AT5G35320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012168
Physical Location & Seq
Forward (+)
13521746 .. 13523506
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35320.1

Sequence Viewer

Length: 678 bp
ATGGAGGAGAACGAGCGCTTGTCGATGAAGAGGAAAGATATTGACAGAGTAAACGATGACTTCTCTGATTTTTCCCTCTCTTCTCCCGCCAGGAAGATTCGTCGTCTGGATGTGGATTTGCCACCGATTATGGAGGAAGAAGAGATTGATTTACCGATGCAAGATACGGTCGCGGAAGAAATCGAGCTTGAGCCGGTTAACGATGAGAGAGCAATTGTTCTATTTAAGCCTCTTCATTATCATCAGCCTTCTTCTGGAAACGTATTTGTTGATCGACACTTGATTTCTGGGTTTAAGAATAGATTTCTTCGCGATGTATCGATAGCTGATGATAATCAGTATGAAGATGAGAGATTAAACAAGTGTCAAGCTGTTGTTTGCTGGAATCCGTCTCAGTCTACTTACTCACAGTCCATAGGAACCTTCCAACAACCGCGGACACTAGAGATTACTGAGTTGGATGAGACAGGTGAGGATGTGGTAATGGATGATGCATCCAATGAGATAGAAGAGGATACTGGAAGCACCAGTCTATCTTTCCCTCAGCAAGGACAGCAGCAAGAGCAAGAGCACACCTATGGTTTTGGCTTGCATCCGTGGCAGCAAGCGCAAAACTGTATGATTCCGCAGCTTCCTCAAGTCAGTACTACTCCCACTCCCATCACTTGGTTCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1668

Protein Analysis

225

Amino Acids

25.99

Weight (kDa)

4.37

Isoelectric Point (pI)

58.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35320
fragaria_vesca FvH4_4g06450 FvH4_4g06450
malus_domestica MD13G1236100.v1.1 MD16G1241100.v1.1
prunus_persica Prupe.1G067800_v2.0.a1
pyrus_communis pycom16g20180
rosa_chinensis RchiOBHm_Chr4g0398241
rosa_laevigata RLG00000009375
rosa_multiflora Rmu_sc0004094.1_g000005 Rmu_ssc0000112.1_g000002
rosa_roxburghii Rroxscaffold_5G00343220
rosa_rugosa Rorug04G0007100 Rorug04G0007100
rosa_samantha Rh4AG084500 Rh4BG081900 Rh4CG091900 Rh4DG077000
rosa_wichuraiana Rw4G007020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 666
AccI GTMKAC 1 cut(s) 398
AccII CGCG 3 cut(s) 173, 312, 436
AciI CCGC 5 cut(s) 87, 173, 434, 436, 626
AfaI GTAC 1 cut(s) 646
AfeI AGCGCT 1 cut(s) 17
AfiI CCNNNNNNNGG 3 cut(s) 254, 548, 666
AjnI CCWGG 1 cut(s) 89
AluBI AGCT 4 cut(s) 187, 326, 371, 631
AluI AGCT 4 cut(s) 187, 326, 371, 631
Alw21I GWGCWC 1 cut(s) 573
Alw26I GTCTC 2 cut(s) 396, 458
Aor51HI AGCGCT 1 cut(s) 17
ApeKI GCWGC 3 cut(s) 556, 601, 628
AspLEI GCGC 2 cut(s) 18, 610
AsuHPI GGTGA 1 cut(s) 482
Bbv12I GWGCWC 1 cut(s) 573
BbvCI CCTCAGC 1 cut(s) 543
BbvI GCAGC 3 cut(s) 568, 613, 640
BccI CCATC 1 cut(s) 668
BciT130I CCWGG 1 cut(s) 91
BciVI GTATCC 1 cut(s) 508
BcoDI GTCTC 2 cut(s) 396, 458
BfaI CTAG 1 cut(s) 443
BfoI RGCGCY 1 cut(s) 19
BfuI GTATCC 1 cut(s) 508
BisI GCNGC 3 cut(s) 557, 602, 629
BlsI GCNGC 3 cut(s) 558, 603, 630
BmcAI AGTACT 1 cut(s) 646
Bme1390I CCNGG 1 cut(s) 91
BmiI GGNNCC 2 cut(s) 421, 671
BmrFI CCNGG 1 cut(s) 91
BmsI GCATC 4 cut(s) 147, 481, 503, 601
Bpu10I CCTNAGC 1 cut(s) 543
BpuEI CTTGAG 2 cut(s) 209, 621
Bsa29I ATCGAT 1 cut(s) 320
BsaBI GATNNNNATC 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 434, 596
BsaXI ACNNNNNCTCC 2 cut(s) 640, 670
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 548, 666
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 2 cut(s) 523, 528
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 1 cut(s) 91
BseCI ATCGAT 1 cut(s) 320
BseDI CCNNGG 2 cut(s) 434, 596
BseGI GGATG 6 cut(s) 115, 466, 481, 493, 494, 592
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 3 cut(s) 254, 548, 666
BseMII CTCAG 3 cut(s) 407, 444, 557
BseNI ACTGG 2 cut(s) 523, 528
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 3 cut(s) 568, 613, 640
Bsh1236I CGCG 3 cut(s) 173, 312, 436
Bsh1285I CGRYCG 1 cut(s) 171
BshVI ATCGAT 1 cut(s) 320
BsiEI CGRYCG 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 573
BsiSI CCGG 1 cut(s) 194
BslI CCNNNNNNNGG 3 cut(s) 254, 548, 666
BsmAI GTCTC 2 cut(s) 396, 458
BsmBI CGTCTC 1 cut(s) 396
Bsp1286I GDGCHC 1 cut(s) 573
Bsp143I GATC 1 cut(s) 271
Bsp68I TCGCGA 1 cut(s) 312
BspACI CCGC 5 cut(s) 87, 173, 434, 436, 626
BspCNI CTCAG 3 cut(s) 406, 445, 556
BspDI ATCGAT 1 cut(s) 320
BspFNI CGCG 3 cut(s) 173, 312, 436
BspLI GGNNCC 2 cut(s) 421, 671
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 2 cut(s) 523, 528
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 2 cut(s) 434, 596
BssMI GATC 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 91
Bst4CI ACNGT 3 cut(s) 169, 411, 617
Bst6I CTCTTC 5 cut(s) 23, 85, 135, 237, 504
BstC8I GCNNGC 2 cut(s) 590, 606
BstDEI CTNAG 3 cut(s) 393, 453, 543
BstDSI CCRYGG 2 cut(s) 434, 596
BstENI CCTNNNNNAGG 1 cut(s) 546
BstF5I GGATG 6 cut(s) 115, 466, 481, 493, 494, 592
BstFNI CGCG 3 cut(s) 173, 312, 436
BstH2I RGCGCY 1 cut(s) 19
BstHHI GCGC 2 cut(s) 18, 610
BstKTI GATC 1 cut(s) 274
BstMAI GTCTC 2 cut(s) 396, 458
BstMBI GATC 1 cut(s) 271
BstMCI CGRYCG 1 cut(s) 171
BstMWI GCNNNNNNNGC 4 cut(s) 553, 562, 598, 607
BstNI CCWGG 1 cut(s) 91
BstSCI CCNGG 1 cut(s) 89
BstUI CGCG 3 cut(s) 173, 312, 436
BstV1I GCAGC 3 cut(s) 568, 613, 640
Bsu15I ATCGAT 1 cut(s) 320
BsuI GTATCC 1 cut(s) 508
BsuTUI ATCGAT 1 cut(s) 320
BtgI CCRYGG 2 cut(s) 434, 596
BtgZI GCGATG 1 cut(s) 327
BtsCI GGATG 6 cut(s) 115, 466, 481, 493, 494, 592
BtuMI TCGCGA 1 cut(s) 312
Cac8I GCNNGC 2 cut(s) 590, 606
CfoI GCGC 2 cut(s) 18, 610
Cfr10I RCCGGY 1 cut(s) 193
Cfr42I CCGCGG 1 cut(s) 437
ClaI ATCGAT 1 cut(s) 320
Csp6I GTAC 1 cut(s) 645
CviJI RGCY 8 cut(s) 187, 193, 229, 247, 326, 371, 588, 631
CviKI_1 RGCY 8 cut(s) 187, 193, 229, 247, 326, 371, 588, 631
CviQI GTAC 1 cut(s) 645
DdeI CTNAG 3 cut(s) 393, 453, 543
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
Eam1104I CTCTTC 5 cut(s) 23, 85, 135, 237, 504
EarI CTCTTC 5 cut(s) 23, 85, 135, 237, 504
Eco47III AGCGCT 1 cut(s) 17
EcoNI CCTNNNNNAGG 1 cut(s) 546
EcoRII CCWGG 1 cut(s) 89
EcoT22I ATGCAT 1 cut(s) 496
Esp3I CGTCTC 1 cut(s) 396
FaiI YATR 5 cut(s) 131, 342, 416, 579, 620
FauI CCCGC 1 cut(s) 94
FblI GTMKAC 1 cut(s) 398
Fnu4HI GCNGC 3 cut(s) 557, 602, 629
FokI GGATG 6 cut(s) 122, 473, 481, 488, 500, 579
Fsp4HI GCNGC 3 cut(s) 557, 602, 629
FspBI CTAG 1 cut(s) 443
GlaI GCGC 2 cut(s) 17, 609
GluI GCNGC 3 cut(s) 557, 602, 629
HaeII RGCGCY 1 cut(s) 19
HapII CCGG 1 cut(s) 194
HhaI GCGC 2 cut(s) 18, 610
Hin6I GCGC 2 cut(s) 16, 608
HinP1I GCGC 2 cut(s) 16, 608
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 3 cut(s) 97, 385, 622
HpaI GTTAAC 1 cut(s) 199
HpaII CCGG 1 cut(s) 194
HphI GGTGA 1 cut(s) 482
Hpy166II GTNNAC 3 cut(s) 52, 199, 399
Hpy188I TCNGA 2 cut(s) 67, 674
Hpy188III TCNNGA 3 cut(s) 107, 255, 311
Hpy8I GTNNAC 3 cut(s) 52, 199, 399
Hpy99I CGWCG 1 cut(s) 105
HpyAV CCTTC 2 cut(s) 258, 433
HpyCH4III ACNGT 3 cut(s) 169, 411, 617
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 3 cut(s) 160, 494, 592
HpyF10VI GCNNNNNNNGC 4 cut(s) 553, 562, 598, 607
HpyF3I CTNAG 3 cut(s) 393, 453, 543
HpySE526I ACGT 1 cut(s) 261
HspAI GCGC 2 cut(s) 16, 608
KspAI GTTAAC 1 cut(s) 199
KspI CCGCGG 1 cut(s) 437
Kzo9I GATC 1 cut(s) 271
Lsp1109I GCAGC 3 cut(s) 568, 613, 640
LweI GCATC 4 cut(s) 147, 481, 503, 601
MaeI CTAG 1 cut(s) 443
MaeII ACGT 1 cut(s) 261
MalI GATC 1 cut(s) 273
MboI GATC 1 cut(s) 271
MfeI CAATTG 1 cut(s) 213
MhlI GDGCHC 1 cut(s) 573
MluCI AATT 1 cut(s) 213
MmeI TCCRAC 2 cut(s) 438, 451
MnlI CCTC 8 cut(s) 24, 86, 127, 240, 466, 505, 552, 645
Mph1103I ATGCAT 1 cut(s) 496
MseI TTAA 4 cut(s) 198, 225, 294, 356
MslI CAYNNNNRTG 1 cut(s) 576
MspA1I CMGCKG 1 cut(s) 436
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 1 cut(s) 91
MunI CAATTG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 91
MvnI CGCG 3 cut(s) 173, 312, 436
MwoI GCNNNNNNNGC 4 cut(s) 553, 562, 598, 607
NdeII GATC 1 cut(s) 271
NlaIV GGNNCC 2 cut(s) 421, 671
NruI TCGCGA 1 cut(s) 312
NsiI ATGCAT 1 cut(s) 496
PfeI GAWTC 3 cut(s) 97, 385, 622
PflMI CCANNNNNTGG 1 cut(s) 666
PkrI GCNGC 3 cut(s) 558, 603, 630
Psp6I CCWGG 1 cut(s) 89
PspGI CCWGG 1 cut(s) 89
PspN4I GGNNCC 2 cut(s) 421, 671
RruI TCGCGA 1 cut(s) 312
RsaI GTAC 1 cut(s) 646
RsaNI GTAC 1 cut(s) 645
RseI CAYNNNNRTG 1 cut(s) 576
SacII CCGCGG 1 cut(s) 437
SaqAI TTAA 4 cut(s) 198, 225, 294, 356
SatI GCNGC 3 cut(s) 557, 602, 629
Sau3AI GATC 1 cut(s) 271
ScaI AGTACT 1 cut(s) 646
ScrFI CCNGG 1 cut(s) 91
SduI GDGCHC 1 cut(s) 573
SetI ASST 8 cut(s) 189, 264, 328, 373, 425, 472, 578, 633
SfaNI GCATC 4 cut(s) 147, 481, 503, 601
Sfr303I CCGCGG 1 cut(s) 437
SgrBI CCGCGG 1 cut(s) 437
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 2 cut(s) 188, 636
SmoI CTYRAG 2 cut(s) 188, 636
Sse9I AATT 1 cut(s) 213
SsiI CCGC 5 cut(s) 87, 173, 434, 436, 626
SspMI CTAG 1 cut(s) 443
StyD4I CCNGG 1 cut(s) 89
TaaI ACNGT 3 cut(s) 169, 411, 617
TaiI ACGT 1 cut(s) 264
TaqI TCGA 4 cut(s) 23, 183, 274, 320
TasI AATT 1 cut(s) 213
TatI WGTACW 1 cut(s) 644
TfiI GAWTC 3 cut(s) 97, 385, 622
Tru1I TTAA 4 cut(s) 198, 225, 294, 356
Tru9I TTAA 4 cut(s) 198, 225, 294, 356
TseI GCWGC 3 cut(s) 556, 601, 628
TspDTI ATGAA 3 cut(s) 41, 224, 357
TspGWI ACGGA 2 cut(s) 378, 585
Van91I CCANNNNNTGG 1 cut(s) 666
XagI CCTNNNNNAGG 1 cut(s) 546
XmiI GTMKAC 1 cut(s) 398
XspI CTAG 1 cut(s) 443
ZrmI AGTACT 1 cut(s) 646
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.