Rw3G005980

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0009364
Physical Location & Seq
Reverse (-)
5314074 .. 5314418
345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G005980.1

Sequence Viewer

Length: 345 bp
ATGGTTGTTCCTCCTCCAATCAGGCCACCTACTGTTACGAAGTTTCTAAAGCCTTATGTCCTGAAAATGCACTTTACAAATAAATTTGTAACTGCTCAGGTCATCCACTCGCCAACTGCCACTGTGGCCTCTTCCGCAAGCTCTCAGGAAAAGACCTTGAGAGAAAGCTTGGAATCGCATCGGGATGTTGCTGCTGCTGCAAAGATTGGGAAGATATTAGGTGCGCGCCTGCTGCTCAAGAGCATCCCTGCGGTCGCAGTCCACTTGAAGAGAGAACAGAAATATCATGGTAAGGTTAAAGCTGTCATAGATAATGTGGTGGAAGCAGGTGTTAAGCTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

114

Amino Acids

12.41

Weight (kDa)

10.29

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 20 - 113 2.6e-12 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 317
Acc36I ACCTGC 1 cut(s) 317
AccII CGCG 1 cut(s) 226
AciI CCGC 2 cut(s) 135, 251
AcsI RAATTY 1 cut(s) 83
AgsI TTSAA 1 cut(s) 268
AluBI AGCT 4 cut(s) 141, 168, 302, 337
AluI AGCT 4 cut(s) 141, 168, 302, 337
AoxI GGCC 2 cut(s) 23, 126
ApeKI GCWGC 4 cut(s) 191, 194, 197, 232
ApoI RAATTY 1 cut(s) 83
AspLEI GCGC 2 cut(s) 226, 228
BbvI GCAGC 4 cut(s) 178, 181, 184, 219
BfuAI ACCTGC 1 cut(s) 317
BglI GCCNNNNNGGC 1 cut(s) 125
BisI GCNGC 4 cut(s) 192, 195, 198, 233
BlsI GCNGC 4 cut(s) 193, 196, 199, 234
BmsI GCATC 2 cut(s) 187, 252
Bpu10I CCTNAGC 1 cut(s) 96
BpuEI CTTGAG 2 cut(s) 178, 221
BseGI GGATG 3 cut(s) 102, 190, 243
BseMII CTCAG 2 cut(s) 110, 158
BsePI GCGCGC 1 cut(s) 224
BseXI GCAGC 4 cut(s) 178, 181, 184, 219
Bsh1236I CGCG 1 cut(s) 226
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 2 cut(s) 25, 128
BsiEI CGRYCG 1 cut(s) 255
BsnI GGCC 2 cut(s) 25, 128
BspACI CCGC 2 cut(s) 135, 251
BspANI GGCC 2 cut(s) 25, 128
BspCNI CTCAG 2 cut(s) 109, 157
BspFNI CGCG 1 cut(s) 226
BspMI ACCTGC 1 cut(s) 317
BssHII GCGCGC 1 cut(s) 224
Bst4CI ACNGT 2 cut(s) 34, 124
Bst6I CTCTTC 2 cut(s) 136, 263
BstC8I GCNNGC 3 cut(s) 139, 226, 230
BstDEI CTNAG 2 cut(s) 96, 144
BstF5I GGATG 3 cut(s) 102, 190, 243
BstFNI CGCG 1 cut(s) 226
BstHHI GCGC 2 cut(s) 226, 228
BstMCI CGRYCG 1 cut(s) 255
BstMWI GCNNNNNNNGC 4 cut(s) 125, 134, 197, 232
BstUI CGCG 1 cut(s) 226
BstV1I GCAGC 4 cut(s) 178, 181, 184, 219
BsuRI GGCC 2 cut(s) 25, 128
BtsCI GGATG 3 cut(s) 102, 190, 243
BtsIMutI CAGTG 1 cut(s) 120
BveI ACCTGC 1 cut(s) 317
Cac8I GCNNGC 3 cut(s) 139, 226, 230
CfoI GCGC 2 cut(s) 226, 228
CviAII CATG 1 cut(s) 287
CviJI RGCY 7 cut(s) 25, 52, 128, 141, 168, 302, 337
CviKI_1 RGCY 7 cut(s) 25, 52, 128, 141, 168, 302, 337
DdeI CTNAG 2 cut(s) 96, 144
Eam1104I CTCTTC 2 cut(s) 136, 263
EarI CTCTTC 2 cut(s) 136, 263
FaeI CATG 1 cut(s) 290
FaiI YATR 3 cut(s) 57, 288, 308
FatI CATG 1 cut(s) 286
Fnu4HI GCNGC 4 cut(s) 192, 195, 198, 233
FokI GGATG 3 cut(s) 89, 197, 230
Fsp4HI GCNGC 4 cut(s) 192, 195, 198, 233
GlaI GCGC 2 cut(s) 225, 227
GluI GCNGC 4 cut(s) 192, 195, 198, 233
HaeIII GGCC 2 cut(s) 25, 128
HhaI GCGC 2 cut(s) 226, 228
Hin1II CATG 1 cut(s) 290
Hin6I GCGC 2 cut(s) 224, 226
HinP1I GCGC 2 cut(s) 224, 226
HindIII AAGCTT 1 cut(s) 166
HinfI GANTC 1 cut(s) 173
Hpy166II GTNNAC 1 cut(s) 262
Hpy188III TCNNGA 4 cut(s) 61, 146, 182, 238
Hpy8I GTNNAC 1 cut(s) 262
HpyCH4III ACNGT 2 cut(s) 34, 124
HpyCH4V TGCA 2 cut(s) 70, 200
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 134, 197, 232
HpyF3I CTNAG 2 cut(s) 96, 144
Hsp92II CATG 1 cut(s) 290
HspAI GCGC 2 cut(s) 224, 226
LpnPI CCDG 7 cut(s) 7, 74, 83, 131, 242, 261, 312
Lsp1109I GCAGC 4 cut(s) 178, 181, 184, 219
LweI GCATC 2 cut(s) 187, 252
MaeIII GTNAC 2 cut(s) 34, 88
MboII GAAGA 3 cut(s) 123, 223, 280
MluCI AATT 1 cut(s) 83
MnlI CCTC 3 cut(s) 21, 24, 139
MseI TTAA 2 cut(s) 297, 333
MslI CAYNNNNRTG 1 cut(s) 183
MvnI CGCG 1 cut(s) 226
MwoI GCNNNNNNNGC 4 cut(s) 125, 134, 197, 232
NlaIII CATG 1 cut(s) 290
PaqCI CACCTGC 1 cut(s) 317
PauI GCGCGC 1 cut(s) 224
PfeI GAWTC 1 cut(s) 173
PkrI GCNGC 4 cut(s) 193, 196, 199, 234
PteI GCGCGC 1 cut(s) 224
RseI CAYNNNNRTG 1 cut(s) 183
SaqAI TTAA 2 cut(s) 297, 333
SatI GCNGC 4 cut(s) 192, 195, 198, 233
SfaNI GCATC 2 cut(s) 187, 252
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 2 cut(s) 157, 236
SmoI CTYRAG 2 cut(s) 157, 236
Sse9I AATT 1 cut(s) 83
SsiI CCGC 2 cut(s) 135, 251
TaaI ACNGT 2 cut(s) 34, 124
TasI AATT 1 cut(s) 83
TfiI GAWTC 1 cut(s) 173
Tru1I TTAA 2 cut(s) 297, 333
Tru9I TTAA 2 cut(s) 297, 333
TscAI CASTG 1 cut(s) 127
TseI GCWGC 4 cut(s) 191, 194, 197, 232
TspRI CASTG 1 cut(s) 127
XapI RAATTY 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.