RchiOBHm_Chr1g0372871

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004647
Physical Location & Seq
Reverse (-)
61367810 .. 61371200
3391 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59684

Sequence Viewer

Length: 1578 bp
ATGGAGCTCTCTCTTCATTTCAGAATCGTTTCTTCCCTAGTTCTTTTGGGACTGGTTGGCGTCTTTCTTCATCTCTACAACACAGTCTGGTTGAAGTCCGAGAGGGTCAGAAGGAAGCTCCAAATGCAAGGCATCAAAGGCCCTACACCTTCTTTACTGTATGGGAATCTCGCTGAGATGCAGAAAATAATCCAACTCCAAGCTCAGAAGTCTTCAAATCACTCAGAATTTGTAGCCCACGACTACACTTCCAGTCTCTTCCCCTATTTCGAACACTGGAGAAAAGAATACGGATTAATTTACACGTATTCAACAGGAATGAGGCAGCATTTATATGTGAACCAACCGGAGCTAGTGAAGGAAATGAACCAGTGTATTAGTTTGGATTTAGGTAAGCCTTCATATGTAAGCAAGAGACTTGCTCCCATGCTTGGAAATGGAATTCTCAGATCCAATGGCATTGTTTGGTCACAACAAAGAAAAATTGTTGCACCTGAGTTCTTCACAGACAAAGTCAAGGCGATGGTAGGGTTGATGTTGGAGTCGGCGCAACCATTGCTTAGAAAATGGGAGGAGTGTATTGAAGGACAAGGAGGTGCAAGAGCAGAGATTCAAGTAGATAAGGATTTAAGGGATTTCTCAGCTGATGTTATCTCGAGGGCTTGTTTTGGCAGTTCTTATTCTAAGGGCAAAGAGATTTTCTCCAAGCTTAGAAGTCTTCAGGGAATAATTTCTCATCAACCCTTCCTCTTTGGATCATTTTCTAATTTTGGAGGGTTTAAGAAGCAAAAGGAGATTAGTTGTTTAGAGAGAGAGATAGAGTCACTGATATGGGAAACTGTGAAAGAACGCGAACGAGAAGGGTTAGAGGCTTCTTCGTCGGAAAAGGATCTGCTGCAGTCATTATTAGTGGGAGCCAGGAATGACCAAAGCCTAGGCAAAGGCAAGGATTCATCCAAGCGATTCATAGTTGACAACTGCAAGAACATTTACTTTGCAGGGCACGAATCCACTGGTGCTGCTGCCTCTTGGTGTCTGATGCTACTCTCTTTACATCCCGAGTGGCAAGCTCGAATCAGGACCGAGTTGGCAGAAGTTTTCCCCGATGGTCAGCTAGATGCTAATTCGCTCCCTCAACTGAGAACGATGAATATGGTGATTCAAGAAGTCATGCGTCTATACCCACCAGCTGCTTTTGTGTCCAGAGAAGCACTTGAAGACACCCAGGTGGGAAACATAAGCATTCCAAAAGGGTTATGCATGTGGACTTTGATCCCAACATTGCACAGAGACACAGAGATTTGGGGACAAGATGCAAATGAATTCAAACCTGAGAGGTTCATTGATGGTGTCTCTAAGGCTTGCAAATTTCCTCAGGCCTATATCCCCTTTGGTTTGGGTCCTCGAACCTGTTTAGGCAAAAACTTTGCTATGGTAGAATTGAAGGTTGTCCTCTCCCTCATTATCTCCAAGTTCACTTTCTCTCTCTCTCCCAAATACAGACACTCTCCTGCTTTCGAAATGATTGTAGGTCCTGGAAATGGTGTACATATTCTCATTCAAAAATCTGATATGTAG

Protein Analysis

525

Amino Acids

59.39

Weight (kDa)

8.85

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 85 - 506 1.8e-76 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 852
AclWI GGATC 4 cut(s) 444, 763, 897, 1267
AcsI RAATTY 4 cut(s) 227, 441, 1322, 1367
AcuI CTGAAG 1 cut(s) 704
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 1 cut(s) 1548
AfiI CCNNNNNNNGG 2 cut(s) 431, 1541
AflIII ACRYGT 1 cut(s) 303
AjnI CCWGG 3 cut(s) 917, 1224, 1534
AjuI GAANNNNNNNTTGG 2 cut(s) 1463, 1495
AleI CACNNNNGTG 1 cut(s) 1226
AluBI AGCT 9 cut(s) 7, 118, 203, 352, 644, 709, 1070, 1114, 1190
AluI AGCT 9 cut(s) 7, 118, 203, 352, 644, 709, 1070, 1114, 1190
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 4 cut(s) 260, 409, 1284, 1357
AlwI GGATC 4 cut(s) 444, 763, 897, 1267
Ama87I CYCGRG 2 cut(s) 655, 1058
AoxI GGCC 2 cut(s) 139, 1377
ApeKI GCWGC 5 cut(s) 325, 895, 1019, 1022, 1190
ApoI RAATTY 4 cut(s) 227, 441, 1322, 1367
AseI ATTAAT 1 cut(s) 296
Asp700I GAANNNNTTC 2 cut(s) 28, 730
AspA2I CCTAGG 1 cut(s) 934
AspLEI GCGC 1 cut(s) 550
AspS9I GGNCC 4 cut(s) 140, 1080, 1400, 1532
AsuHPI GGTGA 1 cut(s) 1168
AsuII TTCGAA 2 cut(s) 270, 1518
AvaI CYCGRG 2 cut(s) 655, 1058
AvaII GGWCC 3 cut(s) 1080, 1400, 1532
AvrII CCTAGG 1 cut(s) 934
AxyI CCTNAGG 1 cut(s) 1374
BaeGI GKGCMC 1 cut(s) 1005
BanII GRGCYC 1 cut(s) 9
BbsI GAAGAC 3 cut(s) 204, 710, 1224
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 5 cut(s) 337, 882, 1006, 1009, 1177
BccI CCATC 3 cut(s) 517, 1100, 1340
BciT130I CCWGG 3 cut(s) 919, 1226, 1536
BcoDI GTCTC 4 cut(s) 260, 409, 1284, 1357
BfaI CTAG 4 cut(s) 38, 353, 935, 1115
BfmI CTRYAG 1 cut(s) 896
BisI GCNGC 5 cut(s) 326, 896, 1020, 1023, 1191
BlnI CCTAGG 1 cut(s) 934
BlsI GCNGC 5 cut(s) 327, 897, 1021, 1024, 1192
Bme1390I CCNGG 3 cut(s) 919, 1226, 1536
Bme18I GGWCC 3 cut(s) 1080, 1400, 1532
BmeT110I CYCGRG 2 cut(s) 655, 1058
BmgT120I GGNCC 4 cut(s) 140, 1080, 1400, 1532
BmiI GGNNCC 2 cut(s) 916, 1401
BmrFI CCNGG 3 cut(s) 919, 1226, 1536
BmsI GCATC 5 cut(s) 141, 168, 1029, 1108, 1303
BpiI GAAGAC 3 cut(s) 204, 710, 1224
BplI GAGNNNNNCTC 4 cut(s) 406, 438, 686, 718
BpmI CTGGAG 1 cut(s) 298
Bpu14I TTCGAA 2 cut(s) 270, 1518
BsaAI YACGTR 1 cut(s) 306
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 2 cut(s) 934, 1224
BsaWI WCCGGW 1 cut(s) 346
Bsc4I CCNNNNNNNGG 2 cut(s) 431, 1541
Bse1I ACTGG 5 cut(s) 57, 252, 281, 370, 1018
Bse21I CCTNAGG 1 cut(s) 1374
Bse3DI GCAATG 2 cut(s) 554, 1280
BseBI CCWGG 3 cut(s) 919, 1226, 1536
BseDI CCNNGG 2 cut(s) 934, 1224
BseGI GGATG 2 cut(s) 953, 1054
BseLI CCNNNNNNNGG 2 cut(s) 431, 1541
BseMI GCAATG 2 cut(s) 554, 1280
BseMII CTCAG 9 cut(s) 165, 218, 237, 460, 486, 654, 1130, 1323, 1388
BseNI ACTGG 5 cut(s) 57, 252, 281, 370, 1018
BseRI GAGGAG 1 cut(s) 587
BseSI GKGCMC 1 cut(s) 1005
BseXI GCAGC 5 cut(s) 337, 882, 1006, 1009, 1177
Bsh1236I CGCG 1 cut(s) 852
BshFI GGCC 2 cut(s) 141, 1379
BsiHKAI GWGCWC 1 cut(s) 9
BsiHKCI CYCGRG 2 cut(s) 655, 1058
BsiSI CCGG 1 cut(s) 347
BslFI GGGAC 2 cut(s) 63, 1320
BslI CCNNNNNNNGG 2 cut(s) 431, 1541
BsmAI GTCTC 4 cut(s) 260, 409, 1284, 1357
BsmFI GGGAC 2 cut(s) 63, 1320
BsmI GAATGC 1 cut(s) 1242
BsnI GGCC 2 cut(s) 141, 1379
BsoBI CYCGRG 2 cut(s) 655, 1058
Bsp119I TTCGAA 2 cut(s) 270, 1518
Bsp1286I GDGCHC 2 cut(s) 9, 1005
Bsp1407I TGTACA 1 cut(s) 1546
Bsp143I GATC 4 cut(s) 449, 755, 889, 1272
BspANI GGCC 2 cut(s) 141, 1379
BspCNI CTCAG 9 cut(s) 166, 217, 236, 459, 487, 653, 1131, 1324, 1387
BspFNI CGCG 1 cut(s) 852
BspLI GGNNCC 2 cut(s) 916, 1401
BspMAI CTGCAG 1 cut(s) 900
BspPI GGATC 4 cut(s) 444, 763, 897, 1267
BspT104I TTCGAA 2 cut(s) 270, 1518
BsrDI GCAATG 2 cut(s) 554, 1280
BsrGI TGTACA 1 cut(s) 1546
BsrI ACTGG 5 cut(s) 57, 252, 281, 370, 1018
BssECI CCNNGG 2 cut(s) 934, 1224
BssMI GATC 4 cut(s) 449, 755, 889, 1272
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 934
Bst2UI CCWGG 3 cut(s) 919, 1226, 1536
Bst4CI ACNGT 3 cut(s) 85, 159, 841
Bst6I CTCTTC 2 cut(s) 18, 263
BstACI GRCGYC 1 cut(s) 60
BstAPI GCANNNNNTGC 1 cut(s) 556
BstAUI TGTACA 1 cut(s) 1546
BstBAI YACGTR 1 cut(s) 306
BstBI TTCGAA 2 cut(s) 270, 1518
BstC8I GCNNGC 2 cut(s) 1068, 1363
BstF5I GGATG 2 cut(s) 953, 1054
BstFNI CGCG 1 cut(s) 852
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 4 cut(s) 452, 758, 892, 1275
BstMAI GTCTC 4 cut(s) 260, 409, 1284, 1357
BstMBI GATC 4 cut(s) 449, 755, 889, 1272
BstMWI GCNNNNNNNGC 3 cut(s) 124, 138, 556
BstNI CCWGG 3 cut(s) 919, 1226, 1536
BstNSI RCATGY 1 cut(s) 1264
BstSCI CCNGG 3 cut(s) 917, 1224, 1534
BstSFI CTRYAG 1 cut(s) 896
BstSLI GKGCMC 1 cut(s) 1005
BstUI CGCG 1 cut(s) 852
BstV1I GCAGC 5 cut(s) 337, 882, 1006, 1009, 1177
BstV2I GAAGAC 3 cut(s) 204, 710, 1224
BstX2I RGATCY 2 cut(s) 449, 889
BstYI RGATCY 2 cut(s) 449, 889
Bsu36I CCTNAGG 1 cut(s) 1374
BsuRI GGCC 2 cut(s) 141, 1379
BtgZI GCGATG 1 cut(s) 536
BtsCI GGATG 2 cut(s) 953, 1054
BtsIMutI CAGTG 4 cut(s) 274, 377, 824, 1011
Cac8I GCNNGC 2 cut(s) 1068, 1363
CfoI GCGC 1 cut(s) 550
Cfr13I GGNCC 4 cut(s) 140, 1080, 1400, 1532
CseI GACGC 2 cut(s) 49, 1163
Csp6I GTAC 1 cut(s) 1547
CviAII CATG 3 cut(s) 427, 1171, 1261
CviQI GTAC 1 cut(s) 1547
DpnI GATC 4 cut(s) 451, 757, 891, 1274
DpnII GATC 4 cut(s) 449, 755, 889, 1272
Eam1104I CTCTTC 2 cut(s) 18, 263
EarI CTCTTC 2 cut(s) 18, 263
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 934
Eco147I AGGCCT 1 cut(s) 1379
Eco24I GRGCYC 1 cut(s) 9
Eco47I GGWCC 3 cut(s) 1080, 1400, 1532
Eco53kI GAGCTC 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 704
Eco81I CCTNAGG 1 cut(s) 1374
Eco88I CYCGRG 2 cut(s) 655, 1058
EcoICRI GAGCTC 1 cut(s) 7
EcoO109I RGGNCCY 3 cut(s) 140, 1400, 1532
EcoRI GAATTC 2 cut(s) 441, 1322
EcoRII CCWGG 3 cut(s) 917, 1224, 1534
EcoT14I CCWWGG 1 cut(s) 934
EcoT22I ATGCAT 1 cut(s) 1262
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 934
FaeI CATG 3 cut(s) 430, 1174, 1264
FaqI GGGAC 2 cut(s) 63, 1320
FatI CATG 3 cut(s) 426, 1170, 1260
FauNDI CATATG 1 cut(s) 403
Fnu4HI GCNGC 5 cut(s) 326, 896, 1020, 1023, 1191
FokI GGATG 2 cut(s) 940, 1041
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 5 cut(s) 326, 896, 1020, 1023, 1191
FspBI CTAG 4 cut(s) 38, 353, 935, 1115
GlaI GCGC 1 cut(s) 549
GluI GCNGC 5 cut(s) 326, 896, 1020, 1023, 1191
GsuI CTGGAG 1 cut(s) 298
HaeIII GGCC 2 cut(s) 141, 1379
HapII CCGG 1 cut(s) 347
HgaI GACGC 2 cut(s) 49, 1163
HhaI GCGC 1 cut(s) 550
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 3 cut(s) 430, 1174, 1264
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HincII GTYRAC 1 cut(s) 973
HindII GTYRAC 1 cut(s) 973
HindIII AAGCTT 1 cut(s) 707
HpaII CCGG 1 cut(s) 347
HphI GGTGA 1 cut(s) 1168
Hpy166II GTNNAC 5 cut(s) 340, 973, 1266, 1476, 1547
Hpy188I TCNGA 9 cut(s) 23, 100, 110, 207, 226, 449, 883, 1038, 1570
Hpy188III TCNNGA 5 cut(s) 655, 1058, 1078, 1163, 1203
Hpy8I GTNNAC 5 cut(s) 340, 973, 1266, 1476, 1547
Hpy99I CGWCG 1 cut(s) 883
HpyAV CCTTC 8 cut(s) 105, 159, 352, 408, 578, 754, 854, 1438
HpyCH4III ACNGT 3 cut(s) 85, 159, 841
HpyCH4IV ACGT 1 cut(s) 305
HpyF10VI GCNNNNNNNGC 3 cut(s) 124, 138, 556
HpySE526I ACGT 1 cut(s) 305
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 3 cut(s) 430, 1174, 1264
HspAI GCGC 1 cut(s) 548
Kzo9I GATC 4 cut(s) 449, 755, 889, 1272
LmnI GCTCC 6 cut(s) 4, 123, 349, 427, 914, 1134
Lsp1109I GCAGC 5 cut(s) 337, 882, 1006, 1009, 1177
LweI GCATC 5 cut(s) 141, 168, 1029, 1108, 1303
MaeI CTAG 4 cut(s) 38, 353, 935, 1115
MaeII ACGT 1 cut(s) 305
MaeIII GTNAC 2 cut(s) 468, 822
MalI GATC 4 cut(s) 451, 757, 891, 1274
MboI GATC 4 cut(s) 449, 755, 889, 1272
MboII GAAGA 9 cut(s) 5, 24, 59, 204, 250, 493, 710, 867, 1229
MflI RGATCY 2 cut(s) 449, 889
MhlI GDGCHC 2 cut(s) 9, 1005
MlyI GAGTC 2 cut(s) 551, 830
MmeI TCCRAC 3 cut(s) 217, 519, 861
Mph1103I ATGCAT 1 cut(s) 1262
MroXI GAANNNNTTC 2 cut(s) 28, 730
MseI TTAA 3 cut(s) 296, 629, 780
MslI CAYNNNNRTG 3 cut(s) 333, 829, 1226
MspA1I CMGCKG 2 cut(s) 644, 1190
MspI CCGG 1 cut(s) 347
MspR9I CCNGG 3 cut(s) 919, 1226, 1536
Mva1269I GAATGC 1 cut(s) 1242
MvaI CCWGG 3 cut(s) 919, 1226, 1536
MvnI CGCG 1 cut(s) 852
MwoI GCNNNNNNNGC 3 cut(s) 124, 138, 556
NdeI CATATG 1 cut(s) 403
NdeII GATC 4 cut(s) 449, 755, 889, 1272
NlaIII CATG 3 cut(s) 430, 1174, 1264
NlaIV GGNNCC 2 cut(s) 916, 1401
NmuCI GTSAC 2 cut(s) 468, 822
NsiI ATGCAT 1 cut(s) 1262
NspI RCATGY 1 cut(s) 1264
NspV TTCGAA 2 cut(s) 270, 1518
OliI CACNNNNGTG 1 cut(s) 1226
PaeR7I CTCGAG 1 cut(s) 655
PceI AGGCCT 1 cut(s) 1379
PctI GAATGC 1 cut(s) 1242
PdmI GAANNNNTTC 2 cut(s) 28, 730
PfeI GAWTC 8 cut(s) 24, 166, 610, 950, 963, 1007, 1074, 1159
PflFI GACNNNGTC 1 cut(s) 512
PfoI TCCNGGA 1 cut(s) 1534
PkrI GCNGC 5 cut(s) 327, 897, 1021, 1024, 1192
PleI GAGTC 2 cut(s) 550, 829
PpsI GAGTC 2 cut(s) 550, 829
Ppu21I YACGTR 1 cut(s) 306
PpuMI RGGWCCY 2 cut(s) 1400, 1532
PshBI ATTAAT 1 cut(s) 296
Psp124BI GAGCTC 1 cut(s) 9
Psp5II RGGWCCY 2 cut(s) 1400, 1532
Psp6I CCWGG 3 cut(s) 917, 1224, 1534
PspGI CCWGG 3 cut(s) 917, 1224, 1534
PspN4I GGNNCC 2 cut(s) 916, 1401
PspPI GGNCC 4 cut(s) 140, 1080, 1400, 1532
PspPPI RGGWCCY 2 cut(s) 1400, 1532
PstI CTGCAG 1 cut(s) 900
PsuI RGATCY 2 cut(s) 449, 889
PsyI GACNNNGTC 1 cut(s) 512
PvuII CAGCTG 2 cut(s) 644, 1190
RsaI GTAC 1 cut(s) 1548
RsaNI GTAC 1 cut(s) 1547
RseI CAYNNNNRTG 3 cut(s) 333, 829, 1226
SacI GAGCTC 1 cut(s) 9
SaqAI TTAA 3 cut(s) 296, 629, 780
SatI GCNGC 5 cut(s) 326, 896, 1020, 1023, 1191
Sau3AI GATC 4 cut(s) 449, 755, 889, 1272
Sau96I GGNCC 4 cut(s) 140, 1080, 1400, 1532
SchI GAGTC 2 cut(s) 551, 830
ScrFI CCNGG 3 cut(s) 919, 1226, 1536
SduI GDGCHC 2 cut(s) 9, 1005
SfaNI GCATC 5 cut(s) 141, 168, 1029, 1108, 1303
SfcI CTRYAG 1 cut(s) 896
Sfr274I CTCGAG 1 cut(s) 655
SfuI TTCGAA 2 cut(s) 270, 1518
SinI GGWCC 3 cut(s) 1080, 1400, 1532
SlaI CTCGAG 1 cut(s) 655
SmiMI CAYNNNNRTG 3 cut(s) 333, 829, 1226
SmlI CTYRAG 1 cut(s) 655
SmoI CTYRAG 1 cut(s) 655
SseBI AGGCCT 1 cut(s) 1379
SspMI CTAG 4 cut(s) 38, 353, 935, 1115
SstI GAGCTC 1 cut(s) 9
StuI AGGCCT 1 cut(s) 1379
StyD4I CCNGG 3 cut(s) 917, 1224, 1534
StyI CCWWGG 1 cut(s) 934
TaaI ACNGT 3 cut(s) 85, 159, 841
TaiI ACGT 1 cut(s) 308
TaqI TCGA 5 cut(s) 270, 656, 1072, 1405, 1518
TaqII GACCGA 1 cut(s) 1097
TatI WGTACW 1 cut(s) 1546
TfiI GAWTC 8 cut(s) 24, 166, 610, 950, 963, 1007, 1074, 1159
Tru1I TTAA 3 cut(s) 296, 629, 780
Tru9I TTAA 3 cut(s) 296, 629, 780
TscAI CASTG 4 cut(s) 281, 377, 831, 1018
TseFI GTSAC 2 cut(s) 468, 822
TseI GCWGC 5 cut(s) 325, 895, 1019, 1022, 1190
Tsp45I GTSAC 2 cut(s) 468, 822
TspDTI ATGAA 9 cut(s) 5, 59, 380, 390, 942, 955, 1163, 1330, 1335
TspGWI ACGGA 1 cut(s) 306
TspRI CASTG 4 cut(s) 281, 377, 831, 1018
Tth111I GACNNNGTC 1 cut(s) 512
VpaK11BI GGWCC 3 cut(s) 1080, 1400, 1532
VspI ATTAAT 1 cut(s) 296
XapI RAATTY 4 cut(s) 227, 441, 1322, 1367
XceI RCATGY 1 cut(s) 1264
XhoI CTCGAG 1 cut(s) 655
XmaJI CCTAGG 1 cut(s) 934
XmnI GAANNNNTTC 2 cut(s) 28, 730
XspI CTAG 4 cut(s) 38, 353, 935, 1115
Zsp2I ATGCAT 1 cut(s) 1262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.