MD04G1198900.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009364
Physical Location & Seq
Reverse (-)
28623880 .. 28625567
1688 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1198900.v1.1.491

Sequence Viewer

Length: 279 bp
ATGCACTTTACAAACAAATACGTAAGTGCCCAGGTGATCCACACACCAACTGCCACTGTAGCCTCTTCTGCAAGCTCCCAGGAAAAGGCCTTGAGAGAAAGCATGGAGATACAGCGGGACGTTGCTGCTGCTGCAAAGATAGGGAAGATATTGGGGGAGCGTTTGCTGCTCAAAAACATTCCCGCTGTATCTGTCCACTTGAAGAAAGAACAGAAATATCACGGTAAGGTTAAAGCTGTCATTGACAGTGTGGTCGAAGCAGGTGTCAAACTACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

93

Amino Acids

9.98

Weight (kDa)

9.79

Isoelectric Point (pI)

34.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 4 - 91 2.5e-13 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 251
AasI GACNNNNNNGTC 1 cut(s) 251
Acc36I ACCTGC 1 cut(s) 251
AciI CCGC 2 cut(s) 115, 183
AclWI GGATC 1 cut(s) 31
AfiI CCNNNNNNNGG 1 cut(s) 85
AgsI TTSAA 1 cut(s) 202
AjnI CCWGG 2 cut(s) 30, 78
AluBI AGCT 2 cut(s) 75, 236
AluI AGCT 2 cut(s) 75, 236
AlwI GGATC 1 cut(s) 31
AoxI GGCC 1 cut(s) 87
ApeKI GCWGC 4 cut(s) 125, 128, 131, 166
ArsI GACNNNNNNTTYG 1 cut(s) 249
AsuHPI GGTGA 1 cut(s) 46
BaeGI GKGCMC 1 cut(s) 31
BbvI GCAGC 4 cut(s) 112, 115, 118, 153
BciT130I CCWGG 2 cut(s) 32, 80
BfmI CTRYAG 1 cut(s) 57
BfuAI ACCTGC 1 cut(s) 251
BisI GCNGC 4 cut(s) 126, 129, 132, 167
BlsI GCNGC 4 cut(s) 127, 130, 133, 168
Bme1390I CCNGG 2 cut(s) 32, 80
BmrFI CCNGG 2 cut(s) 32, 80
BpuEI CTTGAG 1 cut(s) 112
BsaAI YACGTR 1 cut(s) 22
BsaJI CCNNGG 2 cut(s) 30, 78
Bsc4I CCNNNNNNNGG 1 cut(s) 85
BseBI CCWGG 2 cut(s) 32, 80
BseDI CCNNGG 2 cut(s) 30, 78
BseLI CCNNNNNNNGG 1 cut(s) 85
BseSI GKGCMC 1 cut(s) 31
BseXI GCAGC 4 cut(s) 112, 115, 118, 153
BshFI GGCC 1 cut(s) 89
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 85
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 1 cut(s) 36
BspACI CCGC 2 cut(s) 115, 183
BspANI GGCC 1 cut(s) 89
BspMI ACCTGC 1 cut(s) 251
BspPI GGATC 1 cut(s) 31
BssECI CCNNGG 2 cut(s) 30, 78
BssMI GATC 1 cut(s) 36
Bst2UI CCWGG 2 cut(s) 32, 80
Bst4CI ACNGT 3 cut(s) 58, 224, 248
Bst6I CTCTTC 1 cut(s) 70
BstBAI YACGTR 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 73
BstKTI GATC 1 cut(s) 39
BstMBI GATC 1 cut(s) 36
BstMWI GCNNNNNNNGC 4 cut(s) 59, 68, 131, 166
BstNI CCWGG 2 cut(s) 32, 80
BstSCI CCNGG 2 cut(s) 30, 78
BstSFI CTRYAG 1 cut(s) 57
BstSLI GKGCMC 1 cut(s) 31
BstSNI TACGTA 1 cut(s) 22
BstV1I GCAGC 4 cut(s) 112, 115, 118, 153
BsuRI GGCC 1 cut(s) 89
BtsIMutI CAGTG 2 cut(s) 54, 253
BveI ACCTGC 1 cut(s) 251
Cac8I GCNNGC 1 cut(s) 73
CviAII CATG 1 cut(s) 103
CviJI RGCY 4 cut(s) 62, 75, 89, 236
CviKI_1 RGCY 4 cut(s) 62, 75, 89, 236
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Eco105I TACGTA 1 cut(s) 22
Eco147I AGGCCT 1 cut(s) 89
EcoRII CCWGG 2 cut(s) 30, 78
FaeI CATG 1 cut(s) 106
FaiI YATR 1 cut(s) 104
FaqI GGGAC 1 cut(s) 131
FatI CATG 1 cut(s) 102
FauI CCCGC 2 cut(s) 108, 190
Fnu4HI GCNGC 4 cut(s) 126, 129, 132, 167
Fsp4HI GCNGC 4 cut(s) 126, 129, 132, 167
GluI GCNGC 4 cut(s) 126, 129, 132, 167
HaeIII GGCC 1 cut(s) 89
Hin1II CATG 1 cut(s) 106
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 1 cut(s) 196
Hpy8I GTNNAC 1 cut(s) 196
HpyCH4III ACNGT 3 cut(s) 58, 224, 248
HpyCH4IV ACGT 2 cut(s) 21, 120
HpyCH4V TGCA 3 cut(s) 4, 71, 134
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 68, 131, 166
HpySE526I ACGT 2 cut(s) 21, 120
Hsp92II CATG 1 cut(s) 106
Kzo9I GATC 1 cut(s) 36
LmnI GCTCC 2 cut(s) 80, 157
LpnPI CCDG 5 cut(s) 17, 44, 65, 92, 246
Lsp1109I GCAGC 4 cut(s) 112, 115, 118, 153
MaeII ACGT 2 cut(s) 21, 120
MalI GATC 1 cut(s) 38
MboI GATC 1 cut(s) 36
MboII GAAGA 3 cut(s) 57, 157, 214
MhlI GDGCHC 1 cut(s) 31
MnlI CCTC 1 cut(s) 73
MseI TTAA 1 cut(s) 231
MspA1I CMGCKG 2 cut(s) 115, 185
MspR9I CCNGG 2 cut(s) 32, 80
MvaI CCWGG 2 cut(s) 32, 80
MwoI GCNNNNNNNGC 4 cut(s) 59, 68, 131, 166
NdeII GATC 1 cut(s) 36
NlaIII CATG 1 cut(s) 106
PaqCI CACCTGC 1 cut(s) 251
PceI AGGCCT 1 cut(s) 89
PkrI GCNGC 4 cut(s) 127, 130, 133, 168
Ppu21I YACGTR 1 cut(s) 22
Psp6I CCWGG 2 cut(s) 30, 78
PspGI CCWGG 2 cut(s) 30, 78
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 4 cut(s) 126, 129, 132, 167
Sau3AI GATC 1 cut(s) 36
ScrFI CCNGG 2 cut(s) 32, 80
SduI GDGCHC 1 cut(s) 31
SetI ASST 7 cut(s) 24, 36, 77, 123, 231, 238, 265
SfcI CTRYAG 1 cut(s) 57
SmlI CTYRAG 1 cut(s) 91
SmoI CTYRAG 1 cut(s) 91
SnaBI TACGTA 1 cut(s) 22
SseBI AGGCCT 1 cut(s) 89
SsiI CCGC 2 cut(s) 115, 183
StuI AGGCCT 1 cut(s) 89
StyD4I CCNGG 2 cut(s) 30, 78
TaaI ACNGT 3 cut(s) 58, 224, 248
TaiI ACGT 2 cut(s) 24, 123
TaqI TCGA 1 cut(s) 255
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 2 cut(s) 61, 253
TseI GCWGC 4 cut(s) 125, 128, 131, 166
TspRI CASTG 2 cut(s) 61, 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.