701, 4638
BcgI CGANNNNNNTGC 2 cut(s) 4637, 4671
BciT130I CCWGG 4 cut(s) 2508, 3050, 3774, 4573
BclI TGATCA 2 cut(s) 34, 3732
BcnI CCSGG 1 cut(s) 59
BcoDI GTCTC 4 cut(s) 730, 1277, 1631, 3302
BfmI CTRYAG 8 cut(s) 787, 1122, 2149, 2517, 2656, 3874, 4507, 4740
BfrI CTTAAG 1 cut(s) 3488
BfuAI ACCTGC 1 cut(s) 2952
BglII AGATCT 2 cut(s) 2234, 4634
BmcAI AGTACT 3 cut(s) 432, 1757, 3266
Bme1390I CCNGG 5 cut(s) 59, 2508, 3050, 3774, 4573
Bme18I GGWCC 8 cut(s) 130, 758, 2089, 2254, 2695, 2813, 3843, 4706
BmeT110I CYCGRG 2 cut(s) 100, 2674
BmiI GGNNCC 8 cut(s) 1140, 1933, 2022, 2178, 2256, 2505, 2814, 3949
BmrFI CCNGG 5 cut(s) 59, 2508, 3050, 3774, 4573
BmrI ACTGGG 4 cut(s) 391, 2180, 2486, 4678
BmtI GCTAGC 2 cut(s) 1485, 3209
BmuI ACTGGG 4 cut(s) 391, 2180, 2486, 4678
BpiI GAAGAC 2 cut(s) 857, 1983
BplI GAGNNNNNCTC 2 cut(s) 3621, 3653
BpmI CTGGAG 4 cut(s) 591, 1401, 2490, 4563
Bpu10I CCTNAGC 4 cut(s) 684, 2102, 2736, 2853
Bpu14I TTCGAA 2 cut(s) 83, 3516
BpuEI CTTGAG 8 cut(s) 495, 830, 1135, 1259, 2309, 3083, 4163, 4278
BpuMI CCSGG 1 cut(s) 59
BsaAI YACGTR 1 cut(s) 4112
BsaBI GATNNNNATC 5 cut(s) 1355, 1596, 2019, 2217, 3351
BsaHI GRCGYC 1 cut(s) 111
BsaJI CCNNGG 6 cut(s) 101, 322, 2072, 4447, 4465, 4572
BsaWI WCCGGW 2 cut(s) 68, 4607
BsaXI ACNNNNNCTCC 8 cut(s) 589, 619, 1973, 2003, 2988, 3018, 3435, 3465
Bsc4I CCNNNNNNNGG 7 cut(s) 106, 382, 614, 2996, 3069, 3751, 3886
Bse118I RCCGGY 1 cut(s) 2931
Bse21I CCTNAGG 1 cut(s) 2136
Bse3DI GCAATG 4 cut(s) 1101, 3414, 3585, 4702
Bse8I GATNNNNATC 5 cut(s) 1355, 1596, 2019, 2217, 3351
BseAI TCCGGA 1 cut(s) 4607
BseBI CCWGG 4 cut(s) 2508, 3050, 3774, 4573
BseDI CCNNGG 6 cut(s) 101, 322, 2072, 4447, 4465, 4572
BseJI GATNNNNATC 5 cut(s) 1355, 1596, 2019, 2217, 3351
BseLI CCNNNNNNNGG 7 cut(s) 106, 382, 614, 2996, 3069, 3751, 3886
BseMI GCAATG 4 cut(s) 1101, 3414, 3585, 4702
BseMII CTCAG 8 cut(s) 1073, 1532, 2116, 2127, 2727, 2844, 3030, 3275
BseRI GAGGAG 6 cut(s) 167, 1025, 2043, 2169, 2843, 3693
BseSI GKGCMC 5 cut(s) 1510, 2681, 3951, 4267, 4840
BsgI GTGCAG 2 cut(s) 2043, 3021
Bsh1236I CGCG 1 cut(s) 4449
BshFI GGCC 4 cut(s) 939, 3279, 3949, 4561
BshNI GGYRCC 1 cut(s) 1931
BsiHKAI GWGCWC 2 cut(s) 1510, 4267
BsiHKCI CYCGRG 2 cut(s) 100, 2674
BsiSI CCGG 5 cut(s) 58, 69, 89, 2932, 4608
BslFI GGGAC 6 cut(s) 190, 2240, 2479, 2585, 2826, 3168
BslI CCNNNNNNNGG 7 cut(s) 106, 382, 614, 2996, 3069, 3751, 3886
BsmAI GTCTC 4 cut(s) 730, 1277, 1631, 3302
BsmBI CGTCTC 1 cut(s) 1277
BsmFI GGGAC 6 cut(s) 190, 2240, 2479, 2585, 2826, 3168
BsmI GAATGC 2 cut(s) 906, 3446
BsnI GGCC 4 cut(s) 939, 3279, 3949, 4561
BsoBI CYCGRG 2 cut(s) 100, 2674
Bsp119I TTCGAA 2 cut(s) 83, 3516
Bsp120I GGGCCC 1 cut(s) 3947
Bsp1286I GDGCHC 6 cut(s) 1510, 2506, 2681, 3951, 4267, 4840
Bsp13I TCCGGA 1 cut(s) 4607
Bsp1407I TGTACA 2 cut(s) 3652, 4432
Bsp19I CCATGG 1 cut(s) 2072
BspANI GGCC 4 cut(s) 939, 3279, 3949, 4561
BspCNI CTCAG 8 cut(s) 1072, 1531, 2115, 2128, 2728, 2845, 3031, 3274
BspEI TCCGGA 1 cut(s) 4607
BspFNI CGCG 1 cut(s) 4449
BspHI TCATGA 1 cut(s) 3331
BspLI GGNNCC 8 cut(s) 1140, 1933, 2022, 2178, 2256, 2505, 2814, 3949
BspMAI CTGCAG 1 cut(s) 4511
BspMI ACCTGC 1 cut(s) 2952
BspOI GCTAGC 2 cut(s) 1485, 3209
BspPI GGATC 7 cut(s) 229, 415, 2015, 2028, 2153, 3249, 4754
BspT104I TTCGAA 2 cut(s) 83, 3516
BspT107I GGYRCC 1 cut(s) 1931
BspTI CTTAAG 1 cut(s) 3488
BsrBI CCGCTC 1 cut(s) 3055
BsrDI GCAATG 4 cut(s) 1101, 3414, 3585, 4702
BsrFI RCCGGY 1 cut(s) 2931
BsrGI TGTACA 2 cut(s) 3652, 4432
BssAI RCCGGY 1 cut(s) 2931
BssECI CCNNGG 6 cut(s) 101, 322, 2072, 4447, 4465, 4572
BssNAI GTATAC 2 cut(s) 674, 3232
BssNI GRCGYC 1 cut(s) 111
BssSI CACGAG 1 cut(s) 1497
BssT1I CCWWGG 3 cut(s) 322, 2072, 4465
Bst1107I GTATAC 2 cut(s) 674, 3232
Bst2BI CACGAG 1 cut(s) 1497
Bst2UI CCWGG 4 cut(s) 2508, 3050, 3774, 4573
Bst6I CTCTTC 1 cut(s) 4507
BstACI GRCGYC 1 cut(s) 111
BstAFI CTTAAG 1 cut(s) 3488
BstAPI GCANNNNNTGC 3 cut(s) 1264, 3008, 4823
BstAUI TGTACA 2 cut(s) 3652, 4432
BstBAI YACGTR 1 cut(s) 4112
BstBI TTCGAA 2 cut(s) 83, 3516
BstDSI CCRYGG 2 cut(s) 2072, 4447
BstEII GGTNACC 1 cut(s) 3389
BstENI CCTNNNNNAGG 1 cut(s) 3884
BstFNI CGCG 1 cut(s) 4449
BstHHI GCGC 1 cut(s) 228
BstMAI GTCTC 4 cut(s) 730, 1277, 1631, 3302
BstNI CCWGG 4 cut(s) 2508, 3050, 3774, 4573
BstNSI RCATGY 3 cut(s) 1443, 2972, 3543
BstPI GGTNACC 1 cut(s) 3389
BstSCI CCNGG 5 cut(s) 57, 2506, 3048, 3772, 4571
BstSFI CTRYAG 8 cut(s) 787, 1122, 2149, 2517, 2656, 3874, 4507, 4740
BstSLI GKGCMC 5 cut(s) 1510, 2681, 3951, 4267, 4840
BstUI CGCG 1 cut(s) 4449
BstV2I GAAGAC 2 cut(s) 857, 1983
BstX2I RGATCY 5 cut(s) 2020, 2145, 2234, 4634, 4759
BstYI RGATCY 5 cut(s) 2020, 2145, 2234, 4634, 4759
BstZ17I GTATAC 2 cut(s) 674, 3232
Bsu36I CCTNAGG 1 cut(s) 2136
BsuRI GGCC 4 cut(s) 939, 3279, 3949, 4561
BtgI CCRYGG 2 cut(s) 2072, 4447
BveI ACCTGC 1 cut(s) 2952
CaiI CAGNNNCTG 4 cut(s) 1475, 2749, 3008, 3803
CciI TCATGA 1 cut(s) 3331
CfoI GCGC 1 cut(s) 228
Cfr10I RCCGGY 1 cut(s) 2931
Cfr42I CCGCGG 1 cut(s) 4450
CseI GACGC 2 cut(s) 119, 782
CspCI CAANNNNNGTGG 2 cut(s) 1959, 1994
DraI TTTAAA 1 cut(s) 1134
Eam1104I CTCTTC 1 cut(s) 4507
EarI CTCTTC 1 cut(s) 4507
EciI GGCGGA 1 cut(s) 30
Eco130I CCWWGG 3 cut(s) 322, 2072, 4465
Eco24I GRGCYC 2 cut(s) 2506, 3951
Eco32I GATATC 1 cut(s) 1052
Eco47I GGWCC 8 cut(s) 130, 758, 2089, 2254, 2695, 2813, 3843, 4706
Eco57I CTGAAG 4 cut(s) 447, 1567, 2244, 2984
Eco81I CCTNAGG 1 cut(s) 2136
Eco88I CYCGRG 2 cut(s) 100, 2674
Eco91I GGTNACC 1 cut(s) 3389
EcoNI CCTNNNNNAGG 1 cut(s) 3884
EcoO109I RGGNCCY 1 cut(s) 2089
EcoO65I GGTNACC 1 cut(s) 3389
EcoRII CCWGG 4 cut(s) 2506, 3048, 3772, 4571
EcoRV GATATC 1 cut(s) 1052
EcoT14I CCWWGG 3 cut(s) 322, 2072, 4465
EcoT22I ATGCAT 5 cut(s) 656, 906, 1217, 1534, 3545
EcoT38I GRGCYC 2 cut(s) 2506, 3951
ErhI CCWWGG 3 cut(s) 322, 2072, 4465
Esp3I CGTCTC 1 cut(s) 1277
FalI AAGNNNNNCTT 6 cut(s) 306, 338, 4347, 4379, 4824, 4856
FaqI GGGAC 6 cut(s) 190, 2240, 2479, 2585, 2826, 3168
FauI CCCGC 2 cut(s) 203, 4454
FbaI TGATCA 2 cut(s) 34, 3732
FblI GTMKAC 4 cut(s) 50, 673, 3231, 3510
FriOI GRGCYC 2 cut(s) 2506, 3951
GlaI GCGC 1 cut(s) 227
GsuI CTGGAG 4 cut(s) 591, 1401, 2490, 4563
HaeIII GGCC 4 cut(s) 939, 3279, 3949, 4561
HapII CCGG 5 cut(s) 58, 69, 89, 2932, 4608
HgaI GACGC 2 cut(s) 119, 782
HhaI GCGC 1 cut(s) 228
Hin1I GRCGYC 1 cut(s) 111
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HincII GTYRAC 2 cut(s) 1318, 3457
HindII GTYRAC 2 cut(s) 1318, 3457
HindIII AAGCTT 3 cut(s) 144, 1739, 2155
HpaII CCGG 5 cut(s) 58, 69, 89, 2932, 4608
HphI GGTGA 8 cut(s) 365, 1040, 1427, 3098, 3356, 3383, 3727, 4720
Hpy99I CGWCG 1 cut(s) 776
HpyCH4IV ACGT 6 cut(s) 159, 1165, 1270, 2402, 3336, 4111
HpySE526I ACGT 6 cut(s) 159, 1165, 1270, 2402, 3336, 4111
Hsp92I GRCGYC 1 cut(s) 111
HspAI GCGC 1 cut(s) 226
Kpn2I TCCGGA 1 cut(s) 4607
Ksp22I TGATCA 2 cut(s) 34, 3732
KspI CCGCGG 1 cut(s) 4450
LmnI GCTCC 9 cut(s) 77, 947, 1138, 2182, 2509, 3052, 3317, 3443, 4544
MaeII ACGT 6 cut(s) 159, 1165, 1270, 2402, 3336, 4111
MbiI CCGCTC 1 cut(s) 3055
MfeI CAATTG 2 cut(s) 125, 362
MflI RGATCY 5 cut(s) 2020, 2145, 2234, 4634, 4759
MhlI GDGCHC 6 cut(s) 1510, 2506, 2681, 3951, 4267, 4840
MlyI GAGTC 5 cut(s) 479, 640, 1051, 1323, 3992
MmeI TCCRAC 2 cut(s) 1834, 3421
Mph1103I ATGCAT 5 cut(s) 656, 906, 1217, 1534, 3545
MroI TCCGGA 1 cut(s) 4607
MslI CAYNNNNRTG 7 cut(s) 464, 531, 743, 1607, 3330, 3360, 4307
MspA1I CMGCKG 1 cut(s) 4449
MspCI CTTAAG 1 cut(s) 3488
MspI CCGG 5 cut(s) 58, 69, 89, 2932, 4608
MspR9I CCNGG 5 cut(s) 59, 2508, 3050, 3774, 4573
MunI CAATTG 2 cut(s) 125, 362
Mva1269I GAATGC 2 cut(s) 906, 3446
MvaI CCWGG 4 cut(s) 2508, 3050, 3774, 4573
MvnI CGCG 1 cut(s) 4449
NciI CCSGG 1 cut(s) 59
NcoI CCATGG 1 cut(s) 2072
NheI GCTAGC 2 cut(s) 1481, 3205
NlaIV GGNNCC 8 cut(s) 1140, 1933, 2022, 2178, 2256, 2505, 2814, 3949
NmuCI GTSAC 3 cut(s) 449, 3389, 4496
NsiI ATGCAT 5 cut(s) 656, 906, 1217, 1534, 3545
NspI RCATGY 3 cut(s) 1443, 2972, 3543
NspV TTCGAA 2 cut(s) 83, 3516
OliI CACNNNNGTG 1 cut(s) 3360
PaeI GCATGC 1 cut(s) 3543
PagI TCATGA 1 cut(s) 3331
PctI GAATGC 2 cut(s) 906, 3446
PflFI GACNNNGTC 1 cut(s) 59
PfoI TCCNGGA 1 cut(s) 2506
PleI GAGTC 5 cut(s) 478, 639, 1051, 1322, 3991
PpsI GAGTC 5 cut(s) 478, 639, 1051, 1322, 3991
Ppu21I YACGTR 1 cut(s) 4112
PpuMI RGGWCCY 1 cut(s) 2089
PsiI TTATAA 2 cut(s) 767, 3615
Psp1406I AACGTT 1 cut(s) 2402
Psp5II RGGWCCY 1 cut(s) 2089
Psp6I CCWGG 4 cut(s) 2506, 3048, 3772, 4571
PspEI GGTNACC 1 cut(s) 3389
PspGI CCWGG 4 cut(s) 2506, 3048, 3772, 4571
PspN4I GGNNCC 8 cut(s) 1140, 1933, 2022, 2178, 2256, 2505, 2814, 3949
PspOMI GGGCCC 1 cut(s) 3947
PspPPI RGGWCCY 1 cut(s) 2089
PstI CTGCAG 1 cut(s) 4511
PstNI CAGNNNCTG 4 cut(s) 1475, 2749, 3008, 3803
PsuI RGATCY 5 cut(s) 2020, 2145, 2234, 4634, 4759
PsyI GACNNNGTC 1 cut(s) 59
RseI CAYNNNNRTG 7 cut(s) 464, 531, 743, 1607, 3330, 3360, 4307
SacII CCGCGG 1 cut(s) 4450
ScaI AGTACT 3 cut(s) 432, 1757, 3266
SchI GAGTC 5 cut(s) 479, 640, 1051, 1323, 3992
ScrFI CCNGG 5 cut(s) 59, 2508, 3050, 3774, 4573
SduI GDGCHC 6 cut(s) 1510, 2506, 2681, 3951, 4267, 4840
SfcI CTRYAG 8 cut(s) 787, 1122, 2149, 2517, 2656, 3874, 4507, 4740
Sfr303I CCGCGG 1 cut(s) 4450
SfuI TTCGAA 2 cut(s) 83, 3516
SgrBI CCGCGG 1 cut(s) 4450
SinI GGWCC 8 cut(s) 130, 758, 2089, 2254, 2695, 2813, 3843, 4706
SmiMI CAYNNNNRTG 7 cut(s) 464, 531, 743, 1607, 3330, 3360, 4307
SmlI CTYRAG 9 cut(s) 510, 809, 1150, 1274, 2324, 3098, 3488, 4142, 4293
SmoI CTYRAG 9 cut(s) 510, 809, 1150, 1274, 2324, 3098, 3488, 4142, 4293
SphI GCATGC 1 cut(s) 3543
StyD4I CCNGG 5 cut(s) 57, 2506, 3048, 3772, 4571
StyI CCWWGG 3 cut(s) 322, 2072, 4465
TaiI ACGT 6 cut(s) 162, 1168, 1273, 2405, 3339, 4114
TaqII GACCGA 3 cut(s) 2355, 2830, 3831
TatI WGTACW 6 cut(s) 430, 878, 1755, 3264, 3652, 4432
TauI GCSGC 4 cut(s) 3058, 4561, 4661, 4829
TseFI GTSAC 3 cut(s) 449, 3389, 4496
Tsp45I GTSAC 3 cut(s) 449, 3389, 4496
TspGWI ACGGA 2 cut(s) 1525, 3800
Tth111I GACNNNGTC 1 cut(s) 59
Vha464I CTTAAG 1 cut(s) 3488
VneI GTGCAC 2 cut(s) 1506, 4263
VpaK11BI GGWCC 8 cut(s) 130, 758, 2089, 2254, 2695, 2813, 3843, 4706
XagI CCTNNNNNAGG 1 cut(s) 3884
XapI RAATTY 9 cut(s) 1775, 2120, 2305, 2348, 2711, 3294, 3601, 4257, 4453
XbaI TCTAGA 3 cut(s) 1322, 3022, 3554
XceI RCATGY 3 cut(s) 1443, 2972, 3543
XcmI CCANNNNNNNNNTGG 2 cut(s) 283, 1850
XmiI GTMKAC 4 cut(s) 50, 673, 3231, 3510
ZrmI AGTACT 3 cut(s) 432, 1757, 3266
Zsp2I ATGCAT 5 cut(s) 656, 906, 1217, 1534, 3545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.