FvH4_2g33670

MFP1 attachment factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010075
Physical Location & Seq
Forward (+)
25187201 .. 25188191
991 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33670.t1

Sequence Viewer

Length: 555 bp
ATGTCTGACACCGAGAGCACCGTTGAGGTCGAATCCCAAGAAGTACCCAAGCGGGAGTCACAGGATCGGAAGCCGACCTCCGCCGCCGTCAACTACAGCATCTGGCCGCCGACCGAGCGCACCCGTAACGCAGTCGTCGACCGCCTCATCGAGACCCTCACCACTCCCTCCCCTCTCTCCCGACGCTACGGCACCTTGTCCGCCGACGAGGCAGGCCCCCAGGCCCGCCTCATCGAGCAGGACGCCTTCGCCGCCGCCGGTGGCTCCGCCGCCACGGAGGTCGGCAGTGAGACCGGGATGAAGATCCTGCAGATCTACTCCAAGGAGATCAGCAAGCGCATGCTCGATGTCGTCAAGGCTAAGAACGCCGCTGCTTCTGCTGCTGACAACGGCGCTTCCGAGACCGCCGAGACTGGTAGCCCCGCCGCCGCCGTGGAGGACGATGCCGCCGCCGCTGTTCCCGGTGAGGAGGTGAAGTCTGAGGAGGTGAAGACTGAGGAATCGAAGACTGAGGAGTCGAAGACTGAGGAGTCGAAGACTGAGGAGTCGACCTGA

Protein Analysis

185

Amino Acids

19.51

Weight (kDa)

4.51

Isoelectric Point (pI)

67.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WPP PF13943 33 - 127 1.2e-33 WPP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47200 AT5G27940 AT5G43070
fragaria_vesca FvH4_2g33670 FvH4_2g33670
malus_domestica MD08G1091600.v1.1 MD15G1075600.v1.1
prunus_persica Prupe.1G429000_v2.0.a1
pyrus_communis pycom08g07390 pycom15g07080
rosa_chinensis RchiOBHm_Chr6g0308881
rosa_laevigata RLG00000010608
rosa_multiflora Rmu_sc0004046.1_g000014
rosa_roxburghii Rroxscaffold_7G00159820
rosa_rugosa Rorug06G0368900 Rorug06G0369000
rosa_samantha Rh6AG480500 Rh6BG490000 Rh6CG495400 Rh6DG480800
rosa_wichuraiana Rw6G041880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 514, 529, 544
AccB1I GGYRCC 1 cut(s) 191
AccI GTMKAC 2 cut(s) 138, 548
AclWI GGATC 2 cut(s) 72, 298
AcoI YGGCCR 1 cut(s) 104
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 1 cut(s) 45
AjnI CCWGG 1 cut(s) 219
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 4 cut(s) 146, 284, 395, 404
AlwI GGATC 2 cut(s) 72, 298
AlwNI CAGNNNCTG 1 cut(s) 102
AoxI GGCC 3 cut(s) 104, 214, 222
ApeKI GCWGC 2 cut(s) 371, 380
AspLEI GCGC 3 cut(s) 120, 339, 395
AspS9I GGNCC 2 cut(s) 215, 223
AsuC2I CCSGG 2 cut(s) 295, 462
AsuHPI GGTGA 4 cut(s) 151, 476, 484, 499
BanI GGYRCC 1 cut(s) 191
BbsI GAAGAC 4 cut(s) 497, 512, 527, 542
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 2 cut(s) 358, 367
BceAI ACGGC 4 cut(s) 71, 205, 406, 416
BciT130I CCWGG 1 cut(s) 221
BcnI CCSGG 2 cut(s) 295, 462
BcoDI GTCTC 4 cut(s) 146, 284, 395, 404
BfmI CTRYAG 2 cut(s) 94, 308
BfoI RGCGCY 1 cut(s) 396
BglI GCCNNNNNGGC 1 cut(s) 209
BglII AGATCT 1 cut(s) 312
Bme1390I CCNGG 3 cut(s) 221, 295, 462
BmgT120I GGNCC 2 cut(s) 215, 223
BmiI GGNNCC 3 cut(s) 193, 217, 265
BmrFI CCNGG 3 cut(s) 221, 295, 462
BmsI GCATC 2 cut(s) 108, 433
BpiI GAAGAC 4 cut(s) 497, 512, 527, 542
BpuMI CCSGG 2 cut(s) 295, 462
BsaBI GATNNNNATC 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 243
BsaI GGTCTC 3 cut(s) 146, 284, 395
BsaJI CCNNGG 4 cut(s) 219, 273, 321, 432
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 1 cut(s) 418
Bse8I GATNNNNATC 1 cut(s) 302
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 4 cut(s) 219, 273, 321, 432
BseGI GGATG 1 cut(s) 303
BseJI GATNNNNATC 1 cut(s) 302
BseMII CTCAG 5 cut(s) 471, 486, 501, 516, 531
BseNI ACTGG 1 cut(s) 418
BseRI GAGGAG 4 cut(s) 482, 497, 527, 542
BseXI GCAGC 2 cut(s) 358, 367
Bsh1285I CGRYCG 2 cut(s) 114, 142
BshFI GGCC 3 cut(s) 106, 216, 224
BshNI GGYRCC 1 cut(s) 191
BsiEI CGRYCG 2 cut(s) 114, 142
BsiHKAI GWGCWC 1 cut(s) 20
BsiSI CCGG 3 cut(s) 258, 294, 462
BsmAI GTCTC 4 cut(s) 146, 284, 395, 404
BsnI GGCC 3 cut(s) 106, 216, 224
Bso31I GGTCTC 3 cut(s) 146, 284, 395
Bsp1286I GDGCHC 1 cut(s) 20
Bsp143I GATC 4 cut(s) 64, 303, 312, 327
BspANI GGCC 3 cut(s) 106, 216, 224
BspCNI CTCAG 5 cut(s) 472, 487, 502, 517, 532
BspLI GGNNCC 3 cut(s) 193, 217, 265
BspMAI CTGCAG 1 cut(s) 312
BspPI GGATC 2 cut(s) 72, 298
BspT107I GGYRCC 1 cut(s) 191
BspTNI GGTCTC 3 cut(s) 146, 284, 395
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 1 cut(s) 418
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 4 cut(s) 219, 273, 321, 432
BssMI GATC 4 cut(s) 64, 303, 312, 327
BssNI GRCGYC 1 cut(s) 243
BssT1I CCWWGG 1 cut(s) 321
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 22
BstACI GRCGYC 1 cut(s) 243
BstC8I GCNNGC 4 cut(s) 214, 226, 335, 341
BstDEI CTNAG 6 cut(s) 360, 480, 495, 510, 525, 540
BstDSI CCRYGG 2 cut(s) 273, 432
BstF5I GGATG 1 cut(s) 303
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 3 cut(s) 120, 339, 395
BstKTI GATC 4 cut(s) 67, 306, 315, 330
BstMAI GTCTC 4 cut(s) 146, 284, 395, 404
BstMBI GATC 4 cut(s) 64, 303, 312, 327
BstMCI CGRYCG 2 cut(s) 114, 142
BstMWI GCNNNNNNNGC 7 cut(s) 115, 209, 251, 365, 377, 380, 452
BstNI CCWGG 1 cut(s) 221
BstNSI RCATGY 1 cut(s) 343
BstSCI CCNGG 3 cut(s) 219, 293, 460
BstSFI CTRYAG 2 cut(s) 94, 308
BstV1I GCAGC 2 cut(s) 358, 367
BstV2I GAAGAC 4 cut(s) 497, 512, 527, 542
BstX2I RGATCY 2 cut(s) 303, 312
BstYI RGATCY 2 cut(s) 303, 312
BsuRI GGCC 3 cut(s) 106, 216, 224
BtgI CCRYGG 2 cut(s) 273, 432
BtsCI GGATG 1 cut(s) 303
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 1 cut(s) 292
Cac8I GCNNGC 4 cut(s) 214, 226, 335, 341
CaiI CAGNNNCTG 1 cut(s) 102
CfoI GCGC 3 cut(s) 120, 339, 395
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 2 cut(s) 215, 223
CseI GACGC 2 cut(s) 192, 251
Csp6I GTAC 1 cut(s) 44
CviAII CATG 1 cut(s) 340
CviJI RGCY 7 cut(s) 73, 106, 216, 224, 264, 359, 420
CviKI_1 RGCY 7 cut(s) 73, 106, 216, 224, 264, 359, 420
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 6 cut(s) 360, 480, 495, 510, 525, 540
DpnI GATC 4 cut(s) 66, 305, 314, 329
DpnII GATC 4 cut(s) 64, 303, 312, 327
DrdI GACNNNNNNGTC 3 cut(s) 514, 529, 544
DseDI GACNNNNNNGTC 3 cut(s) 514, 529, 544
EaeI YGGCCR 1 cut(s) 104
EciI GGCGGA 3 cut(s) 70, 190, 256
Eco130I CCWWGG 1 cut(s) 321
Eco31I GGTCTC 3 cut(s) 146, 284, 395
EcoO109I RGGNCCY 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 219
EcoT14I CCWWGG 1 cut(s) 321
ErhI CCWWGG 1 cut(s) 321
FaeI CATG 1 cut(s) 343
FaiI YATR 1 cut(s) 341
FatI CATG 1 cut(s) 339
FauI CCCGC 3 cut(s) 45, 233, 430
FblI GTMKAC 2 cut(s) 138, 548
FokI GGATG 1 cut(s) 310
GlaI GCGC 3 cut(s) 119, 338, 394
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 3 cut(s) 106, 216, 224
HapII CCGG 3 cut(s) 258, 294, 462
HgaI GACGC 2 cut(s) 192, 251
HhaI GCGC 3 cut(s) 120, 339, 395
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 1 cut(s) 343
Hin6I GCGC 3 cut(s) 118, 337, 393
HinP1I GCGC 3 cut(s) 118, 337, 393
HincII GTYRAC 3 cut(s) 91, 139, 549
HindII GTYRAC 3 cut(s) 91, 139, 549
HinfI GANTC 6 cut(s) 32, 56, 500, 515, 530, 545
HpaII CCGG 3 cut(s) 258, 294, 462
HphI GGTGA 4 cut(s) 151, 476, 484, 499
Hpy166II GTNNAC 3 cut(s) 91, 139, 549
Hpy188I TCNGA 4 cut(s) 7, 69, 400, 481
Hpy188III TCNNGA 2 cut(s) 151, 180
Hpy8I GTNNAC 3 cut(s) 91, 139, 549
Hpy99I CGWCG 3 cut(s) 140, 186, 209
HpyAV CCTTC 1 cut(s) 256
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4V TGCA 1 cut(s) 310
HpyF10VI GCNNNNNNNGC 7 cut(s) 115, 209, 251, 365, 377, 380, 452
HpyF3I CTNAG 6 cut(s) 360, 480, 495, 510, 525, 540
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 1 cut(s) 343
HspAI GCGC 3 cut(s) 118, 337, 393
Kzo9I GATC 4 cut(s) 64, 303, 312, 327
LmnI GCTCC 1 cut(s) 269
Lsp1109I GCAGC 2 cut(s) 358, 367
LweI GCATC 2 cut(s) 108, 433
MaeIII GTNAC 2 cut(s) 57, 125
MalI GATC 4 cut(s) 66, 305, 314, 329
MboI GATC 4 cut(s) 64, 303, 312, 327
MboII GAAGA 5 cut(s) 313, 502, 517, 532, 547
MflI RGATCY 2 cut(s) 303, 312
MhlI GDGCHC 1 cut(s) 20
MlyI GAGTC 4 cut(s) 65, 524, 539, 554
MspA1I CMGCKG 2 cut(s) 371, 455
MspI CCGG 3 cut(s) 258, 294, 462
MspR9I CCNGG 3 cut(s) 221, 295, 462
MvaI CCWGG 1 cut(s) 221
MwoI GCNNNNNNNGC 7 cut(s) 115, 209, 251, 365, 377, 380, 452
NciI CCSGG 2 cut(s) 295, 462
NdeII GATC 4 cut(s) 64, 303, 312, 327
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 3 cut(s) 193, 217, 265
NmeAIII GCCGAG 1 cut(s) 433
NmuCI GTSAC 1 cut(s) 57
NspI RCATGY 1 cut(s) 343
PaeI GCATGC 1 cut(s) 343
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 396
PfeI GAWTC 2 cut(s) 32, 500
PleI GAGTC 4 cut(s) 64, 523, 538, 553
PpsI GAGTC 4 cut(s) 64, 523, 538, 553
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 3 cut(s) 193, 217, 265
PspPI GGNCC 2 cut(s) 215, 223
PstI CTGCAG 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 102
PsuI RGATCY 2 cut(s) 303, 312
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
SalI GTCGAC 2 cut(s) 137, 547
Sau3AI GATC 4 cut(s) 64, 303, 312, 327
Sau96I GGNCC 2 cut(s) 215, 223
SchI GAGTC 4 cut(s) 65, 524, 539, 554
ScrFI CCNGG 3 cut(s) 221, 295, 462
SduI GDGCHC 1 cut(s) 20
SetI ASST 7 cut(s) 30, 80, 197, 282, 474, 489, 554
SfaNI GCATC 2 cut(s) 108, 433
SfcI CTRYAG 2 cut(s) 94, 308
SgrAI CRCCGGYG 1 cut(s) 257
SphI GCATGC 1 cut(s) 343
StyD4I CCNGG 3 cut(s) 219, 293, 460
StyI CCWWGG 1 cut(s) 321
TaaI ACNGT 1 cut(s) 22
TaqI TCGA 9 cut(s) 30, 138, 150, 234, 345, 503, 518, 533, 548
TaqII GACCGA 1 cut(s) 128
TfiI GAWTC 2 cut(s) 32, 500
TscAI CASTG 1 cut(s) 292
TseFI GTSAC 1 cut(s) 57
TseI GCWGC 2 cut(s) 371, 380
Tsp45I GTSAC 1 cut(s) 57
TspDTI ATGAA 1 cut(s) 314
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 1 cut(s) 292
XceI RCATGY 1 cut(s) 343
XmiI GTMKAC 2 cut(s) 138, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.