AT5G24900

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004647
Physical Location & Seq
Reverse (-)
8563800 .. 8566812
3013 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G24900.1

Sequence Viewer

Length: 1578 bp
ATGGAGAGTTTGGTTGTTCATACGGTAAATGCAATTTGGTGCATAGTTATTGTCGGAATCTTCAGCGTAGGTTATCATGTGTATGGAAGAGCGGTGGTGGAGCAGTGGAGGATGCGGAGGAGTTTAAAGTTGCAAGGCGTGAAGGGTCCTCCACCGTCGATCTTTAACGGCAATGTGTCGGAGATGCAACGGATTCAGTCGGAGGCTAAACACTGTTCCGGCGATAACATCATTTCTCATGACTATTCTTCTTCTCTATTTCCTCATTTCGATCACTGGCGAAAACAATACGGAAGGATTTACACATACTCAACGGGGTTAAAGCAGCACCTTTACATAAACCACCCGGAAATGGTGAAGGAGCTTAGCCAAACCAACACACTTAACCTTGGTAGAATCACTCACATCACCAAACGCCTTAACCCCATTCTCGGCAATGGCATCATCACCTCTAATGGGCCTCATTGGGCCCATCAACGTCGTATCATTGCCTATGAGTTTACCCACGACAAAATCAAGGGAATGGTTGGTTTAATGGTGGAATCTGCCATGCCAATGTTGAACAAATGGGAAGAGATGGTGAAAAGAGGAGGAGAAATGGGTTGTGACATAAGAGTGGACGAAGACCTTAAGGATGTCTCAGCTGATGTCATCGCTAAGGCTTGCTTTGGGAGCTCTTTTTCAAAAGGCAAAGCAATATTCTCTATGATTAGGGATCTTTTAACCGCCATTACTAAACGAAGCGTCCTCTTCAGATTCAATGGCTTCACTGATATGGTGTTTGGAAGTAAGAAGCATGGTGATGTGGATATTGATGCGCTTGAGATGGAATTAGAATCTTCTATATGGGAAACGGTTAAGGAGAGGGAAATTGAATGTAAGGATACTCACAAGAAGGATCTAATGCAGTTGATACTCGAGGGAGCGATGCGAAGCTGCGATGGTAACTTGTGGGACAAGTCAGCCTATAGACGGTTTGTGGTGGACAATTGCAAGAGCATCTATTTCGCCGGACATGATTCAACCGCAGTCTCAGTGTCTTGGTGCCTTATGCTCCTCGCTCTCAATCCTAGTTGGCAGGTTAAAATTCGCGATGAAATCTTGAGTTCTTGCAAGAATGGCATTCCCGACGCAGAATCAATTCCTAATCTCAAAACGGTGACAATGGTAATACAAGAAACAATGAGACTATACCCACCAGCACCAATCGTGGGAAGAGAAGCATCCAAAGACATAAGACTTGGAGACCTTGTGGTGCCAAAAGGAGTGTGCATTTGGACACTCATTCCTGCCTTACACCGAGACCCCGAGATCTGGGGACCAGACGCAAACGACTTCAAGCCAGAGAGGTTTAGTGAGGGAATCTCTAAGGCTTGCAAATACCCTCAGTCATACATCCCATTTGGCCTTGGACCAAGAACATGCGTAGGCAAAAACTTTGGTATGATGGAAGTGAAAGTGCTTGTTTCACTTATTGTCTCAAAGTTCAGTTTTACTCTTTCCCCGACTTATCAGCACTCTCCAAGCCATAAACTCCTTGTAGAGCCTCAACATGGTGTTGTCATTAGGGTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

525

Amino Acids

59.33

Weight (kDa)

8.75

Isoelectric Point (pI)

33.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 85 - 514 3.6e-78 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1069
AccB1I GGYRCC 2 cut(s) 1044, 1255
AccBSI CCGCTC 1 cut(s) 92
AccII CGCG 1 cut(s) 1092
AciI CCGC 4 cut(s) 92, 115, 726, 1026
AclWI GGATC 2 cut(s) 723, 906
AcsI RAATTY 1 cut(s) 1086
AcuI CTGAAG 2 cut(s) 46, 736
AfiI CCNNNNNNNGG 7 cut(s) 352, 431, 456, 972, 1211, 1314, 1553
AflII CTTAAG 1 cut(s) 629
AgsI TTSAA 6 cut(s) 562, 684, 760, 875, 1023, 1339
AluBI AGCT 4 cut(s) 364, 644, 675, 936
AluI AGCT 4 cut(s) 364, 644, 675, 936
Alw21I GWGCWC 1 cut(s) 677
Alw26I GTCTC 6 cut(s) 643, 1036, 1180, 1239, 1296, 1483
AlwI GGATC 2 cut(s) 723, 906
Ama87I CYCGRG 2 cut(s) 917, 1307
AoxI GGCC 3 cut(s) 458, 468, 1405
ApaI GGGCCC 1 cut(s) 472
ApeKI GCWGC 2 cut(s) 325, 936
ApoI RAATTY 1 cut(s) 1086
Asp700I GAANNNNTTC 1 cut(s) 1140
AspLEI GCGC 1 cut(s) 820
AspS9I GGNCC 6 cut(s) 146, 458, 468, 469, 1319, 1412
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 6 cut(s) 367, 400, 439, 592, 812, 1171
AvaI CYCGRG 2 cut(s) 917, 1307
AvaII GGWCC 3 cut(s) 146, 1319, 1412
BaeGI GKGCMC 1 cut(s) 472
BanI GGYRCC 2 cut(s) 1044, 1255
BanII GRGCYC 2 cut(s) 472, 677
BbsI GAAGAC 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 677
BbvI GCAGC 2 cut(s) 337, 923
BccI CCATC 5 cut(s) 480, 571, 820, 935, 1441
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 2 cut(s) 988, 1022
BciVI GTATCC 1 cut(s) 877
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 6 cut(s) 643, 1036, 1180, 1239, 1296, 1483
BfaI CTAG 1 cut(s) 1071
BfmI CTRYAG 1 cut(s) 967
BfrI CTTAAG 1 cut(s) 629
BfuAI ACCTGC 1 cut(s) 1069
BfuI GTATCC 1 cut(s) 877
BglII AGATCT 1 cut(s) 1311
BisI GCNGC 2 cut(s) 326, 937
BlpI GCTNAGC 1 cut(s) 365
BlsI GCNGC 2 cut(s) 327, 938
Bme1390I CCNGG 1 cut(s) 347
Bme18I GGWCC 3 cut(s) 146, 1319, 1412
BmeT110I CYCGRG 2 cut(s) 917, 1307
BmgT120I GGNCC 6 cut(s) 146, 458, 468, 469, 1319, 1412
BmiI GGNNCC 5 cut(s) 147, 470, 1046, 1257, 1320
BmrFI CCNGG 1 cut(s) 347
BmsI GCATC 7 cut(s) 102, 174, 450, 805, 918, 1008, 1232
BpiI GAAGAC 1 cut(s) 630
BplI GAGNNNNNCTC 2 cut(s) 1349, 1381
Bpu10I CCTNAGC 1 cut(s) 657
Bpu1102I GCTNAGC 1 cut(s) 365
BpuEI CTTGAG 2 cut(s) 842, 1123
BpuMI CCSGG 1 cut(s) 347
BsaI GGTCTC 2 cut(s) 1239, 1296
BsaJI CCNNGG 2 cut(s) 388, 1408
BsaXI ACNNNNNCTCC 2 cut(s) 1236, 1266
Bsc4I CCNNNNNNNGG 7 cut(s) 352, 431, 456, 972, 1211, 1314, 1553
Bse1I ACTGG 1 cut(s) 281
Bse3DI GCAATG 3 cut(s) 178, 442, 486
BseDI CCNNGG 2 cut(s) 388, 1408
BseGI GGATG 4 cut(s) 117, 640, 1223, 1395
BseLI CCNNNNNNNGG 7 cut(s) 352, 431, 456, 972, 1211, 1314, 1553
BseMI GCAATG 3 cut(s) 178, 442, 486
BseMII CTCAG 3 cut(s) 654, 1047, 1400
BseNI ACTGG 1 cut(s) 281
BseRI GAGGAG 4 cut(s) 133, 603, 606, 1046
BseSI GKGCMC 1 cut(s) 472
BseXI GCAGC 2 cut(s) 337, 923
Bsh1236I CGCG 1 cut(s) 1092
BshFI GGCC 3 cut(s) 460, 470, 1407
BshNI GGYRCC 2 cut(s) 1044, 1255
BsiHKAI GWGCWC 1 cut(s) 677
BsiHKCI CYCGRG 2 cut(s) 917, 1307
BsiSI CCGG 3 cut(s) 219, 347, 1011
BslFI GGGAC 2 cut(s) 968, 1332
BslI CCNNNNNNNGG 7 cut(s) 352, 431, 456, 972, 1211, 1314, 1553
BsmAI GTCTC 6 cut(s) 643, 1036, 1180, 1239, 1296, 1483
BsmFI GGGAC 2 cut(s) 968, 1332
BsmI GAATGC 1 cut(s) 1122
BsnI GGCC 3 cut(s) 460, 470, 1407
Bso31I GGTCTC 2 cut(s) 1239, 1296
BsoBI CYCGRG 2 cut(s) 917, 1307
Bsp120I GGGCCC 1 cut(s) 468
Bsp1286I GDGCHC 2 cut(s) 472, 677
Bsp143I GATC 5 cut(s) 159, 271, 715, 898, 1311
Bsp1720I GCTNAGC 1 cut(s) 365
Bsp68I TCGCGA 1 cut(s) 1092
BspACI CCGC 4 cut(s) 92, 115, 726, 1026
BspANI GGCC 3 cut(s) 460, 470, 1407
BspCNI CTCAG 3 cut(s) 653, 1046, 1399
BspFNI CGCG 1 cut(s) 1092
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 5 cut(s) 147, 470, 1046, 1257, 1320
BspMI ACCTGC 1 cut(s) 1069
BspPI GGATC 2 cut(s) 723, 906
BspQI GCTCTTC 1 cut(s) 82
BspT107I GGYRCC 2 cut(s) 1044, 1255
BspTI CTTAAG 1 cut(s) 629
BspTNI GGTCTC 2 cut(s) 1239, 1296
BsrBI CCGCTC 1 cut(s) 92
BsrDI GCAATG 3 cut(s) 178, 442, 486
BsrI ACTGG 1 cut(s) 281
BssECI CCNNGG 2 cut(s) 388, 1408
BssMI GATC 5 cut(s) 159, 271, 715, 898, 1311
BssT1I CCWWGG 2 cut(s) 388, 1408
Bst4CI ACNGT 6 cut(s) 25, 156, 215, 856, 975, 1159
Bst6I CTCTTC 4 cut(s) 82, 567, 755, 1210
BstAFI CTTAAG 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 664, 1375
BstDEI CTNAG 6 cut(s) 365, 640, 657, 1033, 1368, 1386
BstF5I GGATG 4 cut(s) 117, 640, 1223, 1395
BstFNI CGCG 1 cut(s) 1092
BstHHI GCGC 1 cut(s) 820
BstKTI GATC 5 cut(s) 162, 274, 718, 901, 1314
BstMAI GTCTC 6 cut(s) 643, 1036, 1180, 1239, 1296, 1483
BstMBI GATC 5 cut(s) 159, 271, 715, 898, 1311
BstMWI GCNNNNNNNGC 2 cut(s) 672, 1119
BstNSI RCATGY 1 cut(s) 1425
BstSCI CCNGG 1 cut(s) 345
BstSFI CTRYAG 1 cut(s) 967
BstSLI GKGCMC 1 cut(s) 472
BstUI CGCG 1 cut(s) 1092
BstV1I GCAGC 2 cut(s) 337, 923
BstV2I GAAGAC 1 cut(s) 630
BstX2I RGATCY 3 cut(s) 715, 898, 1311
BstYI RGATCY 3 cut(s) 715, 898, 1311
BsuI GTATCC 1 cut(s) 877
BsuRI GGCC 3 cut(s) 460, 470, 1407
BtgZI GCGATG 4 cut(s) 637, 941, 954, 1107
BtsCI GGATG 4 cut(s) 117, 640, 1223, 1395
BtsI GCAGTG 1 cut(s) 110
BtsIMutI CAGTG 5 cut(s) 110, 211, 274, 768, 1041
BtuMI TCGCGA 1 cut(s) 1092
BveI ACCTGC 1 cut(s) 1069
Cac8I GCNNGC 2 cut(s) 664, 1375
CciI TCATGA 1 cut(s) 238
CfoI GCGC 1 cut(s) 820
Cfr13I GGNCC 6 cut(s) 146, 458, 468, 469, 1319, 1412
CseI GACGC 3 cut(s) 733, 1139, 1334
CviAII CATG 7 cut(s) 77, 239, 550, 797, 1016, 1422, 1553
DdeI CTNAG 6 cut(s) 365, 640, 657, 1033, 1368, 1386
DpnI GATC 5 cut(s) 161, 273, 717, 900, 1313
DpnII GATC 5 cut(s) 159, 271, 715, 898, 1311
DraI TTTAAA 1 cut(s) 126
Eam1104I CTCTTC 4 cut(s) 82, 567, 755, 1210
EarI CTCTTC 4 cut(s) 82, 567, 755, 1210
Ecl136II GAGCTC 1 cut(s) 675
Eco130I CCWWGG 2 cut(s) 388, 1408
Eco24I GRGCYC 2 cut(s) 472, 677
Eco31I GGTCTC 2 cut(s) 1239, 1296
Eco47I GGWCC 3 cut(s) 146, 1319, 1412
Eco53kI GAGCTC 1 cut(s) 675
Eco57I CTGAAG 2 cut(s) 46, 736
Eco88I CYCGRG 2 cut(s) 917, 1307
EcoICRI GAGCTC 1 cut(s) 675
EcoO109I RGGNCCY 1 cut(s) 146
EcoT14I CCWWGG 2 cut(s) 388, 1408
EcoT38I GRGCYC 2 cut(s) 472, 677
ErhI CCWWGG 2 cut(s) 388, 1408
FaeI CATG 7 cut(s) 80, 242, 553, 800, 1019, 1425, 1556
FalI AAGNNNNNCTT 2 cut(s) 650, 682
FaqI GGGAC 2 cut(s) 968, 1332
FatI CATG 7 cut(s) 76, 238, 549, 796, 1015, 1421, 1552
Fnu4HI GCNGC 2 cut(s) 326, 937
FokI GGATG 4 cut(s) 124, 647, 1210, 1382
FriOI GRGCYC 2 cut(s) 472, 677
Fsp4HI GCNGC 2 cut(s) 326, 937
FspBI CTAG 1 cut(s) 1071
GlaI GCGC 1 cut(s) 819
GluI GCNGC 2 cut(s) 326, 937
HaeIII GGCC 3 cut(s) 460, 470, 1407
HapII CCGG 3 cut(s) 219, 347, 1011
HgaI GACGC 3 cut(s) 733, 1139, 1334
HhaI GCGC 1 cut(s) 820
Hin1II CATG 7 cut(s) 80, 242, 553, 800, 1019, 1425, 1556
Hin6I GCGC 1 cut(s) 818
HinP1I GCGC 1 cut(s) 818
HinfI GANTC 9 cut(s) 57, 193, 396, 542, 756, 836, 1019, 1136, 1362
HpaII CCGG 3 cut(s) 219, 347, 1011
HphI GGTGA 6 cut(s) 367, 400, 439, 592, 812, 1171
Hpy166II GTNNAC 3 cut(s) 501, 619, 985
Hpy188I TCNGA 4 cut(s) 56, 181, 202, 755
Hpy188III TCNNGA 4 cut(s) 239, 1091, 1102, 1127
Hpy8I GTNNAC 3 cut(s) 501, 619, 985
Hpy99I CGWCG 3 cut(s) 160, 483, 1133
HpyAV CCTTC 4 cut(s) 136, 288, 352, 889
HpyCH4III ACNGT 6 cut(s) 25, 156, 215, 856, 975, 1159
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 9 cut(s) 32, 42, 133, 187, 907, 993, 1113, 1272, 1377
HpyF10VI GCNNNNNNNGC 2 cut(s) 672, 1119
HpyF3I CTNAG 6 cut(s) 365, 640, 657, 1033, 1368, 1386
HpySE526I ACGT 1 cut(s) 478
Hsp92II CATG 7 cut(s) 80, 242, 553, 800, 1019, 1425, 1556
HspAI GCGC 1 cut(s) 818
Kzo9I GATC 5 cut(s) 159, 271, 715, 898, 1311
LguI GCTCTTC 1 cut(s) 82
LmnI GCTCC 5 cut(s) 100, 361, 672, 923, 1059
Lsp1109I GCAGC 2 cut(s) 337, 923
LweI GCATC 7 cut(s) 102, 174, 450, 805, 918, 1008, 1232
MaeI CTAG 1 cut(s) 1071
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 3 cut(s) 605, 944, 1159
MalI GATC 5 cut(s) 161, 273, 717, 900, 1313
MbiI CCGCTC 1 cut(s) 92
MboI GATC 5 cut(s) 159, 271, 715, 898, 1311
MboII GAAGA 9 cut(s) 52, 99, 240, 243, 584, 635, 742, 831, 1227
MfeI CAATTG 1 cut(s) 988
MflI RGATCY 3 cut(s) 715, 898, 1311
MhlI GDGCHC 2 cut(s) 472, 677
MluCI AATT 6 cut(s) 33, 830, 870, 988, 1086, 1140
MmeI TCCRAC 3 cut(s) 34, 159, 180
MroXI GAANNNNTTC 1 cut(s) 1140
MslI CAYNNNNRTG 5 cut(s) 81, 554, 614, 773, 801
MspA1I CMGCKG 1 cut(s) 644
MspCI CTTAAG 1 cut(s) 629
MspI CCGG 3 cut(s) 219, 347, 1011
MspR9I CCNGG 1 cut(s) 347
MunI CAATTG 1 cut(s) 988
Mva1269I GAATGC 1 cut(s) 1122
MvnI CGCG 1 cut(s) 1092
MwoI GCNNNNNNNGC 2 cut(s) 672, 1119
NciI CCSGG 1 cut(s) 347
NdeII GATC 5 cut(s) 159, 271, 715, 898, 1311
NlaIII CATG 7 cut(s) 80, 242, 553, 800, 1019, 1425, 1556
NlaIV GGNNCC 5 cut(s) 147, 470, 1046, 1257, 1320
NmeAIII GCCGAG 1 cut(s) 411
NmuCI GTSAC 2 cut(s) 605, 1159
NruI TCGCGA 1 cut(s) 1092
NspI RCATGY 1 cut(s) 1425
PaeR7I CTCGAG 1 cut(s) 917
PagI TCATGA 1 cut(s) 238
PciSI GCTCTTC 1 cut(s) 82
PctI GAATGC 1 cut(s) 1122
PdmI GAANNNNTTC 1 cut(s) 1140
PfeI GAWTC 9 cut(s) 57, 193, 396, 542, 756, 836, 1019, 1136, 1362
PkrI GCNGC 2 cut(s) 327, 938
PpuMI RGGWCCY 1 cut(s) 146
Psp124BI GAGCTC 1 cut(s) 677
Psp5II RGGWCCY 1 cut(s) 146
PspN4I GGNNCC 5 cut(s) 147, 470, 1046, 1257, 1320
PspOMI GGGCCC 1 cut(s) 468
PspPI GGNCC 6 cut(s) 146, 458, 468, 469, 1319, 1412
PspPPI RGGWCCY 1 cut(s) 146
PspXI VCTCGAGB 1 cut(s) 917
PsuI RGATCY 3 cut(s) 715, 898, 1311
PvuII CAGCTG 1 cut(s) 644
RruI TCGCGA 1 cut(s) 1092
RseI CAYNNNNRTG 5 cut(s) 81, 554, 614, 773, 801
SacI GAGCTC 1 cut(s) 677
SapI GCTCTTC 1 cut(s) 82
SatI GCNGC 2 cut(s) 326, 937
Sau3AI GATC 5 cut(s) 159, 271, 715, 898, 1311
Sau96I GGNCC 6 cut(s) 146, 458, 468, 469, 1319, 1412
ScrFI CCNGG 1 cut(s) 347
SduI GDGCHC 2 cut(s) 472, 677
SfaNI GCATC 7 cut(s) 102, 174, 450, 805, 918, 1008, 1232
SfcI CTRYAG 1 cut(s) 967
Sfr274I CTCGAG 1 cut(s) 917
SinI GGWCC 3 cut(s) 146, 1319, 1412
SlaI CTCGAG 1 cut(s) 917
SmiMI CAYNNNNRTG 5 cut(s) 81, 554, 614, 773, 801
SmlI CTYRAG 4 cut(s) 629, 821, 917, 1102
SmoI CTYRAG 4 cut(s) 629, 821, 917, 1102
Sse9I AATT 6 cut(s) 33, 830, 870, 988, 1086, 1140
SsiI CCGC 4 cut(s) 92, 115, 726, 1026
SspI AATATT 1 cut(s) 699
SspMI CTAG 1 cut(s) 1071
SstI GAGCTC 1 cut(s) 677
StyD4I CCNGG 1 cut(s) 345
StyI CCWWGG 2 cut(s) 388, 1408
TaaI ACNGT 6 cut(s) 25, 156, 215, 856, 975, 1159
TaiI ACGT 1 cut(s) 481
TaqI TCGA 3 cut(s) 158, 270, 918
TasI AATT 6 cut(s) 33, 830, 870, 988, 1086, 1140
TfiI GAWTC 9 cut(s) 57, 193, 396, 542, 756, 836, 1019, 1136, 1362
TscAI CASTG 5 cut(s) 110, 218, 281, 775, 1041
TseFI GTSAC 2 cut(s) 605, 1159
TseI GCWGC 2 cut(s) 325, 936
Tsp45I GTSAC 2 cut(s) 605, 1159
TspDTI ATGAA 2 cut(s) 8, 1110
TspGWI ACGGA 2 cut(s) 205, 306
TspRI CASTG 5 cut(s) 110, 218, 281, 775, 1041
Vha464I CTTAAG 1 cut(s) 629
VpaK11BI GGWCC 3 cut(s) 146, 1319, 1412
XapI RAATTY 1 cut(s) 1086
XceI RCATGY 1 cut(s) 1425
XhoI CTCGAG 1 cut(s) 917
XmnI GAANNNNTTC 1 cut(s) 1140
XspI CTAG 1 cut(s) 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.