AT5G23550

May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012878
Physical Location & Seq
Reverse (-)
7939597 .. 7941677
2081 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G23550.1

Sequence Viewer

Length: 528 bp
ATGGATAAAATGAATCAGGCTATTGAGAAGATGAAGATGTTGGTTGGTATGGAGGTTGAAGACGAGCAACAAGCTGCTGATGAAGAAAGCTCGCTCTCATTCATGGAAGATCTTAATCGCAATTGCGCTTTAACTACCAAACAGAGATTTTATGGCTTTGCAATTTGCTTGTCAGCAGGTTTGACCTGTACACTTTTGTCGATGCTTGTTTTCTTTAATCCGGTCAAGTTTGGCATCACATTCACTCTGGGAAATTTGATGGCACTCGGGAGCACAGCATTCCTTATAGGCCCACAGCGGCAGGTGACTATGATGCTTGACCCAGCTCGTATCTACGCTACTGCATTGTATCTAGCAAGCATTATCATTGCCTTGTTTTGTGCTCTTTATGTTCGCAACAAGCTTCTGACACTGCTGGCTATCATTCTTGAGTTCACTGGTCTAATTTGGTATAGCTTGAGCTACATCCCTTTTGCAAGAACCATGGTCTCAAAGATCTTCATGACTTGTTTTGACACCGAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0006810 GO:0008150 GO:0016020 GO:0016192 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Got1

Protein Analysis

175

Amino Acids

19.74

Weight (kDa)

5.14

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Got1 PF04178 51 - 157 3e-30 Got1/Sft2-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 292
Acc36I ACCTGC 2 cut(s) 167, 292
AciI CCGC 1 cut(s) 298
AcsI RAATTY 1 cut(s) 253
AfaI GTAC 1 cut(s) 190
AgsI TTSAA 1 cut(s) 59
AluBI AGCT 6 cut(s) 74, 90, 326, 403, 456, 462
AluI AGCT 6 cut(s) 74, 90, 326, 403, 456, 462
Alw21I GWGCWC 2 cut(s) 275, 385
Alw26I GTCTC 1 cut(s) 493
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 1 cut(s) 289
ApeKI GCWGC 1 cut(s) 74
ApoI RAATTY 1 cut(s) 253
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 1 cut(s) 290
AsuHPI GGTGA 1 cut(s) 316
AvaI CYCGRG 1 cut(s) 266
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 2 cut(s) 275, 385
BbvI GCAGC 1 cut(s) 61
BccI CCATC 1 cut(s) 253
BcoDI GTCTC 1 cut(s) 493
BfaI CTAG 1 cut(s) 353
BfuAI ACCTGC 2 cut(s) 167, 292
BglII AGATCT 2 cut(s) 109, 495
BisI GCNGC 2 cut(s) 75, 299
BlsI GCNGC 2 cut(s) 76, 300
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 1 cut(s) 290
BmsI GCATC 3 cut(s) 192, 243, 303
BpiI GAAGAC 1 cut(s) 66
BpuEI CTTGAG 2 cut(s) 449, 478
BsaBI GATNNNNATC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 493
BsaJI CCNNGG 1 cut(s) 483
BsaWI WCCGGW 1 cut(s) 220
Bse1I ACTGG 1 cut(s) 442
Bse3DI GCAATG 1 cut(s) 366
Bse8I GATNNNNATC 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 483
BseGI GGATG 1 cut(s) 465
BseJI GATNNNNATC 1 cut(s) 114
BseMI GCAATG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 442
BseXI GCAGC 1 cut(s) 61
BseYI CCCAGC 1 cut(s) 322
BshFI GGCC 1 cut(s) 291
BsiHKAI GWGCWC 2 cut(s) 275, 385
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 1 cut(s) 221
BsmAI GTCTC 1 cut(s) 493
BsmI GAATGC 1 cut(s) 278
BsnI GGCC 1 cut(s) 291
Bso31I GGTCTC 1 cut(s) 493
BsoBI CYCGRG 1 cut(s) 266
Bsp1286I GDGCHC 2 cut(s) 275, 385
Bsp1407I TGTACA 1 cut(s) 188
Bsp143I GATC 2 cut(s) 109, 495
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 1 cut(s) 298
BspANI GGCC 1 cut(s) 291
BspHI TCATGA 1 cut(s) 501
BspMI ACCTGC 2 cut(s) 167, 292
BspTNI GGTCTC 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 366
BsrGI TGTACA 1 cut(s) 188
BsrI ACTGG 1 cut(s) 442
BssECI CCNNGG 1 cut(s) 483
BssMI GATC 2 cut(s) 109, 495
BssT1I CCWWGG 1 cut(s) 483
BstAUI TGTACA 1 cut(s) 188
BstC8I GCNNGC 3 cut(s) 92, 358, 417
BstDSI CCRYGG 1 cut(s) 483
BstF5I GGATG 1 cut(s) 465
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 2 cut(s) 112, 498
BstMAI GTCTC 1 cut(s) 493
BstMBI GATC 2 cut(s) 109, 495
BstV1I GCAGC 1 cut(s) 61
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 2 cut(s) 109, 495
BstYI RGATCY 2 cut(s) 109, 495
BsuRI GGCC 1 cut(s) 291
BtgI CCRYGG 1 cut(s) 483
BtsCI GGATG 1 cut(s) 465
BtsI GCAGTG 1 cut(s) 410
BtsIMutI CAGTG 2 cut(s) 410, 435
BveI ACCTGC 2 cut(s) 167, 292
Cac8I GCNNGC 3 cut(s) 92, 358, 417
CciI TCATGA 1 cut(s) 501
CfoI GCGC 1 cut(s) 128
Cfr13I GGNCC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 189
CviAII CATG 3 cut(s) 103, 484, 502
CviQI GTAC 1 cut(s) 189
DpnI GATC 2 cut(s) 111, 497
DpnII GATC 2 cut(s) 109, 495
Eco130I CCWWGG 1 cut(s) 483
Eco31I GGTCTC 1 cut(s) 493
Eco88I CYCGRG 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 483
FaeI CATG 3 cut(s) 106, 487, 505
FaiI YATR 9 cut(s) 50, 104, 153, 287, 311, 390, 453, 485, 503
FatI CATG 3 cut(s) 102, 483, 501
Fnu4HI GCNGC 2 cut(s) 75, 299
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 2 cut(s) 75, 299
FspBI CTAG 1 cut(s) 353
GlaI GCGC 1 cut(s) 127
GluI GCNGC 2 cut(s) 75, 299
GsaI CCCAGC 1 cut(s) 326
HaeIII GGCC 1 cut(s) 291
HapII CCGG 1 cut(s) 221
HhaI GCGC 1 cut(s) 128
Hin1II CATG 3 cut(s) 106, 487, 505
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 1 cut(s) 13
HpaII CCGG 1 cut(s) 221
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 2 cut(s) 191, 435
Hpy188I TCNGA 1 cut(s) 408
Hpy188III TCNNGA 3 cut(s) 268, 428, 502
Hpy8I GTNNAC 2 cut(s) 191, 435
HpyCH4V TGCA 3 cut(s) 161, 344, 476
Hsp92II CATG 3 cut(s) 106, 487, 505
HspAI GCGC 1 cut(s) 126
Kzo9I GATC 2 cut(s) 109, 495
LmnI GCTCC 1 cut(s) 270
LpnPI CCDG 9 cut(s) 2, 162, 199, 233, 234, 287, 336, 401, 423
Lsp1109I GCAGC 1 cut(s) 61
LweI GCATC 3 cut(s) 192, 243, 303
MaeI CTAG 1 cut(s) 353
MaeIII GTNAC 1 cut(s) 304
MalI GATC 2 cut(s) 111, 497
MboI GATC 2 cut(s) 109, 495
MboII GAAGA 6 cut(s) 40, 46, 71, 95, 119, 490
MfeI CAATTG 1 cut(s) 121
MflI RGATCY 2 cut(s) 109, 495
MhlI GDGCHC 2 cut(s) 275, 385
MluCI AATT 4 cut(s) 121, 162, 253, 444
MnlI CCTC 1 cut(s) 46
MseI TTAA 4 cut(s) 114, 131, 216, 526
MspA1I CMGCKG 1 cut(s) 298
MspI CCGG 1 cut(s) 221
MunI CAATTG 1 cut(s) 121
Mva1269I GAATGC 1 cut(s) 278
NcoI CCATGG 1 cut(s) 483
NdeII GATC 2 cut(s) 109, 495
NlaIII CATG 3 cut(s) 106, 487, 505
NmuCI GTSAC 1 cut(s) 304
PagI TCATGA 1 cut(s) 501
PaqCI CACCTGC 1 cut(s) 292
PctI GAATGC 1 cut(s) 278
PfeI GAWTC 1 cut(s) 13
PkrI GCNGC 2 cut(s) 76, 300
PspFI CCCAGC 1 cut(s) 322
PspPI GGNCC 1 cut(s) 290
PsuI RGATCY 2 cut(s) 109, 495
RsaI GTAC 1 cut(s) 190
RsaNI GTAC 1 cut(s) 189
SaqAI TTAA 4 cut(s) 114, 131, 216, 526
SatI GCNGC 2 cut(s) 75, 299
Sau3AI GATC 2 cut(s) 109, 495
Sau96I GGNCC 1 cut(s) 290
SduI GDGCHC 2 cut(s) 275, 385
SfaNI GCATC 3 cut(s) 192, 243, 303
SmlI CTYRAG 2 cut(s) 428, 457
SmoI CTYRAG 2 cut(s) 428, 457
Sse9I AATT 4 cut(s) 121, 162, 253, 444
SsiI CCGC 1 cut(s) 298
SspMI CTAG 1 cut(s) 353
StyI CCWWGG 1 cut(s) 483
TaqI TCGA 1 cut(s) 200
TasI AATT 4 cut(s) 121, 162, 253, 444
TatI WGTACW 1 cut(s) 188
TauI GCSGC 1 cut(s) 301
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 4 cut(s) 114, 131, 216, 526
Tru9I TTAA 4 cut(s) 114, 131, 216, 526
TscAI CASTG 2 cut(s) 417, 442
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 1 cut(s) 74
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 5 cut(s) 26, 47, 91, 96, 490
TspRI CASTG 2 cut(s) 417, 442
XapI RAATTY 1 cut(s) 253
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.