Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0005324

Physical Location & Seq

Genomic Coordinates Forward (+)

19630829 .. 19635709

Gene Structure 4881 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023186

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1647 bp

ATGGGCTCTTTCACTTGGGTTGGGGAGTACAACGCCGCCGTCACAGCCACCGCCGATGTCGACGCCGCCGCCGCCGCTGCTCCGACGAGGAGGAGCAAGAGGCTTCACCGCCCCAAGAAGTTTGAGTTTCTGAGCTGGGGCTCGAAGCCGCTGATTGAATTTCTCGAGTCGATTGGGAAGGACACGACGAAGCAGATCTCCCAATACGACGTCGCTACGATTATCACCGACTACGTCAACCACCGCAGGCTTCTCCACCCGACGAAGAGGAAGAGGATCGTCTGCGACGAGGGGCTTCATTCTCTGTTCGGCAGGAAGACCATTGCTCGGATCAAGATCTACGATTTGCTCCAGCCGCATTTCGCTGAGAATCTCGAGGACGATTCCGATGACTCGTCGGATGAGTCCGACGACGACGGTTTGGACGAGGAGGACGAGGATAAGCAGCGGCGGCAGCAGCAGCAGAGGAAGAAGCAGCAGAAGAGGAGAGCCGCTGCCGCTCTGCATGCGCCTCCTCCGGTTCCGAAGAGCTGCTACGCCGCCGTGATTCCGGAGAATTTGAAGCTGGTGTACCTGAGGAGGTCGGTGGTGGAAGCTCTGCTGAAGCAGGACGAGGCTCAGGTGTTTGAGGACAAAGTCGTGGGGAGCTTTGTGAGGACGAAATCGGACCCCAATGACTATCTGCAGAAGAATTCTCACCAGCTAATGCAGGTGAAGGCGATTCTGAAGAAGCCGGAGCCGAATTCGGAATCGTCTGCTGGAGGTGGAGTTCTTCTTCAGCTTCATGGGGCTGTTAAAGACTTCCCCATTTCCATGCTCTCTGATGATAACTTCTTCCCGGAAGAATGTGAGGATTTACGTGAAAGAGTTAAAGGTGGTTTGCAGAAGAGACCTACAGTGGTGGAGCTTCAACAAAAGGTCCAGATTTTGCATCTGGATGTAACAAAGCATTGGCTTGTGAGAGAACTTCAATACTTACAGAACTCAATTGATCGAGCGAATGAAAAGGGATTGCGCCGAGAATATCCTTTTAAATTATTTACAGCTTATATGCCATACTTGAATATAAGGGAACTGCTGCAGTCTCCAGATGAACAAGCACGTCTTTTGCGAGAGGTTCCCGAAGTTATTGCAGAAGAATTAGAGGAAGAAACAAAAGATTCTCCAGATGAAACAGAACAAGGAAACCATGGCTCCCCAGGACATATCCTCAGGGGAGAATCAACTCCTATTTCTGAAGCATGCGAGGATAATGATTCTTTGCAGAACTGGCAGAAGCAACCTGCAGAATCTGTAAAAGAGATACTTGATGCAGAGGACAACAAGGCTTCTCAGCAACTTGTGGATAAGCATGTAGTGACATGTGAGGTGGTGGATGTAAGTGATGACGACGAAAATGAAGAACCAGAATCTGAAGAAAAGTACGAGATTCCTATTCCCCAGCTGGGAACCTTAATGTGGCATTATTCCGATCCCCAGGGAAACATACAGGGTCCTTTCTCAATCATTTCCTTAAAGCGCTGGAGTGATTCTGACTACTTCCCTCCAGATTTCAAAATTTGGAAAACAGGTCAGAGTAGTAATGAAGCTGTATTATTGACCAAAATTCTTCGTGGGAATTTTCTAAATAAAAGTGTATATCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GYF Plus-3 SWIB

Protein Analysis

549

Amino Acids

62.57

Weight (kDa)

5.19

Isoelectric Point (pI)

65.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SWIB	PF02201	48 - 121	1.7e-09	SWIB/MDM2 domain
Plus-3	PF03126	186 - 290	5.3e-14	Plus-3 domain
NERD_plant	PF25980	313 - 369	1.9e-19	Plant NERD domain
GYF	PF02213	487 - 527	2.5e-12	GYF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005324)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08430 AT5G08430 AT5G23480 AT5G23480 AT5G23480 AT5G23480 AT5G23480 AT5G23480
fragaria_vesca	FvH4_1g25810 FvH4_1g25810 FvH4_1g25810
malus_domestica	MD15G1140400.v1.1
prunus_persica	Prupe.4G286800_v2.0.a1 Prupe.4G286800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0484911 RchiOBHm_Chr3g0484921
rosa_laevigata	RLG00000013602 RLG00000023186
rosa_multiflora	Rmu_sc0002231.1_g000024 Rmu_sc0005946.1_g000003 Rmu_sc0005946.1_g000004
rosa_roxburghii	Rroxscaffold_6G00397360
rosa_rugosa	Rorug03G0214200
rosa_samantha	Rh3AG264600 Rh3BG300100 Rh3CG297800 Rh3DG293900 Rh6CG195000
rosa_wichuraiana	Rw3G023650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	700
AatII	GACGTC	1 cut(s)	213
Acc36I	ACCTGC	2 cut(s)	700, 1291
AccBSI	CCGCTC	1 cut(s)	500
AccI	GTMKAC	1 cut(s)	60
AccIII	TCCGGA	1 cut(s)	550
AclWI	GGATC	3 cut(s)	284, 338, 1466
AcsI	RAATTY	7 cut(s)	158, 556, 691, 742, 1557, 1605, 1618
AcuI	CTGAAG	5 cut(s)	623, 746, 761, 1257, 1434
AcyI	GRCGYC	2 cut(s)	63, 210
AfaI	GTAC	3 cut(s)	29, 572, 1424
AfeI	AGCGCT	1 cut(s)	1520
AfiI	CCNNNNNNNGG	3 cut(s)	327, 1445, 1458
AflIII	ACRYGT	1 cut(s)	1361
AgsI	TTSAA	6 cut(s)	158, 562, 911, 971, 1063, 1555
AjiI	CACGTC	1 cut(s)	1103
AjnI	CCWGG	2 cut(s)	1198, 1476
AloI	GAACNNNNNNTCC	2 cut(s)	753, 785
Alw26I	GTCTC	2 cut(s)	883, 1089
AlwI	GGATC	3 cut(s)	284, 338, 1466
AlwNI	CAGNNNCTG	2 cut(s)	1292, 1412
Ama87I	CYCGRG	2 cut(s)	164, 374
Aor13HI	TCCGGA	1 cut(s)	550
Aor51HI	AGCGCT	1 cut(s)	1520
ApeKI	GCWGC	9 cut(s)	77, 445, 454, 457, 460, 475, 494, 531, 1078
ApoI	RAATTY	7 cut(s)	158, 556, 691, 742, 1557, 1605, 1618
AspLEI	GCGC	3 cut(s)	511, 1017, 1521
AspS9I	GGNCC	3 cut(s)	667, 919, 1493
AsuC2I	CCSGG	1 cut(s)	839
AsuHPI	GGTGA	4 cut(s)	98, 217, 689, 724
AvaI	CYCGRG	2 cut(s)	164, 374
AvaII	GGWCC	3 cut(s)	667, 919, 1493
AxyI	CCTNAGG	2 cut(s)	575, 1211
BanII	GRGCYC	2 cut(s)	8, 143
BarI	GAAGNNNNNNTAC	4 cut(s)	518, 550, 554, 586
BbsI	GAAGAC	1 cut(s)	323
BbvI	GCAGC	9 cut(s)	64, 457, 466, 469, 472, 481, 487, 518, 1065
BceAI	ACGGC	2 cut(s)	23, 527
BcgI	CGANNNNNNTGC	2 cut(s)	1112, 1146
BciT130I	CCWGG	2 cut(s)	1200, 1478
BcnI	CCSGG	1 cut(s)	839
BcoDI	GTCTC	2 cut(s)	883, 1089
BfmI	CTRYAG	4 cut(s)	683, 894, 1079, 1284
BfoI	RGCGCY	1 cut(s)	1522
BfuAI	ACCTGC	2 cut(s)	700, 1291
BglII	AGATCT	2 cut(s)	195, 336
Bme1390I	CCNGG	3 cut(s)	839, 1200, 1478
Bme18I	GGWCC	3 cut(s)	667, 919, 1493
BmeT110I	CYCGRG	2 cut(s)	164, 374
BmgBI	CACGTC	1 cut(s)	1103
BmgT120I	GGNCC	3 cut(s)	667, 919, 1493
BmiI	GGNNCC	7 cut(s)	522, 669, 738, 1119, 1195, 1450, 1494
BmrFI	CCNGG	3 cut(s)	839, 1200, 1478
BmsI	GCATC	2 cut(s)	940, 1300
BpiI	GAAGAC	1 cut(s)	323
BpmI	CTGGAG	6 cut(s)	335, 780, 1071, 1149, 1530, 1543
Bpu10I	CCTNAGC	1 cut(s)	618
BpuMI	CCSGG	1 cut(s)	839
BsaAI	YACGTR	1 cut(s)	860
BsaBI	GATNNNNATC	1 cut(s)	335
BsaHI	GRCGYC	2 cut(s)	63, 210
BsaI	GGTCTC	1 cut(s)	883
BsaJI	CCNNGG	4 cut(s)	1189, 1198, 1476, 1477
BsaWI	WCCGGW	2 cut(s)	517, 550
BsaXI	ACNNNNNCTCC	4 cut(s)	753, 783, 1178, 1208
Bsc4I	CCNNNNNNNGG	3 cut(s)	327, 1445, 1458
Bse1I	ACTGG	1 cut(s)	1274
Bse21I	CCTNAGG	2 cut(s)	575, 1211
Bse3DI	GCAATG	1 cut(s)	321
Bse8I	GATNNNNATC	1 cut(s)	335
BseAI	TCCGGA	1 cut(s)	550
BseBI	CCWGG	2 cut(s)	1200, 1478
BseDI	CCNNGG	4 cut(s)	1189, 1198, 1476, 1477
BseGI	GGATG	3 cut(s)	406, 943, 1381
BseJI	GATNNNNATC	1 cut(s)	335
BseLI	CCNNNNNNNGG	3 cut(s)	327, 1445, 1458
BseMI	GCAATG	1 cut(s)	321
BseMII	CTCAG	6 cut(s)	122, 357, 566, 632, 1225, 1346
BseNI	ACTGG	1 cut(s)	1274
BseRI	GAGGAG	6 cut(s)	103, 106, 443, 499, 504, 592
BseXI	GCAGC	9 cut(s)	64, 457, 466, 469, 472, 481, 487, 518, 1065
BseYI	CCCAGC	3 cut(s)	135, 1440, 1444
BsiHKCI	CYCGRG	2 cut(s)	164, 374
BsiSI	CCGG	4 cut(s)	518, 551, 734, 839
BslI	CCNNNNNNNGG	3 cut(s)	327, 1445, 1458
BsmAI	GTCTC	2 cut(s)	883, 1089
Bso31I	GGTCTC	1 cut(s)	883
BsoBI	CYCGRG	2 cut(s)	164, 374
Bsp1286I	GDGCHC	2 cut(s)	8, 143
Bsp13I	TCCGGA	1 cut(s)	550
Bsp143I	GATC	6 cut(s)	195, 276, 330, 336, 991, 1471
Bsp19I	CCATGG	1 cut(s)	1189
BspCNI	CTCAG	6 cut(s)	123, 358, 567, 631, 1224, 1345
BspEI	TCCGGA	1 cut(s)	550
BspLI	GGNNCC	7 cut(s)	522, 669, 738, 1119, 1195, 1450, 1494
BspMAI	CTGCAG	3 cut(s)	687, 1083, 1288
BspMI	ACCTGC	2 cut(s)	700, 1291
BspPI	GGATC	3 cut(s)	284, 338, 1466
BspQI	GCTCTTC	1 cut(s)	521
BspTNI	GGTCTC	1 cut(s)	883
BsrBI	CCGCTC	1 cut(s)	500
BsrDI	GCAATG	1 cut(s)	321
BsrI	ACTGG	1 cut(s)	1274
BssECI	CCNNGG	4 cut(s)	1189, 1198, 1476, 1477
BssMI	GATC	6 cut(s)	195, 276, 330, 336, 991, 1471
BssNI	GRCGYC	2 cut(s)	63, 210
BssT1I	CCWWGG	1 cut(s)	1189
Bst2UI	CCWGG	2 cut(s)	1200, 1478
Bst4CI	ACNGT	2 cut(s)	419, 898
Bst6I	CTCTTC	5 cut(s)	260, 266, 476, 521, 881
BstACI	GRCGYC	2 cut(s)	63, 210
BstBAI	YACGTR	1 cut(s)	860
BstC8I	GCNNGC	3 cut(s)	248, 507, 1243
BstDEI	CTNAG	6 cut(s)	131, 366, 575, 618, 1211, 1332
BstDSI	CCRYGG	1 cut(s)	1189
BstF5I	GGATG	3 cut(s)	406, 943, 1381
BstH2I	RGCGCY	1 cut(s)	1522
BstHHI	GCGC	3 cut(s)	511, 1017, 1521
BstKTI	GATC	6 cut(s)	198, 279, 333, 339, 994, 1474
BstMAI	GTCTC	2 cut(s)	883, 1089
BstMBI	GATC	6 cut(s)	195, 276, 330, 336, 991, 1471
BstNI	CCWGG	2 cut(s)	1200, 1478
BstNSI	RCATGY	4 cut(s)	509, 1245, 1355, 1365
BstSCI	CCNGG	3 cut(s)	837, 1198, 1476
BstSFI	CTRYAG	4 cut(s)	683, 894, 1079, 1284
BstV1I	GCAGC	9 cut(s)	64, 457, 466, 469, 472, 481, 487, 518, 1065
BstV2I	GAAGAC	1 cut(s)	323
BstX2I	RGATCY	2 cut(s)	195, 336
BstYI	RGATCY	2 cut(s)	195, 336
Bsu36I	CCTNAGG	2 cut(s)	575, 1211
BtgI	CCRYGG	1 cut(s)	1189
BtrI	CACGTC	1 cut(s)	1103
BtsCI	GGATG	3 cut(s)	406, 943, 1381
BtsIMutI	CAGTG	1 cut(s)	903
BveI	ACCTGC	2 cut(s)	700, 1291
Cac8I	GCNNGC	3 cut(s)	248, 507, 1243
CaiI	CAGNNNCTG	2 cut(s)	1292, 1412
CfoI	GCGC	3 cut(s)	511, 1017, 1521
Cfr13I	GGNCC	3 cut(s)	667, 919, 1493
CseI	GACGC	1 cut(s)	71
Csp6I	GTAC	3 cut(s)	28, 571, 1423
CviAII	CATG	7 cut(s)	506, 785, 814, 1190, 1242, 1352, 1362
CviQI	GTAC	3 cut(s)	28, 571, 1423
DdeI	CTNAG	6 cut(s)	131, 366, 575, 618, 1211, 1332
DpnI	GATC	6 cut(s)	197, 278, 332, 338, 993, 1473
DpnII	GATC	6 cut(s)	195, 276, 330, 336, 991, 1471
DraI	TTTAAA	1 cut(s)	1033
Eam1104I	CTCTTC	5 cut(s)	260, 266, 476, 521, 881
EarI	CTCTTC	5 cut(s)	260, 266, 476, 521, 881
Eco130I	CCWWGG	1 cut(s)	1189
Eco24I	GRGCYC	2 cut(s)	8, 143
Eco31I	GGTCTC	1 cut(s)	883
Eco47I	GGWCC	3 cut(s)	667, 919, 1493
Eco47III	AGCGCT	1 cut(s)	1520
Eco57I	CTGAAG	5 cut(s)	623, 746, 761, 1257, 1434
Eco81I	CCTNAGG	2 cut(s)	575, 1211
Eco88I	CYCGRG	2 cut(s)	164, 374
EcoO109I	RGGNCCY	1 cut(s)	1493
EcoRI	GAATTC	2 cut(s)	691, 742
EcoRII	CCWGG	2 cut(s)	1198, 1476
EcoT14I	CCWWGG	1 cut(s)	1189
EcoT38I	GRGCYC	2 cut(s)	8, 143
ErhI	CCWWGG	1 cut(s)	1189
FaeI	CATG	7 cut(s)	509, 788, 817, 1193, 1245, 1355, 1365
FalI	AAGNNNNNCTT	4 cut(s)	1089, 1121, 1290, 1322
FatI	CATG	7 cut(s)	505, 784, 813, 1189, 1241, 1351, 1361
FblI	GTMKAC	1 cut(s)	60
FokI	GGATG	3 cut(s)	413, 950, 1388
FriOI	GRGCYC	2 cut(s)	8, 143
GlaI	GCGC	3 cut(s)	510, 1016, 1520
GsaI	CCCAGC	3 cut(s)	139, 1444, 1448
GsuI	CTGGAG	6 cut(s)	335, 780, 1071, 1149, 1530, 1543
HaeII	RGCGCY	1 cut(s)	1522
HapII	CCGG	4 cut(s)	518, 551, 734, 839
HgaI	GACGC	1 cut(s)	71
HhaI	GCGC	3 cut(s)	511, 1017, 1521
Hin1I	GRCGYC	2 cut(s)	63, 210
Hin1II	CATG	7 cut(s)	509, 788, 817, 1193, 1245, 1355, 1365
Hin6I	GCGC	3 cut(s)	509, 1015, 1519
HinP1I	GCGC	3 cut(s)	509, 1015, 1519
HincII	GTYRAC	2 cut(s)	61, 238
HindII	GTYRAC	2 cut(s)	61, 238
HpaII	CCGG	4 cut(s)	518, 551, 734, 839
HphI	GGTGA	4 cut(s)	98, 217, 689, 724
Hpy166II	GTNNAC	3 cut(s)	61, 238, 571
Hpy8I	GTNNAC	3 cut(s)	61, 238, 571
HpyAV	CCTTC	2 cut(s)	172, 709
HpyCH4III	ACNGT	2 cut(s)	419, 898
HpyCH4IV	ACGT	4 cut(s)	210, 234, 859, 1102
HpyF3I	CTNAG	6 cut(s)	131, 366, 575, 618, 1211, 1332
HpySE526I	ACGT	4 cut(s)	210, 234, 859, 1102
Hsp92I	GRCGYC	2 cut(s)	63, 210
Hsp92II	CATG	7 cut(s)	509, 788, 817, 1193, 1245, 1355, 1365
HspAI	GCGC	3 cut(s)	509, 1015, 1519
Kpn2I	TCCGGA	1 cut(s)	550
Kzo9I	GATC	6 cut(s)	195, 276, 330, 336, 991, 1471
LguI	GCTCTTC	1 cut(s)	521
LmnI	GCTCC	7 cut(s)	85, 93, 354, 645, 736, 904, 1199
Lsp1109I	GCAGC	9 cut(s)	64, 457, 466, 469, 472, 481, 487, 518, 1065
LweI	GCATC	2 cut(s)	940, 1300
MaeII	ACGT	4 cut(s)	210, 234, 859, 1102
MaeIII	GTNAC	3 cut(s)	40, 940, 1357
MalI	GATC	6 cut(s)	197, 278, 332, 338, 993, 1473
MbiI	CCGCTC	1 cut(s)	500
MboI	GATC	6 cut(s)	195, 276, 330, 336, 991, 1471
MfeI	CAATTG	1 cut(s)	987
MflI	RGATCY	2 cut(s)	195, 336
MhlI	GDGCHC	2 cut(s)	8, 143
MlyI	GAGTC	3 cut(s)	176, 386, 413
MmeI	TCCRAC	3 cut(s)	107, 378, 432
MroI	TCCGGA	1 cut(s)	550
MseI	TTAA	5 cut(s)	795, 870, 1032, 1454, 1514
MslI	CAYNNNNRTG	2 cut(s)	812, 936
MspA1I	CMGCKG	5 cut(s)	77, 151, 448, 494, 1444
MspI	CCGG	4 cut(s)	518, 551, 734, 839
MspR9I	CCNGG	3 cut(s)	839, 1200, 1478
MunI	CAATTG	1 cut(s)	987
MvaI	CCWGG	2 cut(s)	1200, 1478
NciI	CCSGG	1 cut(s)	839
NcoI	CCATGG	1 cut(s)	1189
NdeII	GATC	6 cut(s)	195, 276, 330, 336, 991, 1471
NlaIII	CATG	7 cut(s)	509, 788, 817, 1193, 1245, 1355, 1365
NlaIV	GGNNCC	7 cut(s)	522, 669, 738, 1119, 1195, 1450, 1494
NmeAIII	GCCGAG	1 cut(s)	1043
NmuCI	GTSAC	2 cut(s)	40, 1357
NspI	RCATGY	4 cut(s)	509, 1245, 1355, 1365
PaeI	GCATGC	2 cut(s)	509, 1245
PaeR7I	CTCGAG	2 cut(s)	164, 374
PaqCI	CACCTGC	1 cut(s)	700
PasI	CCCWGGG	1 cut(s)	1477
PciI	ACATGT	1 cut(s)	1361
PciSI	GCTCTTC	1 cut(s)	521
PcsI	WCGNNNNNNNCGW	4 cut(s)	285, 423, 432, 1108
PflFI	GACNNNGTC	2 cut(s)	233, 635
PfoI	TCCNGGA	1 cut(s)	837
PleI	GAGTC	3 cut(s)	175, 386, 412
PpsI	GAGTC	3 cut(s)	175, 386, 412
Ppu21I	YACGTR	1 cut(s)	860
PpuMI	RGGWCCY	1 cut(s)	1493
PscI	ACATGT	1 cut(s)	1361
Psp5II	RGGWCCY	1 cut(s)	1493
Psp6I	CCWGG	2 cut(s)	1198, 1476
PspFI	CCCAGC	3 cut(s)	135, 1440, 1444
PspGI	CCWGG	2 cut(s)	1198, 1476
PspN4I	GGNNCC	7 cut(s)	522, 669, 738, 1119, 1195, 1450, 1494
PspPI	GGNCC	3 cut(s)	667, 919, 1493
PspPPI	RGGWCCY	1 cut(s)	1493
PstI	CTGCAG	3 cut(s)	687, 1083, 1288
PstNI	CAGNNNCTG	2 cut(s)	1292, 1412
PsuI	RGATCY	2 cut(s)	195, 336
PsyI	GACNNNGTC	2 cut(s)	233, 635
PvuII	CAGCTG	1 cut(s)	1444
RsaI	GTAC	3 cut(s)	29, 572, 1424
RsaNI	GTAC	3 cut(s)	28, 571, 1423
RseI	CAYNNNNRTG	2 cut(s)	812, 936
SalI	GTCGAC	1 cut(s)	59
SapI	GCTCTTC	1 cut(s)	521
SaqAI	TTAA	5 cut(s)	795, 870, 1032, 1454, 1514
Sau3AI	GATC	6 cut(s)	195, 276, 330, 336, 991, 1471
Sau96I	GGNCC	3 cut(s)	667, 919, 1493
SchI	GAGTC	3 cut(s)	176, 386, 413
ScrFI	CCNGG	3 cut(s)	839, 1200, 1478
SduI	GDGCHC	2 cut(s)	8, 143
SfaNI	GCATC	2 cut(s)	940, 1300
SfcI	CTRYAG	4 cut(s)	683, 894, 1079, 1284
Sfr274I	CTCGAG	2 cut(s)	164, 374
SinI	GGWCC	3 cut(s)	667, 919, 1493
SlaI	CTCGAG	2 cut(s)	164, 374
SmiMI	CAYNNNNRTG	2 cut(s)	812, 936
SmlI	CTYRAG	2 cut(s)	164, 374
SmoI	CTYRAG	2 cut(s)	164, 374
SphI	GCATGC	2 cut(s)	509, 1245
StyD4I	CCNGG	3 cut(s)	837, 1198, 1476
StyI	CCWWGG	1 cut(s)	1189
TaaI	ACNGT	2 cut(s)	419, 898
TaiI	ACGT	4 cut(s)	213, 237, 862, 1105
TaqI	TCGA	6 cut(s)	60, 143, 165, 170, 375, 994
TatI	WGTACW	1 cut(s)	27
Tru1I	TTAA	5 cut(s)	795, 870, 1032, 1454, 1514
Tru9I	TTAA	5 cut(s)	795, 870, 1032, 1454, 1514
TscAI	CASTG	1 cut(s)	903
TseFI	GTSAC	2 cut(s)	40, 1357
TseI	GCWGC	9 cut(s)	77, 445, 454, 457, 460, 475, 494, 531, 1078
Tsp45I	GTSAC	2 cut(s)	40, 1357
TspDTI	ATGAA	7 cut(s)	287, 773, 1017, 1107, 1185, 1413, 1599
TspRI	CASTG	1 cut(s)	903
Tth111I	GACNNNGTC	2 cut(s)	233, 635
VpaK11BI	GGWCC	3 cut(s)	667, 919, 1493
XapI	RAATTY	7 cut(s)	158, 556, 691, 742, 1557, 1605, 1618
XceI	RCATGY	4 cut(s)	509, 1245, 1355, 1365
XhoI	CTCGAG	2 cut(s)	164, 374
XmiI	GTMKAC	1 cut(s)	60
ZraI	GACGTC	1 cut(s)	211

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.