Rorug06G0235100

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011694
Physical Location & Seq
Reverse (-)
37774839 .. 37776406
1568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0235100.1

Sequence Viewer

Length: 990 bp
ATGGCGTTCTCAGTGGTTTCGACTCCGGCGCATTTCCACCTACCGAAAAAGCTTACTTTCCGAATTCGGTGCTGCGCGGCTTCATCGGTTTCGACCCGATCAAAGTCGACCCGGTTCGATTTGAAAACCTACTGGACGACGCTGATCGCCGATATCAACCACAAGCTTAACGAGGCGGTTCCGGTTCGGTATCCTGAGCTGATCTACGAGTCCATGCGCTACTCCGTTCTCGCTGACGGCGCCAAGAGGGCTTCTCCGGTCATGTGCGTCGCCGCCTGTGAGCTCTTCGGCGGTGACCGCCTCGCCGCCTTCCCCACCGCCTGTGCTCTCGAAATGGTACATGCAGCTTCACTCATACACGATGATCTCCCTTGCATGGATGACGACCCGTCTCGTCGCGGGCAGCCTTCTAACCACACAGTCTACGGCGAAGACATGGCAATTCTTGCTGGTGATGCTCTGTTTCCTTTAGGATTTCAGCACATTGTGTCAAACACGCCTTCAGATCTTGTGCCAGAGGCTCGACTTCTGCGTGTGATAGCGGAGATTGCCAGAACTGTTGGGTCCACCGGCATGGCGGCTGGTCAGTTCCTTGACCTGGAAGGAGGGCCTAATGCGGTTGAATTCGTGCAGGAGAAGAAGTTTGGGGAAATGGGTGAGTGTTCTGCTGTCTGCGGAGGACTGCTAGCTGGTGCCGAAGATGAGGAGGTAGACAGACTGAGGAGGTACGGGAGAGCTGTTGGGGTTTTGTACCAAGTGGTTGATGATATTCTTGAAGAGAAGAAGAATGGTAAGGATGAGAATGAGAAGAAGGAGAAGAAAGGGAAGAGCTATGTGAAAGTTTATGGGGTTGAAAAGGCTATGGAGGTAGCAGAGAAGCTTAGATCCCAAGCTAAGCAGGAGTTAAATGGTTTTGAGAAGTATGGTGATGGTGCGGTGCCACTTCATAGCTTTGTGGATTATGCAGTTGATAGAAGTTTTAGTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

329

Amino Acids

36.1

Weight (kDa)

5.71

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
polyprenyl_synt PF00348 66 - 285 9.4e-35 Polyprenyl synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011694)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27690
fragaria_vesca FvH4_2g25240 FvH4_3g05420
malus_domestica MD03G1282300.v1.1 MD11G1304000.v1.1
prunus_persica Prupe.8G257200_v2.0.a1
pyrus_communis pycom03g22310 pycom11g26560
rosa_chinensis RchiOBHm_Chr6g0293381
rosa_laevigata RLG00000011951
rosa_multiflora Rmu_sc0006269.1_g000028
rosa_roxburghii Rroxscaffold_7G00174210
rosa_rugosa Rorug06G0235000 Rorug06G0235100
rosa_samantha Rh6AG347900 Rh6BG355500 Rh6CG361600 Rh6DG348700
rosa_wichuraiana Rw6G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 239, 692, 937
AccI GTMKAC 3 cut(s) 107, 423, 711
AccII CGCG 2 cut(s) 77, 399
AclWI GGATC 1 cut(s) 879
AcsI RAATTY 2 cut(s) 63, 623
AcuI CTGAAG 1 cut(s) 486
AcyI GRCGYC 1 cut(s) 240
AdeI CACNNNGTG 1 cut(s) 487
AfaI GTAC 3 cut(s) 339, 728, 752
AfiI CCNNNNNNNGG 2 cut(s) 376, 598
AgsI TTSAA 4 cut(s) 124, 623, 776, 854
AhdI GACNNNNNGTC 1 cut(s) 388
AjnI CCWGG 1 cut(s) 597
Alw21I GWGCWC 2 cut(s) 285, 328
Alw26I GTCTC 1 cut(s) 396
AlwI GGATC 1 cut(s) 879
AoxI GGCC 1 cut(s) 608
ApeKI GCWGC 3 cut(s) 72, 344, 403
ApoI RAATTY 2 cut(s) 63, 623
AspLEI GCGC 4 cut(s) 31, 77, 219, 242
AspS9I GGNCC 2 cut(s) 564, 608
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 4 cut(s) 305, 464, 668, 938
AsuNHI GCTAGC 1 cut(s) 685
AvaII GGWCC 1 cut(s) 564
BanI GGYRCC 3 cut(s) 239, 692, 937
BanII GRGCYC 1 cut(s) 285
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 2 cut(s) 285, 328
BbvI GCAGC 3 cut(s) 59, 356, 415
BccI CCATC 1 cut(s) 923
BceAI ACGGC 2 cut(s) 253, 442
BcgI CGANNNNNNTGC 2 cut(s) 51, 85
BciT130I CCWGG 1 cut(s) 599
BciVI GTATCC 1 cut(s) 201
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 396
BfaI CTAG 1 cut(s) 686
BfoI RGCGCY 1 cut(s) 243
BfuI GTATCC 1 cut(s) 201
BglI GCCNNNNNGGC 1 cut(s) 248
BglII AGATCT 1 cut(s) 505
BisI GCNGC 7 cut(s) 73, 78, 273, 306, 345, 404, 579
BlpI GCTNAGC 1 cut(s) 894
BlsI GCNGC 7 cut(s) 74, 79, 274, 307, 346, 405, 580
Bme1390I CCNGG 2 cut(s) 112, 599
Bme18I GGWCC 1 cut(s) 564
BmeRI GACNNNNNGTC 1 cut(s) 388
BmgT120I GGNCC 2 cut(s) 564, 608
BmiI GGNNCC 5 cut(s) 180, 241, 565, 694, 939
BmrFI CCNGG 2 cut(s) 112, 599
BmsI GCATC 1 cut(s) 445
BmtI GCTAGC 1 cut(s) 689
BpiI GAAGAC 1 cut(s) 438
BplI GAGNNNNNCTC 2 cut(s) 238, 270
Bpu10I CCTNAGC 1 cut(s) 195
Bpu1102I GCTNAGC 1 cut(s) 894
BpuMI CCSGG 1 cut(s) 112
BsaHI GRCGYC 1 cut(s) 240
BsaWI WCCGGW 2 cut(s) 181, 256
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 2 cut(s) 376, 598
Bse118I RCCGGY 1 cut(s) 569
Bse1I ACTGG 1 cut(s) 137
BseBI CCWGG 1 cut(s) 599
BseGI GGATG 2 cut(s) 385, 802
BseLI CCNNNNNNNGG 2 cut(s) 376, 598
BseMII CTCAG 3 cut(s) 24, 186, 710
BseNI ACTGG 1 cut(s) 137
BseRI GAGGAG 2 cut(s) 719, 736
BseXI GCAGC 3 cut(s) 59, 356, 415
BsgI GTGCAG 1 cut(s) 650
Bsh1236I CGCG 2 cut(s) 77, 399
BshFI GGCC 1 cut(s) 610
BshNI GGYRCC 3 cut(s) 239, 692, 937
BsiHKAI GWGCWC 2 cut(s) 285, 328
BsiSI CCGG 5 cut(s) 26, 112, 182, 257, 570
BslI CCNNNNNNNGG 2 cut(s) 376, 598
BsmAI GTCTC 1 cut(s) 396
BsmBI CGTCTC 1 cut(s) 396
BsnI GGCC 1 cut(s) 610
Bsp1286I GDGCHC 2 cut(s) 285, 328
Bsp143I GATC 6 cut(s) 98, 144, 201, 364, 505, 884
Bsp1720I GCTNAGC 1 cut(s) 894
BspANI GGCC 1 cut(s) 610
BspCNI CTCAG 3 cut(s) 23, 187, 711
BspFNI CGCG 2 cut(s) 77, 399
BspLI GGNNCC 5 cut(s) 180, 241, 565, 694, 939
BspOI GCTAGC 1 cut(s) 689
BspPI GGATC 1 cut(s) 879
BspQI GCTCTTC 2 cut(s) 290, 821
BspT107I GGYRCC 3 cut(s) 239, 692, 937
BsrFI RCCGGY 1 cut(s) 569
BsrI ACTGG 1 cut(s) 137
BssAI RCCGGY 1 cut(s) 569
BssMI GATC 6 cut(s) 98, 144, 201, 364, 505, 884
BssNI GRCGYC 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 599
Bst4CI ACNGT 2 cut(s) 421, 559
Bst6I CTCTTC 3 cut(s) 290, 771, 821
BstACI GRCGYC 1 cut(s) 240
BstAPI GCANNNNNTGC 1 cut(s) 446
BstC8I GCNNGC 2 cut(s) 401, 687
BstDEI CTNAG 5 cut(s) 10, 195, 719, 881, 894
BstEII GGTNACC 1 cut(s) 293
BstF5I GGATG 2 cut(s) 385, 802
BstFNI CGCG 2 cut(s) 77, 399
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 4 cut(s) 31, 77, 219, 242
BstKTI GATC 6 cut(s) 101, 147, 204, 367, 508, 887
BstMAI GTCTC 1 cut(s) 396
BstMBI GATC 6 cut(s) 98, 144, 201, 364, 505, 884
BstMWI GCNNNNNNNGC 6 cut(s) 239, 248, 297, 446, 455, 548
BstNI CCWGG 1 cut(s) 599
BstNSI RCATGY 1 cut(s) 344
BstPI GGTNACC 1 cut(s) 293
BstSCI CCNGG 2 cut(s) 110, 597
BstUI CGCG 2 cut(s) 77, 399
BstV1I GCAGC 3 cut(s) 59, 356, 415
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 2 cut(s) 505, 884
BstXI CCANNNNNNTGG 1 cut(s) 574
BstYI RGATCY 2 cut(s) 505, 884
BsuI GTATCC 1 cut(s) 201
BsuRI GGCC 1 cut(s) 610
BtsCI GGATG 2 cut(s) 385, 802
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 2 cut(s) 401, 687
CfoI GCGC 4 cut(s) 31, 77, 219, 242
Cfr10I RCCGGY 1 cut(s) 569
Cfr13I GGNCC 2 cut(s) 564, 608
CseI GACGC 2 cut(s) 148, 256
Csp6I GTAC 3 cut(s) 338, 727, 751
CviAII CATG 6 cut(s) 214, 262, 341, 376, 436, 574
CviQI GTAC 3 cut(s) 338, 727, 751
DdeI CTNAG 5 cut(s) 10, 195, 719, 881, 894
DinI GGCGCC 1 cut(s) 241
DpnI GATC 6 cut(s) 100, 146, 203, 366, 507, 886
DpnII GATC 6 cut(s) 98, 144, 201, 364, 505, 884
DraIII CACNNNGTG 1 cut(s) 487
DriI GACNNNNNGTC 1 cut(s) 388
Eam1104I CTCTTC 3 cut(s) 290, 771, 821
Eam1105I GACNNNNNGTC 1 cut(s) 388
EarI CTCTTC 3 cut(s) 290, 771, 821
Ecl136II GAGCTC 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 285
Eco32I GATATC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 564
Eco53kI GAGCTC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 486
Eco91I GGTNACC 1 cut(s) 293
EcoICRI GAGCTC 1 cut(s) 283
EcoO109I RGGNCCY 1 cut(s) 608
EcoO65I GGTNACC 1 cut(s) 293
EcoRI GAATTC 2 cut(s) 63, 623
EcoRII CCWGG 1 cut(s) 597
EcoRV GATATC 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 285
EgeI GGCGCC 1 cut(s) 241
EheI GGCGCC 1 cut(s) 241
Esp3I CGTCTC 1 cut(s) 396
FaeI CATG 6 cut(s) 217, 265, 344, 379, 439, 577
FatI CATG 6 cut(s) 213, 261, 340, 375, 435, 573
FauI CCCGC 1 cut(s) 392
FblI GTMKAC 3 cut(s) 107, 423, 711
Fnu4HI GCNGC 7 cut(s) 73, 78, 273, 306, 345, 404, 579
FokI GGATG 2 cut(s) 392, 809
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 7 cut(s) 73, 78, 273, 306, 345, 404, 579
FspBI CTAG 1 cut(s) 686
GlaI GCGC 4 cut(s) 30, 76, 218, 241
GluI GCNGC 7 cut(s) 73, 78, 273, 306, 345, 404, 579
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 1 cut(s) 610
HapII CCGG 5 cut(s) 26, 112, 182, 257, 570
HgaI GACGC 2 cut(s) 148, 256
HhaI GCGC 4 cut(s) 31, 77, 219, 242
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 6 cut(s) 217, 265, 344, 379, 439, 577
Hin6I GCGC 4 cut(s) 29, 75, 217, 240
HinP1I GCGC 4 cut(s) 29, 75, 217, 240
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
HindIII AAGCTT 3 cut(s) 50, 164, 878
HinfI GANTC 2 cut(s) 22, 209
HpaII CCGG 5 cut(s) 26, 112, 182, 257, 570
HphI GGTGA 4 cut(s) 305, 464, 668, 938
Hpy166II GTNNAC 4 cut(s) 108, 424, 567, 712
Hpy188I TCNGA 2 cut(s) 62, 505
Hpy188III TCNNGA 3 cut(s) 194, 329, 773
Hpy8I GTNNAC 4 cut(s) 108, 424, 567, 712
Hpy99I CGWCG 3 cut(s) 142, 272, 399
HpyAV CCTTC 5 cut(s) 319, 417, 510, 596, 805
HpyCH4III ACNGT 2 cut(s) 421, 559
HpyCH4V TGCA 4 cut(s) 344, 375, 631, 965
HpyF10VI GCNNNNNNNGC 6 cut(s) 239, 248, 297, 446, 455, 548
HpyF3I CTNAG 5 cut(s) 10, 195, 719, 881, 894
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 6 cut(s) 217, 265, 344, 379, 439, 577
HspAI GCGC 4 cut(s) 29, 75, 217, 240
KasI GGCGCC 1 cut(s) 239
Kzo9I GATC 6 cut(s) 98, 144, 201, 364, 505, 884
LguI GCTCTTC 2 cut(s) 290, 821
Lsp1109I GCAGC 3 cut(s) 59, 356, 415
LweI GCATC 1 cut(s) 445
MaeI CTAG 1 cut(s) 686
MaeIII GTNAC 1 cut(s) 293
MalI GATC 6 cut(s) 100, 146, 203, 366, 507, 886
MboI GATC 6 cut(s) 98, 144, 201, 364, 505, 884
MflI RGATCY 2 cut(s) 505, 884
MhlI GDGCHC 2 cut(s) 285, 328
MluCI AATT 3 cut(s) 63, 441, 623
Mly113I GGCGCC 1 cut(s) 240
MlyI GAGTC 2 cut(s) 16, 218
MseI TTAA 3 cut(s) 168, 905, 988
MslI CAYNNNNRTG 1 cut(s) 572
MspI CCGG 5 cut(s) 26, 112, 182, 257, 570
MspR9I CCNGG 2 cut(s) 112, 599
MvaI CCWGG 1 cut(s) 599
MvnI CGCG 2 cut(s) 77, 399
MwoI GCNNNNNNNGC 6 cut(s) 239, 248, 297, 446, 455, 548
NarI GGCGCC 1 cut(s) 240
NciI CCSGG 1 cut(s) 112
NdeII GATC 6 cut(s) 98, 144, 201, 364, 505, 884
NheI GCTAGC 1 cut(s) 685
NlaIII CATG 6 cut(s) 217, 265, 344, 379, 439, 577
NlaIV GGNNCC 5 cut(s) 180, 241, 565, 694, 939
NmuCI GTSAC 1 cut(s) 293
NspI RCATGY 1 cut(s) 344
PciSI GCTCTTC 2 cut(s) 290, 821
PcsI WCGNNNNNNNCGW 1 cut(s) 529
PkrI GCNGC 7 cut(s) 74, 79, 274, 307, 346, 405, 580
PleI GAGTC 2 cut(s) 16, 217
PluTI GGCGCC 1 cut(s) 243
PpsI GAGTC 2 cut(s) 16, 217
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 597
PspEI GGTNACC 1 cut(s) 293
PspGI CCWGG 1 cut(s) 597
PspN4I GGNNCC 5 cut(s) 180, 241, 565, 694, 939
PspPI GGNCC 2 cut(s) 564, 608
PsuI RGATCY 2 cut(s) 505, 884
RsaI GTAC 3 cut(s) 339, 728, 752
RsaNI GTAC 3 cut(s) 338, 727, 751
RseI CAYNNNNRTG 1 cut(s) 572
SacI GAGCTC 1 cut(s) 285
SalI GTCGAC 1 cut(s) 106
SapI GCTCTTC 2 cut(s) 290, 821
SaqAI TTAA 3 cut(s) 168, 905, 988
SatI GCNGC 7 cut(s) 73, 78, 273, 306, 345, 404, 579
Sau3AI GATC 6 cut(s) 98, 144, 201, 364, 505, 884
Sau96I GGNCC 2 cut(s) 564, 608
SchI GAGTC 2 cut(s) 16, 218
ScrFI CCNGG 2 cut(s) 112, 599
SduI GDGCHC 2 cut(s) 285, 328
SfaNI GCATC 1 cut(s) 445
SfoI GGCGCC 1 cut(s) 241
SinI GGWCC 1 cut(s) 564
SmiMI CAYNNNNRTG 1 cut(s) 572
Sse9I AATT 3 cut(s) 63, 441, 623
SspDI GGCGCC 1 cut(s) 239
SspMI CTAG 1 cut(s) 686
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 2 cut(s) 110, 597
TaaI ACNGT 2 cut(s) 421, 559
TaqI TCGA 6 cut(s) 20, 92, 107, 117, 330, 523
TasI AATT 3 cut(s) 63, 441, 623
TauI GCSGC 4 cut(s) 80, 275, 308, 581
Tru1I TTAA 3 cut(s) 168, 905, 988
Tru9I TTAA 3 cut(s) 168, 905, 988
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 3 cut(s) 72, 344, 403
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 2 cut(s) 72, 935
TspGWI ACGGA 1 cut(s) 214
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 1 cut(s) 564
XapI RAATTY 2 cut(s) 63, 623
XceI RCATGY 1 cut(s) 344
XmiI GTMKAC 3 cut(s) 107, 423, 711
XspI CTAG 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.