AT5G27300

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004971
Physical Location & Seq
Forward (+)
9620810 .. 9625136
4327 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27300.1

Sequence Viewer

Length: 1728 bp
ATGGCTGCAACTAGGTCTACATTTCTAGGCTCCATCTTCAGAACCGTTAAAGCACGAGTTTTGATCCCACCCATCACAAGACTCTCTCTCCCAAACCCTAATTTCACTTCCTCTCGCTTCCACACTTCTTCTTCTCTGCCTCAGTCTCAAATCGAAGGTTCCATTTCGAGTTCTCTTCTGCAAGACCTGAGCATCATCCATGGTGTGATTGCGATTGCTTCCACTAGTCAAGGTCGATTAGCATCTGTTTCCATGTGTACACAATACTCAACTTCAGTCCCAACACGTTCACTTAGAAGGAGAATCAGCAGTAGAAAGAAATCGAGTACAAAACCAATCCTTAATGAATCAAAGTTTCAAGAAACGATATCAAAGCTTCCACCAAGATTCACACCTGAAGAACTAGCTGATGCTATAACTCTTGAAGAAGACCCGTTTCTGTGTTTCCATCTCTTTAACTGGGCATCGCAACAGCCGAGGTTTACGCATGAGAATTGCTCTTACCATATCGCGATAAGGAAGCTCGGTGCTGCGAAATCTGGGAAGTTGATACGTGCTGTGAATATATTTAGACATATGGTGAATAGTAGGAACTTGGAATGTAGACCAACGATGAGAACGTATCATATTCTCTTCAAAGCATTGTTGGGTAGAGGTAACAACTCTTTCATAAACCATCTGTATATGGAGACAGTAAGATCTTTGTTTCGACAAATGGTGGATAGTGGTATTGAACCAGACGTATTTGCTTTGAACTGTTTGGTTAAAGGTAGGACCATCAATACTAGAGAATTGCTTAGTGAGATGAAAGGAAAAGGCTTTGTTCCTAATGGGAAATCTTATAACTCTCTGGTTAATGCTTTTGCGCTTAGTGGTGAGATTGATGATGCGGTGAAATGTTTGTGGGAGATGATTGAGAATGGTCGTGTGGTTGATTTTATTAGCTATAGAACACTTGTTGATGAGAGTTGTAGGAAAGGGAAGTATGATGAAGCGACGAGATTGTTGGAGATGTTGCGAGAGAAACAGCTTGTGGATATAGATTCTGATGATAAGCTTAAGATGTACCAAATGGTGATTCTTGTTCTTCTTTTCTCCTCAATGTTGCCTTCAGTTTGCGATGAATCAAGATACATGATAGTACGTAATGTGCCAGCTTTGGGTTGTGGTGATGATCTCATGAGATTATTCATGACTTATGGAGAAGTTGAAGAATGTAAACCTATGGATGCAGAAGACTGTGCGGAGTTCACGGATGTCTACTGGATCAAGTTTCGTCTCATCACTAATGCTAGGAAGTTGGATGAATCAAGTTTTTTGGGAAATCGGCTCCAAATCTCATATGCTCCTGAATACGAGAGCGTCAGTGACACAAAGGAGAAGTTAGAAACTAGAAGGAAAGAAGTGCTTGCAAGACTGAACCCCCATAAAGCAAAGAGCACCTCCCAAGTTACAAAATTGGCTGGACCAGCTTTGACCCAAACCGACAATTTTTCCCCTCGGCGGAGAGAGATGGATTACCAATTCCATAGAGGAAATGCTCCTGTTACTCGAGTTTCATCATATCAGGAGTATTTTGCGTCATCTTCAATGAATCAGATGGTTAAAACTGTGAGGGAGAAACTCAATAAGATTGAAGAAAGTGGTAACCAAAAGAGGTTACAACCAAGCAGCCAAACACAACCTGACTTCAAGAGAACCCGAGTCGATAACCGAAGAAGAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

575

Amino Acids

65.71

Weight (kDa)

9.48

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 843
AccI GTMKAC 3 cut(s) 17, 604, 1260
AccII CGCG 1 cut(s) 512
AciI CCGC 3 cut(s) 890, 1244, 1504
AclWI GGATC 2 cut(s) 58, 1274
AcuI CTGAAG 4 cut(s) 22, 258, 417, 1095
AfaI GTAC 4 cut(s) 259, 328, 1067, 1143
AfiI CCNNNNNNNGG 2 cut(s) 1160, 1503
AflII CTTAAG 1 cut(s) 1058
AflIII ACRYGT 1 cut(s) 284
AgsI TTSAA 9 cut(s) 359, 425, 637, 734, 754, 1211, 1590, 1637, 1693
AhlI ACTAGT 1 cut(s) 224
AjuI GAANNNNNNNTTGG 2 cut(s) 1062, 1094
AluBI AGCT 8 cut(s) 376, 407, 523, 945, 1030, 1057, 1157, 1472
AluI AGCT 8 cut(s) 376, 407, 523, 945, 1030, 1057, 1157, 1472
Alw21I GWGCWC 1 cut(s) 1442
Alw26I GTCTC 3 cut(s) 150, 683, 1283
AlwI GGATC 2 cut(s) 58, 1274
Ama87I CYCGRG 2 cut(s) 1551, 1701
ApeKI GCWGC 3 cut(s) 5, 530, 1671
AspLEI GCGC 1 cut(s) 868
AspS9I GGNCC 2 cut(s) 774, 1466
AsuHPI GGTGA 5 cut(s) 592, 887, 904, 1087, 1181
AvaI CYCGRG 2 cut(s) 1551, 1701
AvaII GGWCC 2 cut(s) 774, 1466
BauI CACGAG 1 cut(s) 54
BbsI GAAGAC 2 cut(s) 435, 1242
Bbv12I GWGCWC 1 cut(s) 1442
BbvI GCAGC 2 cut(s) 517, 1683
BccI CCATC 7 cut(s) 41, 80, 456, 684, 785, 1507, 1594
BcoDI GTCTC 3 cut(s) 150, 683, 1283
BcuI ACTAGT 1 cut(s) 224
BfaI CTAG 7 cut(s) 12, 26, 225, 404, 786, 1293, 1392
BfmI CTRYAG 1 cut(s) 946
BfrI CTTAAG 1 cut(s) 1058
BglII AGATCT 1 cut(s) 698
BisI GCNGC 3 cut(s) 6, 531, 1672
BlsI GCNGC 3 cut(s) 7, 532, 1673
Bme18I GGWCC 2 cut(s) 774, 1466
BmeT110I CYCGRG 2 cut(s) 1551, 1701
BmgT120I GGNCC 2 cut(s) 774, 1466
BmiI GGNNCC 3 cut(s) 31, 160, 1331
BmrI ACTGGG 1 cut(s) 469
BmsI GCATC 6 cut(s) 201, 251, 400, 473, 877, 1219
BmuI ACTGGG 1 cut(s) 469
BpiI GAAGAC 2 cut(s) 435, 1242
BplI GAGNNNNNCTC 2 cut(s) 482, 514
Bpu10I CCTNAGC 1 cut(s) 188
BsaAI YACGTR 2 cut(s) 554, 1145
BsaBI GATNNNNATC 1 cut(s) 241
BsaJI CCNNGG 3 cut(s) 199, 476, 1499
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 2 cut(s) 1160, 1503
Bse1I ACTGG 2 cut(s) 464, 1268
Bse8I GATNNNNATC 1 cut(s) 241
BseDI CCNNGG 3 cut(s) 199, 476, 1499
BseGI GGATG 4 cut(s) 195, 1234, 1261, 1309
BseJI GATNNNNATC 1 cut(s) 241
BseLI CCNNNNNNNGG 2 cut(s) 1160, 1503
BseMII CTCAG 2 cut(s) 155, 179
BseNI ACTGG 2 cut(s) 464, 1268
BseRI GAGGAG 1 cut(s) 1087
BseXI GCAGC 2 cut(s) 517, 1683
Bsh1236I CGCG 1 cut(s) 512
BsiHKAI GWGCWC 1 cut(s) 1442
BsiHKCI CYCGRG 2 cut(s) 1551, 1701
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 2 cut(s) 1160, 1503
BsmAI GTCTC 3 cut(s) 150, 683, 1283
BsmBI CGTCTC 1 cut(s) 1283
BsmFI GGGAC 1 cut(s) 263
BsoBI CYCGRG 2 cut(s) 1551, 1701
Bsp1286I GDGCHC 1 cut(s) 1442
Bsp1407I TGTACA 1 cut(s) 257
Bsp143I GATC 4 cut(s) 63, 698, 1174, 1266
Bsp19I CCATGG 1 cut(s) 199
Bsp68I TCGCGA 1 cut(s) 512
BspACI CCGC 3 cut(s) 890, 1244, 1504
BspCNI CTCAG 2 cut(s) 154, 180
BspFNI CGCG 1 cut(s) 512
BspHI TCATGA 2 cut(s) 1179, 1191
BspLI GGNNCC 3 cut(s) 31, 160, 1331
BspPI GGATC 2 cut(s) 58, 1274
BspTI CTTAAG 1 cut(s) 1058
BsrGI TGTACA 1 cut(s) 257
BsrI ACTGG 2 cut(s) 464, 1268
BssECI CCNNGG 3 cut(s) 199, 476, 1499
BssMI GATC 4 cut(s) 63, 698, 1174, 1266
BssSI CACGAG 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 199
Bst2BI CACGAG 1 cut(s) 54
Bst4CI ACNGT 5 cut(s) 46, 694, 758, 1241, 1612
Bst6I CTCTTC 2 cut(s) 180, 638
BstAFI CTTAAG 1 cut(s) 1058
BstAUI TGTACA 1 cut(s) 257
BstBAI YACGTR 2 cut(s) 554, 1145
BstC8I GCNNGC 2 cut(s) 1155, 1410
BstDEI CTNAG 5 cut(s) 141, 188, 293, 797, 869
BstDSI CCRYGG 1 cut(s) 199
BstEII GGTNACC 1 cut(s) 1646
BstF5I GGATG 4 cut(s) 195, 1234, 1261, 1309
BstFNI CGCG 1 cut(s) 512
BstHHI GCGC 1 cut(s) 868
BstKTI GATC 4 cut(s) 66, 701, 1177, 1269
BstMAI GTCTC 3 cut(s) 150, 683, 1283
BstMBI GATC 4 cut(s) 63, 698, 1174, 1266
BstMWI GCNNNNNNNGC 1 cut(s) 1469
BstPI GGTNACC 1 cut(s) 1646
BstSFI CTRYAG 1 cut(s) 946
BstSNI TACGTA 1 cut(s) 1145
BstUI CGCG 1 cut(s) 512
BstV1I GCAGC 2 cut(s) 517, 1683
BstV2I GAAGAC 2 cut(s) 435, 1242
BstX2I RGATCY 1 cut(s) 698
BstYI RGATCY 1 cut(s) 698
BtgI CCRYGG 1 cut(s) 199
BtgZI GCGATG 2 cut(s) 450, 1134
BtsCI GGATG 4 cut(s) 195, 1234, 1261, 1309
BtsIMutI CAGTG 1 cut(s) 1372
BtuMI TCGCGA 1 cut(s) 512
Cac8I GCNNGC 2 cut(s) 1155, 1410
CciI TCATGA 2 cut(s) 1179, 1191
CfoI GCGC 1 cut(s) 868
Cfr13I GGNCC 2 cut(s) 774, 1466
CseI GACGC 2 cut(s) 1351, 1569
Csp6I GTAC 4 cut(s) 258, 327, 1066, 1142
CviAII CATG 6 cut(s) 200, 253, 488, 1135, 1180, 1192
CviQI GTAC 4 cut(s) 258, 327, 1066, 1142
DdeI CTNAG 5 cut(s) 141, 188, 293, 797, 869
DpnI GATC 4 cut(s) 65, 700, 1176, 1268
DpnII GATC 4 cut(s) 63, 698, 1174, 1266
Eam1104I CTCTTC 2 cut(s) 180, 638
EarI CTCTTC 2 cut(s) 180, 638
EciI GGCGGA 1 cut(s) 1519
Eco105I TACGTA 1 cut(s) 1145
Eco130I CCWWGG 1 cut(s) 199
Eco32I GATATC 1 cut(s) 369
Eco47I GGWCC 2 cut(s) 774, 1466
Eco57I CTGAAG 4 cut(s) 22, 258, 417, 1095
Eco88I CYCGRG 2 cut(s) 1551, 1701
Eco91I GGTNACC 1 cut(s) 1646
EcoO65I GGTNACC 1 cut(s) 1646
EcoRV GATATC 1 cut(s) 369
EcoT14I CCWWGG 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 199
Esp3I CGTCTC 1 cut(s) 1283
FaeI CATG 6 cut(s) 203, 256, 491, 1138, 1183, 1195
FalI AAGNNNNNCTT 2 cut(s) 1392, 1424
FaqI GGGAC 1 cut(s) 263
FatI CATG 6 cut(s) 199, 252, 487, 1134, 1179, 1191
FauNDI CATATG 2 cut(s) 576, 1342
FblI GTMKAC 3 cut(s) 17, 604, 1260
Fnu4HI GCNGC 3 cut(s) 6, 531, 1672
FokI GGATG 4 cut(s) 182, 1241, 1268, 1316
Fsp4HI GCNGC 3 cut(s) 6, 531, 1672
FspBI CTAG 7 cut(s) 12, 26, 225, 404, 786, 1293, 1392
GlaI GCGC 1 cut(s) 867
GluI GCNGC 3 cut(s) 6, 531, 1672
HgaI GACGC 2 cut(s) 1351, 1569
HhaI GCGC 1 cut(s) 868
Hin1II CATG 6 cut(s) 203, 256, 491, 1138, 1183, 1195
Hin6I GCGC 1 cut(s) 866
HinP1I GCGC 1 cut(s) 866
HindIII AAGCTT 2 cut(s) 374, 1055
HphI GGTGA 5 cut(s) 592, 887, 904, 1087, 1181
Hpy166II GTNNAC 9 cut(s) 18, 258, 260, 290, 483, 605, 1220, 1251, 1261
Hpy188I TCNGA 3 cut(s) 41, 1048, 1599
Hpy188III TCNNGA 9 cut(s) 359, 422, 511, 1128, 1180, 1192, 1349, 1568, 1693
Hpy8I GTNNAC 9 cut(s) 18, 258, 260, 290, 483, 605, 1220, 1251, 1261
Hpy99I CGWCG 1 cut(s) 1000
HpyAV CCTTC 4 cut(s) 149, 291, 1119, 1389
HpyCH4III ACNGT 5 cut(s) 46, 694, 758, 1241, 1612
HpyCH4IV ACGT 5 cut(s) 286, 553, 620, 741, 1144
HpyCH4V TGCA 4 cut(s) 8, 181, 1232, 1412
HpyF10VI GCNNNNNNNGC 1 cut(s) 1469
HpyF3I CTNAG 5 cut(s) 141, 188, 293, 797, 869
HpySE526I ACGT 5 cut(s) 286, 553, 620, 741, 1144
Hsp92II CATG 6 cut(s) 203, 256, 491, 1138, 1183, 1195
HspAI GCGC 1 cut(s) 866
Kzo9I GATC 4 cut(s) 63, 698, 1174, 1266
LmnI GCTCC 4 cut(s) 35, 1335, 1351, 1546
Lsp1109I GCAGC 2 cut(s) 517, 1683
LweI GCATC 6 cut(s) 201, 251, 400, 473, 877, 1219
MaeI CTAG 7 cut(s) 12, 26, 225, 404, 786, 1293, 1392
MaeII ACGT 5 cut(s) 286, 553, 620, 741, 1144
MaeIII GTNAC 6 cut(s) 656, 1367, 1450, 1546, 1646, 1659
MalI GATC 4 cut(s) 65, 700, 1176, 1268
MboI GATC 4 cut(s) 63, 698, 1174, 1266
MflI RGATCY 1 cut(s) 698
MhlI GDGCHC 1 cut(s) 1442
MluCI AATT 6 cut(s) 100, 493, 791, 1457, 1489, 1523
MlyI GAGTC 2 cut(s) 75, 1713
MmeI TCCRAC 2 cut(s) 987, 1281
MseI TTAA 7 cut(s) 48, 342, 456, 765, 855, 1059, 1605
MspCI CTTAAG 1 cut(s) 1058
MvnI CGCG 1 cut(s) 512
MwoI GCNNNNNNNGC 1 cut(s) 1469
NcoI CCATGG 1 cut(s) 199
NdeI CATATG 2 cut(s) 576, 1342
NdeII GATC 4 cut(s) 63, 698, 1174, 1266
NlaIII CATG 6 cut(s) 203, 256, 491, 1138, 1183, 1195
NlaIV GGNNCC 3 cut(s) 31, 160, 1331
NmeAIII GCCGAG 2 cut(s) 501, 1480
NmuCI GTSAC 1 cut(s) 1367
NruI TCGCGA 1 cut(s) 512
PaeR7I CTCGAG 1 cut(s) 1551
PagI TCATGA 2 cut(s) 1179, 1191
PcsI WCGNNNNNNNCGW 2 cut(s) 473, 617
PfeI GAWTC 8 cut(s) 303, 347, 387, 1043, 1078, 1124, 1307, 1594
PkrI GCNGC 3 cut(s) 7, 532, 1673
PleI GAGTC 2 cut(s) 75, 1712
PpsI GAGTC 2 cut(s) 75, 1712
Ppu21I YACGTR 2 cut(s) 554, 1145
PsiI TTATAA 1 cut(s) 843
PspEI GGTNACC 1 cut(s) 1646
PspN4I GGNNCC 3 cut(s) 31, 160, 1331
PspPI GGNCC 2 cut(s) 774, 1466
PspXI VCTCGAGB 1 cut(s) 1551
PsuI RGATCY 1 cut(s) 698
RruI TCGCGA 1 cut(s) 512
RsaI GTAC 4 cut(s) 259, 328, 1067, 1143
RsaNI GTAC 4 cut(s) 258, 327, 1066, 1142
SaqAI TTAA 7 cut(s) 48, 342, 456, 765, 855, 1059, 1605
SatI GCNGC 3 cut(s) 6, 531, 1672
Sau3AI GATC 4 cut(s) 63, 698, 1174, 1266
Sau96I GGNCC 2 cut(s) 774, 1466
SchI GAGTC 2 cut(s) 75, 1713
SduI GDGCHC 1 cut(s) 1442
SfaNI GCATC 6 cut(s) 201, 251, 400, 473, 877, 1219
SfcI CTRYAG 1 cut(s) 946
Sfr274I CTCGAG 1 cut(s) 1551
SinI GGWCC 2 cut(s) 774, 1466
SlaI CTCGAG 1 cut(s) 1551
SmlI CTYRAG 2 cut(s) 1058, 1551
SmoI CTYRAG 2 cut(s) 1058, 1551
SnaBI TACGTA 1 cut(s) 1145
SpeI ACTAGT 1 cut(s) 224
Sse9I AATT 6 cut(s) 100, 493, 791, 1457, 1489, 1523
SsiI CCGC 3 cut(s) 890, 1244, 1504
SspMI CTAG 7 cut(s) 12, 26, 225, 404, 786, 1293, 1392
StyI CCWWGG 1 cut(s) 199
TaaI ACNGT 5 cut(s) 46, 694, 758, 1241, 1612
TaiI ACGT 5 cut(s) 289, 556, 623, 744, 1147
TaqI TCGA 7 cut(s) 153, 167, 235, 323, 709, 1552, 1707
TasI AATT 6 cut(s) 100, 493, 791, 1457, 1489, 1523
TatI WGTACW 2 cut(s) 257, 326
TfiI GAWTC 8 cut(s) 303, 347, 387, 1043, 1078, 1124, 1307, 1594
Tru1I TTAA 7 cut(s) 48, 342, 456, 765, 855, 1059, 1605
Tru9I TTAA 7 cut(s) 48, 342, 456, 765, 855, 1059, 1605
TscAI CASTG 1 cut(s) 1372
TseFI GTSAC 1 cut(s) 1367
TseI GCWGC 3 cut(s) 5, 530, 1671
Tsp45I GTSAC 1 cut(s) 1367
TspDTI ATGAA 9 cut(s) 360, 658, 821, 1005, 1137, 1180, 1320, 1548, 1607
TspGWI ACGGA 1 cut(s) 1268
TspRI CASTG 1 cut(s) 1372
Vha464I CTTAAG 1 cut(s) 1058
VpaK11BI GGWCC 2 cut(s) 774, 1466
XhoI CTCGAG 1 cut(s) 1551
XmiI GTMKAC 3 cut(s) 17, 604, 1260
XspI CTAG 7 cut(s) 12, 26, 225, 404, 786, 1293, 1392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.