AT5G27690

WAS WASL-interacting protein family member 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011694
Physical Location & Seq
Forward (+)
9803647 .. 9805266
1620 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27690.1

Sequence Viewer

Length: 1059 bp
ATGGATTTAGGAACAGAGATAAAATCAGACACAAGACAAGAAGAACAGAGACATGTTTTCGAAGATTACCCTGAACCTCTTAGATACACCACATGGGTTTTGCGAGTTTCTATCCATTGTGAAGGATGCAAGAGAAAAATCAAGAAAATATTGAGCAAAATCGATGGTGTATATACAACAAATATCGACGTGAAGCAACAAAAAGTAACGGTGATCGGAAACGTAGAGCCGGAGATTTTAATCAAGAAGATCATGAAAGCCGGTAGACACGCCGAGCTATGGCCAACGTCAATGGAGAACAACATTAACAACGATTGTAACTATCAGAAGAAACCAAAGAAAGATAACGAAGAAACTAGTGGAGATGAAGACGACGACGAAAACAACAACAACAACAACGGCGGAGGTGACGTCGGTGGTGGAGGTGGTGGTGGAGGAGGAAACTTTGATCAGGTTAAACAAGTTGTTACGTTTGTAAACGGTCAACTTCAACCACAAGGAGATGGAGCTCCGAAGAAGAAGAAGAAAAAGAAAAAGAAGAAGAAAAGCTTAGGGAACACGACGGTGGTAATGGAAGGAGGCGGCGGCGGCGGTGGCGGTGGTGGTCCTCCGCAAAACGATGGACCGCCAGAGACAGTGATATACTCAGCTCCACAAGATCATCACATTATACACGGACCACCTCCTCATCTACATCATCATCAGCAGCAGAATCATCCGTATCCAACGGTTCACAGTCCTCCACGTCATCATCCACAACAAATGTACGGGCCGGGTCCGGGACTGCCTCCTCCTTCGTTTTATCATACTCAGCCTCAAACGGCGCCGTCTTATACGGTGAGTTACAACACGGTGCATGGGCCAATTAATAACGGTGGGAATGAGACGGCGTCGTATTACACGGCTCCTCCTTCTCATCCGACGTCATATTATTCTTATGAGTACGTGGACACTGGCTACGAATCTCCACCGCCCGAATTTTATTCGTATAGATCTCAACCGTCGGAATCGTTTGAGTCACTAAGCGAAGGCAATCCAAACTCTTGTTGCGTTATGTGA

Protein Analysis

352

Amino Acids

38.7

Weight (kDa)

6.38

Isoelectric Point (pI)

54.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 34 - 90 2.5e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011694)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27690
fragaria_vesca FvH4_2g25240 FvH4_3g05420
malus_domestica MD03G1282300.v1.1 MD11G1304000.v1.1
prunus_persica Prupe.8G257200_v2.0.a1
pyrus_communis pycom03g22310 pycom11g26560
rosa_chinensis RchiOBHm_Chr6g0293381
rosa_laevigata RLG00000011951
rosa_multiflora Rmu_sc0006269.1_g000028
rosa_roxburghii Rroxscaffold_7G00174210
rosa_rugosa Rorug06G0235000 Rorug06G0235100
rosa_samantha Rh6AG347900 Rh6BG355500 Rh6CG361600 Rh6DG348700
rosa_wichuraiana Rw6G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 414, 926
AccB1I GGYRCC 1 cut(s) 823
AccI GTMKAC 1 cut(s) 265
AciI CCGC 9 cut(s) 402, 582, 585, 588, 591, 597, 611, 626, 971
AcoI YGGCCR 1 cut(s) 281
AcsI RAATTY 1 cut(s) 977
AcyI GRCGYC 4 cut(s) 411, 824, 890, 923
AfaI GTAC 2 cut(s) 767, 944
AfiI CCNNNNNNNGG 2 cut(s) 279, 779
AflIII ACRYGT 1 cut(s) 52
AgsI TTSAA 1 cut(s) 491
AhlI ACTAGT 1 cut(s) 356
AjiI CACGTC 2 cut(s) 190, 746
AleI CACNNNNGTG 1 cut(s) 563
AluBI AGCT 4 cut(s) 277, 509, 549, 650
AluI AGCT 4 cut(s) 277, 509, 549, 650
Alw21I GWGCWC 1 cut(s) 511
Alw26I GTCTC 3 cut(s) 43, 626, 878
AoxI GGCC 3 cut(s) 281, 770, 860
ApeKI GCWGC 1 cut(s) 706
ApoI RAATTY 1 cut(s) 977
AseI ATTAAT 1 cut(s) 867
AspLEI GCGC 1 cut(s) 826
AspS9I GGNCC 6 cut(s) 605, 623, 677, 770, 776, 860
AsuC2I CCSGG 2 cut(s) 774, 780
AsuHPI GGTGA 3 cut(s) 223, 419, 850
AsuII TTCGAA 1 cut(s) 60
AvaII GGWCC 4 cut(s) 605, 623, 677, 776
BalI TGGCCA 1 cut(s) 283
BanI GGYRCC 1 cut(s) 823
BanII GRGCYC 1 cut(s) 511
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 511
BbvI GCAGC 1 cut(s) 718
BccI CCATC 3 cut(s) 158, 497, 614
BceAI ACGGC 5 cut(s) 415, 811, 837, 903, 918
BciVI GTATCC 1 cut(s) 732
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 2 cut(s) 774, 780
BcoDI GTCTC 3 cut(s) 43, 626, 878
BcuI ACTAGT 1 cut(s) 356
BfaI CTAG 1 cut(s) 357
BfoI RGCGCY 1 cut(s) 827
BfuI GTATCC 1 cut(s) 732
BglII AGATCT 1 cut(s) 992
BisI GCNGC 4 cut(s) 583, 586, 589, 707
BlsI GCNGC 4 cut(s) 584, 587, 590, 708
Bme1390I CCNGG 2 cut(s) 774, 780
Bme18I GGWCC 4 cut(s) 605, 623, 677, 776
BmgBI CACGTC 2 cut(s) 190, 746
BmgT120I GGNCC 6 cut(s) 605, 623, 677, 770, 776, 860
BmiI GGNNCC 3 cut(s) 777, 825, 906
BmrFI CCNGG 2 cut(s) 774, 780
BmsI GCATC 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 375
Bpu10I CCTNAGC 1 cut(s) 550
Bpu14I TTCGAA 1 cut(s) 60
BpuMI CCSGG 2 cut(s) 774, 780
Bsa29I ATCGAT 1 cut(s) 162
BsaAI YACGTR 1 cut(s) 946
BsaBI GATNNNNATC 1 cut(s) 239
BsaHI GRCGYC 4 cut(s) 411, 824, 890, 923
BsaXI ACNNNNNCTCC 4 cut(s) 396, 426, 892, 922
Bsc4I CCNNNNNNNGG 2 cut(s) 279, 779
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 1 cut(s) 958
Bse8I GATNNNNATC 1 cut(s) 239
BseCI ATCGAT 1 cut(s) 162
BseGI GGATG 4 cut(s) 131, 715, 751, 916
BseJI GATNNNNATC 1 cut(s) 239
BseLI CCNNNNNNNGG 2 cut(s) 279, 779
BseMII CTCAG 2 cut(s) 660, 824
BseNI ACTGG 1 cut(s) 958
BseRI GAGGAG 4 cut(s) 450, 675, 780, 897
BseXI GCAGC 1 cut(s) 718
BshFI GGCC 3 cut(s) 283, 772, 862
BshNI GGYRCC 1 cut(s) 823
BshVI ATCGAT 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 511
BsiSI CCGG 4 cut(s) 230, 261, 773, 779
BslFI GGGAC 1 cut(s) 795
BslI CCNNNNNNNGG 2 cut(s) 279, 779
BsmAI GTCTC 3 cut(s) 43, 626, 878
BsmBI CGTCTC 1 cut(s) 878
BsmFI GGGAC 1 cut(s) 795
BsnI GGCC 3 cut(s) 283, 772, 862
Bsp119I TTCGAA 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 511
Bsp143I GATC 5 cut(s) 213, 249, 448, 658, 992
BspACI CCGC 9 cut(s) 402, 582, 585, 588, 591, 597, 611, 626, 971
BspANI GGCC 3 cut(s) 283, 772, 862
BspCNI CTCAG 2 cut(s) 659, 823
BspDI ATCGAT 1 cut(s) 162
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 3 cut(s) 777, 825, 906
BspT104I TTCGAA 1 cut(s) 60
BspT107I GGYRCC 1 cut(s) 823
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 1 cut(s) 958
BssAI RCCGGY 1 cut(s) 260
BssMI GATC 5 cut(s) 213, 249, 448, 658, 992
BssNI GRCGYC 4 cut(s) 411, 824, 890, 923
BstACI GRCGYC 4 cut(s) 411, 824, 890, 923
BstBAI YACGTR 1 cut(s) 946
BstBI TTCGAA 1 cut(s) 60
BstDEI CTNAG 5 cut(s) 80, 550, 646, 810, 1022
BstF5I GGATG 4 cut(s) 131, 715, 751, 916
BstH2I RGCGCY 1 cut(s) 827
BstHHI GCGC 1 cut(s) 826
BstKTI GATC 5 cut(s) 216, 252, 451, 661, 995
BstMAI GTCTC 3 cut(s) 43, 626, 878
BstMBI GATC 5 cut(s) 213, 249, 448, 658, 992
BstMWI GCNNNNNNNGC 2 cut(s) 588, 594
BstNSI RCATGY 1 cut(s) 56
BstSCI CCNGG 2 cut(s) 772, 778
BstV1I GCAGC 1 cut(s) 718
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 1 cut(s) 992
BstYI RGATCY 1 cut(s) 992
Bsu15I ATCGAT 1 cut(s) 162
BsuI GTATCC 1 cut(s) 732
BsuRI GGCC 3 cut(s) 283, 772, 862
BsuTUI ATCGAT 1 cut(s) 162
BtrI CACGTC 2 cut(s) 190, 746
BtsCI GGATG 4 cut(s) 131, 715, 751, 916
BtsIMutI CAGTG 2 cut(s) 642, 951
CciI TCATGA 1 cut(s) 252
CfoI GCGC 1 cut(s) 826
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 6 cut(s) 605, 623, 677, 770, 776, 860
ClaI ATCGAT 1 cut(s) 162
CseI GACGC 1 cut(s) 879
Csp6I GTAC 2 cut(s) 766, 943
CviAII CATG 4 cut(s) 53, 93, 253, 857
CviQI GTAC 2 cut(s) 766, 943
DdeI CTNAG 5 cut(s) 80, 550, 646, 810, 1022
DinI GGCGCC 1 cut(s) 825
DpnI GATC 5 cut(s) 215, 251, 450, 660, 994
DpnII GATC 5 cut(s) 213, 249, 448, 658, 992
EaeI YGGCCR 1 cut(s) 281
EciI GGCGGA 1 cut(s) 417
Ecl136II GAGCTC 1 cut(s) 509
Eco24I GRGCYC 1 cut(s) 511
Eco47I GGWCC 4 cut(s) 605, 623, 677, 776
Eco53kI GAGCTC 1 cut(s) 509
EcoICRI GAGCTC 1 cut(s) 509
EcoT38I GRGCYC 1 cut(s) 511
EgeI GGCGCC 1 cut(s) 825
EheI GGCGCC 1 cut(s) 825
Esp3I CGTCTC 1 cut(s) 878
FaeI CATG 4 cut(s) 56, 96, 256, 860
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FaqI GGGAC 1 cut(s) 795
FatI CATG 4 cut(s) 52, 92, 252, 856
FbaI TGATCA 1 cut(s) 448
FblI GTMKAC 1 cut(s) 265
Fnu4HI GCNGC 4 cut(s) 583, 586, 589, 707
FokI GGATG 4 cut(s) 138, 702, 738, 903
FriOI GRGCYC 1 cut(s) 511
Fsp4HI GCNGC 4 cut(s) 583, 586, 589, 707
FspBI CTAG 1 cut(s) 357
GlaI GCGC 1 cut(s) 825
GluI GCNGC 4 cut(s) 583, 586, 589, 707
HaeII RGCGCY 1 cut(s) 827
HaeIII GGCC 3 cut(s) 283, 772, 862
HapII CCGG 4 cut(s) 230, 261, 773, 779
HgaI GACGC 1 cut(s) 879
HhaI GCGC 1 cut(s) 826
Hin1I GRCGYC 4 cut(s) 411, 824, 890, 923
Hin1II CATG 4 cut(s) 56, 96, 256, 860
Hin6I GCGC 1 cut(s) 824
HinP1I GCGC 1 cut(s) 824
HincII GTYRAC 1 cut(s) 485
HindII GTYRAC 1 cut(s) 485
HindIII AAGCTT 1 cut(s) 547
HinfI GANTC 4 cut(s) 712, 962, 1007, 1016
HpaII CCGG 4 cut(s) 230, 261, 773, 779
HphI GGTGA 3 cut(s) 223, 419, 850
Hpy166II GTNNAC 5 cut(s) 266, 478, 485, 733, 949
Hpy188I TCNGA 6 cut(s) 28, 218, 327, 513, 921, 1006
Hpy188III TCNNGA 3 cut(s) 142, 244, 253
Hpy8I GTNNAC 5 cut(s) 266, 478, 485, 733, 949
Hpy99I CGWCG 8 cut(s) 191, 377, 380, 416, 565, 895, 925, 1006
HpyAV CCTTC 5 cut(s) 116, 569, 804, 921, 1022
HpyCH4IV ACGT 8 cut(s) 189, 222, 287, 411, 470, 745, 923, 945
HpyCH4V TGCA 2 cut(s) 129, 856
HpyF10VI GCNNNNNNNGC 2 cut(s) 588, 594
HpyF3I CTNAG 5 cut(s) 80, 550, 646, 810, 1022
HpySE526I ACGT 8 cut(s) 189, 222, 287, 411, 470, 745, 923, 945
Hsp92I GRCGYC 4 cut(s) 411, 824, 890, 923
Hsp92II CATG 4 cut(s) 56, 96, 256, 860
HspAI GCGC 1 cut(s) 824
KasI GGCGCC 1 cut(s) 823
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 5 cut(s) 213, 249, 448, 658, 992
LmnI GCTCC 4 cut(s) 506, 514, 655, 910
LpnPI CCDG 8 cut(s) 84, 243, 274, 437, 642, 786, 792, 939
Lsp1109I GCAGC 1 cut(s) 718
LweI GCATC 1 cut(s) 116
MaeI CTAG 1 cut(s) 357
MaeII ACGT 8 cut(s) 189, 222, 287, 411, 470, 745, 923, 945
MaeIII GTNAC 6 cut(s) 205, 317, 407, 466, 842, 1017
MalI GATC 5 cut(s) 215, 251, 450, 660, 994
MboI GATC 5 cut(s) 213, 249, 448, 658, 992
MflI RGATCY 1 cut(s) 992
MhlI GDGCHC 1 cut(s) 511
MlsI TGGCCA 1 cut(s) 283
MluCI AATT 2 cut(s) 864, 977
MluNI TGGCCA 1 cut(s) 283
Mly113I GGCGCC 1 cut(s) 824
MlyI GAGTC 1 cut(s) 1025
MmeI TCCRAC 3 cut(s) 749, 944, 984
Mox20I TGGCCA 1 cut(s) 283
MscI TGGCCA 1 cut(s) 283
MseI TTAA 4 cut(s) 239, 306, 456, 867
MslI CAYNNNNRTG 1 cut(s) 563
Msp20I TGGCCA 1 cut(s) 283
MspI CCGG 4 cut(s) 230, 261, 773, 779
MspR9I CCNGG 2 cut(s) 774, 780
MwoI GCNNNNNNNGC 2 cut(s) 588, 594
NarI GGCGCC 1 cut(s) 824
NciI CCSGG 2 cut(s) 774, 780
NdeII GATC 5 cut(s) 213, 249, 448, 658, 992
NlaIII CATG 4 cut(s) 56, 96, 256, 860
NlaIV GGNNCC 3 cut(s) 777, 825, 906
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 2 cut(s) 407, 1017
NspI RCATGY 1 cut(s) 56
NspV TTCGAA 1 cut(s) 60
OliI CACNNNNGTG 1 cut(s) 563
PagI TCATGA 1 cut(s) 252
PciI ACATGT 1 cut(s) 52
PfeI GAWTC 3 cut(s) 712, 962, 1007
PflFI GACNNNGTC 1 cut(s) 889
PfoI TCCNGGA 1 cut(s) 778
PkrI GCNGC 4 cut(s) 584, 587, 590, 708
PleI GAGTC 1 cut(s) 1024
PluTI GGCGCC 1 cut(s) 827
PpsI GAGTC 1 cut(s) 1024
Ppu21I YACGTR 1 cut(s) 946
PscI ACATGT 1 cut(s) 52
PshBI ATTAAT 1 cut(s) 867
Psp124BI GAGCTC 1 cut(s) 511
PspN4I GGNNCC 3 cut(s) 777, 825, 906
PspPI GGNCC 6 cut(s) 605, 623, 677, 770, 776, 860
PsuI RGATCY 1 cut(s) 992
PsyI GACNNNGTC 1 cut(s) 889
RsaI GTAC 2 cut(s) 767, 944
RsaNI GTAC 2 cut(s) 766, 943
RseI CAYNNNNRTG 1 cut(s) 563
SacI GAGCTC 1 cut(s) 511
SaqAI TTAA 4 cut(s) 239, 306, 456, 867
SatI GCNGC 4 cut(s) 583, 586, 589, 707
Sau3AI GATC 5 cut(s) 213, 249, 448, 658, 992
Sau96I GGNCC 6 cut(s) 605, 623, 677, 770, 776, 860
SchI GAGTC 1 cut(s) 1025
ScrFI CCNGG 2 cut(s) 774, 780
SduI GDGCHC 1 cut(s) 511
SfaNI GCATC 1 cut(s) 116
SfoI GGCGCC 1 cut(s) 825
SfuI TTCGAA 1 cut(s) 60
SinI GGWCC 4 cut(s) 605, 623, 677, 776
SmiMI CAYNNNNRTG 1 cut(s) 563
SpeI ACTAGT 1 cut(s) 356
Sse9I AATT 2 cut(s) 864, 977
SsiI CCGC 9 cut(s) 402, 582, 585, 588, 591, 597, 611, 626, 971
SspDI GGCGCC 1 cut(s) 823
SspI AATATT 1 cut(s) 150
SspMI CTAG 1 cut(s) 357
SstI GAGCTC 1 cut(s) 511
StyD4I CCNGG 2 cut(s) 772, 778
TaiI ACGT 8 cut(s) 192, 225, 290, 414, 473, 748, 926, 948
TaqI TCGA 3 cut(s) 60, 162, 186
TasI AATT 2 cut(s) 864, 977
TauI GCSGC 3 cut(s) 585, 588, 591
TfiI GAWTC 3 cut(s) 712, 962, 1007
Tru1I TTAA 4 cut(s) 239, 306, 456, 867
Tru9I TTAA 4 cut(s) 239, 306, 456, 867
TscAI CASTG 2 cut(s) 642, 958
TseFI GTSAC 2 cut(s) 407, 1017
TseI GCWGC 1 cut(s) 706
Tsp45I GTSAC 2 cut(s) 407, 1017
TspDTI ATGAA 2 cut(s) 269, 381
TspGWI ACGGA 2 cut(s) 690, 708
TspRI CASTG 2 cut(s) 642, 958
Tth111I GACNNNGTC 1 cut(s) 889
VpaK11BI GGWCC 4 cut(s) 605, 623, 677, 776
VspI ATTAAT 1 cut(s) 867
XapI RAATTY 1 cut(s) 977
XceI RCATGY 1 cut(s) 56
XmiI GTMKAC 1 cut(s) 265
XspI CTAG 1 cut(s) 357
ZraI GACGTC 2 cut(s) 412, 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.