FvH4_7g26340

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004647
Physical Location & Seq
Reverse (-)
19925007 .. 19929833
4827 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g26340.t2

Sequence Viewer

Length: 1455 bp
ATGCAAGGCATCAAAGGCCCTACACCTTCTCTACTACATGGGAATCTAGCTGAGATGCAGAAAATCCAACTACTCGAAGCTCAGAAGTCTGCAAATCACTCGGAATTTGTAGCTCACGACTACACTTCCAGCCTCTTCCCCTATTTCGAACACTGGAGAAAGGAATATGGTTTAATCTACACGTATTCAACAGGAATGAGGCAGCATGTATATGTGAACCAACCCGAGCTAGTGAAGGAGATGAACCAGTGTATTAGTTTGGATTTGGGTAAGCCTTCCTATGTAAGCAAGAGACTTGCTCCCATGCTTGGAAATGGCATTCTCAGATCCAATGGTGTTGTTTGGTCACAACAAAGAAAAATTGTTGCACCTGAGTTCTTCGCAGACAAAGTAAAGGCGATGGTAGGATTGATGTTGGAGTCGGCGCAACCATTGCTTAGAAAATGGGGGGAGTGTATTGAAGGACAAGGAGATATTAGAGCAGAGATTCAAGTAGATGAGGATTTAAGGGATTTCTCGGCTGATGTTATCTCTAGGGCTTGTTTTGGGAGTTCTTATTCCAAGGGCAAAGAGATTTTCTCAAAGCTTAGAAGTCTTCAGGAAATCATGTCTCATCAACCCTTCCTCTTTGGATCATTTTCTAGTTTTGGAGGGTTGAAGAAGCAAAAGGAGATAAGTTGTTTAGAGAGAGAGATTGAGTCGTTGATATGGGAAGCTGTGAAAGAACGTGAACGAGAACGGTTAGAGACATCTTCGTCGGAAAAGGATCTATTGCAGACAATGTTAGCCGGAGCCATGAACGACCAAAGCCTAGGCCAAGGCAAGGATTCATCTAAGAAATTTATTATTGACAACTGCAAGAACATTTACTTTGCAGGGCATGAATCCACTGGTGTAGCTGCCTCTTGGTGTTTGATGCTGCTCGCTTTACATCCCGAGTGGCAAGCTCGAATTAGGACTGAGTTGGCGCAAGTTTGCCCCGACGGTCAACTAGATTCTAATTCACTCTCTCAATTAAAGACGCTGACTATGGTGATTCAAGAAGTCATGCGTCTGTACCCACCAGCTGCTTTTGTGTCAAGAGAAGCACTTGAAGACACCCAGGTGGGAAACATAAACATTCCAAAAGGGGTATGCTTGTGGACTTTGATCCCAACATTGCACAGAGACACAGAGACTTGGGGACAAGATGCAAATGATTTCAAACCTGAGAGGTTCACTGATGGTGTCTCTAAGGCTTGCAAGTTTCCTCAAGCCTATATCCCTTTTGGTTTGGGTCCTCGAACTTGTTTAGGCAAAAACTTTGCTATGGTAGAATTGAAGGTTGTCCTCTCCCTCATAATCTCCAAGTTCACTTTCTCTCTATCACCCAAATACAGACATTCTCCTGCTTACAAAATGATTGTAGGTCCTGGAAATGGTGTCCATATTCTCATTCAAAAATCTCATCTGTAG

Protein Analysis

485

Amino Acids

54.47

Weight (kDa)

8.21

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 85 - 504 1.1e-79 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 754
AclWI GGATC 4 cut(s) 321, 640, 774, 1144
AcsI RAATTY 2 cut(s) 104, 839
AcuI CTGAAG 1 cut(s) 581
AfaI GTAC 1 cut(s) 1058
AfiI CCNNNNNNNGG 3 cut(s) 308, 823, 1418
AflIII ACRYGT 1 cut(s) 180
AgsI TTSAA 9 cut(s) 189, 461, 491, 658, 1040, 1094, 1204, 1321, 1439
AjnI CCWGG 2 cut(s) 1101, 1411
AjuI GAANNNNNNNTTGG 2 cut(s) 1340, 1372
AleI CACNNNNGTG 1 cut(s) 1103
AluBI AGCT 9 cut(s) 50, 80, 113, 229, 586, 716, 899, 947, 1067
AluI AGCT 9 cut(s) 50, 80, 113, 229, 586, 716, 899, 947, 1067
Alw26I GTCTC 6 cut(s) 286, 615, 740, 1161, 1169, 1234
AlwI GGATC 4 cut(s) 321, 640, 774, 1144
Ama87I CYCGRG 2 cut(s) 224, 935
AoxI GGCC 2 cut(s) 16, 814
ApeKI GCWGC 4 cut(s) 202, 899, 919, 1067
ApoI RAATTY 2 cut(s) 104, 839
AspA2I CCTAGG 1 cut(s) 811
AspLEI GCGC 2 cut(s) 427, 970
AspS9I GGNCC 3 cut(s) 17, 1277, 1409
AsuHPI GGTGA 2 cut(s) 1045, 1359
AsuII TTCGAA 1 cut(s) 147
AvaI CYCGRG 2 cut(s) 224, 935
AvaII GGWCC 2 cut(s) 1277, 1409
AvrII CCTAGG 1 cut(s) 811
BbsI GAAGAC 2 cut(s) 587, 1101
BbvI GCAGC 4 cut(s) 214, 886, 906, 1054
BccI CCATC 2 cut(s) 394, 1217
BcgI CGANNNNNNTGC 2 cut(s) 81, 115
BciT130I CCWGG 2 cut(s) 1103, 1413
BcoDI GTCTC 6 cut(s) 286, 615, 740, 1161, 1169, 1234
BfaI CTAG 6 cut(s) 47, 230, 534, 642, 812, 992
BfmI CTRYAG 1 cut(s) 1451
BisI GCNGC 4 cut(s) 203, 900, 920, 1068
BlnI CCTAGG 1 cut(s) 811
BlsI GCNGC 4 cut(s) 204, 901, 921, 1069
Bme1390I CCNGG 2 cut(s) 1103, 1413
Bme18I GGWCC 2 cut(s) 1277, 1409
BmeT110I CYCGRG 2 cut(s) 224, 935
BmgT120I GGNCC 3 cut(s) 17, 1277, 1409
BmiI GGNNCC 2 cut(s) 793, 1278
BmrFI CCNGG 2 cut(s) 1103, 1413
BmsI GCATC 4 cut(s) 18, 45, 906, 1180
BpiI GAAGAC 2 cut(s) 587, 1101
BplI GAGNNNNNCTC 4 cut(s) 283, 315, 563, 595
BpmI CTGGAG 1 cut(s) 175
Bpu14I TTCGAA 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 1236
BsaAI YACGTR 1 cut(s) 183
BsaJI CCNNGG 4 cut(s) 561, 811, 817, 1101
Bsc4I CCNNNNNNNGG 3 cut(s) 308, 823, 1418
Bse1I ACTGG 3 cut(s) 158, 247, 895
Bse3DI GCAATG 2 cut(s) 431, 1157
BseBI CCWGG 2 cut(s) 1103, 1413
BseDI CCNNGG 4 cut(s) 561, 811, 817, 1101
BseGI GGATG 1 cut(s) 931
BseLI CCNNNNNNNGG 3 cut(s) 308, 823, 1418
BseMI GCAATG 2 cut(s) 431, 1157
BseMII CTCAG 6 cut(s) 42, 95, 337, 363, 951, 1200
BseNI ACTGG 3 cut(s) 158, 247, 895
BseXI GCAGC 4 cut(s) 214, 886, 906, 1054
BshFI GGCC 2 cut(s) 18, 816
BsiHKCI CYCGRG 2 cut(s) 224, 935
BsiSI CCGG 1 cut(s) 789
BslFI GGGAC 1 cut(s) 1197
BslI CCNNNNNNNGG 3 cut(s) 308, 823, 1418
BsmAI GTCTC 6 cut(s) 286, 615, 740, 1161, 1169, 1234
BsmFI GGGAC 1 cut(s) 1197
BsmI GAATGC 1 cut(s) 318
BsnI GGCC 2 cut(s) 18, 816
BsoBI CYCGRG 2 cut(s) 224, 935
Bsp119I TTCGAA 1 cut(s) 147
Bsp143I GATC 4 cut(s) 326, 632, 766, 1149
BspANI GGCC 2 cut(s) 18, 816
BspCNI CTCAG 6 cut(s) 43, 94, 336, 364, 952, 1201
BspLI GGNNCC 2 cut(s) 793, 1278
BspPI GGATC 4 cut(s) 321, 640, 774, 1144
BspT104I TTCGAA 1 cut(s) 147
BsrDI GCAATG 2 cut(s) 431, 1157
BsrI ACTGG 3 cut(s) 158, 247, 895
BssECI CCNNGG 4 cut(s) 561, 811, 817, 1101
BssMI GATC 4 cut(s) 326, 632, 766, 1149
BssT1I CCWWGG 3 cut(s) 561, 811, 817
Bst2UI CCWGG 2 cut(s) 1103, 1413
Bst4CI ACNGT 2 cut(s) 741, 986
Bst6I CTCTTC 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 433
BstBAI YACGTR 1 cut(s) 183
BstBI TTCGAA 1 cut(s) 147
BstC8I GCNNGC 3 cut(s) 924, 945, 1240
BstF5I GGATG 1 cut(s) 931
BstHHI GCGC 2 cut(s) 427, 970
BstKTI GATC 4 cut(s) 329, 635, 769, 1152
BstMAI GTCTC 6 cut(s) 286, 615, 740, 1161, 1169, 1234
BstMBI GATC 4 cut(s) 326, 632, 766, 1149
BstMWI GCNNNNNNNGC 2 cut(s) 15, 433
BstNI CCWGG 2 cut(s) 1103, 1413
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 2 cut(s) 1101, 1411
BstSFI CTRYAG 1 cut(s) 1451
BstV1I GCAGC 4 cut(s) 214, 886, 906, 1054
BstV2I GAAGAC 2 cut(s) 587, 1101
BstX2I RGATCY 2 cut(s) 326, 766
BstYI RGATCY 2 cut(s) 326, 766
BsuRI GGCC 2 cut(s) 18, 816
BtgZI GCGATG 1 cut(s) 413
BtsCI GGATG 1 cut(s) 931
BtsIMutI CAGTG 4 cut(s) 151, 254, 888, 1218
Cac8I GCNNGC 3 cut(s) 924, 945, 1240
CfoI GCGC 2 cut(s) 427, 970
Cfr13I GGNCC 3 cut(s) 17, 1277, 1409
CseI GACGC 2 cut(s) 1030, 1040
Csp6I GTAC 1 cut(s) 1057
CviAII CATG 7 cut(s) 38, 206, 304, 607, 796, 881, 1048
CviQI GTAC 1 cut(s) 1057
DpnI GATC 4 cut(s) 328, 634, 768, 1151
DpnII GATC 4 cut(s) 326, 632, 766, 1149
DrdI GACNNNNNNGTC 1 cut(s) 754
DseDI GACNNNNNNGTC 1 cut(s) 754
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Eco130I CCWWGG 3 cut(s) 561, 811, 817
Eco47I GGWCC 2 cut(s) 1277, 1409
Eco57I CTGAAG 1 cut(s) 581
Eco88I CYCGRG 2 cut(s) 224, 935
EcoO109I RGGNCCY 3 cut(s) 17, 1277, 1409
EcoRII CCWGG 2 cut(s) 1101, 1411
EcoT14I CCWWGG 3 cut(s) 561, 811, 817
ErhI CCWWGG 3 cut(s) 561, 811, 817
FaeI CATG 7 cut(s) 41, 209, 307, 610, 799, 884, 1051
FaqI GGGAC 1 cut(s) 1197
FatI CATG 7 cut(s) 37, 205, 303, 606, 795, 880, 1047
Fnu4HI GCNGC 4 cut(s) 203, 900, 920, 1068
FokI GGATG 1 cut(s) 918
Fsp4HI GCNGC 4 cut(s) 203, 900, 920, 1068
FspBI CTAG 6 cut(s) 47, 230, 534, 642, 812, 992
GlaI GCGC 2 cut(s) 426, 969
GluI GCNGC 4 cut(s) 203, 900, 920, 1068
GsuI CTGGAG 1 cut(s) 175
HaeIII GGCC 2 cut(s) 18, 816
HapII CCGG 1 cut(s) 789
HgaI GACGC 2 cut(s) 1030, 1040
HhaI GCGC 2 cut(s) 427, 970
Hin1II CATG 7 cut(s) 41, 209, 307, 610, 799, 884, 1051
Hin6I GCGC 2 cut(s) 425, 968
HinP1I GCGC 2 cut(s) 425, 968
HincII GTYRAC 1 cut(s) 989
HindII GTYRAC 1 cut(s) 989
HindIII AAGCTT 1 cut(s) 584
HinfI GANTC 8 cut(s) 43, 419, 487, 698, 827, 884, 995, 1036
HpaII CCGG 1 cut(s) 789
HphI GGTGA 2 cut(s) 1045, 1359
Hpy166II GTNNAC 6 cut(s) 217, 731, 989, 1143, 1218, 1353
Hpy188I TCNGA 4 cut(s) 84, 103, 326, 760
Hpy188III TCNNGA 5 cut(s) 116, 599, 935, 1040, 1080
Hpy8I GTNNAC 6 cut(s) 217, 731, 989, 1143, 1218, 1353
Hpy99I CGWCG 2 cut(s) 760, 986
HpyAV CCTTC 6 cut(s) 36, 229, 285, 455, 631, 1315
HpyCH4III ACNGT 2 cut(s) 741, 986
HpyCH4IV ACGT 2 cut(s) 182, 727
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 433
HpySE526I ACGT 2 cut(s) 182, 727
Hsp92II CATG 7 cut(s) 41, 209, 307, 610, 799, 884, 1051
HspAI GCGC 2 cut(s) 425, 968
Kzo9I GATC 4 cut(s) 326, 632, 766, 1149
LmnI GCTCC 2 cut(s) 304, 791
Lsp1109I GCAGC 4 cut(s) 214, 886, 906, 1054
LweI GCATC 4 cut(s) 18, 45, 906, 1180
MaeI CTAG 6 cut(s) 47, 230, 534, 642, 812, 992
MaeII ACGT 2 cut(s) 182, 727
MaeIII GTNAC 1 cut(s) 345
MalI GATC 4 cut(s) 328, 634, 768, 1151
MboI GATC 4 cut(s) 326, 632, 766, 1149
MboII GAAGA 6 cut(s) 127, 370, 587, 670, 744, 1106
MflI RGATCY 2 cut(s) 326, 766
MluCI AATT 7 cut(s) 104, 360, 839, 951, 1000, 1013, 1316
MlyI GAGTC 2 cut(s) 428, 707
MmeI TCCRAC 3 cut(s) 91, 396, 738
MseI TTAA 3 cut(s) 173, 506, 1016
MslI CAYNNNNRTG 2 cut(s) 210, 1103
MspA1I CMGCKG 1 cut(s) 1067
MspI CCGG 1 cut(s) 789
MspR9I CCNGG 2 cut(s) 1103, 1413
Mva1269I GAATGC 1 cut(s) 318
MvaI CCWGG 2 cut(s) 1103, 1413
MwoI GCNNNNNNNGC 2 cut(s) 15, 433
NdeII GATC 4 cut(s) 326, 632, 766, 1149
NlaIII CATG 7 cut(s) 41, 209, 307, 610, 799, 884, 1051
NlaIV GGNNCC 2 cut(s) 793, 1278
NmeAIII GCCGAG 1 cut(s) 497
NmuCI GTSAC 1 cut(s) 345
NspI RCATGY 1 cut(s) 209
NspV TTCGAA 1 cut(s) 147
OliI CACNNNNGTG 1 cut(s) 1103
PctI GAATGC 1 cut(s) 318
PfeI GAWTC 6 cut(s) 43, 487, 827, 884, 995, 1036
PfoI TCCNGGA 1 cut(s) 1411
PkrI GCNGC 4 cut(s) 204, 901, 921, 1069
PleI GAGTC 2 cut(s) 427, 706
PpsI GAGTC 2 cut(s) 427, 706
Ppu21I YACGTR 1 cut(s) 183
PpuMI RGGWCCY 2 cut(s) 1277, 1409
Psp5II RGGWCCY 2 cut(s) 1277, 1409
Psp6I CCWGG 2 cut(s) 1101, 1411
PspGI CCWGG 2 cut(s) 1101, 1411
PspN4I GGNNCC 2 cut(s) 793, 1278
PspPI GGNCC 3 cut(s) 17, 1277, 1409
PspPPI RGGWCCY 2 cut(s) 1277, 1409
PsuI RGATCY 2 cut(s) 326, 766
PvuII CAGCTG 1 cut(s) 1067
RsaI GTAC 1 cut(s) 1058
RsaNI GTAC 1 cut(s) 1057
RseI CAYNNNNRTG 2 cut(s) 210, 1103
SaqAI TTAA 3 cut(s) 173, 506, 1016
SatI GCNGC 4 cut(s) 203, 900, 920, 1068
Sau3AI GATC 4 cut(s) 326, 632, 766, 1149
Sau96I GGNCC 3 cut(s) 17, 1277, 1409
SchI GAGTC 2 cut(s) 428, 707
ScrFI CCNGG 2 cut(s) 1103, 1413
SfaNI GCATC 4 cut(s) 18, 45, 906, 1180
SfcI CTRYAG 1 cut(s) 1451
SfuI TTCGAA 1 cut(s) 147
SinI GGWCC 2 cut(s) 1277, 1409
SmiMI CAYNNNNRTG 2 cut(s) 210, 1103
SmlI CTYRAG 1 cut(s) 1251
SmoI CTYRAG 1 cut(s) 1251
Sse9I AATT 7 cut(s) 104, 360, 839, 951, 1000, 1013, 1316
SspMI CTAG 6 cut(s) 47, 230, 534, 642, 812, 992
StyD4I CCNGG 2 cut(s) 1101, 1411
StyI CCWWGG 3 cut(s) 561, 811, 817
TaaI ACNGT 2 cut(s) 741, 986
TaiI ACGT 2 cut(s) 185, 730
TaqI TCGA 4 cut(s) 75, 147, 949, 1282
TasI AATT 7 cut(s) 104, 360, 839, 951, 1000, 1013, 1316
TfiI GAWTC 6 cut(s) 43, 487, 827, 884, 995, 1036
Tru1I TTAA 3 cut(s) 173, 506, 1016
Tru9I TTAA 3 cut(s) 173, 506, 1016
TscAI CASTG 4 cut(s) 158, 254, 895, 1225
TseFI GTSAC 1 cut(s) 345
TseI GCWGC 4 cut(s) 202, 899, 919, 1067
Tsp45I GTSAC 1 cut(s) 345
TspDTI ATGAA 4 cut(s) 257, 812, 819, 897
TspRI CASTG 4 cut(s) 158, 254, 895, 1225
VpaK11BI GGWCC 2 cut(s) 1277, 1409
XapI RAATTY 2 cut(s) 104, 839
XceI RCATGY 1 cut(s) 209
XmaJI CCTAGG 1 cut(s) 811
XspI CTAG 6 cut(s) 47, 230, 534, 642, 812, 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.