Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0007461

Physical Location & Seq

Genomic Coordinates Reverse (-)

40674913 .. 40679790

Gene Structure 4878 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G027490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2433 bp

ATGAAGGTTCATGAAAAGAGGTCACACTCTCATTCAAACCCTGAAGAGGAGAAGAAAGTAATGACGCGGAAGCAGAAGGCTGAGAACAAGGCACATGATGGAGAGCAACCTCCAAAGAAGCCTAGGGCTGATGATCACAATGGCCATGCAAATGGTAAAGCTTTACCTGCTGAGTTTGAGGAACTGTATTCTGCTGTCAAAGGGCATTTGTCAACTGAACAAATGCGAGAAATCTTGGAAGCTAATGATCAAGACTCTTCCGGCTCAGATGCTGCTGTTCTCAGAAAATGCCAAGATCTGCTATTCTATGGGCCATTAGATAGTTGTCGTATTTGCAATGGTAAATTAGAGTTCAATGGTATACGCTATTCTTGCACCGGAACTTATAGCGAGTGGGCTTCTTGTACTTTCAACACGAGGGATCCACCAAGGAAGCAGGAAGCGATCAAGTTACCTGAGAGTCTTGAGAAGTCTTCTGCTTCAGATTTGCTAAAGAACTATCAGGACCCAAGTCGCCGGCCCAAGAGGGATTTAAGCACTCCTGACAAGCCCTTCACTGGGATGTTAATTTCTCTAATGGGCCGTCTTACTAGATCACATCAATACTGGAGACAGGAAGTAGATAAACATGGAGGAAAGGTGGCCAACCATGTGATTGGTGTGACATGCCTAATTGTGTCTCCTGCGGAACGAGAACGAGGTGGCACAAATAAACTGGCTGAAGCAATGGAGAGAGGGATACCTGTAGTAAGTGAACAGTGGCTGATTGATAGCATAGAAAAACAAGAACCCCAGCCTCTCGAAGCATATGAAATTGCTAGTGATCTTGCTCCTGATGGCAGAGGAATCCCATGGGACAAAATGGATCCCAGTGAAGAGGCAATTGAGTCACTTTCAGCAGAACTCAAGCTTTATGGGAAGAGAGCTGTCTACAAAGATACGAAACTGAAGGAGCAAGGCGGAAAAATCTTCGAGAGGGATGGGACATTGTACAACTGTGCCTTCTCACATTGTGATCTAGGACGAAAACTGAACGATTATTGCATCTTGCAACTGATTATGGTGCCAGAGAACAGCCTGAACTTGTACTACAAGAAAGGCAGAGTTGGGGATGACCCCAATGCGGAGGAAAGGCTTGAAGAATGGAAGAATGTGGATGATGCTGTGAAAGAGTTTGTTAAAATATTTGAGGAAGTAACCGGAAATGAGTTTGAACCATGGGAAAGAGAGAAGAAGATCCAGAAGAAGCCATTTAAGTTTTACCCAATAGACATGGACGATGGAGTTGAAGTAAGACATGGAGGACTTGGTCTTCGACAGCTGGGAGTTGCTGCTACACATTGCAAACTTCAGCCCGAGGTTGCGAATTTTATGAAGATCTTATGCAGTCAGGAGATTTACAAATATGCTCTGATGGAGATGGGATTGGATGCCCCAGAATTGCCAGTGGGGATGCTTACAGATGTCCACCTAAAAAGATGTGAGGATGTTCTTCAAGAGTTTGTGGAGAAACTGAAGTCAATGAAGGAATCAGGGCCAGAGTCTGAGGCTGTTTGGTCGGACTTTAGCCAAAGATGGTTTACCCTGCTGCACTCTACTAGGCCTTTCATATTCAGGGACTTCCAGGAAGTTGCAGATCATGCTGCTGCTGCACTGGAGACTGTTCGGGACATAACTCTGTCCTCACATCTTATTGGAGACATGAGTGGAGCTACCATTGATGATCCCTTGTCCGATAGATGTAGCAAATTGGGATGCTCAATCTCCCCACTTGATAAGGAATCCGATGATTACAAAATGATTCTGAAGTACCTGGACAAGACCTATGAACCTGTCAAAGTTGGAGATATTGAATATGGGGTGTCTGTGGAGAACATATTCGTTGTTGAATCAAATGCCTGTCCATCTTTGGATGAAATAAAGAAGTTGCCTAACAAATTTCTCCTATGGTGTGGAACACGGAGCTCAAATCTGATGAGGCACTTGAATAAGGGTTTCTTGCCAGCAATATGCTCTCTCCCTGTCCCAGGCTATATGTTTGGGAAAGCAATTGTTTGCTCTGAAACTGCCGCGCAGGCTGCAAGGTATGGTTATACTGCTGTGGACAGACCAGAGGGTTTCTTAGTTTTGGCTGTGGCTTCTCTAGGTGAAGAGATTATTGAGTTGAAAAACCCACCAGAGGACACAAAATCTTTAGAGGACAAGAAAGTTGGAGTGAAGTGCATAGGGAAAAAGAAGACTGATGAATCGGAGCACTTTGTATGGCATGATGATATCAAAGTTCCTTGTGGACGATTAGTGCCTTCAGATAATAAAGACAGCATTGTCGAGTATAACGAGTATGCTGTCTATGATCCCAAGCAGACATGCATAAGGTTCTTGGTGGGTGTGAAGTATGAAGAGAAGGATGCTGTCATGGACACCGAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016874 GO:0016886 GO:0022616 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051103 GO:0051716 GO:0071704 GO:0090304 GO:0140097 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03410 ko04210 ko04212 ko04214 ko04217 map03410 map04210 map04212 map04214 map04217

Pfam Domains

Protein Families

BRCT BRCT_2 PADR1 PARP PARP_reg WGR

Protein Analysis

810

Amino Acids

91.57

Weight (kDa)

5.41

Isoelectric Point (pI)

44.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PADR1_N	PF21728	58 - 104	2e-12	PADR1, N-terminal helical domain
Zn_ribbon_PADR1	PF08063	106 - 145	7.9e-14	PADR1 domain, zinc ribbon fold
BRCT	PF00533	181 - 259	1.9e-07	BRCA1 C Terminus (BRCT) domain
BRCT_2	PF16589	185 - 271	1.5e-06	BRCT domain
WGR	PF05406	331 - 409	1.1e-08	WGR domain
PARP_reg	PF02877	448 - 579	2.5e-23	Poly(ADP-ribose) polymerase, regulatory domain
PARP	PF00644	595 - 798	7e-51	Poly(ADP-ribose) polymerase catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22470
fragaria_vesca	FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670 FvH4_3g24670
malus_domestica	MD03G1142500.v1.1
prunus_persica	Prupe.6G127600_v2.0.a1 Prupe.6G127600_v2.0.a1 Prupe.6G127600_v2.0.a1 Prupe.6G127600_v2.0.a1
pyrus_communis	pycom03g09710
rosa_chinensis	RchiOBHm_Chr5g0044231
rosa_laevigata	RLG00000034257
rosa_multiflora	Rmu_sc0000776.1_g000071
rosa_roxburghii	Rroxscaffold_1G00036790
rosa_rugosa	Rorug05G0212700
rosa_samantha	Rh5AG296800 Rh5BG304300 Rh5CG331300 Rh5DG313100
rosa_wichuraiana	Rw5G027490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2324
Acc36I	ACCTGC	1 cut(s)	175
AccB1I	GGYRCC	1 cut(s)	1063
AccI	GTMKAC	2 cut(s)	361, 930
AccII	CGCG	2 cut(s)	67, 2072
AciI	CCGC	5 cut(s)	67, 686, 960, 1124, 2070
AclWI	GGATC	7 cut(s)	416, 429, 860, 873, 1231, 1718, 2348
AcoI	YGGCCR	2 cut(s)	142, 642
AcsI	RAATTY	2 cut(s)	1366, 1937
AcuI	CTGAAG	8 cut(s)	63, 465, 741, 968, 1334, 1535, 1826, 2289
AdeI	CACNNNGTG	1 cut(s)	1013
AfaI	GTAC	4 cut(s)	406, 992, 1088, 1811
AfiI	CCNNNNNNNGG	6 cut(s)	46, 557, 558, 1123, 2027, 2179
AjnI	CCWGG	3 cut(s)	1623, 1812, 2026
AluBI	AGCT	7 cut(s)	161, 242, 910, 926, 1321, 1712, 1965
AluI	AGCT	7 cut(s)	161, 242, 910, 926, 1321, 1712, 1965
Alw21I	GWGCWC	2 cut(s)	1967, 2256
Alw26I	GTCTC	4 cut(s)	604, 684, 1652, 1692
AlwI	GGATC	7 cut(s)	416, 429, 860, 873, 1231, 1718, 2348
AlwNI	CAGNNNCTG	3 cut(s)	272, 763, 1544
Ama87I	CYCGRG	1 cut(s)	1355
AoxI	GGCC	7 cut(s)	142, 311, 518, 580, 642, 1535, 1601
ApeKI	GCWGC	7 cut(s)	272, 1331, 1588, 1643, 1646, 1649, 2078
ApoI	RAATTY	2 cut(s)	1366, 1937
ArsI	GACNNNNNNTTYG	2 cut(s)	1296, 1328
Asp700I	GAANNNNTTC	1 cut(s)	1877
AspA2I	CCTAGG	1 cut(s)	122
AspLEI	GCGC	1 cut(s)	2074
AspS9I	GGNCC	5 cut(s)	311, 505, 519, 580, 1535
AsuHPI	GGTGA	1 cut(s)	2159
AvaI	CYCGRG	1 cut(s)	1355
AvaII	GGWCC	1 cut(s)	505
AvrII	CCTAGG	1 cut(s)	122
BalI	TGGCCA	2 cut(s)	144, 644
BamHI	GGATCC	2 cut(s)	421, 865
BanI	GGYRCC	1 cut(s)	1063
BanII	GRGCYC	1 cut(s)	1967
BauI	CACGAG	1 cut(s)	415
BbsI	GAAGAC	3 cut(s)	465, 1304, 2243
Bbv12I	GWGCWC	2 cut(s)	1967, 2256
BbvI	GCAGC	7 cut(s)	259, 1318, 1575, 1630, 1633, 1636, 2065
BccI	CCATC	8 cut(s)	92, 830, 974, 1274, 1408, 1414, 1569, 1912
BceAI	ACGGC	1 cut(s)	567
BciT130I	CCWGG	3 cut(s)	1625, 1814, 2028
BciVI	GTATCC	1 cut(s)	732
BclI	TGATCA	2 cut(s)	133, 247
BcoDI	GTCTC	4 cut(s)	604, 684, 1652, 1692
BfaI	CTAG	6 cut(s)	123, 591, 819, 1019, 1599, 2144
BfmI	CTRYAG	1 cut(s)	744
BfuAI	ACCTGC	1 cut(s)	175
BfuI	GTATCC	1 cut(s)	732
BglI	GCCNNNNNGGC	1 cut(s)	2075
BglII	AGATCT	2 cut(s)	295, 1377
BisI	GCNGC	8 cut(s)	273, 1332, 1589, 1644, 1647, 1650, 2070, 2079
BlnI	CCTAGG	1 cut(s)	122
BlsI	GCNGC	8 cut(s)	274, 1333, 1590, 1645, 1648, 1651, 2071, 2080
Bme1390I	CCNGG	3 cut(s)	1625, 1814, 2028
Bme18I	GGWCC	1 cut(s)	505
BmeT110I	CYCGRG	1 cut(s)	1355
BmgT120I	GGNCC	5 cut(s)	311, 505, 519, 580, 1535
BmiI	GGNNCC	4 cut(s)	423, 507, 867, 1065
BmrFI	CCNGG	3 cut(s)	1625, 1814, 2028
BmrI	ACTGGG	2 cut(s)	567, 864
BmsI	GCATC	7 cut(s)	259, 1053, 1150, 1420, 1443, 1745, 2398
BmuI	ACTGGG	2 cut(s)	567, 864
BoxI	GACNNNNGTC	1 cut(s)	510
BpiI	GAAGAC	3 cut(s)	465, 1304, 2243
BpmI	CTGGAG	2 cut(s)	628, 1676
BpuEI	CTTGAG	2 cut(s)	485, 890
BsaJI	CCNNGG	7 cut(s)	122, 428, 851, 1217, 1356, 2026, 2424
BsaWI	WCCGGW	2 cut(s)	377, 1199
BsaXI	ACNNNNNCTCC	4 cut(s)	624, 654, 1275, 1305
Bsc4I	CCNNNNNNNGG	6 cut(s)	46, 557, 558, 1123, 2027, 2179
Bse118I	RCCGGY	1 cut(s)	516
Bse1I	ACTGG	6 cut(s)	562, 611, 720, 870, 1445, 1659
Bse3DI	GCAATG	3 cut(s)	343, 732, 1339
BseBI	CCWGG	3 cut(s)	1625, 1814, 2028
BseDI	CCNNGG	7 cut(s)	122, 428, 851, 1217, 1356, 2026, 2424
BseLI	CCNNNNNNNGG	6 cut(s)	46, 557, 558, 1123, 2027, 2179
BseMI	GCAATG	3 cut(s)	343, 732, 1339
BseMII	CTCAG	6 cut(s)	72, 162, 279, 295, 447, 1536
BseNI	ACTGG	6 cut(s)	562, 611, 720, 870, 1445, 1659
BseRI	GAGGAG	1 cut(s)	62
BseXI	GCAGC	7 cut(s)	259, 1318, 1575, 1630, 1633, 1636, 2065
BseYI	CCCAGC	2 cut(s)	792, 1321
BsgI	GTGCAG	2 cut(s)	1574, 1635
Bsh1236I	CGCG	2 cut(s)	67, 2072
BshFI	GGCC	7 cut(s)	144, 313, 520, 582, 644, 1537, 1603
BshNI	GGYRCC	1 cut(s)	1063
BsiHKAI	GWGCWC	2 cut(s)	1967, 2256
BsiHKCI	CYCGRG	1 cut(s)	1355
BsiSI	CCGG	4 cut(s)	261, 378, 517, 1200
BslFI	GGGAC	5 cut(s)	869, 997, 1631, 1682, 2009
BslI	CCNNNNNNNGG	6 cut(s)	46, 557, 558, 1123, 2027, 2179
BsmAI	GTCTC	4 cut(s)	604, 684, 1652, 1692
BsmFI	GGGAC	5 cut(s)	869, 997, 1631, 1682, 2009
BsnI	GGCC	7 cut(s)	144, 313, 520, 582, 644, 1537, 1603
BsoBI	CYCGRG	1 cut(s)	1355
Bsp1286I	GDGCHC	2 cut(s)	1967, 2256
Bsp1407I	TGTACA	1 cut(s)	990
Bsp19I	CCATGG	2 cut(s)	851, 1217
BspACI	CCGC	5 cut(s)	67, 686, 960, 1124, 2070
BspANI	GGCC	7 cut(s)	144, 313, 520, 582, 644, 1537, 1603
BspCNI	CTCAG	6 cut(s)	73, 163, 278, 294, 448, 1537
BspFNI	CGCG	2 cut(s)	67, 2072
BspHI	TCATGA	1 cut(s)	10
BspLI	GGNNCC	4 cut(s)	423, 507, 867, 1065
BspMI	ACCTGC	1 cut(s)	175
BspPI	GGATC	7 cut(s)	416, 429, 860, 873, 1231, 1718, 2348
BspT107I	GGYRCC	1 cut(s)	1063
BsrDI	GCAATG	3 cut(s)	343, 732, 1339
BsrFI	RCCGGY	1 cut(s)	516
BsrGI	TGTACA	1 cut(s)	990
BsrI	ACTGG	6 cut(s)	562, 611, 720, 870, 1445, 1659
BssAI	RCCGGY	1 cut(s)	516
BssECI	CCNNGG	7 cut(s)	122, 428, 851, 1217, 1356, 2026, 2424
BssNAI	GTATAC	1 cut(s)	362
BssSI	CACGAG	1 cut(s)	415
BssT1I	CCWWGG	4 cut(s)	122, 428, 851, 1217
Bst1107I	GTATAC	1 cut(s)	362
Bst2BI	CACGAG	1 cut(s)	415
Bst2UI	CCWGG	3 cut(s)	1625, 1814, 2028
Bst4CI	ACNGT	4 cut(s)	186, 759, 998, 1663
Bst6I	CTCTTC	6 cut(s)	39, 262, 870, 914, 2145, 2394
BstAPI	GCANNNNNTGC	1 cut(s)	1640
BstAUI	TGTACA	1 cut(s)	990
BstC8I	GCNNGC	3 cut(s)	518, 2004, 2076
BstDEI	CTNAG	7 cut(s)	81, 171, 265, 281, 456, 1545, 2122
BstDSI	CCRYGG	2 cut(s)	851, 1217
BstENI	CCTNNNNNAGG	1 cut(s)	2025
BstFNI	CGCG	2 cut(s)	67, 2072
BstHHI	GCGC	1 cut(s)	2074
BstMAI	GTCTC	4 cut(s)	604, 684, 1652, 1692
BstMWI	GCNNNNNNNGC	6 cut(s)	167, 372, 1640, 1649, 2075, 2078
BstNI	CCWGG	3 cut(s)	1625, 1814, 2028
BstNSI	RCATGY	2 cut(s)	669, 2370
BstPAI	GACNNNNGTC	1 cut(s)	510
BstSCI	CCNGG	3 cut(s)	1623, 1812, 2026
BstSFI	CTRYAG	1 cut(s)	744
BstUI	CGCG	2 cut(s)	67, 2072
BstV1I	GCAGC	7 cut(s)	259, 1318, 1575, 1630, 1633, 1636, 2065
BstV2I	GAAGAC	3 cut(s)	465, 1304, 2243
BstX2I	RGATCY	5 cut(s)	295, 421, 865, 1236, 1377
BstXI	CCANNNNNNTGG	2 cut(s)	152, 656
BstYI	RGATCY	5 cut(s)	295, 421, 865, 1236, 1377
BstZ17I	GTATAC	1 cut(s)	362
BsuI	GTATCC	1 cut(s)	732
BsuRI	GGCC	7 cut(s)	144, 313, 520, 582, 644, 1537, 1603
BtgI	CCRYGG	2 cut(s)	851, 1217
BtsIMutI	CAGTG	5 cut(s)	555, 764, 877, 1452, 1652
BveI	ACCTGC	1 cut(s)	175
Cac8I	GCNNGC	3 cut(s)	518, 2004, 2076
CaiI	CAGNNNCTG	3 cut(s)	272, 763, 1544
CciI	TCATGA	1 cut(s)	10
CfoI	GCGC	1 cut(s)	2074
Cfr10I	RCCGGY	1 cut(s)	516
Cfr13I	GGNCC	5 cut(s)	311, 505, 519, 580, 1535
CseI	GACGC	1 cut(s)	73
Csp6I	GTAC	4 cut(s)	405, 991, 1087, 1810
CviQI	GTAC	4 cut(s)	405, 991, 1087, 1810
DdeI	CTNAG	7 cut(s)	81, 171, 265, 281, 456, 1545, 2122
DraIII	CACNNNGTG	1 cut(s)	1013
DrdI	GACNNNNNNGTC	1 cut(s)	2324
DseDI	GACNNNNNNGTC	1 cut(s)	2324
EaeI	YGGCCR	2 cut(s)	142, 642
Eam1104I	CTCTTC	6 cut(s)	39, 262, 870, 914, 2145, 2394
EarI	CTCTTC	6 cut(s)	39, 262, 870, 914, 2145, 2394
EciI	GGCGGA	1 cut(s)	975
Ecl136II	GAGCTC	1 cut(s)	1965
Eco130I	CCWWGG	4 cut(s)	122, 428, 851, 1217
Eco147I	AGGCCT	1 cut(s)	1603
Eco24I	GRGCYC	1 cut(s)	1967
Eco32I	GATATC	1 cut(s)	2275
Eco47I	GGWCC	1 cut(s)	505
Eco53kI	GAGCTC	1 cut(s)	1965
Eco57I	CTGAAG	8 cut(s)	63, 465, 741, 968, 1334, 1535, 1826, 2289
Eco88I	CYCGRG	1 cut(s)	1355
EcoICRI	GAGCTC	1 cut(s)	1965
EcoNI	CCTNNNNNAGG	1 cut(s)	2025
EcoO109I	RGGNCCY	1 cut(s)	505
EcoRII	CCWGG	3 cut(s)	1623, 1812, 2026
EcoRV	GATATC	1 cut(s)	2275
EcoT14I	CCWWGG	4 cut(s)	122, 428, 851, 1217
EcoT22I	ATGCAT	1 cut(s)	2372
EcoT38I	GRGCYC	1 cut(s)	1967
ErhI	CCWWGG	4 cut(s)	122, 428, 851, 1217
FalI	AAGNNNNNCTT	2 cut(s)	1982, 2014
FaqI	GGGAC	5 cut(s)	869, 997, 1631, 1682, 2009
FauNDI	CATATG	1 cut(s)	808
FbaI	TGATCA	2 cut(s)	133, 247
FblI	GTMKAC	2 cut(s)	361, 930
Fnu4HI	GCNGC	8 cut(s)	273, 1332, 1589, 1644, 1647, 1650, 2070, 2079
FriOI	GRGCYC	1 cut(s)	1967
Fsp4HI	GCNGC	8 cut(s)	273, 1332, 1589, 1644, 1647, 1650, 2070, 2079
FspBI	CTAG	6 cut(s)	123, 591, 819, 1019, 1599, 2144
GlaI	GCGC	1 cut(s)	2073
GluI	GCNGC	8 cut(s)	273, 1332, 1589, 1644, 1647, 1650, 2070, 2079
GsaI	CCCAGC	2 cut(s)	796, 1325
GsuI	CTGGAG	2 cut(s)	628, 1676
HaeIII	GGCC	7 cut(s)	144, 313, 520, 582, 644, 1537, 1603
HapII	CCGG	4 cut(s)	261, 378, 517, 1200
HgaI	GACGC	1 cut(s)	73
HhaI	GCGC	1 cut(s)	2074
Hin6I	GCGC	1 cut(s)	2072
HinP1I	GCGC	1 cut(s)	2072
HincII	GTYRAC	1 cut(s)	213
HindII	GTYRAC	1 cut(s)	213
HindIII	AAGCTT	2 cut(s)	159, 908
HpaII	CCGG	4 cut(s)	261, 378, 517, 1200
HphI	GGTGA	1 cut(s)	2159
Hpy166II	GTNNAC	8 cut(s)	213, 362, 755, 931, 1468, 1581, 2104, 2291
Hpy8I	GTNNAC	8 cut(s)	213, 362, 755, 931, 1468, 1581, 2104, 2291
HpyAV	CCTTC	7 cut(s)	70, 562, 943, 1012, 1519, 2313, 2398
HpyCH4III	ACNGT	4 cut(s)	186, 759, 998, 1663
HpyF10VI	GCNNNNNNNGC	6 cut(s)	167, 372, 1640, 1649, 2075, 2078
HpyF3I	CTNAG	7 cut(s)	81, 171, 265, 281, 456, 1545, 2122
HspAI	GCGC	1 cut(s)	2072
KroI	GCCGGC	1 cut(s)	516
KroNI	GCCGGC	1 cut(s)	518
Ksp22I	TGATCA	2 cut(s)	133, 247
LmnI	GCTCC	5 cut(s)	835, 952, 1709, 1962, 2251
Lsp1109I	GCAGC	7 cut(s)	259, 1318, 1575, 1630, 1633, 1636, 2065
LweI	GCATC	7 cut(s)	259, 1053, 1150, 1420, 1443, 1745, 2398
MaeI	CTAG	6 cut(s)	123, 591, 819, 1019, 1599, 2144
MaeIII	GTNAC	5 cut(s)	21, 450, 661, 888, 1195
MfeI	CAATTG	2 cut(s)	882, 2049
MflI	RGATCY	5 cut(s)	295, 421, 865, 1236, 1377
MhlI	GDGCHC	2 cut(s)	1967, 2256
MlsI	TGGCCA	2 cut(s)	144, 644
MluNI	TGGCCA	2 cut(s)	144, 644
MlyI	GAGTC	4 cut(s)	248, 469, 896, 1550
MmeI	TCCRAC	3 cut(s)	1539, 1822, 2191
Mox20I	TGGCCA	2 cut(s)	144, 644
Mph1103I	ATGCAT	1 cut(s)	2372
MroNI	GCCGGC	1 cut(s)	516
MroXI	GAANNNNTTC	1 cut(s)	1877
MscI	TGGCCA	2 cut(s)	144, 644
MseI	TTAA	4 cut(s)	533, 566, 1179, 1254
MslI	CAYNNNNRTG	2 cut(s)	150, 560
Msp20I	TGGCCA	2 cut(s)	144, 644
MspA1I	CMGCKG	1 cut(s)	1321
MspI	CCGG	4 cut(s)	261, 378, 517, 1200
MspR9I	CCNGG	3 cut(s)	1625, 1814, 2028
MunI	CAATTG	2 cut(s)	882, 2049
MvaI	CCWGG	3 cut(s)	1625, 1814, 2028
MvnI	CGCG	2 cut(s)	67, 2072
MwoI	GCNNNNNNNGC	6 cut(s)	167, 372, 1640, 1649, 2075, 2078
NaeI	GCCGGC	1 cut(s)	518
NcoI	CCATGG	2 cut(s)	851, 1217
NdeI	CATATG	1 cut(s)	808
NgoMIV	GCCGGC	1 cut(s)	516
NlaIV	GGNNCC	4 cut(s)	423, 507, 867, 1065
NmuCI	GTSAC	3 cut(s)	21, 661, 888
NsiI	ATGCAT	1 cut(s)	2372
NspI	RCATGY	2 cut(s)	669, 2370
PagI	TCATGA	1 cut(s)	10
PceI	AGGCCT	1 cut(s)	1603
PcsI	WCGNNNNNNNCGW	1 cut(s)	2334
PdiI	GCCGGC	1 cut(s)	518
PdmI	GAANNNNTTC	1 cut(s)	1877
PfeI	GAWTC	6 cut(s)	846, 1529, 1781, 1801, 1889, 2246
PflFI	GACNNNGTC	1 cut(s)	1308
PfoI	TCCNGGA	1 cut(s)	1623
PkrI	GCNGC	8 cut(s)	274, 1333, 1590, 1645, 1648, 1651, 2071, 2080
PleI	GAGTC	4 cut(s)	248, 468, 895, 1549
PpsI	GAGTC	4 cut(s)	248, 468, 895, 1549
PpuMI	RGGWCCY	1 cut(s)	505
PshAI	GACNNNNGTC	1 cut(s)	510
Psp124BI	GAGCTC	1 cut(s)	1967
Psp5II	RGGWCCY	1 cut(s)	505
Psp6I	CCWGG	3 cut(s)	1623, 1812, 2026
PspFI	CCCAGC	2 cut(s)	792, 1321
PspGI	CCWGG	3 cut(s)	1623, 1812, 2026
PspN4I	GGNNCC	4 cut(s)	423, 507, 867, 1065
PspPI	GGNCC	5 cut(s)	311, 505, 519, 580, 1535
PspPPI	RGGWCCY	1 cut(s)	505
PsrI	GAACNNNNNNTAC	2 cut(s)	1073, 1105
PstNI	CAGNNNCTG	3 cut(s)	272, 763, 1544
PsuI	RGATCY	5 cut(s)	295, 421, 865, 1236, 1377
PsyI	GACNNNGTC	1 cut(s)	1308
PvuII	CAGCTG	1 cut(s)	1321
RsaI	GTAC	4 cut(s)	406, 992, 1088, 1811
RsaNI	GTAC	4 cut(s)	405, 991, 1087, 1810
RseI	CAYNNNNRTG	2 cut(s)	150, 560
SacI	GAGCTC	1 cut(s)	1967
SaqAI	TTAA	4 cut(s)	533, 566, 1179, 1254
SatI	GCNGC	8 cut(s)	273, 1332, 1589, 1644, 1647, 1650, 2070, 2079
Sau96I	GGNCC	5 cut(s)	311, 505, 519, 580, 1535
SchI	GAGTC	4 cut(s)	248, 469, 896, 1550
ScrFI	CCNGG	3 cut(s)	1625, 1814, 2028
SduI	GDGCHC	2 cut(s)	1967, 2256
SfaNI	GCATC	7 cut(s)	259, 1053, 1150, 1420, 1443, 1745, 2398
SfcI	CTRYAG	1 cut(s)	744
SinI	GGWCC	1 cut(s)	505
SmiMI	CAYNNNNRTG	2 cut(s)	150, 560
SmlI	CTYRAG	2 cut(s)	464, 905
SmoI	CTYRAG	2 cut(s)	464, 905
SseBI	AGGCCT	1 cut(s)	1603
SsiI	CCGC	5 cut(s)	67, 686, 960, 1124, 2070
SspI	AATATT	1 cut(s)	1185
SspMI	CTAG	6 cut(s)	123, 591, 819, 1019, 1599, 2144
SstI	GAGCTC	1 cut(s)	1967
StuI	AGGCCT	1 cut(s)	1603
StyD4I	CCNGG	3 cut(s)	1623, 1812, 2026
StyI	CCWWGG	4 cut(s)	122, 428, 851, 1217
TaaI	ACNGT	4 cut(s)	186, 759, 998, 1663
TaqI	TCGA	4 cut(s)	801, 972, 1315, 2328
TatI	WGTACW	3 cut(s)	404, 990, 1086
TauI	GCSGC	1 cut(s)	2072
TfiI	GAWTC	6 cut(s)	846, 1529, 1781, 1801, 1889, 2246
Tru1I	TTAA	4 cut(s)	533, 566, 1179, 1254
Tru9I	TTAA	4 cut(s)	533, 566, 1179, 1254
TscAI	CASTG	5 cut(s)	562, 764, 877, 1452, 1659
TseFI	GTSAC	3 cut(s)	21, 661, 888
TseI	GCWGC	7 cut(s)	272, 1331, 1588, 1643, 1646, 1649, 2078
Tsp45I	GTSAC	3 cut(s)	21, 661, 888
TspGWI	ACGGA	1 cut(s)	1975
TspRI	CASTG	5 cut(s)	562, 764, 877, 1452, 1659
Tth111I	GACNNNGTC	1 cut(s)	1308
VpaK11BI	GGWCC	1 cut(s)	505
XagI	CCTNNNNNAGG	1 cut(s)	2025
XapI	RAATTY	2 cut(s)	1366, 1937
XceI	RCATGY	2 cut(s)	669, 2370
XmaJI	CCTAGG	1 cut(s)	122
XmiI	GTMKAC	2 cut(s)	361, 930
XmnI	GAANNNNTTC	1 cut(s)	1877
XspI	CTAG	6 cut(s)	123, 591, 819, 1019, 1599, 2144
Zsp2I	ATGCAT	1 cut(s)	2372

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.