Prupe.8G257200_v2.0.a1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0011694
Physical Location & Seq
Forward (+)
21908574 .. 21910474
1901 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G257200.1

Sequence Viewer

Length: 891 bp
ATGGCAACAAAACCAGCTGAAGAAGCTCCTGGATCCTTAAAATACCAGACATGGGTCCTGAAAGTCTCAATTCACTGTGAAGGCTGCAAGAAGAAAGTGAAGAAAGTCCTTAAAGGCATTGAAGGGGTTTACACGACGACGGTTGATTCTCAGCAGCACAAGGTGACGGTGACTGGCAACGTGGAGGCAGAGACCCTTCTCAAGAAGCTGTTGAGGTCAGGCAAACACGCCGAGCTTTGGCCTGAAACTAAGAAAGCCAAAAAATCCAAATCAAAGAATAATAATAATGAAAAGAAGGCCACAGATGGTAGTGAAAAAGATGGCGATCATGACGATGGCCATGGTGATAAACCTGATCAAATCAACGGCGATGATGATGCTGATGATGGTGGTGAGTCGGACAAAGAGGGGGACGAAAGTAATGAAGGTGCTGGTGGTGGTGGAAATAATGCAAGTACTGGGGCTAAAAAGAAGAAAAAGAAGAAGAAGAAGAAAGGGCAAAATGATAATTCTACTAATGTCAGCGGCGGTGGCAAACCCAGTGACACATATTTTGGTGATGCTCCGGGTATCGGAATTGTGCCGTCTATGGCTGCTCATGAGGTGACTCAAGCGATGGGCTCCATGAATATGGGCCCACCAATTCAGCACGTATACCCCGGCCCATACCCTTCATCAATGTACTATCAGCCTCCAACATATGGGCTAAGTTACAATACGGCCTATCCTACGAGTACAGCTACTTCATACTTTGCTCCTGATGCCATGCATTCTACTACGTATTCTCACCCGGATATGTACTCGCCTGCTCTGCCGTTCGATTCCTTCGAAACATTCTATGTGGAGGATGACGACCACGATGGAGACGAAAGTGGGTGTCACATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

297

Amino Acids

31.85

Weight (kDa)

5.78

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011694)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27690
fragaria_vesca FvH4_2g25240 FvH4_3g05420
malus_domestica MD03G1282300.v1.1 MD11G1304000.v1.1
prunus_persica Prupe.8G257200_v2.0.a1
pyrus_communis pycom03g22310 pycom11g26560
rosa_chinensis RchiOBHm_Chr6g0293381
rosa_laevigata RLG00000011951
rosa_multiflora Rmu_sc0006269.1_g000028
rosa_roxburghii Rroxscaffold_7G00174210
rosa_rugosa Rorug06G0235000 Rorug06G0235100
rosa_samantha Rh6AG347900 Rh6BG355500 Rh6CG361600 Rh6DG348700
rosa_wichuraiana Rw6G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 701
AccI GTMKAC 1 cut(s) 654
AciI CCGC 2 cut(s) 525, 528
AclWI GGATC 2 cut(s) 27, 40
AcoI YGGCCR 1 cut(s) 337
AcuI CTGAAG 1 cut(s) 39
AdeI CACNNNGTG 1 cut(s) 163
AfaI GTAC 4 cut(s) 457, 683, 736, 800
AfiI CCNNNNNNNGG 4 cut(s) 52, 237, 572, 701
AgsI TTSAA 1 cut(s) 122
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 5 cut(s) 17, 26, 208, 235, 740
AluI AGCT 5 cut(s) 17, 26, 208, 235, 740
Alw26I GTCTC 3 cut(s) 70, 185, 858
AlwI GGATC 2 cut(s) 27, 40
AoxI GGCC 6 cut(s) 239, 297, 337, 634, 661, 720
ApaI GGGCCC 1 cut(s) 638
ApeKI GCWGC 3 cut(s) 84, 154, 593
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AspS9I GGNCC 4 cut(s) 55, 634, 635, 662
AsuC2I CCSGG 3 cut(s) 567, 660, 791
AsuHPI GGTGA 7 cut(s) 175, 181, 356, 404, 569, 616, 779
AsuII TTCGAA 1 cut(s) 828
AvaII GGWCC 1 cut(s) 55
BaeGI GKGCMC 1 cut(s) 638
BalI TGGCCA 1 cut(s) 339
BamHI GGATCC 1 cut(s) 32
BanII GRGCYC 2 cut(s) 623, 638
BarI GAAGNNNNNNTAC 2 cut(s) 727, 759
BbvI GCAGC 3 cut(s) 71, 166, 580
BccI CCATC 6 cut(s) 299, 314, 329, 380, 610, 854
BceAI ACGGC 4 cut(s) 382, 568, 735, 799
BcgI CGANNNNNNTGC 2 cut(s) 359, 393
BciT130I CCWGG 1 cut(s) 30
BclI TGATCA 1 cut(s) 355
BcnI CCSGG 3 cut(s) 567, 660, 791
BcoDI GTCTC 3 cut(s) 70, 185, 858
BisI GCNGC 4 cut(s) 85, 155, 526, 594
BlsI GCNGC 4 cut(s) 86, 156, 527, 595
BmcAI AGTACT 1 cut(s) 457
Bme1390I CCNGG 4 cut(s) 30, 567, 660, 791
Bme18I GGWCC 1 cut(s) 55
BmgT120I GGNCC 4 cut(s) 55, 634, 635, 662
BmiI GGNNCC 4 cut(s) 34, 56, 622, 636
BmrFI CCNGG 4 cut(s) 30, 567, 660, 791
BmrI ACTGGG 2 cut(s) 468, 534
BmsI GCATC 3 cut(s) 367, 550, 751
BmuI ACTGGG 2 cut(s) 468, 534
BoxI GACNNNNGTC 1 cut(s) 53
Bpu14I TTCGAA 1 cut(s) 828
BpuEI CTTGAG 2 cut(s) 185, 594
BpuMI CCSGG 3 cut(s) 567, 660, 791
BsaAI YACGTR 2 cut(s) 652, 780
BsaBI GATNNNNATC 1 cut(s) 324
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 2 cut(s) 340, 658
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 237, 572, 701
Bse1I ACTGG 3 cut(s) 178, 463, 540
Bse8I GATNNNNATC 1 cut(s) 324
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 2 cut(s) 340, 658
BseGI GGATG 1 cut(s) 853
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 4 cut(s) 52, 237, 572, 701
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 3 cut(s) 178, 463, 540
BseSI GKGCMC 1 cut(s) 638
BseXI GCAGC 3 cut(s) 71, 166, 580
BshFI GGCC 6 cut(s) 241, 299, 339, 636, 663, 722
BsiSI CCGG 3 cut(s) 566, 660, 791
BslFI GGGAC 1 cut(s) 425
BslI CCNNNNNNNGG 4 cut(s) 52, 237, 572, 701
BsmAI GTCTC 3 cut(s) 70, 185, 858
BsmBI CGTCTC 1 cut(s) 858
BsmFI GGGAC 1 cut(s) 425
BsmI GAATGC 1 cut(s) 769
BsnI GGCC 6 cut(s) 241, 299, 339, 636, 663, 722
Bso31I GGTCTC 1 cut(s) 185
Bsp119I TTCGAA 1 cut(s) 828
Bsp120I GGGCCC 1 cut(s) 634
Bsp1286I GDGCHC 2 cut(s) 623, 638
Bsp143I GATC 3 cut(s) 32, 325, 355
Bsp19I CCATGG 1 cut(s) 340
BspACI CCGC 2 cut(s) 525, 528
BspANI GGCC 6 cut(s) 241, 299, 339, 636, 663, 722
BspCNI CTCAG 1 cut(s) 163
BspHI TCATGA 2 cut(s) 328, 598
BspLI GGNNCC 4 cut(s) 34, 56, 622, 636
BspPI GGATC 2 cut(s) 27, 40
BspT104I TTCGAA 1 cut(s) 828
BspTNI GGTCTC 1 cut(s) 185
BsrI ACTGG 3 cut(s) 178, 463, 540
BssECI CCNNGG 2 cut(s) 340, 658
BssMI GATC 3 cut(s) 32, 325, 355
BssNAI GTATAC 1 cut(s) 655
BssT1I CCWWGG 1 cut(s) 340
Bst1107I GTATAC 1 cut(s) 655
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 3 cut(s) 77, 142, 169
BstBAI YACGTR 2 cut(s) 652, 780
BstBI TTCGAA 1 cut(s) 828
BstC8I GCNNGC 1 cut(s) 807
BstDEI CTNAG 3 cut(s) 150, 249, 707
BstDSI CCRYGG 1 cut(s) 340
BstF5I GGATG 1 cut(s) 853
BstKTI GATC 3 cut(s) 35, 328, 358
BstMAI GTCTC 3 cut(s) 70, 185, 858
BstMBI GATC 3 cut(s) 32, 325, 355
BstMWI GCNNNNNNNGC 4 cut(s) 23, 531, 761, 811
BstNI CCWGG 1 cut(s) 30
BstPAI GACNNNNGTC 1 cut(s) 53
BstSCI CCNGG 4 cut(s) 28, 565, 658, 789
BstSLI GKGCMC 1 cut(s) 638
BstSNI TACGTA 1 cut(s) 780
BstV1I GCAGC 3 cut(s) 71, 166, 580
BstX2I RGATCY 1 cut(s) 32
BstXI CCANNNNNNTGG 1 cut(s) 631
BstYI RGATCY 1 cut(s) 32
BstZ17I GTATAC 1 cut(s) 655
BsuRI GGCC 6 cut(s) 241, 299, 339, 636, 663, 722
BtgI CCRYGG 1 cut(s) 340
BtgZI GCGATG 2 cut(s) 384, 629
BtsCI GGATG 1 cut(s) 853
BtsIMutI CAGTG 2 cut(s) 73, 547
Cac8I GCNNGC 1 cut(s) 807
CciI TCATGA 2 cut(s) 328, 598
Cfr13I GGNCC 4 cut(s) 55, 634, 635, 662
Csp6I GTAC 4 cut(s) 456, 682, 735, 799
CviAII CATG 6 cut(s) 51, 329, 341, 599, 625, 766
CviQI GTAC 4 cut(s) 456, 682, 735, 799
DdeI CTNAG 3 cut(s) 150, 249, 707
DpnI GATC 3 cut(s) 34, 327, 357
DpnII GATC 3 cut(s) 32, 325, 355
DraIII CACNNNGTG 1 cut(s) 163
EaeI YGGCCR 1 cut(s) 337
Eco105I TACGTA 1 cut(s) 780
Eco130I CCWWGG 1 cut(s) 340
Eco24I GRGCYC 2 cut(s) 623, 638
Eco31I GGTCTC 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 39
EcoO109I RGGNCCY 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 340
EcoT22I ATGCAT 1 cut(s) 771
EcoT38I GRGCYC 2 cut(s) 623, 638
ErhI CCWWGG 1 cut(s) 340
Esp3I CGTCTC 1 cut(s) 858
FaeI CATG 6 cut(s) 54, 332, 344, 602, 628, 769
FaqI GGGAC 1 cut(s) 425
FatI CATG 6 cut(s) 50, 328, 340, 598, 624, 765
FauNDI CATATG 1 cut(s) 700
FbaI TGATCA 1 cut(s) 355
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 4 cut(s) 85, 155, 526, 594
FokI GGATG 1 cut(s) 860
FriOI GRGCYC 2 cut(s) 623, 638
Fsp4HI GCNGC 4 cut(s) 85, 155, 526, 594
GluI GCNGC 4 cut(s) 85, 155, 526, 594
HaeIII GGCC 6 cut(s) 241, 299, 339, 636, 663, 722
HapII CCGG 3 cut(s) 566, 660, 791
Hin1II CATG 6 cut(s) 54, 332, 344, 602, 628, 769
HinfI GANTC 4 cut(s) 146, 395, 607, 821
HpaII CCGG 3 cut(s) 566, 660, 791
HphI GGTGA 7 cut(s) 175, 181, 356, 404, 569, 616, 779
Hpy166II GTNNAC 2 cut(s) 130, 655
Hpy188I TCNGA 2 cut(s) 400, 575
Hpy188III TCNNGA 5 cut(s) 58, 202, 329, 599, 758
Hpy8I GTNNAC 2 cut(s) 130, 655
Hpy99I CGWCG 2 cut(s) 139, 142
HpyAV CCTTC 7 cut(s) 74, 116, 206, 289, 419, 681, 835
HpyCH4III ACNGT 3 cut(s) 77, 142, 169
HpyCH4IV ACGT 3 cut(s) 180, 651, 779
HpyCH4V TGCA 3 cut(s) 87, 452, 769
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 531, 761, 811
HpyF3I CTNAG 3 cut(s) 150, 249, 707
HpySE526I ACGT 3 cut(s) 180, 651, 779
Hsp92II CATG 6 cut(s) 54, 332, 344, 602, 628, 769
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 3 cut(s) 32, 325, 355
LmnI GCTCC 4 cut(s) 31, 568, 626, 760
Lsp1109I GCAGC 3 cut(s) 71, 166, 580
LweI GCATC 3 cut(s) 367, 550, 751
MaeII ACGT 3 cut(s) 180, 651, 779
MaeIII GTNAC 6 cut(s) 163, 169, 542, 604, 710, 878
MalI GATC 3 cut(s) 34, 327, 357
MboI GATC 3 cut(s) 32, 325, 355
MboII GAAGA 8 cut(s) 32, 103, 112, 484, 493, 496, 499, 502
MflI RGATCY 1 cut(s) 32
MhlI GDGCHC 2 cut(s) 623, 638
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 4 cut(s) 69, 508, 576, 642
MluNI TGGCCA 1 cut(s) 339
MlyI GAGTC 2 cut(s) 404, 601
MmeI TCCRAC 2 cut(s) 378, 719
MnlI CCTC 6 cut(s) 178, 207, 400, 595, 702, 838
Mox20I TGGCCA 1 cut(s) 339
Mph1103I ATGCAT 1 cut(s) 771
MscI TGGCCA 1 cut(s) 339
MseI TTAA 2 cut(s) 38, 111
MslI CAYNNNNRTG 2 cut(s) 333, 629
Msp20I TGGCCA 1 cut(s) 339
MspA1I CMGCKG 2 cut(s) 17, 525
MspI CCGG 3 cut(s) 566, 660, 791
MspR9I CCNGG 4 cut(s) 30, 567, 660, 791
Mva1269I GAATGC 1 cut(s) 769
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 4 cut(s) 23, 531, 761, 811
NciI CCSGG 3 cut(s) 567, 660, 791
NcoI CCATGG 1 cut(s) 340
NdeI CATATG 1 cut(s) 700
NdeII GATC 3 cut(s) 32, 325, 355
NlaIII CATG 6 cut(s) 54, 332, 344, 602, 628, 769
NlaIV GGNNCC 4 cut(s) 34, 56, 622, 636
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 5 cut(s) 163, 169, 542, 604, 878
NsiI ATGCAT 1 cut(s) 771
NspV TTCGAA 1 cut(s) 828
PagI TCATGA 2 cut(s) 328, 598
PcsI WCGNNNNNNNCGW 2 cut(s) 825, 864
PctI GAATGC 1 cut(s) 769
PfeI GAWTC 2 cut(s) 146, 821
PflMI CCANNNNNTGG 1 cut(s) 701
PfoI TCCNGGA 1 cut(s) 28
PkrI GCNGC 4 cut(s) 86, 156, 527, 595
PleI GAGTC 2 cut(s) 403, 601
PpsI GAGTC 2 cut(s) 403, 601
Ppu21I YACGTR 2 cut(s) 652, 780
PpuMI RGGWCCY 1 cut(s) 55
PshAI GACNNNNGTC 1 cut(s) 53
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 4 cut(s) 34, 56, 622, 636
PspOMI GGGCCC 1 cut(s) 634
PspPI GGNCC 4 cut(s) 55, 634, 635, 662
PspPPI RGGWCCY 1 cut(s) 55
PsuI RGATCY 1 cut(s) 32
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 4 cut(s) 457, 683, 736, 800
RsaNI GTAC 4 cut(s) 456, 682, 735, 799
RseI CAYNNNNRTG 2 cut(s) 333, 629
SaqAI TTAA 2 cut(s) 38, 111
SatI GCNGC 4 cut(s) 85, 155, 526, 594
Sau3AI GATC 3 cut(s) 32, 325, 355
Sau96I GGNCC 4 cut(s) 55, 634, 635, 662
ScaI AGTACT 1 cut(s) 457
SchI GAGTC 2 cut(s) 404, 601
ScrFI CCNGG 4 cut(s) 30, 567, 660, 791
SduI GDGCHC 2 cut(s) 623, 638
SfaNI GCATC 3 cut(s) 367, 550, 751
SfuI TTCGAA 1 cut(s) 828
SinI GGWCC 1 cut(s) 55
SmiMI CAYNNNNRTG 2 cut(s) 333, 629
SmlI CTYRAG 2 cut(s) 200, 609
SmoI CTYRAG 2 cut(s) 200, 609
SnaBI TACGTA 1 cut(s) 780
Sse9I AATT 4 cut(s) 69, 508, 576, 642
SsiI CCGC 2 cut(s) 525, 528
StyD4I CCNGG 4 cut(s) 28, 565, 658, 789
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 3 cut(s) 77, 142, 169
TaiI ACGT 3 cut(s) 183, 654, 782
TaqI TCGA 2 cut(s) 819, 828
TasI AATT 4 cut(s) 69, 508, 576, 642
TatI WGTACW 4 cut(s) 455, 681, 734, 798
TauI GCSGC 1 cut(s) 528
TfiI GAWTC 2 cut(s) 146, 821
Tru1I TTAA 2 cut(s) 38, 111
Tru9I TTAA 2 cut(s) 38, 111
TscAI CASTG 2 cut(s) 80, 547
TseFI GTSAC 5 cut(s) 163, 169, 542, 604, 878
TseI GCWGC 3 cut(s) 84, 154, 593
Tsp45I GTSAC 5 cut(s) 163, 169, 542, 604, 878
TspDTI ATGAA 5 cut(s) 303, 438, 641, 663, 735
TspRI CASTG 2 cut(s) 80, 547
Van91I CCANNNNNTGG 1 cut(s) 701
VpaK11BI GGWCC 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 654
ZrmI AGTACT 1 cut(s) 457
Zsp2I ATGCAT 1 cut(s) 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.