MD03G1282300.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011694
Physical Location & Seq
Reverse (-)
36297338 .. 36299254
1917 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1282300.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGACCAAACCAGATGCTTTCTTCCCACTCTTTAAACCTCATCACACATTCACTCAAAGCTCCCCACTTTCCTTATTCCCACTTCATCAGTCTCTCTCTCTCTCTCGCTCATCAATGGCAACCAAACCAGCTGAAGAAGCTCCTGGAACCTTAAAATACCAGACATGGGTTTTGAAAGTCTCAATCCACTGTGGAGGATGCCAGAAGAAGGTCAAGAAAGTCCTTCAAAACATTGAAGGGGTTTACACGACGGAGATTGATTCGCAGCAGCACAAGGTGACTGTGACGGGCAACGTGGAGGCGGAGACCCTACTGAAGAAGCTGTTGAAGTCAGGGAAGCAAGCTGAGCTTTGGCCAGAAGTTAAGAAAGCGAAAAAGTCGAAATCAAAGAATAATAGTAATAACAATGAGAAGCAGCCTGAAGATCATAACCATGAACAAGATGGTGATCATGATCAACCGGATAAAACCGACGGCGGTGATGATGCTGACGACGGCGGTGAGTCCGACGCAGAGGGTGGCGAGAGTAATGAAGCTGGTGGAGAAAGTGGTACTAGTCCTGCTGTTGGTGCTGGTGGTGGAAATAGTGCAAGTACTGGGGCCAAAAAGAAGAAGAAGAAGAAGAAGAAAGCGCAAAATGGTAATTCCCCCACCGGCGGTGGTGCCAACCTCGGCGAGAATGCTCCGCCTCCTGTGGGCAGTATTGAGCCGTACATGGCCGGTGGGCCAGAAATGACTCAGCCGATGGCCTCCATGAATCTTGGCCCACCAGTTCAGCATACGTACCCCGGCCCGTACCCAACATCAATGTACTATCCACCTCCAACGTACGGGCTAAGTTACAATACGGCGTACCCTACCAGTACAAGTAGCTCATACTTCGCTCCTGATCCCATGCATACTGCTACGTATTCTCATCCAGGCAGGTTCTCGTTTCCTCTGCCGTCCTATTCTATCGATGATGATGGTGATGAAGATACGTCCGTTAACAAAGAATATGGAAGTGGGTGCTACGTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

341

Amino Acids

36.35

Weight (kDa)

6.01

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 58 - 113 1.3e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011694)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27690
fragaria_vesca FvH4_2g25240 FvH4_3g05420
malus_domestica MD03G1282300.v1.1 MD11G1304000.v1.1
prunus_persica Prupe.8G257200_v2.0.a1
pyrus_communis pycom03g22310 pycom11g26560
rosa_chinensis RchiOBHm_Chr6g0293381
rosa_laevigata RLG00000011951
rosa_multiflora Rmu_sc0006269.1_g000028
rosa_roxburghii Rroxscaffold_7G00174210
rosa_rugosa Rorug06G0235000 Rorug06G0235100
rosa_samantha Rh6AG347900 Rh6BG355500 Rh6CG361600 Rh6DG348700
rosa_wichuraiana Rw6G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 915
AccB1I GGYRCC 1 cut(s) 662
AciI CCGC 5 cut(s) 302, 477, 498, 657, 686
AclWI GGATC 1 cut(s) 884
AcoI YGGCCR 2 cut(s) 353, 717
AcuI CTGAAG 3 cut(s) 153, 335, 441
AdeI CACNNNGTG 1 cut(s) 277
AfaI GTAC 9 cut(s) 553, 595, 713, 785, 797, 812, 830, 854, 865
AfiI CCNNNNNNNGG 6 cut(s) 166, 208, 566, 656, 695, 830
AgsI TTSAA 4 cut(s) 175, 227, 236, 328
AhlI ACTAGT 1 cut(s) 554
AjnI CCWGG 2 cut(s) 142, 919
AluBI AGCT 8 cut(s) 60, 131, 140, 322, 344, 349, 536, 873
AluI AGCT 8 cut(s) 60, 131, 140, 322, 344, 349, 536, 873
Alw26I GTCTC 3 cut(s) 96, 184, 299
AlwI GGATC 1 cut(s) 884
AoxI GGCC 7 cut(s) 353, 600, 717, 725, 747, 763, 790
ApeKI GCWGC 3 cut(s) 265, 268, 415
ArsI GACNNNNNNTTYG 2 cut(s) 154, 186
AspLEI GCGC 1 cut(s) 634
AspS9I GGNCC 4 cut(s) 600, 725, 764, 791
AsuC2I CCSGG 1 cut(s) 789
AsuHPI GGTGA 5 cut(s) 289, 458, 491, 512, 980
BaeI ACNNNNGTAYC 2 cut(s) 969, 1002
BalI TGGCCA 1 cut(s) 355
BanI GGYRCC 1 cut(s) 662
BbvI GCAGC 3 cut(s) 277, 280, 427
BccI CCATC 3 cut(s) 437, 739, 959
BceAI ACGGC 5 cut(s) 490, 511, 694, 864, 928
BciT130I CCWGG 2 cut(s) 144, 921
BclI TGATCA 2 cut(s) 448, 454
BcnI CCSGG 1 cut(s) 789
BcoDI GTCTC 3 cut(s) 96, 184, 299
BcuI ACTAGT 1 cut(s) 554
BfaI CTAG 1 cut(s) 555
BfuAI ACCTGC 1 cut(s) 915
BisI GCNGC 3 cut(s) 266, 269, 416
BlpI GCTNAGC 1 cut(s) 345
BlsI GCNGC 3 cut(s) 267, 270, 417
BmcAI AGTACT 1 cut(s) 595
Bme1390I CCNGG 3 cut(s) 144, 789, 921
BmgT120I GGNCC 4 cut(s) 600, 725, 764, 791
BmiI GGNNCC 3 cut(s) 148, 601, 664
BmrFI CCNGG 3 cut(s) 144, 789, 921
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 3 cut(s) 4, 188, 475
BmuI ACTGGG 1 cut(s) 606
Bpu1102I GCTNAGC 1 cut(s) 345
BpuMI CCSGG 1 cut(s) 789
Bsa29I ATCGAT 1 cut(s) 957
BsaAI YACGTR 2 cut(s) 783, 909
BsaBI GATNNNNATC 2 cut(s) 447, 453
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 2 cut(s) 670, 787
BsaWI WCCGGW 1 cut(s) 460
Bsc4I CCNNNNNNNGG 6 cut(s) 166, 208, 566, 656, 695, 830
Bse118I RCCGGY 2 cut(s) 653, 719
Bse1I ACTGG 3 cut(s) 601, 770, 861
Bse8I GATNNNNATC 2 cut(s) 447, 453
BseBI CCWGG 2 cut(s) 144, 921
BseCI ATCGAT 1 cut(s) 957
BseDI CCNNGG 2 cut(s) 670, 787
BseGI GGATG 2 cut(s) 203, 916
BseJI GATNNNNATC 2 cut(s) 447, 453
BseLI CCNNNNNNNGG 6 cut(s) 166, 208, 566, 656, 695, 830
BseMII CTCAG 2 cut(s) 336, 752
BseNI ACTGG 3 cut(s) 601, 770, 861
BseXI GCAGC 3 cut(s) 277, 280, 427
BshFI GGCC 7 cut(s) 355, 602, 719, 727, 749, 765, 792
BshNI GGYRCC 1 cut(s) 662
BshVI ATCGAT 1 cut(s) 957
BsiSI CCGG 4 cut(s) 461, 654, 720, 789
BsiWI CGTACG 1 cut(s) 828
BslI CCNNNNNNNGG 6 cut(s) 166, 208, 566, 656, 695, 830
BsmAI GTCTC 3 cut(s) 96, 184, 299
BsmI GAATGC 1 cut(s) 685
BsnI GGCC 7 cut(s) 355, 602, 719, 727, 749, 765, 792
Bso31I GGTCTC 1 cut(s) 299
Bsp143I GATC 4 cut(s) 424, 448, 454, 889
Bsp1720I GCTNAGC 1 cut(s) 345
BspACI CCGC 5 cut(s) 302, 477, 498, 657, 686
BspANI GGCC 7 cut(s) 355, 602, 719, 727, 749, 765, 792
BspCNI CTCAG 2 cut(s) 337, 751
BspDI ATCGAT 1 cut(s) 957
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 3 cut(s) 148, 601, 664
BspMI ACCTGC 1 cut(s) 915
BspPI GGATC 1 cut(s) 884
BspT107I GGYRCC 1 cut(s) 662
BspTNI GGTCTC 1 cut(s) 299
BsrFI RCCGGY 2 cut(s) 653, 719
BsrI ACTGG 3 cut(s) 601, 770, 861
BssAI RCCGGY 2 cut(s) 653, 719
BssECI CCNNGG 2 cut(s) 670, 787
BssMI GATC 4 cut(s) 424, 448, 454, 889
Bst2UI CCWGG 2 cut(s) 144, 921
Bst4CI ACNGT 2 cut(s) 191, 283
BstBAI YACGTR 2 cut(s) 783, 909
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 3 cut(s) 345, 738, 836
BstF5I GGATG 2 cut(s) 203, 916
BstHHI GCGC 1 cut(s) 634
BstKTI GATC 4 cut(s) 427, 451, 457, 892
BstMAI GTCTC 3 cut(s) 96, 184, 299
BstMBI GATC 4 cut(s) 424, 448, 454, 889
BstMWI GCNNNNNNNGC 3 cut(s) 137, 346, 569
BstNI CCWGG 2 cut(s) 144, 921
BstSCI CCNGG 3 cut(s) 142, 787, 919
BstSNI TACGTA 2 cut(s) 783, 909
BstV1I GCAGC 3 cut(s) 277, 280, 427
Bsu15I ATCGAT 1 cut(s) 957
BsuRI GGCC 7 cut(s) 355, 602, 719, 727, 749, 765, 792
BsuTUI ATCGAT 1 cut(s) 957
BtsCI GGATG 2 cut(s) 203, 916
BtsIMutI CAGTG 1 cut(s) 187
BveI ACCTGC 1 cut(s) 915
Cac8I GCNNGC 1 cut(s) 342
CciI TCATGA 1 cut(s) 451
CfoI GCGC 1 cut(s) 634
Cfr10I RCCGGY 2 cut(s) 653, 719
Cfr13I GGNCC 4 cut(s) 600, 725, 764, 791
ClaI ATCGAT 1 cut(s) 957
CseI GACGC 1 cut(s) 518
Csp6I GTAC 9 cut(s) 552, 594, 712, 784, 796, 811, 829, 853, 864
CviAII CATG 6 cut(s) 165, 434, 452, 715, 754, 895
CviQI GTAC 9 cut(s) 552, 594, 712, 784, 796, 811, 829, 853, 864
DdeI CTNAG 3 cut(s) 345, 738, 836
DpnI GATC 4 cut(s) 426, 450, 456, 891
DpnII GATC 4 cut(s) 424, 448, 454, 889
DraI TTTAAA 1 cut(s) 34
DraIII CACNNNGTG 1 cut(s) 277
EaeI YGGCCR 2 cut(s) 353, 717
EciI GGCGGA 2 cut(s) 317, 675
Eco105I TACGTA 2 cut(s) 783, 909
Eco31I GGTCTC 1 cut(s) 299
Eco57I CTGAAG 3 cut(s) 153, 335, 441
EcoRII CCWGG 2 cut(s) 142, 919
EcoT22I ATGCAT 1 cut(s) 900
FaeI CATG 6 cut(s) 168, 437, 455, 718, 757, 898
FalI AAGNNNNNCTT 2 cut(s) 333, 365
FatI CATG 6 cut(s) 164, 433, 451, 714, 753, 894
FbaI TGATCA 2 cut(s) 448, 454
Fnu4HI GCNGC 3 cut(s) 266, 269, 416
FokI GGATG 2 cut(s) 210, 903
Fsp4HI GCNGC 3 cut(s) 266, 269, 416
FspBI CTAG 1 cut(s) 555
GlaI GCGC 1 cut(s) 633
GluI GCNGC 3 cut(s) 266, 269, 416
HaeIII GGCC 7 cut(s) 355, 602, 719, 727, 749, 765, 792
HapII CCGG 4 cut(s) 461, 654, 720, 789
HgaI GACGC 1 cut(s) 518
HhaI GCGC 1 cut(s) 634
Hin1II CATG 6 cut(s) 168, 437, 455, 718, 757, 898
Hin6I GCGC 1 cut(s) 632
HinP1I GCGC 1 cut(s) 632
HincII GTYRAC 1 cut(s) 988
HindII GTYRAC 1 cut(s) 988
HinfI GANTC 4 cut(s) 260, 503, 736, 757
HpaI GTTAAC 1 cut(s) 988
HpaII CCGG 4 cut(s) 461, 654, 720, 789
HphI GGTGA 5 cut(s) 289, 458, 491, 512, 980
Hpy166II GTNNAC 2 cut(s) 244, 988
Hpy188I TCNGA 1 cut(s) 508
Hpy188III TCNNGA 3 cut(s) 214, 452, 887
Hpy8I GTNNAC 2 cut(s) 244, 988
Hpy99I CGWCG 4 cut(s) 253, 476, 497, 512
HpyAV CCTTC 3 cut(s) 202, 230, 233
HpyCH4III ACNGT 2 cut(s) 191, 283
HpyCH4IV ACGT 6 cut(s) 294, 782, 827, 908, 980, 1014
HpyCH4V TGCA 2 cut(s) 590, 898
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 346, 569
HpyF3I CTNAG 3 cut(s) 345, 738, 836
HpySE526I ACGT 6 cut(s) 294, 782, 827, 908, 980, 1014
Hsp92II CATG 6 cut(s) 168, 437, 455, 718, 757, 898
HspAI GCGC 1 cut(s) 632
Ksp22I TGATCA 2 cut(s) 448, 454
KspAI GTTAAC 1 cut(s) 988
Kzo9I GATC 4 cut(s) 424, 448, 454, 889
LmnI GCTCC 4 cut(s) 65, 145, 688, 889
Lsp1109I GCAGC 3 cut(s) 277, 280, 427
LweI GCATC 3 cut(s) 4, 188, 475
MaeI CTAG 1 cut(s) 555
MaeII ACGT 6 cut(s) 294, 782, 827, 908, 980, 1014
MaeIII GTNAC 3 cut(s) 277, 283, 839
MalI GATC 4 cut(s) 426, 450, 456, 891
MboI GATC 4 cut(s) 424, 448, 454, 889
MlsI TGGCCA 1 cut(s) 355
MluCI AATT 1 cut(s) 643
MluNI TGGCCA 1 cut(s) 355
MlyI GAGTC 2 cut(s) 512, 730
MmeI TCCRAC 2 cut(s) 531, 848
MnlI CCTC 9 cut(s) 48, 188, 292, 508, 680, 699, 760, 831, 948
Mox20I TGGCCA 1 cut(s) 355
Mph1103I ATGCAT 1 cut(s) 900
MscI TGGCCA 1 cut(s) 355
MseI TTAA 4 cut(s) 33, 152, 363, 987
MslI CAYNNNNRTG 1 cut(s) 432
Msp20I TGGCCA 1 cut(s) 355
MspA1I CMGCKG 1 cut(s) 131
MspI CCGG 4 cut(s) 461, 654, 720, 789
MspR9I CCNGG 3 cut(s) 144, 789, 921
Mva1269I GAATGC 1 cut(s) 685
MvaI CCWGG 2 cut(s) 144, 921
MwoI GCNNNNNNNGC 3 cut(s) 137, 346, 569
NciI CCSGG 1 cut(s) 789
NdeII GATC 4 cut(s) 424, 448, 454, 889
NlaIII CATG 6 cut(s) 168, 437, 455, 718, 757, 898
NlaIV GGNNCC 3 cut(s) 148, 601, 664
NmeAIII GCCGAG 1 cut(s) 651
NmuCI GTSAC 2 cut(s) 277, 283
NsiI ATGCAT 1 cut(s) 900
PagI TCATGA 1 cut(s) 451
PctI GAATGC 1 cut(s) 685
PfeI GAWTC 2 cut(s) 260, 757
Pfl23II CGTACG 1 cut(s) 828
PfoI TCCNGGA 1 cut(s) 142
PkrI GCNGC 3 cut(s) 267, 270, 417
PleI GAGTC 2 cut(s) 511, 730
PpsI GAGTC 2 cut(s) 511, 730
Ppu21I YACGTR 2 cut(s) 783, 909
Psp6I CCWGG 2 cut(s) 142, 919
PspGI CCWGG 2 cut(s) 142, 919
PspLI CGTACG 1 cut(s) 828
PspN4I GGNNCC 3 cut(s) 148, 601, 664
PspPI GGNCC 4 cut(s) 600, 725, 764, 791
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 9 cut(s) 553, 595, 713, 785, 797, 812, 830, 854, 865
RsaNI GTAC 9 cut(s) 552, 594, 712, 784, 796, 811, 829, 853, 864
RseI CAYNNNNRTG 1 cut(s) 432
SaqAI TTAA 4 cut(s) 33, 152, 363, 987
SatI GCNGC 3 cut(s) 266, 269, 416
Sau3AI GATC 4 cut(s) 424, 448, 454, 889
Sau96I GGNCC 4 cut(s) 600, 725, 764, 791
ScaI AGTACT 1 cut(s) 595
SchI GAGTC 2 cut(s) 512, 730
ScrFI CCNGG 3 cut(s) 144, 789, 921
SfaNI GCATC 3 cut(s) 4, 188, 475
SgrAI CRCCGGYG 1 cut(s) 653
SmiMI CAYNNNNRTG 1 cut(s) 432
SnaBI TACGTA 2 cut(s) 783, 909
SpeI ACTAGT 1 cut(s) 554
Sse9I AATT 1 cut(s) 643
SsiI CCGC 5 cut(s) 302, 477, 498, 657, 686
SspMI CTAG 1 cut(s) 555
StyD4I CCNGG 3 cut(s) 142, 787, 919
TaaI ACNGT 2 cut(s) 191, 283
TaiI ACGT 6 cut(s) 297, 785, 830, 911, 983, 1017
TaqI TCGA 2 cut(s) 380, 957
TasI AATT 1 cut(s) 643
TatI WGTACW 3 cut(s) 593, 810, 863
TfiI GAWTC 2 cut(s) 260, 757
Tru1I TTAA 4 cut(s) 33, 152, 363, 987
Tru9I TTAA 4 cut(s) 33, 152, 363, 987
TscAI CASTG 1 cut(s) 194
TseFI GTSAC 2 cut(s) 277, 283
TseI GCWGC 3 cut(s) 265, 268, 415
Tsp45I GTSAC 2 cut(s) 277, 283
TspDTI ATGAA 5 cut(s) 74, 450, 546, 770, 987
TspGWI ACGGA 2 cut(s) 266, 973
TspRI CASTG 1 cut(s) 194
XcmI CCANNNNNNNNNTGG 1 cut(s) 440
XspI CTAG 1 cut(s) 555
ZrmI AGTACT 1 cut(s) 595
Zsp2I ATGCAT 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.