FvH4_1g01480

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004795
Physical Location & Seq
Forward (+)
774738 .. 787374
12637 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g01480.t10

Sequence Viewer

Length: 4911 bp
ATGGGCGACGCGCCTCCCCCAGCGGAGAGTGGGGATGTGGAGTCGGGTCATGGAGCTCCGAGGCCGGAGAAGGATCCGAGGAGGGTTGCTCGGAAGTATCAATTGGAGCTGTGTCAGAAAGCTTTGGAGGAGAATATTATTGTCTACCTGGAGACTGGCTGTGGAAAAACTCACATAGCGGTGCTGCTCATGTATGAGCTGCGCCACTTGATACTCAAACCTCAGAAGAACATATGTGTGTTTCTTGCGCCTACGGTGGCGCTTGTTCAGCAGCAAGCAAGGGTTATAGAAGATTCCCTTGATTTGAAGGTTGGGACCTATTGTGGAAGCTCCAGGCAATTGAAAACTCATCAAGACTGGGAAAAAGAGATTGAACAATATGAGGTTCTTGTTATGACTCCTCAAATACTGCTGCGTAACTTGTACCACCGTATTATTAAGATGGAGATAATTGCATTGCTGATTTTTGATGAGTGTCATCATGCGCAAATCACAAGCAACCATCCTTATGCAGAAATTATGCGATTCTGCAAGAGTGATGTCACAAAACTTCCTCGTATATTTGGAATGACTGCATCTCCAGTTGTAGGCGCTTCTAGTCAAGCGAACTTATCAAAGAGTATCAATAGTCTTGAAAATTTACTTGATGCTAAGGTTTATTCGGTTGAAGACAAGACAGAATTGAATTGTTCTGTTGTTTCTCCTGTTATACATGTACATAGTTATAGTCCTGGTATTAGTGGCACATCCAGCCCCTTCATGACTCTTGATAGTAAACTTGAGCAGGTGAAACGCCAGTGCGTAGCGGAACTTGGTAAGAAGACGAGTGACTACCAACGTCTAAGGAGTACAAAGAAAACATTAAAGAGAGTGCATGACAGCATAATGTTTTGCTTGGAAAATCTTGGCCTCTGGGGTGCATTACAAGCTAAACATATTGTTTCGAGTGGCGATCACTTTGAACGAAATGAATTAATAGAGGAAGGCAATAATGGTATAGAGGCAGAGCAAAATAATAGTGATGATACTGTGTGTGCTGAATATCTGGCTCAGGCAGCTGACATCATTCGCACCGACTATGTGAAAGATGCTGTAGCATCTGGTCTATCTTGTATAGATATCTTAAAGGAGCCGTTTTTCTCAAGTAAGGTCTTACGTCTTATTGGAATTCTTTCATCCTCCAGGTTGCAACAGAATATGAAATGCATAATATTTGTCAATAGGATTGTTACTGCAAGATCCTTGTCCTACATACTACAAAACCTTAAGAATCTAGCATCTTGGAAGTGCGATTTTCTTGTAGGAGTCCACTCTAAACTGAAGAGTATGTCAAGGAAAACAATGCAGATCACTCTTGATAAGTTTCGGTCTGGAGAGTTGAATCTATTGGTTGCAACTAAAGTTGGTGAAGAGGGTCTAGATATTCAGACTTGCAGCCTTGTGATACGATTTGATCTTCCAGAAACTGTTGCTAGCTTTATACAGTCACGAGGTCGTGCACGGATGCCTCAGTCTGAATATGTATTTTTGGTAAACAGTGGTAGTCAGAAAGAGTTAGATTTAATAGAAAACTTCAGAAAAGATGAAGATCGAATGAATATGGAGATTTCTTTCAGAACATCCAGTGAGACTTTTATTAGTCCGGAGGAACAAACCTATAAAGTTGCTTCTTCTGGAGCCTCCATCACTTCTGGATATAGCATCTCCTTGTTACACCAGTATTGTTCGAAGCTTCCACATGATGAGTATTATGTCCCCAGTCCAGAATTCTATTTTCTTGGTGATTTGGAGGGAACTATTTGCCATATAATTTTACCTTCAAATGCTCCAATGCATCAAATTGTCAGTGCACCACAATTTTCAATGGAAGATGCCAAAAGAGATGCTTGTCTAAAGGCCATTGAAGAATTGCATAAATTGGGTGCCTTAAGTGACTATCTCTTGCCACTGCAAGACAATGCAAATGTGGAGGAGCTTTTGCAAGACTCTTCTGATTCTGACAGTTTAGAAGATGAAGACTCACGAGGGGAACTACATGAAATGCTGGTTCCTGCTGTCCTAAAAGAGTCATGGAATAAGTCGGAGGATCTTGTCACTCTCAGCTCTTACTATATACAATTTGATCCTTATCCTAATGATAGAATCTATAAAAGTTTTGGTCTATTCGTCAAGGCACCTCTTCCAGCGGAGGCTGAATCAATGGAACTTGATCTTCATCTCGCTCATGGTAGATCTGTGATGACAAAGTTGGTGCCATCTGGATTTGCAGAATTTGTCAAAGATGAGATCTTGCTGGCTCAAAACTTTCAAGAAATGTTCCTCAAGTTCATTCTGGACCGATCAGAATTTGTTTCAGAATTTGTTCCACTGGGAAAGTACGAATTTAGTGGATCGAGCTCATCAACCTTCTACCTATTGCTTCCGGTAACTTTGGGTGAAAATGATAAGATAAGCATAGATTGGAGGATTATCAAAAAATGTTTATCCTCTCCAGTATTCAGGGGCCCGGGACACGCTATGGACAGTAAAATTACATCTTCGGGTATTCGACTTGCATCTGGTTATACAAGCATTAGTGAAGTTGAGGATAGTATAGTTTACGTCTCATATAAGAAGTCATTTTACTTCATCACTAATGTTTCTCGAGAAAGAAATGCCTACAGCCTATATAAAGAAGATCCGGAACCTTTAATCTATGTGGATCATCTTAGTAAGAAGTTTGACATTTCTCTCATATACCCTGAGCAACCTCTTCTGTGTGCAAAACCAGTGTTCTCTTTGCACAACTTGCTACACAATCGTAGGCAAGAAGATTCAGAAGCACAACAACTAGACGAGTATTTTATATATCTGCCTCCTGAGCTTTGTGAGCTGAAGGTAATTGGTTTTTCAAAAGATATCGGAAGTTCAGTGTCTCTGTTACCATCAATCATGCACCGTCTGGAAAACTTGCTTGTCGCCATCGAACTAAAGCATGTATTATGTACTTCATTCCCTGAAGGAGCCGAGGTTACTGCCCAGAGAGTTCTAGAAGCACTCACAACTGAGAAGTGCCAGGAGCGATTTTCCCTTGAAAGGCTTGAACTACTAGGTGATGCTTTCCTCAAATTTGCAGTCGGACGGCACTTTTTCCTTTTGCATGCATTGCTTGATGAAGGTCAACTAACAAGGAAGCGTTCAAATGTGGTAAACAATTCCAATTTACTGAAGCTAGCTACTAGAAGCAACTTACAGGTGTACATACGGGATCAGCCATTTGAACCCTCTCAGTTCTTTGCTTTAGGCCGTCCTTGCAAAAATATTTGTGACCAGGAGACAATAGGATCTATTGATTCTCAAGATTTATGCAGTGCAGTAAAACACTCACATGATTGTGAAGTCAGATGTAGTAAGGGTCACCATTGGTTACATAAGAAAACAATTGCTGATGTTGTTGAAGCCCTTGTTGGAGCATTCATAGTTGACAGCGGTTTTAAAGCTGCTACTGCATTTCTCAGATGGATTGGCATAAAAGTAGAATTCGAAGCATCAGAAGTTACTCAAGTATGCATAGCAAGCAGCAGATACATTCCACTTGCTGCTCGCATAGACATTGCTGCCCTTGAAACTTCATTAGGGTATAAGTTTCTCCACAGAGGGTTGCTGTTACAGGCATTTGTACATCCTTCTTACAACAAAAATGGAGGAGGTTGCTATCAGAGATTGGAGTTTCTTGGAGATGCTGTCTTGGATTACTTGATCACCTCATACCTGTATTCAGTTTATCCAAAGTTAAAGCCTGGCCATATGACAGACTTGAGATCAATTTCTGTGAATAATAAAGCATTTGCTACTGTTGCAGTAGCTCGATCCTTCCACAAGTTTCTAGTCTCTGACTCCTGTACCCTTTCTAAGGCTATAAAGACTTATGTGAACTTTGTTGAGACATCTGCATCAGATAGTAGTCTGGTTGATGGGCCAACATGCCCCAAGGCTCTGGGCGACTTGGTAGAGTCTTGTCTTGGTGCAATTCTTCTTGATACAGGATTCGACTTGAATCGTGTTTGGAATATAATGCTATCGTTTCTGAAACCAGTCATGAGCTTCTCCAACGTTCAGCTTAGTCCTGTTAGGGAACTACGGGAACTTTGCCAAGCTCATGCATGGGATCTTAAATTTCTTCCATCAAAGAAGGGTAAAACATTTTCAATTGAGGCAACAGTGAAGGGGAATAATGTTTCTGCTACTGCTTCCTCAACTGGCTTGAACAAAAAGGATACTATTAAAATTTCTGCTCAATTAATTTTCGAAAAGTTGAAGGCTCAAGGCAATATACCGAAGTCTAAGTTAACTTTGGAGGAAGTCTTAAAGTCATGCTGCAAGATGGAAGCCAAACTTATCGGATATGATGAAACACCAATAGATGTTACAGCTCCTGATATAATTGGATTTGAGAACTTAAAGGTACAAGAGCCTTCTAGCAGCAGTTCCAACTCAGATGTGCATTCTATCAGTGAAGCAAGCAGTAGTCACTCCTGCGTAAAGCGTGTAGGACAATCCCCAGCATCAAGTGGAGCAGTCAAAATGGACAGCCATGATTCCTGTAACAATCATAGCTCTGATGCTGACTCAAAAACCAGAGGTGTGCCACACAATTTAACAGCAAGATCGCATCTGTATGAAGCCTGTGCTGCAAACTATTGGGAACCCCCTGTATTTGAATGTTGCCAAGAGGAAGGACCAAGCCACTTAAAATCGTTCATTTACAAGGTTACAGTAAAGATAGATGATGCTTCAGATATGCTGTTGGAGGCCAATAGCGCACCTCGTACGAGCAAGAAAGCAGCAGCAGAGCATGCAGCGGAAGGCGCACTTTGGTACTTGGAAAAGAAAGGGTACATAGAGAAGAGAAGTCAAACAGAGGAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006351 GO:0006353 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009798 GO:0009888 GO:0009892 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097659 GO:0098542 GO:0098586 GO:0098727 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1637

Amino Acids

182.91

Weight (kDa)

5.98

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 133 - 247 1.6e-18 Helicase conserved C-terminal domain
Dicer_dimer PF03368 314 - 399 3.1e-23 Dicer dimerisation domain
PAZ PF02170 607 - 727 6.5e-09 PAZ domain
Ribonucleas_3_3 PF14622 771 - 829 1.5e-06 Ribonuclease-III-like
Ribonuclease_3 PF00636 772 - 904 1.7e-17 Ribonuclease III domain
Ribonucleas_3_3 PF14622 960 - 1091 1.1e-13 Ribonuclease-III-like
Ribonuclease_3 PF00636 980 - 1089 7.8e-21 Ribonuclease III domain
DND1_DSRM PF14709 1294 - 1369 1.2e-14 double strand RNA binding domain from DEAD END PROTEIN 1
dsrm PF00035 1296 - 1365 4.9e-06 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 775
Acc16I TGCGCA 1 cut(s) 486
Acc36I ACCTGC 1 cut(s) 775
AccB1I GGYRCC 3 cut(s) 1922, 2173, 2251
AccI GTMKAC 1 cut(s) 144
AccII CGCG 1 cut(s) 11
AccIII TCCGGA 2 cut(s) 1642, 2680
AciI CCGC 6 cut(s) 23, 179, 806, 2186, 3468, 4841
AclI AACGTT 1 cut(s) 4092
AcoI YGGCCR 1 cut(s) 3781
AcuI CTGAAG 6 cut(s) 1340, 1558, 2894, 3018, 3227, 4758
AfiI CCNNNNNNNGG 3 cut(s) 587, 3075, 3892
AflII CTTAAG 2 cut(s) 1265, 1927
AflIII ACRYGT 1 cut(s) 712
AjnI CCWGG 7 cut(s) 147, 332, 730, 1181, 3054, 3309, 3778
AjuI GAANNNNNNNTTGG 4 cut(s) 86, 118, 3429, 3461
Alw21I GWGCWC 4 cut(s) 58, 1501, 1852, 2399
Alw26I GTCTC 7 cut(s) 146, 1622, 2608, 2919, 3308, 3874, 3917
Alw44I GTGCAC 2 cut(s) 1497, 1848
AlwNI CAGNNNCTG 2 cut(s) 1466, 4415
Ama87I CYCGRG 2 cut(s) 2506, 2643
Aor13HI TCCGGA 2 cut(s) 1642, 2680
AoxI GGCC 8 cut(s) 62, 907, 1896, 2503, 3283, 3781, 3956, 4791
ApaI GGGCCC 1 cut(s) 2507
ApaLI GTGCAC 2 cut(s) 1497, 1848
ArsI GACNNNNNNTTYG 2 cut(s) 3099, 3131
AseI ATTAAT 2 cut(s) 974, 4280
Asp700I GAANNNNTTC 2 cut(s) 1171, 2361
AspLEI GCGC 8 cut(s) 13, 204, 250, 262, 487, 593, 4802, 4850
AspS9I GGNCC 6 cut(s) 315, 2335, 2503, 2504, 3956, 4718
AsuC2I CCSGG 2 cut(s) 2507, 2508
AsuHPI GGTGA 7 cut(s) 799, 1418, 1793, 2447, 3104, 3389, 3733
AsuII TTCGAA 3 cut(s) 1727, 3522, 4287
AsuNHI GCTAGC 2 cut(s) 1472, 3211
AvaI CYCGRG 2 cut(s) 2506, 2643
AvaII GGWCC 3 cut(s) 315, 2335, 4718
BaeGI GKGCMC 3 cut(s) 1501, 1852, 2507
BaeI ACNNNNGTAYC 2 cut(s) 3865, 3898
BalI TGGCCA 1 cut(s) 3783
BamHI GGATCC 1 cut(s) 73
BanI GGYRCC 3 cut(s) 1922, 2173, 2251
BanII GRGCYC 3 cut(s) 58, 2399, 2507
BarI GAAGNNNNNNTAC 2 cut(s) 2606, 2638
BauI CACGAG 2 cut(s) 1488, 2022
BbsI GAAGAC 3 cut(s) 675, 827, 2022
Bbv12I GWGCWC 4 cut(s) 58, 1501, 1852, 2399
BceAI ACGGC 3 cut(s) 1117, 3137, 3270
BcgI CGANNNNNNTGC 2 cut(s) 2996, 3030
BciT130I CCWGG 7 cut(s) 149, 334, 732, 1183, 3056, 3311, 3780
BciVI GTATCC 1 cut(s) 4249
BclI TGATCA 1 cut(s) 3738
BcnI CCSGG 2 cut(s) 2507, 2508
BcoDI GTCTC 7 cut(s) 146, 1622, 2608, 2919, 3308, 3874, 3917
BfmI CTRYAG 2 cut(s) 1092, 2659
BfoI RGCGCY 2 cut(s) 263, 594
BfrI CTTAAG 2 cut(s) 1265, 1927
BfuAI ACCTGC 1 cut(s) 775
BfuI GTATCC 1 cut(s) 4249
BglII AGATCT 2 cut(s) 2231, 2286
Bme1390I CCNGG 9 cut(s) 149, 334, 732, 1183, 2507, 2508, 3056, 3311, 3780
Bme18I GGWCC 3 cut(s) 315, 2335, 4718
BmeT110I CYCGRG 2 cut(s) 2506, 2643
BmgT120I GGNCC 6 cut(s) 315, 2335, 2503, 2504, 3956, 4718
BmrFI CCNGG 9 cut(s) 149, 334, 732, 1183, 2507, 2508, 3056, 3311, 3780
BmrI ACTGGG 3 cut(s) 367, 1752, 2378
BmtI GCTAGC 2 cut(s) 1476, 3215
BmuI ACTGGG 3 cut(s) 367, 1752, 2378
BpiI GAAGAC 3 cut(s) 675, 827, 2022
BplI GAGNNNNNCTC 2 cut(s) 73, 105
BpmI CTGGAG 7 cut(s) 170, 316, 564, 1165, 1392, 1695, 2475
Bpu10I CCTNAGC 4 cut(s) 651, 1050, 2742, 2859
Bpu14I TTCGAA 3 cut(s) 1727, 3522, 4287
BpuEI CTTGAG 7 cut(s) 800, 1126, 2306, 3321, 3525, 3817, 4287
BpuMI CCSGG 2 cut(s) 2507, 2508
BsaBI GATNNNNATC 3 cut(s) 1587, 2127, 2214
BsaJI CCNNGG 5 cut(s) 59, 77, 2506, 3006, 3969
BsaWI WCCGGW 3 cut(s) 1642, 2422, 2680
BsaXI ACNNNNNCTCC 6 cut(s) 562, 592, 2994, 3024, 3708, 3738
Bsc4I CCNNNNNNNGG 3 cut(s) 587, 3075, 3892
Bse3DI GCAATG 3 cut(s) 455, 3143, 3591
Bse8I GATNNNNATC 3 cut(s) 1587, 2127, 2214
BseAI TCCGGA 2 cut(s) 1642, 2680
BseBI CCWGG 7 cut(s) 149, 334, 732, 1183, 3056, 3311, 3780
BseDI CCNNGG 5 cut(s) 59, 77, 2506, 3006, 3969
BseGI GGATG 7 cut(s) 40, 502, 746, 1175, 1509, 1619, 3661
BseJI GATNNNNATC 3 cut(s) 1587, 2127, 2214
BseLI CCNNNNNNNGG 3 cut(s) 587, 3075, 3892
BseMI GCAATG 3 cut(s) 455, 3143, 3591
BseRI GAGGAG 5 cut(s) 94, 143, 390, 1985, 3699
BseSI GKGCMC 3 cut(s) 1501, 1852, 2507
BseYI CCCAGC 2 cut(s) 19, 4540
BsgI GTGCAG 1 cut(s) 3372
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 8 cut(s) 64, 909, 1898, 2505, 3285, 3783, 3958, 4793
BshNI GGYRCC 3 cut(s) 1922, 2173, 2251
BsiHKAI GWGCWC 4 cut(s) 58, 1501, 1852, 2399
BsiHKCI CYCGRG 2 cut(s) 2506, 2643
BsiSI CCGG 5 cut(s) 65, 1643, 2423, 2507, 2681
BsiWI CGTACG 1 cut(s) 4808
BslFI GGGAC 3 cut(s) 328, 1739, 2523
BslI CCNNNNNNNGG 3 cut(s) 587, 3075, 3892
BsmAI GTCTC 7 cut(s) 146, 1622, 2608, 2919, 3308, 3874, 3917
BsmBI CGTCTC 1 cut(s) 2608
BsmFI GGGAC 3 cut(s) 328, 1739, 2523
BsmI GAATGC 2 cut(s) 3452, 4483
BsnI GGCC 8 cut(s) 64, 909, 1898, 2505, 3285, 3783, 3958, 4793
BsoBI CYCGRG 2 cut(s) 2506, 2643
Bsp119I TTCGAA 3 cut(s) 1727, 3522, 4287
Bsp120I GGGCCC 1 cut(s) 2503
Bsp1286I GDGCHC 5 cut(s) 58, 1501, 1852, 2399, 2507
Bsp13I TCCGGA 2 cut(s) 1642, 2680
Bsp1407I TGTACA 3 cut(s) 715, 3237, 3658
BspACI CCGC 6 cut(s) 23, 179, 806, 2186, 3468, 4841
BspANI GGCC 8 cut(s) 64, 909, 1898, 2505, 3285, 3783, 3958, 4793
BspEI TCCGGA 2 cut(s) 1642, 2680
BspFNI CGCG 1 cut(s) 11
BspHI TCATGA 2 cut(s) 759, 4077
BspMI ACCTGC 1 cut(s) 775
BspOI GCTAGC 2 cut(s) 1476, 3215
BspT104I TTCGAA 3 cut(s) 1727, 3522, 4287
BspT107I GGYRCC 3 cut(s) 1922, 2173, 2251
BspTI CTTAAG 2 cut(s) 1265, 1927
BsrDI GCAATG 3 cut(s) 455, 3143, 3591
BsrGI TGTACA 3 cut(s) 715, 3237, 3658
BssECI CCNNGG 5 cut(s) 59, 77, 2506, 3006, 3969
BssSI CACGAG 2 cut(s) 1488, 2022
BssT1I CCWWGG 1 cut(s) 3969
Bst2BI CACGAG 2 cut(s) 1488, 2022
Bst2UI CCWGG 7 cut(s) 149, 334, 732, 1183, 3056, 3311, 3780
Bst6I CTCTTC 6 cut(s) 1316, 1404, 1993, 2184, 2757, 4880
BstAFI CTTAAG 2 cut(s) 1265, 1927
BstAPI GCANNNNNTGC 3 cut(s) 2788, 3145, 4835
BstAUI TGTACA 3 cut(s) 715, 3237, 3658
BstBI TTCGAA 3 cut(s) 1727, 3522, 4287
BstC8I GCNNGC 9 cut(s) 276, 1474, 2295, 3141, 3213, 3556, 3583, 4501, 4836
BstEII GGTNACC 1 cut(s) 3395
BstENI CCTNNNNNAGG 1 cut(s) 3890
BstF5I GGATG 7 cut(s) 40, 502, 746, 1175, 1509, 1619, 3661
BstFNI CGCG 1 cut(s) 11
BstH2I RGCGCY 2 cut(s) 263, 594
BstHHI GCGC 8 cut(s) 13, 204, 250, 262, 487, 593, 4802, 4850
BstMAI GTCTC 7 cut(s) 146, 1622, 2608, 2919, 3308, 3874, 3917
BstNI CCWGG 7 cut(s) 149, 334, 732, 1183, 3056, 3311, 3780
BstNSI RCATGY 5 cut(s) 716, 2978, 3143, 3966, 4838
BstPI GGTNACC 1 cut(s) 3395
BstSCI CCNGG 9 cut(s) 147, 332, 730, 1181, 2505, 2506, 3054, 3309, 3778
BstSFI CTRYAG 2 cut(s) 1092, 2659
BstSLI GKGCMC 3 cut(s) 1501, 1852, 2507
BstUI CGCG 1 cut(s) 11
BstV2I GAAGAC 3 cut(s) 675, 827, 2022
BstX2I RGATCY 8 cut(s) 73, 1238, 2086, 2231, 2286, 2677, 3323, 4147
BstXI CCANNNNNNTGG 1 cut(s) 3976
BstYI RGATCY 8 cut(s) 73, 1238, 2086, 2231, 2286, 2677, 3323, 4147
BsuI GTATCC 1 cut(s) 4249
BsuRI GGCC 8 cut(s) 64, 909, 1898, 2505, 3285, 3783, 3958, 4793
BtsCI GGATG 7 cut(s) 40, 502, 746, 1175, 1509, 1619, 3661
BtsI GCAGTG 2 cut(s) 1946, 3355
BveI ACCTGC 1 cut(s) 775
Cac8I GCNNGC 9 cut(s) 276, 1474, 2295, 3141, 3213, 3556, 3583, 4501, 4836
CaiI CAGNNNCTG 2 cut(s) 1466, 4415
CciI TCATGA 2 cut(s) 759, 4077
CfoI GCGC 8 cut(s) 13, 204, 250, 262, 487, 593, 4802, 4850
Cfr13I GGNCC 6 cut(s) 315, 2335, 2503, 2504, 3956, 4718
Cfr9I CCCGGG 1 cut(s) 2506
CseI GACGC 1 cut(s) 17
DraI TTTAAA 1 cut(s) 3475
EaeI YGGCCR 1 cut(s) 3781
Eam1104I CTCTTC 6 cut(s) 1316, 1404, 1993, 2184, 2757, 4880
EarI CTCTTC 6 cut(s) 1316, 1404, 1993, 2184, 2757, 4880
Ecl136II GAGCTC 2 cut(s) 56, 2397
Eco130I CCWWGG 1 cut(s) 3969
Eco24I GRGCYC 3 cut(s) 58, 2399, 2507
Eco32I GATATC 2 cut(s) 1120, 2899
Eco47I GGWCC 3 cut(s) 315, 2335, 4718
Eco53kI GAGCTC 2 cut(s) 56, 2397
Eco57I CTGAAG 6 cut(s) 1340, 1558, 2894, 3018, 3227, 4758
Eco88I CYCGRG 2 cut(s) 2506, 2643
Eco91I GGTNACC 1 cut(s) 3395
EcoICRI GAGCTC 2 cut(s) 56, 2397
EcoNI CCTNNNNNAGG 1 cut(s) 3890
EcoO109I RGGNCCY 2 cut(s) 315, 2503
EcoO65I GGTNACC 1 cut(s) 3395
EcoRI GAATTC 3 cut(s) 1167, 1766, 3518
EcoRII CCWGG 7 cut(s) 147, 332, 730, 1181, 3054, 3309, 3778
EcoRV GATATC 2 cut(s) 1120, 2899
EcoT14I CCWWGG 1 cut(s) 3969
EcoT22I ATGCAT 5 cut(s) 1208, 1836, 3145, 3551, 4144
EcoT38I GRGCYC 3 cut(s) 58, 2399, 2507
ErhI CCWWGG 1 cut(s) 3969
Esp3I CGTCTC 1 cut(s) 2608
FalI AAGNNNNNCTT 8 cut(s) 282, 314, 1870, 1902, 4359, 4391, 4836, 4868
FaqI GGGAC 3 cut(s) 328, 1739, 2523
FauNDI CATATG 2 cut(s) 233, 3786
FbaI TGATCA 1 cut(s) 3738
FblI GTMKAC 1 cut(s) 144
FokI GGATG 7 cut(s) 47, 489, 733, 1162, 1516, 1606, 3648
FriOI GRGCYC 3 cut(s) 58, 2399, 2507
FspI TGCGCA 1 cut(s) 486
GlaI GCGC 8 cut(s) 12, 203, 249, 261, 486, 592, 4801, 4849
GsaI CCCAGC 2 cut(s) 23, 4544
GsuI CTGGAG 7 cut(s) 170, 316, 564, 1165, 1392, 1695, 2475
HaeII RGCGCY 2 cut(s) 263, 594
HaeIII GGCC 8 cut(s) 64, 909, 1898, 2505, 3285, 3783, 3958, 4793
HapII CCGG 5 cut(s) 65, 1643, 2423, 2507, 2681
HgaI GACGC 1 cut(s) 17
HhaI GCGC 8 cut(s) 13, 204, 250, 262, 487, 593, 4802, 4850
Hin6I GCGC 8 cut(s) 11, 202, 248, 260, 485, 591, 4800, 4848
HinP1I GCGC 8 cut(s) 11, 202, 248, 260, 485, 591, 4800, 4848
HincII GTYRAC 3 cut(s) 3161, 3463, 4329
HindII GTYRAC 3 cut(s) 3161, 3463, 4329
HindIII AAGCTT 2 cut(s) 120, 1730
HpaI GTTAAC 1 cut(s) 4329
HpaII CCGG 5 cut(s) 65, 1643, 2423, 2507, 2681
HphI GGTGA 7 cut(s) 799, 1418, 1793, 2447, 3104, 3389, 3733
Hpy99I CGWCG 1 cut(s) 11
HpyCH4IV ACGT 4 cut(s) 838, 1156, 2601, 4092
HpySE526I ACGT 4 cut(s) 838, 1156, 2601, 4092
HspAI GCGC 8 cut(s) 11, 202, 248, 260, 485, 591, 4800, 4848
Kpn2I TCCGGA 2 cut(s) 1642, 2680
Ksp22I TGATCA 1 cut(s) 3738
KspAI GTTAAC 1 cut(s) 4329
MaeII ACGT 4 cut(s) 838, 1156, 2601, 4092
MfeI CAATTG 4 cut(s) 101, 338, 3420, 4188
MflI RGATCY 8 cut(s) 73, 1238, 2086, 2231, 2286, 2677, 3323, 4147
MhlI GDGCHC 5 cut(s) 58, 1501, 1852, 2399, 2507
MlsI TGGCCA 1 cut(s) 3783
MluNI TGGCCA 1 cut(s) 3783
MmeI TCCRAC 6 cut(s) 2061, 3097, 3427, 4113, 4494, 4767
Mox20I TGGCCA 1 cut(s) 3783
Mph1103I ATGCAT 5 cut(s) 1208, 1836, 3145, 3551, 4144
MroI TCCGGA 2 cut(s) 1642, 2680
MroXI GAANNNNTTC 2 cut(s) 1171, 2361
MscI TGGCCA 1 cut(s) 3783
MslI CAYNNNNRTG 6 cut(s) 179, 236, 507, 3366, 3372, 4656
Msp20I TGGCCA 1 cut(s) 3783
MspA1I CMGCKG 5 cut(s) 23, 1058, 2186, 3468, 4841
MspCI CTTAAG 2 cut(s) 1265, 1927
MspI CCGG 5 cut(s) 65, 1643, 2423, 2507, 2681
MspR9I CCNGG 9 cut(s) 149, 334, 732, 1183, 2507, 2508, 3056, 3311, 3780
MunI CAATTG 4 cut(s) 101, 338, 3420, 4188
Mva1269I GAATGC 2 cut(s) 3452, 4483
MvaI CCWGG 7 cut(s) 149, 334, 732, 1183, 3056, 3311, 3780
MvnI CGCG 1 cut(s) 11
NciI CCSGG 2 cut(s) 2507, 2508
NdeI CATATG 2 cut(s) 233, 3786
NheI GCTAGC 2 cut(s) 1472, 3211
NmeAIII GCCGAG 1 cut(s) 3031
NmuCI GTSAC 8 cut(s) 541, 827, 1485, 1931, 2092, 3305, 3395, 4508
NsbI TGCGCA 1 cut(s) 486
NsiI ATGCAT 5 cut(s) 1208, 1836, 3145, 3551, 4144
NspI RCATGY 5 cut(s) 716, 2978, 3143, 3966, 4838
NspV TTCGAA 3 cut(s) 1727, 3522, 4287
PaeI GCATGC 2 cut(s) 3143, 4838
PaeR7I CTCGAG 1 cut(s) 2643
PagI TCATGA 2 cut(s) 759, 4077
PaqCI CACCTGC 1 cut(s) 775
PciI ACATGT 1 cut(s) 712
PctI GAATGC 2 cut(s) 3452, 4483
PdmI GAANNNNTTC 2 cut(s) 1171, 2361
Pfl23II CGTACG 1 cut(s) 4808
PpuMI RGGWCCY 1 cut(s) 315
PscI ACATGT 1 cut(s) 712
PshBI ATTAAT 2 cut(s) 974, 4280
Psp124BI GAGCTC 2 cut(s) 58, 2399
Psp1406I AACGTT 1 cut(s) 4092
Psp5II RGGWCCY 1 cut(s) 315
Psp6I CCWGG 7 cut(s) 147, 332, 730, 1181, 3054, 3309, 3778
PspEI GGTNACC 1 cut(s) 3395
PspFI CCCAGC 2 cut(s) 19, 4540
PspGI CCWGG 7 cut(s) 147, 332, 730, 1181, 3054, 3309, 3778
PspLI CGTACG 1 cut(s) 4808
PspOMI GGGCCC 1 cut(s) 2503
PspPI GGNCC 6 cut(s) 315, 2335, 2503, 2504, 3956, 4718
PspPPI RGGWCCY 1 cut(s) 315
PstNI CAGNNNCTG 2 cut(s) 1466, 4415
PsuI RGATCY 8 cut(s) 73, 1238, 2086, 2231, 2286, 2677, 3323, 4147
PvuII CAGCTG 1 cut(s) 1058
RseI CAYNNNNRTG 6 cut(s) 179, 236, 507, 3366, 3372, 4656
SacI GAGCTC 2 cut(s) 58, 2399
Sau96I GGNCC 6 cut(s) 315, 2335, 2503, 2504, 3956, 4718
ScrFI CCNGG 9 cut(s) 149, 334, 732, 1183, 2507, 2508, 3056, 3311, 3780
SduI GDGCHC 5 cut(s) 58, 1501, 1852, 2399, 2507
SfcI CTRYAG 2 cut(s) 1092, 2659
Sfr274I CTCGAG 1 cut(s) 2643
SfuI TTCGAA 3 cut(s) 1727, 3522, 4287
SinI GGWCC 3 cut(s) 315, 2335, 4718
SlaI CTCGAG 1 cut(s) 2643
SmaI CCCGGG 1 cut(s) 2508
SmiMI CAYNNNNRTG 6 cut(s) 179, 236, 507, 3366, 3372, 4656
SphI GCATGC 2 cut(s) 3143, 4838
SsiI CCGC 6 cut(s) 23, 179, 806, 2186, 3468, 4841
SspI AATATT 3 cut(s) 136, 1212, 3301
SstI GAGCTC 2 cut(s) 58, 2399
StyD4I CCNGG 9 cut(s) 147, 332, 730, 1181, 2505, 2506, 3054, 3309, 3778
StyI CCWWGG 1 cut(s) 3969
TaiI ACGT 4 cut(s) 841, 1159, 2604, 4095
TaqII GACCGA 2 cut(s) 1356, 2352
TatI WGTACW 5 cut(s) 715, 848, 2984, 3237, 3658
TseFI GTSAC 8 cut(s) 541, 827, 1485, 1931, 2092, 3305, 3395, 4508
Tsp45I GTSAC 8 cut(s) 541, 827, 1485, 1931, 2092, 3305, 3395, 4508
TspGWI ACGGA 1 cut(s) 1516
TspMI CCCGGG 1 cut(s) 2506
Vha464I CTTAAG 2 cut(s) 1265, 1927
VneI GTGCAC 2 cut(s) 1497, 1848
VpaK11BI GGWCC 3 cut(s) 315, 2335, 4718
VspI ATTAAT 2 cut(s) 974, 4280
XagI CCTNNNNNAGG 1 cut(s) 3890
XbaI TCTAGA 2 cut(s) 1417, 3028
XceI RCATGY 5 cut(s) 716, 2978, 3143, 3966, 4838
XhoI CTCGAG 1 cut(s) 2643
XmaI CCCGGG 1 cut(s) 2506
XmiI GTMKAC 1 cut(s) 144
XmnI GAANNNNTTC 2 cut(s) 1171, 2361
Zsp2I ATGCAT 5 cut(s) 1208, 1836, 3145, 3551, 4144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.