AT5G23480

Plus-3 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005324
Physical Location & Seq
Forward (+)
7915955 .. 7919328
3374 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G23480.6

Sequence Viewer

Length: 1257 bp
ATGGAGGATGATAATGAGAAAGTGAAAGGTTCGAGTAAAAAGAGGTTGAGAAAACCTAAAAGCTTGGAATTTGTTGGATGGGGTTCGAGAAATCTCATTGAGTTTCTTGAATCACTTGGTAGAGATACCACCAACAAGATATCAGAGAATGATGTTACAGCGATTATAATGAACTATATCCGCGAGAAGAGCCGTGAAACTCCTTTGAAGAGCAAGAAGAGGAGAAAGACAGTAGCTTGTGATGAGAAGTTGCGTTTGCTTTTCGGTGCTGGGAAGATAAATGTAATCAAAGTCCCTGATTTGGTAGAGAAGCATTATGTAGAGAATCAGGAGGAAGACTTATTCTATGATGATCTCTACGCTTCAGAGGATGATAAACAACAGAGATTGTCTCTATCAGATAAGGTGGCTAAACAGACGAAACAGGTTGTGTCAAAGCCTAGAGGGACTTTTGCTGCAATAGTGCGTGATACTGTTAAGCTTCTGTATTTGAGAAAGAGTTTAGTCCAGGAACTAGCAAAGACTCCTGAAACTTTTGAGAGCAAAGTGGTGAGGACTTTTGTGAGGATCAAGAATCCTTGTCAGCTTGTTCATGTAACCGGTGTGAAGGAAGGAGATCCGATTGATGGTAATCTTTTTCAGGTTACAAATTATTCGTATTATCTAAAAGATGTTACTACCTCTTCCCTATCAGATGATGATTTCTCTCAGGAGGAATGCGAAGAATTGCATCAGAGAATAAATAATGGCTTTGCTAAGAGACTTACAGTTGTTGATATGGAAGAAAAGGCTCGAAGTTTGCATGAAGATTATCTGGAGAAGAGGGAGCTTCTTCAGAATCCGGATGAACAGAAGAGGCTAGTGGATGAAGTTCCAGAAATTGTTGCAGAAGAACTTGAACCTGAATGTGAAGATGATGATGATGATAGGACAATAGAAGATAGCCTCATTGTACCAAACCCTGAGGCTCATCAAAGTGACAAAGAGCAACGACAAAGAGATTTACCAGTTTCTTCTAGTGTCAAGAAAAGTCAAGAAAATTCCTTGCTTCTTAAGAATCCGGAAGAGCAGTTGAGGCTGCTGTGTGATGTTCCCGATGTTGTTGCAGAAAAACTTGAACCTGAGTTTGTAGATGATGATGGAAAACTAGTAAATGACGTACCGTACCAAACCCTGAAGCCTTCACTGAGGCGCATCAAAGTGATGAAGAAATACAACCAAGTGATTTACCAGATTCTAGTATCCAGAAAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF Plus-3 SWIB

Protein Analysis

418

Amino Acids

48.38

Weight (kDa)

5.33

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIB PF02201 37 - 105 1.4e-07 SWIB/MDM2 domain
Plus-3 PF03126 159 - 247 3e-06 Plus-3 domain
NERD_plant PF25980 269 - 318 1.6e-23 Plant NERD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 167
AccII CGCG 1 cut(s) 183
AccIII TCCGGA 2 cut(s) 841, 1060
AciI CCGC 1 cut(s) 181
AclWI GGATC 2 cut(s) 575, 611
AcsI RAATTY 2 cut(s) 68, 1039
AcuI CTGAAG 3 cut(s) 348, 818, 1196
AfaI GTAC 3 cut(s) 954, 1161, 1166
AfiI CCNNNNNNNGG 2 cut(s) 301, 626
AflII CTTAAG 1 cut(s) 1052
AgeI ACCGGT 1 cut(s) 599
AgsI TTSAA 4 cut(s) 110, 208, 899, 1118
AhlI ACTAGT 1 cut(s) 1147
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 5 cut(s) 63, 236, 481, 586, 829
AluI AGCT 5 cut(s) 63, 236, 481, 586, 829
Alw26I GTCTC 2 cut(s) 396, 754
AlwI GGATC 2 cut(s) 575, 611
Aor13HI TCCGGA 2 cut(s) 841, 1060
ApeKI GCWGC 2 cut(s) 455, 1078
ApoI RAATTY 2 cut(s) 68, 1039
AsiGI ACCGGT 1 cut(s) 599
AspLEI GCGC 1 cut(s) 1194
AsuHPI GGTGA 1 cut(s) 562
AxyI CCTNAGG 1 cut(s) 963
BbsI GAAGAC 1 cut(s) 342
BbvI GCAGC 2 cut(s) 442, 1065
BccI CCATC 3 cut(s) 72, 620, 1133
BceAI ACGGC 1 cut(s) 177
BcgI CGANNNNNNTGC 2 cut(s) 1085, 1119
BciT130I CCWGG 1 cut(s) 509
BciVI GTATCC 1 cut(s) 1252
BcoDI GTCTC 2 cut(s) 396, 754
BcuI ACTAGT 1 cut(s) 1147
BfaI CTAG 7 cut(s) 441, 515, 860, 1017, 1148, 1238, 1255
BfrI CTTAAG 1 cut(s) 1052
BfuI GTATCC 1 cut(s) 1252
BisI GCNGC 2 cut(s) 456, 1079
BlsI GCNGC 2 cut(s) 457, 1080
Bme1390I CCNGG 1 cut(s) 509
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 2 cut(s) 739, 1203
BpiI GAAGAC 1 cut(s) 342
BplI GAGNNNNNCTC 2 cut(s) 376, 408
BpmI CTGGAG 1 cut(s) 836
BsaBI GATNNNNATC 1 cut(s) 630
BsaWI WCCGGW 3 cut(s) 599, 841, 1060
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 626
Bse118I RCCGGY 1 cut(s) 599
Bse1I ACTGG 1 cut(s) 1007
Bse21I CCTNAGG 1 cut(s) 963
Bse8I GATNNNNATC 1 cut(s) 630
BseAI TCCGGA 2 cut(s) 841, 1060
BseBI CCWGG 1 cut(s) 509
BseGI GGATG 5 cut(s) 13, 83, 376, 850, 871
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 2 cut(s) 301, 626
BseMII CTCAG 4 cut(s) 722, 954, 1113, 1178
BseNI ACTGG 1 cut(s) 1007
BseRI GAGGAG 1 cut(s) 235
BseXI GCAGC 2 cut(s) 442, 1065
BseYI CCCAGC 1 cut(s) 269
Bsh1236I CGCG 1 cut(s) 183
BshTI ACCGGT 1 cut(s) 599
BsiSI CCGG 3 cut(s) 600, 842, 1061
BslFI GGGAC 2 cut(s) 278, 460
BslI CCNNNNNNNGG 2 cut(s) 301, 626
BsmAI GTCTC 2 cut(s) 396, 754
BsmFI GGGAC 2 cut(s) 278, 460
BsmI GAATGC 1 cut(s) 722
Bsp13I TCCGGA 2 cut(s) 841, 1060
Bsp143I GATC 3 cut(s) 352, 567, 616
BspACI CCGC 1 cut(s) 181
BspCNI CTCAG 4 cut(s) 721, 955, 1114, 1179
BspEI TCCGGA 2 cut(s) 841, 1060
BspFNI CGCG 1 cut(s) 183
BspPI GGATC 2 cut(s) 575, 611
BspQI GCTCTTC 3 cut(s) 182, 203, 1059
BspTI CTTAAG 1 cut(s) 1052
BsrFI RCCGGY 1 cut(s) 599
BsrI ACTGG 1 cut(s) 1007
BssAI RCCGGY 1 cut(s) 599
BssMI GATC 3 cut(s) 352, 567, 616
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 4 cut(s) 232, 475, 769, 1164
Bst6I CTCTTC 7 cut(s) 182, 203, 212, 688, 815, 848, 1059
BstAFI CTTAAG 1 cut(s) 1052
BstDEI CTNAG 5 cut(s) 708, 756, 963, 1122, 1187
BstF5I GGATG 5 cut(s) 13, 83, 376, 850, 871
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 1 cut(s) 1194
BstKTI GATC 3 cut(s) 355, 570, 619
BstMAI GTCTC 2 cut(s) 396, 754
BstMBI GATC 3 cut(s) 352, 567, 616
BstMWI GCNNNNNNNGC 2 cut(s) 189, 1075
BstNI CCWGG 1 cut(s) 509
BstSCI CCNGG 1 cut(s) 507
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 442, 1065
BstV2I GAAGAC 1 cut(s) 342
BstX2I RGATCY 1 cut(s) 616
BstYI RGATCY 1 cut(s) 616
Bsu36I CCTNAGG 1 cut(s) 963
BsuI GTATCC 1 cut(s) 1252
BtsCI GGATG 5 cut(s) 13, 83, 376, 850, 871
BtsIMutI CAGTG 1 cut(s) 1184
CfoI GCGC 1 cut(s) 1194
Cfr10I RCCGGY 1 cut(s) 599
Csp6I GTAC 3 cut(s) 953, 1160, 1165
CspAI ACCGGT 1 cut(s) 599
CviAII CATG 2 cut(s) 593, 803
CviQI GTAC 3 cut(s) 953, 1160, 1165
DdeI CTNAG 5 cut(s) 708, 756, 963, 1122, 1187
DpnI GATC 3 cut(s) 354, 569, 618
DpnII GATC 3 cut(s) 352, 567, 616
Eam1104I CTCTTC 7 cut(s) 182, 203, 212, 688, 815, 848, 1059
EarI CTCTTC 7 cut(s) 182, 203, 212, 688, 815, 848, 1059
Eco32I GATATC 1 cut(s) 141
Eco57I CTGAAG 3 cut(s) 348, 818, 1196
Eco81I CCTNAGG 1 cut(s) 963
EcoRII CCWGG 1 cut(s) 507
EcoRV GATATC 1 cut(s) 141
FaeI CATG 2 cut(s) 596, 806
FaiI YATR 7 cut(s) 167, 177, 318, 348, 594, 779, 804
FaqI GGGAC 2 cut(s) 278, 460
FatI CATG 2 cut(s) 592, 802
Fnu4HI GCNGC 2 cut(s) 456, 1079
FokI GGATG 5 cut(s) 20, 90, 383, 857, 878
Fsp4HI GCNGC 2 cut(s) 456, 1079
FspBI CTAG 7 cut(s) 441, 515, 860, 1017, 1148, 1238, 1255
GlaI GCGC 1 cut(s) 1193
GluI GCNGC 2 cut(s) 456, 1079
GsaI CCCAGC 1 cut(s) 273
GsuI CTGGAG 1 cut(s) 836
HapII CCGG 3 cut(s) 600, 842, 1061
HhaI GCGC 1 cut(s) 1194
Hin1II CATG 2 cut(s) 596, 806
Hin6I GCGC 1 cut(s) 1192
HinP1I GCGC 1 cut(s) 1192
HindIII AAGCTT 2 cut(s) 61, 479
HinfI GANTC 7 cut(s) 110, 325, 523, 574, 838, 1057, 1234
HpaII CCGG 3 cut(s) 600, 842, 1061
HphI GGTGA 1 cut(s) 562
Hpy188I TCNGA 7 cut(s) 145, 367, 400, 621, 694, 735, 837
HpyAV CCTTC 3 cut(s) 601, 605, 1191
HpyCH4III ACNGT 4 cut(s) 232, 475, 769, 1164
HpyCH4IV ACGT 1 cut(s) 1158
HpyCH4V TGCA 5 cut(s) 458, 730, 802, 887, 1106
HpyF10VI GCNNNNNNNGC 2 cut(s) 189, 1075
HpyF3I CTNAG 5 cut(s) 708, 756, 963, 1122, 1187
HpySE526I ACGT 1 cut(s) 1158
Hsp92II CATG 2 cut(s) 596, 806
HspAI GCGC 1 cut(s) 1192
Kpn2I TCCGGA 2 cut(s) 841, 1060
Kzo9I GATC 3 cut(s) 352, 567, 616
LguI GCTCTTC 3 cut(s) 182, 203, 1059
LmnI GCTCC 1 cut(s) 826
Lsp1109I GCAGC 2 cut(s) 442, 1065
LweI GCATC 2 cut(s) 739, 1203
MaeI CTAG 7 cut(s) 441, 515, 860, 1017, 1148, 1238, 1255
MaeII ACGT 1 cut(s) 1158
MaeIII GTNAC 5 cut(s) 154, 595, 643, 673, 977
MalI GATC 3 cut(s) 354, 569, 618
MboI GATC 3 cut(s) 352, 567, 616
MflI RGATCY 1 cut(s) 616
MluCI AATT 5 cut(s) 68, 649, 725, 879, 1039
MlyI GAGTC 1 cut(s) 517
MmeI TCCRAC 1 cut(s) 55
MroI TCCGGA 2 cut(s) 841, 1060
MseI TTAA 2 cut(s) 477, 1053
MslI CAYNNNNRTG 2 cut(s) 975, 1199
MspCI CTTAAG 1 cut(s) 1052
MspI CCGG 3 cut(s) 600, 842, 1061
MspR9I CCNGG 1 cut(s) 509
Mva1269I GAATGC 1 cut(s) 722
MvaI CCWGG 1 cut(s) 509
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 2 cut(s) 189, 1075
NdeII GATC 3 cut(s) 352, 567, 616
NlaIII CATG 2 cut(s) 596, 806
NmuCI GTSAC 1 cut(s) 977
PciSI GCTCTTC 3 cut(s) 182, 203, 1059
PctI GAATGC 1 cut(s) 722
PfeI GAWTC 6 cut(s) 110, 325, 574, 838, 1057, 1234
PfoI TCCNGGA 1 cut(s) 507
PinAI ACCGGT 1 cut(s) 599
PkrI GCNGC 2 cut(s) 457, 1080
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
PsiI TTATAA 1 cut(s) 167
Psp6I CCWGG 1 cut(s) 507
PspFI CCCAGC 1 cut(s) 269
PspGI CCWGG 1 cut(s) 507
PsuI RGATCY 1 cut(s) 616
RsaI GTAC 3 cut(s) 954, 1161, 1166
RsaNI GTAC 3 cut(s) 953, 1160, 1165
RseI CAYNNNNRTG 2 cut(s) 975, 1199
SapI GCTCTTC 3 cut(s) 182, 203, 1059
SaqAI TTAA 2 cut(s) 477, 1053
SatI GCNGC 2 cut(s) 456, 1079
Sau3AI GATC 3 cut(s) 352, 567, 616
SchI GAGTC 1 cut(s) 517
ScrFI CCNGG 1 cut(s) 509
SfaNI GCATC 2 cut(s) 739, 1203
SmiMI CAYNNNNRTG 2 cut(s) 975, 1199
SmlI CTYRAG 1 cut(s) 1052
SmoI CTYRAG 1 cut(s) 1052
SpeI ACTAGT 1 cut(s) 1147
Sse9I AATT 5 cut(s) 68, 649, 725, 879, 1039
SsiI CCGC 1 cut(s) 181
SspMI CTAG 7 cut(s) 441, 515, 860, 1017, 1148, 1238, 1255
StyD4I CCNGG 1 cut(s) 507
TaaI ACNGT 4 cut(s) 232, 475, 769, 1164
TaiI ACGT 1 cut(s) 1161
TaqI TCGA 3 cut(s) 32, 86, 793
TasI AATT 5 cut(s) 68, 649, 725, 879, 1039
TfiI GAWTC 6 cut(s) 110, 325, 574, 838, 1057, 1234
Tru1I TTAA 2 cut(s) 477, 1053
Tru9I TTAA 2 cut(s) 477, 1053
TscAI CASTG 1 cut(s) 1191
TseFI GTSAC 1 cut(s) 977
TseI GCWGC 2 cut(s) 455, 1078
Tsp45I GTSAC 1 cut(s) 977
TspDTI ATGAA 6 cut(s) 185, 581, 819, 861, 882, 1220
TspRI CASTG 1 cut(s) 1191
Vha464I CTTAAG 1 cut(s) 1052
XapI RAATTY 2 cut(s) 68, 1039
XspI CTAG 7 cut(s) 441, 515, 860, 1017, 1148, 1238, 1255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.