Rorug06G0235000

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011694
Physical Location & Seq
Reverse (-)
37767505 .. 37768240
736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0235000.1

Sequence Viewer

Length: 540 bp
ATGTTCATCTGTGCTGACTTTGGTGGTGTGTTGGTTCTGTTTTTCAGAGCTGAGAACTCTGACACACATAAGCATCTATGTGAGTTCACTGGTCTCGATATTGAGATGGAGATTGAGTGGAGCTATTTTGAGATTATGGATATTGTTAACAGAGAGGAAGTTGGGCCAAATAGTTTTGGAGAATATGGCACTGAGTTCTACATACTTCACCGCTATCCGTTGGCTGTGAGGCCTTTCTATACCATGCCTTGCGACGATAATCCTGAATACAGTAATTCGTTTAACGTCTTCATTCGAGGTGAGGAGATTATATCAGGGGCACAACGTATCCATGTACCAGAATTATTGGTCGAACGTGCTGAAGCACGTGGAATTGAAGTTGAGACAGTATCAACATATATTGATTCCTTCAGATATGGTGCACCTCTCCATGGTGGATTTGGAGTAGGGCTGGAGCATGTTGTGATGCTCTTCTGTGGTTTGAACAACATCCGCAAAACATCTTTATTCCCGCGTGATCCAGACCGGCTAGCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

179

Amino Acids

20.5

Weight (kDa)

4.72

Isoelectric Point (pI)

36.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_2 PF00152 62 - 173 3.2e-27 tRNA synthetases class II (D, K and N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011694)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27690
fragaria_vesca FvH4_2g25240 FvH4_3g05420
malus_domestica MD03G1282300.v1.1 MD11G1304000.v1.1
prunus_persica Prupe.8G257200_v2.0.a1
pyrus_communis pycom03g22310 pycom11g26560
rosa_chinensis RchiOBHm_Chr6g0293381
rosa_laevigata RLG00000011951
rosa_multiflora Rmu_sc0006269.1_g000028
rosa_roxburghii Rroxscaffold_7G00174210
rosa_rugosa Rorug06G0235000 Rorug06G0235100
rosa_samantha Rh6AG347900 Rh6BG355500 Rh6CG361600 Rh6DG348700
rosa_wichuraiana Rw6G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 514
AciI CCGC 3 cut(s) 211, 493, 512
AclWI GGATC 1 cut(s) 512
AcuI CTGAAG 2 cut(s) 381, 394
AcvI CACGTG 1 cut(s) 368
AfaI GTAC 1 cut(s) 336
AfiI CCNNNNNNNGG 1 cut(s) 431
AgsI TTSAA 2 cut(s) 377, 484
AluBI AGCT 3 cut(s) 50, 123, 533
AluI AGCT 3 cut(s) 50, 123, 533
Alw21I GWGCWC 1 cut(s) 424
Alw26I GTCTC 2 cut(s) 98, 377
Alw44I GTGCAC 1 cut(s) 420
AlwI GGATC 1 cut(s) 512
AoxI GGCC 2 cut(s) 164, 230
ApaLI GTGCAC 1 cut(s) 420
AspS9I GGNCC 1 cut(s) 164
AsuHPI GGTGA 2 cut(s) 200, 311
AsuNHI GCTAGC 1 cut(s) 529
BaeGI GKGCMC 2 cut(s) 322, 424
BbrPI CACGTG 1 cut(s) 368
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 424
BccI CCATC 1 cut(s) 100
BciVI GTATCC 1 cut(s) 338
BcoDI GTCTC 2 cut(s) 98, 377
BfaI CTAG 1 cut(s) 530
BfuI GTATCC 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 164
BmsI GCATC 2 cut(s) 82, 456
BmtI GCTAGC 1 cut(s) 533
BpiI GAAGAC 1 cut(s) 280
BpmI CTGGAG 1 cut(s) 473
BsaAI YACGTR 1 cut(s) 368
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 1 cut(s) 430
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 431
Bse118I RCCGGY 1 cut(s) 525
Bse1I ACTGG 1 cut(s) 94
BseDI CCNNGG 1 cut(s) 430
BseGI GGATG 1 cut(s) 489
BseLI CCNNNNNNNGG 1 cut(s) 431
BseMII CTCAG 2 cut(s) 42, 183
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 1 cut(s) 317
BseSI GKGCMC 2 cut(s) 322, 424
Bsh1236I CGCG 1 cut(s) 514
BshFI GGCC 2 cut(s) 166, 232
BsiHKAI GWGCWC 1 cut(s) 424
BsiSI CCGG 1 cut(s) 526
BslI CCNNNNNNNGG 1 cut(s) 431
BsmAI GTCTC 2 cut(s) 98, 377
BsnI GGCC 2 cut(s) 166, 232
Bso31I GGTCTC 1 cut(s) 98
Bsp1286I GDGCHC 2 cut(s) 322, 424
Bsp143I GATC 1 cut(s) 517
Bsp19I CCATGG 1 cut(s) 430
BspACI CCGC 3 cut(s) 211, 493, 512
BspANI GGCC 2 cut(s) 166, 232
BspCNI CTCAG 2 cut(s) 43, 184
BspFNI CGCG 1 cut(s) 514
BspOI GCTAGC 1 cut(s) 533
BspPI GGATC 1 cut(s) 512
BspQI GCTCTTC 1 cut(s) 476
BspTNI GGTCTC 1 cut(s) 98
BsrFI RCCGGY 1 cut(s) 525
BsrI ACTGG 1 cut(s) 94
BssAI RCCGGY 1 cut(s) 525
BssECI CCNNGG 1 cut(s) 430
BssMI GATC 1 cut(s) 517
BssT1I CCWWGG 1 cut(s) 430
Bst4CI ACNGT 2 cut(s) 272, 388
Bst6I CTCTTC 1 cut(s) 476
BstBAI YACGTR 1 cut(s) 368
BstC8I GCNNGC 1 cut(s) 531
BstDEI CTNAG 2 cut(s) 51, 192
BstDSI CCRYGG 1 cut(s) 430
BstF5I GGATG 1 cut(s) 489
BstFNI CGCG 1 cut(s) 514
BstKTI GATC 1 cut(s) 520
BstMAI GTCTC 2 cut(s) 98, 377
BstMBI GATC 1 cut(s) 517
BstNSI RCATGY 1 cut(s) 461
BstSLI GKGCMC 2 cut(s) 322, 424
BstUI CGCG 1 cut(s) 514
BstV2I GAAGAC 1 cut(s) 280
BsuI GTATCC 1 cut(s) 338
BsuRI GGCC 2 cut(s) 166, 232
BtgI CCRYGG 1 cut(s) 430
BtsCI GGATG 1 cut(s) 489
BtsIMutI CAGTG 2 cut(s) 87, 189
Cac8I GCNNGC 1 cut(s) 531
Cfr10I RCCGGY 1 cut(s) 525
Cfr13I GGNCC 1 cut(s) 164
Csp6I GTAC 1 cut(s) 335
CviAII CATG 4 cut(s) 244, 332, 431, 458
CviJI RGCY 8 cut(s) 50, 123, 166, 224, 232, 451, 529, 533
CviKI_1 RGCY 8 cut(s) 50, 123, 166, 224, 232, 451, 529, 533
CviQI GTAC 1 cut(s) 335
DdeI CTNAG 2 cut(s) 51, 192
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
Eam1104I CTCTTC 1 cut(s) 476
EarI CTCTTC 1 cut(s) 476
Eco130I CCWWGG 1 cut(s) 430
Eco147I AGGCCT 1 cut(s) 232
Eco31I GGTCTC 1 cut(s) 98
Eco57I CTGAAG 2 cut(s) 381, 394
Eco72I CACGTG 1 cut(s) 368
EcoT14I CCWWGG 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 430
FaeI CATG 4 cut(s) 247, 335, 434, 461
FatI CATG 4 cut(s) 243, 331, 430, 457
FauI CCCGC 1 cut(s) 519
FokI GGATG 1 cut(s) 476
FspBI CTAG 1 cut(s) 530
GsuI CTGGAG 1 cut(s) 473
HaeIII GGCC 2 cut(s) 166, 232
HapII CCGG 1 cut(s) 526
Hin1II CATG 4 cut(s) 247, 335, 434, 461
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 1 cut(s) 404
HpaI GTTAAC 1 cut(s) 148
HpaII CCGG 1 cut(s) 526
HphI GGTGA 2 cut(s) 200, 311
Hpy166II GTNNAC 3 cut(s) 87, 148, 422
Hpy188I TCNGA 3 cut(s) 47, 61, 413
Hpy188III TCNNGA 3 cut(s) 95, 263, 521
Hpy8I GTNNAC 3 cut(s) 87, 148, 422
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 2 cut(s) 272, 388
HpyCH4IV ACGT 4 cut(s) 285, 325, 355, 367
HpyCH4V TGCA 1 cut(s) 422
HpyF3I CTNAG 2 cut(s) 51, 192
HpySE526I ACGT 4 cut(s) 285, 325, 355, 367
Hsp92II CATG 4 cut(s) 247, 335, 434, 461
KspAI GTTAAC 1 cut(s) 148
Kzo9I GATC 1 cut(s) 517
LguI GCTCTTC 1 cut(s) 476
LmnI GCTCC 3 cut(s) 120, 454, 538
LpnPI CCDG 6 cut(s) 75, 276, 300, 351, 437, 534
LweI GCATC 2 cut(s) 82, 456
MaeI CTAG 1 cut(s) 530
MaeII ACGT 4 cut(s) 285, 325, 355, 367
MalI GATC 1 cut(s) 519
MboI GATC 1 cut(s) 517
MboII GAAGA 2 cut(s) 280, 463
MhlI GDGCHC 2 cut(s) 322, 424
MluCI AATT 3 cut(s) 274, 341, 372
MnlI CCTC 5 cut(s) 148, 222, 290, 295, 435
MseI TTAA 2 cut(s) 147, 282
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 1 cut(s) 526
MvnI CGCG 1 cut(s) 514
NcoI CCATGG 1 cut(s) 430
NdeII GATC 1 cut(s) 517
NheI GCTAGC 1 cut(s) 529
NlaIII CATG 4 cut(s) 247, 335, 434, 461
NspI RCATGY 1 cut(s) 461
PceI AGGCCT 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 476
PfeI GAWTC 1 cut(s) 404
PmaCI CACGTG 1 cut(s) 368
PmlI CACGTG 1 cut(s) 368
Ppu21I YACGTR 1 cut(s) 368
PspCI CACGTG 1 cut(s) 368
PspPI GGNCC 1 cut(s) 164
RsaI GTAC 1 cut(s) 336
RsaNI GTAC 1 cut(s) 335
RseI CAYNNNNRTG 1 cut(s) 78
SapI GCTCTTC 1 cut(s) 476
SaqAI TTAA 2 cut(s) 147, 282
Sau3AI GATC 1 cut(s) 517
Sau96I GGNCC 1 cut(s) 164
SduI GDGCHC 2 cut(s) 322, 424
SetI ASST 9 cut(s) 52, 125, 288, 301, 328, 358, 370, 427, 535
SfaNI GCATC 2 cut(s) 82, 456
SmiMI CAYNNNNRTG 1 cut(s) 78
Sse9I AATT 3 cut(s) 274, 341, 372
SseBI AGGCCT 1 cut(s) 232
SsiI CCGC 3 cut(s) 211, 493, 512
SspMI CTAG 1 cut(s) 530
StuI AGGCCT 1 cut(s) 232
StyI CCWWGG 1 cut(s) 430
TaaI ACNGT 2 cut(s) 272, 388
TaiI ACGT 4 cut(s) 288, 328, 358, 370
TaqI TCGA 3 cut(s) 96, 295, 351
TasI AATT 3 cut(s) 274, 341, 372
TfiI GAWTC 1 cut(s) 404
Tru1I TTAA 2 cut(s) 147, 282
Tru9I TTAA 2 cut(s) 147, 282
TscAI CASTG 2 cut(s) 94, 196
TspDTI ATGAA 1 cut(s) 280
TspGWI ACGGA 1 cut(s) 207
TspRI CASTG 2 cut(s) 94, 196
VneI GTGCAC 1 cut(s) 420
XceI RCATGY 1 cut(s) 461
XcmI CCANNNNNNNNNTGG 1 cut(s) 437
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.