RLG00000011951

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011694
Physical Location & Seq
Reverse (-)
14012069 .. 14013201
1133 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011951

Sequence Viewer

Length: 870 bp
ATGGCAACAAAACCAGCTGAAGAACCTCCTGGATCCTTGAAATACCAGACATGGGTATTGAAAGTCTCGATTCATTGTGAAGGCTGCAAAAGGAAAGTCAAGAAAGTTCTTCAAAGCATTGAAGGGGTTTACACTATTGCGATTGATTCTCAGCAGCACAAGGTCACAGTGACTGGCAACGTGGGGGCAGAGACCTTACTCAAGAAGCTGTTGAAGTCGGGCAAGCACGCCGAGCTCTGGCCAGAACCTAAGAAGGGGAAGAAGTCCAAGAAGAAGAAAGATAATGAGGAAGCAGACGGTGAGAATGGTGATGAAGCGAAAAATTCTGCTGAGAAAAACCAACAAGCTGATGAAAATGGTGGTGATGATGTTGATGCTGATGATGATGACGGTGAGTCAGACAAAGAGGGTGGGAATCAAAACAACGAAGCTGGTGGAGAACAAAGTGCTGGTGCTGGTGCTTCAACTGGCGCCAAAAAGAAGAAAAAGAAAAAGAAGAAGAAAGCGCAAAACGGGAATCCTCCCAATGGTGGCACTGGCATCCCCAGTGAGCATTTTGGTGATGCACATCGGGCGGGTGCTAATGGGCCGCCTATGGTTGGTCCAGACATTTCTCACCCAATGGCTGCCATGAATCTCGGCCCACCTATCCAACATGTATACCCGGGTCCATACCCGACACCGATGTATTATCAGCCTCCGGCCTCATATGGTCTAAGTTACAGTACGACGTATCCGAGCTCAAGTGCTTCATATTTTACCCCTGCTATGCATGAAAACACCTACTATCACCCGGACATCTACTCGCCCTCTGATCCTGTTGATTCGTTTACGGACGATGATGAAGAAAGTGGCTGCTACATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

290

Amino Acids

31.03

Weight (kDa)

5.75

Isoelectric Point (pI)

29.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 20 - 76 2.2e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011694)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27690
fragaria_vesca FvH4_2g25240 FvH4_3g05420
malus_domestica MD03G1282300.v1.1 MD11G1304000.v1.1
prunus_persica Prupe.8G257200_v2.0.a1
pyrus_communis pycom03g22310 pycom11g26560
rosa_chinensis RchiOBHm_Chr6g0293381
rosa_laevigata RLG00000011951
rosa_multiflora Rmu_sc0006269.1_g000028
rosa_roxburghii Rroxscaffold_7G00174210
rosa_rugosa Rorug06G0235000 Rorug06G0235100
rosa_samantha Rh6AG347900 Rh6BG355500 Rh6CG361600 Rh6DG348700
rosa_wichuraiana Rw6G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 470
AccI GTMKAC 1 cut(s) 660
AciI CCGC 2 cut(s) 575, 590
AclWI GGATC 3 cut(s) 27, 40, 809
AcoI YGGCCR 1 cut(s) 239
AcsI RAATTY 1 cut(s) 322
AcuI CTGAAG 1 cut(s) 39
AcyI GRCGYC 1 cut(s) 471
AfaI GTAC 1 cut(s) 727
AfiI CCNNNNNNNGG 6 cut(s) 52, 237, 254, 527, 530, 599
AflIII ACRYGT 1 cut(s) 655
AgsI TTSAA 6 cut(s) 40, 61, 113, 122, 214, 465
AhdI GACNNNNNGTC 1 cut(s) 394
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 6 cut(s) 17, 208, 235, 347, 431, 741
AluI AGCT 6 cut(s) 17, 208, 235, 347, 431, 741
Alw21I GWGCWC 2 cut(s) 237, 743
Alw26I GTCTC 2 cut(s) 70, 185
AlwI GGATC 3 cut(s) 27, 40, 809
AlwNI CAGNNNCTG 1 cut(s) 173
Ama87I CYCGRG 1 cut(s) 664
AoxI GGCC 4 cut(s) 239, 587, 640, 702
ApeKI GCWGC 4 cut(s) 84, 154, 626, 855
ApoI RAATTY 1 cut(s) 322
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AspLEI GCGC 2 cut(s) 473, 508
AspS9I GGNCC 4 cut(s) 587, 602, 641, 668
AsuC2I CCSGG 3 cut(s) 665, 666, 794
AsuHPI GGTGA 7 cut(s) 311, 320, 374, 404, 572, 608, 782
AvaI CYCGRG 1 cut(s) 664
AvaII GGWCC 2 cut(s) 602, 668
BalI TGGCCA 1 cut(s) 241
BamHI GGATCC 1 cut(s) 32
BanI GGYRCC 1 cut(s) 470
BanII GRGCYC 2 cut(s) 237, 743
Bbv12I GWGCWC 2 cut(s) 237, 743
BbvI GCAGC 4 cut(s) 71, 166, 613, 842
BciT130I CCWGG 1 cut(s) 30
BciVI GTATCC 1 cut(s) 744
BcnI CCSGG 3 cut(s) 665, 666, 794
BcoDI GTCTC 2 cut(s) 70, 185
BfoI RGCGCY 1 cut(s) 474
BfuI GTATCC 1 cut(s) 744
BisI GCNGC 5 cut(s) 85, 155, 590, 627, 856
BlsI GCNGC 5 cut(s) 86, 156, 591, 628, 857
Bme1390I CCNGG 4 cut(s) 30, 665, 666, 794
Bme18I GGWCC 2 cut(s) 602, 668
BmeRI GACNNNNNGTC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 664
BmgT120I GGNCC 4 cut(s) 587, 602, 641, 668
BmiI GGNNCC 3 cut(s) 34, 472, 669
BmrFI CCNGG 4 cut(s) 30, 665, 666, 794
BmrI ACTGGG 1 cut(s) 540
BmsI GCATC 3 cut(s) 364, 549, 553
BmuI ACTGGG 1 cut(s) 540
BpuEI CTTGAG 2 cut(s) 185, 727
BpuMI CCSGG 3 cut(s) 665, 666, 794
BsaBI GATNNNNATC 1 cut(s) 567
BsaHI GRCGYC 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 664
Bsc4I CCNNNNNNNGG 6 cut(s) 52, 237, 254, 527, 530, 599
Bse1I ACTGG 4 cut(s) 178, 472, 541, 546
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 664
BseGI GGATG 1 cut(s) 540
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 6 cut(s) 52, 237, 254, 527, 530, 599
BseMII CTCAG 2 cut(s) 164, 321
BseNI ACTGG 4 cut(s) 178, 472, 541, 546
BseXI GCAGC 4 cut(s) 71, 166, 613, 842
BshFI GGCC 4 cut(s) 241, 589, 642, 704
BshNI GGYRCC 1 cut(s) 470
BsiHKAI GWGCWC 2 cut(s) 237, 743
BsiHKCI CYCGRG 1 cut(s) 664
BsiSI CCGG 3 cut(s) 665, 701, 794
BslI CCNNNNNNNGG 6 cut(s) 52, 237, 254, 527, 530, 599
BsmAI GTCTC 2 cut(s) 70, 185
BsnI GGCC 4 cut(s) 241, 589, 642, 704
Bso31I GGTCTC 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 664
Bsp1286I GDGCHC 2 cut(s) 237, 743
Bsp143I GATC 2 cut(s) 32, 814
BspACI CCGC 2 cut(s) 575, 590
BspANI GGCC 4 cut(s) 241, 589, 642, 704
BspCNI CTCAG 2 cut(s) 163, 322
BspLI GGNNCC 3 cut(s) 34, 472, 669
BspPI GGATC 3 cut(s) 27, 40, 809
BspT107I GGYRCC 1 cut(s) 470
BspTNI GGTCTC 1 cut(s) 185
BsrI ACTGG 4 cut(s) 178, 472, 541, 546
BssECI CCNNGG 1 cut(s) 664
BssMI GATC 2 cut(s) 32, 814
BssNAI GTATAC 1 cut(s) 661
BssNI GRCGYC 1 cut(s) 471
Bst1107I GTATAC 1 cut(s) 661
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 4 cut(s) 169, 299, 392, 725
BstACI GRCGYC 1 cut(s) 471
BstC8I GCNNGC 2 cut(s) 224, 228
BstDEI CTNAG 4 cut(s) 150, 249, 330, 716
BstF5I GGATG 1 cut(s) 540
BstH2I RGCGCY 1 cut(s) 474
BstHHI GCGC 2 cut(s) 473, 508
BstKTI GATC 2 cut(s) 35, 817
BstMAI GTCTC 2 cut(s) 70, 185
BstMBI GATC 2 cut(s) 32, 814
BstMWI GCNNNNNNNGC 2 cut(s) 232, 572
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 659
BstSCI CCNGG 4 cut(s) 28, 663, 664, 792
BstV1I GCAGC 4 cut(s) 71, 166, 613, 842
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
BstZ17I GTATAC 1 cut(s) 661
BsuI GTATCC 1 cut(s) 744
BsuRI GGCC 4 cut(s) 241, 589, 642, 704
BtsCI GGATG 1 cut(s) 540
BtsIMutI CAGTG 3 cut(s) 174, 534, 553
Cac8I GCNNGC 2 cut(s) 224, 228
CaiI CAGNNNCTG 1 cut(s) 173
CfoI GCGC 2 cut(s) 473, 508
Cfr13I GGNCC 4 cut(s) 587, 602, 641, 668
Cfr9I CCCGGG 1 cut(s) 664
Csp6I GTAC 1 cut(s) 726
CspCI CAANNNNNGTGG 2 cut(s) 391, 426
CviAII CATG 4 cut(s) 51, 631, 656, 773
CviQI GTAC 1 cut(s) 726
DdeI CTNAG 4 cut(s) 150, 249, 330, 716
DinI GGCGCC 1 cut(s) 472
DpnI GATC 2 cut(s) 34, 816
DpnII GATC 2 cut(s) 32, 814
DriI GACNNNNNGTC 1 cut(s) 394
EaeI YGGCCR 1 cut(s) 239
Eam1105I GACNNNNNGTC 1 cut(s) 394
Ecl136II GAGCTC 2 cut(s) 235, 741
Eco24I GRGCYC 2 cut(s) 237, 743
Eco31I GGTCTC 1 cut(s) 185
Eco47I GGWCC 2 cut(s) 602, 668
Eco53kI GAGCTC 2 cut(s) 235, 741
Eco57I CTGAAG 1 cut(s) 39
Eco88I CYCGRG 1 cut(s) 664
EcoICRI GAGCTC 2 cut(s) 235, 741
EcoRII CCWGG 1 cut(s) 28
EcoT22I ATGCAT 1 cut(s) 774
EcoT38I GRGCYC 2 cut(s) 237, 743
EgeI GGCGCC 1 cut(s) 472
EheI GGCGCC 1 cut(s) 472
FaeI CATG 4 cut(s) 54, 634, 659, 776
FatI CATG 4 cut(s) 50, 630, 655, 772
FauI CCCGC 1 cut(s) 568
FauNDI CATATG 1 cut(s) 709
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 5 cut(s) 85, 155, 590, 627, 856
FokI GGATG 1 cut(s) 527
FriOI GRGCYC 2 cut(s) 237, 743
Fsp4HI GCNGC 5 cut(s) 85, 155, 590, 627, 856
GlaI GCGC 2 cut(s) 472, 507
GluI GCNGC 5 cut(s) 85, 155, 590, 627, 856
HaeII RGCGCY 1 cut(s) 474
HaeIII GGCC 4 cut(s) 241, 589, 642, 704
HapII CCGG 3 cut(s) 665, 701, 794
HhaI GCGC 2 cut(s) 473, 508
Hin1I GRCGYC 1 cut(s) 471
Hin1II CATG 4 cut(s) 54, 634, 659, 776
Hin6I GCGC 2 cut(s) 471, 506
HinP1I GCGC 2 cut(s) 471, 506
HinfI GANTC 7 cut(s) 70, 146, 395, 415, 517, 634, 824
HpaII CCGG 3 cut(s) 665, 701, 794
HphI GGTGA 7 cut(s) 311, 320, 374, 404, 572, 608, 782
Hpy166II GTNNAC 3 cut(s) 130, 661, 831
Hpy188I TCNGA 3 cut(s) 400, 738, 814
Hpy188III TCNNGA 4 cut(s) 67, 100, 202, 605
Hpy8I GTNNAC 3 cut(s) 130, 661, 831
Hpy99I CGWCG 1 cut(s) 733
HpyAV CCTTC 3 cut(s) 74, 116, 247
HpyCH4III ACNGT 4 cut(s) 169, 299, 392, 725
HpyCH4IV ACGT 2 cut(s) 180, 731
HpyCH4V TGCA 3 cut(s) 87, 566, 772
HpyF10VI GCNNNNNNNGC 2 cut(s) 232, 572
HpyF3I CTNAG 4 cut(s) 150, 249, 330, 716
HpySE526I ACGT 2 cut(s) 180, 731
Hsp92I GRCGYC 1 cut(s) 471
Hsp92II CATG 4 cut(s) 54, 634, 659, 776
HspAI GCGC 2 cut(s) 471, 506
KasI GGCGCC 1 cut(s) 470
Kzo9I GATC 2 cut(s) 32, 814
Lsp1109I GCAGC 4 cut(s) 71, 166, 613, 842
LweI GCATC 3 cut(s) 364, 549, 553
MaeII ACGT 2 cut(s) 180, 731
MaeIII GTNAC 3 cut(s) 163, 169, 719
MalI GATC 2 cut(s) 34, 816
MboI GATC 2 cut(s) 32, 814
MboII GAAGA 9 cut(s) 32, 101, 271, 283, 286, 493, 508, 511, 857
MflI RGATCY 1 cut(s) 32
MhlI GDGCHC 2 cut(s) 237, 743
MlsI TGGCCA 1 cut(s) 241
MluCI AATT 1 cut(s) 322
MluNI TGGCCA 1 cut(s) 241
Mly113I GGCGCC 1 cut(s) 471
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 676
MnlI CCTC 7 cut(s) 36, 280, 400, 531, 708, 715, 820
Mox20I TGGCCA 1 cut(s) 241
Mph1103I ATGCAT 1 cut(s) 774
MscI TGGCCA 1 cut(s) 241
MslI CAYNNNNRTG 1 cut(s) 558
Msp20I TGGCCA 1 cut(s) 241
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 3 cut(s) 665, 701, 794
MspR9I CCNGG 4 cut(s) 30, 665, 666, 794
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 2 cut(s) 232, 572
NarI GGCGCC 1 cut(s) 471
NciI CCSGG 3 cut(s) 665, 666, 794
NdeI CATATG 1 cut(s) 709
NdeII GATC 2 cut(s) 32, 814
NlaIII CATG 4 cut(s) 54, 634, 659, 776
NlaIV GGNNCC 3 cut(s) 34, 472, 669
NmeAIII GCCGAG 2 cut(s) 256, 618
NmuCI GTSAC 2 cut(s) 163, 169
NsiI ATGCAT 1 cut(s) 774
NspI RCATGY 1 cut(s) 659
PciI ACATGT 1 cut(s) 655
PcsI WCGNNNNNNNCGW 1 cut(s) 734
PfeI GAWTC 6 cut(s) 70, 146, 415, 517, 634, 824
PfoI TCCNGGA 1 cut(s) 28
PkrI GCNGC 5 cut(s) 86, 156, 591, 628, 857
PleI GAGTC 1 cut(s) 403
PluTI GGCGCC 1 cut(s) 474
PpsI GAGTC 1 cut(s) 403
PscI ACATGT 1 cut(s) 655
Psp124BI GAGCTC 2 cut(s) 237, 743
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 3 cut(s) 34, 472, 669
PspPI GGNCC 4 cut(s) 587, 602, 641, 668
PstNI CAGNNNCTG 1 cut(s) 173
PsuI RGATCY 1 cut(s) 32
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 1 cut(s) 727
RsaNI GTAC 1 cut(s) 726
RseI CAYNNNNRTG 1 cut(s) 558
SacI GAGCTC 2 cut(s) 237, 743
SatI GCNGC 5 cut(s) 85, 155, 590, 627, 856
Sau3AI GATC 2 cut(s) 32, 814
Sau96I GGNCC 4 cut(s) 587, 602, 641, 668
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 4 cut(s) 30, 665, 666, 794
SduI GDGCHC 2 cut(s) 237, 743
SfaNI GCATC 3 cut(s) 364, 549, 553
SfoI GGCGCC 1 cut(s) 472
SinI GGWCC 2 cut(s) 602, 668
SmaI CCCGGG 1 cut(s) 666
SmiMI CAYNNNNRTG 1 cut(s) 558
SmlI CTYRAG 2 cut(s) 200, 742
SmoI CTYRAG 2 cut(s) 200, 742
Sse9I AATT 1 cut(s) 322
SsiI CCGC 2 cut(s) 575, 590
SspDI GGCGCC 1 cut(s) 470
SstI GAGCTC 2 cut(s) 237, 743
StyD4I CCNGG 4 cut(s) 28, 663, 664, 792
TaaI ACNGT 4 cut(s) 169, 299, 392, 725
TaiI ACGT 2 cut(s) 183, 734
TaqI TCGA 1 cut(s) 68
TasI AATT 1 cut(s) 322
TauI GCSGC 1 cut(s) 592
TfiI GAWTC 6 cut(s) 70, 146, 415, 517, 634, 824
TscAI CASTG 3 cut(s) 174, 541, 553
TseFI GTSAC 2 cut(s) 163, 169
TseI GCWGC 4 cut(s) 84, 154, 626, 855
Tsp45I GTSAC 2 cut(s) 163, 169
TspDTI ATGAA 7 cut(s) 62, 327, 366, 647, 741, 789, 858
TspGWI ACGGA 1 cut(s) 848
TspMI CCCGGG 1 cut(s) 664
TspRI CASTG 3 cut(s) 174, 541, 553
VpaK11BI GGWCC 2 cut(s) 602, 668
XapI RAATTY 1 cut(s) 322
XceI RCATGY 1 cut(s) 659
XmaI CCCGGG 1 cut(s) 664
XmiI GTMKAC 1 cut(s) 660
Zsp2I ATGCAT 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.