RLG00000015757

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004795
Physical Location & Seq
Reverse (-)
1216302 .. 1228899
12598 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015757

Sequence Viewer

Length: 4950 bp
ATGAACGACGCGCCGCCCGATACGGGTCCTCCACCGCTGCAGAACGGTGGGGAAGAGGAGGGTCGGGCTGTGGAGTCGGGTGATGGAGCTCCGAGGCCGGAGAAGGATCCCAGGAGGTTTGCCCGGAGGTATCAATTGGAGCTGTGTCAGAAAGCTGTGGAAGAGAACATAATCGTGTATTTGGAAACTGGTTGTGGAAAGACCCACATAGCAGTGTTGCTCATGTATGAGCTGCGCGAGTTGATAATGAAACCTCGTAAGAGCATATGTGTGTTTCTTGCGCCTACGGTGGCGCTTGTTCAACAGCAAGCAAGAGTTATAGAAGATTCCCTTGATCTCAAGGTTGGGACCTATTGTGGAAGCTCTAAGAAATTGAAAACTCACCAAGACTGGGAAAAAGAGATGGAACAATATGAGGTTTTTGTCATGACGCCTCAAATACTGTTACGTAACTTATACCACCGTATTATTAAGATGGAGTTGATTGCATTGCTGATTTTTGATGAGTGTCATCACGCTCAAATGAAAAGCAACCATCCTTATGCAGAAATTATGAAATTTTACAAGAGTGATGCCACTAAACTTCCTCGTATATTTGGCATGACTGCATCTCCAGTTGTAGGTAAAGGCGCTTCTAGTCAATCGAATTTATCAAAGAGTATCAATAGTCTTGAAAATTTACTTGATGCTAAGGTTTATACGGTTGAAGACAAGACAGAATTGCATTGTTCTGTTGTTTCTCCTGTTATCAATGTCCATCGTTATGGTCCTGTTATTAGTGGCACATCCAGCCTCTGCATGACTTTTTCTAGAAAACTTGACCAGCTGAAACGCCAGTGTATAGCGGAACTTAGTAAGAAGACGAGTGACTACCAAAGTCTAAGGAGTACAAAGAAAACACTAAACAGAGTGCATGACAGTATAATATTTTGCTTGGAAAGTCTTGGCCTCTGGGGTGCGTTACAAGCTAGACATATTCTTTCGAGTGGCGATAACTTTGAACGAAATGAATTAATAGAGGAAGGCAATAATGATATAGAGGTAGAAGGAAATAATAGTGATGATTCTGTATGTGCGGAATATCTGGCTAAGGCAGCCGACATCATTCGTACTGACTGTGAGAAAGATGCTGTTGCATCTCTATCTTGTGTAGAGATCTTAAGGGAGCCATTCTTCTCCAGTAAGGTCTTACGTCTTATTGGAATTCTTTCATCCTTCAGGTTGCAGCAGGATATGAAATGCATAATTTTTGTCAATAGGATTATTACTGCAAGATCCTTGTCCTACATACTACAAAACCTTAAGAATCTAGCATCTTGGAAGTGCGATTTTCTTGTAGGAGTCCACTCGAAACTGAAGAGTATGTCAAGGAAGACGATGCAGATCATTCTTGATAAGTTTCGGTCTGGAGAGTTGAATCTATTGGTTGCAACTAAAGTTGGTGAAGAGGGACTAGATATTCAGACTTGCTGCCTTGTGATACGATTTGATCTTCCAGAAACTGTTGCTAGCTTTATACAGTCACGAGGTCGTGCACGGATGCCTCAGTCTGAATATATATTTTTGGTAAACAGTGGTAGTCAGAAAGAGTTGGATTTAATAGAAAACTTCAGGAAAGATGAAGAGCGAATGAACATGGAAGTTGCTTTCAGAACATCCAGTGAGACTTTTCTTAGTCCTGAGGAACGAACATATAAAGTTGCTTCTTCTGGAGCTTCCGTCACTTCTGGATATAGCATCTCATTGTTACACCAGTATTGTTCGAAGCTTCCACATGATGAGTATTATGTCCCCACTCCAGAATTCTATTTTCTTGGTGATTTGGAGGGAACTATTTGCCAGATAATTTTACCTTCCAATGCTCCAATGCATCAAATTGTCAGTACACCACAACCTTCGATGGAAGATGCCAAAAAAGATGCATGTCTAAAAGCCATTGAAGAATTGCATAAATTGGGTGCCTTAAGTGACTTTCTCTTGCCACAGCAAGACAATGCAAATGTGGAGGAGTCCATGCTAGACTCTTCTGATTATGACAGCATAGAAGATGAAGACTTACGAGAGGAACTACATGAAATGCTGGTTCCTGTTGTCCTAAAAGAGTCATGGAATAAGTTGGAGGATCTTGTCACCCTCAGCGCTTACTATTTTCAATTTGATCCTGAACCTAATGATAGAATCTATAGAAGTTTTGGTCTATTTGTCAAAGCACCTCTTCCAGCAGAGGCCAAAACAATGGAACTTGATCTTCATCTCGCTCATGGTAGATCTGTGATGACAAAGTTGGTGCCATCAGGATTTGCAGAATTTGTCAAAGATGAGATCTTGCTGGCTCAAAACTTTCAAGAAATGTTCCTCAAGCTCATTCTGGACCGATCAGAATTTGTTTCTGAATTTGTTCCCCTGGGAAACTATGATTTTAGCAGATCGAGCACATCAACCTTCTACCTATTGCTTCCAGTAACTTTAGGTGAAAATGATAAGATAAGCATAGATTGGAGGATTATCAAAAAATGTTTATCATCTCCAATATTCAGGGGTCCAGGAGATGCTGTGGACAGTAAAATTATATCTTCGGGTATTCGACTTGCATCTGGTTATAGAAGCATAGGTGAAGTTGAGAATAGTTTAGTTTATGTCCCATATAAGAAGACATTTTACTTCATCACTAATGTTACTCGAGAAAGAAATGCCTACAGCCAATATAAAGAAAAGGCAGATGCTTTAACCTATGTGGATCATCTAAGTAAAAAGTTTGACATTTCTCTTAAATACCCTGAGCAACGTCTTCTGTGTGCAAAACCAGTTTTCAATTTGCACAACTTGCTACACAATCGTAGGCAAGAAGATTCAGAAGCACAGCAACTAGATGAGTATTATATATATGTGCCTCCTGAGCTTTGCGAGCTGAAGGTAATTGGCTTTTCAAAAGATATTGGGAGTTCGGTATCTCTGTTACCATCAATCATGCACCGTCTGGAAAACTTGCTTGTCGCCATCGAACTGAAGCATGTATTATGCACTTCATTCCCCGAAGGAGCTGAGGTTACTTCGCAGAGGGTTCTAGAAGCCCTCACAACTGAAAAGTGCCAGGAGCGGATTTCCCTTGAAAGGCTTGAAATACTGGGAGATGCTTTCCTCAAATTTGCAGTTGGAAGGCACTTTTTCCTTTTGCATGCTTCACTTGATGAAGGCGAACTAACAAGGAAACGTTCAAATGTGGTAAACAATTCCAATTTATTGAAGCTAGCTACTAGAAGCAACTTGCAGGTATACATACGGGATCAGCCATTTGAACCCTCTCAGTTCTTCGCTTTAGGTCGCCCTTGCAAAAATATTTGTGACCAGGAGACAATAGGATCTCTTGATTCTCAAGATTTAAGCAGTGCAGTAAAACAGTCACATGCTCATCACGTCAGATGTAGTAAGGGTCACCAATGGTTACATAAGAAAACAATTGCTGATGTTGTTGAAGCCCTTGTTGGAGCATTCATAGTTGACAGCGGTTTTAAAGCGGCAACTGCATTTCTCAGATGGATTGGCATAAAAGTAAACTTTGAAGCATCAGAAGTTACAAAAGTATGCATAGCAAGCCGCAGATACGTTTCACTTGCTGCTTGCATAGACATTGCGGCCCTTGAAAATTCATTAGGGTATCAGTTTCTCCACAGAGGTTTGCTGTTACAGGCATTTGTACATCCTTCTTACAACAAACATGGAGGAGGTTGCTATCAGAGATTGGAGTTTCTTGGAGATGCTGTCTTGGATTACTTGATCACCTCATACCTGTATTCAGTGTATCCAAAGTTAAAGCCTGGCCATTTGACAGATTTGAGATCAATTGCTGTGAATAATAAAGCCTTTGCTACTGTTGCAGTAGCTCGATCCTTCCACAAGTTTCTACTCTCTGACACCAGTACCCTTTCTGAGGCTATAAAGACTTATGTGAACTTCATTGAGACATCTGCATCAGATAGTAGTCTGGTTGATGGGCCGAAATGCCCCAAGGCTCTGGGCGACTTGGTAGAGTCTTGTCTTGGTGCAATTCTTCTTGATACAGGGTTCGACTTAAACCTTGTTTGGAAGATAATGCTATCCTTTCTGGAGCCTGTCATGAGCTTTTCCAACATTCAGCTTAGTCCTGTTAGGGAACTACGGGAACTTTGCCAAGCTCATGCATGGAATCTTAAGTTTTTACCATCAAAGAAGGGTAAAACATTTTCAGTTGAGGCAACGGTGAAAGGAAATAATGTTTCTGCAATTGCTTCCTCAACTGCCTTGAACAAAAGGGAGGCTATTAGAATTTCTGCTCAGTTAATTTTCGAAACGTTGAAGGCTCAAGGCAATATACCAAAGTCTAAGTTGTCTTTGGAGGAAGTCTTGAAGTCAAGCTGCAAGTTGGAACCCAAACTTATCGGATATGATGAAACACCAATAGATGTTGCAGCTCTTGATGTTATTGGATTTGATAAGTTGAATGTACAAGAGGCTTCTAGCAGCAGTTCCAACTCAGATGTGCATTCTATGAGTGAAGTCAGCAGTGGTAACTCCTCCTGCATAAAACGTTTACGACAACCCCCAGCTTCACTTGGAGAAGTCAAAATGGACAGCACTAATGCCTGTAACAGTCGTAGCTCTGATGTTATCTCACAAACCAGAGGTGTGCCACACAAATTAACAGCAAAATCTCGTTTGTATGAAACCTGTGTTGCTAACTACTGGGAAACCCCTGAATTTGAATGTTACAAAGAGGAAGGACCAGGCCACATAAAATCGTTCACTTTCAAGGTTACAGTGAAGATAGATGATGCTTCGGATACACTTTTGGAGGCCTTTGGAGAACCTCGTACGAGCAAGAAAGCAGCAGCAGAGCATGCAGCGGAAGGGGCCGTTTGGTACTTGGAAAAAAAAGGTTACATAAAGGAGAGTGATCAAACAGAGGAACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006351 GO:0006353 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009798 GO:0009888 GO:0009892 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097659 GO:0098542 GO:0098586 GO:0098727 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1650

Amino Acids

185.08

Weight (kDa)

6.03

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 42 - 205 7.8e-17 Type III restriction enzyme, res subunit
DEAD PF00270 43 - 205 7.1e-19 DEAD/DEAH box helicase
Helicase_C PF00271 399 - 512 9e-18 Helicase conserved C-terminal domain
Dicer_dimer PF03368 579 - 664 2.1e-23 Dicer dimerisation domain
PAZ PF02170 872 - 992 5.9e-09 PAZ domain
Ribonucleas_3_3 PF14622 1036 - 1094 5.5e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 1037 - 1169 4e-18 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1225 - 1356 7.9e-14 Ribonuclease-III-like
Ribonuclease_3 PF00636 1245 - 1354 3.6e-21 Ribonuclease III domain
DND1_DSRM PF14709 1560 - 1635 3e-12 double strand RNA binding domain from DEAD END PROTEIN 1
dsrm PF00035 1561 - 1630 3.1e-06 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 3259
AccB1I GGYRCC 2 cut(s) 1958, 2287
AccBSI CCGCTC 1 cut(s) 3097
AccI GTMKAC 1 cut(s) 3273
AccII CGCG 2 cut(s) 11, 237
AclI AACGTT 3 cut(s) 3211, 4328, 4564
AclWI GGATC 9 cut(s) 101, 114, 1269, 2130, 2153, 2745, 3291, 3367, 3879
AcoI YGGCCR 1 cut(s) 3817
AcuI CTGAAG 5 cut(s) 1201, 1376, 1594, 2930, 3026
AcyI GRCGYC 1 cut(s) 431
AfaI GTAC 8 cut(s) 889, 1111, 1885, 3696, 3919, 4482, 4849, 4898
AfeI AGCGCT 1 cut(s) 2140
AfiI CCNNNNNNNGG 5 cut(s) 23, 391, 620, 3111, 3928
AflII CTTAAG 4 cut(s) 1159, 1301, 1963, 4187
AjiI CACGTC 1 cut(s) 3415
AjnI CCWGG 7 cut(s) 110, 2403, 2542, 3090, 3345, 3814, 4759
AjuI GAANNNNNNNTTGG 2 cut(s) 3465, 3497
Alw21I GWGCWC 3 cut(s) 91, 1537, 2435
Alw26I GTCTC 3 cut(s) 1658, 3344, 3953
Alw44I GTGCAC 1 cut(s) 1533
AlwI GGATC 9 cut(s) 101, 114, 1269, 2130, 2153, 2745, 3291, 3367, 3879
AlwNI CAGNNNCTG 2 cut(s) 795, 1502
Ama87I CYCGRG 1 cut(s) 2679
Aor51HI AGCGCT 1 cut(s) 2140
AoxI GGCC 9 cut(s) 95, 946, 2226, 3633, 3817, 3992, 4762, 4830, 4887
ApaLI GTGCAC 1 cut(s) 1533
AseI ATTAAT 1 cut(s) 1013
Asp700I GAANNNNTTC 2 cut(s) 1207, 2397
AspLEI GCGC 6 cut(s) 13, 237, 283, 295, 632, 2141
AspS9I GGNCC 9 cut(s) 26, 348, 767, 2371, 2540, 3634, 3992, 4757, 4887
AsuC2I CCSGG 1 cut(s) 124
AsuII TTCGAA 2 cut(s) 1763, 4323
AsuNHI GCTAGC 2 cut(s) 1508, 3247
AvaI CYCGRG 1 cut(s) 2679
AvaII GGWCC 6 cut(s) 26, 348, 767, 2371, 2540, 4757
AxyI CCTNAGG 1 cut(s) 1680
BaeGI GKGCMC 1 cut(s) 1537
BaeI ACNNNNGTAYC 2 cut(s) 3901, 3934
BalI TGGCCA 1 cut(s) 3819
BamHI GGATCC 1 cut(s) 106
BanI GGYRCC 2 cut(s) 1958, 2287
BanII GRGCYC 1 cut(s) 91
BarI GAAGNNNNNNTAC 4 cut(s) 616, 648, 2642, 2674
BauI CACGAG 1 cut(s) 1524
BbsI GAAGAC 6 cut(s) 714, 866, 1379, 2058, 2657, 2780
Bbv12I GWGCWC 3 cut(s) 91, 1537, 2435
BbvCI CCTCAGC 2 cut(s) 2135, 3042
BceAI ACGGC 1 cut(s) 4874
BciT130I CCWGG 7 cut(s) 112, 2405, 2544, 3092, 3347, 3816, 4761
BciVI GTATCC 2 cut(s) 3810, 4810
BclI TGATCA 2 cut(s) 3774, 4930
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 3 cut(s) 1658, 3344, 3953
BfmI CTRYAG 3 cut(s) 38, 2182, 2695
BfoI RGCGCY 3 cut(s) 296, 633, 2142
BfrI CTTAAG 4 cut(s) 1159, 1301, 1963, 4187
BfuAI ACCTGC 1 cut(s) 3259
BfuI GTATCC 2 cut(s) 3810, 4810
BglII AGATCT 3 cut(s) 1155, 2267, 2322
Bme1390I CCNGG 8 cut(s) 112, 124, 2405, 2544, 3092, 3347, 3816, 4761
Bme18I GGWCC 6 cut(s) 26, 348, 767, 2371, 2540, 4757
BmeT110I CYCGRG 1 cut(s) 2679
BmgBI CACGTC 1 cut(s) 3415
BmgT120I GGNCC 9 cut(s) 26, 348, 767, 2371, 2540, 3634, 3992, 4757, 4887
BmrFI CCNGG 8 cut(s) 112, 124, 2405, 2544, 3092, 3347, 3816, 4761
BmrI ACTGGG 3 cut(s) 400, 3134, 4729
BmtI GCTAGC 2 cut(s) 1512, 3251
BmuI ACTGGG 3 cut(s) 400, 3134, 4729
BpiI GAAGAC 6 cut(s) 714, 866, 1379, 2058, 2657, 2780
BpmI CTGGAG 6 cut(s) 597, 1162, 1428, 1731, 1782, 4124
Bpu10I CCTNAGC 6 cut(s) 690, 1089, 2135, 2778, 2895, 3042
Bpu14I TTCGAA 2 cut(s) 1763, 4323
BpuEI CTTGAG 4 cut(s) 323, 2342, 3357, 4323
BpuMI CCSGG 1 cut(s) 124
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 2 cut(s) 1382, 2250
BsaHI GRCGYC 1 cut(s) 431
BsaJI CCNNGG 5 cut(s) 92, 110, 2403, 2404, 4005
Bsc4I CCNNNNNNNGG 5 cut(s) 23, 391, 620, 3111, 3928
Bse21I CCTNAGG 1 cut(s) 1680
Bse3DI GCAATG 2 cut(s) 488, 3627
Bse8I GATNNNNATC 2 cut(s) 1382, 2250
BseBI CCWGG 7 cut(s) 112, 2405, 2544, 3092, 3347, 3816, 4761
BseDI CCNNGG 5 cut(s) 92, 110, 2403, 2404, 4005
BseGI GGATG 6 cut(s) 535, 785, 1211, 1545, 1655, 3697
BseJI GATNNNNATC 2 cut(s) 1382, 2250
BseLI CCNNNNNNNGG 5 cut(s) 23, 391, 620, 3111, 3928
BseMI GCAATG 2 cut(s) 488, 3627
BseRI GAGGAG 4 cut(s) 71, 2021, 3735, 4540
BseSI GKGCMC 1 cut(s) 1537
BseYI CCCAGC 1 cut(s) 4579
BsgI GTGCAG 1 cut(s) 3408
Bsh1236I CGCG 2 cut(s) 11, 237
BshFI GGCC 9 cut(s) 97, 948, 2228, 3635, 3819, 3994, 4764, 4832, 4889
BshNI GGYRCC 2 cut(s) 1958, 2287
BsiHKAI GWGCWC 3 cut(s) 91, 1537, 2435
BsiHKCI CYCGRG 1 cut(s) 2679
BsiSI CCGG 2 cut(s) 98, 124
BsiWI CGTACG 1 cut(s) 4847
BslFI GGGAC 4 cut(s) 361, 1464, 1775, 2624
BslI CCNNNNNNNGG 5 cut(s) 23, 391, 620, 3111, 3928
BsmAI GTCTC 3 cut(s) 1658, 3344, 3953
BsmFI GGGAC 4 cut(s) 361, 1464, 1775, 2624
BsmI GAATGC 2 cut(s) 3488, 4519
BsnI GGCC 9 cut(s) 97, 948, 2228, 3635, 3819, 3994, 4764, 4832, 4889
BsoBI CYCGRG 1 cut(s) 2679
Bsp119I TTCGAA 2 cut(s) 1763, 4323
Bsp1286I GDGCHC 3 cut(s) 91, 1537, 2435
Bsp1407I TGTACA 2 cut(s) 3694, 4480
BspANI GGCC 9 cut(s) 97, 948, 2228, 3635, 3819, 3994, 4764, 4832, 4889
BspFNI CGCG 2 cut(s) 11, 237
BspHI TCATGA 2 cut(s) 426, 4113
BspMAI CTGCAG 1 cut(s) 42
BspMI ACCTGC 1 cut(s) 3259
BspOI GCTAGC 2 cut(s) 1512, 3251
BspPI GGATC 9 cut(s) 101, 114, 1269, 2130, 2153, 2745, 3291, 3367, 3879
BspQI GCTCTTC 1 cut(s) 1617
BspT104I TTCGAA 2 cut(s) 1763, 4323
BspT107I GGYRCC 2 cut(s) 1958, 2287
BspTI CTTAAG 4 cut(s) 1159, 1301, 1963, 4187
BsrBI CCGCTC 1 cut(s) 3097
BsrDI GCAATG 2 cut(s) 488, 3627
BsrGI TGTACA 2 cut(s) 3694, 4480
BssECI CCNNGG 5 cut(s) 92, 110, 2403, 2404, 4005
BssNAI GTATAC 1 cut(s) 3274
BssNI GRCGYC 1 cut(s) 431
BssSI CACGAG 1 cut(s) 1524
BssT1I CCWWGG 1 cut(s) 4005
Bst1107I GTATAC 1 cut(s) 3274
Bst2BI CACGAG 1 cut(s) 1524
Bst2UI CCWGG 7 cut(s) 112, 2405, 2544, 3092, 3347, 3816, 4761
Bst6I CTCTTC 7 cut(s) 48, 156, 1352, 1440, 1617, 2029, 2220
BstACI GRCGYC 1 cut(s) 431
BstAFI CTTAAG 4 cut(s) 1159, 1301, 1963, 4187
BstAPI GCANNNNNTGC 2 cut(s) 2824, 4874
BstAUI TGTACA 2 cut(s) 3694, 4480
BstBAI YACGTR 1 cut(s) 449
BstBI TTCGAA 2 cut(s) 1763, 4323
BstC8I GCNNGC 9 cut(s) 309, 1510, 2331, 2906, 3177, 3249, 3592, 3619, 4875
BstEII GGTNACC 1 cut(s) 3431
BstENI CCTNNNNNAGG 1 cut(s) 3926
BstF5I GGATG 6 cut(s) 535, 785, 1211, 1545, 1655, 3697
BstFNI CGCG 2 cut(s) 11, 237
BstH2I RGCGCY 3 cut(s) 296, 633, 2142
BstHHI GCGC 6 cut(s) 13, 237, 283, 295, 632, 2141
BstMAI GTCTC 3 cut(s) 1658, 3344, 3953
BstNI CCWGG 7 cut(s) 112, 2405, 2544, 3092, 3347, 3816, 4761
BstNSI RCATGY 5 cut(s) 1926, 3014, 3179, 3407, 4877
BstPI GGTNACC 1 cut(s) 3431
BstSCI CCNGG 8 cut(s) 110, 122, 2403, 2542, 3090, 3345, 3814, 4759
BstSFI CTRYAG 3 cut(s) 38, 2182, 2695
BstSLI GKGCMC 1 cut(s) 1537
BstSNI TACGTA 1 cut(s) 449
BstUI CGCG 2 cut(s) 11, 237
BstV2I GAAGAC 6 cut(s) 714, 866, 1379, 2058, 2657, 2780
BstX2I RGATCY 7 cut(s) 106, 1155, 1274, 2122, 2267, 2322, 3359
BstXI CCANNNNNNTGG 3 cut(s) 764, 2236, 4012
BstYI RGATCY 7 cut(s) 106, 1155, 1274, 2122, 2267, 2322, 3359
BstZ17I GTATAC 1 cut(s) 3274
Bsu36I CCTNAGG 1 cut(s) 1680
BsuI GTATCC 2 cut(s) 3810, 4810
BsuRI GGCC 9 cut(s) 97, 948, 2228, 3635, 3819, 3994, 4764, 4832, 4889
BtrI CACGTC 1 cut(s) 3415
BtsCI GGATG 6 cut(s) 535, 785, 1211, 1545, 1655, 3697
BtsI GCAGTG 3 cut(s) 219, 3391, 4546
BtsIMutI CAGTG 8 cut(s) 219, 842, 1579, 1666, 3391, 3801, 4546, 4800
BveI ACCTGC 1 cut(s) 3259
Cac8I GCNNGC 9 cut(s) 309, 1510, 2331, 2906, 3177, 3249, 3592, 3619, 4875
CaiI CAGNNNCTG 2 cut(s) 795, 1502
CciI TCATGA 2 cut(s) 426, 4113
CfoI GCGC 6 cut(s) 13, 237, 283, 295, 632, 2141
Cfr13I GGNCC 9 cut(s) 26, 348, 767, 2371, 2540, 3634, 3992, 4757, 4887
CseI GACGC 2 cut(s) 17, 439
Csp6I GTAC 8 cut(s) 888, 1110, 1884, 3695, 3918, 4481, 4848, 4897
CviQI GTAC 8 cut(s) 888, 1110, 1884, 3695, 3918, 4481, 4848, 4897
DraI TTTAAA 1 cut(s) 3511
EaeI YGGCCR 1 cut(s) 3817
Eam1104I CTCTTC 7 cut(s) 48, 156, 1352, 1440, 1617, 2029, 2220
EarI CTCTTC 7 cut(s) 48, 156, 1352, 1440, 1617, 2029, 2220
Ecl136II GAGCTC 1 cut(s) 89
Eco105I TACGTA 1 cut(s) 449
Eco130I CCWWGG 1 cut(s) 4005
Eco147I AGGCCT 1 cut(s) 4832
Eco24I GRGCYC 1 cut(s) 91
Eco47I GGWCC 6 cut(s) 26, 348, 767, 2371, 2540, 4757
Eco47III AGCGCT 1 cut(s) 2140
Eco53kI GAGCTC 1 cut(s) 89
Eco57I CTGAAG 5 cut(s) 1201, 1376, 1594, 2930, 3026
Eco81I CCTNAGG 1 cut(s) 1680
Eco88I CYCGRG 1 cut(s) 2679
Eco91I GGTNACC 1 cut(s) 3431
EcoICRI GAGCTC 1 cut(s) 89
EcoNI CCTNNNNNAGG 1 cut(s) 3926
EcoO109I RGGNCCY 2 cut(s) 26, 348
EcoO65I GGTNACC 1 cut(s) 3431
EcoRI GAATTC 2 cut(s) 1203, 1802
EcoRII CCWGG 7 cut(s) 110, 2403, 2542, 3090, 3345, 3814, 4759
EcoT14I CCWWGG 1 cut(s) 4005
EcoT22I ATGCAT 5 cut(s) 1244, 1872, 1924, 3587, 4180
EcoT38I GRGCYC 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 4005
FalI AAGNNNNNCTT 4 cut(s) 315, 347, 2199, 2231
FaqI GGGAC 4 cut(s) 361, 1464, 1775, 2624
FauNDI CATATG 1 cut(s) 266
FbaI TGATCA 2 cut(s) 3774, 4930
FblI GTMKAC 1 cut(s) 3273
FokI GGATG 6 cut(s) 522, 772, 1198, 1552, 1642, 3684
FriOI GRGCYC 1 cut(s) 91
GlaI GCGC 6 cut(s) 12, 236, 282, 294, 631, 2140
GsaI CCCAGC 1 cut(s) 4583
GsuI CTGGAG 6 cut(s) 597, 1162, 1428, 1731, 1782, 4124
HaeII RGCGCY 3 cut(s) 296, 633, 2142
HaeIII GGCC 9 cut(s) 97, 948, 2228, 3635, 3819, 3994, 4764, 4832, 4889
HapII CCGG 2 cut(s) 98, 124
HgaI GACGC 2 cut(s) 17, 439
HhaI GCGC 6 cut(s) 13, 237, 283, 295, 632, 2141
Hin1I GRCGYC 1 cut(s) 431
Hin6I GCGC 6 cut(s) 11, 235, 281, 293, 630, 2139
HinP1I GCGC 6 cut(s) 11, 235, 281, 293, 630, 2139
HincII GTYRAC 1 cut(s) 3499
HindII GTYRAC 1 cut(s) 3499
HindIII AAGCTT 1 cut(s) 1766
HpaII CCGG 2 cut(s) 98, 124
Hpy99I CGWCG 1 cut(s) 11
HpyCH4IV ACGT 8 cut(s) 448, 1192, 2785, 3211, 3414, 3603, 4328, 4564
HpySE526I ACGT 8 cut(s) 448, 1192, 2785, 3211, 3414, 3603, 4328, 4564
Hsp92I GRCGYC 1 cut(s) 431
HspAI GCGC 6 cut(s) 11, 235, 281, 293, 630, 2139
Ksp22I TGATCA 2 cut(s) 3774, 4930
LguI GCTCTTC 1 cut(s) 1617
MaeII ACGT 8 cut(s) 448, 1192, 2785, 3211, 3414, 3603, 4328, 4564
MbiI CCGCTC 1 cut(s) 3097
MfeI CAATTG 4 cut(s) 134, 3456, 3840, 4260
MflI RGATCY 7 cut(s) 106, 1155, 1274, 2122, 2267, 2322, 3359
MhlI GDGCHC 3 cut(s) 91, 1537, 2435
MlsI TGGCCA 1 cut(s) 3819
MluNI TGGCCA 1 cut(s) 3819
MlyI GAGTC 6 cut(s) 83, 1350, 2015, 2018, 2111, 4037
MmeI TCCRAC 7 cut(s) 1572, 2097, 3133, 3463, 4149, 4380, 4530
Mox20I TGGCCA 1 cut(s) 3819
Mph1103I ATGCAT 5 cut(s) 1244, 1872, 1924, 3587, 4180
MroXI GAANNNNTTC 2 cut(s) 1207, 2397
MscI TGGCCA 1 cut(s) 3819
MslI CAYNNNNRTG 5 cut(s) 173, 212, 269, 540, 762
Msp20I TGGCCA 1 cut(s) 3819
MspA1I CMGCKG 4 cut(s) 37, 826, 3504, 4880
MspCI CTTAAG 4 cut(s) 1159, 1301, 1963, 4187
MspI CCGG 2 cut(s) 98, 124
MspR9I CCNGG 8 cut(s) 112, 124, 2405, 2544, 3092, 3347, 3816, 4761
MunI CAATTG 4 cut(s) 134, 3456, 3840, 4260
Mva1269I GAATGC 2 cut(s) 3488, 4519
MvaI CCWGG 7 cut(s) 112, 2405, 2544, 3092, 3347, 3816, 4761
MvnI CGCG 2 cut(s) 11, 237
NciI CCSGG 1 cut(s) 124
NdeI CATATG 1 cut(s) 266
NheI GCTAGC 2 cut(s) 1508, 3247
NmuCI GTSAC 8 cut(s) 866, 1521, 1720, 1967, 2128, 3341, 3399, 3431
NsiI ATGCAT 5 cut(s) 1244, 1872, 1924, 3587, 4180
NspI RCATGY 5 cut(s) 1926, 3014, 3179, 3407, 4877
NspV TTCGAA 2 cut(s) 1763, 4323
PaeI GCATGC 2 cut(s) 3179, 4877
PaeR7I CTCGAG 1 cut(s) 2679
PagI TCATGA 2 cut(s) 426, 4113
PasI CCCWGGG 1 cut(s) 2404
PceI AGGCCT 1 cut(s) 4832
PciSI GCTCTTC 1 cut(s) 1617
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 2 cut(s) 3488, 4519
PdmI GAANNNNTTC 2 cut(s) 1207, 2397
PfeI GAWTC 8 cut(s) 326, 1064, 1306, 1417, 2178, 2849, 3368, 4183
Pfl23II CGTACG 1 cut(s) 4847
PfoI TCCNGGA 1 cut(s) 2542
PleI GAGTC 6 cut(s) 82, 1349, 2015, 2017, 2110, 4036
PpsI GAGTC 6 cut(s) 82, 1349, 2015, 2017, 2110, 4036
Ppu21I YACGTR 1 cut(s) 449
PpuMI RGGWCCY 2 cut(s) 26, 348
PshBI ATTAAT 1 cut(s) 1013
Psp124BI GAGCTC 1 cut(s) 91
Psp1406I AACGTT 3 cut(s) 3211, 4328, 4564
Psp5II RGGWCCY 2 cut(s) 26, 348
Psp6I CCWGG 7 cut(s) 110, 2403, 2542, 3090, 3345, 3814, 4759
PspEI GGTNACC 1 cut(s) 3431
PspFI CCCAGC 1 cut(s) 4579
PspGI CCWGG 7 cut(s) 110, 2403, 2542, 3090, 3345, 3814, 4759
PspLI CGTACG 1 cut(s) 4847
PspPI GGNCC 9 cut(s) 26, 348, 767, 2371, 2540, 3634, 3992, 4757, 4887
PspPPI RGGWCCY 2 cut(s) 26, 348
PstI CTGCAG 1 cut(s) 42
PstNI CAGNNNCTG 2 cut(s) 795, 1502
PsuI RGATCY 7 cut(s) 106, 1155, 1274, 2122, 2267, 2322, 3359
PvuII CAGCTG 1 cut(s) 826
RsaI GTAC 8 cut(s) 889, 1111, 1885, 3696, 3919, 4482, 4849, 4898
RsaNI GTAC 8 cut(s) 888, 1110, 1884, 3695, 3918, 4481, 4848, 4897
RseI CAYNNNNRTG 5 cut(s) 173, 212, 269, 540, 762
SacI GAGCTC 1 cut(s) 91
SapI GCTCTTC 1 cut(s) 1617
Sau96I GGNCC 9 cut(s) 26, 348, 767, 2371, 2540, 3634, 3992, 4757, 4887
SchI GAGTC 6 cut(s) 83, 1350, 2015, 2018, 2111, 4037
ScrFI CCNGG 8 cut(s) 112, 124, 2405, 2544, 3092, 3347, 3816, 4761
SduI GDGCHC 3 cut(s) 91, 1537, 2435
SfcI CTRYAG 3 cut(s) 38, 2182, 2695
Sfr274I CTCGAG 1 cut(s) 2679
SfuI TTCGAA 2 cut(s) 1763, 4323
SinI GGWCC 6 cut(s) 26, 348, 767, 2371, 2540, 4757
SlaI CTCGAG 1 cut(s) 2679
SmiMI CAYNNNNRTG 5 cut(s) 173, 212, 269, 540, 762
SmlI CTYRAG 9 cut(s) 338, 1159, 1301, 1963, 2357, 2679, 3372, 4187, 4338
SmoI CTYRAG 9 cut(s) 338, 1159, 1301, 1963, 2357, 2679, 3372, 4187, 4338
SnaBI TACGTA 1 cut(s) 449
SphI GCATGC 2 cut(s) 3179, 4877
SseBI AGGCCT 1 cut(s) 4832
SspI AATATT 3 cut(s) 927, 2532, 3337
SstI GAGCTC 1 cut(s) 91
StuI AGGCCT 1 cut(s) 4832
StyD4I CCNGG 8 cut(s) 110, 122, 2403, 2542, 3090, 3345, 3814, 4759
StyI CCWWGG 1 cut(s) 4005
TaiI ACGT 8 cut(s) 451, 1195, 2788, 3214, 3417, 3606, 4331, 4567
TaqII GACCGA 2 cut(s) 1392, 2388
TatI WGTACW 4 cut(s) 887, 1883, 3694, 4480
TauI GCSGC 4 cut(s) 16, 3518, 3597, 3635
TfiI GAWTC 8 cut(s) 326, 1064, 1306, 1417, 2178, 2849, 3368, 4183
TscAI CASTG 8 cut(s) 219, 842, 1579, 1666, 3391, 3801, 4546, 4800
TseFI GTSAC 8 cut(s) 866, 1521, 1720, 1967, 2128, 3341, 3399, 3431
Tsp45I GTSAC 8 cut(s) 866, 1521, 1720, 1967, 2128, 3341, 3399, 3431
TspGWI ACGGA 2 cut(s) 1552, 1708
TspRI CASTG 8 cut(s) 219, 842, 1579, 1666, 3391, 3801, 4546, 4800
Vha464I CTTAAG 4 cut(s) 1159, 1301, 1963, 4187
VneI GTGCAC 1 cut(s) 1533
VpaK11BI GGWCC 6 cut(s) 26, 348, 767, 2371, 2540, 4757
VspI ATTAAT 1 cut(s) 1013
XagI CCTNNNNNAGG 1 cut(s) 3926
XbaI TCTAGA 2 cut(s) 809, 3064
XceI RCATGY 5 cut(s) 1926, 3014, 3179, 3407, 4877
XhoI CTCGAG 1 cut(s) 2679
XmiI GTMKAC 1 cut(s) 3273
XmnI GAANNNNTTC 2 cut(s) 1207, 2397
Zsp2I ATGCAT 5 cut(s) 1244, 1872, 1924, 3587, 4180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.