AT5G27820

ribosomal protein L18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009364
Physical Location & Seq
Forward (+)
9859801 .. 9861129
1329 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27820.1

Sequence Viewer

Length: 345 bp
ATGGTGATTCCACCAGCAGTGAGACCACCACGTCTCTTCGACTACCTAAAACCTTACGTGCTGAAAATGCATTTCACAAACAAGTTTGTCCACGCACAAGTCATCCACTCACCAACAGCCACAGTGGCTTGCTCAGCGAGCTCGCAAGAGAAAGCATTGAGAGAAACAATGGACATCACACGCGACGTCGCTGCTGCTTCAAAGATTGGTAAAATTCTTGGAGAAAGACTCTTGATGAAAGATATTCCCGCTGTTACTATTCAGATGAAGAAAGAACAGAGGTATCATGGTAAAGTCAAGGCTGTTATCGATTCTGTAAGAGAAGCTGGTGTCAAGTTGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

114

Amino Acids

12.74

Weight (kDa)

9.95

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 20 - 113 1.2e-14 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 30
AatII GACGTC 1 cut(s) 189
AccII CGCG 1 cut(s) 183
AciI CCGC 1 cut(s) 249
AcsI RAATTY 1 cut(s) 213
AcyI GRCGYC 1 cut(s) 186
AgsI TTSAA 1 cut(s) 201
AjiI CACGTC 1 cut(s) 32
AluBI AGCT 2 cut(s) 141, 326
AluI AGCT 2 cut(s) 141, 326
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 2 cut(s) 16, 38
ApeKI GCWGC 2 cut(s) 191, 194
ApoI RAATTY 1 cut(s) 213
AsuHPI GGTGA 2 cut(s) 16, 102
BaeI ACNNNNGTAYC 2 cut(s) 266, 299
BanII GRGCYC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 143
BbvI GCAGC 2 cut(s) 178, 181
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BcoDI GTCTC 2 cut(s) 16, 38
BglI GCCNNNNNGGC 1 cut(s) 125
BisI GCNGC 2 cut(s) 192, 195
BlpI GCTNAGC 1 cut(s) 133
BlsI GCNGC 2 cut(s) 193, 196
BmgBI CACGTC 1 cut(s) 32
BplI GAGNNNNNCTC 2 cut(s) 213, 245
Bpu1102I GCTNAGC 1 cut(s) 133
Bsa29I ATCGAT 1 cut(s) 309
BsaAI YACGTR 1 cut(s) 58
BsaHI GRCGYC 1 cut(s) 186
BsaI GGTCTC 1 cut(s) 16
BseCI ATCGAT 1 cut(s) 309
BseGI GGATG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 147
BseXI GCAGC 2 cut(s) 178, 181
Bsh1236I CGCG 1 cut(s) 183
BshVI ATCGAT 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 143
BsmAI GTCTC 2 cut(s) 16, 38
BsmBI CGTCTC 1 cut(s) 38
Bso31I GGTCTC 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 143
Bsp1720I GCTNAGC 1 cut(s) 133
BspACI CCGC 1 cut(s) 249
BspCNI CTCAG 1 cut(s) 146
BspDI ATCGAT 1 cut(s) 309
BspFNI CGCG 1 cut(s) 183
BspTNI GGTCTC 1 cut(s) 16
BssNI GRCGYC 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 124
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 58
BstC8I GCNNGC 3 cut(s) 130, 139, 143
BstDEI CTNAG 1 cut(s) 133
BstF5I GGATG 1 cut(s) 102
BstFNI CGCG 1 cut(s) 183
BstMAI GTCTC 2 cut(s) 16, 38
BstMWI GCNNNNNNNGC 4 cut(s) 67, 125, 134, 138
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 178, 181
Bsu15I ATCGAT 1 cut(s) 309
BsuTUI ATCGAT 1 cut(s) 309
BtrI CACGTC 1 cut(s) 32
BtsCI GGATG 1 cut(s) 102
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 2 cut(s) 24, 129
Cac8I GCNNGC 3 cut(s) 130, 139, 143
ClaI ATCGAT 1 cut(s) 309
CviAII CATG 1 cut(s) 287
CviJI RGCY 5 cut(s) 119, 128, 141, 302, 326
CviKI_1 RGCY 5 cut(s) 119, 128, 141, 302, 326
DdeI CTNAG 1 cut(s) 133
DrdI GACNNNNNNGTC 1 cut(s) 30
DseDI GACNNNNNNGTC 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Ecl136II GAGCTC 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 143
Eco31I GGTCTC 1 cut(s) 16
Eco53kI GAGCTC 1 cut(s) 141
EcoICRI GAGCTC 1 cut(s) 141
EcoT22I ATGCAT 1 cut(s) 72
EcoT38I GRGCYC 1 cut(s) 143
Esp3I CGTCTC 1 cut(s) 38
FaeI CATG 1 cut(s) 290
FaiI YATR 1 cut(s) 288
FatI CATG 1 cut(s) 286
FauI CCCGC 1 cut(s) 256
Fnu4HI GCNGC 2 cut(s) 192, 195
FokI GGATG 1 cut(s) 89
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 2 cut(s) 192, 195
GluI GCNGC 2 cut(s) 192, 195
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 1 cut(s) 290
HinfI GANTC 3 cut(s) 7, 228, 311
HphI GGTGA 2 cut(s) 16, 102
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 2 cut(s) 264, 344
Hpy188III TCNNGA 1 cut(s) 232
Hpy8I GTNNAC 1 cut(s) 91
Hpy99I CGWCG 2 cut(s) 188, 191
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4IV ACGT 3 cut(s) 31, 57, 186
HpyCH4V TGCA 1 cut(s) 70
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 125, 134, 138
HpyF3I CTNAG 1 cut(s) 133
HpySE526I ACGT 3 cut(s) 31, 57, 186
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 1 cut(s) 290
LpnPI CCDG 2 cut(s) 27, 312
Lsp1109I GCAGC 2 cut(s) 178, 181
MaeII ACGT 3 cut(s) 31, 57, 186
MaeIII GTNAC 1 cut(s) 253
MboII GAAGA 2 cut(s) 28, 280
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 1 cut(s) 213
MlyI GAGTC 1 cut(s) 222
MnlI CCTC 1 cut(s) 273
Mph1103I ATGCAT 1 cut(s) 72
MspA1I CMGCKG 1 cut(s) 251
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 4 cut(s) 67, 125, 134, 138
NlaIII CATG 1 cut(s) 290
NsiI ATGCAT 1 cut(s) 72
PfeI GAWTC 2 cut(s) 7, 311
PkrI GCNGC 2 cut(s) 193, 196
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
Ppu21I YACGTR 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 143
SacI GAGCTC 1 cut(s) 143
SatI GCNGC 2 cut(s) 192, 195
SchI GAGTC 1 cut(s) 222
SduI GDGCHC 1 cut(s) 143
SetI ASST 8 cut(s) 34, 48, 55, 60, 143, 189, 284, 328
Sse9I AATT 1 cut(s) 213
SsiI CCGC 1 cut(s) 249
SstI GAGCTC 1 cut(s) 143
TaaI ACNGT 1 cut(s) 124
TaiI ACGT 3 cut(s) 34, 60, 189
TaqI TCGA 2 cut(s) 39, 309
TasI AATT 1 cut(s) 213
TfiI GAWTC 2 cut(s) 7, 311
TscAI CASTG 2 cut(s) 24, 129
TseI GCWGC 2 cut(s) 191, 194
TspDTI ATGAA 2 cut(s) 251, 281
TspRI CASTG 2 cut(s) 24, 129
XapI RAATTY 1 cut(s) 213
ZraI GACGTC 1 cut(s) 187
Zsp2I ATGCAT 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.