RLG00000025332

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0009364
Physical Location & Seq
Forward (+)
45092280 .. 45093647
1368 bp
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UTR
Exon/CDS
Intron
RLM00000025332

Sequence Viewer

Length: 423 bp
ATGAGGCCCAATTCTCTCCCTCCCGACTCCCAACACCTGAAAGAAAGCTTGGTTTGCGAGACATCCACTCCGACAGAGATGGTTGTTCCTCCTCCAGTCAGGCCACCTACTATTACGAAGTTTCTAAAGCCTTATGTCCTGAAAATGCACTTTACAAATAAATTTGTAACTGCACAGGTCATCCACTCGCCAACTGCCACTGTAGCCTCTTCCGCAAGCTCTCAGGAAAAGGCCTTGAGAGAAAGCTTGGAATCACATCGGGATGTTGCTGCTGCTGCAAAGATTGGGAAGATATTAGGTGAGCGCCTGCTGCTCAAGAACATCCCTGCTGTCGCAGTCCACTTGAAGAGAGAACAGAAATATCATGGTAAGGTTAAAGCTGTCATAGATAATGTGGTGGAAGCAGGGGTTAAGCTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

141

Amino Acids

15.35

Weight (kDa)

9.79

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 46 - 139 5.1e-13 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 213
AcsI RAATTY 1 cut(s) 161
AgsI TTSAA 1 cut(s) 346
AjuI GAANNNNNNNTTGG 2 cut(s) 32, 64
AluBI AGCT 5 cut(s) 48, 219, 246, 380, 415
AluI AGCT 5 cut(s) 48, 219, 246, 380, 415
Alw26I GTCTC 1 cut(s) 53
AoxI GGCC 3 cut(s) 5, 101, 231
ApeKI GCWGC 4 cut(s) 269, 272, 275, 310
ApoI RAATTY 1 cut(s) 161
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 1 cut(s) 6
AsuHPI GGTGA 1 cut(s) 311
BbvI GCAGC 4 cut(s) 256, 259, 262, 297
BccI CCATC 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 53
BfmI CTRYAG 1 cut(s) 201
BfoI RGCGCY 1 cut(s) 307
BisI GCNGC 4 cut(s) 270, 273, 276, 311
BlsI GCNGC 4 cut(s) 271, 274, 277, 312
BmgT120I GGNCC 1 cut(s) 6
BpmI CTGGAG 1 cut(s) 78
BpuEI CTTGAG 2 cut(s) 256, 299
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bse1I ACTGG 1 cut(s) 95
BseGI GGATG 4 cut(s) 62, 180, 268, 321
BseMII CTCAG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 95
BseRI GAGGAG 1 cut(s) 81
BseXI GCAGC 4 cut(s) 256, 259, 262, 297
BsgI GTGCAG 1 cut(s) 156
BshFI GGCC 3 cut(s) 7, 103, 233
BsmAI GTCTC 1 cut(s) 53
BsnI GGCC 3 cut(s) 7, 103, 233
BspACI CCGC 1 cut(s) 213
BspANI GGCC 3 cut(s) 7, 103, 233
BspCNI CTCAG 1 cut(s) 235
BsrI ACTGG 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 202
Bst6I CTCTTC 2 cut(s) 214, 341
BstC8I GCNNGC 2 cut(s) 217, 308
BstDEI CTNAG 1 cut(s) 222
BstF5I GGATG 4 cut(s) 62, 180, 268, 321
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 1 cut(s) 306
BstMAI GTCTC 1 cut(s) 53
BstMWI GCNNNNNNNGC 5 cut(s) 54, 203, 212, 275, 310
BstSFI CTRYAG 1 cut(s) 201
BstV1I GCAGC 4 cut(s) 256, 259, 262, 297
BsuRI GGCC 3 cut(s) 7, 103, 233
BtsCI GGATG 4 cut(s) 62, 180, 268, 321
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 2 cut(s) 217, 308
CfoI GCGC 1 cut(s) 306
Cfr13I GGNCC 1 cut(s) 6
CviAII CATG 1 cut(s) 365
DdeI CTNAG 1 cut(s) 222
Eam1104I CTCTTC 2 cut(s) 214, 341
EarI CTCTTC 2 cut(s) 214, 341
Eco147I AGGCCT 1 cut(s) 233
FaeI CATG 1 cut(s) 368
FaiI YATR 3 cut(s) 135, 366, 386
FatI CATG 1 cut(s) 364
Fnu4HI GCNGC 4 cut(s) 270, 273, 276, 311
FokI GGATG 4 cut(s) 49, 167, 275, 308
Fsp4HI GCNGC 4 cut(s) 270, 273, 276, 311
GlaI GCGC 1 cut(s) 305
GluI GCNGC 4 cut(s) 270, 273, 276, 311
GsuI CTGGAG 1 cut(s) 78
HaeII RGCGCY 1 cut(s) 307
HaeIII GGCC 3 cut(s) 7, 103, 233
HhaI GCGC 1 cut(s) 306
Hin1II CATG 1 cut(s) 368
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HindIII AAGCTT 2 cut(s) 46, 244
HinfI GANTC 2 cut(s) 26, 251
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 1 cut(s) 72
Hpy188III TCNNGA 5 cut(s) 23, 139, 224, 260, 316
Hpy8I GTNNAC 1 cut(s) 340
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4V TGCA 3 cut(s) 148, 173, 278
HpyF10VI GCNNNNNNNGC 5 cut(s) 54, 203, 212, 275, 310
HpyF3I CTNAG 1 cut(s) 222
Hsp92II CATG 1 cut(s) 368
HspAI GCGC 1 cut(s) 304
LpnPI CCDG 9 cut(s) 50, 85, 108, 152, 161, 209, 320, 339, 390
Lsp1109I GCAGC 4 cut(s) 256, 259, 262, 297
MaeIII GTNAC 1 cut(s) 166
MboII GAAGA 3 cut(s) 201, 301, 358
MluCI AATT 2 cut(s) 10, 161
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 95
MnlI CCTC 4 cut(s) 30, 99, 102, 217
MseI TTAA 2 cut(s) 375, 411
MslI CAYNNNNRTG 1 cut(s) 261
MwoI GCNNNNNNNGC 5 cut(s) 54, 203, 212, 275, 310
NlaIII CATG 1 cut(s) 368
PceI AGGCCT 1 cut(s) 233
PfeI GAWTC 1 cut(s) 251
PkrI GCNGC 4 cut(s) 271, 274, 277, 312
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
PspPI GGNCC 1 cut(s) 6
RseI CAYNNNNRTG 1 cut(s) 261
SaqAI TTAA 2 cut(s) 375, 411
SatI GCNGC 4 cut(s) 270, 273, 276, 311
Sau96I GGNCC 1 cut(s) 6
SchI GAGTC 1 cut(s) 20
SfcI CTRYAG 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 261
SmlI CTYRAG 2 cut(s) 235, 314
SmoI CTYRAG 2 cut(s) 235, 314
Sse9I AATT 2 cut(s) 10, 161
SseBI AGGCCT 1 cut(s) 233
SsiI CCGC 1 cut(s) 213
StuI AGGCCT 1 cut(s) 233
TaaI ACNGT 1 cut(s) 202
TasI AATT 2 cut(s) 10, 161
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 2 cut(s) 375, 411
Tru9I TTAA 2 cut(s) 375, 411
TscAI CASTG 1 cut(s) 205
TseI GCWGC 4 cut(s) 269, 272, 275, 310
TspRI CASTG 1 cut(s) 205
XapI RAATTY 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.