Rh6AG480500

MFP1 attachment factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0010075
Physical Location & Seq
Reverse (-)
66165755 .. 66168104
2350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG480500.1

Sequence Viewer

Length: 492 bp
ATGTCAGACGCCGAGAGCACCCTGGAAGCCGTCGATTCCCAAGAGCCACCCAAGCAGCAGGAATCACAGGATCGGAAGCCCAGCTCCGGCGCCGCCTATAGCCTTTGGCCGCCGACTGACCGCACCCGCAACGCCGTCGTCGACCGTCTCATCGAGACCCTCAGCACTCCCTCCCCGCTCTCCAGACGATACGGCACCTTGTCCGCCGACGAGGCCAACGAGGAGTCCCGCCGAATCGAGCAAGACGCCTTCGCCGCCGCCGGGGGATCCGCCGCCACGGAGGTCAGCGGCGAGAACGGGATGAAGATCCTGCAGATCTACTCCAAGGAGATCAGCAAGCGCATGCTCGAGGTCGTCAAGGCTAAGAACGCCGCTGCTTCTGCTGCTGAAAACGGCGCGTCCGAGACCGCCGAGACTGGTAGCACCGCCGCCGCCGACGATGCCGCCGCCGTTCCCAGTGAGGATGTCAAGACTGAGACTGAGTCAACCTGA

Protein Analysis

163

Amino Acids

17.17

Weight (kDa)

4.48

Isoelectric Point (pI)

63.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WPP PF13943 34 - 128 2.9e-35 WPP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010075)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47200 AT5G27940 AT5G43070
fragaria_vesca FvH4_2g33670 FvH4_2g33670
malus_domestica MD08G1091600.v1.1 MD15G1075600.v1.1
prunus_persica Prupe.1G429000_v2.0.a1
pyrus_communis pycom08g07390 pycom15g07080
rosa_chinensis RchiOBHm_Chr6g0308881
rosa_laevigata RLG00000010608
rosa_multiflora Rmu_sc0004046.1_g000014
rosa_roxburghii Rroxscaffold_7G00159820
rosa_rugosa Rorug06G0368900 Rorug06G0369000
rosa_samantha Rh6AG480500 Rh6BG490000 Rh6CG495400 Rh6DG480800
rosa_wichuraiana Rw6G041880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 89, 194
AccBSI CCGCTC 1 cut(s) 178
AccI GTMKAC 1 cut(s) 141
AccII CGCG 1 cut(s) 398
AclWI GGATC 4 cut(s) 78, 261, 274, 301
AcoI YGGCCR 1 cut(s) 107
AcyI GRCGYC 3 cut(s) 9, 90, 246
AfiI CCNNNNNNNGG 2 cut(s) 86, 261
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 1 cut(s) 84
AluI AGCT 1 cut(s) 84
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 5 cut(s) 149, 152, 398, 407, 470
AlwI GGATC 4 cut(s) 78, 261, 274, 301
Ama87I CYCGRG 1 cut(s) 347
AoxI GGCC 2 cut(s) 107, 213
ApeKI GCWGC 3 cut(s) 55, 374, 383
AspLEI GCGC 3 cut(s) 92, 342, 398
AsuC2I CCSGG 1 cut(s) 262
AvaI CYCGRG 1 cut(s) 347
BamHI GGATCC 1 cut(s) 266
BanI GGYRCC 2 cut(s) 89, 194
Bbv12I GWGCWC 1 cut(s) 20
BbvCI CCTCAGC 1 cut(s) 161
BbvI GCAGC 3 cut(s) 67, 361, 370
BceAI ACGGC 5 cut(s) 14, 119, 208, 409, 434
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 1 cut(s) 23
BcnI CCSGG 1 cut(s) 262
BcoDI GTCTC 5 cut(s) 149, 152, 398, 407, 470
BfmI CTRYAG 2 cut(s) 97, 311
BfoI RGCGCY 1 cut(s) 93
BglI GCCNNNNNGGC 1 cut(s) 212
BglII AGATCT 1 cut(s) 315
Bme1390I CCNGG 2 cut(s) 23, 262
BmeT110I CYCGRG 1 cut(s) 347
BmiI GGNNCC 3 cut(s) 91, 196, 268
BmrFI CCNGG 2 cut(s) 23, 262
BmrI ACTGGG 1 cut(s) 450
BmsI GCATC 1 cut(s) 430
BmuI ACTGGG 1 cut(s) 450
BpmI CTGGAG 1 cut(s) 166
Bpu10I CCTNAGC 1 cut(s) 161
BpuMI CCSGG 1 cut(s) 262
BsaBI GATNNNNATC 1 cut(s) 305
BsaHI GRCGYC 3 cut(s) 9, 90, 246
BsaI GGTCTC 2 cut(s) 149, 398
BsaJI CCNNGG 4 cut(s) 21, 261, 276, 324
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 261
Bse1I ACTGG 2 cut(s) 421, 456
Bse8I GATNNNNATC 1 cut(s) 305
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 4 cut(s) 21, 261, 276, 324
BseGI GGATG 2 cut(s) 306, 469
BseJI GATNNNNATC 1 cut(s) 305
BseLI CCNNNNNNNGG 2 cut(s) 86, 261
BseMII CTCAG 3 cut(s) 175, 465, 471
BseNI ACTGG 2 cut(s) 421, 456
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 3 cut(s) 67, 361, 370
BseYI CCCAGC 1 cut(s) 80
Bsh1236I CGCG 1 cut(s) 398
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 2 cut(s) 109, 215
BshNI GGYRCC 2 cut(s) 89, 194
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 1 cut(s) 20
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 2 cut(s) 87, 261
BslFI GGGAC 1 cut(s) 211
BslI CCNNNNNNNGG 2 cut(s) 86, 261
BsmAI GTCTC 5 cut(s) 149, 152, 398, 407, 470
BsmBI CGTCTC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 211
BsnI GGCC 2 cut(s) 109, 215
Bso31I GGTCTC 2 cut(s) 149, 398
BsoBI CYCGRG 1 cut(s) 347
Bsp1286I GDGCHC 1 cut(s) 20
Bsp143I GATC 5 cut(s) 70, 266, 306, 315, 330
BspANI GGCC 2 cut(s) 109, 215
BspCNI CTCAG 3 cut(s) 174, 466, 472
BspFNI CGCG 1 cut(s) 398
BspLI GGNNCC 3 cut(s) 91, 196, 268
BspMAI CTGCAG 1 cut(s) 315
BspPI GGATC 4 cut(s) 78, 261, 274, 301
BspT107I GGYRCC 2 cut(s) 89, 194
BspTNI GGTCTC 2 cut(s) 149, 398
BsrBI CCGCTC 1 cut(s) 178
BsrI ACTGG 2 cut(s) 421, 456
BssECI CCNNGG 4 cut(s) 21, 261, 276, 324
BssMI GATC 5 cut(s) 70, 266, 306, 315, 330
BssNI GRCGYC 3 cut(s) 9, 90, 246
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 146
BstACI GRCGYC 3 cut(s) 9, 90, 246
BstC8I GCNNGC 2 cut(s) 338, 344
BstDEI CTNAG 4 cut(s) 161, 363, 474, 480
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 2 cut(s) 306, 469
BstFNI CGCG 1 cut(s) 398
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 3 cut(s) 92, 342, 398
BstKTI GATC 5 cut(s) 73, 269, 309, 318, 333
BstMAI GTCTC 5 cut(s) 149, 152, 398, 407, 470
BstMBI GATC 5 cut(s) 70, 266, 306, 315, 330
BstMCI CGRYCG 1 cut(s) 145
BstMWI GCNNNNNNNGC 7 cut(s) 52, 212, 254, 368, 380, 383, 440
BstNI CCWGG 1 cut(s) 23
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 21, 260
BstSFI CTRYAG 2 cut(s) 97, 311
BstUI CGCG 1 cut(s) 398
BstV1I GCAGC 3 cut(s) 67, 361, 370
BstX2I RGATCY 3 cut(s) 266, 306, 315
BstYI RGATCY 3 cut(s) 266, 306, 315
BsuRI GGCC 2 cut(s) 109, 215
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 2 cut(s) 306, 469
BtsIMutI CAGTG 1 cut(s) 463
Cac8I GCNNGC 2 cut(s) 338, 344
CfoI GCGC 3 cut(s) 92, 342, 398
CseI GACGC 3 cut(s) 17, 254, 387
CviAII CATG 1 cut(s) 343
CviJI RGCY 8 cut(s) 29, 46, 79, 84, 102, 109, 215, 362
CviKI_1 RGCY 8 cut(s) 29, 46, 79, 84, 102, 109, 215, 362
DdeI CTNAG 4 cut(s) 161, 363, 474, 480
DinI GGCGCC 1 cut(s) 91
DpnI GATC 5 cut(s) 72, 268, 308, 317, 332
DpnII GATC 5 cut(s) 70, 266, 306, 315, 330
EaeI YGGCCR 1 cut(s) 107
EciI GGCGGA 2 cut(s) 193, 259
Eco130I CCWWGG 1 cut(s) 324
Eco31I GGTCTC 2 cut(s) 149, 398
Eco88I CYCGRG 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 324
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 324
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 1 cut(s) 346
FaiI YATR 2 cut(s) 99, 344
FaqI GGGAC 1 cut(s) 211
FatI CATG 1 cut(s) 342
FauI CCCGC 3 cut(s) 134, 183, 236
FblI GTMKAC 1 cut(s) 141
FokI GGATG 2 cut(s) 313, 476
GlaI GCGC 3 cut(s) 91, 341, 397
GsaI CCCAGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 166
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 2 cut(s) 109, 215
HapII CCGG 2 cut(s) 87, 261
HgaI GACGC 3 cut(s) 17, 254, 387
HhaI GCGC 3 cut(s) 92, 342, 398
Hin1I GRCGYC 3 cut(s) 9, 90, 246
Hin1II CATG 1 cut(s) 346
Hin6I GCGC 3 cut(s) 90, 340, 396
HinP1I GCGC 3 cut(s) 90, 340, 396
HincII GTYRAC 2 cut(s) 142, 486
HindII GTYRAC 2 cut(s) 142, 486
HinfI GANTC 5 cut(s) 35, 62, 224, 234, 482
HpaII CCGG 2 cut(s) 87, 261
Hpy166II GTNNAC 2 cut(s) 142, 486
Hpy188I TCNGA 3 cut(s) 7, 75, 403
Hpy188III TCNNGA 3 cut(s) 154, 183, 469
Hpy8I GTNNAC 2 cut(s) 142, 486
Hpy99I CGWCG 5 cut(s) 35, 140, 143, 212, 440
HpyAV CCTTC 1 cut(s) 259
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4V TGCA 1 cut(s) 313
HpyF10VI GCNNNNNNNGC 7 cut(s) 52, 212, 254, 368, 380, 383, 440
HpyF3I CTNAG 4 cut(s) 161, 363, 474, 480
Hsp92I GRCGYC 3 cut(s) 9, 90, 246
Hsp92II CATG 1 cut(s) 346
HspAI GCGC 3 cut(s) 90, 340, 396
KasI GGCGCC 1 cut(s) 89
Kzo9I GATC 5 cut(s) 70, 266, 306, 315, 330
LmnI GCTCC 1 cut(s) 89
Lsp1109I GCAGC 3 cut(s) 67, 361, 370
LweI GCATC 1 cut(s) 430
MalI GATC 5 cut(s) 72, 268, 308, 317, 332
MbiI CCGCTC 1 cut(s) 178
MboI GATC 5 cut(s) 70, 266, 306, 315, 330
MboII GAAGA 1 cut(s) 316
MflI RGATCY 3 cut(s) 266, 306, 315
MhlI GDGCHC 1 cut(s) 20
Mly113I GGCGCC 1 cut(s) 90
MlyI GAGTC 2 cut(s) 233, 491
MnlI CCTC 7 cut(s) 170, 181, 205, 214, 274, 343, 454
MspA1I CMGCKG 2 cut(s) 288, 374
MspI CCGG 2 cut(s) 87, 261
MspR9I CCNGG 2 cut(s) 23, 262
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 1 cut(s) 398
MwoI GCNNNNNNNGC 7 cut(s) 52, 212, 254, 368, 380, 383, 440
NarI GGCGCC 1 cut(s) 90
NciI CCSGG 1 cut(s) 262
NdeII GATC 5 cut(s) 70, 266, 306, 315, 330
NlaIII CATG 1 cut(s) 346
NlaIV GGNNCC 3 cut(s) 91, 196, 268
NmeAIII GCCGAG 2 cut(s) 37, 436
NspI RCATGY 1 cut(s) 346
PaeI GCATGC 1 cut(s) 346
PaeR7I CTCGAG 1 cut(s) 347
PcsI WCGNNNNNNNCGW 3 cut(s) 138, 216, 399
PfeI GAWTC 3 cut(s) 35, 62, 234
PflFI GACNNNGTC 1 cut(s) 481
PleI GAGTC 2 cut(s) 232, 490
PluTI GGCGCC 1 cut(s) 93
PpsI GAGTC 2 cut(s) 232, 490
Psp6I CCWGG 1 cut(s) 21
PspFI CCCAGC 1 cut(s) 80
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 3 cut(s) 91, 196, 268
PspXI VCTCGAGB 1 cut(s) 347
PstI CTGCAG 1 cut(s) 315
PsuI RGATCY 3 cut(s) 266, 306, 315
PsyI GACNNNGTC 1 cut(s) 481
SalI GTCGAC 1 cut(s) 140
Sau3AI GATC 5 cut(s) 70, 266, 306, 315, 330
SchI GAGTC 2 cut(s) 233, 491
ScrFI CCNGG 2 cut(s) 23, 262
SduI GDGCHC 1 cut(s) 20
SetI ASST 5 cut(s) 86, 200, 285, 354, 491
SfaNI GCATC 1 cut(s) 430
SfcI CTRYAG 2 cut(s) 97, 311
SfoI GGCGCC 1 cut(s) 91
Sfr274I CTCGAG 1 cut(s) 347
SlaI CTCGAG 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
SphI GCATGC 1 cut(s) 346
SspDI GGCGCC 1 cut(s) 89
StyD4I CCNGG 2 cut(s) 21, 260
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 146
TaqI TCGA 5 cut(s) 33, 141, 153, 237, 348
TfiI GAWTC 3 cut(s) 35, 62, 234
TscAI CASTG 1 cut(s) 463
TseI GCWGC 3 cut(s) 55, 374, 383
TspDTI ATGAA 1 cut(s) 317
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 1 cut(s) 463
Tth111I GACNNNGTC 1 cut(s) 481
XceI RCATGY 1 cut(s) 346
XhoI CTCGAG 1 cut(s) 347
XmiI GTMKAC 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.