Rorug03G0240100

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004012
Physical Location & Seq
Forward (+)
22458398 .. 22460237
1840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0240100.1

Sequence Viewer

Length: 1185 bp
ATGGCTCAAGAAATGGAACTGCACTTGAAAGACAAGTTCCTGGACGGCCTTGATATGAAGGACAAAAGCATCTCCAAGATTCCAAGGTACAACCAATTTCAAGAGATAAAGAGATTGCTAAAAGACATGTTCAGCTCATCCACGCCTGCAATAGTCAAGTTTCCGAGTGCAACCAATTTCAGGGACAAGCTTTACAAGCTCAACAATCTTTTGACCGACTGCAAGATGCTATCAAGAAAACATGGTTTCAACTCTCCGGAGGAGCTAATTACTATTAACAGAATCCGGAGAGACTTAAGACAGATCAAGAGGGATCTCCAGGCCATTACGAAGGATGCATCAAATCGAGACATTAATGAACAACGTATTAATGGTGATTCAAGCTCACATGATAGAGACATTTTGAGATGGACTACTCGTGCAGTAGATGCAACAAAGGTTTATGGACTTGATGATGAGGTGTTGTCCATGGAAAACTTGCTTCTCAAGAAAGGAAGTGATGTTCATCAGTTCAAGGCAATAGGCATTGTAGGCAGGGATGGTATAGGAAAGACAACGCTTTGCCAACTGATGTTCAACAAAGATGAAGTGAAAAACAACTTCAATCCAAGGATCTGGGTATGCATGGCCAGACACCCGGATGACGACGAGGATGAGGATCTTAAATTAGCAATTGTCAAAAGAATGTTGAGGTACCTTGGAGTCGAAGAGGCAATAGTCAAGTCGATTTGTGATGTAAAACCTGGCCTAGAAGGACTGCTATGTGCTCTTTATCTGCAATTGTTGGGCAAGAGGTATCTAATTGTGCTTGATGATGCTAGGGAGACAGACTCATGGTATAAAGAGTTGGATTCCAGTTTGACTCATGATAAGAAATGGGGTGACAGTTTCGCATTTGGATTTCCAAAAGGCAGTGGAGGAAGAGTCATAGTTACAAGCAGAAATGAAGACATAGGAAATATGATGGTTGGGAAGGAAAATATACATCACCTTGAGCCCATGTTGGACACTGAAAGCTGCTGGGCAATATTCAAAGACTCGGTTGACGAATATCTGTTACCTACCATTCCCTCAATCTTGGAAGAACTAAAATCAGAAGTAAAACAAAAATGTGGTGGCCTTCCGTTGGCTGCAAAAATGATGGGGCGTGCAATGCAGGAGGAAGAAGAGCGTACTAGAAGCTAG

Protein Analysis

394

Amino Acids

45.19

Weight (kDa)

6.49

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 168 - 348 5.3e-21 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 693
AccB1I GGYRCC 1 cut(s) 693
AccIII TCCGGA 2 cut(s) 256, 285
AclWI GGATC 3 cut(s) 321, 620, 666
AcoI YGGCCR 1 cut(s) 627
AfaI GTAC 3 cut(s) 89, 695, 1174
AfiI CCNNNNNNNGG 2 cut(s) 180, 1126
AflII CTTAAG 1 cut(s) 295
AflIII ACRYGT 1 cut(s) 126
AgsI TTSAA 8 cut(s) 28, 101, 250, 381, 514, 577, 604, 1033
AjnI CCWGG 3 cut(s) 39, 318, 742
AloI GAACNNNNNNTCC 2 cut(s) 486, 518
AluBI AGCT 7 cut(s) 135, 190, 199, 265, 384, 1017, 1182
AluI AGCT 7 cut(s) 135, 190, 199, 265, 384, 1017, 1182
Alw21I GWGCWC 1 cut(s) 769
Alw26I GTCTC 4 cut(s) 285, 342, 390, 818
AlwI GGATC 3 cut(s) 321, 620, 666
Aor13HI TCCGGA 2 cut(s) 256, 285
AoxI GGCC 5 cut(s) 46, 321, 627, 745, 1117
ApeKI GCWGC 2 cut(s) 1017, 1130
AseI ATTAAT 2 cut(s) 354, 369
Asp718I GGTACC 1 cut(s) 693
AsuC2I CCSGG 1 cut(s) 638
AsuHPI GGTGA 3 cut(s) 386, 893, 980
BalI TGGCCA 1 cut(s) 629
BanI GGYRCC 1 cut(s) 693
BanII GRGCYC 1 cut(s) 999
BauI CACGAG 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 954
Bbv12I GWGCWC 1 cut(s) 769
BbvI GCAGC 2 cut(s) 1004, 1117
BccI CCATC 4 cut(s) 402, 533, 958, 1135
BceAI ACGGC 1 cut(s) 61
BciT130I CCWGG 3 cut(s) 41, 320, 744
BcnI CCSGG 1 cut(s) 638
BcoDI GTCTC 4 cut(s) 285, 342, 390, 818
BfaI CTAG 4 cut(s) 749, 819, 1176, 1183
BfrI CTTAAG 1 cut(s) 295
BisI GCNGC 2 cut(s) 1018, 1131
BlsI GCNGC 2 cut(s) 1019, 1132
Bme1390I CCNGG 4 cut(s) 41, 320, 638, 744
BmiI GGNNCC 1 cut(s) 695
BmrFI CCNGG 4 cut(s) 41, 320, 638, 744
BmsI GCATC 6 cut(s) 78, 216, 325, 347, 418, 805
BpiI GAAGAC 1 cut(s) 954
BplI GAGNNNNNCTC 2 cut(s) 815, 847
BpmI CTGGAG 1 cut(s) 302
BpuEI CTTGAG 2 cut(s) 470, 1013
BpuMI CCSGG 1 cut(s) 638
BsaBI GATNNNNATC 2 cut(s) 504, 657
BsaJI CCNNGG 4 cut(s) 83, 468, 608, 697
BsaWI WCCGGW 2 cut(s) 256, 285
BsaXI ACNNNNNCTCC 2 cut(s) 909, 939
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 1126
Bse1I ACTGG 1 cut(s) 855
Bse3DI GCAATG 1 cut(s) 1158
Bse8I GATNNNNATC 2 cut(s) 504, 657
BseAI TCCGGA 2 cut(s) 256, 285
BseBI CCWGG 3 cut(s) 41, 320, 744
BseDI CCNNGG 4 cut(s) 83, 468, 608, 697
BseGI GGATG 5 cut(s) 137, 340, 544, 646, 658
BseJI GATNNNNATC 2 cut(s) 504, 657
BseLI CCNNNNNNNGG 2 cut(s) 180, 1126
BseMI GCAATG 1 cut(s) 1158
BseNI ACTGG 1 cut(s) 855
BseRI GAGGAG 1 cut(s) 275
BseXI GCAGC 2 cut(s) 1004, 1117
BseYI CCCAGC 1 cut(s) 1020
BsgI GTGCAG 2 cut(s) 5, 441
BshFI GGCC 5 cut(s) 48, 323, 629, 747, 1119
BshNI GGYRCC 1 cut(s) 693
BsiHKAI GWGCWC 1 cut(s) 769
BsiSI CCGG 3 cut(s) 257, 286, 638
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 2 cut(s) 180, 1126
BsmAI GTCTC 4 cut(s) 285, 342, 390, 818
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 5 cut(s) 48, 323, 629, 747, 1119
Bsp1286I GDGCHC 2 cut(s) 769, 999
Bsp13I TCCGGA 2 cut(s) 256, 285
Bsp143I GATC 4 cut(s) 303, 313, 612, 658
Bsp19I CCATGG 1 cut(s) 468
BspANI GGCC 5 cut(s) 48, 323, 629, 747, 1119
BspEI TCCGGA 2 cut(s) 256, 285
BspHI TCATGA 1 cut(s) 865
BspLI GGNNCC 1 cut(s) 695
BspPI GGATC 3 cut(s) 321, 620, 666
BspQI GCTCTTC 1 cut(s) 1161
BspT107I GGYRCC 1 cut(s) 693
BspTI CTTAAG 1 cut(s) 295
BsrDI GCAATG 1 cut(s) 1158
BsrI ACTGG 1 cut(s) 855
BssECI CCNNGG 4 cut(s) 83, 468, 608, 697
BssMI GATC 4 cut(s) 303, 313, 612, 658
BssSI CACGAG 1 cut(s) 417
BssT1I CCWWGG 4 cut(s) 83, 468, 608, 697
Bst2BI CACGAG 1 cut(s) 417
Bst2UI CCWGG 3 cut(s) 41, 320, 744
Bst4CI ACNGT 1 cut(s) 887
Bst6I CTCTTC 3 cut(s) 702, 916, 1161
BstAFI CTTAAG 1 cut(s) 295
BstAPI GCANNNNNTGC 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 147, 1149
BstDSI CCRYGG 1 cut(s) 468
BstF5I GGATG 5 cut(s) 137, 340, 544, 646, 658
BstKTI GATC 4 cut(s) 306, 316, 615, 661
BstMAI GTCTC 4 cut(s) 285, 342, 390, 818
BstMBI GATC 4 cut(s) 303, 313, 612, 658
BstMWI GCNNNNNNNGC 4 cut(s) 196, 428, 531, 1153
BstNI CCWGG 3 cut(s) 41, 320, 744
BstNSI RCATGY 1 cut(s) 130
BstSCI CCNGG 4 cut(s) 39, 318, 636, 742
BstV1I GCAGC 2 cut(s) 1004, 1117
BstV2I GAAGAC 1 cut(s) 954
BstX2I RGATCY 3 cut(s) 313, 612, 658
BstXI CCANNNNNNTGG 1 cut(s) 615
BstYI RGATCY 3 cut(s) 313, 612, 658
BsuRI GGCC 5 cut(s) 48, 323, 629, 747, 1119
BtgI CCRYGG 1 cut(s) 468
BtsCI GGATG 5 cut(s) 137, 340, 544, 646, 658
BtsI GCAGTG 1 cut(s) 919
BtsIMutI CAGTG 2 cut(s) 919, 1008
Cac8I GCNNGC 2 cut(s) 147, 1149
CciI TCATGA 1 cut(s) 865
Csp6I GTAC 3 cut(s) 88, 694, 1173
CviAII CATG 8 cut(s) 127, 242, 389, 469, 625, 834, 866, 1000
CviQI GTAC 3 cut(s) 88, 694, 1173
DpnI GATC 4 cut(s) 305, 315, 614, 660
DpnII GATC 4 cut(s) 303, 313, 612, 658
EaeI YGGCCR 1 cut(s) 627
Eam1104I CTCTTC 3 cut(s) 702, 916, 1161
EarI CTCTTC 3 cut(s) 702, 916, 1161
Eco130I CCWWGG 4 cut(s) 83, 468, 608, 697
Eco24I GRGCYC 1 cut(s) 999
EcoRII CCWGG 3 cut(s) 39, 318, 742
EcoT14I CCWWGG 4 cut(s) 83, 468, 608, 697
EcoT22I ATGCAT 2 cut(s) 340, 626
EcoT38I GRGCYC 1 cut(s) 999
ErhI CCWWGG 4 cut(s) 83, 468, 608, 697
FaeI CATG 8 cut(s) 130, 245, 392, 472, 628, 837, 869, 1003
FaqI GGGAC 1 cut(s) 197
FatI CATG 8 cut(s) 126, 241, 388, 468, 624, 833, 865, 999
Fnu4HI GCNGC 2 cut(s) 1018, 1131
FokI GGATG 5 cut(s) 124, 347, 551, 653, 665
FriOI GRGCYC 1 cut(s) 999
Fsp4HI GCNGC 2 cut(s) 1018, 1131
FspBI CTAG 4 cut(s) 749, 819, 1176, 1183
GluI GCNGC 2 cut(s) 1018, 1131
GsaI CCCAGC 1 cut(s) 1024
GsuI CTGGAG 1 cut(s) 302
HaeIII GGCC 5 cut(s) 48, 323, 629, 747, 1119
HapII CCGG 3 cut(s) 257, 286, 638
Hin1II CATG 8 cut(s) 130, 245, 392, 472, 628, 837, 869, 1003
HincII GTYRAC 1 cut(s) 1045
HindII GTYRAC 1 cut(s) 1045
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 9 cut(s) 79, 282, 377, 702, 830, 851, 862, 924, 1037
HpaII CCGG 3 cut(s) 257, 286, 638
HphI GGTGA 3 cut(s) 386, 893, 980
Hpy166II GTNNAC 1 cut(s) 1045
Hpy188I TCNGA 2 cut(s) 165, 1096
Hpy188III TCNNGA 9 cut(s) 8, 101, 234, 257, 286, 307, 347, 487, 866
Hpy8I GTNNAC 1 cut(s) 1045
Hpy99I CGWCG 1 cut(s) 650
HpyAV CCTTC 5 cut(s) 52, 325, 746, 967, 1130
HpyCH4III ACNGT 1 cut(s) 887
HpyCH4IV ACGT 1 cut(s) 364
HpyF10VI GCNNNNNNNGC 4 cut(s) 196, 428, 531, 1153
HpySE526I ACGT 1 cut(s) 364
Hsp92II CATG 8 cut(s) 130, 245, 392, 472, 628, 837, 869, 1003
Kpn2I TCCGGA 2 cut(s) 256, 285
KpnI GGTACC 1 cut(s) 697
Kzo9I GATC 4 cut(s) 303, 313, 612, 658
LguI GCTCTTC 1 cut(s) 1161
LmnI GCTCC 1 cut(s) 262
Lsp1109I GCAGC 2 cut(s) 1004, 1117
LweI GCATC 6 cut(s) 78, 216, 325, 347, 418, 805
MaeI CTAG 4 cut(s) 749, 819, 1176, 1183
MaeII ACGT 1 cut(s) 364
MaeIII GTNAC 3 cut(s) 881, 931, 1056
MalI GATC 4 cut(s) 305, 315, 614, 660
MboI GATC 4 cut(s) 303, 313, 612, 658
MboII GAAGA 6 cut(s) 719, 933, 959, 1094, 1175, 1178
MfeI CAATTG 2 cut(s) 672, 779
MflI RGATCY 3 cut(s) 313, 612, 658
MhlI GDGCHC 2 cut(s) 769, 999
MlsI TGGCCA 1 cut(s) 629
MluCI AATT 7 cut(s) 95, 175, 267, 665, 672, 779, 801
MluNI TGGCCA 1 cut(s) 629
MlyI GAGTC 5 cut(s) 711, 824, 856, 933, 1031
MmeI TCCRAC 2 cut(s) 828, 984
Mox20I TGGCCA 1 cut(s) 629
Mph1103I ATGCAT 2 cut(s) 340, 626
MroI TCCGGA 2 cut(s) 256, 285
MscI TGGCCA 1 cut(s) 629
MseI TTAA 5 cut(s) 276, 296, 354, 369, 663
MslI CAYNNNNRTG 1 cut(s) 639
Msp20I TGGCCA 1 cut(s) 629
MspCI CTTAAG 1 cut(s) 295
MspI CCGG 3 cut(s) 257, 286, 638
MspR9I CCNGG 4 cut(s) 41, 320, 638, 744
MunI CAATTG 2 cut(s) 672, 779
MvaI CCWGG 3 cut(s) 41, 320, 744
MwoI GCNNNNNNNGC 4 cut(s) 196, 428, 531, 1153
NciI CCSGG 1 cut(s) 638
NcoI CCATGG 1 cut(s) 468
NdeII GATC 4 cut(s) 303, 313, 612, 658
NlaIII CATG 8 cut(s) 130, 245, 392, 472, 628, 837, 869, 1003
NlaIV GGNNCC 1 cut(s) 695
NmuCI GTSAC 1 cut(s) 881
NsiI ATGCAT 2 cut(s) 340, 626
NspI RCATGY 1 cut(s) 130
PagI TCATGA 1 cut(s) 865
PciI ACATGT 1 cut(s) 126
PciSI GCTCTTC 1 cut(s) 1161
PfeI GAWTC 4 cut(s) 79, 282, 377, 851
PfoI TCCNGGA 1 cut(s) 39
PkrI GCNGC 2 cut(s) 1019, 1132
PleI GAGTC 5 cut(s) 710, 824, 856, 932, 1031
PpsI GAGTC 5 cut(s) 710, 824, 856, 932, 1031
PscI ACATGT 1 cut(s) 126
PshBI ATTAAT 2 cut(s) 354, 369
Psp6I CCWGG 3 cut(s) 39, 318, 742
PspFI CCCAGC 1 cut(s) 1020
PspGI CCWGG 3 cut(s) 39, 318, 742
PspN4I GGNNCC 1 cut(s) 695
PsuI RGATCY 3 cut(s) 313, 612, 658
RsaI GTAC 3 cut(s) 89, 695, 1174
RsaNI GTAC 3 cut(s) 88, 694, 1173
RseI CAYNNNNRTG 1 cut(s) 639
SapI GCTCTTC 1 cut(s) 1161
SaqAI TTAA 5 cut(s) 276, 296, 354, 369, 663
SatI GCNGC 2 cut(s) 1018, 1131
Sau3AI GATC 4 cut(s) 303, 313, 612, 658
SchI GAGTC 5 cut(s) 711, 824, 856, 933, 1031
ScrFI CCNGG 4 cut(s) 41, 320, 638, 744
SduI GDGCHC 2 cut(s) 769, 999
SfaNI GCATC 6 cut(s) 78, 216, 325, 347, 418, 805
SmiMI CAYNNNNRTG 1 cut(s) 639
SmlI CTYRAG 4 cut(s) 6, 295, 485, 992
SmoI CTYRAG 4 cut(s) 6, 295, 485, 992
Sse9I AATT 7 cut(s) 95, 175, 267, 665, 672, 779, 801
SspI AATATT 1 cut(s) 1029
SspMI CTAG 4 cut(s) 749, 819, 1176, 1183
StyD4I CCNGG 4 cut(s) 39, 318, 636, 742
StyI CCWWGG 4 cut(s) 83, 468, 608, 697
TaaI ACNGT 1 cut(s) 887
TaiI ACGT 1 cut(s) 367
TaqI TCGA 3 cut(s) 346, 705, 725
TaqII GACCGA 1 cut(s) 230
TasI AATT 7 cut(s) 95, 175, 267, 665, 672, 779, 801
TfiI GAWTC 4 cut(s) 79, 282, 377, 851
Tru1I TTAA 5 cut(s) 276, 296, 354, 369, 663
Tru9I TTAA 5 cut(s) 276, 296, 354, 369, 663
TscAI CASTG 2 cut(s) 919, 1015
TseFI GTSAC 1 cut(s) 881
TseI GCWGC 2 cut(s) 1017, 1130
Tsp45I GTSAC 1 cut(s) 881
TspDTI ATGAA 5 cut(s) 71, 372, 494, 600, 960
TspGWI ACGGA 1 cut(s) 1113
TspRI CASTG 2 cut(s) 919, 1015
Vha464I CTTAAG 1 cut(s) 295
VspI ATTAAT 2 cut(s) 354, 369
XceI RCATGY 1 cut(s) 130
XspI CTAG 4 cut(s) 749, 819, 1176, 1183
Zsp2I ATGCAT 2 cut(s) 340, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.