Rmu_sc0006269.1_g000028

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006269.1
OG0011694
Physical Location & Seq
Reverse (-)
119354 .. 120477
1124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006269.1_g000028.1.cds

Sequence Viewer

Length: 864 bp
atggcaacaaaaccagctgaagaacctcctggatccttgaaataccagacatgggtgttgaaagtctcgattcattgtgaaggctgcaaaaggaaagtcaagaaagttcttcaaagcattgaaggggtttacactattgcgattgattctcagcagcacaaggtcacagtgactggcaacgtgggggcagagaccttactcaagaagctgttgaagtcgggcaagcacgccgagctctggccagaacctaagaaggggaagaagtccaagaagaagaaagataatggggaagcagatggtgagaatggtgatgaagcgaaaaattctgctgagaaagaccaacaagctgatgaaaatggtggtgatgatgctgatgatgatgacggtgagtcagacaaagagggtgggaatcaaaacaatgaagctggtggagaacaaagtgctggtgctggtgcttcaactggagccaaaaagaagaaaaagaaaaagaagaagaaagcgcaaaacgggaatcctcctaatggtggcactggtatccccagtgagcatttaggtgatgctcatccggcgggtgctaatgggccgcctatggttggtccagacatttctcacccaatggctgccatgaatctcggcccaccgatccaacatgtatacccgggtccatacccgcgaccgatgtattatcagcctccggccgcatatggtctaagttacaatacgacgtatccgagctcaagtgcttcatattttacccctgctatgcatgaaaacacgtactatcacccggacatctactcgccttctgatcctgttgattcgtttacggacgatgaagaagaaagtggctgctacattatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

287

Amino Acids

30.73

Weight (kDa)

5.88

Isoelectric Point (pI)

30.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011694)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27690
fragaria_vesca FvH4_2g25240 FvH4_3g05420
malus_domestica MD03G1282300.v1.1 MD11G1304000.v1.1
prunus_persica Prupe.8G257200_v2.0.a1
pyrus_communis pycom03g22310 pycom11g26560
rosa_chinensis RchiOBHm_Chr6g0293381
rosa_laevigata RLG00000011951
rosa_multiflora Rmu_sc0006269.1_g000028
rosa_roxburghii Rroxscaffold_7G00174210
rosa_rugosa Rorug06G0235000 Rorug06G0235100
rosa_samantha Rh6AG347900 Rh6BG355500 Rh6CG361600 Rh6DG348700
rosa_wichuraiana Rw6G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 654
AccII CGCG 1 cut(s) 673
AciI CCGC 4 cut(s) 569, 584, 671, 699
AclWI GGATC 4 cut(s) 27, 40, 637, 803
AcoI YGGCCR 2 cut(s) 239, 696
AcsI RAATTY 1 cut(s) 322
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 1 cut(s) 779
AfiI CCNNNNNNNGG 6 cut(s) 52, 237, 254, 521, 524, 593
AflIII ACRYGT 2 cut(s) 649, 774
AgsI TTSAA 6 cut(s) 40, 61, 113, 122, 214, 459
AhdI GACNNNNNGTC 1 cut(s) 388
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 6 cut(s) 17, 208, 235, 347, 425, 735
AluI AGCT 6 cut(s) 17, 208, 235, 347, 425, 735
Alw21I GWGCWC 2 cut(s) 237, 737
Alw26I GTCTC 2 cut(s) 70, 185
AlwI GGATC 4 cut(s) 27, 40, 637, 803
AlwNI CAGNNNCTG 1 cut(s) 173
Ama87I CYCGRG 1 cut(s) 658
AoxI GGCC 4 cut(s) 239, 581, 634, 696
ApeKI GCWGC 4 cut(s) 84, 154, 620, 849
ApoI RAATTY 1 cut(s) 322
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 4 cut(s) 581, 596, 635, 662
AsuC2I CCSGG 3 cut(s) 659, 660, 788
AsuHPI GGTGA 7 cut(s) 311, 320, 374, 398, 566, 602, 776
AvaI CYCGRG 1 cut(s) 658
AvaII GGWCC 2 cut(s) 596, 662
BalI TGGCCA 1 cut(s) 241
BamHI GGATCC 1 cut(s) 32
BanII GRGCYC 2 cut(s) 237, 737
Bbv12I GWGCWC 2 cut(s) 237, 737
BbvI GCAGC 4 cut(s) 71, 166, 607, 836
BccI CCATC 1 cut(s) 290
BciT130I CCWGG 1 cut(s) 30
BciVI GTATCC 2 cut(s) 545, 738
BcnI CCSGG 3 cut(s) 659, 660, 788
BcoDI GTCTC 2 cut(s) 70, 185
BfuI GTATCC 2 cut(s) 545, 738
BisI GCNGC 6 cut(s) 85, 155, 584, 621, 699, 850
BlsI GCNGC 6 cut(s) 86, 156, 585, 622, 700, 851
Bme1390I CCNGG 4 cut(s) 30, 659, 660, 788
Bme18I GGWCC 2 cut(s) 596, 662
BmeRI GACNNNNNGTC 1 cut(s) 388
BmeT110I CYCGRG 1 cut(s) 658
BmgT120I GGNCC 4 cut(s) 581, 596, 635, 662
BmiI GGNNCC 3 cut(s) 34, 466, 663
BmrFI CCNGG 4 cut(s) 30, 659, 660, 788
BmrI ACTGGG 1 cut(s) 534
BmsI GCATC 2 cut(s) 358, 547
BmuI ACTGGG 1 cut(s) 534
BpmI CTGGAG 1 cut(s) 483
BpuEI CTTGAG 2 cut(s) 185, 721
BpuMI CCSGG 3 cut(s) 659, 660, 788
BsaAI YACGTR 1 cut(s) 777
BsaBI GATNNNNATC 1 cut(s) 561
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 658
Bsc4I CCNNNNNNNGG 6 cut(s) 52, 237, 254, 521, 524, 593
Bse1I ACTGG 4 cut(s) 178, 466, 535, 540
Bse8I GATNNNNATC 1 cut(s) 561
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 658
BseGI GGATG 1 cut(s) 562
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 6 cut(s) 52, 237, 254, 521, 524, 593
BseMII CTCAG 2 cut(s) 164, 321
BseNI ACTGG 4 cut(s) 178, 466, 535, 540
BseX3I CGGCCG 1 cut(s) 696
BseXI GCAGC 4 cut(s) 71, 166, 607, 836
Bsh1236I CGCG 1 cut(s) 673
Bsh1285I CGRYCG 2 cut(s) 677, 699
BshFI GGCC 4 cut(s) 241, 583, 636, 698
BsiEI CGRYCG 2 cut(s) 677, 699
BsiHKAI GWGCWC 2 cut(s) 237, 737
BsiHKCI CYCGRG 1 cut(s) 658
BsiSI CCGG 4 cut(s) 566, 659, 695, 788
BslI CCNNNNNNNGG 6 cut(s) 52, 237, 254, 521, 524, 593
BsmAI GTCTC 2 cut(s) 70, 185
BsnI GGCC 4 cut(s) 241, 583, 636, 698
Bso31I GGTCTC 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 658
Bsp1286I GDGCHC 2 cut(s) 237, 737
Bsp143I GATC 3 cut(s) 32, 642, 808
BspACI CCGC 4 cut(s) 569, 584, 671, 699
BspANI GGCC 4 cut(s) 241, 583, 636, 698
BspCNI CTCAG 2 cut(s) 163, 322
BspFNI CGCG 1 cut(s) 673
BspLI GGNNCC 3 cut(s) 34, 466, 663
BspPI GGATC 4 cut(s) 27, 40, 637, 803
BspTNI GGTCTC 1 cut(s) 185
BsrI ACTGG 4 cut(s) 178, 466, 535, 540
BssECI CCNNGG 1 cut(s) 658
BssMI GATC 3 cut(s) 32, 642, 808
BssNAI GTATAC 1 cut(s) 655
Bst1107I GTATAC 1 cut(s) 655
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 169, 386
BstBAI YACGTR 1 cut(s) 777
BstC8I GCNNGC 2 cut(s) 224, 228
BstDEI CTNAG 4 cut(s) 150, 249, 330, 710
BstF5I GGATG 1 cut(s) 562
BstFNI CGCG 1 cut(s) 673
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 3 cut(s) 35, 645, 811
BstMAI GTCTC 2 cut(s) 70, 185
BstMBI GATC 3 cut(s) 32, 642, 808
BstMCI CGRYCG 2 cut(s) 677, 699
BstMWI GCNNNNNNNGC 2 cut(s) 232, 566
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 653
BstSCI CCNGG 4 cut(s) 28, 657, 658, 786
BstUI CGCG 1 cut(s) 673
BstV1I GCAGC 4 cut(s) 71, 166, 607, 836
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
BstZ17I GTATAC 1 cut(s) 655
BstZI CGGCCG 1 cut(s) 696
BsuI GTATCC 2 cut(s) 545, 738
BsuRI GGCC 4 cut(s) 241, 583, 636, 698
BtsCI GGATG 1 cut(s) 562
BtsIMutI CAGTG 3 cut(s) 174, 528, 547
Cac8I GCNNGC 2 cut(s) 224, 228
CaiI CAGNNNCTG 1 cut(s) 173
CfoI GCGC 1 cut(s) 502
Cfr13I GGNCC 4 cut(s) 581, 596, 635, 662
Cfr9I CCCGGG 1 cut(s) 658
Csp6I GTAC 1 cut(s) 778
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviAII CATG 4 cut(s) 51, 625, 650, 767
CviQI GTAC 1 cut(s) 778
DdeI CTNAG 4 cut(s) 150, 249, 330, 710
DpnI GATC 3 cut(s) 34, 644, 810
DpnII GATC 3 cut(s) 32, 642, 808
DriI GACNNNNNGTC 1 cut(s) 388
EaeI YGGCCR 2 cut(s) 239, 696
EagI CGGCCG 1 cut(s) 696
Eam1105I GACNNNNNGTC 1 cut(s) 388
Ecl136II GAGCTC 2 cut(s) 235, 735
EclXI CGGCCG 1 cut(s) 696
Eco24I GRGCYC 2 cut(s) 237, 737
Eco31I GGTCTC 1 cut(s) 185
Eco47I GGWCC 2 cut(s) 596, 662
Eco52I CGGCCG 1 cut(s) 696
Eco53kI GAGCTC 2 cut(s) 235, 735
Eco57I CTGAAG 1 cut(s) 39
Eco88I CYCGRG 1 cut(s) 658
EcoICRI GAGCTC 2 cut(s) 235, 735
EcoRII CCWGG 1 cut(s) 28
EcoT22I ATGCAT 1 cut(s) 768
EcoT38I GRGCYC 2 cut(s) 237, 737
FaeI CATG 4 cut(s) 54, 628, 653, 770
FatI CATG 4 cut(s) 50, 624, 649, 766
FauI CCCGC 2 cut(s) 562, 678
FauNDI CATATG 1 cut(s) 703
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 6 cut(s) 85, 155, 584, 621, 699, 850
FokI GGATG 1 cut(s) 549
FriOI GRGCYC 2 cut(s) 237, 737
Fsp4HI GCNGC 6 cut(s) 85, 155, 584, 621, 699, 850
GlaI GCGC 1 cut(s) 501
GluI GCNGC 6 cut(s) 85, 155, 584, 621, 699, 850
GsuI CTGGAG 1 cut(s) 483
HaeIII GGCC 4 cut(s) 241, 583, 636, 698
HapII CCGG 4 cut(s) 566, 659, 695, 788
HhaI GCGC 1 cut(s) 502
Hin1II CATG 4 cut(s) 54, 628, 653, 770
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HinfI GANTC 7 cut(s) 70, 146, 389, 409, 511, 628, 818
HpaII CCGG 4 cut(s) 566, 659, 695, 788
HphI GGTGA 7 cut(s) 311, 320, 374, 398, 566, 602, 776
Hpy166II GTNNAC 3 cut(s) 130, 655, 825
Hpy188I TCNGA 3 cut(s) 394, 732, 808
Hpy188III TCNNGA 4 cut(s) 67, 100, 202, 599
Hpy8I GTNNAC 3 cut(s) 130, 655, 825
Hpy99I CGWCG 1 cut(s) 727
HpyAV CCTTC 4 cut(s) 74, 116, 247, 813
HpyCH4III ACNGT 2 cut(s) 169, 386
HpyCH4IV ACGT 3 cut(s) 180, 725, 776
HpyCH4V TGCA 2 cut(s) 87, 766
HpyF10VI GCNNNNNNNGC 2 cut(s) 232, 566
HpyF3I CTNAG 4 cut(s) 150, 249, 330, 710
HpySE526I ACGT 3 cut(s) 180, 725, 776
Hsp92II CATG 4 cut(s) 54, 628, 653, 770
HspAI GCGC 1 cut(s) 500
Kzo9I GATC 3 cut(s) 32, 642, 808
LmnI GCTCC 1 cut(s) 464
Lsp1109I GCAGC 4 cut(s) 71, 166, 607, 836
LweI GCATC 2 cut(s) 358, 547
MaeII ACGT 3 cut(s) 180, 725, 776
MaeIII GTNAC 3 cut(s) 163, 169, 713
MalI GATC 3 cut(s) 34, 644, 810
MboI GATC 3 cut(s) 32, 642, 808
MflI RGATCY 1 cut(s) 32
MhlI GDGCHC 2 cut(s) 237, 737
MlsI TGGCCA 1 cut(s) 241
MluCI AATT 1 cut(s) 322
MluNI TGGCCA 1 cut(s) 241
MlyI GAGTC 1 cut(s) 398
MmeI TCCRAC 1 cut(s) 670
MnlI CCTC 4 cut(s) 36, 394, 525, 702
Mox20I TGGCCA 1 cut(s) 241
Mph1103I ATGCAT 1 cut(s) 768
MscI TGGCCA 1 cut(s) 241
MslI CAYNNNNRTG 1 cut(s) 552
Msp20I TGGCCA 1 cut(s) 241
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 4 cut(s) 566, 659, 695, 788
MspR9I CCNGG 4 cut(s) 30, 659, 660, 788
MvaI CCWGG 1 cut(s) 30
MvnI CGCG 1 cut(s) 673
MwoI GCNNNNNNNGC 2 cut(s) 232, 566
NciI CCSGG 3 cut(s) 659, 660, 788
NdeI CATATG 1 cut(s) 703
NdeII GATC 3 cut(s) 32, 642, 808
NlaIII CATG 4 cut(s) 54, 628, 653, 770
NlaIV GGNNCC 3 cut(s) 34, 466, 663
NmeAIII GCCGAG 2 cut(s) 256, 612
NmuCI GTSAC 2 cut(s) 163, 169
NsiI ATGCAT 1 cut(s) 768
NspI RCATGY 1 cut(s) 653
PciI ACATGT 1 cut(s) 649
PcsI WCGNNNNNNNCGW 1 cut(s) 728
PfeI GAWTC 6 cut(s) 70, 146, 409, 511, 628, 818
PfoI TCCNGGA 1 cut(s) 28
PkrI GCNGC 6 cut(s) 86, 156, 585, 622, 700, 851
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
Ppu21I YACGTR 1 cut(s) 777
PscI ACATGT 1 cut(s) 649
Psp124BI GAGCTC 2 cut(s) 237, 737
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 3 cut(s) 34, 466, 663
PspPI GGNCC 4 cut(s) 581, 596, 635, 662
PstNI CAGNNNCTG 1 cut(s) 173
PsuI RGATCY 1 cut(s) 32
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 1 cut(s) 779
RsaNI GTAC 1 cut(s) 778
RseI CAYNNNNRTG 1 cut(s) 552
SacI GAGCTC 2 cut(s) 237, 737
SatI GCNGC 6 cut(s) 85, 155, 584, 621, 699, 850
Sau3AI GATC 3 cut(s) 32, 642, 808
Sau96I GGNCC 4 cut(s) 581, 596, 635, 662
SchI GAGTC 1 cut(s) 398
ScrFI CCNGG 4 cut(s) 30, 659, 660, 788
SduI GDGCHC 2 cut(s) 237, 737
SfaNI GCATC 2 cut(s) 358, 547
SinI GGWCC 2 cut(s) 596, 662
SmaI CCCGGG 1 cut(s) 660
SmiMI CAYNNNNRTG 1 cut(s) 552
SmlI CTYRAG 2 cut(s) 200, 736
SmoI CTYRAG 2 cut(s) 200, 736
Sse9I AATT 1 cut(s) 322
SsiI CCGC 4 cut(s) 569, 584, 671, 699
SstI GAGCTC 2 cut(s) 237, 737
StyD4I CCNGG 4 cut(s) 28, 657, 658, 786
TaaI ACNGT 2 cut(s) 169, 386
TaiI ACGT 3 cut(s) 183, 728, 779
TaqI TCGA 1 cut(s) 68
TaqII GACCGA 1 cut(s) 691
TasI AATT 1 cut(s) 322
TauI GCSGC 2 cut(s) 586, 701
TfiI GAWTC 6 cut(s) 70, 146, 409, 511, 628, 818
TscAI CASTG 3 cut(s) 174, 535, 547
TseFI GTSAC 2 cut(s) 163, 169
TseI GCWGC 4 cut(s) 84, 154, 620, 849
Tsp45I GTSAC 2 cut(s) 163, 169
TspDTI ATGAA 8 cut(s) 62, 327, 366, 435, 641, 735, 783, 849
TspGWI ACGGA 1 cut(s) 842
TspMI CCCGGG 1 cut(s) 658
TspRI CASTG 3 cut(s) 174, 535, 547
VpaK11BI GGWCC 2 cut(s) 596, 662
XapI RAATTY 1 cut(s) 322
XceI RCATGY 1 cut(s) 653
XmaI CCCGGG 1 cut(s) 658
XmiI GTMKAC 1 cut(s) 654
Zsp2I ATGCAT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.