Rorug03G0214200

At5g08430

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005324
Physical Location & Seq
Reverse (-)
19352404 .. 19353044
641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0214200.1

Sequence Viewer

Length: 555 bp
ATGAGTTCTTTTAAGTATTTGGTGAAAGTTGACAAGTTTCCTGGTCCCGAGAAAGTAAAGCATTTTGTTGATTTTATCCGATGCTACTACTTGCCCAAGTGTGACAGCAAGAGTGACGATGAGATTATCAATGACGTTCCTAATGTAACAGCAATGGACGAGGCTGGAGTTCAGTTTGAGGGACGGAAGGAATTGACCACATCGCTTGACATAGATGATTGGACTGAGACTCTCTTCAGGAATCTCATCGCGTTTGAGCAATGCCATGATCACAAAGTAAAGTACATCACACAGTTTATGTTCCTCATGGGGTGTATGATCAGGACTCCAAAGGATGCAGATTATTTACTCGTCAATCGCGAAATTGTATCTTGCAGGCTAGGGAATAGTGAAGATATTTCAACTATATTCAAGAGCCCATGGCACAGTTGGAAAGCAACTCTGAAGAGGGACTATTTCAACACTCCATGGAAACTGGTTTCTACAATTGCAGCATTTGTATTGCTTGTCCTCACTGTTATACAAACAGTGTTCTCCATCATATCCGTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF Plus-3 SWIB

Protein Analysis

184

Amino Acids

21.34

Weight (kDa)

6.08

Isoelectric Point (pI)

26.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 15 - 138 5.8e-23 Plant protein of unknown function
DUF247 PF03140 131 - 171 2.4e-08 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 251, 360
AcuI CTGAAG 2 cut(s) 220, 464
AfaI GTAC 1 cut(s) 284
AgsI TTSAA 3 cut(s) 402, 412, 460
AjnI CCWGG 1 cut(s) 40
Alw26I GTCTC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 47
ApeKI GCWGC 1 cut(s) 491
AspS9I GGNCC 1 cut(s) 44
AsuHPI GGTGA 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 419
BbvI GCAGC 1 cut(s) 503
BccI CCATC 1 cut(s) 545
BciT130I CCWGG 1 cut(s) 42
BclI TGATCA 2 cut(s) 268, 318
BcoDI GTCTC 1 cut(s) 221
BfaI CTAG 1 cut(s) 380
BisI GCNGC 1 cut(s) 492
BlsI GCNGC 1 cut(s) 493
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 44
BmeT110I CYCGRG 1 cut(s) 47
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 1 cut(s) 46
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 2 cut(s) 71, 325
BpmI CTGGAG 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 419, 467
Bse1I ACTGG 1 cut(s) 480
Bse3DI GCAATG 2 cut(s) 159, 266
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 2 cut(s) 419, 467
BseGI GGATG 1 cut(s) 340
BseMI GCAATG 2 cut(s) 159, 266
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 480
BseXI GCAGC 1 cut(s) 503
Bsh1236I CGCG 2 cut(s) 251, 360
BsiHKCI CYCGRG 1 cut(s) 47
BslFI GGGAC 3 cut(s) 30, 195, 464
BsmAI GTCTC 1 cut(s) 221
BsmFI GGGAC 3 cut(s) 30, 195, 464
BsoBI CYCGRG 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 419
Bsp143I GATC 2 cut(s) 268, 318
Bsp19I CCATGG 2 cut(s) 419, 467
Bsp68I TCGCGA 1 cut(s) 360
BspCNI CTCAG 1 cut(s) 217
BspFNI CGCG 2 cut(s) 251, 360
BspLI GGNNCC 1 cut(s) 46
BsrDI GCAATG 2 cut(s) 159, 266
BsrI ACTGG 1 cut(s) 480
BssECI CCNNGG 2 cut(s) 419, 467
BssMI GATC 2 cut(s) 268, 318
BssT1I CCWWGG 2 cut(s) 419, 467
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 4 cut(s) 294, 428, 517, 529
Bst6I CTCTTC 2 cut(s) 239, 440
BstC8I GCNNGC 1 cut(s) 377
BstDEI CTNAG 1 cut(s) 225
BstDSI CCRYGG 2 cut(s) 419, 467
BstF5I GGATG 1 cut(s) 340
BstFNI CGCG 2 cut(s) 251, 360
BstKTI GATC 2 cut(s) 271, 321
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 2 cut(s) 268, 318
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 40
BstUI CGCG 2 cut(s) 251, 360
BstV1I GCAGC 1 cut(s) 503
BtgI CCRYGG 2 cut(s) 419, 467
BtgZI GCGATG 2 cut(s) 186, 232
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 2 cut(s) 513, 534
BtuMI TCGCGA 1 cut(s) 360
Cac8I GCNNGC 1 cut(s) 377
Cfr13I GGNCC 1 cut(s) 44
Csp6I GTAC 1 cut(s) 283
CspCI CAANNNNNGTGG 2 cut(s) 187, 222
CviAII CATG 4 cut(s) 266, 307, 420, 468
CviJI RGCY 3 cut(s) 164, 379, 417
CviKI_1 RGCY 3 cut(s) 164, 379, 417
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 1 cut(s) 225
DpnI GATC 2 cut(s) 270, 320
DpnII GATC 2 cut(s) 268, 318
Eam1104I CTCTTC 2 cut(s) 239, 440
EarI CTCTTC 2 cut(s) 239, 440
Eco130I CCWWGG 2 cut(s) 419, 467
Eco24I GRGCYC 1 cut(s) 419
Eco47I GGWCC 1 cut(s) 44
Eco57I CTGAAG 2 cut(s) 220, 464
Eco88I CYCGRG 1 cut(s) 47
EcoRII CCWGG 1 cut(s) 40
EcoT14I CCWWGG 2 cut(s) 419, 467
EcoT38I GRGCYC 1 cut(s) 419
ErhI CCWWGG 2 cut(s) 419, 467
FaeI CATG 4 cut(s) 269, 310, 423, 471
FaqI GGGAC 3 cut(s) 30, 195, 464
FatI CATG 4 cut(s) 265, 306, 419, 467
FbaI TGATCA 2 cut(s) 268, 318
Fnu4HI GCNGC 1 cut(s) 492
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 1 cut(s) 419
Fsp4HI GCNGC 1 cut(s) 492
FspBI CTAG 1 cut(s) 380
GluI GCNGC 1 cut(s) 492
GsuI CTGGAG 1 cut(s) 186
Hin1II CATG 4 cut(s) 269, 310, 423, 471
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 3 cut(s) 229, 241, 325
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 2 cut(s) 80, 444
Hpy188III TCNNGA 5 cut(s) 47, 238, 322, 359, 412
Hpy8I GTNNAC 1 cut(s) 31
HpyAV CCTTC 1 cut(s) 181
HpyCH4III ACNGT 4 cut(s) 294, 428, 517, 529
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 3 cut(s) 338, 375, 491
HpyF3I CTNAG 1 cut(s) 225
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 4 cut(s) 269, 310, 423, 471
Ksp22I TGATCA 2 cut(s) 268, 318
Kzo9I GATC 2 cut(s) 268, 318
LpnPI CCDG 7 cut(s) 27, 54, 150, 223, 307, 361, 461
Lsp1109I GCAGC 1 cut(s) 503
LweI GCATC 2 cut(s) 71, 325
MaeI CTAG 1 cut(s) 380
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 3 cut(s) 101, 113, 145
MalI GATC 2 cut(s) 270, 320
MboI GATC 2 cut(s) 268, 318
MboII GAAGA 3 cut(s) 226, 404, 457
MfeI CAATTG 1 cut(s) 486
MhlI GDGCHC 1 cut(s) 419
MluCI AATT 3 cut(s) 191, 363, 486
MlyI GAGTC 2 cut(s) 223, 319
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 5 cut(s) 154, 172, 314, 441, 521
MseI TTAA 2 cut(s) 12, 553
MspR9I CCNGG 1 cut(s) 42
MunI CAATTG 1 cut(s) 486
MvaI CCWGG 1 cut(s) 42
MvnI CGCG 2 cut(s) 251, 360
NcoI CCATGG 2 cut(s) 419, 467
NdeII GATC 2 cut(s) 268, 318
NlaIII CATG 4 cut(s) 269, 310, 423, 471
NlaIV GGNNCC 1 cut(s) 46
NmuCI GTSAC 2 cut(s) 101, 113
NruI TCGCGA 1 cut(s) 360
PcsI WCGNNNNNNNCGW 1 cut(s) 357
PfeI GAWTC 1 cut(s) 241
PkrI GCNGC 1 cut(s) 493
PleI GAGTC 2 cut(s) 223, 319
PpsI GAGTC 2 cut(s) 223, 319
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 1 cut(s) 46
PspPI GGNCC 1 cut(s) 44
RruI TCGCGA 1 cut(s) 360
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
SaqAI TTAA 2 cut(s) 12, 553
SatI GCNGC 1 cut(s) 492
Sau3AI GATC 2 cut(s) 268, 318
Sau96I GGNCC 1 cut(s) 44
SchI GAGTC 2 cut(s) 223, 319
ScrFI CCNGG 1 cut(s) 42
SduI GDGCHC 1 cut(s) 419
SetI ASST 1 cut(s) 138
SfaNI GCATC 2 cut(s) 71, 325
SinI GGWCC 1 cut(s) 44
Sse9I AATT 3 cut(s) 191, 363, 486
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 2 cut(s) 419, 467
TaaI ACNGT 4 cut(s) 294, 428, 517, 529
TaiI ACGT 1 cut(s) 138
TasI AATT 3 cut(s) 191, 363, 486
TatI WGTACW 1 cut(s) 282
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 2 cut(s) 12, 553
Tru9I TTAA 2 cut(s) 12, 553
TscAI CASTG 2 cut(s) 520, 534
TseFI GTSAC 2 cut(s) 101, 113
TseI GCWGC 1 cut(s) 491
Tsp45I GTSAC 2 cut(s) 101, 113
TspGWI ACGGA 2 cut(s) 199, 535
TspRI CASTG 2 cut(s) 520, 534
VpaK11BI GGWCC 1 cut(s) 44
XcmI CCANNNNNNNNNTGG 1 cut(s) 426
XspI CTAG 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.