AT5G27940

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010075
Physical Location & Seq
Forward (+)
9974411 .. 9975203
793 bp
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UTR
Exon/CDS
Intron
AT5G27940.1

Sequence Viewer

Length: 474 bp
ATGGCAGAAACCGCCGATACAATCAACACCACCGTCTCAACGCCGCAGCCACAATTAGAAAGCAGATCAGACGAGACAAGTTGCCTCCAAAAACACAGATCTGACGCAACATCAGAGGTGACGAAAGAAGAGAAATCTGGAGGAATCTTGTTCAGCGTTTGGCCACCGTGTCAGAAAAGTCGCGACTACGTGGTGAATAGTATGATCAAGACCTTATCCACAGACTCGATCCTCTCCTACAAATACGGAACGATTAAACCTGAGGAAGCCTCCGCCGTCGCGAAATCTATAGAGGAAAAGGCTTACGACATCGCATCAAGGTTCGTGTCGAGTGATGGGATTAAGAATCTTGAGGTCTATGGCATAGAGACTAGCGAGCGTATGATTGAATCTGCGGAGGTTCGATTCAAAGCCAATGGAAGTATGGAACTACTACTAAATCAAACAATAAAGATGATGCAGTTGCTGATTTAG

Protein Analysis

157

Amino Acids

17.45

Weight (kDa)

5.02

Isoelectric Point (pI)

47.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WPP PF13943 51 - 136 5.1e-25 WPP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47200 AT5G27940 AT5G43070
fragaria_vesca FvH4_2g33670 FvH4_2g33670
malus_domestica MD08G1091600.v1.1 MD15G1075600.v1.1
prunus_persica Prupe.1G429000_v2.0.a1
pyrus_communis pycom08g07390 pycom15g07080
rosa_chinensis RchiOBHm_Chr6g0308881
rosa_laevigata RLG00000010608
rosa_multiflora Rmu_sc0004046.1_g000014
rosa_roxburghii Rroxscaffold_7G00159820
rosa_rugosa Rorug06G0368900 Rorug06G0369000
rosa_samantha Rh6AG480500 Rh6BG490000 Rh6CG495400 Rh6DG480800
rosa_wichuraiana Rw6G041880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 183, 281
AciI CCGC 4 cut(s) 12, 44, 273, 395
AclWI GGATC 1 cut(s) 223
AcoI YGGCCR 1 cut(s) 161
AgsI TTSAA 2 cut(s) 389, 409
Alw26I GTCTC 3 cut(s) 40, 68, 362
AlwI GGATC 1 cut(s) 223
AlwNI CAGNNNCTG 1 cut(s) 466
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 1 cut(s) 46
AsuHPI GGTGA 2 cut(s) 130, 205
AxyI CCTNAGG 1 cut(s) 261
BalI TGGCCA 1 cut(s) 163
BbvI GCAGC 1 cut(s) 58
BccI CCATC 1 cut(s) 329
BceAI ACGGC 1 cut(s) 260
BclI TGATCA 1 cut(s) 204
BcoDI GTCTC 3 cut(s) 40, 68, 362
BfaI CTAG 1 cut(s) 372
BfmI CTRYAG 1 cut(s) 288
BglII AGATCT 1 cut(s) 98
BisI GCNGC 2 cut(s) 44, 47
BlsI GCNGC 2 cut(s) 45, 48
BmsI GCATC 2 cut(s) 323, 447
BplI GAGNNNNNCTC 2 cut(s) 254, 286
BpmI CTGGAG 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 371
BsaAI YACGTR 1 cut(s) 190
Bse21I CCTNAGG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 252
BseXI GCAGC 1 cut(s) 58
Bsh1236I CGCG 2 cut(s) 183, 281
BshFI GGCC 1 cut(s) 163
BsmAI GTCTC 3 cut(s) 40, 68, 362
BsmBI CGTCTC 1 cut(s) 40
BsnI GGCC 1 cut(s) 163
Bsp143I GATC 4 cut(s) 65, 98, 204, 228
Bsp68I TCGCGA 2 cut(s) 183, 281
BspACI CCGC 4 cut(s) 12, 44, 273, 395
BspANI GGCC 1 cut(s) 163
BspCNI CTCAG 1 cut(s) 253
BspFNI CGCG 2 cut(s) 183, 281
BspPI GGATC 1 cut(s) 223
BssMI GATC 4 cut(s) 65, 98, 204, 228
Bst4CI ACNGT 2 cut(s) 34, 168
Bst6I CTCTTC 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 190
BstC8I GCNNGC 1 cut(s) 377
BstDEI CTNAG 1 cut(s) 261
BstFNI CGCG 2 cut(s) 183, 281
BstKTI GATC 4 cut(s) 68, 101, 207, 231
BstMAI GTCTC 3 cut(s) 40, 68, 362
BstMBI GATC 4 cut(s) 65, 98, 204, 228
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstSFI CTRYAG 1 cut(s) 288
BstUI CGCG 2 cut(s) 183, 281
BstV1I GCAGC 1 cut(s) 58
BstX2I RGATCY 1 cut(s) 98
BstYI RGATCY 1 cut(s) 98
Bsu36I CCTNAGG 1 cut(s) 261
BsuRI GGCC 1 cut(s) 163
BtgZI GCGATG 1 cut(s) 295
BtuMI TCGCGA 2 cut(s) 183, 281
Cac8I GCNNGC 1 cut(s) 377
CaiI CAGNNNCTG 1 cut(s) 466
CseI GACGC 1 cut(s) 113
CviJI RGCY 5 cut(s) 49, 163, 269, 302, 413
CviKI_1 RGCY 5 cut(s) 49, 163, 269, 302, 413
DdeI CTNAG 1 cut(s) 261
DpnI GATC 4 cut(s) 67, 100, 206, 230
DpnII GATC 4 cut(s) 65, 98, 204, 228
EaeI YGGCCR 1 cut(s) 161
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
EciI GGCGGA 1 cut(s) 262
Eco81I CCTNAGG 1 cut(s) 261
Esp3I CGTCTC 1 cut(s) 40
FaiI YATR 6 cut(s) 203, 290, 360, 365, 383, 425
FbaI TGATCA 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 44, 47
Fsp4HI GCNGC 2 cut(s) 44, 47
FspBI CTAG 1 cut(s) 372
GluI GCNGC 2 cut(s) 44, 47
GsuI CTGGAG 1 cut(s) 159
HaeIII GGCC 1 cut(s) 163
HgaI GACGC 1 cut(s) 113
HinfI GANTC 5 cut(s) 144, 224, 346, 389, 405
HphI GGTGA 2 cut(s) 130, 205
Hpy188I TCNGA 4 cut(s) 70, 103, 115, 174
Hpy188III TCNNGA 5 cut(s) 138, 182, 208, 280, 350
Hpy99I CGWCG 1 cut(s) 281
HpyCH4III ACNGT 2 cut(s) 34, 168
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 1 cut(s) 460
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 261
HpySE526I ACGT 1 cut(s) 189
Ksp22I TGATCA 1 cut(s) 204
Kzo9I GATC 4 cut(s) 65, 98, 204, 228
LpnPI CCDG 2 cut(s) 123, 273
Lsp1109I GCAGC 1 cut(s) 58
LweI GCATC 2 cut(s) 323, 447
MaeI CTAG 1 cut(s) 372
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 1 cut(s) 118
MalI GATC 4 cut(s) 67, 100, 206, 230
MboI GATC 4 cut(s) 65, 98, 204, 228
MboII GAAGA 1 cut(s) 140
MflI RGATCY 1 cut(s) 98
MlsI TGGCCA 1 cut(s) 163
MluCI AATT 1 cut(s) 53
MluNI TGGCCA 1 cut(s) 163
MlyI GAGTC 1 cut(s) 218
MnlI CCTC 9 cut(s) 95, 109, 134, 242, 256, 280, 286, 346, 391
Mox20I TGGCCA 1 cut(s) 163
MscI TGGCCA 1 cut(s) 163
MseI TTAA 2 cut(s) 255, 342
Msp20I TGGCCA 1 cut(s) 163
MvnI CGCG 2 cut(s) 183, 281
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeII GATC 4 cut(s) 65, 98, 204, 228
NmuCI GTSAC 1 cut(s) 118
NruI TCGCGA 2 cut(s) 183, 281
PfeI GAWTC 4 cut(s) 144, 346, 389, 405
PkrI GCNGC 2 cut(s) 45, 48
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Ppu21I YACGTR 1 cut(s) 190
PstNI CAGNNNCTG 1 cut(s) 466
PsuI RGATCY 1 cut(s) 98
RruI TCGCGA 2 cut(s) 183, 281
SaqAI TTAA 2 cut(s) 255, 342
SatI GCNGC 2 cut(s) 44, 47
Sau3AI GATC 4 cut(s) 65, 98, 204, 228
SchI GAGTC 1 cut(s) 218
SetI ASST 7 cut(s) 120, 192, 215, 262, 323, 357, 402
SfaNI GCATC 2 cut(s) 323, 447
SfcI CTRYAG 1 cut(s) 288
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 1 cut(s) 53
SsiI CCGC 4 cut(s) 12, 44, 273, 395
SspMI CTAG 1 cut(s) 372
TaaI ACNGT 2 cut(s) 34, 168
TaiI ACGT 1 cut(s) 192
TaqI TCGA 3 cut(s) 227, 329, 403
TasI AATT 1 cut(s) 53
TauI GCSGC 1 cut(s) 46
TfiI GAWTC 4 cut(s) 144, 346, 389, 405
Tru1I TTAA 2 cut(s) 255, 342
Tru9I TTAA 2 cut(s) 255, 342
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 1 cut(s) 46
Tsp45I GTSAC 1 cut(s) 118
TspGWI ACGGA 1 cut(s) 261
XcmI CCANNNNNNNNNTGG 1 cut(s) 421
XspI CTAG 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.