AT5G37180

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004012
Physical Location & Seq
Forward (+)
14718141 .. 14723139
4999 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G37180.2

Sequence Viewer

Length: 2514 bp
ATGGAAATGACATCTGGATCGTTAGGCAATGGGATCCCAGAAGCGATGGGACAAAACCGCGGGAATATTAAACGTTGCCTCGAGAAGTATATTGAGAATGGCAGAAGGGTGATGAAATTGAACGAGTTGATGGATGAAATGGAAATTGTTATCAATGATGTAACACAACGAAGGCGAGTCATGGAAGGCGATCTTGGCAAGATCTTGTGCTTCACTCAGGAGGCAGTGGTAATTCCTCCGAACGTAGCGTTCGCAGTAAGAGGAACCCCGGGAAATTGGCAATACGTGAAAGTTAACTCTTCCAATCTCTCGGTAGAAGCTCTATCGAGCACGCAATATCTCAAACTTAAAGAGTTTCTCTTCGACGAGAATTGGGCAAACGATGAAAATGCATTGGAGGTTGATTTTGGAGCGCTGGATTTCACTCTTCCTTGGCTTTCGTTGTCATCTTCAATAGGAAACGGCCTGAGTTTTGTGTCCTCGAAGCTCGGAGGGCGTTTAAACGATAACCCGCAATCTCTCGTGGACTACTTGTTGTCACTTGAGCATCAAGGAGAGAAGCTTATGATGAATGAGACTCTTAACACGGCTAGAAAGCTTGAGATGAGTTTGATTTTGGCAGATGTTTTCCTATCGGAGCTGCCTAAGGACACACCTTTCCAAGCCTTTGAACTCAGGTTTAAGGAATGTGGTTTTGAGAAAGGATGGGGAGAAAGCGCAGGGAGAGTAAAGGAGACAATGAGAATCTTGTCGGAGATTCTTCAAGCCCCTGATCCTCAAAACATTGATCGATTCTTCGCAAGGGTTCCTAGAATCTTTAACGTTGTCATCTTCTCGGTTCACGGCTACTTTGGTCAAACCGATGTTCTAGGTTTGCCTGATACTGGCGGTCAAGTCGTTTACATTCTTGACCAAGTTAAAGCCCTTGAAGATGAATTGCTTCAAAGAATTAACTCTCAAGGCCTTAATTTCAAACCTCAGATTCTTGTTGTAACACGATTAATCCCAGACGCTAAAAAAACTAAATGCAACCAAGAGTTGGAACCTATTTTCGGAACGAAATATTCGAATATTCTTCGAATTCCATTTGTAACAGAAAATGGGATTTTACGTCGTTGGGTTTCCCGCTTTGATATCTACCCATACCTAGAAAGATTCACTAAGGATGCAACAACAAAGATATTGGATATTTTGGAAGGAAAACCTGACCTAATAATCGGGAACTATACGGATGGAAACTTGGTGGCATCACTCATGGCTAATAAGCTTGGAATTACTCAGGCAACTATTGCACATGCCTTGGAGAAGACTAAATATGAAGATTCAGATATCAAGTGGAAAGAGTTTGATCCTAAATATCATTTCTCATCTCAATTTACGGCAGACTTAATCTCTATGAACTCTGCCGATTTCATCATAGCTAGCACTTATCAAGAAATTGCTGGAAGCAAAGAGAGGGCTGGACAATACGAGAGCCACATGAGCTTCACAGTCCCGGGGCTATACAGAGTTGTCTCCGGCATTAATGTCTTCGATCCGAGGTTCAACATCGCAGCTCCTGGCGCGGATGACTCTATCTATTTCCCTTTTACCGCACAAGACCGGAGATTCACCAAGTTCTACACTTCCATTGATGAACTCTTGTATAGCCAAAGTGAGAATGACGAACACATCGGTTATTTAGTGGACAAGAAGAAACCGATCATTTTCTCAATGGCAAGGCTTGATGTTGTCAAAAACCTAACCGGATTAACTGAGTGGTATGCCAAGAACAAGAGGCTACGAGACTTGGTGAACCTCGTGATCGTAGGAGGATTCTTCGACGCTTCTAAGTCTAAAGACAGGGAAGAGATCTCAGAGATCAAGAAGATGCATTCTCTGATTGAGAAATACCAACTCAAGGGTCAATTTAGATGGATCACAGCTCAAACAGATCGAACAAGAAACGGTGAACTTTACCGATCTATCGCGGATACAAGAGGTGCTTTTGTGCAGCCTGCACATTATGAAGCCTTTGGTCTTACTGTCATTGAAGCCATGAGCTGTGGTTTGGTCACATTCGCCACGAACCAAGGCGGTCCTGCTGAGATTATTGTGGATGGTGTCTCTGGTTTCCACATTGACCCGAGCAATGGAGAAGAATCAAGTGATAAGATTGCGGATTTCTTTGAGAAATCCGGTATGGATCCTGATTATTGGAATATGTTTTCGAATGAAGGGCTACAACGCATAAACGAATGCTATACATGGAAGATATATGCAAACAAAGTGATAAACATGGGGAGTACATATAGCTACTGGAGACATTTGAACAAAGATCAGAAACTGGCCAAACAAAGATATATCCATTCCTTCTACAATCTTCAATACCGAAATTTGGTGAAAACTATACCCATATTGAGTGATATTCCTGAGCCTCCTCCTCTTCCTCCAAAGCCTTTGGTGAAACCATCAGCATCAAAAGGCTCGAAGCGAACACAGCCGCGATTGAGTTTCAGGTTGTTTGGTGCTTAA

Protein Analysis

837

Amino Acids

95.03

Weight (kDa)

6.13

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_EPBD PF24862 1 - 50 2.5e-13 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 73 - 361 7.5e-167 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 372 - 538 2e-31 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 379 - 534 1.8e-12 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1039
AccII CGCG 4 cut(s) 60, 1565, 1970, 2485
AclI AACGTT 2 cut(s) 73, 822
AclWI GGATC 9 cut(s) 25, 28, 41, 767, 1343, 1529, 1925, 2180, 2193
AcoI YGGCCR 1 cut(s) 2328
AcsI RAATTY 2 cut(s) 1080, 2374
AfaI GTAC 1 cut(s) 2287
AfeI AGCGCT 1 cut(s) 414
AfiI CCNNNNNNNGG 6 cut(s) 884, 1039, 1052, 1900, 2132, 2377
AjnI CCWGG 1 cut(s) 1558
AjuI GAANNNNNNNTTGG 2 cut(s) 177, 209
Alw21I GWGCWC 1 cut(s) 332
Alw26I GTCTC 6 cut(s) 569, 728, 1519, 1779, 2110, 2296
AlwI GGATC 9 cut(s) 25, 28, 41, 767, 1343, 1529, 1925, 2180, 2193
AlwNI CAGNNNCTG 2 cut(s) 1559, 2326
Ama87I CYCGRG 4 cut(s) 80, 268, 1495, 2125
Aor51HI AGCGCT 1 cut(s) 414
AoxI GGCC 3 cut(s) 463, 961, 2328
ApeKI GCWGC 3 cut(s) 640, 1553, 1993
ApoI RAATTY 2 cut(s) 1080, 2374
AseI ATTAAT 2 cut(s) 1001, 1524
Asp700I GAANNNNTTC 1 cut(s) 939
AspLEI GCGC 3 cut(s) 415, 719, 1565
AspS9I GGNCC 1 cut(s) 2078
AsuC2I CCSGG 4 cut(s) 269, 270, 1496, 1497
AsuHPI GGTGA 6 cut(s) 121, 1603, 1804, 1961, 2392, 2455
AsuII TTCGAA 3 cut(s) 1067, 1078, 2210
AsuNHI GCTAGC 1 cut(s) 1421
AvaI CYCGRG 4 cut(s) 80, 268, 1495, 2125
AvaII GGWCC 1 cut(s) 2078
AxyI CCTNAGG 1 cut(s) 645
BalI TGGCCA 1 cut(s) 2330
BamHI GGATCC 2 cut(s) 33, 2185
BauI CACGAG 2 cut(s) 521, 1799
BbsI GAAGAC 2 cut(s) 1313, 1522
Bbv12I GWGCWC 1 cut(s) 332
BbvI GCAGC 3 cut(s) 627, 1565, 2005
BccI CCATC 7 cut(s) 40, 124, 699, 1226, 1908, 2093, 2458
BceAI ACGGC 4 cut(s) 478, 603, 859, 1395
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BciT130I CCWGG 1 cut(s) 1560
BciVI GTATCC 1 cut(s) 1966
BcnI CCSGG 4 cut(s) 269, 270, 1496, 1497
BcoDI GTCTC 6 cut(s) 569, 728, 1519, 1779, 2110, 2296
BfaI CTAG 5 cut(s) 591, 810, 869, 1148, 1422
BfoI RGCGCY 1 cut(s) 416
BfuI GTATCC 1 cut(s) 1966
BglII AGATCT 2 cut(s) 201, 1851
BisI GCNGC 4 cut(s) 641, 1554, 1994, 2483
BlsI GCNGC 4 cut(s) 642, 1555, 1995, 2484
Bme1390I CCNGG 5 cut(s) 269, 270, 1496, 1497, 1560
Bme18I GGWCC 1 cut(s) 2078
BmeT110I CYCGRG 4 cut(s) 80, 268, 1495, 2125
BmgT120I GGNCC 1 cut(s) 2078
BmiI GGNNCC 5 cut(s) 35, 265, 807, 1044, 2187
BmrFI CCNGG 5 cut(s) 269, 270, 1496, 1497, 1560
BmsI GCATC 5 cut(s) 556, 1156, 1256, 1860, 2465
BmtI GCTAGC 1 cut(s) 1425
BpiI GAAGAC 2 cut(s) 1313, 1522
BpmI CTGGAG 1 cut(s) 2320
Bpu10I CCTNAGC 1 cut(s) 2412
Bpu14I TTCGAA 3 cut(s) 1067, 1078, 2210
BpuEI CTTGAG 4 cut(s) 563, 620, 942, 1883
BpuMI CCSGG 4 cut(s) 269, 270, 1496, 1497
Bsa29I ATCGAT 1 cut(s) 790
BsaAI YACGTR 1 cut(s) 286
BsaJI CCNNGG 9 cut(s) 58, 267, 268, 431, 1299, 1495, 1496, 1538, 2071
BsaWI WCCGGW 3 cut(s) 1602, 1745, 2177
Bsc4I CCNNNNNNNGG 6 cut(s) 884, 1039, 1052, 1900, 2132, 2377
Bse1I ACTGG 3 cut(s) 889, 2303, 2331
Bse21I CCTNAGG 1 cut(s) 645
Bse3DI GCAATG 2 cut(s) 34, 2137
BseBI CCWGG 1 cut(s) 1560
BseCI ATCGAT 1 cut(s) 790
BseDI CCNNGG 9 cut(s) 58, 267, 268, 431, 1299, 1495, 1496, 1538, 2071
BseGI GGATG 6 cut(s) 139, 710, 1171, 1237, 1573, 2104
BseLI CCNNNNNNNGG 6 cut(s) 884, 1039, 1052, 1900, 2132, 2377
BseMI GCAATG 2 cut(s) 34, 2137
BseMII CTCAG 9 cut(s) 230, 458, 688, 992, 1292, 1746, 1869, 2076, 2403
BseNI ACTGG 3 cut(s) 889, 2303, 2331
BseRI GAGGAG 2 cut(s) 2409, 2412
BseXI GCAGC 3 cut(s) 627, 1565, 2005
BsgI GTGCAG 2 cut(s) 1983, 2012
Bsh1236I CGCG 4 cut(s) 60, 1565, 1970, 2485
BshFI GGCC 3 cut(s) 465, 963, 2330
BshVI ATCGAT 1 cut(s) 790
BsiHKAI GWGCWC 1 cut(s) 332
BsiHKCI CYCGRG 4 cut(s) 80, 268, 1495, 2125
BsiSI CCGG 6 cut(s) 269, 1496, 1518, 1603, 1746, 2178
BslFI GGGAC 2 cut(s) 63, 1478
BslI CCNNNNNNNGG 6 cut(s) 884, 1039, 1052, 1900, 2132, 2377
BsmAI GTCTC 6 cut(s) 569, 728, 1519, 1779, 2110, 2296
BsmFI GGGAC 2 cut(s) 63, 1478
BsmI GAATGC 2 cut(s) 1873, 2243
BsnI GGCC 3 cut(s) 465, 963, 2330
BsoBI CYCGRG 4 cut(s) 80, 268, 1495, 2125
Bsp119I TTCGAA 3 cut(s) 1067, 1078, 2210
Bsp1286I GDGCHC 1 cut(s) 332
BspANI GGCC 3 cut(s) 465, 963, 2330
BspCNI CTCAG 9 cut(s) 229, 459, 687, 991, 1291, 1747, 1868, 2077, 2404
BspDI ATCGAT 1 cut(s) 790
BspFNI CGCG 4 cut(s) 60, 1565, 1970, 2485
BspLI GGNNCC 5 cut(s) 35, 265, 807, 1044, 2187
BspOI GCTAGC 1 cut(s) 1425
BspPI GGATC 9 cut(s) 25, 28, 41, 767, 1343, 1529, 1925, 2180, 2193
BspT104I TTCGAA 3 cut(s) 1067, 1078, 2210
BsrDI GCAATG 2 cut(s) 34, 2137
BsrI ACTGG 3 cut(s) 889, 2303, 2331
BssECI CCNNGG 9 cut(s) 58, 267, 268, 431, 1299, 1495, 1496, 1538, 2071
BssSI CACGAG 2 cut(s) 521, 1799
BssT1I CCWWGG 3 cut(s) 431, 1299, 2071
Bst2BI CACGAG 2 cut(s) 521, 1799
Bst2UI CCWGG 1 cut(s) 1560
Bst4CI ACNGT 3 cut(s) 1492, 1949, 2026
Bst6I CTCTTC 5 cut(s) 304, 365, 432, 1842, 2430
BstAPI GCANNNNNTGC 1 cut(s) 1289
BstBAI YACGTR 1 cut(s) 286
BstBI TTCGAA 3 cut(s) 1067, 1078, 2210
BstC8I GCNNGC 3 cut(s) 332, 1423, 1998
BstDSI CCRYGG 1 cut(s) 58
BstF5I GGATG 6 cut(s) 139, 710, 1171, 1237, 1573, 2104
BstFNI CGCG 4 cut(s) 60, 1565, 1970, 2485
BstH2I RGCGCY 1 cut(s) 416
BstHHI GCGC 3 cut(s) 415, 719, 1565
BstMAI GTCTC 6 cut(s) 569, 728, 1519, 1779, 2110, 2296
BstMWI GCNNNNNNNGC 6 cut(s) 195, 493, 1289, 1482, 1562, 2479
BstNI CCWGG 1 cut(s) 1560
BstNSI RCATGY 1 cut(s) 1298
BstSCI CCNGG 5 cut(s) 267, 268, 1494, 1495, 1558
BstUI CGCG 4 cut(s) 60, 1565, 1970, 2485
BstV1I GCAGC 3 cut(s) 627, 1565, 2005
BstV2I GAAGAC 2 cut(s) 1313, 1522
BstX2I RGATCY 4 cut(s) 33, 201, 1851, 2185
BstYI RGATCY 4 cut(s) 33, 201, 1851, 2185
Bsu15I ATCGAT 1 cut(s) 790
Bsu36I CCTNAGG 1 cut(s) 645
BsuI GTATCC 1 cut(s) 1966
BsuRI GGCC 3 cut(s) 465, 963, 2330
BsuTUI ATCGAT 1 cut(s) 790
BtgI CCRYGG 1 cut(s) 58
BtgZI GCGATG 2 cut(s) 59, 1534
BtsCI GGATG 6 cut(s) 139, 710, 1171, 1237, 1573, 2104
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 1 cut(s) 231
Cac8I GCNNGC 3 cut(s) 332, 1423, 1998
CaiI CAGNNNCTG 2 cut(s) 1559, 2326
CfoI GCGC 3 cut(s) 415, 719, 1565
Cfr13I GGNCC 1 cut(s) 2078
Cfr42I CCGCGG 1 cut(s) 61
Cfr9I CCCGGG 2 cut(s) 268, 1495
ClaI ATCGAT 1 cut(s) 790
CseI GACGC 2 cut(s) 1019, 1832
Csp6I GTAC 1 cut(s) 2286
CviAII CATG 7 cut(s) 181, 1255, 1295, 1480, 2038, 2247, 2278
CviQI GTAC 1 cut(s) 2286
DraI TTTAAA 1 cut(s) 501
EaeI YGGCCR 1 cut(s) 2328
Eam1104I CTCTTC 5 cut(s) 304, 365, 432, 1842, 2430
EarI CTCTTC 5 cut(s) 304, 365, 432, 1842, 2430
Eco130I CCWWGG 3 cut(s) 431, 1299, 2071
Eco147I AGGCCT 1 cut(s) 963
Eco32I GATATC 2 cut(s) 1135, 1330
Eco47I GGWCC 1 cut(s) 2078
Eco47III AGCGCT 1 cut(s) 414
Eco81I CCTNAGG 1 cut(s) 645
Eco88I CYCGRG 4 cut(s) 80, 268, 1495, 2125
EcoRI GAATTC 1 cut(s) 1080
EcoRII CCWGG 1 cut(s) 1558
EcoRV GATATC 2 cut(s) 1135, 1330
EcoT14I CCWWGG 3 cut(s) 431, 1299, 2071
EcoT22I ATGCAT 2 cut(s) 394, 1875
ErhI CCWWGG 3 cut(s) 431, 1299, 2071
FaeI CATG 7 cut(s) 184, 1258, 1298, 1483, 2041, 2250, 2281
FalI AAGNNNNNCTT 4 cut(s) 177, 209, 1969, 2001
FaqI GGGAC 2 cut(s) 63, 1478
FatI CATG 7 cut(s) 180, 1254, 1294, 1479, 2037, 2246, 2277
FauI CCCGC 3 cut(s) 53, 519, 1133
Fnu4HI GCNGC 4 cut(s) 641, 1554, 1994, 2483
FokI GGATG 6 cut(s) 146, 717, 1178, 1244, 1580, 2111
Fsp4HI GCNGC 4 cut(s) 641, 1554, 1994, 2483
FspBI CTAG 5 cut(s) 591, 810, 869, 1148, 1422
GlaI GCGC 3 cut(s) 414, 718, 1564
GluI GCNGC 4 cut(s) 641, 1554, 1994, 2483
GsuI CTGGAG 1 cut(s) 2320
HaeII RGCGCY 1 cut(s) 416
HaeIII GGCC 3 cut(s) 465, 963, 2330
HapII CCGG 6 cut(s) 269, 1496, 1518, 1603, 1746, 2178
HgaI GACGC 2 cut(s) 1019, 1832
HhaI GCGC 3 cut(s) 415, 719, 1565
Hin1II CATG 7 cut(s) 184, 1258, 1298, 1483, 2041, 2250, 2281
Hin6I GCGC 3 cut(s) 413, 717, 1563
HinP1I GCGC 3 cut(s) 413, 717, 1563
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 3 cut(s) 560, 596, 1265
HpaI GTTAAC 1 cut(s) 295
HpaII CCGG 6 cut(s) 269, 1496, 1518, 1603, 1746, 2178
HphI GGTGA 6 cut(s) 121, 1603, 1804, 1961, 2392, 2455
Hpy166II GTNNAC 7 cut(s) 295, 526, 841, 901, 1687, 1795, 1952
Hpy8I GTNNAC 7 cut(s) 295, 526, 841, 901, 1687, 1795, 1952
Hpy99I CGWCG 3 cut(s) 368, 1116, 1826
HpyAV CCTTC 6 cut(s) 99, 165, 179, 1190, 2210, 2362
HpyCH4III ACNGT 3 cut(s) 1492, 1949, 2026
HpyCH4IV ACGT 5 cut(s) 73, 243, 285, 822, 1111
HpyCH4V TGCA 8 cut(s) 392, 1029, 1169, 1292, 1873, 1993, 2000, 2261
HpyF10VI GCNNNNNNNGC 6 cut(s) 195, 493, 1289, 1482, 1562, 2479
HpySE526I ACGT 5 cut(s) 73, 243, 285, 822, 1111
Hsp92II CATG 7 cut(s) 184, 1258, 1298, 1483, 2041, 2250, 2281
HspAI GCGC 3 cut(s) 413, 717, 1563
KspAI GTTAAC 1 cut(s) 295
KspI CCGCGG 1 cut(s) 61
LmnI GCTCC 3 cut(s) 410, 637, 1561
Lsp1109I GCAGC 3 cut(s) 627, 1565, 2005
LweI GCATC 5 cut(s) 556, 1156, 1256, 1860, 2465
MaeI CTAG 5 cut(s) 591, 810, 869, 1148, 1422
MaeII ACGT 5 cut(s) 73, 243, 285, 822, 1111
MaeIII GTNAC 5 cut(s) 160, 537, 991, 1090, 2053
MflI RGATCY 4 cut(s) 33, 201, 1851, 2185
MhlI GDGCHC 1 cut(s) 332
MlsI TGGCCA 1 cut(s) 2330
MluNI TGGCCA 1 cut(s) 2330
MlyI GAGTC 3 cut(s) 186, 571, 1565
MmeI TCCRAC 2 cut(s) 732, 1020
Mox20I TGGCCA 1 cut(s) 2330
Mph1103I ATGCAT 2 cut(s) 394, 1875
MroXI GAANNNNTTC 1 cut(s) 939
MscI TGGCCA 1 cut(s) 2330
Msp20I TGGCCA 1 cut(s) 2330
MspA1I CMGCKG 1 cut(s) 60
MspI CCGG 6 cut(s) 269, 1496, 1518, 1603, 1746, 2178
MspR9I CCNGG 5 cut(s) 269, 270, 1496, 1497, 1560
MssI GTTTAAAC 1 cut(s) 501
Mva1269I GAATGC 2 cut(s) 1873, 2243
MvaI CCWGG 1 cut(s) 1560
MvnI CGCG 4 cut(s) 60, 1565, 1970, 2485
MwoI GCNNNNNNNGC 6 cut(s) 195, 493, 1289, 1482, 1562, 2479
NciI CCSGG 4 cut(s) 269, 270, 1496, 1497
NheI GCTAGC 1 cut(s) 1421
NlaIII CATG 7 cut(s) 184, 1258, 1298, 1483, 2041, 2250, 2281
NlaIV GGNNCC 5 cut(s) 35, 265, 807, 1044, 2187
NmuCI GTSAC 2 cut(s) 537, 2053
NsiI ATGCAT 2 cut(s) 394, 1875
NspI RCATGY 1 cut(s) 1298
NspV TTCGAA 3 cut(s) 1067, 1078, 2210
PaeR7I CTCGAG 1 cut(s) 80
PceI AGGCCT 1 cut(s) 963
PcsI WCGNNNNNNNCGW 1 cut(s) 1064
PctI GAATGC 2 cut(s) 1873, 2243
PdmI GAANNNNTTC 1 cut(s) 939
PflMI CCANNNNNTGG 1 cut(s) 1039
PkrI GCNGC 4 cut(s) 642, 1555, 1995, 2484
PleI GAGTC 3 cut(s) 185, 571, 1565
PmeI GTTTAAAC 1 cut(s) 501
PpsI GAGTC 3 cut(s) 185, 571, 1565
Ppu21I YACGTR 1 cut(s) 286
PshBI ATTAAT 2 cut(s) 1001, 1524
Psp1406I AACGTT 2 cut(s) 73, 822
Psp6I CCWGG 1 cut(s) 1558
PspGI CCWGG 1 cut(s) 1558
PspN4I GGNNCC 5 cut(s) 35, 265, 807, 1044, 2187
PspPI GGNCC 1 cut(s) 2078
PstNI CAGNNNCTG 2 cut(s) 1559, 2326
PsuI RGATCY 4 cut(s) 33, 201, 1851, 2185
RsaI GTAC 1 cut(s) 2287
RsaNI GTAC 1 cut(s) 2286
SacII CCGCGG 1 cut(s) 61
SatI GCNGC 4 cut(s) 641, 1554, 1994, 2483
Sau96I GGNCC 1 cut(s) 2078
SchI GAGTC 3 cut(s) 186, 571, 1565
ScrFI CCNGG 5 cut(s) 269, 270, 1496, 1497, 1560
SduI GDGCHC 1 cut(s) 332
SfaNI GCATC 5 cut(s) 556, 1156, 1256, 1860, 2465
Sfr274I CTCGAG 1 cut(s) 80
Sfr303I CCGCGG 1 cut(s) 61
SfuI TTCGAA 3 cut(s) 1067, 1078, 2210
SgrBI CCGCGG 1 cut(s) 61
SinI GGWCC 1 cut(s) 2078
SlaI CTCGAG 1 cut(s) 80
SmaI CCCGGG 2 cut(s) 270, 1497
SmlI CTYRAG 5 cut(s) 80, 542, 599, 957, 1898
SmoI CTYRAG 5 cut(s) 80, 542, 599, 957, 1898
SseBI AGGCCT 1 cut(s) 963
SspI AATATT 3 cut(s) 67, 1064, 1072
SspMI CTAG 5 cut(s) 591, 810, 869, 1148, 1422
StuI AGGCCT 1 cut(s) 963
StyD4I CCNGG 5 cut(s) 267, 268, 1494, 1495, 1558
StyI CCWWGG 3 cut(s) 431, 1299, 2071
TaaI ACNGT 3 cut(s) 1492, 1949, 2026
TaiI ACGT 5 cut(s) 76, 246, 288, 825, 1114
TatI WGTACW 1 cut(s) 2285
TauI GCSGC 1 cut(s) 2485
TscAI CASTG 1 cut(s) 231
TseFI GTSAC 2 cut(s) 537, 2053
TseI GCWGC 3 cut(s) 640, 1553, 1993
Tsp45I GTSAC 2 cut(s) 537, 2053
TspGWI ACGGA 1 cut(s) 1244
TspMI CCCGGG 2 cut(s) 268, 1495
TspRI CASTG 1 cut(s) 231
Van91I CCANNNNNTGG 1 cut(s) 1039
VpaK11BI GGWCC 1 cut(s) 2078
VspI ATTAAT 2 cut(s) 1001, 1524
XapI RAATTY 2 cut(s) 1080, 2374
XceI RCATGY 1 cut(s) 1298
XhoI CTCGAG 1 cut(s) 80
XmaI CCCGGG 2 cut(s) 268, 1495
XmnI GAANNNNTTC 1 cut(s) 939
XspI CTAG 5 cut(s) 591, 810, 869, 1148, 1422
Zsp2I ATGCAT 2 cut(s) 394, 1875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.