Rh2DG019200

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004795
Physical Location & Seq
Reverse (-)
1106053 .. 1119566
13514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG019200.1

Sequence Viewer

Length: 4551 bp
ATGGAACAATATGAGGTTTTTGTCATGACGCCTCAAATACTGTTACGTAACTTATACCACCGTATTATTAAGATGGAGTTGATTGCATTGCTGATTTTTGATGAGTGTCATCACGCTCAAATAAAAAGCAACCATCCTTATGCAGAAATTATGAAATTTTACAAGAATGATCCCACGAAACTTCCTCGTATATTTGGCATGACTGCATCTCCAGTTGTAGGTAAAGGCACTTCTAGTCAATCGAATTTATCAAAGAGTATCAATAGTCTTGAAAATTTACTTGATGCTAAGGTTTATACAGTTGAAGACAAGACAGAATTGCATTGTTCTGTTGTTTCTCCTGTTATCAACGTCCATCGTTATGGTCCTGTTATTAGTGGCACATCCAGCCCCTGCATGCCTTTTTCTAGAAAACTTGACCAGCTGAAACGCCAGTGTATAGCGGAACTTAGTAAGAAGACGAGTGACTTCCAAAGTCTAAGGAGTACAAAGAAAACATTAAACAGAGTGCATGACAGTATAATATTTTGCTTGGAAACTCTTGGCCTCTGGGGTGCGTTACAAGCTAGGCATATTCTTTCCAGTGGTGATAACTTTGAACGAAATGAATTAATAGAGGAAGGCAATAATGATATAGAGGCAGAAGGAAATAATAGTGATGACTCTGTATGTGCGGAATATCTGGCTCAGGCAGCTGACATCATTCGTACTGACTATGTGAAAGATGCTGTTGCATCTGGTCTATCTCGTGTAGAGATCTTAAAGGAGCCATTCTTCTCAAGTAAGGTCTTACGTCTTATTGGAATTCTTTCATCCTTCAGGTTGCAGCAGGATATGAAATGCATAATTTTTGTCAATAGGATTATTACTGCAAGATCCTTGTCCTACATACTACAAAACCTTAAGAATCTAGCATCTTGGAAGTGCGATTTTCTTGTAGGAGTCCACTCGAAACTGAAGAGTATGTCAAGGAAGACGATGCAGATCATTCTTGATAAGTTTCGGTCTGGAGAGTTGAATCTATTGGTTGCAACTAAAGTTGGTGAAGAGGGACTAGATATTCAGACTTGCTGCCTCGTGATACGATTTGATCTTCCAGAAACTGTTGCTAGCTTTATACAGTCACGAGGTCGTGCACGGATGCCTCAGTCTGAATATATATTTTTGGTAAACAGTGGTAGTCAGAAAGAGTTGGATTTAATAGAAAACTTCAGGAAAGATGAAGAGCGAATGAACATGGAAGTTGCTTTCAGAACATCCAGTGAGACTTTTCTTAGTCCTGAGGAACGAACATATAAAGTTGCTTCTTCTGGAGCTTCCGTCACTTCTGGATATAGCATCTCATTGTTACACCTGTATTGTTCGAAGCTTCCACATGATGAGTATTATGTCCCCACTCCGGAATTCTATTTTCTTGGTGATTTGGAGGGAACTATTTGCCATATAATTTTACCTTCCAATGCTCCAATGCATCAAATTGTCAGTACACCACAATCTTCGATGGAAGATGCCAAAAAAGATGCATGTCTAAAAGCCATCGAAGAATTGCATAAATTGGGTGCCTTAAGTGACTATCTCTTGCCACAGCAAGACAATGCAAATGTGGAGGAGTCCATGCTAGACTCTTCTGATTATGACAGCATGGAAGATGAAGACTTACGAGAGGAACTACATGAAATGCTGGTTCCTGCTGTCCTAAAAGAGTCATGGAATAAGTTGGAGGATCTCGTCACCCTCAGCGCTTACTATTTTCAATTTGATCCTGAACCTAATGATAGAATCTATAGAAGTTTTGGTCTATTTGTCAAAGCACCTCTTCCAGCAGAGGCCGAAACAATGGAACTTGATCTTCATCTCGCTCATGGTAGATCTGTGATGACAAAGTTGGTGCCATCAGGATTTGCAGAATTTGTCAAAGATGAGATCTTGCTGGCTCAAAACTTTCAAGAAATGTTCCTCAAGCTCATTCTGGACCGATCAGAATTTGTTTCTGAATTTGTTCCCCTGGGAAACTATGATTTTAGCAGATCGAGCACATCAACCTTCTACCTATTGCTTCCAGTAACTTTGGGTGAAAATGATAAGATAAACATAGATTGGAGGATTATCAAAAAATGTTTATCATCTCCAATATTCAGGGGTCCAGGAGATGCTATGGACAGTAAAATTATATCTTCGGGTATTCGACTTGCATCTGGTTATAGAAGCATCAGTGAAGTTGAGGATAGTTTAGTTTATGTCCCATATAAGAATACATTTTACTTCATCACTAATGTTACTCGCGAAAGAAATGCCTACAGCCAATATAAAGAAAAGTCAGATGCTTTAACCTATGTGGATCATCTAAGTAAAAAGTTTGACATTTCTCTTAAATACCCTGAGCAACATCTTTTGTGTGCAAAACCAGTTTTCAATTTGCACAACTTGCTCCACAATCGTAGGCAAGAAGATTCAGAAGCACAGCAACTAGATGAGTATTATATATATGTGCCTCCTGAGCTTTGCGAGCTGAAGGTAATTGGCTTTTCAAAAGATATTGGGAGTTCGGTATCTCTGTTACCATCAATCATGCACCGTCTGGAAAACTTGCTTGTCGCCATTGAACTGAAGCATGTATTATGCACTTCATTCCCCGAAGGAGCTGAGGTTACTTCGCCGAGAGTTCTAGAAGCCCTCACAACTGAGAAGTGCCAGGAGCGGATTTCCCTTGAAAGGCTTGAAATACTAGGAGATGCTTTCCTCAAATTTGCAGTTGGAAGGCACTTTTTCCTTTTGCATGCTTCACTTGATGAAGGTGAACTAACAAGGAAACGTTCAAATGTGGTAAACAATTCCAATTTATTGAAGCTAGCTACTAGAAGCAACTTACAGGTATACATACGGGATCAGCCATTTGAACCCTCTCAGTTCTTCACTTTAGGCCGCCCTTGCAAAAATATTTGTGACCAGGAGACTATAGGATCTCTTGATTCTCAAGATTTAGGCAGTGCAGTAAAACAGTCACATGCTCGTCACGTCAGATGTAGTAAGGGTCACCATTGGTTACATAAGAAAACAATTGCTGATGTTGTTGAAGCCCTTGTTGGAGCATTCATAGTTGACAGCGGTTTTAAAGCGGCAACTGCATTTCTCAGATGGATTGGCATAAAAGTAAACTTTGAAGCATCAGAAGTTACTAAAGTATGCATAGCAAGCAGCAGATACATTTCACTTGCTGCTTGCATAGACATTGCGGCCCTTGAAAATTCATTAGGGTATCAGTTTCTCCACAGAGGTTTGCTGTTACAGGCATTTGTACATCCTTCTTACAACAAACATGGAGGAGGTTGCTATCAGAGATTGGAGTTTCTTGGAGATGCTGTCTTGGATTACTTGATCACCTCATACCTGTATTCAGTGTATCCAAAGTTAAAGCCTGGCCATTTGACAGATTTGAGATCAATTGCTGTGAATAATAAAGCCTTTGCTACTGTTGCAGTAGCTCGATCCTTCCACAAGTTTCTACTCTCTGACACCAGTACCCTTTCTGAGGCTATAAAGACTTATGTGAACTTCATTGAGACATCTGCATCAGATAGCAGTCTGGTTGATGGGCCAAAATGCCCCAAGGCTCTGGGCGACTTGGTAGAGTCTTGTCTTGGTGCAATTCTTCTTGATACAGGGTTCGACTTAAACCTTGTTTGGAAGATAATGCTATCCTTTCTGGAGCCTGTCATGAGCTTTTCCAACATTCAGCTTAGTCCTGTTAGGGAACTACGGGAACTTTGCCAAGCTCATGCATGGGTTCTTAAGTTTTTACCATCAAAGAAGGGTAAAACATTTTCAGTTGAGGCAACAGTGAATGGGAATAATGTTTCTGCAACTGCTTCCTCAACTGCCCTGAACAAAAGGGAGGCTATTAGAATTTCGGCTCAGTTAATTTTCGAAACGTTGAAGGCTCAAGGCAATATACCAAAGTCTAAGTTGTCTTTGGAGGAAGTCTTGAAGTCAAGCTGCAAGTTGGAACCCAAACTTATCGGATATGATGAAACACCAATAGATGTTACAGCTCTTGATGTTATTGGATTTGATAAGTTGAATGTACAAGAGGCTTCTAGCAGCAGTTCCAACTCAGATGTGCATTCTATGAGTGAAGTCAGCAGTGGTAACTCCTCCTGCATAAAACGTTTACGACAACCCCCAGCTTCACTTGGAGAAGTCAAAATGGACAGCACTAATGCCTGTAACAGTTGTAGCTCTGATGCTATCTCACAAACCAGAGGTGTGCCACACAAATTAACAGCAAAATCTCGTTTGCATGAGACCTGTGTTGCTAACTTCTGGGAACCCCCTGAATTTGAATGTTGCAAAGAGGAAGGGCCAGGCCACATAAAATCGTTCACTTTCAAGGTTACAGTGAAGATAGATGATGTTTCGGATACACTTTTGGAGGCCTTTGGAGAACCTCGTACGAGCAAGAAAGCAGCAGCAGAGCATGCAGCGGAAGGGGCACTTTGGTACTTGGAAAAGAAAGGTTACATAGAGGAGAGTGATCAATCAGAGGAACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006351 GO:0006353 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009798 GO:0009888 GO:0009892 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097659 GO:0098542 GO:0098586 GO:0098727 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1516

Amino Acids

170.03

Weight (kDa)

5.85

Isoelectric Point (pI)

50.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 266 - 379 8.1e-18 Helicase conserved C-terminal domain
Dicer_dimer PF03368 446 - 531 3e-23 Dicer dimerisation domain
PAZ PF02170 739 - 859 1.1e-09 PAZ domain
Ribonucleas_3_3 PF14622 903 - 961 5.4e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 904 - 1036 3e-18 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1092 - 1223 7.1e-14 Ribonuclease-III-like
Ribonuclease_3 PF00636 1112 - 1221 3.3e-21 Ribonuclease III domain
DND1_DSRM PF14709 1427 - 1502 4.5e-14 double strand RNA binding domain from DEAD END PROTEIN 1
dsrm PF00035 1428 - 1498 1.3e-06 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1559, 1888
AccBSI CCGCTC 1 cut(s) 2698
AccI GTMKAC 1 cut(s) 2874
AccII CGCG 1 cut(s) 2283
AccIII TCCGGA 1 cut(s) 1399
AciI CCGC 8 cut(s) 443, 674, 2698, 2923, 3105, 3116, 3233, 4481
AclI AACGTT 3 cut(s) 2812, 3929, 4165
AclWI GGATC 8 cut(s) 164, 870, 1731, 1754, 2346, 2892, 2968, 3480
AcoI YGGCCR 1 cut(s) 3418
AcuI CTGAAG 5 cut(s) 802, 977, 1195, 2531, 2627
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 8 cut(s) 487, 709, 1486, 3297, 3520, 4083, 4450, 4499
AfeI AGCGCT 1 cut(s) 1741
AfiI CCNNNNNNNGG 4 cut(s) 218, 1399, 2712, 3529
AflII CTTAAG 3 cut(s) 902, 1564, 3788
AjiI CACGTC 1 cut(s) 3016
AjnI CCWGG 6 cut(s) 2004, 2143, 2691, 2946, 3415, 4360
AjuI GAANNNNNNNTTGG 2 cut(s) 3066, 3098
Alw21I GWGCWC 2 cut(s) 1138, 2036
Alw26I GTCTC 4 cut(s) 1259, 2945, 3554, 4295
Alw44I GTGCAC 1 cut(s) 1134
AlwI GGATC 8 cut(s) 164, 870, 1731, 1754, 2346, 2892, 2968, 3480
AlwNI CAGNNNCTG 2 cut(s) 393, 1103
Aor13HI TCCGGA 1 cut(s) 1399
Aor51HI AGCGCT 1 cut(s) 1741
AoxI GGCC 9 cut(s) 544, 1827, 2920, 3234, 3418, 3593, 4358, 4363, 4431
ApaLI GTGCAC 1 cut(s) 1134
AseI ATTAAT 1 cut(s) 611
Asp700I GAANNNNTTC 2 cut(s) 808, 1998
AspLEI GCGC 1 cut(s) 1742
AspS9I GGNCC 6 cut(s) 365, 1972, 2141, 3235, 3593, 4358
AsuHPI GGTGA 8 cut(s) 599, 1055, 1430, 1723, 2084, 2807, 3026, 3370
AsuII TTCGAA 2 cut(s) 1364, 3924
AsuNHI GCTAGC 2 cut(s) 1109, 2848
AvaII GGWCC 3 cut(s) 365, 1972, 2141
AxyI CCTNAGG 1 cut(s) 1281
BaeGI GKGCMC 2 cut(s) 1138, 4492
BaeI ACNNNNGTAYC 2 cut(s) 3502, 3535
BalI TGGCCA 1 cut(s) 3420
BanI GGYRCC 2 cut(s) 1559, 1888
BarI GAAGNNNNNNTAC 2 cut(s) 214, 246
BauI CACGAG 3 cut(s) 747, 1076, 1125
BbsI GAAGAC 4 cut(s) 312, 464, 980, 1659
Bbv12I GWGCWC 2 cut(s) 1138, 2036
BbvCI CCTCAGC 2 cut(s) 1736, 2643
BcgI CGANNNNNNTGC 2 cut(s) 2273, 2307
BciT130I CCWGG 6 cut(s) 2006, 2145, 2693, 2948, 3417, 4362
BciVI GTATCC 2 cut(s) 3411, 4411
BclI TGATCA 2 cut(s) 3375, 4531
BcoDI GTCTC 4 cut(s) 1259, 2945, 3554, 4295
BfmI CTRYAG 3 cut(s) 1783, 2296, 2955
BfoI RGCGCY 1 cut(s) 1743
BfrI CTTAAG 3 cut(s) 902, 1564, 3788
BfuI GTATCC 2 cut(s) 3411, 4411
BglII AGATCT 3 cut(s) 756, 1868, 1923
Bme1390I CCNGG 6 cut(s) 2006, 2145, 2693, 2948, 3417, 4362
Bme18I GGWCC 3 cut(s) 365, 1972, 2141
BmgBI CACGTC 1 cut(s) 3016
BmgT120I GGNCC 6 cut(s) 365, 1972, 2141, 3235, 3593, 4358
BmiI GGNNCC 9 cut(s) 768, 1561, 1686, 1890, 2142, 3708, 4005, 4326, 4545
BmrFI CCNGG 6 cut(s) 2006, 2145, 2693, 2948, 3417, 4362
BmtI GCTAGC 2 cut(s) 1113, 2852
BpiI GAAGAC 4 cut(s) 312, 464, 980, 1659
BpmI CTGGAG 4 cut(s) 195, 1029, 1332, 3725
Bpu10I CCTNAGC 6 cut(s) 288, 687, 1736, 2379, 2496, 2643
Bpu14I TTCGAA 2 cut(s) 1364, 3924
BpuEI CTTGAG 4 cut(s) 763, 1943, 2958, 3924
BsaAI YACGTR 1 cut(s) 47
BsaBI GATNNNNATC 2 cut(s) 983, 1851
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 4295
BsaJI CCNNGG 3 cut(s) 2004, 2005, 3606
BsaWI WCCGGW 1 cut(s) 1399
BsaXI ACNNNNNCTCC 8 cut(s) 193, 223, 1305, 1335, 2631, 2661, 3345, 3375
Bsc4I CCNNNNNNNGG 4 cut(s) 218, 1399, 2712, 3529
Bse1I ACTGG 7 cut(s) 212, 433, 582, 1260, 2060, 2405, 3516
Bse21I CCTNAGG 1 cut(s) 1281
Bse3DI GCAATG 2 cut(s) 86, 3228
Bse8I GATNNNNATC 2 cut(s) 983, 1851
BseAI TCCGGA 1 cut(s) 1399
BseBI CCWGG 6 cut(s) 2006, 2145, 2693, 2948, 3417, 4362
BseDI CCNNGG 3 cut(s) 2004, 2005, 3606
BseGI GGATG 6 cut(s) 133, 383, 812, 1146, 1256, 3298
BseJI GATNNNNATC 2 cut(s) 983, 1851
BseLI CCNNNNNNNGG 4 cut(s) 218, 1399, 2712, 3529
BseMI GCAATG 2 cut(s) 86, 3228
BseNI ACTGG 7 cut(s) 212, 433, 582, 1260, 2060, 2405, 3516
BseRI GAGGAG 4 cut(s) 1622, 3336, 4141, 4538
BseSI GKGCMC 2 cut(s) 1138, 4492
BseYI CCCAGC 1 cut(s) 4180
BsgI GTGCAG 1 cut(s) 3009
Bsh1236I CGCG 1 cut(s) 2283
BshFI GGCC 9 cut(s) 546, 1829, 2922, 3236, 3420, 3595, 4360, 4365, 4433
BshNI GGYRCC 2 cut(s) 1559, 1888
BsiHKAI GWGCWC 2 cut(s) 1138, 2036
BsiSI CCGG 1 cut(s) 1400
BsiWI CGTACG 1 cut(s) 4448
BslFI GGGAC 3 cut(s) 1065, 1376, 2225
BslI CCNNNNNNNGG 4 cut(s) 218, 1399, 2712, 3529
BsmAI GTCTC 4 cut(s) 1259, 2945, 3554, 4295
BsmFI GGGAC 3 cut(s) 1065, 1376, 2225
BsmI GAATGC 2 cut(s) 3089, 4120
BsnI GGCC 9 cut(s) 546, 1829, 2922, 3236, 3420, 3595, 4360, 4365, 4433
Bso31I GGTCTC 1 cut(s) 4295
Bsp119I TTCGAA 2 cut(s) 1364, 3924
Bsp1286I GDGCHC 3 cut(s) 1138, 2036, 4492
Bsp13I TCCGGA 1 cut(s) 1399
Bsp1407I TGTACA 2 cut(s) 3295, 4081
Bsp68I TCGCGA 1 cut(s) 2283
BspACI CCGC 8 cut(s) 443, 674, 2698, 2923, 3105, 3116, 3233, 4481
BspANI GGCC 9 cut(s) 546, 1829, 2922, 3236, 3420, 3595, 4360, 4365, 4433
BspEI TCCGGA 1 cut(s) 1399
BspFNI CGCG 1 cut(s) 2283
BspHI TCATGA 2 cut(s) 24, 3714
BspLI GGNNCC 9 cut(s) 768, 1561, 1686, 1890, 2142, 3708, 4005, 4326, 4545
BspOI GCTAGC 2 cut(s) 1113, 2852
BspPI GGATC 8 cut(s) 164, 870, 1731, 1754, 2346, 2892, 2968, 3480
BspQI GCTCTTC 1 cut(s) 1218
BspT104I TTCGAA 2 cut(s) 1364, 3924
BspT107I GGYRCC 2 cut(s) 1559, 1888
BspTI CTTAAG 3 cut(s) 902, 1564, 3788
BspTNI GGTCTC 1 cut(s) 4295
BsrBI CCGCTC 1 cut(s) 2698
BsrDI GCAATG 2 cut(s) 86, 3228
BsrGI TGTACA 2 cut(s) 3295, 4081
BsrI ACTGG 7 cut(s) 212, 433, 582, 1260, 2060, 2405, 3516
BssECI CCNNGG 3 cut(s) 2004, 2005, 3606
BssNAI GTATAC 1 cut(s) 2875
BssNI GRCGYC 1 cut(s) 29
BssSI CACGAG 3 cut(s) 747, 1076, 1125
BssT1I CCWWGG 1 cut(s) 3606
Bst1107I GTATAC 1 cut(s) 2875
Bst2BI CACGAG 3 cut(s) 747, 1076, 1125
Bst2UI CCWGG 6 cut(s) 2006, 2145, 2693, 2948, 3417, 4362
Bst6I CTCTTC 5 cut(s) 953, 1041, 1218, 1630, 1821
BstACI GRCGYC 1 cut(s) 29
BstAFI CTTAAG 3 cut(s) 902, 1564, 3788
BstAPI GCANNNNNTGC 2 cut(s) 2425, 4475
BstAUI TGTACA 2 cut(s) 3295, 4081
BstBAI YACGTR 1 cut(s) 47
BstBI TTCGAA 2 cut(s) 1364, 3924
BstC8I GCNNGC 9 cut(s) 398, 1111, 1932, 2507, 2778, 2850, 3193, 3220, 4476
BstEII GGTNACC 1 cut(s) 3032
BstENI CCTNNNNNAGG 1 cut(s) 3527
BstF5I GGATG 6 cut(s) 133, 383, 812, 1146, 1256, 3298
BstFNI CGCG 1 cut(s) 2283
BstH2I RGCGCY 1 cut(s) 1743
BstHHI GCGC 1 cut(s) 1742
BstMAI GTCTC 4 cut(s) 1259, 2945, 3554, 4295
BstNI CCWGG 6 cut(s) 2006, 2145, 2693, 2948, 3417, 4362
BstNSI RCATGY 6 cut(s) 400, 1527, 2615, 2780, 3008, 4478
BstPI GGTNACC 1 cut(s) 3032
BstSCI CCNGG 6 cut(s) 2004, 2143, 2691, 2946, 3415, 4360
BstSFI CTRYAG 3 cut(s) 1783, 2296, 2955
BstSLI GKGCMC 2 cut(s) 1138, 4492
BstSNI TACGTA 1 cut(s) 47
BstUI CGCG 1 cut(s) 2283
BstV2I GAAGAC 4 cut(s) 312, 464, 980, 1659
BstX2I RGATCY 6 cut(s) 756, 875, 1723, 1868, 1923, 2960
BstXI CCANNNNNNTGG 2 cut(s) 362, 3613
BstYI RGATCY 6 cut(s) 756, 875, 1723, 1868, 1923, 2960
BstZ17I GTATAC 1 cut(s) 2875
Bsu36I CCTNAGG 1 cut(s) 1281
BsuI GTATCC 2 cut(s) 3411, 4411
BsuRI GGCC 9 cut(s) 546, 1829, 2922, 3236, 3420, 3595, 4360, 4365, 4433
BtrI CACGTC 1 cut(s) 3016
BtsCI GGATG 6 cut(s) 133, 383, 812, 1146, 1256, 3298
BtsI GCAGTG 2 cut(s) 2992, 4147
BtuMI TCGCGA 1 cut(s) 2283
Cac8I GCNNGC 9 cut(s) 398, 1111, 1932, 2507, 2778, 2850, 3193, 3220, 4476
CaiI CAGNNNCTG 2 cut(s) 393, 1103
CciI TCATGA 2 cut(s) 24, 3714
CfoI GCGC 1 cut(s) 1742
Cfr13I GGNCC 6 cut(s) 365, 1972, 2141, 3235, 3593, 4358
CseI GACGC 1 cut(s) 37
Csp6I GTAC 8 cut(s) 486, 708, 1485, 3296, 3519, 4082, 4449, 4498
CviQI GTAC 8 cut(s) 486, 708, 1485, 3296, 3519, 4082, 4449, 4498
DraI TTTAAA 1 cut(s) 3112
EaeI YGGCCR 1 cut(s) 3418
Eam1104I CTCTTC 5 cut(s) 953, 1041, 1218, 1630, 1821
EarI CTCTTC 5 cut(s) 953, 1041, 1218, 1630, 1821
Eco105I TACGTA 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 3606
Eco147I AGGCCT 1 cut(s) 4433
Eco31I GGTCTC 1 cut(s) 4295
Eco47I GGWCC 3 cut(s) 365, 1972, 2141
Eco47III AGCGCT 1 cut(s) 1741
Eco57I CTGAAG 5 cut(s) 802, 977, 1195, 2531, 2627
Eco81I CCTNAGG 1 cut(s) 1281
Eco91I GGTNACC 1 cut(s) 3032
EcoNI CCTNNNNNAGG 1 cut(s) 3527
EcoO65I GGTNACC 1 cut(s) 3032
EcoRI GAATTC 2 cut(s) 804, 1403
EcoRII CCWGG 6 cut(s) 2004, 2143, 2691, 2946, 3415, 4360
EcoT14I CCWWGG 1 cut(s) 3606
EcoT22I ATGCAT 5 cut(s) 845, 1473, 1525, 3188, 3781
ErhI CCWWGG 1 cut(s) 3606
FalI AAGNNNNNCTT 4 cut(s) 1800, 1832, 4476, 4508
FaqI GGGAC 3 cut(s) 1065, 1376, 2225
FbaI TGATCA 2 cut(s) 3375, 4531
FblI GTMKAC 1 cut(s) 2874
FokI GGATG 6 cut(s) 120, 370, 799, 1153, 1243, 3285
GlaI GCGC 1 cut(s) 1741
GsaI CCCAGC 1 cut(s) 4184
GsuI CTGGAG 4 cut(s) 195, 1029, 1332, 3725
HaeII RGCGCY 1 cut(s) 1743
HaeIII GGCC 9 cut(s) 546, 1829, 2922, 3236, 3420, 3595, 4360, 4365, 4433
HapII CCGG 1 cut(s) 1400
HgaI GACGC 1 cut(s) 37
HhaI GCGC 1 cut(s) 1742
Hin1I GRCGYC 1 cut(s) 29
Hin6I GCGC 1 cut(s) 1740
HinP1I GCGC 1 cut(s) 1740
HincII GTYRAC 1 cut(s) 3100
HindII GTYRAC 1 cut(s) 3100
HindIII AAGCTT 1 cut(s) 1367
HpaII CCGG 1 cut(s) 1400
HphI GGTGA 8 cut(s) 599, 1055, 1430, 1723, 2084, 2807, 3026, 3370
HpyCH4IV ACGT 7 cut(s) 46, 351, 793, 2812, 3015, 3929, 4165
HpySE526I ACGT 7 cut(s) 46, 351, 793, 2812, 3015, 3929, 4165
Hsp92I GRCGYC 1 cut(s) 29
HspAI GCGC 1 cut(s) 1740
Kpn2I TCCGGA 1 cut(s) 1399
Ksp22I TGATCA 2 cut(s) 3375, 4531
LguI GCTCTTC 1 cut(s) 1218
LmnI GCTCC 8 cut(s) 766, 1313, 1468, 2433, 2639, 2695, 3086, 3706
MaeII ACGT 7 cut(s) 46, 351, 793, 2812, 3015, 3929, 4165
MbiI CCGCTC 1 cut(s) 2698
MfeI CAATTG 2 cut(s) 3057, 3441
MflI RGATCY 6 cut(s) 756, 875, 1723, 1868, 1923, 2960
MhlI GDGCHC 3 cut(s) 1138, 2036, 4492
MlsI TGGCCA 1 cut(s) 3420
MluNI TGGCCA 1 cut(s) 3420
MlyI GAGTC 6 cut(s) 656, 951, 1616, 1619, 1712, 3638
MmeI TCCRAC 7 cut(s) 1173, 1698, 2734, 3064, 3750, 3981, 4131
Mox20I TGGCCA 1 cut(s) 3420
Mph1103I ATGCAT 5 cut(s) 845, 1473, 1525, 3188, 3781
MroI TCCGGA 1 cut(s) 1399
MroXI GAANNNNTTC 2 cut(s) 808, 1998
MscI TGGCCA 1 cut(s) 3420
MslI CAYNNNNRTG 2 cut(s) 138, 360
Msp20I TGGCCA 1 cut(s) 3420
MspA1I CMGCKG 4 cut(s) 424, 695, 3105, 4481
MspCI CTTAAG 3 cut(s) 902, 1564, 3788
MspI CCGG 1 cut(s) 1400
MspR9I CCNGG 6 cut(s) 2006, 2145, 2693, 2948, 3417, 4362
MunI CAATTG 2 cut(s) 3057, 3441
Mva1269I GAATGC 2 cut(s) 3089, 4120
MvaI CCWGG 6 cut(s) 2006, 2145, 2693, 2948, 3417, 4362
MvnI CGCG 1 cut(s) 2283
NheI GCTAGC 2 cut(s) 1109, 2848
NlaIV GGNNCC 9 cut(s) 768, 1561, 1686, 1890, 2142, 3708, 4005, 4326, 4545
NmeAIII GCCGAG 1 cut(s) 2682
NmuCI GTSAC 9 cut(s) 464, 1122, 1321, 1568, 1729, 2942, 3000, 3011, 3032
NruI TCGCGA 1 cut(s) 2283
NsiI ATGCAT 5 cut(s) 845, 1473, 1525, 3188, 3781
NspI RCATGY 6 cut(s) 400, 1527, 2615, 2780, 3008, 4478
NspV TTCGAA 2 cut(s) 1364, 3924
PaeI GCATGC 3 cut(s) 400, 2780, 4478
PagI TCATGA 2 cut(s) 24, 3714
PasI CCCWGGG 1 cut(s) 2005
PceI AGGCCT 1 cut(s) 4433
PciSI GCTCTTC 1 cut(s) 1218
PctI GAATGC 2 cut(s) 3089, 4120
PdmI GAANNNNTTC 2 cut(s) 808, 1998
PfeI GAWTC 5 cut(s) 907, 1018, 1779, 2450, 2969
Pfl23II CGTACG 1 cut(s) 4448
PfoI TCCNGGA 1 cut(s) 2143
PleI GAGTC 6 cut(s) 656, 950, 1616, 1618, 1711, 3637
PpsI GAGTC 6 cut(s) 656, 950, 1616, 1618, 1711, 3637
Ppu21I YACGTR 1 cut(s) 47
PshBI ATTAAT 1 cut(s) 611
Psp1406I AACGTT 3 cut(s) 2812, 3929, 4165
Psp6I CCWGG 6 cut(s) 2004, 2143, 2691, 2946, 3415, 4360
PspEI GGTNACC 1 cut(s) 3032
PspFI CCCAGC 1 cut(s) 4180
PspGI CCWGG 6 cut(s) 2004, 2143, 2691, 2946, 3415, 4360
PspLI CGTACG 1 cut(s) 4448
PspN4I GGNNCC 9 cut(s) 768, 1561, 1686, 1890, 2142, 3708, 4005, 4326, 4545
PspPI GGNCC 6 cut(s) 365, 1972, 2141, 3235, 3593, 4358
PstNI CAGNNNCTG 2 cut(s) 393, 1103
PsuI RGATCY 6 cut(s) 756, 875, 1723, 1868, 1923, 2960
PvuII CAGCTG 2 cut(s) 424, 695
RruI TCGCGA 1 cut(s) 2283
RsaI GTAC 8 cut(s) 487, 709, 1486, 3297, 3520, 4083, 4450, 4499
RsaNI GTAC 8 cut(s) 486, 708, 1485, 3296, 3519, 4082, 4449, 4498
RseI CAYNNNNRTG 2 cut(s) 138, 360
SapI GCTCTTC 1 cut(s) 1218
Sau96I GGNCC 6 cut(s) 365, 1972, 2141, 3235, 3593, 4358
SchI GAGTC 6 cut(s) 656, 951, 1616, 1619, 1712, 3638
ScrFI CCNGG 6 cut(s) 2006, 2145, 2693, 2948, 3417, 4362
SduI GDGCHC 3 cut(s) 1138, 2036, 4492
SfcI CTRYAG 3 cut(s) 1783, 2296, 2955
SfuI TTCGAA 2 cut(s) 1364, 3924
SinI GGWCC 3 cut(s) 365, 1972, 2141
SmiMI CAYNNNNRTG 2 cut(s) 138, 360
SmlI CTYRAG 7 cut(s) 778, 902, 1564, 1958, 2973, 3788, 3939
SmoI CTYRAG 7 cut(s) 778, 902, 1564, 1958, 2973, 3788, 3939
SnaBI TACGTA 1 cut(s) 47
SphI GCATGC 3 cut(s) 400, 2780, 4478
SseBI AGGCCT 1 cut(s) 4433
SsiI CCGC 8 cut(s) 443, 674, 2698, 2923, 3105, 3116, 3233, 4481
SspI AATATT 3 cut(s) 525, 2133, 2938
StuI AGGCCT 1 cut(s) 4433
StyD4I CCNGG 6 cut(s) 2004, 2143, 2691, 2946, 3415, 4360
StyI CCWWGG 1 cut(s) 3606
TaiI ACGT 7 cut(s) 49, 354, 796, 2815, 3018, 3932, 4168
TaqII GACCGA 2 cut(s) 993, 1989
TatI WGTACW 4 cut(s) 485, 1484, 3295, 4081
TauI GCSGC 3 cut(s) 2925, 3119, 3236
TfiI GAWTC 5 cut(s) 907, 1018, 1779, 2450, 2969
TseFI GTSAC 9 cut(s) 464, 1122, 1321, 1568, 1729, 2942, 3000, 3011, 3032
Tsp45I GTSAC 9 cut(s) 464, 1122, 1321, 1568, 1729, 2942, 3000, 3011, 3032
TspGWI ACGGA 2 cut(s) 1153, 1309
Vha464I CTTAAG 3 cut(s) 902, 1564, 3788
VneI GTGCAC 1 cut(s) 1134
VpaK11BI GGWCC 3 cut(s) 365, 1972, 2141
VspI ATTAAT 1 cut(s) 611
XagI CCTNNNNNAGG 1 cut(s) 3527
XbaI TCTAGA 2 cut(s) 407, 2665
XceI RCATGY 6 cut(s) 400, 1527, 2615, 2780, 3008, 4478
XmiI GTMKAC 1 cut(s) 2874
XmnI GAANNNNTTC 2 cut(s) 808, 1998
Zsp2I ATGCAT 5 cut(s) 845, 1473, 1525, 3188, 3781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.