Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0007461

Physical Location & Seq

Genomic Coordinates Reverse (-)

39494622 .. 39498800

Gene Structure 4179 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ32244

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2466 bp

ATGACGCGGAAGCAGAAGGCTGAGAACAAGGCACATGATGGAGAGCAACCTCCAAAGAAGCCTAGGGCTGATGATCACAATGGCCATGCAAATGGTAAAGCTTTACCTGCTGAGTTTGAGGAACTGTATTCTGCTGTCAAAGGGCATTTGTCAACAGAACAAATGCGAGAAATCTTGGAAGCTAATGATCAAGACTCTTCTGGCTCAGATGCTGCTGTTCTAAGAAAATGCCAAGATCTGCTATTCTATGGGCCATTAGATAGTTGTCGTATTTGCAATGGTAAATTAGAGTTCAATGGTATACGCTATTCTTGCACCGGAACTTATAGCGAGTGGGCTTCTTGTACTTTCAACACGAGGGATCCACCAAGGAAGCAGGAAGCGATCAAGTTACCTGAGAGTCTTGAGAAGTCTCCTGCTTCAGATTTGCTAAAGAAATATCAGGACCCAAGTCGCCGGCCCAAGAGGGATTTAAGCACTCCTGATAAGCCCTTCACTGGGATGTTAATTTCTTTAATGGGCCGTCTTACTAGATCACATGTATGTAGGACAGTTGATTTACAAACCGTCTTATTTGTCATTTCACGCTGTGTACTGATCATGCTTTCATTACCATTGCAGCAATACTGGAGACAGGAAGTAGATAAACATGGAGGAAAGGTTGCCAACCATGTGATTGGGACCTTTGCAGGTGTGACATGCCTAATTGTGTCTCCTGCGGAACGAGAACGCGGCGGCACAAATAAACTGGCTGAAGCAATGGAGAGAGGGATACCTGTAGTAAGTGAACAGTGGCTGATTGATAGCATAGAAAGACAAGAACCCCAGCCTCTCGAAGCATATGAAATTGCTAGTGATCTTGCTCCTGATGGCAGAGGAATCCCATGGGACAAAATGGATCCCAGTGAAGAGGCAATTGAGTCACTTTCAGCAGAACTCAAGCTTTATGGGAAGAGAGCTGTCTACAAAGATACGAAACTGCAGGAGCAAGGCGGAAAAATCTTCGAGAGGGATGGGACATTGTACAACTGTGCCTTCTCACATTGTGATCTAGGACGAAAACTGAACGATTATTGCATCTTGCAACTGATTATGGTGCCAGAGAACAGCCTGAACTTGTACTACAAGAAAGGCAGAGTTGGGGATGACCCCAATGCAGAGGAAAGGCTTGAAGAATGGAAGAATGTGGATGATGCTGTGAAAGAGTTTGTTAAAATATTTGAGGAAGTAACCGGAAATGAGTTTGAACCATGGGAAAGAGAGAAGAAGATCCAGAAGAAGCCATTTAAGTTTTACCCAATAGACATGGACGATGGAGTTGAAGTAAGACATGGAGGACTTGGTCTTCGACAGCTGGGAGTTGCTGCTACACATTGCAAACTTCAGCCCGAGGTTGCAAATTTTATGAAGATCCTATGCAGTCAGGAGGTTTACAAATATGCTCTGATGGAGATGGGATTGGATGCCCCAGAATTGCCAATGGGGATGCTTACCGATGTCCACCTAAAAAGATGTGAGGATGTTCTTCAAGAGTTTGTGGAGAAACTGAAGTCAATGAAGGAATCAGGGCCAGAGTCTGAGGCTGTTTGGTCGGACTTTAGCCAAAGATGGTTTACCCTGCTGCACTCTACTAGGCCTTTCATATTCAGGAACTTCCAGGAAGTTGCAGATCATGCTGCTGCTGCACTGGAGACTGTTCGGGACATAACTCTGTCCTCACATCTTATTGGAGACATGAGTGGAGCTACCATTGATGATCCCTTGTCAGATAGATGTAGCAAATTGGGATGCTCAATCTCCCCACTTGATAAGGAATCCGATGATTACAAAATGATTCTGAAGTACCTGGACAAGACCTATGAACCTGTCAAAGTTGGAGATATTGAATACGGGGTGTCTGTGGAGAACATATTCGTTGTTGAATCAAATGCCTGTCCATCTTTGGATGAAATAAAGAAGTTGCCTAACAAATTTCTCCTATGGTGTGGGACACGGAGCTCAAATCTGATGAGGCACTTGAATAAGGGTTTCTTGCCAGCAATATGCTCTCTCCCTGTCCCAGGCTATATGTTTGGGAAAGCAATTGTTTGCTCTGATACTGCCGCAGAGGCTGCAAGGTATGGTTATACTGCTGTGGACAGACCAGAGGGTTTCTTAGTTTTGGCTGTGGCTTCTCTAGGTGAAGAGATTATTGAGTTGAAAAACCCACCAGAGGACACAAAATCTTTAGAGGACAAGAAACTTGGAGTGAAGTGCATAGGGAAAAAGAAGACTGATGAATCGGAGCACTTTGTATGGCATGATTATATCAAAGTTCCCTGTGGACGATTAGTGCCTTCAGATAATAAAGACAGCATTGTCGAGTATAACGAGTATGCTGTCTATGATCCCAAGCAGACATGCATAAGGTTCTTGGTGGGTGTGAAGTATGAAGAGAAGGATGCTGTCATGGACACCGAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016874 GO:0016886 GO:0022616 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051103 GO:0051716 GO:0071704 GO:0090304 GO:0140097 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03410 ko04210 ko04212 ko04214 ko04217 map03410 map04210 map04212 map04214 map04217

Pfam Domains

Protein Families

BRCT BRCT_2 PADR1 PARP PARP_reg WGR

Protein Analysis

821

Amino Acids

92.71

Weight (kDa)

5.35

Isoelectric Point (pI)

44.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PADR1_N	PF21728	38 - 84	2.1e-12	PADR1, N-terminal helical domain
Zn_ribbon_PADR1	PF08063	86 - 125	8e-14	PADR1 domain, zinc ribbon fold
WGR	PF05406	342 - 420	1.1e-08	WGR domain
PARP_reg	PF02877	459 - 590	8.3e-24	Poly(ADP-ribose) polymerase, regulatory domain
PARP	PF00644	606 - 809	3.8e-50	Poly(ADP-ribose) polymerase catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22470
fragaria_vesca	FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670
malus_domestica	MD03G1142500.v1.1
prunus_persica	Prupe.6G127600_v2.0.a1 Prupe.6G127600_v2.0.a1 Prupe.6G127600_v2.0.a1 Prupe.6G127600_v2.0.a1
pyrus_communis	pycom03g09710
rosa_chinensis	RchiOBHm_Chr5g0044231
rosa_laevigata	RLG00000034257
rosa_multiflora	Rmu_sc0000776.1_g000071
rosa_roxburghii	Rroxscaffold_1G00036790
rosa_rugosa	Rorug05G0212700
rosa_samantha	Rh5AG296800 Rh5BG304300 Rh5CG331300 Rh5DG313100
rosa_wichuraiana	Rw5G027490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	680
AasI	GACNNNNNNGTC	1 cut(s)	2357
Acc36I	ACCTGC	2 cut(s)	115, 680
AccB1I	GGYRCC	1 cut(s)	1096
AccI	GTMKAC	2 cut(s)	301, 963
AccII	CGCG	2 cut(s)	7, 732
AciI	CCGC	6 cut(s)	7, 719, 732, 735, 993, 2103
AclWI	GGATC	8 cut(s)	356, 369, 893, 906, 1264, 1405, 1751, 2381
AcoI	YGGCCR	1 cut(s)	82
AcsI	RAATTY	2 cut(s)	1399, 1970
AcuI	CTGAAG	6 cut(s)	405, 774, 1367, 1568, 1859, 2322
AdeI	CACNNNGTG	2 cut(s)	590, 1046
AfaI	GTAC	5 cut(s)	346, 594, 1025, 1121, 1844
AfiI	CCNNNNNNNGG	4 cut(s)	497, 498, 2060, 2212
AflIII	ACRYGT	1 cut(s)	538
AjnI	CCWGG	3 cut(s)	1656, 1845, 2059
AluBI	AGCT	7 cut(s)	101, 182, 943, 959, 1354, 1745, 1998
AluI	AGCT	7 cut(s)	101, 182, 943, 959, 1354, 1745, 1998
Alw21I	GWGCWC	2 cut(s)	2000, 2289
Alw26I	GTCTC	5 cut(s)	417, 625, 717, 1685, 1725
AlwI	GGATC	8 cut(s)	356, 369, 893, 906, 1264, 1405, 1751, 2381
AlwNI	CAGNNNCTG	4 cut(s)	212, 796, 1577, 2111
Ama87I	CYCGRG	1 cut(s)	1388
AoxI	GGCC	6 cut(s)	82, 251, 458, 520, 1568, 1634
ApeKI	GCWGC	8 cut(s)	212, 619, 1364, 1621, 1676, 1679, 1682, 2111
ApoI	RAATTY	2 cut(s)	1399, 1970
ArsI	GACNNNNNNTTYG	2 cut(s)	1329, 1361
Asp700I	GAANNNNTTC	1 cut(s)	1910
AspA2I	CCTAGG	1 cut(s)	62
AspS9I	GGNCC	6 cut(s)	251, 445, 459, 520, 681, 1568
AsuHPI	GGTGA	1 cut(s)	2192
AvaI	CYCGRG	1 cut(s)	1388
AvaII	GGWCC	2 cut(s)	445, 681
AvrII	CCTAGG	1 cut(s)	62
BalI	TGGCCA	1 cut(s)	84
BamHI	GGATCC	2 cut(s)	361, 898
BanI	GGYRCC	1 cut(s)	1096
BanII	GRGCYC	1 cut(s)	2000
BauI	CACGAG	1 cut(s)	355
BbsI	GAAGAC	2 cut(s)	1337, 2276
Bbv12I	GWGCWC	2 cut(s)	2000, 2289
BbvI	GCAGC	8 cut(s)	199, 631, 1351, 1608, 1663, 1666, 1669, 2098
BccI	CCATC	8 cut(s)	32, 863, 1007, 1307, 1441, 1447, 1602, 1945
BceAI	ACGGC	1 cut(s)	507
BciT130I	CCWGG	3 cut(s)	1658, 1847, 2061
BciVI	GTATCC	1 cut(s)	765
BclI	TGATCA	3 cut(s)	73, 187, 597
BcoDI	GTCTC	5 cut(s)	417, 625, 717, 1685, 1725
BfaI	CTAG	6 cut(s)	63, 531, 852, 1052, 1632, 2177
BfmI	CTRYAG	2 cut(s)	777, 980
BfuAI	ACCTGC	2 cut(s)	115, 680
BfuI	GTATCC	1 cut(s)	765
BglI	GCCNNNNNGGC	1 cut(s)	2108
BglII	AGATCT	1 cut(s)	235
BlnI	CCTAGG	1 cut(s)	62
Bme1390I	CCNGG	3 cut(s)	1658, 1847, 2061
Bme18I	GGWCC	2 cut(s)	445, 681
BmeT110I	CYCGRG	1 cut(s)	1388
BmgT120I	GGNCC	6 cut(s)	251, 445, 459, 520, 681, 1568
BmiI	GGNNCC	5 cut(s)	363, 447, 682, 900, 1098
BmrFI	CCNGG	3 cut(s)	1658, 1847, 2061
BmrI	ACTGGG	2 cut(s)	507, 897
BmsI	GCATC	7 cut(s)	199, 1086, 1183, 1453, 1476, 1778, 2431
BmuI	ACTGGG	2 cut(s)	507, 897
BoxI	GACNNNNGTC	1 cut(s)	450
BpiI	GAAGAC	2 cut(s)	1337, 2276
BpmI	CTGGAG	2 cut(s)	649, 1709
BpuEI	CTTGAG	2 cut(s)	425, 923
BsaJI	CCNNGG	7 cut(s)	62, 368, 884, 1250, 1389, 2059, 2457
BsaWI	WCCGGW	2 cut(s)	317, 1232
BsaXI	ACNNNNNCTCC	4 cut(s)	645, 675, 1308, 1338
Bsc4I	CCNNNNNNNGG	4 cut(s)	497, 498, 2060, 2212
Bse118I	RCCGGY	1 cut(s)	456
Bse1I	ACTGG	5 cut(s)	502, 632, 753, 903, 1692
Bse3DI	GCAATG	4 cut(s)	283, 614, 765, 1372
BseBI	CCWGG	3 cut(s)	1658, 1847, 2061
BseDI	CCNNGG	7 cut(s)	62, 368, 884, 1250, 1389, 2059, 2457
BseLI	CCNNNNNNNGG	4 cut(s)	497, 498, 2060, 2212
BseMI	GCAATG	4 cut(s)	283, 614, 765, 1372
BseMII	CTCAG	5 cut(s)	12, 102, 219, 387, 1569
BseNI	ACTGG	5 cut(s)	502, 632, 753, 903, 1692
BseXI	GCAGC	8 cut(s)	199, 631, 1351, 1608, 1663, 1666, 1669, 2098
BseYI	CCCAGC	2 cut(s)	825, 1354
BsgI	GTGCAG	2 cut(s)	1607, 1668
Bsh1236I	CGCG	2 cut(s)	7, 732
BshFI	GGCC	6 cut(s)	84, 253, 460, 522, 1570, 1636
BshNI	GGYRCC	1 cut(s)	1096
BsiHKAI	GWGCWC	2 cut(s)	2000, 2289
BsiHKCI	CYCGRG	1 cut(s)	1388
BsiSI	CCGG	3 cut(s)	318, 457, 1233
BslFI	GGGAC	6 cut(s)	694, 902, 1030, 1715, 2002, 2042
BslI	CCNNNNNNNGG	4 cut(s)	497, 498, 2060, 2212
BsmAI	GTCTC	5 cut(s)	417, 625, 717, 1685, 1725
BsmFI	GGGAC	6 cut(s)	694, 902, 1030, 1715, 2002, 2042
BsnI	GGCC	6 cut(s)	84, 253, 460, 522, 1570, 1636
BsoBI	CYCGRG	1 cut(s)	1388
Bsp1286I	GDGCHC	2 cut(s)	2000, 2289
Bsp1407I	TGTACA	1 cut(s)	1023
Bsp19I	CCATGG	2 cut(s)	884, 1250
BspACI	CCGC	6 cut(s)	7, 719, 732, 735, 993, 2103
BspANI	GGCC	6 cut(s)	84, 253, 460, 522, 1570, 1636
BspCNI	CTCAG	5 cut(s)	13, 103, 218, 388, 1570
BspFNI	CGCG	2 cut(s)	7, 732
BspLI	GGNNCC	5 cut(s)	363, 447, 682, 900, 1098
BspMAI	CTGCAG	1 cut(s)	984
BspMI	ACCTGC	2 cut(s)	115, 680
BspPI	GGATC	8 cut(s)	356, 369, 893, 906, 1264, 1405, 1751, 2381
BspT107I	GGYRCC	1 cut(s)	1096
BsrDI	GCAATG	4 cut(s)	283, 614, 765, 1372
BsrFI	RCCGGY	1 cut(s)	456
BsrGI	TGTACA	1 cut(s)	1023
BsrI	ACTGG	5 cut(s)	502, 632, 753, 903, 1692
BssAI	RCCGGY	1 cut(s)	456
BssECI	CCNNGG	7 cut(s)	62, 368, 884, 1250, 1389, 2059, 2457
BssNAI	GTATAC	1 cut(s)	302
BssSI	CACGAG	1 cut(s)	355
BssT1I	CCWWGG	4 cut(s)	62, 368, 884, 1250
Bst1107I	GTATAC	1 cut(s)	302
Bst2BI	CACGAG	1 cut(s)	355
Bst2UI	CCWGG	3 cut(s)	1658, 1847, 2061
Bst4CI	ACNGT	6 cut(s)	126, 553, 568, 792, 1031, 1696
Bst6I	CTCTTC	5 cut(s)	202, 903, 947, 2178, 2427
BstAPI	GCANNNNNTGC	2 cut(s)	1673, 2111
BstAUI	TGTACA	1 cut(s)	1023
BstC8I	GCNNGC	2 cut(s)	458, 2037
BstDEI	CTNAG	7 cut(s)	21, 111, 205, 221, 396, 1578, 2155
BstDSI	CCRYGG	2 cut(s)	884, 1250
BstENI	CCTNNNNNAGG	1 cut(s)	2058
BstFNI	CGCG	2 cut(s)	7, 732
BstMAI	GTCTC	5 cut(s)	417, 625, 717, 1685, 1725
BstMWI	GCNNNNNNNGC	6 cut(s)	107, 312, 1673, 1682, 2108, 2111
BstNI	CCWGG	3 cut(s)	1658, 1847, 2061
BstNSI	RCATGY	3 cut(s)	542, 702, 2403
BstPAI	GACNNNNGTC	1 cut(s)	450
BstSCI	CCNGG	3 cut(s)	1656, 1845, 2059
BstSFI	CTRYAG	2 cut(s)	777, 980
BstUI	CGCG	2 cut(s)	7, 732
BstV1I	GCAGC	8 cut(s)	199, 631, 1351, 1608, 1663, 1666, 1669, 2098
BstV2I	GAAGAC	2 cut(s)	1337, 2276
BstX2I	RGATCY	5 cut(s)	235, 361, 898, 1269, 1410
BstXI	CCANNNNNNTGG	2 cut(s)	92, 677
BstYI	RGATCY	5 cut(s)	235, 361, 898, 1269, 1410
BstZ17I	GTATAC	1 cut(s)	302
BsuI	GTATCC	1 cut(s)	765
BsuRI	GGCC	6 cut(s)	84, 253, 460, 522, 1570, 1636
BtgI	CCRYGG	2 cut(s)	884, 1250
BtsIMutI	CAGTG	4 cut(s)	495, 797, 910, 1685
BveI	ACCTGC	2 cut(s)	115, 680
Cac8I	GCNNGC	2 cut(s)	458, 2037
CaiI	CAGNNNCTG	4 cut(s)	212, 796, 1577, 2111
Cfr10I	RCCGGY	1 cut(s)	456
Cfr13I	GGNCC	6 cut(s)	251, 445, 459, 520, 681, 1568
CseI	GACGC	1 cut(s)	13
Csp6I	GTAC	5 cut(s)	345, 593, 1024, 1120, 1843
CviQI	GTAC	5 cut(s)	345, 593, 1024, 1120, 1843
DdeI	CTNAG	7 cut(s)	21, 111, 205, 221, 396, 1578, 2155
DraIII	CACNNNGTG	2 cut(s)	590, 1046
DrdI	GACNNNNNNGTC	1 cut(s)	2357
DseDI	GACNNNNNNGTC	1 cut(s)	2357
EaeI	YGGCCR	1 cut(s)	82
Eam1104I	CTCTTC	5 cut(s)	202, 903, 947, 2178, 2427
EarI	CTCTTC	5 cut(s)	202, 903, 947, 2178, 2427
EciI	GGCGGA	1 cut(s)	1008
Ecl136II	GAGCTC	1 cut(s)	1998
Eco130I	CCWWGG	4 cut(s)	62, 368, 884, 1250
Eco147I	AGGCCT	1 cut(s)	1636
Eco24I	GRGCYC	1 cut(s)	2000
Eco47I	GGWCC	2 cut(s)	445, 681
Eco53kI	GAGCTC	1 cut(s)	1998
Eco57I	CTGAAG	6 cut(s)	405, 774, 1367, 1568, 1859, 2322
Eco88I	CYCGRG	1 cut(s)	1388
EcoICRI	GAGCTC	1 cut(s)	1998
EcoNI	CCTNNNNNAGG	1 cut(s)	2058
EcoO109I	RGGNCCY	2 cut(s)	445, 681
EcoRII	CCWGG	3 cut(s)	1656, 1845, 2059
EcoT14I	CCWWGG	4 cut(s)	62, 368, 884, 1250
EcoT22I	ATGCAT	1 cut(s)	2405
EcoT38I	GRGCYC	1 cut(s)	2000
ErhI	CCWWGG	4 cut(s)	62, 368, 884, 1250
FalI	AAGNNNNNCTT	2 cut(s)	2015, 2047
FaqI	GGGAC	6 cut(s)	694, 902, 1030, 1715, 2002, 2042
FauNDI	CATATG	1 cut(s)	841
FbaI	TGATCA	3 cut(s)	73, 187, 597
FblI	GTMKAC	2 cut(s)	301, 963
FriOI	GRGCYC	1 cut(s)	2000
FspBI	CTAG	6 cut(s)	63, 531, 852, 1052, 1632, 2177
GsaI	CCCAGC	2 cut(s)	829, 1358
GsuI	CTGGAG	2 cut(s)	649, 1709
HaeIII	GGCC	6 cut(s)	84, 253, 460, 522, 1570, 1636
HapII	CCGG	3 cut(s)	318, 457, 1233
HgaI	GACGC	1 cut(s)	13
HincII	GTYRAC	1 cut(s)	153
HindII	GTYRAC	1 cut(s)	153
HindIII	AAGCTT	2 cut(s)	99, 941
HpaII	CCGG	3 cut(s)	318, 457, 1233
HphI	GGTGA	1 cut(s)	2192
HpyAV	CCTTC	6 cut(s)	10, 502, 1045, 1552, 2346, 2431
HpyCH4III	ACNGT	6 cut(s)	126, 553, 568, 792, 1031, 1696
HpyF10VI	GCNNNNNNNGC	6 cut(s)	107, 312, 1673, 1682, 2108, 2111
HpyF3I	CTNAG	7 cut(s)	21, 111, 205, 221, 396, 1578, 2155
KroI	GCCGGC	1 cut(s)	456
KroNI	GCCGGC	1 cut(s)	458
Ksp22I	TGATCA	3 cut(s)	73, 187, 597
LmnI	GCTCC	5 cut(s)	868, 985, 1742, 1995, 2284
Lsp1109I	GCAGC	8 cut(s)	199, 631, 1351, 1608, 1663, 1666, 1669, 2098
LweI	GCATC	7 cut(s)	199, 1086, 1183, 1453, 1476, 1778, 2431
MaeI	CTAG	6 cut(s)	63, 531, 852, 1052, 1632, 2177
MaeIII	GTNAC	4 cut(s)	390, 694, 921, 1228
MfeI	CAATTG	2 cut(s)	915, 2082
MflI	RGATCY	5 cut(s)	235, 361, 898, 1269, 1410
MhlI	GDGCHC	2 cut(s)	2000, 2289
MlsI	TGGCCA	1 cut(s)	84
MluNI	TGGCCA	1 cut(s)	84
MlyI	GAGTC	4 cut(s)	188, 409, 929, 1583
MmeI	TCCRAC	2 cut(s)	1572, 1855
Mox20I	TGGCCA	1 cut(s)	84
Mph1103I	ATGCAT	1 cut(s)	2405
MroNI	GCCGGC	1 cut(s)	456
MroXI	GAANNNNTTC	1 cut(s)	1910
MscI	TGGCCA	1 cut(s)	84
MseI	TTAA	5 cut(s)	473, 506, 515, 1212, 1287
MslI	CAYNNNNRTG	3 cut(s)	90, 500, 541
Msp20I	TGGCCA	1 cut(s)	84
MspA1I	CMGCKG	1 cut(s)	1354
MspI	CCGG	3 cut(s)	318, 457, 1233
MspR9I	CCNGG	3 cut(s)	1658, 1847, 2061
MunI	CAATTG	2 cut(s)	915, 2082
MvaI	CCWGG	3 cut(s)	1658, 1847, 2061
MvnI	CGCG	2 cut(s)	7, 732
MwoI	GCNNNNNNNGC	6 cut(s)	107, 312, 1673, 1682, 2108, 2111
NaeI	GCCGGC	1 cut(s)	458
NcoI	CCATGG	2 cut(s)	884, 1250
NdeI	CATATG	1 cut(s)	841
NgoMIV	GCCGGC	1 cut(s)	456
NlaIV	GGNNCC	5 cut(s)	363, 447, 682, 900, 1098
NmuCI	GTSAC	2 cut(s)	694, 921
NsiI	ATGCAT	1 cut(s)	2405
NspI	RCATGY	3 cut(s)	542, 702, 2403
PaqCI	CACCTGC	1 cut(s)	680
PceI	AGGCCT	1 cut(s)	1636
PciI	ACATGT	1 cut(s)	538
PcsI	WCGNNNNNNNCGW	1 cut(s)	2367
PdiI	GCCGGC	1 cut(s)	458
PdmI	GAANNNNTTC	1 cut(s)	1910
PfeI	GAWTC	6 cut(s)	879, 1562, 1814, 1834, 1922, 2279
PflFI	GACNNNGTC	1 cut(s)	1341
PfoI	TCCNGGA	1 cut(s)	1656
PleI	GAGTC	4 cut(s)	188, 408, 928, 1582
PpsI	GAGTC	4 cut(s)	188, 408, 928, 1582
PpuMI	RGGWCCY	2 cut(s)	445, 681
PscI	ACATGT	1 cut(s)	538
PshAI	GACNNNNGTC	1 cut(s)	450
Psp124BI	GAGCTC	1 cut(s)	2000
Psp5II	RGGWCCY	2 cut(s)	445, 681
Psp6I	CCWGG	3 cut(s)	1656, 1845, 2059
PspFI	CCCAGC	2 cut(s)	825, 1354
PspGI	CCWGG	3 cut(s)	1656, 1845, 2059
PspN4I	GGNNCC	5 cut(s)	363, 447, 682, 900, 1098
PspPI	GGNCC	6 cut(s)	251, 445, 459, 520, 681, 1568
PspPPI	RGGWCCY	2 cut(s)	445, 681
PsrI	GAACNNNNNNTAC	2 cut(s)	1106, 1138
PstI	CTGCAG	1 cut(s)	984
PstNI	CAGNNNCTG	4 cut(s)	212, 796, 1577, 2111
PsuI	RGATCY	5 cut(s)	235, 361, 898, 1269, 1410
PsyI	GACNNNGTC	1 cut(s)	1341
PvuII	CAGCTG	1 cut(s)	1354
RsaI	GTAC	5 cut(s)	346, 594, 1025, 1121, 1844
RsaNI	GTAC	5 cut(s)	345, 593, 1024, 1120, 1843
RseI	CAYNNNNRTG	3 cut(s)	90, 500, 541
SacI	GAGCTC	1 cut(s)	2000
SaqAI	TTAA	5 cut(s)	473, 506, 515, 1212, 1287
Sau96I	GGNCC	6 cut(s)	251, 445, 459, 520, 681, 1568
SchI	GAGTC	4 cut(s)	188, 409, 929, 1583
ScrFI	CCNGG	3 cut(s)	1658, 1847, 2061
SduI	GDGCHC	2 cut(s)	2000, 2289
SfaNI	GCATC	7 cut(s)	199, 1086, 1183, 1453, 1476, 1778, 2431
SfcI	CTRYAG	2 cut(s)	777, 980
SinI	GGWCC	2 cut(s)	445, 681
SmiMI	CAYNNNNRTG	3 cut(s)	90, 500, 541
SmlI	CTYRAG	2 cut(s)	404, 938
SmoI	CTYRAG	2 cut(s)	404, 938
SseBI	AGGCCT	1 cut(s)	1636
SsiI	CCGC	6 cut(s)	7, 719, 732, 735, 993, 2103
SspI	AATATT	1 cut(s)	1218
SspMI	CTAG	6 cut(s)	63, 531, 852, 1052, 1632, 2177
SstI	GAGCTC	1 cut(s)	2000
StuI	AGGCCT	1 cut(s)	1636
StyD4I	CCNGG	3 cut(s)	1656, 1845, 2059
StyI	CCWWGG	4 cut(s)	62, 368, 884, 1250
TaaI	ACNGT	6 cut(s)	126, 553, 568, 792, 1031, 1696
TaqI	TCGA	4 cut(s)	834, 1005, 1348, 2361
TatI	WGTACW	4 cut(s)	344, 592, 1023, 1119
TauI	GCSGC	3 cut(s)	735, 738, 2105
TfiI	GAWTC	6 cut(s)	879, 1562, 1814, 1834, 1922, 2279
Tru1I	TTAA	5 cut(s)	473, 506, 515, 1212, 1287
Tru9I	TTAA	5 cut(s)	473, 506, 515, 1212, 1287
TscAI	CASTG	4 cut(s)	502, 797, 910, 1692
TseFI	GTSAC	2 cut(s)	694, 921
TseI	GCWGC	8 cut(s)	212, 619, 1364, 1621, 1676, 1679, 1682, 2111
Tsp45I	GTSAC	2 cut(s)	694, 921
TspDTI	ATGAA	9 cut(s)	597, 858, 1421, 1571, 1630, 1875, 1962, 2292, 2445
TspGWI	ACGGA	1 cut(s)	2008
TspRI	CASTG	4 cut(s)	502, 797, 910, 1692
Tth111I	GACNNNGTC	1 cut(s)	1341
VpaK11BI	GGWCC	2 cut(s)	445, 681
XagI	CCTNNNNNAGG	1 cut(s)	2058
XapI	RAATTY	2 cut(s)	1399, 1970
XceI	RCATGY	3 cut(s)	542, 702, 2403
XmaJI	CCTAGG	1 cut(s)	62
XmiI	GTMKAC	2 cut(s)	301, 963
XmnI	GAANNNNTTC	1 cut(s)	1910
XspI	CTAG	6 cut(s)	63, 531, 852, 1052, 1632, 2177
Zsp2I	ATGCAT	1 cut(s)	2405

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.