MD13G1236100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012168
Physical Location & Seq
Forward (+)
23853524 .. 23854945
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1236100.v1.1.491

Sequence Viewer

Length: 699 bp
ATGGAGGACTACTTTGTCTCAGACCGAATGCTGATGAAAACGATGAAGAAAAAGAGGAAGGAGGTGGAGGAGCTTGACCAAGTCAACGAAGACTTCTCCGACTTCTCTCTTTCTTCTCCCGCCAGAAAGATTCGCCGCCTGGATGCTCATTTGCCGCCCATCATGGAGGAGGAGGAGGCGGAGTTTGCGGTGGCTCCGAATCAGGTGAAATTAGATGCTCCAGTTATCGAGGAGTTGCCGTCAGAAAACCATGAGAAGGCCATTGTGCTCTTCAAGCCTGTCAGTTGCCCTCCCGTCTTCTCTCTTCGTTCCGATTTGATTTCCGGCTTCAAGGACCAATTTCTGTGCTCCAGCCATTATGATCTCAGACAATCAGCAGAGGAAGATGACGAGACGGTGCAAAACAAGGCGAATCGATGTAAAGCTGTTGTTCCATGGGTTCCAAGCCAGCTTCCGCCTGTGCCATCAATGGAGGTTTCGCAGCAATCTGAGGCCCCGGGAGAGCTGATGGAGGCTGAAGAGATGGGAACAGCAACAATGGAGATTGAAGAGGAGAGCACAGAAGGACAAGGCATGGCTAACGTGTATGGCGGGGGTATGTGGACGAGTAATGAAGGGTTTCCTGAGTGGCAGCAGCAGCATTGTATGATCCCACAGCCTCCACAAAACACCACCACACCTATCACTTGGTTCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

233

Amino Acids

26.36

Weight (kDa)

4.51

Isoelectric Point (pI)

73.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35320
fragaria_vesca FvH4_4g06450 FvH4_4g06450
malus_domestica MD13G1236100.v1.1 MD16G1241100.v1.1
prunus_persica Prupe.1G067800_v2.0.a1
pyrus_communis pycom16g20180
rosa_chinensis RchiOBHm_Chr4g0398241
rosa_laevigata RLG00000009375
rosa_multiflora Rmu_sc0004094.1_g000005 Rmu_ssc0000112.1_g000002
rosa_roxburghii Rroxscaffold_5G00343220
rosa_rugosa Rorug04G0007100 Rorug04G0007100
rosa_samantha Rh4AG084500 Rh4BG081900 Rh4CG091900 Rh4DG077000
rosa_wichuraiana Rw4G007020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 14
AciI CCGC 7 cut(s) 120, 136, 155, 179, 188, 455, 591
AclWI GGATC 1 cut(s) 643
AcuI CTGAAG 1 cut(s) 537
AfiI CCNNNNNNNGG 1 cut(s) 256
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 3 cut(s) 274, 331, 548
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 4 cut(s) 73, 425, 451, 505
AluI AGCT 4 cut(s) 73, 425, 451, 505
Alw21I GWGCWC 3 cut(s) 270, 350, 560
Alw26I GTCTC 2 cut(s) 22, 386
AlwI GGATC 1 cut(s) 643
Ama87I CYCGRG 1 cut(s) 496
AoxI GGCC 2 cut(s) 258, 492
ApeKI GCWGC 4 cut(s) 481, 631, 634, 637
Asp700I GAANNNNTTC 1 cut(s) 618
AspS9I GGNCC 2 cut(s) 334, 493
AsuC2I CCSGG 2 cut(s) 497, 498
AsuHPI GGTGA 1 cut(s) 217
AvaI CYCGRG 1 cut(s) 496
AvaII GGWCC 1 cut(s) 334
BbsI GAAGAC 2 cut(s) 96, 289
Bbv12I GWGCWC 3 cut(s) 270, 350, 560
BbvI GCAGC 4 cut(s) 493, 643, 646, 649
BccI CCATC 4 cut(s) 167, 472, 502, 517
BceAI ACGGC 1 cut(s) 223
BciT130I CCWGG 1 cut(s) 140
BcnI CCSGG 2 cut(s) 497, 498
BcoDI GTCTC 2 cut(s) 22, 386
BisI GCNGC 6 cut(s) 136, 155, 482, 632, 635, 638
BlsI GCNGC 6 cut(s) 137, 156, 483, 633, 636, 639
Bme1390I CCNGG 3 cut(s) 140, 497, 498
Bme18I GGWCC 1 cut(s) 334
BmeT110I CYCGRG 1 cut(s) 496
BmgT120I GGNCC 2 cut(s) 334, 493
BmiI GGNNCC 4 cut(s) 195, 441, 495, 692
BmrFI CCNGG 3 cut(s) 140, 497, 498
BmsI GCATC 2 cut(s) 133, 205
BpiI GAAGAC 2 cut(s) 96, 289
BpmI CTGGAG 2 cut(s) 204, 334
BpuMI CCSGG 2 cut(s) 497, 498
Bsa29I ATCGAT 1 cut(s) 415
BsaJI CCNNGG 3 cut(s) 434, 495, 496
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 224, 254
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse1I ACTGG 1 cut(s) 221
BseBI CCWGG 1 cut(s) 140
BseCI ATCGAT 1 cut(s) 415
BseDI CCNNGG 3 cut(s) 434, 495, 496
BseGI GGATG 1 cut(s) 148
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMII CTCAG 4 cut(s) 33, 379, 480, 615
BseNI ACTGG 1 cut(s) 221
BseRI GAGGAG 6 cut(s) 83, 182, 185, 188, 245, 566
BseXI GCAGC 4 cut(s) 493, 643, 646, 649
BshFI GGCC 2 cut(s) 260, 494
BshVI ATCGAT 1 cut(s) 415
BsiHKAI GWGCWC 3 cut(s) 270, 350, 560
BsiHKCI CYCGRG 1 cut(s) 496
BsiSI CCGG 2 cut(s) 324, 497
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 2 cut(s) 22, 386
BsmBI CGTCTC 1 cut(s) 386
BsmI GAATGC 1 cut(s) 33
BsnI GGCC 2 cut(s) 260, 494
BsoBI CYCGRG 1 cut(s) 496
Bsp1286I GDGCHC 3 cut(s) 270, 350, 560
Bsp143I GATC 2 cut(s) 361, 648
Bsp19I CCATGG 1 cut(s) 434
BspACI CCGC 7 cut(s) 120, 136, 155, 179, 188, 455, 591
BspANI GGCC 2 cut(s) 260, 494
BspCNI CTCAG 4 cut(s) 32, 378, 481, 616
BspDI ATCGAT 1 cut(s) 415
BspLI GGNNCC 4 cut(s) 195, 441, 495, 692
BspPI GGATC 1 cut(s) 643
BspQI GCTCTTC 1 cut(s) 275
BsrI ACTGG 1 cut(s) 221
BssECI CCNNGG 3 cut(s) 434, 495, 496
BssMI GATC 2 cut(s) 361, 648
BssT1I CCWWGG 1 cut(s) 434
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 397
Bst6I CTCTTC 4 cut(s) 275, 309, 513, 543
BstC8I GCNNGC 1 cut(s) 449
BstDEI CTNAG 4 cut(s) 19, 365, 489, 624
BstDSI CCRYGG 1 cut(s) 434
BstF5I GGATG 1 cut(s) 148
BstKTI GATC 2 cut(s) 364, 651
BstMAI GTCTC 2 cut(s) 22, 386
BstMBI GATC 2 cut(s) 361, 648
BstMWI GCNNNNNNNGC 3 cut(s) 185, 274, 637
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 3 cut(s) 138, 495, 496
BstV1I GCAGC 4 cut(s) 493, 643, 646, 649
BstV2I GAAGAC 2 cut(s) 96, 289
Bsu15I ATCGAT 1 cut(s) 415
BsuRI GGCC 2 cut(s) 260, 494
BsuTUI ATCGAT 1 cut(s) 415
BtgI CCRYGG 1 cut(s) 434
BtsCI GGATG 1 cut(s) 148
Cac8I GCNNGC 1 cut(s) 449
Cfr13I GGNCC 2 cut(s) 334, 493
Cfr9I CCCGGG 1 cut(s) 496
ClaI ATCGAT 1 cut(s) 415
CviAII CATG 4 cut(s) 163, 251, 435, 574
DdeI CTNAG 4 cut(s) 19, 365, 489, 624
DpnI GATC 2 cut(s) 363, 650
DpnII GATC 2 cut(s) 361, 648
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
Eam1104I CTCTTC 4 cut(s) 275, 309, 513, 543
EarI CTCTTC 4 cut(s) 275, 309, 513, 543
EciI GGCGGA 2 cut(s) 194, 444
Eco130I CCWWGG 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 537
Eco88I CYCGRG 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 434
ErhI CCWWGG 1 cut(s) 434
Esp3I CGTCTC 1 cut(s) 386
FaeI CATG 4 cut(s) 166, 254, 438, 577
FaiI YATR 8 cut(s) 164, 252, 360, 436, 575, 588, 599, 647
FatI CATG 4 cut(s) 162, 250, 434, 573
FauI CCCGC 2 cut(s) 127, 584
Fnu4HI GCNGC 6 cut(s) 136, 155, 482, 632, 635, 638
FokI GGATG 1 cut(s) 155
Fsp4HI GCNGC 6 cut(s) 136, 155, 482, 632, 635, 638
GluI GCNGC 6 cut(s) 136, 155, 482, 632, 635, 638
GsuI CTGGAG 2 cut(s) 204, 334
HaeIII GGCC 2 cut(s) 260, 494
HapII CCGG 2 cut(s) 324, 497
Hin1II CATG 4 cut(s) 166, 254, 438, 577
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 3 cut(s) 130, 199, 412
HpaII CCGG 2 cut(s) 324, 497
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 2 cut(s) 85, 603
Hpy188I TCNGA 8 cut(s) 22, 100, 198, 244, 313, 368, 490, 695
Hpy188III TCNNGA 1 cut(s) 623
Hpy8I GTNNAC 2 cut(s) 85, 603
HpyAV CCTTC 4 cut(s) 52, 250, 557, 608
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 1 cut(s) 582
HpyCH4V TGCA 1 cut(s) 400
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 274, 637
HpyF3I CTNAG 4 cut(s) 19, 365, 489, 624
HpySE526I ACGT 1 cut(s) 582
Hsp92II CATG 4 cut(s) 166, 254, 438, 577
Kzo9I GATC 2 cut(s) 361, 648
LguI GCTCTTC 1 cut(s) 275
LmnI GCTCC 4 cut(s) 70, 199, 223, 353
Lsp1109I GCAGC 4 cut(s) 493, 643, 646, 649
LweI GCATC 2 cut(s) 133, 205
MaeII ACGT 1 cut(s) 582
MalI GATC 2 cut(s) 363, 650
MboI GATC 2 cut(s) 361, 648
MboII GAAGA 9 cut(s) 58, 101, 105, 262, 289, 296, 395, 530, 560
MhlI GDGCHC 3 cut(s) 270, 350, 560
MluCI AATT 2 cut(s) 209, 338
MmeI TCCRAC 1 cut(s) 123
MroXI GAANNNNTTC 1 cut(s) 618
MspI CCGG 2 cut(s) 324, 497
MspR9I CCNGG 3 cut(s) 140, 497, 498
Mva1269I GAATGC 1 cut(s) 33
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 3 cut(s) 185, 274, 637
NciI CCSGG 2 cut(s) 497, 498
NcoI CCATGG 1 cut(s) 434
NdeII GATC 2 cut(s) 361, 648
NlaIII CATG 4 cut(s) 166, 254, 438, 577
NlaIV GGNNCC 4 cut(s) 195, 441, 495, 692
PciSI GCTCTTC 1 cut(s) 275
PctI GAATGC 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 618
PfeI GAWTC 3 cut(s) 130, 199, 412
PflFI GACNNNGTC 1 cut(s) 80
PkrI GCNGC 6 cut(s) 137, 156, 483, 633, 636, 639
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 4 cut(s) 195, 441, 495, 692
PspPI GGNCC 2 cut(s) 334, 493
PsyI GACNNNGTC 1 cut(s) 80
SapI GCTCTTC 1 cut(s) 275
SatI GCNGC 6 cut(s) 136, 155, 482, 632, 635, 638
Sau3AI GATC 2 cut(s) 361, 648
Sau96I GGNCC 2 cut(s) 334, 493
ScrFI CCNGG 3 cut(s) 140, 497, 498
SduI GDGCHC 3 cut(s) 270, 350, 560
SetI ASST 9 cut(s) 66, 75, 207, 427, 453, 477, 507, 585, 682
SfaNI GCATC 2 cut(s) 133, 205
SinI GGWCC 1 cut(s) 334
SmaI CCCGGG 1 cut(s) 498
Sse9I AATT 2 cut(s) 209, 338
SsiI CCGC 7 cut(s) 120, 136, 155, 179, 188, 455, 591
StyD4I CCNGG 3 cut(s) 138, 495, 496
StyI CCWWGG 1 cut(s) 434
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 1 cut(s) 585
TaqI TCGA 2 cut(s) 228, 415
TaqII GACCGA 1 cut(s) 39
TasI AATT 2 cut(s) 209, 338
TauI GCSGC 2 cut(s) 138, 157
TfiI GAWTC 3 cut(s) 130, 199, 412
TseI GCWGC 4 cut(s) 481, 631, 634, 637
TspDTI ATGAA 3 cut(s) 50, 59, 627
TspMI CCCGGG 1 cut(s) 496
Tth111I GACNNNGTC 1 cut(s) 80
VpaK11BI GGWCC 1 cut(s) 334
XmaI CCCGGG 1 cut(s) 496
XmnI GAANNNNTTC 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.