Rw2G001490

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004795
Physical Location & Seq
Reverse (-)
1245557 .. 1258334
12778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G001490.1

Sequence Viewer

Length: 4953 bp
ATGGACGAAGTGCCGCCCACTACGGGTCCTCCACCGCCGCAGAGCGGTGGGGAAGAGGTGGGTCGGGCTGGGGAGTCGGGTGATGGAGCTCCGAGGCCGGATAAGGATCCGAGGAAGGTTGCCCGGAGGTATCAATTGGAGCTGTGTCAGAAAGCTGTGGAGGAGAACATAATCGTCTATTTGGAAACTGGCTGTGGAAAGACCCACATAGCAGTGTTGCTTATCTATGAGCTGCGTGAGTTGATAATGAAACCTCATAAGAACATATGTGTGTTTCTTGCGCCTACGGTGGCGCTTGTTCAACAGCAAGCAAGAGTTATAGAAGATTCCCTTGATCTCAAGGTTGGGACCTATTGTGGAAGCTCTAAGAAATTGAAAACTCACCAAGACTGGGAAAAAGAGATGGAACAATATGAGGTTTTTGTCATGACGCCTCAAATACTGTTACGTAACTTATACCACCGTATTATTAAGATGGAGTTGATTGCATTGCTGATTTTTGATGAGTGTCATCACGCTCAAATAAAAAGCAACCATCCTTATGCAGAAATTATGAAATTTTACAAGAATGATGCCACGAAACTTCCTCGTATATTTGGCATGACTGCATCTCCAGTTGTAGGTAAAGGCACTTCTAGTCAATCGAATTTATCAAAGAGTATCAATAGTCTTGAAAATTTACTTGATGCTAAGGTTTATACAGTTGAAGACAAGACAGAATTGCATTGTTCTGTTGTTTCTCCTGTTATCAACGTCCATCGTTATGGTCCTGTTATTAGTGGCACATCCAGCCCCTGCATGCCTTTTTCTAGAAAACTTGACCTGCTGAAACGCCAGTGTATAGCGGAACTTAGTAAGAAGACGAGTGACTTCCAAAGTCTAAGGAGTACAAAGAAAACATTAAACAGAGTGCATGACAGTATAATATTTTGCTTGGAAACTCTTGGCCTCTGGGGTGCGTTACAAGCTAGGCATATTCTTTCCAGTGGTGATAACTTTGAACGAAATGAATTAATAGAGGAAGGCAATAATGATATAGAGGCAGAAGGAAATAATAGTGATGACTCTGTATGTGCGGAATATCTGGCTCAGGCAGCTGACATCATTCGTACTGACTATGTGAAAGATGCTGTTGCATCTGGTCTATCTCGTGTAGAGATCTTAAAGGAGCCATTCTTCTCAAGTAAGGTCTTACGTCTTATTGGAATTCTTTCATCCTTCAGGTTGCAGCAGGATATGAAATGCATAATTTTTGTCAATAGGATTATTACTGCAAGATCCTTGTCCTACATTCTACAAAACCTTAAGAATCTAGCATCTTGGAAGTGCGATTTTCTTGTAGGAGTCCACTCGAAACTGAAGAGTATGTCAAGGAAGACGATGCAGATCATTCTTGATAAGTTTCGGTCTGGAGAGTTGAATCTATTGGTTGCAACTAAAGTTGGTGAAGAGGGACTAGATATTCAGACTTGCTGCCTCGTGATACGATTTGATCTTCCAGAAACTGTTGCTAGCTTTATACAGTCACGAGGTCGTGCACGGATGCCTCAGTCTGAATATATATTTTTGGTAAACAGTGGTAGTCAGAAAGAGTTGGATTTAATAGAAAACTTCAGGAAAGATGAAGAGCGAATGAACATGGAAGTTGCTTTCAGAACATCCAGTGAGACTTTTCTTAGTCCTGAGGAACGAACATATAAAGTTGCTTCTTCTGGAGCTTCCGTCACTTCTGGATATAGCATCTCATTGTTACACCTGTATTGTTCGAAGCTTCCACATGATGAGTATTATGTCCCCACTCCGGAATTCTATTTTCTTGGTGATTTGGAGGGAACTATTTGCCATATAATTTTACCTTCCAATGCTCCAATGCATCAAATTGTCAGTACACCACAATCTTCGATGGAAGATGCCAAAAAAGATGCATGTCTAAAAGCCATCGAAGAATTGCATAAATTGGGTGCCTTAAGTGACTATCTCTTGCCACAGCAAGACAATGCAAATGTGGAGGAGTCCATGCTAGACTCTTCTGATTATGACAGCATGGAAGATGAAGACTTACGAGAGGAACTACATGAAATGCTGGTTCCTGCTGTCCTAAAAGAGTCATGGAATAAGTTGGAGGATCTCGTCACCCTCAGCGCTTACTATTTTCAATTTGATCCTGAACCTAATGATAGAATCTATAGAAGTTTTGGTCTATTTGTCAAAGCACCTCTTCCAGCAGAGGCCGAAACAATGGAACTTGATCTTCACCTCGCTCATGGTAGATCTGTGATGACAAAGTTGGTGCCATCAGGATTTGCAGAATTTGTCAAAGATGAGATCTTGCTGGCTCAAAACTTTCAAGAAATGTTCCTCAAGCTCATTCTGGACCGATCAGAATTTGTTTCTGAATTTGTTCCCCTGGGAAAATATGATTTTAGCAGATCGAGCACATCAACCTTCTACCTATTGCTTCCAGTAACTTTGGGTGAAAATGATAAGATAAACATAGATTGGAGGATTATCAAAAAATGTTTATCATCTCCAATATTCAGGGGTCCAGGAGATGCTATGGACAGTAAAATTATATCTTCGGGTATTCGACTTGCATCTGGTTATAGAAGCATCAGTGAAGTTGAGGATAGTTTAGTTTATGTCCCATATAAGAATACATTTTACTTCATCACTAATGTTACTCGCGAAAGAAATGCCTACAGCCAATATAAAGAAAAGTCAGATGCTTTAACCTATGTGGATCATCTAAGTAAAAAGTTTGACATTTATCTTAAATACCCTGAGCAACATCTTTTGTGTGCAAAACCAGTTTTCAATTTGCACAACTTGCTCCACAATCGTAGGCAAGAAGATTCAGAAGCACAGCAACTAGATGAGTATTATATATATGTGCCTCCTGAGCTTTGCGAGCTGAAGGTAATTGGCTTTTCAAAAGATATTGGGAGTTCGGTATCTCTGTTACCATCAATCATGCACCGTCTGGAAAACTTGCTTGTCGCCATCGAACTGAAGCATGTATTACGCACTTCATTCCCCGAAGGAGCTGAGGTTACTTCGCAGAGAGTTCTAGAAGCCCTCACAACTGAGAAGTGCCAGGAGCGGATTTCCCTTGAAAGGCTTGAAATACTAGGAGATGCTTTCCTCAAATTTGCAGTTGGAAGGCACTTCTTCCTTTTGCATGCTTCACTTGATGAAGGTGAACTAACAAGGAAACGTTCAAATGTGGTAAACAATTCCAATTTATTGAAGCTAGCTACTAGAAGCAACTTACAGGTATACATACGGGATCAGCCATTTGAACCCTCTCAGTTCTTCACTTTAGGCCGCCCTTGCAAAAATATTTGTGACCAGGAGACTATAGGATCTCTTGATTCTCAAGATTTAGGCAGTGCAGTAAAACAGTCACATGCTCGTCACGTCAGATGTAGTAAGGGTCACCATTGGTTACATAAGAAAACAATTGCTGATGTTGTTGAAGCCCTTGTTGGAGCATTCATAGTTGACAGCGGTTTTAAAGCGGCAACTGCATTTCTCAGATGGATTGGCATAAAAGTAAACTTTGAAGCATCAGAAGTTACTAAAGTATGCATAGCAAGCAGCAGATACATTTCACTTGCTGCTTGCATAGACATTGCGGCCCTTGAAAATTCATTAGGGTATCAGTTTCTCCACAGAGGTTTGCTGTTACAGGCATTTGTACATCCTTCTTACAACAAACATGGAGGAGGTTGCTATCAGAGATTGGAGTTTCTTGGAGATGCTGTCTTGGATTACTTGATCACCTCATACCTGTATTCAGTGTATCCAAAGTTAAAGCCTGGCCATTTGACAGATTTGAGATCAATTGCTGTGAATAATAAAGCCTTTGCTACTGTTGCAGTAGCTCGATCCTTCCACAAGTTTCTACTCTCTGACACCAGTACCCTTTCTGAGGCTATAAAGACTTATGTGAACTTCATTGAGACATCTGCATCAGATAGCAGTCTGGTTGATGGGCCAAAATGCCCCAAGGCTCTGGGCGACTTGGTAGAGTCTTGTCTTGGTGCAATTCTTCTTGATACAGGGTTCGACTTAAACCTTGTTTGGAAGATAATGCTATCCTTTCTGGAGCCTGTCATGAGCTTTTCCAACATTCAGCTTAGTCCTGTTAGGGAACTACGGGAACTTTGCCAAGCTCATGCATGGGTTCTTAAGTTTTTACCATCAAAGAAGGGTAAAACATTTTCAGTTGAGGCAACAGTGAATGGGAATAATGTTTCTGCAACTGCTTCCTCAACTGCCCTGAACAAAAGGGAGGCTATTAGAATTTCGGCTCAGTTAATTTTCGAAACGTTGAAGGCTCAAGGCAATATTCCAAAGTCTAAGTTGTCTTTGGAGGAAGTCTTGAAGTCAAGCTGCAAGTTGGAACCCAAACTTATCGGATATGATGAAACACCAATAGATGTTACAGCTCTTGATGTTATTGGATTTGATAAGTTGAATGTACAAGAGGCTTCTAGCAGCAGTTCCAACTCAGATGTGCATTCTATGAGTGAAGTCAGCAGTGGTAACTTCTCCTGCATAAAACGTTTACGACAACCCCCAGCTTCACTTGGAGAAGTCAAAATGGACAGCACTAATGCCTGTAACAGTTGTAGCTCTGATGCTATCTCACAAACCAGAGGTGTGCCACACAAATTAACAGCAAAATCTCGTTTGCATGAGACCTGTGTTGCTAACTTCTGGGAACCCCCTGAATTTGAATGTTGCAAAGAGGAAGGGCCAGGCCACATAAAATTGTTCACTTTCAAGGTTACAGTGAAGATAGATGATGTTTCGGATACACTTTTGGAGGCCTTTGGAGAACCTCGTACGAGCAAGAAAGCAGCAGCAGAGCATGCAGCGGAAGGGGCACTTTGGTACTTGGAAAAGAAAGGTTACATAGAGGAGAGTGATCAATCAGAGGAACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006351 GO:0006353 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009798 GO:0009888 GO:0009892 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097659 GO:0098542 GO:0098586 GO:0098727 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1650

Amino Acids

185.08

Weight (kDa)

5.91

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 42 - 205 3.1e-17 Type III restriction enzyme, res subunit
DEAD PF00270 43 - 205 1.7e-18 DEAD/DEAH box helicase
Helicase_C PF00271 400 - 513 9e-18 Helicase conserved C-terminal domain
Dicer_dimer PF03368 580 - 665 3.4e-23 Dicer dimerisation domain
PAZ PF02170 873 - 993 3.3e-09 PAZ domain
Ribonucleas_3_3 PF14622 1037 - 1095 5.2e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 1038 - 1170 3.3e-18 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1226 - 1357 7.9e-14 Ribonuclease-III-like
Ribonuclease_3 PF00636 1246 - 1355 3.6e-21 Ribonuclease III domain
DND1_DSRM PF14709 1561 - 1636 1.8e-14 double strand RNA binding domain from DEAD END PROTEIN 1
dsrm PF00035 1562 - 1632 2.8e-06 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 831
AccB1I GGYRCC 2 cut(s) 1961, 2290
AccBSI CCGCTC 2 cut(s) 45, 3100
AccI GTMKAC 1 cut(s) 3276
AccII CGCG 1 cut(s) 2685
AccIII TCCGGA 1 cut(s) 1801
AclI AACGTT 3 cut(s) 3214, 4331, 4567
AclWI GGATC 9 cut(s) 101, 114, 1272, 2133, 2156, 2748, 3294, 3370, 3882
AcoI YGGCCR 1 cut(s) 3820
AcuI CTGAAG 5 cut(s) 1204, 1379, 1597, 2933, 3029
AcyI GRCGYC 1 cut(s) 431
AfaI GTAC 8 cut(s) 889, 1111, 1888, 3699, 3922, 4485, 4852, 4901
AfeI AGCGCT 1 cut(s) 2143
AfiI CCNNNNNNNGG 7 cut(s) 23, 44, 391, 620, 1801, 3114, 3931
AflII CTTAAG 3 cut(s) 1304, 1966, 4190
AjiI CACGTC 1 cut(s) 3418
AjnI CCWGG 6 cut(s) 2406, 2545, 3093, 3348, 3817, 4762
AjuI GAANNNNNNNTTGG 2 cut(s) 3468, 3500
Alw21I GWGCWC 3 cut(s) 91, 1540, 2438
Alw26I GTCTC 4 cut(s) 1661, 3347, 3956, 4697
Alw44I GTGCAC 1 cut(s) 1536
AlwI GGATC 9 cut(s) 101, 114, 1272, 2133, 2156, 2748, 3294, 3370, 3882
AlwNI CAGNNNCTG 2 cut(s) 795, 1505
Aor13HI TCCGGA 1 cut(s) 1801
Aor51HI AGCGCT 1 cut(s) 2143
ApaLI GTGCAC 1 cut(s) 1536
AseI ATTAAT 1 cut(s) 1013
Asp700I GAANNNNTTC 2 cut(s) 1210, 2400
AspLEI GCGC 3 cut(s) 283, 295, 2144
AspS9I GGNCC 8 cut(s) 26, 348, 767, 2374, 2543, 3637, 3995, 4760
AsuC2I CCSGG 1 cut(s) 124
AsuII TTCGAA 2 cut(s) 1766, 4326
AsuNHI GCTAGC 2 cut(s) 1511, 3250
AvaII GGWCC 5 cut(s) 26, 348, 767, 2374, 2543
AxyI CCTNAGG 1 cut(s) 1683
BaeGI GKGCMC 2 cut(s) 1540, 4894
BaeI ACNNNNGTAYC 2 cut(s) 3904, 3937
BalI TGGCCA 1 cut(s) 3822
BamHI GGATCC 1 cut(s) 106
BanI GGYRCC 2 cut(s) 1961, 2290
BanII GRGCYC 1 cut(s) 91
BarI GAAGNNNNNNTAC 2 cut(s) 616, 648
BauI CACGAG 3 cut(s) 1149, 1478, 1527
BbsI GAAGAC 4 cut(s) 714, 866, 1382, 2061
Bbv12I GWGCWC 3 cut(s) 91, 1540, 2438
BbvCI CCTCAGC 2 cut(s) 2138, 3045
BcgI CGANNNNNNTGC 2 cut(s) 2675, 2709
BciT130I CCWGG 6 cut(s) 2408, 2547, 3095, 3350, 3819, 4764
BciVI GTATCC 2 cut(s) 3813, 4813
BclI TGATCA 2 cut(s) 3777, 4933
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 4 cut(s) 1661, 3347, 3956, 4697
BfmI CTRYAG 3 cut(s) 2185, 2698, 3357
BfoI RGCGCY 2 cut(s) 296, 2145
BfrI CTTAAG 3 cut(s) 1304, 1966, 4190
BfuAI ACCTGC 1 cut(s) 831
BfuI GTATCC 2 cut(s) 3813, 4813
BglII AGATCT 3 cut(s) 1158, 2270, 2325
Bme1390I CCNGG 7 cut(s) 124, 2408, 2547, 3095, 3350, 3819, 4764
Bme18I GGWCC 5 cut(s) 26, 348, 767, 2374, 2543
BmgBI CACGTC 1 cut(s) 3418
BmgT120I GGNCC 8 cut(s) 26, 348, 767, 2374, 2543, 3637, 3995, 4760
BmrFI CCNGG 7 cut(s) 124, 2408, 2547, 3095, 3350, 3819, 4764
BmrI ACTGGG 1 cut(s) 400
BmtI GCTAGC 2 cut(s) 1515, 3254
BmuI ACTGGG 1 cut(s) 400
BpiI GAAGAC 4 cut(s) 714, 866, 1382, 2061
BpmI CTGGAG 4 cut(s) 597, 1431, 1734, 4127
Bpu10I CCTNAGC 6 cut(s) 690, 1089, 2138, 2781, 2898, 3045
Bpu14I TTCGAA 2 cut(s) 1766, 4326
BpuEI CTTGAG 5 cut(s) 323, 1165, 2345, 3360, 4326
BpuMI CCSGG 1 cut(s) 124
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 2 cut(s) 105, 1385
BsaHI GRCGYC 1 cut(s) 431
BsaI GGTCTC 1 cut(s) 4697
BsaJI CCNNGG 5 cut(s) 92, 110, 2406, 2407, 4008
BsaWI WCCGGW 1 cut(s) 1801
Bsc4I CCNNNNNNNGG 7 cut(s) 23, 44, 391, 620, 1801, 3114, 3931
Bse1I ACTGG 9 cut(s) 193, 395, 614, 835, 984, 1662, 2462, 2807, 3918
Bse21I CCTNAGG 1 cut(s) 1683
Bse3DI GCAATG 2 cut(s) 488, 3630
Bse8I GATNNNNATC 2 cut(s) 105, 1385
BseAI TCCGGA 1 cut(s) 1801
BseBI CCWGG 6 cut(s) 2408, 2547, 3095, 3350, 3819, 4764
BseDI CCNNGG 5 cut(s) 92, 110, 2406, 2407, 4008
BseGI GGATG 6 cut(s) 535, 785, 1214, 1548, 1658, 3700
BseJI GATNNNNATC 2 cut(s) 105, 1385
BseLI CCNNNNNNNGG 7 cut(s) 23, 44, 391, 620, 1801, 3114, 3931
BseMI GCAATG 2 cut(s) 488, 3630
BseNI ACTGG 9 cut(s) 193, 395, 614, 835, 984, 1662, 2462, 2807, 3918
BseRI GAGGAG 4 cut(s) 176, 2024, 3738, 4940
BseSI GKGCMC 2 cut(s) 1540, 4894
BseYI CCCAGC 2 cut(s) 68, 4582
BsgI GTGCAG 1 cut(s) 3411
Bsh1236I CGCG 1 cut(s) 2685
BshNI GGYRCC 2 cut(s) 1961, 2290
BsiHKAI GWGCWC 3 cut(s) 91, 1540, 2438
BsiSI CCGG 3 cut(s) 98, 124, 1802
BsiWI CGTACG 1 cut(s) 4850
BslFI GGGAC 4 cut(s) 361, 1467, 1778, 2627
BslI CCNNNNNNNGG 7 cut(s) 23, 44, 391, 620, 1801, 3114, 3931
BsmAI GTCTC 4 cut(s) 1661, 3347, 3956, 4697
BsmFI GGGAC 4 cut(s) 361, 1467, 1778, 2627
BsmI GAATGC 2 cut(s) 3491, 4522
Bso31I GGTCTC 1 cut(s) 4697
Bsp119I TTCGAA 2 cut(s) 1766, 4326
Bsp1286I GDGCHC 4 cut(s) 91, 1540, 2438, 4894
Bsp13I TCCGGA 1 cut(s) 1801
Bsp1407I TGTACA 2 cut(s) 3697, 4483
Bsp68I TCGCGA 1 cut(s) 2685
BspEI TCCGGA 1 cut(s) 1801
BspFNI CGCG 1 cut(s) 2685
BspHI TCATGA 2 cut(s) 426, 4116
BspMI ACCTGC 1 cut(s) 831
BspOI GCTAGC 2 cut(s) 1515, 3254
BspPI GGATC 9 cut(s) 101, 114, 1272, 2133, 2156, 2748, 3294, 3370, 3882
BspQI GCTCTTC 1 cut(s) 1620
BspT104I TTCGAA 2 cut(s) 1766, 4326
BspT107I GGYRCC 2 cut(s) 1961, 2290
BspTI CTTAAG 3 cut(s) 1304, 1966, 4190
BspTNI GGTCTC 1 cut(s) 4697
BsrBI CCGCTC 2 cut(s) 45, 3100
BsrDI GCAATG 2 cut(s) 488, 3630
BsrGI TGTACA 2 cut(s) 3697, 4483
BsrI ACTGG 9 cut(s) 193, 395, 614, 835, 984, 1662, 2462, 2807, 3918
BssECI CCNNGG 5 cut(s) 92, 110, 2406, 2407, 4008
BssNAI GTATAC 1 cut(s) 3277
BssNI GRCGYC 1 cut(s) 431
BssSI CACGAG 3 cut(s) 1149, 1478, 1527
BssT1I CCWWGG 1 cut(s) 4008
Bst1107I GTATAC 1 cut(s) 3277
Bst2BI CACGAG 3 cut(s) 1149, 1478, 1527
Bst2UI CCWGG 6 cut(s) 2408, 2547, 3095, 3350, 3819, 4764
Bst6I CTCTTC 6 cut(s) 48, 1355, 1443, 1620, 2032, 2223
BstACI GRCGYC 1 cut(s) 431
BstAFI CTTAAG 3 cut(s) 1304, 1966, 4190
BstAPI GCANNNNNTGC 2 cut(s) 2827, 4877
BstAUI TGTACA 2 cut(s) 3697, 4483
BstBAI YACGTR 1 cut(s) 449
BstBI TTCGAA 2 cut(s) 1766, 4326
BstEII GGTNACC 1 cut(s) 3434
BstENI CCTNNNNNAGG 1 cut(s) 3929
BstF5I GGATG 6 cut(s) 535, 785, 1214, 1548, 1658, 3700
BstFNI CGCG 1 cut(s) 2685
BstH2I RGCGCY 2 cut(s) 296, 2145
BstHHI GCGC 3 cut(s) 283, 295, 2144
BstMAI GTCTC 4 cut(s) 1661, 3347, 3956, 4697
BstNI CCWGG 6 cut(s) 2408, 2547, 3095, 3350, 3819, 4764
BstNSI RCATGY 6 cut(s) 802, 1929, 3017, 3182, 3410, 4880
BstPI GGTNACC 1 cut(s) 3434
BstSCI CCNGG 7 cut(s) 122, 2406, 2545, 3093, 3348, 3817, 4762
BstSFI CTRYAG 3 cut(s) 2185, 2698, 3357
BstSLI GKGCMC 2 cut(s) 1540, 4894
BstSNI TACGTA 1 cut(s) 449
BstUI CGCG 1 cut(s) 2685
BstV2I GAAGAC 4 cut(s) 714, 866, 1382, 2061
BstX2I RGATCY 7 cut(s) 106, 1158, 1277, 2125, 2270, 2325, 3362
BstXI CCANNNNNNTGG 2 cut(s) 764, 4015
BstYI RGATCY 7 cut(s) 106, 1158, 1277, 2125, 2270, 2325, 3362
BstZ17I GTATAC 1 cut(s) 3277
Bsu36I CCTNAGG 1 cut(s) 1683
BsuI GTATCC 2 cut(s) 3813, 4813
BtrI CACGTC 1 cut(s) 3418
BtsCI GGATG 6 cut(s) 535, 785, 1214, 1548, 1658, 3700
BtsI GCAGTG 3 cut(s) 219, 3394, 4549
BtuMI TCGCGA 1 cut(s) 2685
BveI ACCTGC 1 cut(s) 831
CaiI CAGNNNCTG 2 cut(s) 795, 1505
CciI TCATGA 2 cut(s) 426, 4116
CfoI GCGC 3 cut(s) 283, 295, 2144
Cfr13I GGNCC 8 cut(s) 26, 348, 767, 2374, 2543, 3637, 3995, 4760
CseI GACGC 1 cut(s) 439
Csp6I GTAC 8 cut(s) 888, 1110, 1887, 3698, 3921, 4484, 4851, 4900
CviQI GTAC 8 cut(s) 888, 1110, 1887, 3698, 3921, 4484, 4851, 4900
DraI TTTAAA 1 cut(s) 3514
EaeI YGGCCR 1 cut(s) 3820
Eam1104I CTCTTC 6 cut(s) 48, 1355, 1443, 1620, 2032, 2223
EarI CTCTTC 6 cut(s) 48, 1355, 1443, 1620, 2032, 2223
Ecl136II GAGCTC 1 cut(s) 89
Eco105I TACGTA 1 cut(s) 449
Eco130I CCWWGG 1 cut(s) 4008
Eco147I AGGCCT 1 cut(s) 4835
Eco24I GRGCYC 1 cut(s) 91
Eco31I GGTCTC 1 cut(s) 4697
Eco47I GGWCC 5 cut(s) 26, 348, 767, 2374, 2543
Eco47III AGCGCT 1 cut(s) 2143
Eco53kI GAGCTC 1 cut(s) 89
Eco57I CTGAAG 5 cut(s) 1204, 1379, 1597, 2933, 3029
Eco81I CCTNAGG 1 cut(s) 1683
Eco91I GGTNACC 1 cut(s) 3434
EcoICRI GAGCTC 1 cut(s) 89
EcoNI CCTNNNNNAGG 1 cut(s) 3929
EcoO109I RGGNCCY 2 cut(s) 26, 348
EcoO65I GGTNACC 1 cut(s) 3434
EcoRI GAATTC 2 cut(s) 1206, 1805
EcoRII CCWGG 6 cut(s) 2406, 2545, 3093, 3348, 3817, 4762
EcoT14I CCWWGG 1 cut(s) 4008
EcoT22I ATGCAT 5 cut(s) 1247, 1875, 1927, 3590, 4183
EcoT38I GRGCYC 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 4008
FalI AAGNNNNNCTT 6 cut(s) 315, 347, 2202, 2234, 4878, 4910
FaqI GGGAC 4 cut(s) 361, 1467, 1778, 2627
FauNDI CATATG 1 cut(s) 266
FbaI TGATCA 2 cut(s) 3777, 4933
FblI GTMKAC 1 cut(s) 3276
FokI GGATG 6 cut(s) 522, 772, 1201, 1555, 1645, 3687
FriOI GRGCYC 1 cut(s) 91
GlaI GCGC 3 cut(s) 282, 294, 2143
GsaI CCCAGC 2 cut(s) 72, 4586
GsuI CTGGAG 4 cut(s) 597, 1431, 1734, 4127
HaeII RGCGCY 2 cut(s) 296, 2145
HapII CCGG 3 cut(s) 98, 124, 1802
HgaI GACGC 1 cut(s) 439
HhaI GCGC 3 cut(s) 283, 295, 2144
Hin1I GRCGYC 1 cut(s) 431
Hin6I GCGC 3 cut(s) 281, 293, 2142
HinP1I GCGC 3 cut(s) 281, 293, 2142
HincII GTYRAC 1 cut(s) 3502
HindII GTYRAC 1 cut(s) 3502
HindIII AAGCTT 1 cut(s) 1769
HpaII CCGG 3 cut(s) 98, 124, 1802
HpyCH4IV ACGT 7 cut(s) 448, 753, 1195, 3214, 3417, 4331, 4567
HpySE526I ACGT 7 cut(s) 448, 753, 1195, 3214, 3417, 4331, 4567
Hsp92I GRCGYC 1 cut(s) 431
HspAI GCGC 3 cut(s) 281, 293, 2142
Kpn2I TCCGGA 1 cut(s) 1801
Ksp22I TGATCA 2 cut(s) 3777, 4933
LguI GCTCTTC 1 cut(s) 1620
MaeII ACGT 7 cut(s) 448, 753, 1195, 3214, 3417, 4331, 4567
MbiI CCGCTC 2 cut(s) 45, 3100
MfeI CAATTG 3 cut(s) 134, 3459, 3843
MflI RGATCY 7 cut(s) 106, 1158, 1277, 2125, 2270, 2325, 3362
MhlI GDGCHC 4 cut(s) 91, 1540, 2438, 4894
MlsI TGGCCA 1 cut(s) 3822
MluNI TGGCCA 1 cut(s) 3822
MlyI GAGTC 7 cut(s) 83, 1058, 1353, 2018, 2021, 2114, 4040
MmeI TCCRAC 7 cut(s) 1575, 2100, 3136, 3466, 4152, 4383, 4533
Mox20I TGGCCA 1 cut(s) 3822
Mph1103I ATGCAT 5 cut(s) 1247, 1875, 1927, 3590, 4183
MroI TCCGGA 1 cut(s) 1801
MroXI GAANNNNTTC 2 cut(s) 1210, 2400
MscI TGGCCA 1 cut(s) 3822
MslI CAYNNNNRTG 4 cut(s) 212, 269, 540, 762
Msp20I TGGCCA 1 cut(s) 3822
MspA1I CMGCKG 3 cut(s) 1097, 3507, 4883
MspCI CTTAAG 3 cut(s) 1304, 1966, 4190
MspI CCGG 3 cut(s) 98, 124, 1802
MspR9I CCNGG 7 cut(s) 124, 2408, 2547, 3095, 3350, 3819, 4764
MunI CAATTG 3 cut(s) 134, 3459, 3843
Mva1269I GAATGC 2 cut(s) 3491, 4522
MvaI CCWGG 6 cut(s) 2408, 2547, 3095, 3350, 3819, 4764
MvnI CGCG 1 cut(s) 2685
NciI CCSGG 1 cut(s) 124
NdeI CATATG 1 cut(s) 266
NheI GCTAGC 2 cut(s) 1511, 3250
NmuCI GTSAC 9 cut(s) 866, 1524, 1723, 1970, 2131, 3344, 3402, 3413, 3434
NruI TCGCGA 1 cut(s) 2685
NsiI ATGCAT 5 cut(s) 1247, 1875, 1927, 3590, 4183
NspI RCATGY 6 cut(s) 802, 1929, 3017, 3182, 3410, 4880
NspV TTCGAA 2 cut(s) 1766, 4326
PaeI GCATGC 3 cut(s) 802, 3182, 4880
PagI TCATGA 2 cut(s) 426, 4116
PasI CCCWGGG 1 cut(s) 2407
PceI AGGCCT 1 cut(s) 4835
PciSI GCTCTTC 1 cut(s) 1620
PctI GAATGC 2 cut(s) 3491, 4522
PdmI GAANNNNTTC 2 cut(s) 1210, 2400
PfeI GAWTC 6 cut(s) 326, 1309, 1420, 2181, 2852, 3371
Pfl23II CGTACG 1 cut(s) 4850
PfoI TCCNGGA 1 cut(s) 2545
PleI GAGTC 7 cut(s) 82, 1058, 1352, 2018, 2020, 2113, 4039
PpsI GAGTC 7 cut(s) 82, 1058, 1352, 2018, 2020, 2113, 4039
Ppu21I YACGTR 1 cut(s) 449
PpuMI RGGWCCY 2 cut(s) 26, 348
PshBI ATTAAT 1 cut(s) 1013
Psp124BI GAGCTC 1 cut(s) 91
Psp1406I AACGTT 3 cut(s) 3214, 4331, 4567
Psp5II RGGWCCY 2 cut(s) 26, 348
Psp6I CCWGG 6 cut(s) 2406, 2545, 3093, 3348, 3817, 4762
PspEI GGTNACC 1 cut(s) 3434
PspFI CCCAGC 2 cut(s) 68, 4582
PspGI CCWGG 6 cut(s) 2406, 2545, 3093, 3348, 3817, 4762
PspLI CGTACG 1 cut(s) 4850
PspPI GGNCC 8 cut(s) 26, 348, 767, 2374, 2543, 3637, 3995, 4760
PspPPI RGGWCCY 2 cut(s) 26, 348
PstNI CAGNNNCTG 2 cut(s) 795, 1505
PsuI RGATCY 7 cut(s) 106, 1158, 1277, 2125, 2270, 2325, 3362
PvuII CAGCTG 1 cut(s) 1097
RruI TCGCGA 1 cut(s) 2685
RsaI GTAC 8 cut(s) 889, 1111, 1888, 3699, 3922, 4485, 4852, 4901
RsaNI GTAC 8 cut(s) 888, 1110, 1887, 3698, 3921, 4484, 4851, 4900
RseI CAYNNNNRTG 4 cut(s) 212, 269, 540, 762
SacI GAGCTC 1 cut(s) 91
SapI GCTCTTC 1 cut(s) 1620
Sau96I GGNCC 8 cut(s) 26, 348, 767, 2374, 2543, 3637, 3995, 4760
SchI GAGTC 7 cut(s) 83, 1058, 1353, 2018, 2021, 2114, 4040
ScrFI CCNGG 7 cut(s) 124, 2408, 2547, 3095, 3350, 3819, 4764
SduI GDGCHC 4 cut(s) 91, 1540, 2438, 4894
SfcI CTRYAG 3 cut(s) 2185, 2698, 3357
SfuI TTCGAA 2 cut(s) 1766, 4326
SinI GGWCC 5 cut(s) 26, 348, 767, 2374, 2543
SmiMI CAYNNNNRTG 4 cut(s) 212, 269, 540, 762
SmlI CTYRAG 8 cut(s) 338, 1180, 1304, 1966, 2360, 3375, 4190, 4341
SmoI CTYRAG 8 cut(s) 338, 1180, 1304, 1966, 2360, 3375, 4190, 4341
SnaBI TACGTA 1 cut(s) 449
SphI GCATGC 3 cut(s) 802, 3182, 4880
SseBI AGGCCT 1 cut(s) 4835
SspI AATATT 4 cut(s) 927, 2535, 3340, 4351
SstI GAGCTC 1 cut(s) 91
StuI AGGCCT 1 cut(s) 4835
StyD4I CCNGG 7 cut(s) 122, 2406, 2545, 3093, 3348, 3817, 4762
StyI CCWWGG 1 cut(s) 4008
TaiI ACGT 7 cut(s) 451, 756, 1198, 3217, 3420, 4334, 4570
TaqII GACCGA 2 cut(s) 1395, 2391
TatI WGTACW 4 cut(s) 887, 1886, 3697, 4483
TauI GCSGC 5 cut(s) 16, 40, 3327, 3521, 3638
TfiI GAWTC 6 cut(s) 326, 1309, 1420, 2181, 2852, 3371
TseFI GTSAC 9 cut(s) 866, 1524, 1723, 1970, 2131, 3344, 3402, 3413, 3434
Tsp45I GTSAC 9 cut(s) 866, 1524, 1723, 1970, 2131, 3344, 3402, 3413, 3434
TspGWI ACGGA 2 cut(s) 1555, 1711
Vha464I CTTAAG 3 cut(s) 1304, 1966, 4190
VneI GTGCAC 1 cut(s) 1536
VpaK11BI GGWCC 5 cut(s) 26, 348, 767, 2374, 2543
VspI ATTAAT 1 cut(s) 1013
XagI CCTNNNNNAGG 1 cut(s) 3929
XbaI TCTAGA 2 cut(s) 809, 3067
XceI RCATGY 6 cut(s) 802, 1929, 3017, 3182, 3410, 4880
XmiI GTMKAC 1 cut(s) 3276
XmnI GAANNNNTTC 2 cut(s) 1210, 2400
Zsp2I ATGCAT 5 cut(s) 1247, 1875, 1927, 3590, 4183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.