Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007461

Physical Location & Seq

Genomic Coordinates Forward (+)

7446962 .. 7451049

Gene Structure 4088 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G22470.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2448 bp

ATGAAGGTTCACGAGACAAGATCTCACGCTCACATGTCTGGAGACGAACAAAAGAAGGGAAATTTGCGGAAGCACAAAGCAGAAGGGAAACTTCCAGAATCTGAACAGTCTCAGAAGAAGGCAAAGCCTGAAAACGATGACGGACGTTCTGTCAACGGCGCCGGAGATGCTGCTTCAGAGTACAATGAGTTCTGCAAAGCGGTTGAGGAGAATCTGTCCATTGATCAGATTAAAGAAGTTCTCGAAATCAACGGCCAAGATTGTTCTGCTCCAGAAGAGACCTTGCTAGCTCAATGTCAAGATTTGCTGTTCTATGGGGCATTAGCTAAATGTCCTTTATGCGGAGGAACTTTAATTTGCGACAATGAAAAGAGATTTGTATGTGGAGGTGAGATAAGTGAGTGGTGCAGTTGCGTGTTTAGTACGAAAGATCCTCCTAGAAAGGAAGAGCCAGTTAAAATCCCTGATTCTGTCATGAACTCTGCTATATCTGACTTGATCAAGAAACACCAGGACCCTAAAAGCCGACCTAAAAGAGAGTTAGGCTCTGCTGATAAACCCTTTGTGGGAATGATGATCTCTCTCATGGGACGTCTCACGAGAACACATCAATATTGGAAGAAAAAGATCGAGAGAAACGGTGGGAAAGTCTCCAATACTGTTCAAGGCGTAACATGTTTGGTGGTTTCGCCAGCTGAAAGAGAACGAGGTGGTACGTCAAAGATGGTGGAGGCAATGGAACAAGGTCTACCGGTTGTGAGCGAAGCATGGTTGATCGACAGCGTGGAGAAGCATGAAGCTCAGCCACTTGAAGCTTATGACGTGGTCAGTGATCTTTCAGTGGAAGGGAAAGGAATTCCATGGGATAAGCAAGATCCTAGTGAGGAGGCAATTGAATCCTTTTCTGCTGAGCTCAAAATGTATGGGAAAAGAGGAGTGTACATGGACACAAAACTTCAGGAGAGAGGAGGAAAGATCTTCGAGAAAGATGGACTCTTGTATAACTGTGCCTTCTCGATATGCGATTTGGGAAAAGGGCGTAATGAGTATTGTATTATGCAGCTAGTCACGGTACCCGATAGTAACCTGAACATGTACTTCAAGAGAGGGAAAGTAGGAGATGACCCTAATGCCGAAGAGAGGCTCGAGGAATGGGAGGACGAAGAAGCTGCGATCAAAGAGTTTGCAAGGCTTTTTGAGGAGATAGCAGGGAATGAGTTTGAGCCATGGGAACGTGAGAAGAAGATTCAAAAGAAGCCTCATAAGTTTTTCCCAATTGATATGGATGATGGAATCGAAGTAAGGAGTGGGGCTCTTGGTCTAAGGCAGCTTGGCATTGCTTCTGCTCATTGCAAGCTTGATTCGTTTGTTGCAAACTTCATTAAAGTTCTGTGTGGTCAAGAGATTTACAATTACGCGTTGATGGAGCTTGGATTGGATCCGCCCGATCTACCTATGGGAATGCTAACTGATATCCACTTGAAACGATGCGAAGAGGTATTACTCGAGTTTGTTGAGAAGGTCAAAACAACAAAAGAGACAGGTCAGAAAGCTGAAGCAATGTGGGCAGACTTCAGCTCACGATGGTTCTCTTTGATGCACAGCACTAGGCCGATGCGATTACACGATGTCAATGAACTTGCAGACCATGCGGCCTCTGCTTTTGAGACGGTGAGGGACATAAACACAGCATCTCGTTTGATAGGGGACATGCGAGGAGACACACTCGATGATCCGTTGTCTGATAGGTACAAAAAACTTGGCTGCAAGATATCTGTGGTAGACAAAGAGTCTGAAGATTACAAGATGGTTGTGAAGTATCTCGAGACTACTTATGAGCCTGTGAAAGTCTCTGATGTTGAGTACGGTGTGTCAGTGCAGAATGTTTTTGCGGTTGAGTCAGATGCAATTCCTTCATTAGATGATATCAAGAAGTTACCAAATAAGGTCCTTTTATGGTGTGGGTCTCGGAGCTCAAATCTATTGAGACATATCTACAAAGGGTTCTTACCTGCTGTATGCTCTCTTCCGGTTCCTGGTTATATGTTTGGGAGAGCGATAGTGTGTTCAGATGCAGCTGCAGAAGCAGCAAGGTATGGTTTTACGGCTGTGGATAGACCAGAAGGGTTTCTTGTATTAGCCGTAGCATCACTTGGTGAGGAAGTTACAGAATTTACAAGTCCACCAGAGGATACGAAGACGTTGGAAGATAAAAAGATTGGAGTGAAAGGATTAGGGAGGAAGAAAACTGAAGAGTCGGAGCATTTCATGTGGAGAGATGACATAAAAGTTCCTTGTGGACGGTTGGTTCCATCGGAACATAAGGACAGTCCACTTGAGTACAACGAGTACGCGGTTTATGATCCGAAACAGACAAGTATAAGGTTCTTGGTGGAAGTGAAGTACGAGGAGAAGGGAACTGAGATAGTCGATGTCGAACCAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016874 GO:0016886 GO:0022616 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051103 GO:0051716 GO:0071704 GO:0090304 GO:0140097 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03410 ko04210 ko04212 ko04214 ko04217 map03410 map04210 map04212 map04214 map04217

Pfam Domains

Protein Families

BRCT BRCT_2 PADR1 PARP PARP_reg WGR

Protein Analysis

815

Amino Acids

91.53

Weight (kDa)

5.15

Isoelectric Point (pI)

41.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PADR1_N	PF21728	58 - 106	7e-13	PADR1, N-terminal helical domain
Zn_ribbon_PADR1	PF08063	107 - 148	1e-12	PADR1 domain, zinc ribbon fold
BRCT	PF00533	185 - 261	1.6e-08	BRCA1 C Terminus (BRCT) domain
BRCT_2	PF16589	188 - 274	1.3e-07	BRCT domain
WGR	PF05406	334 - 412	1.3e-11	WGR domain
PARP_reg	PF02877	451 - 584	3.7e-22	Poly(ADP-ribose) polymerase, regulatory domain
PARP	PF00644	598 - 801	3.2e-52	Poly(ADP-ribose) polymerase catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22470
fragaria_vesca	FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670
malus_domestica	MD03G1142500.v1.1
prunus_persica	Prupe.6G127600_v2.0.a1 Prupe.6G127600_v2.0.a1 Prupe.6G127600_v2.0.a1 Prupe.6G127600_v2.0.a1
pyrus_communis	pycom03g09710
rosa_chinensis	RchiOBHm_Chr5g0044231
rosa_laevigata	RLG00000034257
rosa_multiflora	Rmu_sc0000776.1_g000071
rosa_roxburghii	Rroxscaffold_1G00036790
rosa_rugosa	Rorug05G0212700
rosa_samantha	Rh5AG296800 Rh5BG304300 Rh5CG331300 Rh5DG313100
rosa_wichuraiana	Rw5G027490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	595
Acc36I	ACCTGC	1 cut(s)	2020
Acc65I	GGTACC	1 cut(s)	1072
AccB1I	GGYRCC	2 cut(s)	158, 1072
AccI	GTMKAC	2 cut(s)	748, 1782
AccII	CGCG	2 cut(s)	1418, 2354
AciI	CCGC	7 cut(s)	67, 200, 342, 1442, 1652, 1892, 2354
AclWI	GGATC	6 cut(s)	425, 869, 1433, 1446, 1727, 2357
AcoI	YGGCCR	1 cut(s)	253
AcsI	RAATTY	3 cut(s)	61, 855, 2171
AcuI	CTGAAG	6 cut(s)	159, 941, 1558, 1575, 1815, 2271
AcyI	GRCGYC	2 cut(s)	159, 592
AdeI	CACNNNGTG	1 cut(s)	2156
AfiI	CCNNNNNNNGG	4 cut(s)	341, 566, 1140, 2036
AflIII	ACRYGT	4 cut(s)	33, 674, 1092, 1416
AgeI	ACCGGT	1 cut(s)	751
AgsI	TTSAA	6 cut(s)	665, 812, 896, 1102, 1250, 1483
AhdI	GACNNNNNGTC	2 cut(s)	149, 1789
AjiI	CACGTC	1 cut(s)	823
AjnI	CCWGG	2 cut(s)	510, 2035
AloI	GAACNNNNNNTCC	2 cut(s)	2292, 2324
Alw21I	GWGCWC	2 cut(s)	915, 1976
AlwI	GGATC	6 cut(s)	425, 869, 1433, 1446, 1727, 2357
AlwNI	CAGNNNCTG	1 cut(s)	101
Ama87I	CYCGRG	3 cut(s)	1145, 1505, 1823
AoxI	GGCC	3 cut(s)	253, 1610, 1653
ApeKI	GCWGC	8 cut(s)	170, 1060, 1169, 1327, 1764, 2075, 2078, 2087
ApoI	RAATTY	3 cut(s)	61, 855, 2171
AsiGI	ACCGGT	1 cut(s)	751
Asp700I	GAANNNNTTC	1 cut(s)	2127
Asp718I	GGTACC	1 cut(s)	1072
AspLEI	GCGC	1 cut(s)	161
AspS9I	GGNCC	2 cut(s)	514, 1948
AsuHPI	GGTGA	3 cut(s)	401, 1684, 2168
AsuNHI	GCTAGC	1 cut(s)	286
AvaI	CYCGRG	3 cut(s)	1145, 1505, 1823
AvaII	GGWCC	2 cut(s)	514, 1948
BamHI	GGATCC	1 cut(s)	1438
BanI	GGYRCC	2 cut(s)	158, 1072
BanII	GRGCYC	3 cut(s)	915, 1315, 1976
BarI	GAAGNNNNNNTAC	4 cut(s)	1485, 1517, 2010, 2042
BauI	CACGAG	2 cut(s)	11, 598
BbsI	GAAGAC	1 cut(s)	2204
Bbv12I	GWGCWC	2 cut(s)	915, 1976
BbvI	GCAGC	8 cut(s)	157, 1072, 1156, 1339, 1751, 2065, 2087, 2099
BccI	CCATC	7 cut(s)	718, 983, 1283, 1417, 1578, 1801, 2320
BceAI	ACGGC	4 cut(s)	172, 268, 2121, 2126
BcgI	CGANNNNNNTGC	2 cut(s)	1151, 1185
BciT130I	CCWGG	2 cut(s)	512, 2037
BciVI	GTATCC	1 cut(s)	2185
BclI	TGATCA	2 cut(s)	223, 498
BfaI	CTAG	5 cut(s)	287, 438, 879, 1064, 1608
BfmI	CTRYAG	1 cut(s)	2079
BfoI	RGCGCY	1 cut(s)	162
BfuAI	ACCTGC	1 cut(s)	2020
BfuI	GTATCC	1 cut(s)	2185
BglII	AGATCT	2 cut(s)	20, 975
BisI	GCNGC	9 cut(s)	171, 1061, 1170, 1328, 1653, 1765, 2076, 2079, 2088
BlpI	GCTNAGC	2 cut(s)	801, 909
BlsI	GCNGC	9 cut(s)	172, 1062, 1171, 1329, 1654, 1766, 2077, 2080, 2089
Bme1390I	CCNGG	2 cut(s)	512, 2037
Bme18I	GGWCC	2 cut(s)	514, 1948
BmeRI	GACNNNNNGTC	2 cut(s)	149, 1789
BmeT110I	CYCGRG	3 cut(s)	1145, 1505, 1823
BmgBI	CACGTC	1 cut(s)	823
BmgT120I	GGNCC	2 cut(s)	514, 1948
BmiI	GGNNCC	6 cut(s)	160, 516, 1074, 1440, 2034, 2310
BmrFI	CCNGG	2 cut(s)	512, 2037
BmsI	GCATC	8 cut(s)	157, 1478, 1587, 1605, 1700, 1894, 2062, 2156
BmtI	GCTAGC	1 cut(s)	290
BpiI	GAAGAC	1 cut(s)	2204
BplI	GAGNNNNNCTC	6 cut(s)	530, 562, 1297, 1329, 1710, 1742
BpmI	CTGGAG	2 cut(s)	60, 255
Bpu1102I	GCTNAGC	2 cut(s)	801, 909
BpuEI	CTTGAG	1 cut(s)	2357
BsaHI	GRCGYC	2 cut(s)	159, 592
BsaI	GGTCTC	2 cut(s)	272, 1971
BsaJI	CCNNGG	2 cut(s)	860, 1226
BsaWI	WCCGGW	2 cut(s)	751, 2029
BsaXI	ACNNNNNCTCC	2 cut(s)	200, 230
Bsc4I	CCNNNNNNNGG	4 cut(s)	341, 566, 1140, 2036
Bse118I	RCCGGY	1 cut(s)	751
Bse1I	ACTGG	1 cut(s)	452
Bse3DI	GCAATG	4 cut(s)	741, 1335, 1348, 1566
BseBI	CCWGG	2 cut(s)	512, 2037
BseDI	CCNNGG	2 cut(s)	860, 1226
BseGI	GGATG	1 cut(s)	1291
BseLI	CCNNNNNNNGG	4 cut(s)	341, 566, 1140, 2036
BseMI	GCAATG	4 cut(s)	741, 1335, 1348, 1566
BseMII	CTCAG	4 cut(s)	125, 815, 900, 2412
BseNI	ACTGG	1 cut(s)	452
BseRI	GAGGAG	7 cut(s)	221, 899, 948, 981, 1214, 1731, 2423
BseXI	GCAGC	8 cut(s)	157, 1072, 1156, 1339, 1751, 2065, 2087, 2099
BsgI	GTGCAG	2 cut(s)	427, 1898
Bsh1236I	CGCG	2 cut(s)	1418, 2354
BshFI	GGCC	3 cut(s)	255, 1612, 1655
BshNI	GGYRCC	2 cut(s)	158, 1072
BshTI	ACCGGT	1 cut(s)	751
BsiHKAI	GWGCWC	2 cut(s)	915, 1976
BsiHKCI	CYCGRG	3 cut(s)	1145, 1505, 1823
BsiSI	CCGG	3 cut(s)	162, 752, 2030
BslFI	GGGAC	3 cut(s)	603, 1691, 1721
BslI	CCNNNNNNNGG	4 cut(s)	341, 566, 1140, 2036
BsmBI	CGTCTC	3 cut(s)	36, 599, 1661
BsmFI	GGGAC	3 cut(s)	603, 1691, 1721
BsmI	GAATGC	1 cut(s)	1467
BsnI	GGCC	3 cut(s)	255, 1612, 1655
Bso31I	GGTCTC	2 cut(s)	272, 1971
BsoBI	CYCGRG	3 cut(s)	1145, 1505, 1823
Bsp1286I	GDGCHC	3 cut(s)	915, 1315, 1976
Bsp1407I	TGTACA	1 cut(s)	939
Bsp1720I	GCTNAGC	2 cut(s)	801, 909
Bsp19I	CCATGG	2 cut(s)	860, 1226
BspACI	CCGC	7 cut(s)	67, 200, 342, 1442, 1652, 1892, 2354
BspANI	GGCC	3 cut(s)	255, 1612, 1655
BspCNI	CTCAG	4 cut(s)	124, 814, 901, 2413
BspFNI	CGCG	2 cut(s)	1418, 2354
BspHI	TCATGA	1 cut(s)	474
BspLI	GGNNCC	6 cut(s)	160, 516, 1074, 1440, 2034, 2310
BspMAI	CTGCAG	1 cut(s)	2083
BspMI	ACCTGC	1 cut(s)	2020
BspOI	GCTAGC	1 cut(s)	290
BspPI	GGATC	6 cut(s)	425, 869, 1433, 1446, 1727, 2357
BspQI	GCTCTTC	1 cut(s)	441
BspT107I	GGYRCC	2 cut(s)	158, 1072
BspTNI	GGTCTC	2 cut(s)	272, 1971
BsrDI	GCAATG	4 cut(s)	741, 1335, 1348, 1566
BsrFI	RCCGGY	1 cut(s)	751
BsrGI	TGTACA	1 cut(s)	939
BsrI	ACTGG	1 cut(s)	452
BssAI	RCCGGY	1 cut(s)	751
BssECI	CCNNGG	2 cut(s)	860, 1226
BssNI	GRCGYC	2 cut(s)	159, 592
BssSI	CACGAG	2 cut(s)	11, 598
BssT1I	CCWWGG	2 cut(s)	860, 1226
Bst2BI	CACGAG	2 cut(s)	11, 598
Bst2UI	CCWGG	2 cut(s)	512, 2037
Bst4CI	ACNGT	9 cut(s)	108, 641, 661, 1007, 1072, 1672, 1868, 2304, 2330
Bst6I	CTCTTC	6 cut(s)	270, 441, 1131, 1488, 2031, 2247
BstACI	GRCGYC	2 cut(s)	159, 592
BstAPI	GCANNNNNTGC	1 cut(s)	1649
BstAUI	TGTACA	1 cut(s)	939
BstC8I	GCNNGC	3 cut(s)	288, 693, 1355
BstDEI	CTNAG	5 cut(s)	111, 801, 909, 1322, 2421
BstDSI	CCRYGG	2 cut(s)	860, 1226
BstF5I	GGATG	1 cut(s)	1291
BstFNI	CGCG	2 cut(s)	1418, 2354
BstH2I	RGCGCY	1 cut(s)	162
BstHHI	GCGC	1 cut(s)	161
BstMWI	GCNNNNNNNGC	6 cut(s)	167, 1565, 1649, 1658, 2084, 2087
BstNI	CCWGG	2 cut(s)	512, 2037
BstNSI	RCATGY	4 cut(s)	37, 678, 1096, 1714
BstSCI	CCNGG	2 cut(s)	510, 2035
BstSFI	CTRYAG	1 cut(s)	2079
BstUI	CGCG	2 cut(s)	1418, 2354
BstV1I	GCAGC	8 cut(s)	157, 1072, 1156, 1339, 1751, 2065, 2087, 2099
BstV2I	GAAGAC	1 cut(s)	2204
BstX2I	RGATCY	5 cut(s)	20, 430, 874, 975, 1438
BstYI	RGATCY	5 cut(s)	20, 430, 874, 975, 1438
BsuI	GTATCC	1 cut(s)	2185
BsuRI	GGCC	3 cut(s)	255, 1612, 1655
BtgI	CCRYGG	2 cut(s)	860, 1226
BtrI	CACGTC	1 cut(s)	823
BtsCI	GGATG	1 cut(s)	1291
BtsIMutI	CAGTG	3 cut(s)	835, 846, 1881
BveI	ACCTGC	1 cut(s)	2020
Cac8I	GCNNGC	3 cut(s)	288, 693, 1355
CaiI	CAGNNNCTG	1 cut(s)	101
CciI	TCATGA	1 cut(s)	474
CfoI	GCGC	1 cut(s)	161
Cfr10I	RCCGGY	1 cut(s)	751
Cfr13I	GGNCC	2 cut(s)	514, 1948
CspAI	ACCGGT	1 cut(s)	751
CspCI	CAANNNNNGTGG	2 cut(s)	708, 743
DdeI	CTNAG	5 cut(s)	111, 801, 909, 1322, 2421
DinI	GGCGCC	1 cut(s)	160
DraIII	CACNNNGTG	1 cut(s)	2156
DriI	GACNNNNNGTC	2 cut(s)	149, 1789
EaeI	YGGCCR	1 cut(s)	253
Eam1104I	CTCTTC	6 cut(s)	270, 441, 1131, 1488, 2031, 2247
Eam1105I	GACNNNNNGTC	2 cut(s)	149, 1789
EarI	CTCTTC	6 cut(s)	270, 441, 1131, 1488, 2031, 2247
EciI	GGCGGA	1 cut(s)	1431
Ecl136II	GAGCTC	2 cut(s)	913, 1974
Eco130I	CCWWGG	2 cut(s)	860, 1226
Eco24I	GRGCYC	3 cut(s)	915, 1315, 1976
Eco31I	GGTCTC	2 cut(s)	272, 1971
Eco32I	GATATC	3 cut(s)	1474, 1773, 1927
Eco47I	GGWCC	2 cut(s)	514, 1948
Eco53kI	GAGCTC	2 cut(s)	913, 1974
Eco57I	CTGAAG	6 cut(s)	159, 941, 1558, 1575, 1815, 2271
Eco88I	CYCGRG	3 cut(s)	1145, 1505, 1823
EcoICRI	GAGCTC	2 cut(s)	913, 1974
EcoO109I	RGGNCCY	2 cut(s)	514, 1948
EcoRI	GAATTC	1 cut(s)	855
EcoRII	CCWGG	2 cut(s)	510, 2035
EcoRV	GATATC	3 cut(s)	1474, 1773, 1927
EcoT14I	CCWWGG	2 cut(s)	860, 1226
EcoT38I	GRGCYC	3 cut(s)	915, 1315, 1976
EgeI	GGCGCC	1 cut(s)	160
EheI	GGCGCC	1 cut(s)	160
ErhI	CCWWGG	2 cut(s)	860, 1226
Esp3I	CGTCTC	3 cut(s)	36, 599, 1661
FalI	AAGNNNNNCTT	4 cut(s)	75, 107, 2115, 2147
FaqI	GGGAC	3 cut(s)	603, 1691, 1721
FbaI	TGATCA	2 cut(s)	223, 498
FblI	GTMKAC	2 cut(s)	748, 1782
Fnu4HI	GCNGC	9 cut(s)	171, 1061, 1170, 1328, 1653, 1765, 2076, 2079, 2088
FokI	GGATG	1 cut(s)	1298
FriOI	GRGCYC	3 cut(s)	915, 1315, 1976
Fsp4HI	GCNGC	9 cut(s)	171, 1061, 1170, 1328, 1653, 1765, 2076, 2079, 2088
FspBI	CTAG	5 cut(s)	287, 438, 879, 1064, 1608
GlaI	GCGC	1 cut(s)	160
GluI	GCNGC	9 cut(s)	171, 1061, 1170, 1328, 1653, 1765, 2076, 2079, 2088
GsuI	CTGGAG	2 cut(s)	60, 255
HaeII	RGCGCY	1 cut(s)	162
HaeIII	GGCC	3 cut(s)	255, 1612, 1655
HapII	CCGG	3 cut(s)	162, 752, 2030
HhaI	GCGC	1 cut(s)	161
Hin1I	GRCGYC	2 cut(s)	159, 592
Hin6I	GCGC	1 cut(s)	159
HinP1I	GCGC	1 cut(s)	159
HincII	GTYRAC	1 cut(s)	154
HindII	GTYRAC	1 cut(s)	154
HindIII	AAGCTT	2 cut(s)	813, 1355
HpaII	CCGG	3 cut(s)	162, 752, 2030
HphI	GGTGA	3 cut(s)	401, 1684, 2168
Hpy166II	GTNNAC	8 cut(s)	10, 154, 749, 940, 1783, 2183, 2300, 2333
Hpy8I	GTNNAC	8 cut(s)	10, 154, 749, 940, 1783, 2183, 2300, 2333
HpyAV	CCTTC	9 cut(s)	49, 77, 112, 839, 1021, 1513, 1923, 2117, 2407
HpyCH4III	ACNGT	9 cut(s)	108, 641, 661, 1007, 1072, 1672, 1868, 2304, 2330
HpyCH4IV	ACGT	6 cut(s)	145, 592, 716, 822, 1234, 2201
HpyF10VI	GCNNNNNNNGC	6 cut(s)	167, 1565, 1649, 1658, 2084, 2087
HpyF3I	CTNAG	5 cut(s)	111, 801, 909, 1322, 2421
HpySE526I	ACGT	6 cut(s)	145, 592, 716, 822, 1234, 2201
Hsp92I	GRCGYC	2 cut(s)	159, 592
HspAI	GCGC	1 cut(s)	159
KasI	GGCGCC	1 cut(s)	158
KpnI	GGTACC	1 cut(s)	1076
Ksp22I	TGATCA	2 cut(s)	223, 498
LguI	GCTCTTC	1 cut(s)	441
LmnI	GCTCC	4 cut(s)	274, 1426, 1971, 2260
Lsp1109I	GCAGC	8 cut(s)	157, 1072, 1156, 1339, 1751, 2065, 2087, 2099
LweI	GCATC	8 cut(s)	157, 1478, 1587, 1605, 1700, 1894, 2062, 2156
MaeI	CTAG	5 cut(s)	287, 438, 879, 1064, 1608
MaeII	ACGT	6 cut(s)	145, 592, 716, 822, 1234, 2201
MaeIII	GTNAC	5 cut(s)	670, 1066, 1082, 1935, 2164
MfeI	CAATTG	2 cut(s)	891, 1275
MflI	RGATCY	5 cut(s)	20, 430, 874, 975, 1438
MhlI	GDGCHC	3 cut(s)	915, 1315, 1976
MluCI	AATT	8 cut(s)	61, 354, 855, 891, 1275, 1411, 1908, 2171
MluI	ACGCGT	1 cut(s)	1416
Mly113I	GGCGCC	1 cut(s)	159
MlyI	GAGTC	4 cut(s)	987, 1799, 1907, 2264
MmeI	TCCRAC	2 cut(s)	2184, 2238
MroXI	GAANNNNTTC	1 cut(s)	2127
MseI	TTAA	4 cut(s)	231, 353, 456, 1383
MspA1I	CMGCKG	2 cut(s)	695, 2078
MspI	CCGG	3 cut(s)	162, 752, 2030
MspR9I	CCNGG	2 cut(s)	512, 2037
MunI	CAATTG	2 cut(s)	891, 1275
Mva1269I	GAATGC	1 cut(s)	1467
MvaI	CCWGG	2 cut(s)	512, 2037
MvnI	CGCG	2 cut(s)	1418, 2354
MwoI	GCNNNNNNNGC	6 cut(s)	167, 1565, 1649, 1658, 2084, 2087
NarI	GGCGCC	1 cut(s)	159
NcoI	CCATGG	2 cut(s)	860, 1226
NheI	GCTAGC	1 cut(s)	286
NlaIV	GGNNCC	6 cut(s)	160, 516, 1074, 1440, 2034, 2310
NmuCI	GTSAC	1 cut(s)	1066
NspI	RCATGY	4 cut(s)	37, 678, 1096, 1714
PaeR7I	CTCGAG	3 cut(s)	1145, 1505, 1823
PagI	TCATGA	1 cut(s)	474
PciI	ACATGT	3 cut(s)	33, 674, 1092
PciSI	GCTCTTC	1 cut(s)	441
PctI	GAATGC	1 cut(s)	1467
PdmI	GAANNNNTTC	1 cut(s)	2127
PfeI	GAWTC	7 cut(s)	98, 211, 467, 896, 1246, 1293, 1361
PflFI	GACNNNGTC	1 cut(s)	824
PinAI	ACCGGT	1 cut(s)	751
PkrI	GCNGC	9 cut(s)	172, 1062, 1171, 1329, 1654, 1766, 2077, 2080, 2089
PleI	GAGTC	4 cut(s)	987, 1798, 1906, 2263
PluTI	GGCGCC	1 cut(s)	162
PpsI	GAGTC	4 cut(s)	987, 1798, 1906, 2263
PpuMI	RGGWCCY	2 cut(s)	514, 1948
PscI	ACATGT	3 cut(s)	33, 674, 1092
Psp124BI	GAGCTC	2 cut(s)	915, 1976
Psp5II	RGGWCCY	2 cut(s)	514, 1948
Psp6I	CCWGG	2 cut(s)	510, 2035
PspGI	CCWGG	2 cut(s)	510, 2035
PspN4I	GGNNCC	6 cut(s)	160, 516, 1074, 1440, 2034, 2310
PspPI	GGNCC	2 cut(s)	514, 1948
PspPPI	RGGWCCY	2 cut(s)	514, 1948
PspXI	VCTCGAGB	2 cut(s)	1145, 1505
PsrI	GAACNNNNNNTAC	4 cut(s)	173, 205, 732, 764
PstI	CTGCAG	1 cut(s)	2083
PstNI	CAGNNNCTG	1 cut(s)	101
PsuI	RGATCY	5 cut(s)	20, 430, 874, 975, 1438
PsyI	GACNNNGTC	1 cut(s)	824
PvuII	CAGCTG	2 cut(s)	695, 2078
SacI	GAGCTC	2 cut(s)	915, 1976
SapI	GCTCTTC	1 cut(s)	441
SaqAI	TTAA	4 cut(s)	231, 353, 456, 1383
SatI	GCNGC	9 cut(s)	171, 1061, 1170, 1328, 1653, 1765, 2076, 2079, 2088
Sau96I	GGNCC	2 cut(s)	514, 1948
SchI	GAGTC	4 cut(s)	987, 1799, 1907, 2264
ScrFI	CCNGG	2 cut(s)	512, 2037
SduI	GDGCHC	3 cut(s)	915, 1315, 1976
SfaNI	GCATC	8 cut(s)	157, 1478, 1587, 1605, 1700, 1894, 2062, 2156
SfcI	CTRYAG	1 cut(s)	2079
SfoI	GGCGCC	1 cut(s)	160
Sfr274I	CTCGAG	3 cut(s)	1145, 1505, 1823
SinI	GGWCC	2 cut(s)	514, 1948
SlaI	CTCGAG	3 cut(s)	1145, 1505, 1823
SmlI	CTYRAG	4 cut(s)	1145, 1505, 1823, 2336
SmoI	CTYRAG	4 cut(s)	1145, 1505, 1823, 2336
Sse9I	AATT	8 cut(s)	61, 354, 855, 891, 1275, 1411, 1908, 2171
SsiI	CCGC	7 cut(s)	67, 200, 342, 1442, 1652, 1892, 2354
SspDI	GGCGCC	1 cut(s)	158
SspI	AATATT	1 cut(s)	614
SspMI	CTAG	5 cut(s)	287, 438, 879, 1064, 1608
SstI	GAGCTC	2 cut(s)	915, 1976
StyD4I	CCNGG	2 cut(s)	510, 2035
StyI	CCWWGG	2 cut(s)	860, 1226
TaaI	ACNGT	9 cut(s)	108, 641, 661, 1007, 1072, 1672, 1868, 2304, 2330
TaiI	ACGT	6 cut(s)	148, 595, 719, 825, 1237, 2204
TasI	AATT	8 cut(s)	61, 354, 855, 891, 1275, 1411, 1908, 2171
TatI	WGTACW	4 cut(s)	180, 939, 1095, 2340
TauI	GCSGC	1 cut(s)	1655
TfiI	GAWTC	7 cut(s)	98, 211, 467, 896, 1246, 1293, 1361
Tru1I	TTAA	4 cut(s)	231, 353, 456, 1383
Tru9I	TTAA	4 cut(s)	231, 353, 456, 1383
TscAI	CASTG	3 cut(s)	835, 846, 1881
TseFI	GTSAC	1 cut(s)	1066
TseI	GCWGC	8 cut(s)	170, 1060, 1169, 1327, 1764, 2075, 2078, 2087
Tsp45I	GTSAC	1 cut(s)	1066
TspDTI	ATGAA	8 cut(s)	17, 381, 491, 810, 1369, 1650, 1905, 2257
TspGWI	ACGGA	2 cut(s)	156, 1725
TspRI	CASTG	3 cut(s)	835, 846, 1881
Tth111I	GACNNNGTC	1 cut(s)	824
VpaK11BI	GGWCC	2 cut(s)	514, 1948
XapI	RAATTY	3 cut(s)	61, 855, 2171
XceI	RCATGY	4 cut(s)	37, 678, 1096, 1714
XhoI	CTCGAG	3 cut(s)	1145, 1505, 1823
XmiI	GTMKAC	2 cut(s)	748, 1782
XmnI	GAANNNNTTC	1 cut(s)	2127
XspI	CTAG	5 cut(s)	287, 438, 879, 1064, 1608
ZraI	GACGTC	1 cut(s)	593

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.