pycom03g22310

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0011694
Physical Location & Seq
Reverse (-)
22448110 .. 22449456
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g22310.2

Sequence Viewer

Length: 1029 bp
ATGCCTTCTTCCCACTCTTTAAACCTCAGCACACATTCCCTCAAAGCTCCCCACCTTCCTTATTCCCACTTCATCAGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCATCAATGGCAACAAAACCAGCTGAAGAAGCTCCTGGAACCTTAAAATACCTGACATGGGTTTTGAAAGTCTCAATCCACTGTGAAGGATGCAGGAAGAAGGTCAAGAAAGTCCTTCAAAACATCGAAGGGGTTTACACGACGGAGATTGATTCGCAGCAGCACAAGGTGACTGTGACGGGCAACGTGGAGGTGGAGACCCTACTGAAGAAGCTGTGGAAGTCAGGGAAGCATGCTGAGCTTTGGCCAGAAGTTAAGAAAGCGAAAAAGTCCAGGAAATCAAAGAATAATAGTAATAACAATGAGAAGCAACCTGAAGATCATAACCATGAACAAGATGGTGATCATGATCAACCGGATAAAACCGACGGCGGTGATGATGCTGACGACGGCGGTGAGTCCGACGCAGAGGGTGGCGAGAGTAATGAAGCTGGTGGAGAAAGTGGTACTAGTCCTACTGTTGGTGCAGGTGGTGGAAATAGTGCAACTACTGGGGCCAAAAAGAAGAAAAAGAAGAAGAAGAAAGCGCAAAATGGTAATTCCCCCACTGGCGGCGGTGCCAACCTCGGCGAGAAAAATTTTGGACATGATCCGCCTCCTGCGGGCAGTATTGAGCCGTATATGGCCGGTGGGCCAGACATGACTCAGCCGATGGCCTCCATGAATCTTGGCCCACCAGTTCAGCATACGTACCCCGGCCCGTACCCAACATCAATGTACTATCCACCTCCAACGTACGGGCTAAGTTACAATACGGCGTACCCTAACAGTACAAGTACCTCATACTTTGCTCCTGATCCCATGCATACCACTACGTATTCTCAGCCGGGCATGTTCCCGTTTCCTCTGCCGTCCTATTCTATCGATAATGATGATGATAAGTTCGTTGACGATGAAAATGGAAGTGGGTGCTACGTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

343

Amino Acids

36.75

Weight (kDa)

6.0

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011694)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27690
fragaria_vesca FvH4_2g25240 FvH4_3g05420
malus_domestica MD03G1282300.v1.1 MD11G1304000.v1.1
prunus_persica Prupe.8G257200_v2.0.a1
pyrus_communis pycom03g22310 pycom11g26560
rosa_chinensis RchiOBHm_Chr6g0293381
rosa_laevigata RLG00000011951
rosa_multiflora Rmu_sc0006269.1_g000028
rosa_roxburghii Rroxscaffold_7G00174210
rosa_rugosa Rorug06G0235000 Rorug06G0235100
rosa_samantha Rh6AG347900 Rh6BG355500 Rh6CG361600 Rh6DG348700
rosa_wichuraiana Rw6G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 563
Acc36I ACCTGC 1 cut(s) 563
AccB1I GGYRCC 1 cut(s) 662
AciI CCGC 6 cut(s) 477, 498, 657, 660, 698, 707
AclWI GGATC 2 cut(s) 689, 896
AcoI YGGCCR 2 cut(s) 350, 729
AcsI RAATTY 1 cut(s) 682
AcuI CTGAAG 3 cut(s) 150, 332, 441
AdeI CACNNNGTG 1 cut(s) 274
AfaI GTAC 8 cut(s) 553, 797, 809, 824, 842, 866, 877, 883
AfiI CCNNNNNNNGG 5 cut(s) 163, 566, 656, 707, 842
AgsI TTSAA 2 cut(s) 172, 224
AhlI ACTAGT 1 cut(s) 554
AjnI CCWGG 2 cut(s) 139, 377
AluBI AGCT 6 cut(s) 47, 128, 137, 319, 346, 536
AluI AGCT 6 cut(s) 47, 128, 137, 319, 346, 536
Alw26I GTCTC 3 cut(s) 83, 181, 296
AlwI GGATC 2 cut(s) 689, 896
AoxI GGCC 7 cut(s) 350, 600, 729, 737, 759, 775, 802
ApeKI GCWGC 2 cut(s) 262, 265
ApoI RAATTY 1 cut(s) 682
ArsI GACNNNNNNTTYG 2 cut(s) 151, 183
AspLEI GCGC 1 cut(s) 634
AspS9I GGNCC 4 cut(s) 600, 737, 776, 803
AsuC2I CCSGG 2 cut(s) 801, 933
AsuHPI GGTGA 4 cut(s) 286, 458, 491, 512
BalI TGGCCA 1 cut(s) 352
BanI GGYRCC 1 cut(s) 662
BbvCI CCTCAGC 1 cut(s) 26
BbvI GCAGC 2 cut(s) 274, 277
BccI CCATC 2 cut(s) 437, 751
BceAI ACGGC 5 cut(s) 490, 511, 706, 876, 940
BciT130I CCWGG 2 cut(s) 141, 379
BclI TGATCA 2 cut(s) 448, 454
BcnI CCSGG 2 cut(s) 801, 933
BcoDI GTCTC 3 cut(s) 83, 181, 296
BcuI ACTAGT 1 cut(s) 554
BfaI CTAG 1 cut(s) 555
BfuAI ACCTGC 1 cut(s) 563
BisI GCNGC 3 cut(s) 263, 266, 658
BlpI GCTNAGC 1 cut(s) 342
BlsI GCNGC 3 cut(s) 264, 267, 659
Bme1390I CCNGG 4 cut(s) 141, 379, 801, 933
BmgT120I GGNCC 4 cut(s) 600, 737, 776, 803
BmiI GGNNCC 3 cut(s) 145, 601, 664
BmrFI CCNGG 4 cut(s) 141, 379, 801, 933
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 2 cut(s) 185, 475
BmuI ACTGGG 1 cut(s) 606
Bpu10I CCTNAGC 1 cut(s) 26
Bpu1102I GCTNAGC 1 cut(s) 342
BpuMI CCSGG 2 cut(s) 801, 933
Bsa29I ATCGAT 1 cut(s) 969
BsaAI YACGTR 2 cut(s) 795, 921
BsaBI GATNNNNATC 2 cut(s) 447, 453
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 2 cut(s) 670, 799
BsaWI WCCGGW 1 cut(s) 460
Bsc4I CCNNNNNNNGG 5 cut(s) 163, 566, 656, 707, 842
Bse118I RCCGGY 1 cut(s) 731
Bse1I ACTGG 3 cut(s) 601, 658, 782
Bse8I GATNNNNATC 2 cut(s) 447, 453
BseBI CCWGG 2 cut(s) 141, 379
BseCI ATCGAT 1 cut(s) 969
BseDI CCNNGG 2 cut(s) 670, 799
BseGI GGATG 1 cut(s) 200
BseJI GATNNNNATC 2 cut(s) 447, 453
BseLI CCNNNNNNNGG 5 cut(s) 163, 566, 656, 707, 842
BseMII CTCAG 4 cut(s) 40, 333, 764, 941
BseNI ACTGG 3 cut(s) 601, 658, 782
BseXI GCAGC 2 cut(s) 274, 277
BsgI GTGCAG 1 cut(s) 591
BshFI GGCC 7 cut(s) 352, 602, 731, 739, 761, 777, 804
BshNI GGYRCC 1 cut(s) 662
BshVI ATCGAT 1 cut(s) 969
BsiSI CCGG 4 cut(s) 461, 732, 801, 932
BsiWI CGTACG 1 cut(s) 840
BslI CCNNNNNNNGG 5 cut(s) 163, 566, 656, 707, 842
BsmAI GTCTC 3 cut(s) 83, 181, 296
BsnI GGCC 7 cut(s) 352, 602, 731, 739, 761, 777, 804
Bso31I GGTCTC 1 cut(s) 296
Bsp143I GATC 5 cut(s) 424, 448, 454, 694, 901
Bsp1720I GCTNAGC 1 cut(s) 342
BspACI CCGC 6 cut(s) 477, 498, 657, 660, 698, 707
BspANI GGCC 7 cut(s) 352, 602, 731, 739, 761, 777, 804
BspCNI CTCAG 4 cut(s) 39, 334, 763, 940
BspDI ATCGAT 1 cut(s) 969
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 3 cut(s) 145, 601, 664
BspMI ACCTGC 1 cut(s) 563
BspPI GGATC 2 cut(s) 689, 896
BspT107I GGYRCC 1 cut(s) 662
BspTNI GGTCTC 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 731
BsrI ACTGG 3 cut(s) 601, 658, 782
BssAI RCCGGY 1 cut(s) 731
BssECI CCNNGG 2 cut(s) 670, 799
BssMI GATC 5 cut(s) 424, 448, 454, 694, 901
Bst2UI CCWGG 2 cut(s) 141, 379
Bst4CI ACNGT 4 cut(s) 188, 280, 565, 875
BstBAI YACGTR 2 cut(s) 795, 921
BstC8I GCNNGC 2 cut(s) 339, 709
BstDEI CTNAG 5 cut(s) 26, 342, 750, 848, 927
BstF5I GGATG 1 cut(s) 200
BstHHI GCGC 1 cut(s) 634
BstKTI GATC 5 cut(s) 427, 451, 457, 697, 904
BstMAI GTCTC 3 cut(s) 83, 181, 296
BstMBI GATC 5 cut(s) 424, 448, 454, 694, 901
BstMWI GCNNNNNNNGC 2 cut(s) 134, 343
BstNI CCWGG 2 cut(s) 141, 379
BstNSI RCATGY 2 cut(s) 341, 940
BstSCI CCNGG 4 cut(s) 139, 377, 799, 931
BstSNI TACGTA 2 cut(s) 795, 921
BstV1I GCAGC 2 cut(s) 274, 277
Bsu15I ATCGAT 1 cut(s) 969
BsuRI GGCC 7 cut(s) 352, 602, 731, 739, 761, 777, 804
BsuTUI ATCGAT 1 cut(s) 969
BtsCI GGATG 1 cut(s) 200
BtsIMutI CAGTG 2 cut(s) 184, 651
BveI ACCTGC 1 cut(s) 563
Cac8I GCNNGC 2 cut(s) 339, 709
CciI TCATGA 1 cut(s) 451
CfoI GCGC 1 cut(s) 634
Cfr10I RCCGGY 1 cut(s) 731
Cfr13I GGNCC 4 cut(s) 600, 737, 776, 803
ClaI ATCGAT 1 cut(s) 969
CseI GACGC 1 cut(s) 518
Csp6I GTAC 8 cut(s) 552, 796, 808, 823, 841, 865, 876, 882
CviAII CATG 9 cut(s) 162, 338, 434, 452, 692, 745, 766, 907, 937
CviQI GTAC 8 cut(s) 552, 796, 808, 823, 841, 865, 876, 882
DdeI CTNAG 5 cut(s) 26, 342, 750, 848, 927
DpnI GATC 5 cut(s) 426, 450, 456, 696, 903
DpnII GATC 5 cut(s) 424, 448, 454, 694, 901
DraI TTTAAA 1 cut(s) 21
DraIII CACNNNGTG 1 cut(s) 274
EaeI YGGCCR 2 cut(s) 350, 729
EciI GGCGGA 1 cut(s) 687
Eco105I TACGTA 2 cut(s) 795, 921
Eco31I GGTCTC 1 cut(s) 296
Eco57I CTGAAG 3 cut(s) 150, 332, 441
EcoRII CCWGG 2 cut(s) 139, 377
EcoT22I ATGCAT 1 cut(s) 912
FaeI CATG 9 cut(s) 165, 341, 437, 455, 695, 748, 769, 910, 940
FatI CATG 9 cut(s) 161, 337, 433, 451, 691, 744, 765, 906, 936
FauI CCCGC 1 cut(s) 700
FbaI TGATCA 2 cut(s) 448, 454
Fnu4HI GCNGC 3 cut(s) 263, 266, 658
FokI GGATG 1 cut(s) 207
Fsp4HI GCNGC 3 cut(s) 263, 266, 658
FspBI CTAG 1 cut(s) 555
GlaI GCGC 1 cut(s) 633
GluI GCNGC 3 cut(s) 263, 266, 658
HaeIII GGCC 7 cut(s) 352, 602, 731, 739, 761, 777, 804
HapII CCGG 4 cut(s) 461, 732, 801, 932
HgaI GACGC 1 cut(s) 518
HhaI GCGC 1 cut(s) 634
Hin1II CATG 9 cut(s) 165, 341, 437, 455, 695, 748, 769, 910, 940
Hin6I GCGC 1 cut(s) 632
HinP1I GCGC 1 cut(s) 632
HincII GTYRAC 1 cut(s) 994
HindII GTYRAC 1 cut(s) 994
HinfI GANTC 4 cut(s) 257, 503, 748, 769
HpaII CCGG 4 cut(s) 461, 732, 801, 932
HphI GGTGA 4 cut(s) 286, 458, 491, 512
Hpy166II GTNNAC 2 cut(s) 241, 994
Hpy188I TCNGA 1 cut(s) 508
Hpy188III TCNNGA 3 cut(s) 211, 452, 899
Hpy8I GTNNAC 2 cut(s) 241, 994
Hpy99I CGWCG 4 cut(s) 250, 476, 497, 512
HpyAV CCTTC 6 cut(s) 15, 65, 185, 199, 227, 230
HpyCH4III ACNGT 4 cut(s) 188, 280, 565, 875
HpyCH4IV ACGT 5 cut(s) 291, 794, 839, 920, 1020
HpyCH4V TGCA 4 cut(s) 198, 572, 590, 910
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 343
HpyF3I CTNAG 5 cut(s) 26, 342, 750, 848, 927
HpySE526I ACGT 5 cut(s) 291, 794, 839, 920, 1020
Hsp92II CATG 9 cut(s) 165, 341, 437, 455, 695, 748, 769, 910, 940
HspAI GCGC 1 cut(s) 632
Ksp22I TGATCA 2 cut(s) 448, 454
Kzo9I GATC 5 cut(s) 424, 448, 454, 694, 901
LmnI GCTCC 3 cut(s) 52, 142, 901
Lsp1109I GCAGC 2 cut(s) 274, 277
LweI GCATC 2 cut(s) 185, 475
MaeI CTAG 1 cut(s) 555
MaeII ACGT 5 cut(s) 291, 794, 839, 920, 1020
MaeIII GTNAC 3 cut(s) 274, 280, 851
MalI GATC 5 cut(s) 426, 450, 456, 696, 903
MboI GATC 5 cut(s) 424, 448, 454, 694, 901
MboII GAAGA 8 cut(s) 143, 214, 325, 434, 622, 631, 634, 637
MlsI TGGCCA 1 cut(s) 352
MluCI AATT 2 cut(s) 643, 682
MluNI TGGCCA 1 cut(s) 352
MlyI GAGTC 2 cut(s) 512, 742
MmeI TCCRAC 2 cut(s) 531, 860
Mox20I TGGCCA 1 cut(s) 352
Mph1103I ATGCAT 1 cut(s) 912
MscI TGGCCA 1 cut(s) 352
MseI TTAA 3 cut(s) 20, 149, 360
MslI CAYNNNNRTG 1 cut(s) 432
Msp20I TGGCCA 1 cut(s) 352
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 4 cut(s) 461, 732, 801, 932
MspR9I CCNGG 4 cut(s) 141, 379, 801, 933
MvaI CCWGG 2 cut(s) 141, 379
MwoI GCNNNNNNNGC 2 cut(s) 134, 343
NciI CCSGG 2 cut(s) 801, 933
NdeII GATC 5 cut(s) 424, 448, 454, 694, 901
NlaIII CATG 9 cut(s) 165, 341, 437, 455, 695, 748, 769, 910, 940
NlaIV GGNNCC 3 cut(s) 145, 601, 664
NmeAIII GCCGAG 1 cut(s) 651
NmuCI GTSAC 2 cut(s) 274, 280
NsiI ATGCAT 1 cut(s) 912
NspI RCATGY 2 cut(s) 341, 940
PaeI GCATGC 1 cut(s) 341
PagI TCATGA 1 cut(s) 451
PaqCI CACCTGC 1 cut(s) 563
PfeI GAWTC 2 cut(s) 257, 769
Pfl23II CGTACG 1 cut(s) 840
PfoI TCCNGGA 2 cut(s) 139, 377
PkrI GCNGC 3 cut(s) 264, 267, 659
PleI GAGTC 2 cut(s) 511, 742
PpsI GAGTC 2 cut(s) 511, 742
Ppu21I YACGTR 2 cut(s) 795, 921
Psp6I CCWGG 2 cut(s) 139, 377
PspGI CCWGG 2 cut(s) 139, 377
PspLI CGTACG 1 cut(s) 840
PspN4I GGNNCC 3 cut(s) 145, 601, 664
PspPI GGNCC 4 cut(s) 600, 737, 776, 803
PvuII CAGCTG 1 cut(s) 128
RsaI GTAC 8 cut(s) 553, 797, 809, 824, 842, 866, 877, 883
RsaNI GTAC 8 cut(s) 552, 796, 808, 823, 841, 865, 876, 882
RseI CAYNNNNRTG 1 cut(s) 432
SaqAI TTAA 3 cut(s) 20, 149, 360
SatI GCNGC 3 cut(s) 263, 266, 658
Sau3AI GATC 5 cut(s) 424, 448, 454, 694, 901
Sau96I GGNCC 4 cut(s) 600, 737, 776, 803
SchI GAGTC 2 cut(s) 512, 742
ScrFI CCNGG 4 cut(s) 141, 379, 801, 933
SfaNI GCATC 2 cut(s) 185, 475
SmiMI CAYNNNNRTG 1 cut(s) 432
SnaBI TACGTA 2 cut(s) 795, 921
SpeI ACTAGT 1 cut(s) 554
SphI GCATGC 1 cut(s) 341
Sse9I AATT 2 cut(s) 643, 682
SsiI CCGC 6 cut(s) 477, 498, 657, 660, 698, 707
SspMI CTAG 1 cut(s) 555
StyD4I CCNGG 4 cut(s) 139, 377, 799, 931
TaaI ACNGT 4 cut(s) 188, 280, 565, 875
TaiI ACGT 5 cut(s) 294, 797, 842, 923, 1023
TaqI TCGA 2 cut(s) 231, 969
TasI AATT 2 cut(s) 643, 682
TatI WGTACW 2 cut(s) 822, 875
TauI GCSGC 1 cut(s) 660
TfiI GAWTC 2 cut(s) 257, 769
Tru1I TTAA 3 cut(s) 20, 149, 360
Tru9I TTAA 3 cut(s) 20, 149, 360
TscAI CASTG 2 cut(s) 191, 658
TseFI GTSAC 2 cut(s) 274, 280
TseI GCWGC 2 cut(s) 262, 265
Tsp45I GTSAC 2 cut(s) 274, 280
TspDTI ATGAA 5 cut(s) 61, 450, 546, 782, 1014
TspGWI ACGGA 1 cut(s) 263
TspRI CASTG 2 cut(s) 191, 658
XapI RAATTY 1 cut(s) 682
XceI RCATGY 2 cut(s) 341, 940
XcmI CCANNNNNNNNNTGG 1 cut(s) 440
XspI CTAG 1 cut(s) 555
Zsp2I ATGCAT 1 cut(s) 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.