AT5G24340

3'-5' exonuclease domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005335
Physical Location & Seq
Forward (+)
8297280 .. 8300502
3223 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G24340.1

Sequence Viewer

Length: 1518 bp
ATGGAGACCAATCTAAAGATCTATCTAGTTTCATCCACCGACTCGTCCGAGTTCACTCACCTGAAATGGTCTTTCACTCGTTCTACGATCATCGCCTTAGACGCCGAATGGAAGCCACAACACTCCAATACGTCGTCGTTTCCGACCGTCACTCTCCTCCAAGTCGCATGCCGACTCAGTCACGCCACGGATGTCTCCGATGTCTTCCTCATTGATTTGAGTTCGATTCATCTTCCATCGGTTTGGGAGCTGTTGAATGATATGTTCGTGTCGCCGGATGTTCTGAAACTAGGGTTTCGGTTTAAACAGGATTTGGTTTACTTGTCTTCGACATTTACTCAACATGGATGTGAAGGTGGATTCCAAGAGGTGAAACAATACTTGGATATTACAAGCATATACAATTATCTGCAACATAAGCGGTTTGGGAGAAAGGCGCCAAAGGATATCAAGAGCTTGGCTGCTATATGTAAGGAAATGCTGGACATCTCTCTCTCAAAGGAACTTCAATGTAGTGATTGGTCATATCGTCCTCTTACAGAAGAACAGAAACTATACGCTGCCACAGATGCTCACTGCCTGCTCCAGATATTCGATGTATTTGAGGCGCATCTTGTTGAAGGAATCACAGTGCAAGATCTTAGAGTGATAAATGTTGGCTTACAAGAAATTCTGACTGAATCGGACTATAGCAGTAAGATTGTCACAGTCAAACTTTGCAAGGCTACAGATGTAATCAGATCAATGTCGGAAAATGGTCAAAACATAGCCAATGGAGTGGTTCCAAGAAAAACGACACTAAACACGATGCCAATGGATGAGAATTTGTTGAAGATTGTCAGGAAGTTTGGAGAACGGATCCTGTTGAAGGAGTCTGATCTTCTACCAAAGAAACTTAAGAAGAAAACAAGAAGACGTGTCGCCTCAAGCACTATGAACACAAATAAGCAGTTGGTCTGTTCTGCGGACTGGCAAGGTCCACCGCCATGGGACTCATCTTTAGGCGGTGATGGCTGCCCTAAATTTCTATTGGATGTGATGGTTGAAGGTTTGGCGAAACATCTACGTTGTGTGGGGATTGATGCTGCAATCCCACACTCAAAGAAGCCGGATTCAAGGGAGTTGCTTGATCAAGCATTCAAAGAGAACAGAGTTCTATTAACAAGAGATACAAAATTGTTGAGACACCAGGATTTGGCAAAGCATCAAATATATCGAGTAAAGAGTCTTCTTAAAAATGAGCAGCTACTTGAGGTGATAGAGACTTTCCAGCTAAAGATCAGCGGAAATCAGCTGATGTCCAGATGTACGAAGTGCAATGGGAAATTTATTCAGAAACCTCTAAGCATTGAAGAAGCTATTGAAGCAGCAAAGGGTTTCCAAAGAATACCCAACTGCTTATTTAACAAAAATTTAGAGTTTTGGCAGTGCATGAACTGCCATCAACTATACTGGGAGGGAACTCAGTATCATAACGCAGTCCAGAAGTTCATGGAAGTATGCAAGTTGAGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1 Mut7-C

Protein Analysis

505

Amino Acids

57.7

Weight (kDa)

8.48

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 12 - 203 4.3e-20 3'-5' exonuclease
Mut7-C PF01927 340 - 496 2.7e-41 Mut7-C RNAse domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 436
AccB7I CCANNNNNTGG 1 cut(s) 1195
AciI CCGC 5 cut(s) 421, 965, 983, 1005, 1284
AclWI GGATC 2 cut(s) 853, 866
AcsI RAATTY 5 cut(s) 669, 823, 1022, 1325, 1411
AcyI GRCGYC 2 cut(s) 102, 437
AfaI GTAC 1 cut(s) 1309
AfiI CCNNNNNNNGG 2 cut(s) 868, 1195
AflII CTTAAG 1 cut(s) 896
AflIII ACRYGT 1 cut(s) 916
AjiI CACGTC 1 cut(s) 917
AjnI CCWGG 1 cut(s) 1188
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 6 cut(s) 250, 456, 1246, 1273, 1294, 1358
AluI AGCT 6 cut(s) 250, 456, 1246, 1273, 1294, 1358
Alw26I GTCTC 3 cut(s) 199, 1177, 1256
AlwI GGATC 2 cut(s) 853, 866
ApeKI GCWGC 6 cut(s) 461, 560, 1014, 1085, 1243, 1367
ApoI RAATTY 5 cut(s) 669, 823, 1022, 1325, 1411
AspLEI GCGC 2 cut(s) 439, 610
AspS9I GGNCC 1 cut(s) 977
AsuHPI GGTGA 4 cut(s) 50, 382, 1019, 1267
AvaII GGWCC 1 cut(s) 977
BamHI GGATCC 1 cut(s) 858
BanI GGYRCC 1 cut(s) 436
BarI GAAGNNNNNNTAC 2 cut(s) 1212, 1244
BbsI GAAGAC 4 cut(s) 196, 318, 919, 1220
BbvI GCAGC 6 cut(s) 448, 547, 1001, 1072, 1255, 1379
BccI CCATC 4 cut(s) 244, 1004, 1033, 1449
BciT130I CCWGG 1 cut(s) 1190
BclI TGATCA 1 cut(s) 1129
BcoDI GTCTC 3 cut(s) 199, 1177, 1256
BfaI CTAG 2 cut(s) 26, 290
BfmI CTRYAG 2 cut(s) 688, 726
BfoI RGCGCY 1 cut(s) 440
BfrI CTTAAG 1 cut(s) 896
BglII AGATCT 2 cut(s) 18, 637
BisI GCNGC 6 cut(s) 462, 561, 1015, 1086, 1244, 1368
BlsI GCNGC 6 cut(s) 463, 562, 1016, 1087, 1245, 1369
Bme1390I CCNGG 1 cut(s) 1190
Bme18I GGWCC 1 cut(s) 977
BmgBI CACGTC 1 cut(s) 917
BmgT120I GGNCC 1 cut(s) 977
BmiI GGNNCC 3 cut(s) 438, 783, 860
BmrFI CCNGG 1 cut(s) 1190
BmrI ACTGGG 1 cut(s) 1462
BmsI GCATC 5 cut(s) 559, 619, 798, 1072, 1213
BmuI ACTGGG 1 cut(s) 1462
BpiI GAAGAC 4 cut(s) 196, 318, 919, 1220
BpmI CTGGAG 1 cut(s) 569
BpuEI CTTGAG 2 cut(s) 910, 1271
BsaHI GRCGYC 2 cut(s) 102, 437
BsaJI CCNNGG 2 cut(s) 186, 986
Bsc4I CCNNNNNNNGG 2 cut(s) 868, 1195
Bse1I ACTGG 2 cut(s) 974, 1457
Bse3DI GCAATG 1 cut(s) 1324
BseBI CCWGG 1 cut(s) 1190
BseDI CCNNGG 2 cut(s) 186, 986
BseGI GGATG 6 cut(s) 32, 196, 283, 353, 823, 1039
BseLI CCNNNNNNNGG 2 cut(s) 868, 1195
BseMI GCAATG 1 cut(s) 1324
BseMII CTCAG 2 cut(s) 190, 1478
BseNI ACTGG 2 cut(s) 974, 1457
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 6 cut(s) 448, 547, 1001, 1072, 1255, 1379
Bsh1285I CGRYCG 1 cut(s) 147
BshNI GGYRCC 1 cut(s) 436
BsiEI CGRYCG 1 cut(s) 147
BsiSI CCGG 2 cut(s) 275, 1109
BslFI GGGAC 1 cut(s) 1004
BslI CCNNNNNNNGG 2 cut(s) 868, 1195
BsmAI GTCTC 3 cut(s) 199, 1177, 1256
BsmFI GGGAC 1 cut(s) 1004
BsmI GAATGC 1 cut(s) 1136
Bsp143I GATC 8 cut(s) 18, 87, 637, 740, 858, 877, 1129, 1278
Bsp19I CCATGG 1 cut(s) 986
BspACI CCGC 5 cut(s) 421, 965, 983, 1005, 1284
BspCNI CTCAG 2 cut(s) 189, 1477
BspLI GGNNCC 3 cut(s) 438, 783, 860
BspPI GGATC 2 cut(s) 853, 866
BspT107I GGYRCC 1 cut(s) 436
BspTI CTTAAG 1 cut(s) 896
BsrDI GCAATG 1 cut(s) 1324
BsrI ACTGG 2 cut(s) 974, 1457
BssECI CCNNGG 2 cut(s) 186, 986
BssMI GATC 8 cut(s) 18, 87, 637, 740, 858, 877, 1129, 1278
BssNI GRCGYC 2 cut(s) 102, 437
BssT1I CCWWGG 1 cut(s) 986
Bst2UI CCWGG 1 cut(s) 1190
Bst4CI ACNGT 3 cut(s) 148, 631, 709
BstACI GRCGYC 2 cut(s) 102, 437
BstAFI CTTAAG 1 cut(s) 896
BstAPI GCANNNNNTGC 1 cut(s) 1437
BstC8I GCNNGC 2 cut(s) 169, 581
BstDEI CTNAG 5 cut(s) 97, 176, 641, 1343, 1464
BstDSI CCRYGG 2 cut(s) 186, 986
BstENI CCTNNNNNAGG 1 cut(s) 866
BstF5I GGATG 6 cut(s) 32, 196, 283, 353, 823, 1039
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 2 cut(s) 439, 610
BstKTI GATC 8 cut(s) 21, 90, 640, 743, 861, 880, 1132, 1281
BstMAI GTCTC 3 cut(s) 199, 1177, 1256
BstMBI GATC 8 cut(s) 18, 87, 637, 740, 858, 877, 1129, 1278
BstMCI CGRYCG 1 cut(s) 147
BstMWI GCNNNNNNNGC 6 cut(s) 101, 418, 569, 1011, 1364, 1437
BstNI CCWGG 1 cut(s) 1190
BstNSI RCATGY 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 1188
BstSFI CTRYAG 2 cut(s) 688, 726
BstV1I GCAGC 6 cut(s) 448, 547, 1001, 1072, 1255, 1379
BstV2I GAAGAC 4 cut(s) 196, 318, 919, 1220
BstX2I RGATCY 3 cut(s) 18, 637, 858
BstXI CCANNNNNNTGG 3 cut(s) 243, 778, 987
BstYI RGATCY 3 cut(s) 18, 637, 858
BtgI CCRYGG 2 cut(s) 186, 986
BtgZI GCGATG 1 cut(s) 76
BtrI CACGTC 1 cut(s) 917
BtsCI GGATG 6 cut(s) 32, 196, 283, 353, 823, 1039
BtsI GCAGTG 2 cut(s) 574, 1433
BtsIMutI CAGTG 3 cut(s) 574, 636, 1433
Cac8I GCNNGC 2 cut(s) 169, 581
CfoI GCGC 2 cut(s) 439, 610
Cfr13I GGNCC 1 cut(s) 977
CseI GACGC 1 cut(s) 110
Csp6I GTAC 1 cut(s) 1308
CviAII CATG 5 cut(s) 168, 344, 987, 1432, 1492
CviQI GTAC 1 cut(s) 1308
DdeI CTNAG 5 cut(s) 97, 176, 641, 1343, 1464
DinI GGCGCC 1 cut(s) 438
DpnI GATC 8 cut(s) 20, 89, 639, 742, 860, 879, 1131, 1280
DpnII GATC 8 cut(s) 18, 87, 637, 740, 858, 877, 1129, 1278
DraI TTTAAA 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 986
Eco32I GATATC 1 cut(s) 448
Eco47I GGWCC 1 cut(s) 977
EcoNI CCTNNNNNAGG 1 cut(s) 866
EcoRII CCWGG 1 cut(s) 1188
EcoRV GATATC 1 cut(s) 448
EcoT14I CCWWGG 1 cut(s) 986
EgeI GGCGCC 1 cut(s) 438
EheI GGCGCC 1 cut(s) 438
ErhI CCWWGG 1 cut(s) 986
FaeI CATG 5 cut(s) 171, 347, 990, 1435, 1495
FaqI GGGAC 1 cut(s) 1004
FatI CATG 5 cut(s) 167, 343, 986, 1431, 1491
FbaI TGATCA 1 cut(s) 1129
Fnu4HI GCNGC 6 cut(s) 462, 561, 1015, 1086, 1244, 1368
FokI GGATG 6 cut(s) 19, 203, 290, 360, 830, 1046
Fsp4HI GCNGC 6 cut(s) 462, 561, 1015, 1086, 1244, 1368
FspBI CTAG 2 cut(s) 26, 290
GlaI GCGC 2 cut(s) 438, 609
GluI GCNGC 6 cut(s) 462, 561, 1015, 1086, 1244, 1368
GsuI CTGGAG 1 cut(s) 569
HaeII RGCGCY 1 cut(s) 440
HapII CCGG 2 cut(s) 275, 1109
HgaI GACGC 1 cut(s) 110
HhaI GCGC 2 cut(s) 439, 610
Hin1I GRCGYC 2 cut(s) 102, 437
Hin1II CATG 5 cut(s) 171, 347, 990, 1435, 1495
Hin6I GCGC 2 cut(s) 437, 608
HinP1I GCGC 2 cut(s) 437, 608
HpaII CCGG 2 cut(s) 275, 1109
HphI GGTGA 4 cut(s) 50, 382, 1019, 1267
Hpy166II GTNNAC 3 cut(s) 54, 319, 980
Hpy188III TCNNGA 5 cut(s) 451, 586, 841, 1302, 1483
Hpy8I GTNNAC 3 cut(s) 54, 319, 980
Hpy99I CGWCG 2 cut(s) 136, 139
HpyAV CCTTC 4 cut(s) 347, 614, 862, 1040
HpyCH4III ACNGT 3 cut(s) 148, 631, 709
HpyCH4IV ACGT 3 cut(s) 131, 916, 1066
HpyCH4V TGCA 7 cut(s) 412, 634, 720, 1088, 1317, 1431, 1503
HpyF10VI GCNNNNNNNGC 6 cut(s) 101, 418, 569, 1011, 1364, 1437
HpyF3I CTNAG 5 cut(s) 97, 176, 641, 1343, 1464
HpySE526I ACGT 3 cut(s) 131, 916, 1066
Hsp92I GRCGYC 2 cut(s) 102, 437
Hsp92II CATG 5 cut(s) 171, 347, 990, 1435, 1495
HspAI GCGC 2 cut(s) 437, 608
KasI GGCGCC 1 cut(s) 436
Ksp22I TGATCA 1 cut(s) 1129
Kzo9I GATC 8 cut(s) 18, 87, 637, 740, 858, 877, 1129, 1278
LmnI GCTCC 2 cut(s) 247, 588
Lsp1109I GCAGC 6 cut(s) 448, 547, 1001, 1072, 1255, 1379
LweI GCATC 5 cut(s) 559, 619, 798, 1072, 1213
MaeI CTAG 2 cut(s) 26, 290
MaeII ACGT 3 cut(s) 131, 916, 1066
MaeIII GTNAC 3 cut(s) 148, 179, 703
MalI GATC 8 cut(s) 20, 89, 639, 742, 860, 879, 1131, 1280
MboI GATC 8 cut(s) 18, 87, 637, 740, 858, 877, 1129, 1278
MflI RGATCY 3 cut(s) 18, 637, 858
MluCI AATT 7 cut(s) 403, 669, 823, 1022, 1175, 1325, 1411
Mly113I GGCGCC 1 cut(s) 437
MlyI GAGTC 5 cut(s) 35, 168, 881, 986, 1234
MmeI TCCRAC 2 cut(s) 167, 729
MnlI CCTC 9 cut(s) 167, 218, 361, 543, 598, 934, 1246, 1350, 1450
MseI TTAA 5 cut(s) 303, 897, 1160, 1233, 1404
MslI CAYNNNNRTG 2 cut(s) 348, 985
MspA1I CMGCKG 2 cut(s) 1284, 1294
MspCI CTTAAG 1 cut(s) 896
MspI CCGG 2 cut(s) 275, 1109
MspR9I CCNGG 1 cut(s) 1190
MssI GTTTAAAC 1 cut(s) 304
Mva1269I GAATGC 1 cut(s) 1136
MvaI CCWGG 1 cut(s) 1190
MwoI GCNNNNNNNGC 6 cut(s) 101, 418, 569, 1011, 1364, 1437
NarI GGCGCC 1 cut(s) 437
NcoI CCATGG 1 cut(s) 986
NdeII GATC 8 cut(s) 18, 87, 637, 740, 858, 877, 1129, 1278
NlaIII CATG 5 cut(s) 171, 347, 990, 1435, 1495
NlaIV GGNNCC 3 cut(s) 438, 783, 860
NmuCI GTSAC 3 cut(s) 148, 179, 703
NspI RCATGY 1 cut(s) 171
PaeI GCATGC 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 140
PctI GAATGC 1 cut(s) 1136
PfeI GAWTC 5 cut(s) 226, 360, 624, 680, 1112
PflFI GACNNNGTC 1 cut(s) 177
PflMI CCANNNNNTGG 1 cut(s) 1195
PkrI GCNGC 6 cut(s) 463, 562, 1016, 1087, 1245, 1369
PleI GAGTC 5 cut(s) 35, 168, 880, 986, 1233
PluTI GGCGCC 1 cut(s) 440
PmeI GTTTAAAC 1 cut(s) 304
PpsI GAGTC 5 cut(s) 35, 168, 880, 986, 1233
Psp6I CCWGG 1 cut(s) 1188
PspGI CCWGG 1 cut(s) 1188
PspN4I GGNNCC 3 cut(s) 438, 783, 860
PspPI GGNCC 1 cut(s) 977
PsuI RGATCY 3 cut(s) 18, 637, 858
PsyI GACNNNGTC 1 cut(s) 177
PvuII CAGCTG 1 cut(s) 1294
RsaI GTAC 1 cut(s) 1309
RsaNI GTAC 1 cut(s) 1308
RseI CAYNNNNRTG 2 cut(s) 348, 985
SaqAI TTAA 5 cut(s) 303, 897, 1160, 1233, 1404
SatI GCNGC 6 cut(s) 462, 561, 1015, 1086, 1244, 1368
Sau3AI GATC 8 cut(s) 18, 87, 637, 740, 858, 877, 1129, 1278
Sau96I GGNCC 1 cut(s) 977
SchI GAGTC 5 cut(s) 35, 168, 881, 986, 1234
ScrFI CCNGG 1 cut(s) 1190
SfaNI GCATC 5 cut(s) 559, 619, 798, 1072, 1213
SfcI CTRYAG 2 cut(s) 688, 726
SfoI GGCGCC 1 cut(s) 438
SinI GGWCC 1 cut(s) 977
SmiMI CAYNNNNRTG 2 cut(s) 348, 985
SmlI CTYRAG 3 cut(s) 896, 925, 1250
SmoI CTYRAG 3 cut(s) 896, 925, 1250
SphI GCATGC 1 cut(s) 171
Sse9I AATT 7 cut(s) 403, 669, 823, 1022, 1175, 1325, 1411
SsiI CCGC 5 cut(s) 421, 965, 983, 1005, 1284
SspDI GGCGCC 1 cut(s) 436
SspMI CTAG 2 cut(s) 26, 290
StyD4I CCNGG 1 cut(s) 1188
StyI CCWWGG 1 cut(s) 986
TaaI ACNGT 3 cut(s) 148, 631, 709
TaiI ACGT 3 cut(s) 134, 919, 1069
TaqI TCGA 4 cut(s) 224, 329, 594, 1216
TasI AATT 7 cut(s) 403, 669, 823, 1022, 1175, 1325, 1411
TfiI GAWTC 5 cut(s) 226, 360, 624, 680, 1112
Tru1I TTAA 5 cut(s) 303, 897, 1160, 1233, 1404
Tru9I TTAA 5 cut(s) 303, 897, 1160, 1233, 1404
TscAI CASTG 3 cut(s) 581, 636, 1433
TseFI GTSAC 3 cut(s) 148, 179, 703
TseI GCWGC 6 cut(s) 461, 560, 1014, 1085, 1243, 1367
Tsp45I GTSAC 3 cut(s) 148, 179, 703
TspDTI ATGAA 5 cut(s) 21, 218, 950, 1448, 1480
TspGWI ACGGA 2 cut(s) 203, 871
TspRI CASTG 3 cut(s) 581, 636, 1433
Tth111I GACNNNGTC 1 cut(s) 177
Van91I CCANNNNNTGG 1 cut(s) 1195
Vha464I CTTAAG 1 cut(s) 896
VpaK11BI GGWCC 1 cut(s) 977
XagI CCTNNNNNAGG 1 cut(s) 866
XapI RAATTY 5 cut(s) 669, 823, 1022, 1325, 1411
XceI RCATGY 1 cut(s) 171
XspI CTAG 2 cut(s) 26, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.