Rorug06G0057400

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009364
Physical Location & Seq
Forward (+)
7784461 .. 7785481
1021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0057400.1

Sequence Viewer

Length: 333 bp
ATGAAAATGCATGGAGAAGCGAATAGTAGAACACTAATTTTGGTCCTCTTTCTCGCCTTGCTCATCTGTTCTGGCTCGGTTCACTCGTGCGACTTCTCAAGCTCTCCTTCGCCTTCACCGGCCCTTGGGATGCCAACACCCCACGAGCCATCTAAAGGGATTTTCAAAAAGAAAAGGAGAGGGTCTTTCCCTGCGACTTGTTACTCAAAATGTAATCAGTGCGAGCCTTGCATGCCAGTTCAAGTATCAGTCCGGGCAATGGAATTGGAAGAAAACGAGTACTATCCACTAGTCTGGAAATGTGCTTGTGGTGATAATATATTTTCCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

110

Amino Acids

12.12

Weight (kDa)

8.13

Isoelectric Point (pI)

85.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 60 - 110 3.2e-20 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 3 cut(s) 125, 155, 259
AgsI TTSAA 2 cut(s) 166, 242
AhlI ACTAGT 1 cut(s) 289
AluBI AGCT 1 cut(s) 102
AluI AGCT 1 cut(s) 102
AoxI GGCC 1 cut(s) 120
AspS9I GGNCC 2 cut(s) 43, 121
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 2 cut(s) 108, 323
AvaII GGWCC 1 cut(s) 43
BauI CACGAG 2 cut(s) 85, 143
BccI CCATC 1 cut(s) 157
BcnI CCSGG 1 cut(s) 254
BcuI ACTAGT 1 cut(s) 289
BfaI CTAG 1 cut(s) 290
BmcAI AGTACT 1 cut(s) 281
Bme1390I CCNGG 1 cut(s) 254
Bme18I GGWCC 1 cut(s) 43
BmgT120I GGNCC 2 cut(s) 43, 121
BmrFI CCNGG 1 cut(s) 254
BmsI GCATC 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 82
BpuMI CCSGG 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 124
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 155, 259
Bse118I RCCGGY 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 236
Bse3DI GCAATG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 124
BseGI GGATG 1 cut(s) 135
BseLI CCNNNNNNNGG 3 cut(s) 125, 155, 259
BseMI GCAATG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 236
BshFI GGCC 1 cut(s) 122
BsiSI CCGG 2 cut(s) 119, 253
BslI CCNNNNNNNGG 3 cut(s) 125, 155, 259
BsnI GGCC 1 cut(s) 122
BspANI GGCC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 264
BsrFI RCCGGY 1 cut(s) 118
BsrI ACTGG 1 cut(s) 236
BssAI RCCGGY 1 cut(s) 118
BssECI CCNNGG 1 cut(s) 124
BssSI CACGAG 2 cut(s) 85, 143
BssT1I CCWWGG 1 cut(s) 124
Bst2BI CACGAG 2 cut(s) 85, 143
BstC8I GCNNGC 2 cut(s) 224, 233
BstDEI CTNAG 1 cut(s) 330
BstF5I GGATG 1 cut(s) 135
BstMWI GCNNNNNNNGC 2 cut(s) 228, 232
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 252
BstXI CCANNNNNNTGG 1 cut(s) 294
BsuRI GGCC 1 cut(s) 122
BtsCI GGATG 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 224
Cac8I GCNNGC 2 cut(s) 224, 233
Cfr10I RCCGGY 1 cut(s) 118
Cfr13I GGNCC 2 cut(s) 43, 121
Csp6I GTAC 1 cut(s) 280
CviAII CATG 2 cut(s) 11, 232
CviJI RGCY 5 cut(s) 75, 102, 122, 148, 226
CviKI_1 RGCY 5 cut(s) 75, 102, 122, 148, 226
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 1 cut(s) 330
Eco130I CCWWGG 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 124
EcoT22I ATGCAT 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 124
FaeI CATG 2 cut(s) 14, 235
FaiI YATR 3 cut(s) 12, 233, 320
FalI AAGNNNNNCTT 2 cut(s) 91, 123
FatI CATG 2 cut(s) 10, 231
FokI GGATG 1 cut(s) 142
FspBI CTAG 1 cut(s) 290
HaeIII GGCC 1 cut(s) 122
HapII CCGG 2 cut(s) 119, 253
Hin1II CATG 2 cut(s) 14, 235
HpaII CCGG 2 cut(s) 119, 253
HphI GGTGA 2 cut(s) 108, 323
Hpy166II GTNNAC 1 cut(s) 82
Hpy188III TCNNGA 1 cut(s) 295
Hpy8I GTNNAC 1 cut(s) 82
HpyAV CCTTC 2 cut(s) 117, 123
HpyCH4V TGCA 2 cut(s) 10, 231
HpyF10VI GCNNNNNNNGC 2 cut(s) 228, 232
HpyF3I CTNAG 1 cut(s) 330
Hsp92II CATG 2 cut(s) 14, 235
LpnPI CCDG 6 cut(s) 57, 132, 204, 249, 266, 280
LweI GCATC 1 cut(s) 120
MaeI CTAG 1 cut(s) 290
MaeIII GTNAC 1 cut(s) 200
MboII GAAGA 1 cut(s) 281
MluCI AATT 2 cut(s) 36, 263
MnlI CCTC 2 cut(s) 56, 173
Mph1103I ATGCAT 1 cut(s) 12
MspI CCGG 2 cut(s) 119, 253
MspR9I CCNGG 1 cut(s) 254
MwoI GCNNNNNNNGC 2 cut(s) 228, 232
NciI CCSGG 1 cut(s) 254
NlaIII CATG 2 cut(s) 14, 235
NsiI ATGCAT 1 cut(s) 12
NspI RCATGY 1 cut(s) 235
PaeI GCATGC 1 cut(s) 235
PcsI WCGNNNNNNNCGW 1 cut(s) 83
PspPI GGNCC 2 cut(s) 43, 121
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
Sau96I GGNCC 2 cut(s) 43, 121
ScaI AGTACT 1 cut(s) 281
ScrFI CCNGG 1 cut(s) 254
SetI ASST 1 cut(s) 104
SfaNI GCATC 1 cut(s) 120
SinI GGWCC 1 cut(s) 43
SmlI CTYRAG 1 cut(s) 97
SmoI CTYRAG 1 cut(s) 97
SpeI ACTAGT 1 cut(s) 289
SphI GCATGC 1 cut(s) 235
Sse9I AATT 2 cut(s) 36, 263
SspMI CTAG 1 cut(s) 290
StyD4I CCNGG 1 cut(s) 252
StyI CCWWGG 1 cut(s) 124
TasI AATT 2 cut(s) 36, 263
TatI WGTACW 1 cut(s) 279
TscAI CASTG 1 cut(s) 224
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 43
XceI RCATGY 1 cut(s) 235
XspI CTAG 1 cut(s) 290
ZrmI AGTACT 1 cut(s) 281
Zsp2I ATGCAT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.