FvH4_2g25240

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011694
Physical Location & Seq
Forward (+)
20550423 .. 20552360
1938 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25240.t1

Sequence Viewer

Length: 873 bp
ATGGCAACAAAACCAGCTGAAGAACCTCCTGGATCCTTGAAATACCAGACATGGGTGTTAAAAGTCTCAATTCATTGTGAAGGCTGCAAAAAGAAAGTCAAGAAAGTTCTTCAAAGCATTGAAGGGGTTTACACTATTACGATTGATTCTCAGCTGCACAAGGTCACAGTGACTGGCAACGTGGAGGCAGAGACCTTGCTCAAGAAGCTATTGAGGTCAAACAAGCACGCTGAGCTTTGGCCAGAACCTAAGAAGGGGAAAAAGTCCAAGAAGAACAAACAGAATGGTGAAGCAGATGGTGAGAATGGTGATGAAGCGAAAAACTCTACTGAGAAAAACCAACAAGCTGATGAAAATGGTGGTGATGATGCTGATGATGATGAGGGTGAGTCGGACAAAGAGGGTGGCAATCAAAGCAATGAAGCTGGTGGAGAACAAAGTGTTGGTGCTGCTGGTGGTGGTGGTGGTGCCAAAAAGAAGAAAAAGAAAAAGAAGAAGAAAGGGCTAAATGGGAATCCTCCAAATGGCGGCACAGGCAACCCCGGTGAGCATCACGGTGATGCTCATCCGGCTGGTGCTAATGGGCCGCCGATGGTTGGTCCAGACATGTCTCACCCAATGGCTGCCATGAATCACGGCCCACCGATCCAACATATGTACCCGGGTCCATACCCGCCACCAATGTATTATCAGCCTCCTCCGGCCGCATACGGGTTACGTTACAGTACGACGTATCCAAGCACAAGTGCTTCATATTTTACCCCTGCTATGCATGATAACACGTACTATCAGCCGGACATCTACTTGCCGTTTGATCCTGTCGATTCACATACAGACGATGATGATGAAACAAGCGGCTGCTACATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

291

Amino Acids

31.16

Weight (kDa)

6.01

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 20 - 72 1.8e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011694)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27690
fragaria_vesca FvH4_2g25240 FvH4_3g05420
malus_domestica MD03G1282300.v1.1 MD11G1304000.v1.1
prunus_persica Prupe.8G257200_v2.0.a1
pyrus_communis pycom03g22310 pycom11g26560
rosa_chinensis RchiOBHm_Chr6g0293381
rosa_laevigata RLG00000011951
rosa_multiflora Rmu_sc0006269.1_g000028
rosa_roxburghii Rroxscaffold_7G00174210
rosa_rugosa Rorug06G0235000 Rorug06G0235100
rosa_samantha Rh6AG347900 Rh6BG355500 Rh6CG361600 Rh6DG348700
rosa_wichuraiana Rw6G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 467
AciI CCGC 5 cut(s) 528, 587, 674, 705, 855
AclWI GGATC 4 cut(s) 27, 40, 640, 809
AcoI YGGCCR 2 cut(s) 239, 702
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 3 cut(s) 659, 727, 785
AfiI CCNNNNNNNGG 6 cut(s) 52, 254, 524, 527, 596, 711
AflIII ACRYGT 2 cut(s) 606, 780
AgsI TTSAA 3 cut(s) 40, 113, 122
AjnI CCWGG 1 cut(s) 28
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
AluBI AGCT 6 cut(s) 17, 154, 208, 235, 347, 425
AluI AGCT 6 cut(s) 17, 154, 208, 235, 347, 425
Alw26I GTCTC 3 cut(s) 70, 185, 615
AlwI GGATC 4 cut(s) 27, 40, 640, 809
AlwNI CAGNNNCTG 1 cut(s) 173
Ama87I CYCGRG 1 cut(s) 661
AoxI GGCC 4 cut(s) 239, 584, 637, 702
ApeKI GCWGC 5 cut(s) 84, 154, 449, 623, 858
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AspS9I GGNCC 4 cut(s) 584, 599, 638, 665
AsuC2I CCSGG 3 cut(s) 543, 662, 663
AsuHPI GGTGA 8 cut(s) 299, 311, 320, 374, 398, 557, 569, 605
AvaI CYCGRG 1 cut(s) 661
AvaII GGWCC 2 cut(s) 599, 665
BaeI ACNNNNGTAYC 2 cut(s) 641, 674
BalI TGGCCA 1 cut(s) 241
BamHI GGATCC 1 cut(s) 32
BanI GGYRCC 1 cut(s) 467
BbvI GCAGC 5 cut(s) 71, 141, 436, 610, 845
BccI CCATC 2 cut(s) 290, 586
BceAI ACGGC 2 cut(s) 652, 793
BciT130I CCWGG 1 cut(s) 30
BciVI GTATCC 1 cut(s) 744
BcnI CCSGG 3 cut(s) 543, 662, 663
BcoDI GTCTC 3 cut(s) 70, 185, 615
BfuI GTATCC 1 cut(s) 744
BisI GCNGC 9 cut(s) 85, 155, 450, 529, 587, 624, 705, 856, 859
BlpI GCTNAGC 1 cut(s) 231
BlsI GCNGC 9 cut(s) 86, 156, 451, 530, 588, 625, 706, 857, 860
Bme1390I CCNGG 4 cut(s) 30, 543, 662, 663
Bme18I GGWCC 2 cut(s) 599, 665
BmeT110I CYCGRG 1 cut(s) 661
BmgT120I GGNCC 4 cut(s) 584, 599, 638, 665
BmiI GGNNCC 3 cut(s) 34, 469, 666
BmrFI CCNGG 4 cut(s) 30, 543, 662, 663
BmsI GCATC 3 cut(s) 358, 550, 559
Bpu1102I GCTNAGC 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 3 cut(s) 543, 662, 663
BsaAI YACGTR 1 cut(s) 783
BsaBI GATNNNNATC 1 cut(s) 564
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 2 cut(s) 541, 661
Bsc4I CCNNNNNNNGG 6 cut(s) 52, 254, 524, 527, 596, 711
Bse1I ACTGG 1 cut(s) 178
Bse3DI GCAATG 1 cut(s) 424
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 2 cut(s) 541, 661
BseGI GGATG 1 cut(s) 565
BseJI GATNNNNATC 1 cut(s) 564
BseLI CCNNNNNNNGG 6 cut(s) 52, 254, 524, 527, 596, 711
BseMI GCAATG 1 cut(s) 424
BseMII CTCAG 3 cut(s) 164, 222, 321
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 1 cut(s) 687
BseX3I CGGCCG 1 cut(s) 702
BseXI GCAGC 5 cut(s) 71, 141, 436, 610, 845
BsgI GTGCAG 1 cut(s) 140
Bsh1285I CGRYCG 1 cut(s) 705
BshFI GGCC 4 cut(s) 241, 586, 639, 704
BshNI GGYRCC 1 cut(s) 467
BsiEI CGRYCG 1 cut(s) 705
BsiHKCI CYCGRG 1 cut(s) 661
BsiSI CCGG 5 cut(s) 543, 569, 662, 701, 794
BslI CCNNNNNNNGG 6 cut(s) 52, 254, 524, 527, 596, 711
BsmAI GTCTC 3 cut(s) 70, 185, 615
BsnI GGCC 4 cut(s) 241, 586, 639, 704
Bso31I GGTCTC 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 661
Bsp143I GATC 3 cut(s) 32, 645, 814
Bsp1720I GCTNAGC 1 cut(s) 231
BspACI CCGC 5 cut(s) 528, 587, 674, 705, 855
BspANI GGCC 4 cut(s) 241, 586, 639, 704
BspCNI CTCAG 3 cut(s) 163, 223, 322
BspLI GGNNCC 3 cut(s) 34, 469, 666
BspPI GGATC 4 cut(s) 27, 40, 640, 809
BspT107I GGYRCC 1 cut(s) 467
BspTNI GGTCTC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 424
BsrI ACTGG 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 541, 661
BssMI GATC 3 cut(s) 32, 645, 814
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 3 cut(s) 169, 557, 725
BstBAI YACGTR 1 cut(s) 783
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 4 cut(s) 150, 231, 249, 330
BstF5I GGATG 1 cut(s) 565
BstKTI GATC 3 cut(s) 35, 648, 817
BstMAI GTCTC 3 cut(s) 70, 185, 615
BstMBI GATC 3 cut(s) 32, 645, 814
BstMCI CGRYCG 1 cut(s) 705
BstMWI GCNNNNNNNGC 5 cut(s) 205, 232, 414, 534, 569
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 610
BstSCI CCNGG 4 cut(s) 28, 541, 660, 661
BstV1I GCAGC 5 cut(s) 71, 141, 436, 610, 845
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
BstZI CGGCCG 1 cut(s) 702
BsuI GTATCC 1 cut(s) 744
BsuRI GGCC 4 cut(s) 241, 586, 639, 704
BtsCI GGATG 1 cut(s) 565
BtsIMutI CAGTG 1 cut(s) 174
Cac8I GCNNGC 1 cut(s) 228
CaiI CAGNNNCTG 1 cut(s) 173
Cfr13I GGNCC 4 cut(s) 584, 599, 638, 665
Cfr9I CCCGGG 1 cut(s) 661
Csp6I GTAC 3 cut(s) 658, 726, 784
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviAII CATG 4 cut(s) 51, 607, 628, 773
CviQI GTAC 3 cut(s) 658, 726, 784
DdeI CTNAG 4 cut(s) 150, 231, 249, 330
DpnI GATC 3 cut(s) 34, 647, 816
DpnII GATC 3 cut(s) 32, 645, 814
EaeI YGGCCR 2 cut(s) 239, 702
EagI CGGCCG 1 cut(s) 702
EclXI CGGCCG 1 cut(s) 702
Eco31I GGTCTC 1 cut(s) 185
Eco47I GGWCC 2 cut(s) 599, 665
Eco52I CGGCCG 1 cut(s) 702
Eco57I CTGAAG 1 cut(s) 39
Eco88I CYCGRG 1 cut(s) 661
EcoRII CCWGG 1 cut(s) 28
EcoT22I ATGCAT 1 cut(s) 774
FaeI CATG 4 cut(s) 54, 610, 631, 776
FatI CATG 4 cut(s) 50, 606, 627, 772
FauI CCCGC 1 cut(s) 681
FauNDI CATATG 1 cut(s) 654
Fnu4HI GCNGC 9 cut(s) 85, 155, 450, 529, 587, 624, 705, 856, 859
FokI GGATG 1 cut(s) 552
Fsp4HI GCNGC 9 cut(s) 85, 155, 450, 529, 587, 624, 705, 856, 859
GluI GCNGC 9 cut(s) 85, 155, 450, 529, 587, 624, 705, 856, 859
HaeIII GGCC 4 cut(s) 241, 586, 639, 704
HapII CCGG 5 cut(s) 543, 569, 662, 701, 794
Hin1II CATG 4 cut(s) 54, 610, 631, 776
HinfI GANTC 5 cut(s) 146, 389, 514, 631, 824
HpaII CCGG 5 cut(s) 543, 569, 662, 701, 794
HphI GGTGA 8 cut(s) 299, 311, 320, 374, 398, 557, 569, 605
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 1 cut(s) 394
Hpy188III TCNNGA 3 cut(s) 100, 202, 602
Hpy8I GTNNAC 1 cut(s) 130
Hpy99I CGWCG 1 cut(s) 733
HpyAV CCTTC 3 cut(s) 74, 116, 247
HpyCH4III ACNGT 3 cut(s) 169, 557, 725
HpyCH4IV ACGT 4 cut(s) 180, 718, 731, 782
HpyCH4V TGCA 3 cut(s) 87, 157, 772
HpyF10VI GCNNNNNNNGC 5 cut(s) 205, 232, 414, 534, 569
HpyF3I CTNAG 4 cut(s) 150, 231, 249, 330
HpySE526I ACGT 4 cut(s) 180, 718, 731, 782
Hsp92II CATG 4 cut(s) 54, 610, 631, 776
Kzo9I GATC 3 cut(s) 32, 645, 814
Lsp1109I GCAGC 5 cut(s) 71, 141, 436, 610, 845
LweI GCATC 3 cut(s) 358, 550, 559
MaeII ACGT 4 cut(s) 180, 718, 731, 782
MaeIII GTNAC 4 cut(s) 163, 169, 714, 719
MalI GATC 3 cut(s) 34, 647, 816
MboI GATC 3 cut(s) 32, 645, 814
MboII GAAGA 6 cut(s) 32, 101, 283, 490, 505, 508
MflI RGATCY 1 cut(s) 32
MlsI TGGCCA 1 cut(s) 241
MluCI AATT 1 cut(s) 69
MluNI TGGCCA 1 cut(s) 241
MlyI GAGTC 1 cut(s) 398
MmeI TCCRAC 2 cut(s) 372, 673
MnlI CCTC 8 cut(s) 36, 178, 207, 376, 394, 528, 705, 708
Mox20I TGGCCA 1 cut(s) 241
Mph1103I ATGCAT 1 cut(s) 774
MscI TGGCCA 1 cut(s) 241
MseI TTAA 1 cut(s) 59
MslI CAYNNNNRTG 2 cut(s) 555, 558
Msp20I TGGCCA 1 cut(s) 241
MspA1I CMGCKG 2 cut(s) 17, 154
MspI CCGG 5 cut(s) 543, 569, 662, 701, 794
MspR9I CCNGG 4 cut(s) 30, 543, 662, 663
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 5 cut(s) 205, 232, 414, 534, 569
NciI CCSGG 3 cut(s) 543, 662, 663
NdeI CATATG 1 cut(s) 654
NdeII GATC 3 cut(s) 32, 645, 814
NlaIII CATG 4 cut(s) 54, 610, 631, 776
NlaIV GGNNCC 3 cut(s) 34, 469, 666
NmuCI GTSAC 2 cut(s) 163, 169
NsiI ATGCAT 1 cut(s) 774
NspI RCATGY 1 cut(s) 610
PciI ACATGT 1 cut(s) 606
PfeI GAWTC 4 cut(s) 146, 514, 631, 824
PfoI TCCNGGA 1 cut(s) 28
PkrI GCNGC 9 cut(s) 86, 156, 451, 530, 588, 625, 706, 857, 860
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
Ppu21I YACGTR 1 cut(s) 783
PscI ACATGT 1 cut(s) 606
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 3 cut(s) 34, 469, 666
PspPI GGNCC 4 cut(s) 584, 599, 638, 665
PstNI CAGNNNCTG 1 cut(s) 173
PsuI RGATCY 1 cut(s) 32
PvuII CAGCTG 2 cut(s) 17, 154
RsaI GTAC 3 cut(s) 659, 727, 785
RsaNI GTAC 3 cut(s) 658, 726, 784
RseI CAYNNNNRTG 2 cut(s) 555, 558
SaqAI TTAA 1 cut(s) 59
SatI GCNGC 9 cut(s) 85, 155, 450, 529, 587, 624, 705, 856, 859
Sau3AI GATC 3 cut(s) 32, 645, 814
Sau96I GGNCC 4 cut(s) 584, 599, 638, 665
SchI GAGTC 1 cut(s) 398
ScrFI CCNGG 4 cut(s) 30, 543, 662, 663
SfaNI GCATC 3 cut(s) 358, 550, 559
SinI GGWCC 2 cut(s) 599, 665
SmaI CCCGGG 1 cut(s) 663
SmiMI CAYNNNNRTG 2 cut(s) 555, 558
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
Sse9I AATT 1 cut(s) 69
SsiI CCGC 5 cut(s) 528, 587, 674, 705, 855
StyD4I CCNGG 4 cut(s) 28, 541, 660, 661
TaaI ACNGT 3 cut(s) 169, 557, 725
TaiI ACGT 4 cut(s) 183, 721, 734, 785
TaqI TCGA 1 cut(s) 822
TasI AATT 1 cut(s) 69
TauI GCSGC 4 cut(s) 531, 589, 707, 858
TfiI GAWTC 4 cut(s) 146, 514, 631, 824
Tru1I TTAA 1 cut(s) 59
Tru9I TTAA 1 cut(s) 59
TscAI CASTG 1 cut(s) 174
TseFI GTSAC 2 cut(s) 163, 169
TseI GCWGC 5 cut(s) 84, 154, 449, 623, 858
Tsp45I GTSAC 2 cut(s) 163, 169
TspDTI ATGAA 7 cut(s) 62, 327, 366, 435, 644, 741, 861
TspMI CCCGGG 1 cut(s) 661
TspRI CASTG 1 cut(s) 174
VpaK11BI GGWCC 2 cut(s) 599, 665
XceI RCATGY 1 cut(s) 610
XmaI CCCGGG 1 cut(s) 661
Zsp2I ATGCAT 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.