MD12G1212100.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009364
Physical Location & Seq
Reverse (-)
29095084 .. 29096682
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1212100.v1.1.491

Sequence Viewer

Length: 279 bp
ATGCACTTCACAAACAAATATGTAAGCGCCCAGGTGATCCACACACCAACTGCCACTGTGGCATCTTCTGCAAGCTCCCAAGAAAAGGCCTTGAGAGAAAGCATGGAGATACGTCGGGATGTTGCTGCTGCTGCAAAGATTGGGAAGATATTAGGGGAGCGCCTGCTGCTCAAGAACATTCCCGCTGTATCCGTCCACTTGAAGAAAGAACAGAAATATCACGGTAAGGTTAAAGCTGTCATTGATAGTGTGGTTGAAGCAGGTGTCAAACTACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

93

Amino Acids

10.01

Weight (kDa)

9.93

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 4 - 91 2.8e-13 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 251
Acc36I ACCTGC 1 cut(s) 251
AciI CCGC 1 cut(s) 183
AclWI GGATC 1 cut(s) 31
AfiI CCNNNNNNNGG 1 cut(s) 85
AgsI TTSAA 2 cut(s) 202, 257
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 2 cut(s) 75, 236
AluI AGCT 2 cut(s) 75, 236
AlwI GGATC 1 cut(s) 31
AoxI GGCC 1 cut(s) 87
ApeKI GCWGC 4 cut(s) 125, 128, 131, 166
AspLEI GCGC 2 cut(s) 29, 162
AsuHPI GGTGA 1 cut(s) 46
BbvI GCAGC 4 cut(s) 112, 115, 118, 153
BciT130I CCWGG 1 cut(s) 32
BciVI GTATCC 1 cut(s) 199
BfoI RGCGCY 2 cut(s) 30, 163
BfuAI ACCTGC 1 cut(s) 251
BfuI GTATCC 1 cut(s) 199
BglI GCCNNNNNGGC 1 cut(s) 59
BisI GCNGC 4 cut(s) 126, 129, 132, 167
BlsI GCNGC 4 cut(s) 127, 130, 133, 168
Bme1390I CCNGG 1 cut(s) 32
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 1 cut(s) 71
BpuEI CTTGAG 2 cut(s) 112, 155
BsaJI CCNNGG 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 85
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 85
BseXI GCAGC 4 cut(s) 112, 115, 118, 153
BshFI GGCC 1 cut(s) 89
BslI CCNNNNNNNGG 1 cut(s) 85
BsnI GGCC 1 cut(s) 89
Bsp143I GATC 1 cut(s) 36
BspACI CCGC 1 cut(s) 183
BspANI GGCC 1 cut(s) 89
BspMI ACCTGC 1 cut(s) 251
BspPI GGATC 1 cut(s) 31
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 2 cut(s) 58, 224
BstAPI GCANNNNNTGC 1 cut(s) 68
BstC8I GCNNGC 2 cut(s) 73, 164
BstF5I GGATG 1 cut(s) 124
BstH2I RGCGCY 2 cut(s) 30, 163
BstHHI GCGC 2 cut(s) 29, 162
BstKTI GATC 1 cut(s) 39
BstMBI GATC 1 cut(s) 36
BstMWI GCNNNNNNNGC 4 cut(s) 59, 68, 131, 166
BstNI CCWGG 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 30
BstV1I GCAGC 4 cut(s) 112, 115, 118, 153
BsuI GTATCC 1 cut(s) 199
BsuRI GGCC 1 cut(s) 89
BtsCI GGATG 1 cut(s) 124
BtsIMutI CAGTG 1 cut(s) 54
BveI ACCTGC 1 cut(s) 251
Cac8I GCNNGC 2 cut(s) 73, 164
CfoI GCGC 2 cut(s) 29, 162
CviAII CATG 1 cut(s) 103
CviJI RGCY 3 cut(s) 75, 89, 236
CviKI_1 RGCY 3 cut(s) 75, 89, 236
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
Eco147I AGGCCT 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 30
FaeI CATG 1 cut(s) 106
FaiI YATR 2 cut(s) 21, 104
FatI CATG 1 cut(s) 102
FauI CCCGC 1 cut(s) 190
Fnu4HI GCNGC 4 cut(s) 126, 129, 132, 167
FokI GGATG 1 cut(s) 131
Fsp4HI GCNGC 4 cut(s) 126, 129, 132, 167
GlaI GCGC 2 cut(s) 28, 161
GluI GCNGC 4 cut(s) 126, 129, 132, 167
HaeII RGCGCY 2 cut(s) 30, 163
HaeIII GGCC 1 cut(s) 89
HhaI GCGC 2 cut(s) 29, 162
Hin1II CATG 1 cut(s) 106
Hin6I GCGC 2 cut(s) 27, 160
HinP1I GCGC 2 cut(s) 27, 160
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 1 cut(s) 278
Hpy188III TCNNGA 2 cut(s) 116, 172
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 1 cut(s) 117
HpyCH4III ACNGT 2 cut(s) 58, 224
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 3 cut(s) 4, 71, 134
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 68, 131, 166
HpySE526I ACGT 1 cut(s) 112
Hsp92II CATG 1 cut(s) 106
HspAI GCGC 2 cut(s) 27, 160
Kzo9I GATC 1 cut(s) 36
LmnI GCTCC 2 cut(s) 80, 157
LpnPI CCDG 4 cut(s) 17, 44, 176, 246
Lsp1109I GCAGC 4 cut(s) 112, 115, 118, 153
LweI GCATC 1 cut(s) 71
MaeII ACGT 1 cut(s) 112
MalI GATC 1 cut(s) 38
MboI GATC 1 cut(s) 36
MboII GAAGA 3 cut(s) 57, 157, 214
MseI TTAA 1 cut(s) 231
MspA1I CMGCKG 1 cut(s) 185
MspR9I CCNGG 1 cut(s) 32
MvaI CCWGG 1 cut(s) 32
MwoI GCNNNNNNNGC 4 cut(s) 59, 68, 131, 166
NdeII GATC 1 cut(s) 36
NlaIII CATG 1 cut(s) 106
PaqCI CACCTGC 1 cut(s) 251
PceI AGGCCT 1 cut(s) 89
PkrI GCNGC 4 cut(s) 127, 130, 133, 168
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 4 cut(s) 126, 129, 132, 167
Sau3AI GATC 1 cut(s) 36
ScrFI CCNGG 1 cut(s) 32
SetI ASST 6 cut(s) 36, 77, 115, 231, 238, 265
SfaNI GCATC 1 cut(s) 71
SmlI CTYRAG 2 cut(s) 91, 170
SmoI CTYRAG 2 cut(s) 91, 170
SseBI AGGCCT 1 cut(s) 89
SsiI CCGC 1 cut(s) 183
StuI AGGCCT 1 cut(s) 89
StyD4I CCNGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 58, 224
TaiI ACGT 1 cut(s) 115
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 1 cut(s) 61
TseI GCWGC 4 cut(s) 125, 128, 131, 166
TspGWI ACGGA 1 cut(s) 181
TspRI CASTG 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.