MD11G1304000.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011694
Physical Location & Seq
Forward (+)
41905287 .. 41907154
1868 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1304000.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGACCACATCAGATGCTTTCTTCACACTCTTTAAACCTCAGAACACATTCTCCCAAAGCTCCCCACTTCCCTTATTCTCCACTTCATCACCCTCTCTCTCCTCTCTCTCTCTTTCTCTCTCTCTCTCATCAATGGCAACAAAACCGGCTGAAGAAGCTCTTGGAACCTTAAAATACCAGACATGGGTCTTGAAGGTCTCAATCCACTGTGAAGGATGCAAGAGGAAAGTCAAGAAAGTTCTTCAGAACATCGATGGGGTTTACACGACGGATATTGATTCGCAGCAGCACAAAGTGACTGTGACCGGCAACGTGGAGGCGGAGACTCTACTGAAGAAGCTGCAGAAGTCAGGGAAGCACGCCGAGCTTTGGCCGGAAGTTAAGAAATCGAAAAAGTCCGGGAAATCAAAGAATAGTAGTAAGAACAGTGAGAAGCAGCCCGCGGATCATAACCATGAACAAGATGGTAAACCGGATAAAACCAACGGCGGTGATGATGCTGACGGCAGCGGTGAATCCGACGCAGAGGGTGACGAAGGTAATGAAGGTGGTGGAGAAAGCGGTGCTAGTCCTGCTGTTGGCGGTGGCGGCGGAAACACAGCAAGTACCGGGGCCAAAAAGAAGAAGAAAAAGAAGAAGAAGAAAGGGCAAAATAGTAATTCCACAAATGGCGGCGGTGCCACTGCCAACCCCGGCGAGCACAATATTGGAGATGCTCCGCCTCCTGCGGGCAGTATTGGGCCGTATATGGCTGGTGGGCCAGACATGACCCAACCTATAGCCTCCATGAATCTTGGCCCACCAGTTCAGCATGCGTACCCCGGCCCGTACCCAACATCAATGTACTATCCGCCCCCAACGTACGGGCTAAGTTACAATACGGCGTACCCTACCAGTACAAGTACCTCATACTATGCTCCTGATCCCATGCATACTGCCACTTATTCTCATCCGGGCATGTTCTCGTTTGCTCTGCCGTCCGATTCTATCAATGACGATGATGAAGACACGTTCGTTGACGATGAAGATGAAATTGGGTGCTACATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

351

Amino Acids

36.95

Weight (kDa)

5.74

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 64 - 120 5e-15 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011694)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27690
fragaria_vesca FvH4_2g25240 FvH4_3g05420
malus_domestica MD03G1282300.v1.1 MD11G1304000.v1.1
prunus_persica Prupe.8G257200_v2.0.a1
pyrus_communis pycom03g22310 pycom11g26560
rosa_chinensis RchiOBHm_Chr6g0293381
rosa_laevigata RLG00000011951
rosa_multiflora Rmu_sc0006269.1_g000028
rosa_roxburghii Rroxscaffold_7G00174210
rosa_rugosa Rorug06G0235000 Rorug06G0235100
rosa_samantha Rh6AG347900 Rh6BG355500 Rh6CG361600 Rh6DG348700
rosa_wichuraiana Rw6G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 677
AccII CGCG 1 cut(s) 443
AclWI GGATC 2 cut(s) 453, 917
AcoI YGGCCR 1 cut(s) 371
AcuI CTGAAG 3 cut(s) 171, 227, 353
AdeI CACNNNGTG 1 cut(s) 295
AfaI GTAC 8 cut(s) 607, 818, 830, 845, 863, 887, 898, 904
AfiI CCNNNNNNNGG 5 cut(s) 184, 369, 578, 728, 863
AflIII ACRYGT 1 cut(s) 1008
AgsI TTSAA 1 cut(s) 193
AjuI GAANNNNNNNTTGG 4 cut(s) 144, 176, 1017, 1049
AloI GAACNNNNNNTCC 2 cut(s) 35, 67
AluBI AGCT 4 cut(s) 60, 158, 340, 367
AluI AGCT 4 cut(s) 60, 158, 340, 367
Alw21I GWGCWC 1 cut(s) 702
Alw26I GTCTC 2 cut(s) 202, 317
AlwI GGATC 2 cut(s) 453, 917
AoxI GGCC 6 cut(s) 371, 612, 740, 758, 796, 823
ApeKI GCWGC 5 cut(s) 283, 286, 340, 436, 507
Asp700I GAANNNNTTC 1 cut(s) 47
AspS9I GGNCC 5 cut(s) 612, 740, 758, 797, 824
AsuC2I CCSGG 5 cut(s) 400, 610, 693, 822, 954
AsuHPI GGTGA 4 cut(s) 81, 503, 524, 542
BanI GGYRCC 1 cut(s) 677
BbsI GAAGAC 1 cut(s) 1011
Bbv12I GWGCWC 1 cut(s) 702
BbvI GCAGC 5 cut(s) 295, 298, 327, 448, 519
BccI CCATC 2 cut(s) 248, 458
BceAI ACGGC 5 cut(s) 502, 520, 727, 897, 961
BcnI CCSGG 5 cut(s) 400, 610, 693, 822, 954
BcoDI GTCTC 2 cut(s) 202, 317
BfaI CTAG 1 cut(s) 567
BfmI CTRYAG 2 cut(s) 341, 777
BisI GCNGC 7 cut(s) 284, 287, 341, 437, 508, 589, 673
BlsI GCNGC 7 cut(s) 285, 288, 342, 438, 509, 590, 674
Bme1390I CCNGG 5 cut(s) 400, 610, 693, 822, 954
BmgT120I GGNCC 5 cut(s) 612, 740, 758, 797, 824
BmiI GGNNCC 3 cut(s) 166, 613, 679
BmrFI CCNGG 5 cut(s) 400, 610, 693, 822, 954
BmsI GCATC 4 cut(s) 4, 206, 487, 703
BoxI GACNNNNGTC 1 cut(s) 185
BpiI GAAGAC 1 cut(s) 1011
BpuMI CCSGG 5 cut(s) 400, 610, 693, 822, 954
Bsa29I ATCGAT 1 cut(s) 252
BsaI GGTCTC 1 cut(s) 202
BsaJI CCNNGG 4 cut(s) 441, 609, 691, 820
BsaWI WCCGGW 1 cut(s) 472
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 5 cut(s) 184, 369, 578, 728, 863
Bse118I RCCGGY 2 cut(s) 145, 305
Bse1I ACTGG 2 cut(s) 803, 894
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 4 cut(s) 441, 609, 691, 820
BseGI GGATG 2 cut(s) 221, 949
BseLI CCNNNNNNNGG 5 cut(s) 184, 369, 578, 728, 863
BseMII CTCAG 1 cut(s) 53
BseNI ACTGG 2 cut(s) 803, 894
BseRI GAGGAG 1 cut(s) 91
BseXI GCAGC 5 cut(s) 295, 298, 327, 448, 519
Bsh1236I CGCG 1 cut(s) 443
BshFI GGCC 6 cut(s) 373, 614, 742, 760, 798, 825
BshNI GGYRCC 1 cut(s) 677
BshVI ATCGAT 1 cut(s) 252
BsiHKAI GWGCWC 1 cut(s) 702
BsiSI CCGG 9 cut(s) 146, 306, 374, 399, 473, 609, 693, 822, 953
BsiWI CGTACG 1 cut(s) 861
BslI CCNNNNNNNGG 5 cut(s) 184, 369, 578, 728, 863
BsmAI GTCTC 2 cut(s) 202, 317
BsnI GGCC 6 cut(s) 373, 614, 742, 760, 798, 825
Bso31I GGTCTC 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 702
Bsp143I GATC 2 cut(s) 445, 922
BspANI GGCC 6 cut(s) 373, 614, 742, 760, 798, 825
BspCNI CTCAG 1 cut(s) 52
BspDI ATCGAT 1 cut(s) 252
BspFNI CGCG 1 cut(s) 443
BspLI GGNNCC 3 cut(s) 166, 613, 679
BspMAI CTGCAG 1 cut(s) 345
BspPI GGATC 2 cut(s) 453, 917
BspT107I GGYRCC 1 cut(s) 677
BspTNI GGTCTC 1 cut(s) 202
BsrFI RCCGGY 2 cut(s) 145, 305
BsrI ACTGG 2 cut(s) 803, 894
BssAI RCCGGY 2 cut(s) 145, 305
BssECI CCNNGG 4 cut(s) 441, 609, 691, 820
BssMI GATC 2 cut(s) 445, 922
Bst4CI ACNGT 3 cut(s) 209, 301, 428
BstC8I GCNNGC 5 cut(s) 360, 441, 698, 730, 813
BstDEI CTNAG 2 cut(s) 39, 869
BstDSI CCRYGG 1 cut(s) 441
BstF5I GGATG 2 cut(s) 221, 949
BstFNI CGCG 1 cut(s) 443
BstKTI GATC 2 cut(s) 448, 925
BstMAI GTCTC 2 cut(s) 202, 317
BstMBI GATC 2 cut(s) 445, 922
BstMWI GCNNNNNNNGC 4 cut(s) 155, 364, 572, 588
BstNSI RCATGY 2 cut(s) 815, 961
BstPAI GACNNNNGTC 1 cut(s) 185
BstSCI CCNGG 5 cut(s) 398, 608, 691, 820, 952
BstSFI CTRYAG 2 cut(s) 341, 777
BstUI CGCG 1 cut(s) 443
BstV1I GCAGC 5 cut(s) 295, 298, 327, 448, 519
BstV2I GAAGAC 1 cut(s) 1011
Bsu15I ATCGAT 1 cut(s) 252
BsuRI GGCC 6 cut(s) 373, 614, 742, 760, 798, 825
BsuTUI ATCGAT 1 cut(s) 252
BtgI CCRYGG 1 cut(s) 441
BtsCI GGATG 2 cut(s) 221, 949
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 3 cut(s) 205, 433, 681
Cac8I GCNNGC 5 cut(s) 360, 441, 698, 730, 813
Cfr10I RCCGGY 2 cut(s) 145, 305
Cfr13I GGNCC 5 cut(s) 612, 740, 758, 797, 824
Cfr42I CCGCGG 1 cut(s) 444
ClaI ATCGAT 1 cut(s) 252
CseI GACGC 1 cut(s) 530
Csp6I GTAC 8 cut(s) 606, 817, 829, 844, 862, 886, 897, 903
CviAII CATG 7 cut(s) 183, 455, 766, 787, 812, 928, 958
CviQI GTAC 8 cut(s) 606, 817, 829, 844, 862, 886, 897, 903
DdeI CTNAG 2 cut(s) 39, 869
DpnI GATC 2 cut(s) 447, 924
DpnII GATC 2 cut(s) 445, 922
DraI TTTAAA 1 cut(s) 34
DraIII CACNNNGTG 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 371
EciI GGCGGA 4 cut(s) 335, 606, 708, 840
Eco31I GGTCTC 1 cut(s) 202
Eco57I CTGAAG 3 cut(s) 171, 227, 353
EcoT22I ATGCAT 1 cut(s) 933
FaeI CATG 7 cut(s) 186, 458, 769, 790, 815, 931, 961
FalI AAGNNNNNCTT 2 cut(s) 144, 176
FatI CATG 7 cut(s) 182, 454, 765, 786, 811, 927, 957
FauI CCCGC 2 cut(s) 448, 721
Fnu4HI GCNGC 7 cut(s) 284, 287, 341, 437, 508, 589, 673
FokI GGATG 2 cut(s) 228, 936
Fsp4HI GCNGC 7 cut(s) 284, 287, 341, 437, 508, 589, 673
FspBI CTAG 1 cut(s) 567
GluI GCNGC 7 cut(s) 284, 287, 341, 437, 508, 589, 673
HaeIII GGCC 6 cut(s) 373, 614, 742, 760, 798, 825
HapII CCGG 9 cut(s) 146, 306, 374, 399, 473, 609, 693, 822, 953
HgaI GACGC 1 cut(s) 530
Hin1II CATG 7 cut(s) 186, 458, 769, 790, 815, 931, 961
HincII GTYRAC 1 cut(s) 1018
HindII GTYRAC 1 cut(s) 1018
HinfI GANTC 5 cut(s) 278, 325, 515, 790, 983
HpaII CCGG 9 cut(s) 146, 306, 374, 399, 473, 609, 693, 822, 953
HphI GGTGA 4 cut(s) 81, 503, 524, 542
Hpy166II GTNNAC 3 cut(s) 262, 470, 1018
Hpy188I TCNGA 5 cut(s) 13, 42, 246, 520, 982
Hpy188III TCNNGA 3 cut(s) 190, 232, 920
Hpy8I GTNNAC 3 cut(s) 262, 470, 1018
Hpy99I CGWCG 2 cut(s) 271, 524
HpyAV CCTTC 4 cut(s) 187, 206, 530, 539
HpyCH4III ACNGT 3 cut(s) 209, 301, 428
HpyCH4IV ACGT 3 cut(s) 312, 860, 1010
HpyCH4V TGCA 3 cut(s) 219, 343, 931
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 364, 572, 588
HpyF3I CTNAG 2 cut(s) 39, 869
HpySE526I ACGT 3 cut(s) 312, 860, 1010
Hsp92II CATG 7 cut(s) 186, 458, 769, 790, 815, 931, 961
KspI CCGCGG 1 cut(s) 444
Kzo9I GATC 2 cut(s) 445, 922
LmnI GCTCC 3 cut(s) 65, 721, 922
Lsp1109I GCAGC 5 cut(s) 295, 298, 327, 448, 519
LweI GCATC 4 cut(s) 4, 206, 487, 703
MaeI CTAG 1 cut(s) 567
MaeII ACGT 3 cut(s) 312, 860, 1010
MaeIII GTNAC 4 cut(s) 295, 301, 530, 872
MalI GATC 2 cut(s) 447, 924
MboI GATC 2 cut(s) 445, 922
MhlI GDGCHC 1 cut(s) 702
MluCI AATT 2 cut(s) 658, 1032
MlyI GAGTC 1 cut(s) 319
MmeI TCCRAC 1 cut(s) 543
MnlI CCTC 9 cut(s) 48, 103, 112, 216, 310, 520, 732, 793, 916
Mph1103I ATGCAT 1 cut(s) 933
MroXI GAANNNNTTC 1 cut(s) 47
MseI TTAA 3 cut(s) 33, 170, 381
MslI CAYNNNNRTG 1 cut(s) 453
MspA1I CMGCKG 2 cut(s) 443, 510
MspI CCGG 9 cut(s) 146, 306, 374, 399, 473, 609, 693, 822, 953
MspR9I CCNGG 5 cut(s) 400, 610, 693, 822, 954
MvnI CGCG 1 cut(s) 443
MwoI GCNNNNNNNGC 4 cut(s) 155, 364, 572, 588
NciI CCSGG 5 cut(s) 400, 610, 693, 822, 954
NdeII GATC 2 cut(s) 445, 922
NlaIII CATG 7 cut(s) 186, 458, 769, 790, 815, 931, 961
NlaIV GGNNCC 3 cut(s) 166, 613, 679
NmeAIII GCCGAG 1 cut(s) 388
NmuCI GTSAC 3 cut(s) 295, 301, 530
NsiI ATGCAT 1 cut(s) 933
NspI RCATGY 2 cut(s) 815, 961
PaeI GCATGC 1 cut(s) 815
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 4 cut(s) 278, 515, 790, 983
Pfl23II CGTACG 1 cut(s) 861
PfoI TCCNGGA 1 cut(s) 398
PkrI GCNGC 7 cut(s) 285, 288, 342, 438, 509, 590, 674
PleI GAGTC 1 cut(s) 319
PpsI GAGTC 1 cut(s) 319
PshAI GACNNNNGTC 1 cut(s) 185
PspLI CGTACG 1 cut(s) 861
PspN4I GGNNCC 3 cut(s) 166, 613, 679
PspPI GGNCC 5 cut(s) 612, 740, 758, 797, 824
PstI CTGCAG 1 cut(s) 345
RsaI GTAC 8 cut(s) 607, 818, 830, 845, 863, 887, 898, 904
RsaNI GTAC 8 cut(s) 606, 817, 829, 844, 862, 886, 897, 903
RseI CAYNNNNRTG 1 cut(s) 453
SacII CCGCGG 1 cut(s) 444
SaqAI TTAA 3 cut(s) 33, 170, 381
SatI GCNGC 7 cut(s) 284, 287, 341, 437, 508, 589, 673
Sau3AI GATC 2 cut(s) 445, 922
Sau96I GGNCC 5 cut(s) 612, 740, 758, 797, 824
SchI GAGTC 1 cut(s) 319
ScrFI CCNGG 5 cut(s) 400, 610, 693, 822, 954
SduI GDGCHC 1 cut(s) 702
SfaNI GCATC 4 cut(s) 4, 206, 487, 703
SfcI CTRYAG 2 cut(s) 341, 777
Sfr303I CCGCGG 1 cut(s) 444
SgrBI CCGCGG 1 cut(s) 444
SmiMI CAYNNNNRTG 1 cut(s) 453
SphI GCATGC 1 cut(s) 815
Sse9I AATT 2 cut(s) 658, 1032
SspI AATATT 1 cut(s) 706
SspMI CTAG 1 cut(s) 567
StyD4I CCNGG 5 cut(s) 398, 608, 691, 820, 952
TaaI ACNGT 3 cut(s) 209, 301, 428
TaiI ACGT 3 cut(s) 315, 863, 1013
TaqI TCGA 2 cut(s) 252, 389
TasI AATT 2 cut(s) 658, 1032
TatI WGTACW 2 cut(s) 843, 896
TauI GCSGC 2 cut(s) 591, 675
TfiI GAWTC 4 cut(s) 278, 515, 790, 983
Tru1I TTAA 3 cut(s) 33, 170, 381
Tru9I TTAA 3 cut(s) 33, 170, 381
TscAI CASTG 3 cut(s) 212, 433, 688
TseFI GTSAC 3 cut(s) 295, 301, 530
TseI GCWGC 5 cut(s) 283, 286, 340, 436, 507
Tsp45I GTSAC 3 cut(s) 295, 301, 530
TspDTI ATGAA 7 cut(s) 75, 471, 558, 803, 1017, 1038, 1044
TspGWI ACGGA 1 cut(s) 284
TspRI CASTG 3 cut(s) 212, 433, 688
XceI RCATGY 2 cut(s) 815, 961
XcmI CCANNNNNNNNNTGG 1 cut(s) 461
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 1 cut(s) 567
Zsp2I ATGCAT 1 cut(s) 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.