pycom16g20180

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012168
Physical Location & Seq
Forward (+)
17879106 .. 17880344
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g20180.3

Sequence Viewer

Length: 981 bp
ATGGGCACATGGATCACTTGTCAATTTTGTCTTCTGGAGAAATCTGGACAACTGCAGAAAGTAACAAACCTCTCTCTCTCTCTCTCTCTCTCCCCCCTCTTTTTACAGAGGGACAAGGACGATCCTTGTCCTTTACAGAGACTCTTTGAAGTTCCAGACTTTCAGGTTGTCTTTGCAAATCAAATAAAAAAAATCCAATCAAAACCTCAACAATCGCACAATTTAACAAAAACCCAAATCTCCGTTTCCTCAGAATCTGAAAACAGAGTTGCAGAAATCTCTCACATGGAGGACTACTTCGTCTCAGACGGAATGCTGATGAAAACGATGAAGAAGAAGAGGAAGGAGGTTGAGGAGCTTGACCAAGTCAACGAATACTTCTCCGACTTCTCTCTCTCTTCTCCCGCCAGAAAGATTCGCCGTCTGGATGCGCATTTGCCGCCCATCATGGAGGAGGAGGAGGCTGATTTTGCGGCGGCTCCGAATCAGGTGGAGTCGACTGCTCCAATTATTGAGGAATTGCCGTCAGAAAACAATGAGAAGGCCATTGTGCTCTTCAAGCCCATCATTTCCCCTCCTTTTTTCTCTTTACCTTCCGAATTGATCTGCGGCTTCAAGGACCAATTTCCGCGCTCCGGGCATTATGGTCCGAGACAATCAGCCGCGGGAGATGATGAGGCGGTGCAAAACAGCGAAAATCAATGTAAAGCTGTTGTTCCATGGGTTTATAGTAAGCTTCGGCCTAGGCCATCGATGGAGGTTTCGCAATCTGAGGCCCCGGAGGAGCTGATGGAGGCTGAGGAGATGGGAACAGCAACAATGGAGATTGAAGAAGAGAGCAGAGAAGGACAAGGGATGGCTAACGGGTGCGGCGGAGGAATGTGGACGAGTAATGAAGGGTTTCCTCAGTGGCAGCAGCAGCATTGTATGATTCCACAACCTCCACAAAACACCACCATGCCCATCACTTGGTTCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

327

Amino Acids

36.91

Weight (kDa)

4.82

Isoelectric Point (pI)

60.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35320
fragaria_vesca FvH4_4g06450 FvH4_4g06450
malus_domestica MD13G1236100.v1.1 MD16G1241100.v1.1
prunus_persica Prupe.1G067800_v2.0.a1
pyrus_communis pycom16g20180
rosa_chinensis RchiOBHm_Chr4g0398241
rosa_laevigata RLG00000009375
rosa_multiflora Rmu_sc0004094.1_g000005 Rmu_ssc0000112.1_g000002
rosa_roxburghii Rroxscaffold_5G00343220
rosa_rugosa Rorug04G0007100 Rorug04G0007100
rosa_samantha Rh4AG084500 Rh4BG081900 Rh4CG091900 Rh4DG077000
rosa_wichuraiana Rw4G007020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
Acc16I TGCGCA 1 cut(s) 432
AccB7I CCANNNNNTGG 1 cut(s) 969
AccI GTMKAC 1 cut(s) 497
AccII CGCG 2 cut(s) 631, 665
AclWI GGATC 2 cut(s) 20, 116
AfiI CCNNNNNNNGG 2 cut(s) 635, 969
AgsI TTSAA 4 cut(s) 149, 559, 616, 830
AluBI AGCT 4 cut(s) 358, 710, 736, 787
AluI AGCT 4 cut(s) 358, 710, 736, 787
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 3 cut(s) 133, 307, 646
AlwI GGATC 2 cut(s) 20, 116
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 4 cut(s) 543, 740, 746, 774
ApeKI GCWGC 3 cut(s) 913, 916, 919
Asp700I GAANNNNTTC 1 cut(s) 900
AspA2I CCTAGG 1 cut(s) 743
AspLEI GCGC 2 cut(s) 433, 633
AspS9I GGNCC 3 cut(s) 619, 647, 775
AsuC2I CCSGG 2 cut(s) 637, 779
AvaII GGWCC 2 cut(s) 619, 647
AvrII CCTAGG 1 cut(s) 743
BaeGI GKGCMC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 23
Bbv12I GWGCWC 1 cut(s) 555
BbvCI CCTCAGC 1 cut(s) 798
BbvI GCAGC 3 cut(s) 925, 928, 931
BccI CCATC 8 cut(s) 452, 572, 748, 757, 784, 799, 850, 971
BceAI ACGGC 2 cut(s) 405, 508
BcnI CCSGG 2 cut(s) 637, 779
BcoDI GTCTC 3 cut(s) 133, 307, 646
BfaI CTAG 1 cut(s) 744
BfmI CTRYAG 1 cut(s) 53
BisI GCNGC 9 cut(s) 440, 474, 477, 610, 663, 871, 914, 917, 920
BlnI CCTAGG 1 cut(s) 743
BlsI GCNGC 9 cut(s) 441, 475, 478, 611, 664, 872, 915, 918, 921
Bme1390I CCNGG 2 cut(s) 637, 779
Bme18I GGWCC 2 cut(s) 619, 647
BmgT120I GGNCC 3 cut(s) 619, 647, 775
BmiI GGNNCC 3 cut(s) 480, 777, 974
BmrFI CCNGG 2 cut(s) 637, 779
BmsI GCATC 1 cut(s) 418
BpiI GAAGAC 1 cut(s) 23
BpmI CTGGAG 1 cut(s) 56
Bpu10I CCTNAGC 1 cut(s) 798
BpuMI CCSGG 2 cut(s) 637, 779
Bsa29I ATCGAT 1 cut(s) 752
BsaJI CCNNGG 4 cut(s) 663, 719, 743, 777
Bsc4I CCNNNNNNNGG 2 cut(s) 635, 969
BseCI ATCGAT 1 cut(s) 752
BseDI CCNNGG 4 cut(s) 663, 719, 743, 777
BseGI GGATG 2 cut(s) 433, 861
BseLI CCNNNNNNNGG 2 cut(s) 635, 969
BseMII CTCAG 5 cut(s) 264, 318, 762, 789, 920
BseRI GAGGAG 6 cut(s) 368, 467, 470, 473, 797, 815
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 3 cut(s) 925, 928, 931
Bsh1236I CGCG 2 cut(s) 631, 665
BshFI GGCC 4 cut(s) 545, 742, 748, 776
BshVI ATCGAT 1 cut(s) 752
BsiHKAI GWGCWC 1 cut(s) 555
BsiSI CCGG 2 cut(s) 636, 779
BslFI GGGAC 1 cut(s) 125
BslI CCNNNNNNNGG 2 cut(s) 635, 969
BsmAI GTCTC 3 cut(s) 133, 307, 646
BsmBI CGTCTC 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 125
BsmI GAATGC 1 cut(s) 318
BsnI GGCC 4 cut(s) 545, 742, 748, 776
Bsp1286I GDGCHC 2 cut(s) 8, 555
Bsp143I GATC 3 cut(s) 12, 121, 603
Bsp19I CCATGG 1 cut(s) 719
BspANI GGCC 4 cut(s) 545, 742, 748, 776
BspCNI CTCAG 5 cut(s) 263, 317, 763, 790, 919
BspDI ATCGAT 1 cut(s) 752
BspFNI CGCG 2 cut(s) 631, 665
BspLI GGNNCC 3 cut(s) 480, 777, 974
BspMAI CTGCAG 1 cut(s) 57
BspPI GGATC 2 cut(s) 20, 116
BspQI GCTCTTC 1 cut(s) 560
BssECI CCNNGG 4 cut(s) 663, 719, 743, 777
BssMI GATC 3 cut(s) 12, 121, 603
BssT1I CCWWGG 2 cut(s) 719, 743
Bst6I CTCTTC 4 cut(s) 332, 403, 560, 828
BstDEI CTNAG 5 cut(s) 250, 304, 771, 798, 906
BstDSI CCRYGG 2 cut(s) 663, 719
BstF5I GGATG 2 cut(s) 433, 861
BstFNI CGCG 2 cut(s) 631, 665
BstHHI GCGC 2 cut(s) 433, 633
BstKTI GATC 3 cut(s) 15, 124, 606
BstMAI GTCTC 3 cut(s) 133, 307, 646
BstMBI GATC 3 cut(s) 12, 121, 603
BstMWI GCNNNNNNNGC 5 cut(s) 439, 470, 559, 637, 919
BstSCI CCNGG 2 cut(s) 635, 777
BstSFI CTRYAG 1 cut(s) 53
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 2 cut(s) 631, 665
BstV1I GCAGC 3 cut(s) 925, 928, 931
BstV2I GAAGAC 1 cut(s) 23
Bsu15I ATCGAT 1 cut(s) 752
BsuRI GGCC 4 cut(s) 545, 742, 748, 776
BsuTUI ATCGAT 1 cut(s) 752
BtgI CCRYGG 2 cut(s) 663, 719
BtsCI GGATG 2 cut(s) 433, 861
BtsIMutI CAGTG 1 cut(s) 914
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 2 cut(s) 433, 633
Cfr13I GGNCC 3 cut(s) 619, 647, 775
Cfr42I CCGCGG 1 cut(s) 666
ClaI ATCGAT 1 cut(s) 752
CviAII CATG 5 cut(s) 9, 286, 448, 720, 958
DdeI CTNAG 5 cut(s) 250, 304, 771, 798, 906
DpnI GATC 3 cut(s) 14, 123, 605
DpnII GATC 3 cut(s) 12, 121, 603
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
Eam1104I CTCTTC 4 cut(s) 332, 403, 560, 828
EarI CTCTTC 4 cut(s) 332, 403, 560, 828
EciI GGCGGA 1 cut(s) 888
Eco130I CCWWGG 2 cut(s) 719, 743
Eco47I GGWCC 2 cut(s) 619, 647
EcoO109I RGGNCCY 1 cut(s) 775
EcoT14I CCWWGG 2 cut(s) 719, 743
ErhI CCWWGG 2 cut(s) 719, 743
Esp3I CGTCTC 1 cut(s) 307
FaeI CATG 5 cut(s) 12, 289, 451, 723, 961
FaiI YATR 8 cut(s) 10, 287, 449, 645, 721, 729, 929, 959
FaqI GGGAC 1 cut(s) 125
FatI CATG 5 cut(s) 8, 285, 447, 719, 957
FauI CCCGC 2 cut(s) 412, 658
FblI GTMKAC 1 cut(s) 497
Fnu4HI GCNGC 9 cut(s) 440, 474, 477, 610, 663, 871, 914, 917, 920
FokI GGATG 2 cut(s) 440, 868
Fsp4HI GCNGC 9 cut(s) 440, 474, 477, 610, 663, 871, 914, 917, 920
FspAI RTGCGCAY 1 cut(s) 432
FspBI CTAG 1 cut(s) 744
FspI TGCGCA 1 cut(s) 432
GlaI GCGC 2 cut(s) 432, 632
GluI GCNGC 9 cut(s) 440, 474, 477, 610, 663, 871, 914, 917, 920
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 4 cut(s) 545, 742, 748, 776
HapII CCGG 2 cut(s) 636, 779
HhaI GCGC 2 cut(s) 433, 633
Hin1II CATG 5 cut(s) 12, 289, 451, 723, 961
Hin6I GCGC 2 cut(s) 431, 631
HinP1I GCGC 2 cut(s) 431, 631
HincII GTYRAC 2 cut(s) 370, 498
HindII GTYRAC 2 cut(s) 370, 498
HindIII AAGCTT 1 cut(s) 734
HinfI GANTC 6 cut(s) 141, 254, 415, 484, 494, 931
HpaII CCGG 2 cut(s) 636, 779
Hpy166II GTNNAC 3 cut(s) 370, 498, 885
Hpy188III TCNNGA 4 cut(s) 35, 45, 155, 425
Hpy8I GTNNAC 3 cut(s) 370, 498, 885
HpyAV CCTTC 5 cut(s) 337, 535, 603, 839, 890
HpyCH4V TGCA 4 cut(s) 55, 176, 272, 685
HpyF10VI GCNNNNNNNGC 5 cut(s) 439, 470, 559, 637, 919
HpyF3I CTNAG 5 cut(s) 250, 304, 771, 798, 906
Hsp92II CATG 5 cut(s) 12, 289, 451, 723, 961
HspAI GCGC 2 cut(s) 431, 631
KspI CCGCGG 1 cut(s) 666
Kzo9I GATC 3 cut(s) 12, 121, 603
LguI GCTCTTC 1 cut(s) 560
LmnI GCTCC 5 cut(s) 355, 484, 508, 638, 784
LpnPI CCDG 9 cut(s) 20, 30, 149, 168, 410, 421, 473, 649, 792
Lsp1109I GCAGC 3 cut(s) 925, 928, 931
LweI GCATC 1 cut(s) 418
MaeI CTAG 1 cut(s) 744
MaeIII GTNAC 1 cut(s) 61
MalI GATC 3 cut(s) 14, 123, 605
MboI GATC 3 cut(s) 12, 121, 603
MboII GAAGA 8 cut(s) 23, 343, 346, 349, 390, 547, 842, 845
MhlI GDGCHC 2 cut(s) 8, 555
MluCI AATT 6 cut(s) 23, 220, 507, 518, 599, 623
MlyI GAGTC 2 cut(s) 135, 503
MmeI TCCRAC 1 cut(s) 408
MroXI GAANNNNTTC 1 cut(s) 900
MseI TTAA 1 cut(s) 224
MslI CAYNNNNRTG 1 cut(s) 956
MspA1I CMGCKG 1 cut(s) 665
MspI CCGG 2 cut(s) 636, 779
MspR9I CCNGG 2 cut(s) 637, 779
Mva1269I GAATGC 1 cut(s) 318
MvnI CGCG 2 cut(s) 631, 665
MwoI GCNNNNNNNGC 5 cut(s) 439, 470, 559, 637, 919
NciI CCSGG 2 cut(s) 637, 779
NcoI CCATGG 1 cut(s) 719
NdeII GATC 3 cut(s) 12, 121, 603
NlaIII CATG 5 cut(s) 12, 289, 451, 723, 961
NlaIV GGNNCC 3 cut(s) 480, 777, 974
NsbI TGCGCA 1 cut(s) 432
PciSI GCTCTTC 1 cut(s) 560
PctI GAATGC 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 900
PfeI GAWTC 4 cut(s) 254, 415, 484, 931
PflFI GACNNNGTC 1 cut(s) 365
PflMI CCANNNNNTGG 1 cut(s) 969
PkrI GCNGC 9 cut(s) 441, 475, 478, 611, 664, 872, 915, 918, 921
PleI GAGTC 2 cut(s) 135, 502
PpsI GAGTC 2 cut(s) 135, 502
PspN4I GGNNCC 3 cut(s) 480, 777, 974
PspPI GGNCC 3 cut(s) 619, 647, 775
PstI CTGCAG 1 cut(s) 57
PstNI CAGNNNCTG 1 cut(s) 257
PsyI GACNNNGTC 1 cut(s) 365
RseI CAYNNNNRTG 1 cut(s) 956
SacII CCGCGG 1 cut(s) 666
SalI GTCGAC 1 cut(s) 496
SapI GCTCTTC 1 cut(s) 560
SaqAI TTAA 1 cut(s) 224
SatI GCNGC 9 cut(s) 440, 474, 477, 610, 663, 871, 914, 917, 920
Sau3AI GATC 3 cut(s) 12, 121, 603
Sau96I GGNCC 3 cut(s) 619, 647, 775
SchI GAGTC 2 cut(s) 135, 503
ScrFI CCNGG 2 cut(s) 637, 779
SduI GDGCHC 2 cut(s) 8, 555
SfaNI GCATC 1 cut(s) 418
SfcI CTRYAG 1 cut(s) 53
Sfr303I CCGCGG 1 cut(s) 666
SgrBI CCGCGG 1 cut(s) 666
SinI GGWCC 2 cut(s) 619, 647
SmiMI CAYNNNNRTG 1 cut(s) 956
Sse9I AATT 6 cut(s) 23, 220, 507, 518, 599, 623
SspMI CTAG 1 cut(s) 744
StyD4I CCNGG 2 cut(s) 635, 777
StyI CCWWGG 2 cut(s) 719, 743
TaqI TCGA 2 cut(s) 497, 752
TasI AATT 6 cut(s) 23, 220, 507, 518, 599, 623
TauI GCSGC 6 cut(s) 442, 476, 479, 612, 665, 873
TfiI GAWTC 4 cut(s) 254, 415, 484, 931
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TscAI CASTG 1 cut(s) 914
TseI GCWGC 3 cut(s) 913, 916, 919
TspDTI ATGAA 3 cut(s) 335, 344, 909
TspGWI ACGGA 2 cut(s) 232, 324
TspRI CASTG 1 cut(s) 914
Tth111I GACNNNGTC 1 cut(s) 365
Van91I CCANNNNNTGG 1 cut(s) 969
VpaK11BI GGWCC 2 cut(s) 619, 647
XmaJI CCTAGG 1 cut(s) 743
XmiI GTMKAC 1 cut(s) 497
XmnI GAANNNNTTC 1 cut(s) 900
XspI CTAG 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.