Rroxscaffold_2G00154590

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004795
Physical Location & Seq
Forward (+)
91159047 .. 91172323
13277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00154590.1

Sequence Viewer

Length: 4941 bp
ATGGACGGCGCGCAGCCCGATACGGGTCCTCCACCACCGCAGAAGGTGGGTCGGGCTGTGGAGTCGGGTGATGGAGCTCCGAGGCCGGAGAAGGATCCCAGGAAGGTTGCCCGGAGGTATCAATTGGAGCTGTGTCAGAAAGCTGTGCAGGAGAACATAATCGTCTATTTGGAAACTGGCTGTGGAAAGACCCACATAGCAGTGTTGCTTATCTATGAGCTGCGCGAGTTGATAATGAAACCTCATAAGAACATATGTGTGTTTCTTGCGCCTACGGTGGCGCTTGTTCAACAGCAAGCAAGAGTTATAGAAGATTCCCTTGATCTCAAGGTTGGGACCTATTGTGGAAGCTCTAAGAAATTGAAAACTCACCAAGACTGGGAAAAAGAGATGGAACAATATGAGGTTTTTGTCATGACACCTCAAATACTGTTACGTAACTTATACCACCGTATTATTAAGATGGAGTTGATTGCATTGCTGATTTTTGATGAGTGTCATCACGCTCAAGTGAAAAGCAACCATCCTTATGCAGAAATTATGAAATTTTACAAGAGTGATGCCACTAAACTTCCTCGTATATTTGGCATGACTGCATCTCCAGTTGTAGGTAAAGGCACTTCTAGTCAATCGAATTTATCAAAGAGTATCAATAGTCTTGAAAATTTACTTGATGCTAAGGTTTATACGGTTGAAGACAAGACAGAATTGCATTGTTCTGTTGTTTCTCCTGTTATCAATGTCCATCGTTATGCTCCTGTTATTAGTGGCACATCCAGCCCCTGCATGACTTTTTCTAGAAAACTTGACCAGCTGAAACGCCAGTGTATAGCGGAACTTAGTAAGAAGACGAGTGACTACCAAAGTCTAAGGAGTACAAAGAAAACGTTAAACAGAGTGCATGACAGTATAATATTTTGCTTGGAAAGTCTTGGCCTCTGGGGTGCGTTACAAGCTAGACATATTCTTTCGAGTGGTGATAACTTTGAACGAAATGAATTAATAGAGGAAGGCAATAATGATATAGAGGCAGAAGGAAATAATAGTGATGACTCTGTATGTGCGGAATATCTGGCTCAGGCAGCTGACATCATTCGTACTGACTGTGTGAAAGATGCTGTTGCATCTGGTCTATCTTGTGTAGAGATCTTAAGGGAGCCATTCTTCTCAAGTAAGGTCTTACGTCTTATTGGAATTCTTTCATCCTTCAGGTTGCAGCAGGATATGAAATGCATAATTTTTGTCAATAGGATTATTACTGCAAGATCCTTGACCTACATACTACAAAACCTTAAGAATCTAGCATCTTGGAAGTGCGATTTTCTTGTAGGAGTCCACTCGAAACTGAAGAGTATGTCAAGGAAGACGATGCAGATCATTCTTGATAAGTTTCGGTCTGGAGAGTTGAATCTATTGGTTGCAACTAAAGTTGGTGAAGAGGGACTAGATATTCAGACTTGCTGCCTCGTGATACGATTTGATCTTCCAGAAACTGTTGCTAGCTTTATACAGTCACGAGGTCGTGCACGGATGCCTCAGTCTGAATATATATTTTTGGTAAACAGTGGTAGTCAGAAAGAGTTGGATTTAATAGAAAACTTCAGGAAAGATGAAGAGCGAATGAACATGGAAGTTGCTTTCAGAACATCCAGTGAGACTTTTCTTAGTCCTGAGGAACGAACATATAAAGTTGCTTCTTCTGGAGCTTCCGTCACTTCTGGATATAGCATCTCATTGTTACACCTGTATTGTTCGAAGCTTCCACATGATGAGTATTATGTCCCCACTCCAGAATTCTATTTTCTTGGTGATTTGGAGGGAACTATTTGCCAGATAATTTTACCTTCCAATGCTCCAATGCATCAAATTGTCAGTACACCACAATCTTCGATGGAAGATGCCAAAAAAGATGCATGTCTAAAAGCCATCGAAGAATTGCATAAATTGGGTGCCTTAAGTGACTATCTCTTGCCACAGCAAGACAATGCAAATGTGGAGGAGTCCATGCTAGACTCTTCTGATTATGACAGCATGGAAGATGAAGACTTACGAGAGGAACTACATGAAATGCTGGTTCCTGCTGTCCTAAAGGAGTCATGGAATAAGTTGGAGGATCTCGTCACCCTCAGCGCTTACTATTTTCAATTTGATCCTGAACCTAATGATAGAATCTATAGAAGTTTTGGTCTATTTGTCAAAGCACCTCTTCCAGCAGAGGCCGAAACAATGGAACTTGATCTTCATCTCGCTCATGGTAGATCTGTGATGACAAAGTTGGTGCCATCAGGATTTGCAGAATTTGTCAAAGATGAGATCTTGCTGGCTCAAAACTTTCAAGAAATGTTCCTCAAGCTCATTCTGGACCGATCAGAATTTGTTTCTGAATTTGTTCCCCTGGGAAACTATGATTTTAGCAGATCGAGCACATCAACCTTCTACCTATTGCTTCCAGTAACTTTGGGTGAAAATGATAAGATAAGCATAGATTGGAGGATTATCAAAAAATGTTTATCATCTCCAATATTCAGGGGTCCAGGAGATGCTATGGACGGTAAAATTGTATCTTCAGGTATTCGACTTGCATCTGGTTATAGAAGCATCAGTGAAGTTGAGGATAGTTTAGTTTATGTCCCATATAAGAAGACATTTTACTTCATCACTAATGTTACTCGCGGAAGAAATGCCTACAGCCAATATAAAGAAAAGTCAGATGCTTTAACCTATGTGGATCATCTAAGTAAAAAGTTTGACATTTCTCTTAAATACCCTGAGCAACATCTTTTGTGTGCAAAACCAGTTTTCAATTTGCACAACTTGCTACACAATCGTAGGCAAGAAGATTCAGAAGCACAGCAACTAGATGAGTATTATATATATGTGCCTCCTGAGCTTTGCGAGCTGAAGGTAATTGGCTTTTCAAAAGATATTGGGAGTTCGGTATCTCTGTTACCATCAATCATGCACCGTCTGGAAAACTTGCTTGTCGCCATCGAACTGAAGCATGTATTATGCACTTCATTCCCTGAAGGAGCTGAGGTTACTTCGCAGAGAGTTCTAGAAGCCCTCACAACTGAGAAGTGCCAGGAGCGGATTTCCCTTGAAAGGCTTGAAATACTAGGAGACGCTTTCCTCAAATTTGCAGTTGGAAGGCACTTTTTCCTTTTGCATGCTTCACTTGACGAAGGTGAACTAACAAGGAAACGTTCAAATGTGGTAAACAATTCCAATTTATTGAAGCTAGCTACTAGAAGCAACTTACAGGTATACATACGGGATCAGCCATTTGAACCCTCTCAGTTCTTCGCTTTAGGCCGCCCTTGCAAAAATATTTGTGACCAGGAGACAATAGGATCTCTTGATTCTCAAGATTTAAGCAGTGCAGTAAAACAGTCACATGCTCGTCATGTCAGATGTAGTAAGGGTCACCATTGGTTACATAAGAAAACAATTGCTGATGTTGTTGAAGCCCTTGTTGGAGCATTCATAGTTGACAGCGGTTTTAAAGCAGCAACTGCATTTCTCAGATGGATTGGCATAAAAGTAAACTTTGAAGCATCAGAAGTTACTAAAGTATGCATAGCAAGCAGCAGATACGTTTCACTTGCTGCTTGCATAGACATTGCGGCCCTTGAAAATTCATTAGGGTATCAGTTTCTCCACAGAGGTTTGCTGTTACAGGCATTTGTACATCCTTCTTACAACAAACATGGAGGAGGTTGCTATCAGAGATTGGAGTTTCTTGGAGATGCTGTCTTGGATTACTTGATCACCTCATACCTGTATTCAGTGTATCCAAAGTTAAAGCCTGGCCATTTGACAGATTTGAGATCAATTGCTGTGAATAATAAAGCCTTTGCTACTGTTGCAGTAGCTCGATCCTTCCACAAGTTTCTACTCTCTGACACCAGTACCCTTTCTGAGGCTATAAAGACTTATGTGAACTTCATTGAGACATCTGCATCAGATAGCAGTCTGGTTGATGGACCAAAATGCCCCAAGGCTCTGGGTGACTTGGTAGAGTCTTGTCTTGGTGCAATTCTTCTTGATACAGGGTTCGACTTAAACCTTGTTTGGAAGATAATGCTATCCTTTCTGGAGCCTGTCATGAGCTTTTCCAACATTCAGCTTAGTCCTGTTAGGGAACTACGGGAACTTTGCCAAGCTCATGCATGGGTTCTTAAGTTTTTACCATCAAAGAAGGGTAAAACATTTTCAGTTGAGGCAACAGTGAATGGGAAAAATGTTTCTGCAACTGCTTCCTCAACTGCCCTGAACAAAAAGGAGGCTATTAGAATTTCTGCTCAGTTAATTTTCGAAACGTTGAAGGCTCAAGGCAATATACCAAAGTCTAAGCTGTCTTTGGAGGAAGTCTTGAAGTCAAGCTGCAAGTTGGAACCCAAACTTATCGGATATGATGAAACACCAATAGATGTTACAGCTCTTGATGTTATTGGATTTGATAAGTTAAACGTACAAGAGGCTTCTAGCAGCAGTTCCAACTCAGATGTGCATTCTATCGGTGAAGTTAGCAGTGGTAACTCCTCCTGCATAAAACGTTTACGACAACCCCCAGCTTCACTTGGAGAAGTCAAAATGGACAGTACTAATGCCTGTAACAGTCGTAGCTCTGATGCTATCTCACAATCCAGAGGTGTGCCACACAAATTAACAGCAAAATCTCGTTTGTATGAAACCTGTGTTGCTAACTACTGGGAACCCCCTGAATTTGAATGTTGCAAAGAGGAAGGACCAGGCCACATAAAATCGTTCACTTTCAAGGTTACAGTGAAGATAGATGATGTTTCGGATACACTTTTGGAGGCCTTTGGAGAACCTCGTACGAGCAAGAAAGCAGCAGCAGAGCATGCAGCGGAAGGGGCACTTTGGTACTTGGAAAAGAAAGGTTACATAGAGGAGAGTGATCAATCAGAGGAACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006351 GO:0006353 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009798 GO:0009888 GO:0009892 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097659 GO:0098542 GO:0098586 GO:0098727 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1646

Amino Acids

184.31

Weight (kDa)

6.02

Isoelectric Point (pI)

49.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 38 - 201 3.5e-17 Type III restriction enzyme, res subunit
DEAD PF00270 39 - 201 4.5e-19 DEAD/DEAH box helicase
Helicase_C PF00271 396 - 509 9.2e-18 Helicase conserved C-terminal domain
Dicer_dimer PF03368 576 - 661 3e-23 Dicer dimerisation domain
PAZ PF02170 869 - 989 1.2e-08 PAZ domain
Ribonucleas_3_3 PF14622 1033 - 1091 5.5e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 1034 - 1166 4.1e-18 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1222 - 1353 7.9e-14 Ribonuclease-III-like
Ribonuclease_3 PF00636 1242 - 1351 3.6e-21 Ribonuclease III domain
DND1_DSRM PF14709 1557 - 1632 1.6e-13 double strand RNA binding domain from DEAD END PROTEIN 1
dsrm PF00035 1558 - 1628 7.1e-07 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1949, 2278
AccBSI CCGCTC 1 cut(s) 3088
AccI GTMKAC 1 cut(s) 3264
AccII CGCG 3 cut(s) 11, 225, 2673
AciI CCGC 9 cut(s) 38, 833, 1064, 2673, 3088, 3313, 3495, 3623, 4871
AclI AACGTT 4 cut(s) 887, 3202, 4319, 4555
AclWI GGATC 9 cut(s) 89, 102, 1260, 2121, 2144, 2736, 3282, 3358, 3870
AcoI YGGCCR 1 cut(s) 3808
AcuI CTGAAG 7 cut(s) 1192, 1367, 1585, 2550, 2921, 3017, 3045
AfaI GTAC 9 cut(s) 877, 1099, 1876, 3687, 3910, 4473, 4603, 4840, 4889
AfeI AGCGCT 1 cut(s) 2131
AfiI CCNNNNNNNGG 5 cut(s) 23, 379, 608, 3102, 3919
AflII CTTAAG 4 cut(s) 1150, 1292, 1954, 4178
AjnI CCWGG 7 cut(s) 98, 2394, 2533, 3081, 3336, 3805, 4750
AjuI GAANNNNNNNTTGG 2 cut(s) 3456, 3488
Alw21I GWGCWC 3 cut(s) 79, 1528, 2426
Alw26I GTCTC 4 cut(s) 1649, 3114, 3335, 3944
Alw44I GTGCAC 1 cut(s) 1524
AlwI GGATC 9 cut(s) 89, 102, 1260, 2121, 2144, 2736, 3282, 3358, 3870
AlwNI CAGNNNCTG 3 cut(s) 783, 1493, 3512
Aor51HI AGCGCT 1 cut(s) 2131
AoxI GGCC 8 cut(s) 83, 934, 2217, 3310, 3624, 3808, 4753, 4821
ApaLI GTGCAC 1 cut(s) 1524
AseI ATTAAT 1 cut(s) 1001
Asp700I GAANNNNTTC 2 cut(s) 1198, 2388
AspLEI GCGC 6 cut(s) 11, 13, 225, 271, 283, 2132
AspS9I GGNCC 7 cut(s) 26, 336, 2362, 2531, 3625, 3983, 4748
AsuC2I CCSGG 1 cut(s) 112
AsuII TTCGAA 2 cut(s) 1754, 4314
AsuNHI GCTAGC 2 cut(s) 1499, 3238
AvaII GGWCC 6 cut(s) 26, 336, 2362, 2531, 3983, 4748
AxyI CCTNAGG 1 cut(s) 1671
BaeGI GKGCMC 2 cut(s) 1528, 4882
BaeI ACNNNNGTAYC 2 cut(s) 3892, 3925
BalI TGGCCA 1 cut(s) 3810
BamHI GGATCC 1 cut(s) 94
BanI GGYRCC 2 cut(s) 1949, 2278
BanII GRGCYC 1 cut(s) 79
BarI GAAGNNNNNNTAC 4 cut(s) 604, 636, 2633, 2665
BauI CACGAG 2 cut(s) 1466, 1515
BbsI GAAGAC 5 cut(s) 702, 854, 1370, 2049, 2648
Bbv12I GWGCWC 3 cut(s) 79, 1528, 2426
BbvCI CCTCAGC 2 cut(s) 2126, 3033
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 7 cut(s) 100, 2396, 2535, 3083, 3338, 3807, 4752
BciVI GTATCC 2 cut(s) 3801, 4801
BclI TGATCA 2 cut(s) 3765, 4921
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 4 cut(s) 1649, 3114, 3335, 3944
BfmI CTRYAG 2 cut(s) 2173, 2686
BfoI RGCGCY 2 cut(s) 284, 2133
BfrI CTTAAG 4 cut(s) 1150, 1292, 1954, 4178
BfuI GTATCC 2 cut(s) 3801, 4801
BglII AGATCT 3 cut(s) 1146, 2258, 2313
BmcAI AGTACT 1 cut(s) 4603
Bme1390I CCNGG 8 cut(s) 100, 112, 2396, 2535, 3083, 3338, 3807, 4752
Bme18I GGWCC 6 cut(s) 26, 336, 2362, 2531, 3983, 4748
BmgT120I GGNCC 7 cut(s) 26, 336, 2362, 2531, 3625, 3983, 4748
BmrFI CCNGG 8 cut(s) 100, 112, 2396, 2535, 3083, 3338, 3807, 4752
BmrI ACTGGG 2 cut(s) 388, 4720
BmtI GCTAGC 2 cut(s) 1503, 3242
BmuI ACTGGG 2 cut(s) 388, 4720
BpiI GAAGAC 5 cut(s) 702, 854, 1370, 2049, 2648
BpmI CTGGAG 5 cut(s) 585, 1419, 1722, 1773, 4115
Bpu10I CCTNAGC 6 cut(s) 678, 1077, 2126, 2769, 2886, 3033
Bpu14I TTCGAA 2 cut(s) 1754, 4314
BpuEI CTTGAG 6 cut(s) 311, 492, 1153, 2333, 3348, 4314
BpuMI CCSGG 1 cut(s) 112
BsaAI YACGTR 1 cut(s) 437
BsaBI GATNNNNATC 2 cut(s) 1373, 2241
BsaJI CCNNGG 5 cut(s) 80, 98, 2394, 2395, 3996
Bsc4I CCNNNNNNNGG 5 cut(s) 23, 379, 608, 3102, 3919
Bse1I ACTGG 9 cut(s) 181, 383, 602, 823, 1650, 2450, 2795, 3906, 4715
Bse21I CCTNAGG 1 cut(s) 1671
Bse3DI GCAATG 2 cut(s) 476, 3618
Bse8I GATNNNNATC 2 cut(s) 1373, 2241
BseBI CCWGG 7 cut(s) 100, 2396, 2535, 3083, 3338, 3807, 4752
BseDI CCNNGG 5 cut(s) 80, 98, 2394, 2395, 3996
BseGI GGATG 6 cut(s) 523, 773, 1202, 1536, 1646, 3688
BseJI GATNNNNATC 2 cut(s) 1373, 2241
BseLI CCNNNNNNNGG 5 cut(s) 23, 379, 608, 3102, 3919
BseMI GCAATG 2 cut(s) 476, 3618
BseNI ACTGG 9 cut(s) 181, 383, 602, 823, 1650, 2450, 2795, 3906, 4715
BsePI GCGCGC 1 cut(s) 9
BseRI GAGGAG 4 cut(s) 2012, 3726, 4531, 4928
BseSI GKGCMC 2 cut(s) 1528, 4882
BseYI CCCAGC 1 cut(s) 4570
BsgI GTGCAG 2 cut(s) 167, 3399
Bsh1236I CGCG 3 cut(s) 11, 225, 2673
BshFI GGCC 8 cut(s) 85, 936, 2219, 3312, 3626, 3810, 4755, 4823
BshNI GGYRCC 2 cut(s) 1949, 2278
BsiHKAI GWGCWC 3 cut(s) 79, 1528, 2426
BsiSI CCGG 2 cut(s) 86, 112
BsiWI CGTACG 1 cut(s) 4838
BslFI GGGAC 4 cut(s) 349, 1455, 1766, 2615
BslI CCNNNNNNNGG 5 cut(s) 23, 379, 608, 3102, 3919
BsmAI GTCTC 4 cut(s) 1649, 3114, 3335, 3944
BsmBI CGTCTC 1 cut(s) 3114
BsmFI GGGAC 4 cut(s) 349, 1455, 1766, 2615
BsmI GAATGC 2 cut(s) 3479, 4510
BsnI GGCC 8 cut(s) 85, 936, 2219, 3312, 3626, 3810, 4755, 4823
Bsp119I TTCGAA 2 cut(s) 1754, 4314
Bsp1286I GDGCHC 4 cut(s) 79, 1528, 2426, 4882
Bsp1407I TGTACA 1 cut(s) 3685
BspACI CCGC 9 cut(s) 38, 833, 1064, 2673, 3088, 3313, 3495, 3623, 4871
BspANI GGCC 8 cut(s) 85, 936, 2219, 3312, 3626, 3810, 4755, 4823
BspFNI CGCG 3 cut(s) 11, 225, 2673
BspHI TCATGA 2 cut(s) 414, 4104
BspOI GCTAGC 2 cut(s) 1503, 3242
BspPI GGATC 9 cut(s) 89, 102, 1260, 2121, 2144, 2736, 3282, 3358, 3870
BspQI GCTCTTC 1 cut(s) 1608
BspT104I TTCGAA 2 cut(s) 1754, 4314
BspT107I GGYRCC 2 cut(s) 1949, 2278
BspTI CTTAAG 4 cut(s) 1150, 1292, 1954, 4178
BsrBI CCGCTC 1 cut(s) 3088
BsrDI GCAATG 2 cut(s) 476, 3618
BsrGI TGTACA 1 cut(s) 3685
BsrI ACTGG 9 cut(s) 181, 383, 602, 823, 1650, 2450, 2795, 3906, 4715
BssECI CCNNGG 5 cut(s) 80, 98, 2394, 2395, 3996
BssHII GCGCGC 1 cut(s) 9
BssNAI GTATAC 1 cut(s) 3265
BssSI CACGAG 2 cut(s) 1466, 1515
BssT1I CCWWGG 1 cut(s) 3996
Bst1107I GTATAC 1 cut(s) 3265
Bst2BI CACGAG 2 cut(s) 1466, 1515
Bst2UI CCWGG 7 cut(s) 100, 2396, 2535, 3083, 3338, 3807, 4752
Bst6I CTCTTC 5 cut(s) 1343, 1431, 1608, 2020, 2211
BstAFI CTTAAG 4 cut(s) 1150, 1292, 1954, 4178
BstAPI GCANNNNNTGC 3 cut(s) 2815, 3512, 4865
BstAUI TGTACA 1 cut(s) 3685
BstBAI YACGTR 1 cut(s) 437
BstBI TTCGAA 2 cut(s) 1754, 4314
BstEII GGTNACC 1 cut(s) 3422
BstENI CCTNNNNNAGG 1 cut(s) 3917
BstF5I GGATG 6 cut(s) 523, 773, 1202, 1536, 1646, 3688
BstFNI CGCG 3 cut(s) 11, 225, 2673
BstH2I RGCGCY 2 cut(s) 284, 2133
BstHHI GCGC 6 cut(s) 11, 13, 225, 271, 283, 2132
BstMAI GTCTC 4 cut(s) 1649, 3114, 3335, 3944
BstNI CCWGG 7 cut(s) 100, 2396, 2535, 3083, 3338, 3807, 4752
BstNSI RCATGY 5 cut(s) 1917, 3005, 3170, 3398, 4868
BstPI GGTNACC 1 cut(s) 3422
BstSCI CCNGG 8 cut(s) 98, 110, 2394, 2533, 3081, 3336, 3805, 4750
BstSFI CTRYAG 2 cut(s) 2173, 2686
BstSLI GKGCMC 2 cut(s) 1528, 4882
BstSNI TACGTA 1 cut(s) 437
BstUI CGCG 3 cut(s) 11, 225, 2673
BstV2I GAAGAC 5 cut(s) 702, 854, 1370, 2049, 2648
BstX2I RGATCY 7 cut(s) 94, 1146, 1265, 2113, 2258, 2313, 3350
BstXI CCANNNNNNTGG 1 cut(s) 4003
BstYI RGATCY 7 cut(s) 94, 1146, 1265, 2113, 2258, 2313, 3350
BstZ17I GTATAC 1 cut(s) 3265
Bsu36I CCTNAGG 1 cut(s) 1671
BsuI GTATCC 2 cut(s) 3801, 4801
BsuRI GGCC 8 cut(s) 85, 936, 2219, 3312, 3626, 3810, 4755, 4823
BtsCI GGATG 6 cut(s) 523, 773, 1202, 1536, 1646, 3688
BtsI GCAGTG 3 cut(s) 207, 3382, 4537
CaiI CAGNNNCTG 3 cut(s) 783, 1493, 3512
CciI TCATGA 2 cut(s) 414, 4104
CfoI GCGC 6 cut(s) 11, 13, 225, 271, 283, 2132
Cfr13I GGNCC 7 cut(s) 26, 336, 2362, 2531, 3625, 3983, 4748
CseI GACGC 1 cut(s) 3131
Csp6I GTAC 9 cut(s) 876, 1098, 1875, 3686, 3909, 4472, 4602, 4839, 4888
CviQI GTAC 9 cut(s) 876, 1098, 1875, 3686, 3909, 4472, 4602, 4839, 4888
DraI TTTAAA 1 cut(s) 3502
EaeI YGGCCR 1 cut(s) 3808
Eam1104I CTCTTC 5 cut(s) 1343, 1431, 1608, 2020, 2211
EarI CTCTTC 5 cut(s) 1343, 1431, 1608, 2020, 2211
Ecl136II GAGCTC 1 cut(s) 77
Eco105I TACGTA 1 cut(s) 437
Eco130I CCWWGG 1 cut(s) 3996
Eco147I AGGCCT 1 cut(s) 4823
Eco24I GRGCYC 1 cut(s) 79
Eco47I GGWCC 6 cut(s) 26, 336, 2362, 2531, 3983, 4748
Eco47III AGCGCT 1 cut(s) 2131
Eco53kI GAGCTC 1 cut(s) 77
Eco57I CTGAAG 7 cut(s) 1192, 1367, 1585, 2550, 2921, 3017, 3045
Eco81I CCTNAGG 1 cut(s) 1671
Eco91I GGTNACC 1 cut(s) 3422
EcoICRI GAGCTC 1 cut(s) 77
EcoNI CCTNNNNNAGG 1 cut(s) 3917
EcoO109I RGGNCCY 2 cut(s) 26, 336
EcoO65I GGTNACC 1 cut(s) 3422
EcoRI GAATTC 2 cut(s) 1194, 1793
EcoRII CCWGG 7 cut(s) 98, 2394, 2533, 3081, 3336, 3805, 4750
EcoT14I CCWWGG 1 cut(s) 3996
EcoT22I ATGCAT 5 cut(s) 1235, 1863, 1915, 3578, 4171
EcoT38I GRGCYC 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 3996
Esp3I CGTCTC 1 cut(s) 3114
FalI AAGNNNNNCTT 6 cut(s) 303, 335, 2190, 2222, 4866, 4898
FaqI GGGAC 4 cut(s) 349, 1455, 1766, 2615
FauNDI CATATG 1 cut(s) 254
FbaI TGATCA 2 cut(s) 3765, 4921
FblI GTMKAC 1 cut(s) 3264
FokI GGATG 6 cut(s) 510, 760, 1189, 1543, 1633, 3675
FriOI GRGCYC 1 cut(s) 79
GlaI GCGC 6 cut(s) 10, 12, 224, 270, 282, 2131
GsaI CCCAGC 1 cut(s) 4574
GsuI CTGGAG 5 cut(s) 585, 1419, 1722, 1773, 4115
HaeII RGCGCY 2 cut(s) 284, 2133
HaeIII GGCC 8 cut(s) 85, 936, 2219, 3312, 3626, 3810, 4755, 4823
HapII CCGG 2 cut(s) 86, 112
HgaI GACGC 1 cut(s) 3131
HhaI GCGC 6 cut(s) 11, 13, 225, 271, 283, 2132
Hin6I GCGC 6 cut(s) 9, 11, 223, 269, 281, 2130
HinP1I GCGC 6 cut(s) 9, 11, 223, 269, 281, 2130
HincII GTYRAC 1 cut(s) 3490
HindII GTYRAC 1 cut(s) 3490
HindIII AAGCTT 1 cut(s) 1757
HpaII CCGG 2 cut(s) 86, 112
HpyCH4IV ACGT 8 cut(s) 436, 887, 1183, 3202, 3594, 4319, 4470, 4555
HpySE526I ACGT 8 cut(s) 436, 887, 1183, 3202, 3594, 4319, 4470, 4555
HspAI GCGC 6 cut(s) 9, 11, 223, 269, 281, 2130
Ksp22I TGATCA 2 cut(s) 3765, 4921
LguI GCTCTTC 1 cut(s) 1608
MaeII ACGT 8 cut(s) 436, 887, 1183, 3202, 3594, 4319, 4470, 4555
MbiI CCGCTC 1 cut(s) 3088
MfeI CAATTG 3 cut(s) 122, 3447, 3831
MflI RGATCY 7 cut(s) 94, 1146, 1265, 2113, 2258, 2313, 3350
MhlI GDGCHC 4 cut(s) 79, 1528, 2426, 4882
MlsI TGGCCA 1 cut(s) 3810
MluNI TGGCCA 1 cut(s) 3810
MlyI GAGTC 7 cut(s) 71, 1046, 1341, 2006, 2009, 2102, 4028
MmeI TCCRAC 7 cut(s) 1563, 2088, 3124, 3454, 4140, 4371, 4521
Mox20I TGGCCA 1 cut(s) 3810
Mph1103I ATGCAT 5 cut(s) 1235, 1863, 1915, 3578, 4171
MroXI GAANNNNTTC 2 cut(s) 1198, 2388
MscI TGGCCA 1 cut(s) 3810
MslI CAYNNNNRTG 4 cut(s) 200, 257, 528, 750
Msp20I TGGCCA 1 cut(s) 3810
MspA1I CMGCKG 4 cut(s) 814, 1085, 3495, 4871
MspCI CTTAAG 4 cut(s) 1150, 1292, 1954, 4178
MspI CCGG 2 cut(s) 86, 112
MspR9I CCNGG 8 cut(s) 100, 112, 2396, 2535, 3083, 3338, 3807, 4752
MunI CAATTG 3 cut(s) 122, 3447, 3831
Mva1269I GAATGC 2 cut(s) 3479, 4510
MvaI CCWGG 7 cut(s) 100, 2396, 2535, 3083, 3338, 3807, 4752
MvnI CGCG 3 cut(s) 11, 225, 2673
NciI CCSGG 1 cut(s) 112
NdeI CATATG 1 cut(s) 254
NheI GCTAGC 2 cut(s) 1499, 3238
NmuCI GTSAC 9 cut(s) 854, 1512, 1711, 1958, 2119, 3332, 3390, 3422, 4007
NsiI ATGCAT 5 cut(s) 1235, 1863, 1915, 3578, 4171
NspI RCATGY 5 cut(s) 1917, 3005, 3170, 3398, 4868
NspV TTCGAA 2 cut(s) 1754, 4314
PaeI GCATGC 2 cut(s) 3170, 4868
PagI TCATGA 2 cut(s) 414, 4104
PasI CCCWGGG 1 cut(s) 2395
PauI GCGCGC 1 cut(s) 9
PceI AGGCCT 1 cut(s) 4823
PciSI GCTCTTC 1 cut(s) 1608
PctI GAATGC 2 cut(s) 3479, 4510
PdmI GAANNNNTTC 2 cut(s) 1198, 2388
PfeI GAWTC 6 cut(s) 314, 1297, 1408, 2169, 2840, 3359
Pfl23II CGTACG 1 cut(s) 4838
PfoI TCCNGGA 1 cut(s) 2533
PleI GAGTC 7 cut(s) 70, 1046, 1340, 2006, 2008, 2101, 4027
PpsI GAGTC 7 cut(s) 70, 1046, 1340, 2006, 2008, 2101, 4027
Ppu21I YACGTR 1 cut(s) 437
PpuMI RGGWCCY 2 cut(s) 26, 336
PshBI ATTAAT 1 cut(s) 1001
Psp124BI GAGCTC 1 cut(s) 79
Psp1406I AACGTT 4 cut(s) 887, 3202, 4319, 4555
Psp5II RGGWCCY 2 cut(s) 26, 336
Psp6I CCWGG 7 cut(s) 98, 2394, 2533, 3081, 3336, 3805, 4750
PspEI GGTNACC 1 cut(s) 3422
PspFI CCCAGC 1 cut(s) 4570
PspGI CCWGG 7 cut(s) 98, 2394, 2533, 3081, 3336, 3805, 4750
PspLI CGTACG 1 cut(s) 4838
PspPI GGNCC 7 cut(s) 26, 336, 2362, 2531, 3625, 3983, 4748
PspPPI RGGWCCY 2 cut(s) 26, 336
PstNI CAGNNNCTG 3 cut(s) 783, 1493, 3512
PsuI RGATCY 7 cut(s) 94, 1146, 1265, 2113, 2258, 2313, 3350
PteI GCGCGC 1 cut(s) 9
PvuII CAGCTG 2 cut(s) 814, 1085
RsaI GTAC 9 cut(s) 877, 1099, 1876, 3687, 3910, 4473, 4603, 4840, 4889
RsaNI GTAC 9 cut(s) 876, 1098, 1875, 3686, 3909, 4472, 4602, 4839, 4888
RseI CAYNNNNRTG 4 cut(s) 200, 257, 528, 750
SacI GAGCTC 1 cut(s) 79
SapI GCTCTTC 1 cut(s) 1608
Sau96I GGNCC 7 cut(s) 26, 336, 2362, 2531, 3625, 3983, 4748
ScaI AGTACT 1 cut(s) 4603
SchI GAGTC 7 cut(s) 71, 1046, 1341, 2006, 2009, 2102, 4028
ScrFI CCNGG 8 cut(s) 100, 112, 2396, 2535, 3083, 3338, 3807, 4752
SduI GDGCHC 4 cut(s) 79, 1528, 2426, 4882
SfcI CTRYAG 2 cut(s) 2173, 2686
SfuI TTCGAA 2 cut(s) 1754, 4314
SinI GGWCC 6 cut(s) 26, 336, 2362, 2531, 3983, 4748
SmiMI CAYNNNNRTG 4 cut(s) 200, 257, 528, 750
SnaBI TACGTA 1 cut(s) 437
SphI GCATGC 2 cut(s) 3170, 4868
SseBI AGGCCT 1 cut(s) 4823
SsiI CCGC 9 cut(s) 38, 833, 1064, 2673, 3088, 3313, 3495, 3623, 4871
SspI AATATT 3 cut(s) 915, 2523, 3328
SstI GAGCTC 1 cut(s) 79
StuI AGGCCT 1 cut(s) 4823
StyD4I CCNGG 8 cut(s) 98, 110, 2394, 2533, 3081, 3336, 3805, 4750
StyI CCWWGG 1 cut(s) 3996
TaiI ACGT 8 cut(s) 439, 890, 1186, 3205, 3597, 4322, 4473, 4558
TaqII GACCGA 2 cut(s) 1383, 2379
TatI WGTACW 4 cut(s) 875, 1874, 3685, 4601
TauI GCSGC 2 cut(s) 3315, 3626
TfiI GAWTC 6 cut(s) 314, 1297, 1408, 2169, 2840, 3359
TseFI GTSAC 9 cut(s) 854, 1512, 1711, 1958, 2119, 3332, 3390, 3422, 4007
Tsp45I GTSAC 9 cut(s) 854, 1512, 1711, 1958, 2119, 3332, 3390, 3422, 4007
TspGWI ACGGA 2 cut(s) 1543, 1699
Vha464I CTTAAG 4 cut(s) 1150, 1292, 1954, 4178
VneI GTGCAC 1 cut(s) 1524
VpaK11BI GGWCC 6 cut(s) 26, 336, 2362, 2531, 3983, 4748
VspI ATTAAT 1 cut(s) 1001
XagI CCTNNNNNAGG 1 cut(s) 3917
XbaI TCTAGA 2 cut(s) 797, 3055
XceI RCATGY 5 cut(s) 1917, 3005, 3170, 3398, 4868
XmiI GTMKAC 1 cut(s) 3264
XmnI GAANNNNTTC 2 cut(s) 1198, 2388
ZrmI AGTACT 1 cut(s) 4603
Zsp2I ATGCAT 5 cut(s) 1235, 1863, 1915, 3578, 4171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.