MD09G1280200.v1.1

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004012
Physical Location & Seq
Reverse (-)
35695029 .. 35699586
4558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1280200.v1.1.491

Sequence Viewer

Length: 2502 bp
ATGGCTTCTGCAGCAGCTATCAAAGGCTCCGAGTCCATAGCTGATAACATGCCGGATGCCCGAAGGCAGAGCCGGTACCACATGAAGAGGTGCTTCGCTAAGTACGTCGAGAAAGGAAGAAGGATAATAAAGCTCCACCATTTGATGAGTGAAATGGAGACAGTCATCGATGACAAAGCTGAAAGAACCCAAGTTTTGGAGGGTGTTCTTGGCTATATATTATGTTCAACTCAGGAAGCTGTTGTTATTCCTCCACATGTTGTCTTTTCAATAAGACCAAATCCAGGATACTGGGAATTTGTTAAGGTCAGCTCTGAGGACCTCTCAATTGAGGGCATCACTGTCAGAGACTTCTTGAAATACAAAGAAACTTTATACGATGAGAATTGGTCAAATGATGAAAATGCACTTGAGGTAGATTTTAGAGCAATTGATTTCTCTACTCCTCACTTAACTCTATCTTCCTCGATCGGAAATGGAATCAATTATGTTACCAAGTTCACCACTTCAAAGCTAGCTGGAAAACTGGAGAATGCACAACCCCTTGTGGATTACTTACTTTCACTAAATCATCAAGGAGAACAACTTATTCTAAATGAGACCCTCAACACCGCTTCAAAGCTTCAGGCAGCACTGATTGTAACAGATGTGCACCTCTCAGCGCTTCCTAAGGACACACCATTCCAAAATTTTGAACTAAGGTTTAAGGAGTGGGGCTTTGAGAAGGGTTGGGGAGACACTGCAGAAAGAACAAAGGAGACAATGGGAATACTTGCGGAAGTACTACAGGCTCCAGACCCGTTGATCATGGACAGATTTTTCAGTAGGCTTCCCACAATATTCAATGTTGTAATATTCTCTCCTCATGGCTACTTTGGTCAAGCAGATGTTCTTGGCTTGCCAGACACTGGTGGGCAGGTAGTTTACATTCTTGATCAAGTGAAAGCTTTGGAGGAAGAATTGCTTGTGAGAATTAAGCAACAAGGACTTACTGTGAAGCCTCAAATTCTTGTCGTGACACGACTCATACCAGAAGCAAGGGGAACTAAGTGCAACCAGGAGTTGGAACCAATCAACGGCACAAAATACTCTAACATTCTTAGGGTGCCATTTAGGACAGAGAAGGGAATCCTACGCCGTTGGGTTTCTCGTTTTGACATCTATCCCTACCTTGAATTGTTTACACAGGATGCTACTGCCAAAATCCTCGACCTTATGGAAGGAAAGCCAGATCTTATTATTGGAAACTACACAGATGGGAATTTGGTGGCGTCTCTCATGGCTAATAAACTTGGGATAACTCAGGCAACTATTGCTCATGCTTTGGAGAAGACCAAGTACGAAGATTCAGACATCAACTGGAAGGCGTTAGATCCTAAGTATCACTTCTCATGCCAATTTCTTGCTGACACAATATCCATGAATGCTACAGATTTTGTCATTGCAAGCACATACCAGGAAATTGCTGGAAGCAAAGACAGACCGGGACAATATGAAAGCCACACAGCATTTACGTTTCCGGGGCTCTGCAGAGTTGTTTCAGGCATCAATGTATTTGATCCCAAATTCAACATTGCTGCTCCTGGGGCTGATCAGTCTGTCTATTTCCCCTACGCAGAGAAGCAGAAACGGCTCACTTCATTCCATCCTGCCATTGAAGAACTACTGTTTAGTAAAGAGGATGACAATGAACATATCGGATTTCTAGTGGACAGAAAGAAACCTATCATCTTCTCAATGGCAAGGCTGGACATTGTTAAAAACATTACTGGGTTGGTTGAGTGGTATGGGAAGAACAAGAGGCTGAGAAATTTGGTTAACCTTGTTGTAGTTGGGGGCTTCTTTGACCCTTCGAAATCAAAAGACAGAGAAGAAATTGCAGAAATAAAAAAGATGCATACACTGATAGAGAAGTACGAACTTAGGGGTCAGATCAGATGGATAGCAGCGCAGACTGATAGGAACCGAAATGGAGAGCTCTACCGTTGTATCGCTGACACAAGGGGAGCTTTTGTTCAACCTGCTCTGTATGAAGCTTTCGGCCTGACTGTCATAGAGGCAATGAACTGTGGATTGCCCACCTTTGCAACCAACCAAGGAGGCCCGGCTGAAATCATTGTTGATGGGATCTCTGGTTTCCATATTGATCCCAACAACGGTGACGAAGCAAGCAACAAAATCGCTGATTTCTTCGAGAAGTCTAAGACTGATGCTGCATACTGGGACAGATTTTCAAAAGCAGGCTTGCAGCGCATATACGAATGCTACACTTGGAAGATATATGCAAACAAGGTGTTGAACATGGGGAGTACTTATACGTTCTGGAGGCAGTTGAACAAAGAGCAGAAACAGGCAAAGCAAAGATACATCCAGATGTTCTTCAATCTCCAATATAGAAACTTGGTGAAGGATGTGCCTATCCCAAGTGATGAACCTGAACAACCAGTAGTGCCAAAGCCAACTGCCAAACCAAAGACCACATTAAGGTTGTTTGGAGCCTAA

Protein Analysis

834

Amino Acids

94.48

Weight (kDa)

6.37

Isoelectric Point (pI)

34.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_N PF24861 10 - 123 1e-45 Sucrose synthase N-terminal
SUS_EPBD PF24862 159 - 246 4.6e-26 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 269 - 557 1.8e-170 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 569 - 738 4.6e-31 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 575 - 730 8.7e-13 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 907, 2029
Acc65I GGTACC 1 cut(s) 75
AccB1I GGYRCC 2 cut(s) 75, 1105
AccB7I CCANNNNNTGG 3 cut(s) 196, 908, 1063
AciI CCGC 2 cut(s) 612, 776
AclWI GGATC 4 cut(s) 1367, 1553, 2135, 2141
AcsI RAATTY 6 cut(s) 296, 688, 1005, 1261, 1565, 1810
AcuI CTGAAG 1 cut(s) 608
AcyI GRCGYC 1 cut(s) 1271
AfaI GTAC 6 cut(s) 77, 104, 783, 1340, 1916, 2311
AfeI AGCGCT 1 cut(s) 663
AfiI CCNNNNNNNGG 7 cut(s) 196, 284, 908, 1063, 1076, 2156, 2484
AflIII ACRYGT 1 cut(s) 256
AjnI CCWGG 4 cut(s) 283, 1056, 1455, 1582
AloI GAACNNNNNNTCC 2 cut(s) 1998, 2030
Alw21I GWGCWC 2 cut(s) 654, 1980
Alw26I GTCTC 6 cut(s) 152, 342, 593, 729, 752, 1278
Alw44I GTGCAC 1 cut(s) 650
AlwI GGATC 4 cut(s) 1367, 1553, 2135, 2141
AlwNI CAGNNNCTG 1 cut(s) 908
Aor51HI AGCGCT 1 cut(s) 663
AoxI GGCC 2 cut(s) 2041, 2101
ApaLI GTGCAC 1 cut(s) 650
ApeKI GCWGC 7 cut(s) 11, 14, 629, 1577, 1946, 2213, 2248
ApoI RAATTY 6 cut(s) 296, 688, 1005, 1261, 1565, 1810
Asp718I GGTACC 1 cut(s) 75
AspLEI GCGC 3 cut(s) 664, 1951, 2253
AspS9I GGNCC 2 cut(s) 319, 2102
AsuC2I CCSGG 3 cut(s) 1485, 1521, 2105
AsuHPI GGTGA 3 cut(s) 493, 2171, 2416
AsuII TTCGAA 1 cut(s) 1853
AsuNHI GCTAGC 1 cut(s) 514
AvaII GGWCC 1 cut(s) 319
AxyI CCTNAGG 1 cut(s) 669
BaeGI GKGCMC 1 cut(s) 654
BaeI ACNNNNGTAYC 2 cut(s) 1972, 2005
BanI GGYRCC 2 cut(s) 75, 1105
BanII GRGCYC 2 cut(s) 1527, 1980
BarI GAAGNNNNNNTAC 2 cut(s) 1322, 1354
BbsI GAAGAC 1 cut(s) 1337
Bbv12I GWGCWC 2 cut(s) 654, 1980
BbvI GCAGC 7 cut(s) 23, 26, 641, 1564, 1958, 2200, 2260
BccI CCATC 4 cut(s) 1250, 1653, 1932, 2117
BceAI ACGGC 3 cut(s) 1093, 1122, 1646
BcgI CGANNNNNNTGC 2 cut(s) 2161, 2195
BciT130I CCWGG 4 cut(s) 285, 1058, 1457, 1584
BciVI GTATCC 1 cut(s) 281
BclI TGATCA 3 cut(s) 804, 934, 1591
BcnI CCSGG 3 cut(s) 1485, 1521, 2105
BcoDI GTCTC 6 cut(s) 152, 342, 593, 729, 752, 1278
BfaI CTAG 2 cut(s) 515, 1706
BfmI CTRYAG 5 cut(s) 9, 741, 785, 1428, 1528
BfoI RGCGCY 1 cut(s) 665
BfuAI ACCTGC 2 cut(s) 907, 2029
BfuI GTATCC 1 cut(s) 281
BglII AGATCT 1 cut(s) 1231
BisI GCNGC 7 cut(s) 12, 15, 630, 1578, 1947, 2214, 2249
BlsI GCNGC 7 cut(s) 13, 16, 631, 1579, 1948, 2215, 2250
BmcAI AGTACT 2 cut(s) 783, 2311
Bme1390I CCNGG 7 cut(s) 285, 1058, 1457, 1485, 1521, 1584, 2105
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 319, 2102
BmiI GGNNCC 7 cut(s) 28, 77, 792, 1068, 1107, 1964, 2497
BmrFI CCNGG 7 cut(s) 285, 1058, 1457, 1485, 1521, 1584, 2105
BmrI ACTGGG 3 cut(s) 301, 1779, 2230
BmsI GCATC 6 cut(s) 46, 345, 1180, 1554, 1884, 2200
BmtI GCTAGC 1 cut(s) 518
BmuI ACTGGG 3 cut(s) 301, 1779, 2230
BpiI GAAGAC 1 cut(s) 1337
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BpmI CTGGAG 3 cut(s) 548, 777, 2344
Bpu14I TTCGAA 1 cut(s) 1853
BpuEI CTTGAG 1 cut(s) 431
BpuMI CCSGG 3 cut(s) 1485, 1521, 2105
Bsa29I ATCGAT 1 cut(s) 168
BsaHI GRCGYC 1 cut(s) 1271
BsaI GGTCTC 1 cut(s) 593
BsaJI CCNNGG 3 cut(s) 1520, 1583, 2095
BsaXI ACNNNNNCTCC 2 cut(s) 1998, 2028
Bsc4I CCNNNNNNNGG 7 cut(s) 196, 284, 908, 1063, 1076, 2156, 2484
Bse118I RCCGGY 1 cut(s) 72
Bse1I ACTGG 7 cut(s) 296, 531, 913, 1364, 1774, 2225, 2444
Bse21I CCTNAGG 1 cut(s) 669
Bse3DI GCAATG 3 cut(s) 1440, 1572, 2067
BseBI CCWGG 4 cut(s) 285, 1058, 1457, 1584
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 3 cut(s) 1520, 1583, 2095
BseGI GGATG 6 cut(s) 61, 1195, 1645, 1687, 2367, 2416
BseLI CCNNNNNNNGG 7 cut(s) 196, 284, 908, 1063, 1076, 2156, 2484
BseMI GCAATG 3 cut(s) 1440, 1572, 2067
BseMII CTCAG 5 cut(s) 245, 306, 672, 1316, 1796
BseNI ACTGG 7 cut(s) 296, 531, 913, 1364, 1774, 2225, 2444
BseRI GAGGAG 2 cut(s) 435, 852
BseSI GKGCMC 1 cut(s) 654
BseXI GCAGC 7 cut(s) 23, 26, 641, 1564, 1958, 2200, 2260
Bsh1285I CGRYCG 1 cut(s) 471
BshFI GGCC 2 cut(s) 2043, 2103
BshNI GGYRCC 2 cut(s) 75, 1105
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 471
BsiHKAI GWGCWC 2 cut(s) 654, 1980
BsiSI CCGG 5 cut(s) 53, 73, 1484, 1520, 2105
BslFI GGGAC 2 cut(s) 1500, 2237
BslI CCNNNNNNNGG 7 cut(s) 196, 284, 908, 1063, 1076, 2156, 2484
BsmAI GTCTC 6 cut(s) 152, 342, 593, 729, 752, 1278
BsmBI CGTCTC 1 cut(s) 1278
BsmFI GGGAC 2 cut(s) 1500, 2237
BsmI GAATGC 3 cut(s) 538, 1429, 2267
BsnI GGCC 2 cut(s) 2043, 2103
Bso31I GGTCTC 1 cut(s) 593
Bsp119I TTCGAA 1 cut(s) 1853
Bsp1286I GDGCHC 3 cut(s) 654, 1527, 1980
BspACI CCGC 2 cut(s) 612, 776
BspANI GGCC 2 cut(s) 2043, 2103
BspCNI CTCAG 5 cut(s) 244, 307, 671, 1315, 1797
BspDI ATCGAT 1 cut(s) 168
BspLI GGNNCC 7 cut(s) 28, 77, 792, 1068, 1107, 1964, 2497
BspMAI CTGCAG 3 cut(s) 13, 745, 1532
BspMI ACCTGC 2 cut(s) 907, 2029
BspOI GCTAGC 1 cut(s) 518
BspPI GGATC 4 cut(s) 1367, 1553, 2135, 2141
BspT104I TTCGAA 1 cut(s) 1853
BspT107I GGYRCC 2 cut(s) 75, 1105
BspTNI GGTCTC 1 cut(s) 593
BsrDI GCAATG 3 cut(s) 1440, 1572, 2067
BsrFI RCCGGY 1 cut(s) 72
BsrI ACTGG 7 cut(s) 296, 531, 913, 1364, 1774, 2225, 2444
BssAI RCCGGY 1 cut(s) 72
BssECI CCNNGG 3 cut(s) 1520, 1583, 2095
BssNI GRCGYC 1 cut(s) 1271
BssT1I CCWWGG 1 cut(s) 2095
Bst2UI CCWGG 4 cut(s) 285, 1058, 1457, 1584
Bst4CI ACNGT 8 cut(s) 163, 343, 994, 1668, 1985, 2050, 2069, 2159
Bst6I CTCTTC 1 cut(s) 80
BstACI GRCGYC 1 cut(s) 1271
BstAPI GCANNNNNTGC 1 cut(s) 1313
BstBI TTCGAA 1 cut(s) 1853
BstC8I GCNNGC 6 cut(s) 516, 899, 1447, 2170, 2242, 2246
BstF5I GGATG 6 cut(s) 61, 1195, 1645, 1687, 2367, 2416
BstH2I RGCGCY 1 cut(s) 665
BstHHI GCGC 3 cut(s) 664, 1951, 2253
BstMAI GTCTC 6 cut(s) 152, 342, 593, 729, 752, 1278
BstMCI CGRYCG 1 cut(s) 471
BstMWI GCNNNNNNNGC 5 cut(s) 11, 1313, 1586, 1630, 2250
BstNI CCWGG 4 cut(s) 285, 1058, 1457, 1584
BstNSI RCATGY 2 cut(s) 52, 260
BstSCI CCNGG 7 cut(s) 283, 1056, 1455, 1483, 1519, 1582, 2103
BstSFI CTRYAG 5 cut(s) 9, 741, 785, 1428, 1528
BstSLI GKGCMC 1 cut(s) 654
BstV1I GCAGC 7 cut(s) 23, 26, 641, 1564, 1958, 2200, 2260
BstV2I GAAGAC 1 cut(s) 1337
BstX2I RGATCY 3 cut(s) 1231, 1372, 2127
BstXI CCANNNNNNTGG 1 cut(s) 291
BstYI RGATCY 3 cut(s) 1231, 1372, 2127
Bsu15I ATCGAT 1 cut(s) 168
Bsu36I CCTNAGG 1 cut(s) 669
BsuI GTATCC 1 cut(s) 281
BsuRI GGCC 2 cut(s) 2043, 2103
BsuTUI ATCGAT 1 cut(s) 168
BtsCI GGATG 6 cut(s) 61, 1195, 1645, 1687, 2367, 2416
BtsI GCAGTG 1 cut(s) 738
BtsIMutI CAGTG 5 cut(s) 339, 632, 738, 906, 1901
BveI ACCTGC 2 cut(s) 907, 2029
Cac8I GCNNGC 6 cut(s) 516, 899, 1447, 2170, 2242, 2246
CaiI CAGNNNCTG 1 cut(s) 908
CfoI GCGC 3 cut(s) 664, 1951, 2253
Cfr10I RCCGGY 1 cut(s) 72
Cfr13I GGNCC 2 cut(s) 319, 2102
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 1 cut(s) 1260
Csp6I GTAC 6 cut(s) 76, 103, 782, 1339, 1915, 2310
CviQI GTAC 6 cut(s) 76, 103, 782, 1339, 1915, 2310
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
Ecl136II GAGCTC 1 cut(s) 1978
Eco130I CCWWGG 1 cut(s) 2095
Eco24I GRGCYC 2 cut(s) 1527, 1980
Eco31I GGTCTC 1 cut(s) 593
Eco47I GGWCC 1 cut(s) 319
Eco47III AGCGCT 1 cut(s) 663
Eco53kI GAGCTC 1 cut(s) 1978
Eco57I CTGAAG 1 cut(s) 608
Eco81I CCTNAGG 1 cut(s) 669
EcoICRI GAGCTC 1 cut(s) 1978
EcoO109I RGGNCCY 1 cut(s) 319
EcoRII CCWGG 4 cut(s) 283, 1056, 1455, 1582
EcoT14I CCWWGG 1 cut(s) 2095
EcoT22I ATGCAT 1 cut(s) 1899
EcoT38I GRGCYC 2 cut(s) 1527, 1980
ErhI CCWWGG 1 cut(s) 2095
Esp3I CGTCTC 1 cut(s) 1278
FalI AAGNNNNNCTT 8 cut(s) 77, 109, 948, 980, 1370, 1402, 1993, 2025
FaqI GGGAC 2 cut(s) 1500, 2237
FbaI TGATCA 3 cut(s) 804, 934, 1591
Fnu4HI GCNGC 7 cut(s) 12, 15, 630, 1578, 1947, 2214, 2249
FokI GGATG 6 cut(s) 68, 1202, 1632, 1694, 2354, 2423
FriOI GRGCYC 2 cut(s) 1527, 1980
Fsp4HI GCNGC 7 cut(s) 12, 15, 630, 1578, 1947, 2214, 2249
FspBI CTAG 2 cut(s) 515, 1706
GlaI GCGC 3 cut(s) 663, 1950, 2252
GluI GCNGC 7 cut(s) 12, 15, 630, 1578, 1947, 2214, 2249
GsuI CTGGAG 3 cut(s) 548, 777, 2344
HaeII RGCGCY 1 cut(s) 665
HaeIII GGCC 2 cut(s) 2043, 2103
HapII CCGG 5 cut(s) 53, 73, 1484, 1520, 2105
HgaI GACGC 1 cut(s) 1260
HhaI GCGC 3 cut(s) 664, 1951, 2253
Hin1I GRCGYC 1 cut(s) 1271
Hin6I GCGC 3 cut(s) 662, 1949, 2251
HinP1I GCGC 3 cut(s) 662, 1949, 2251
HincII GTYRAC 1 cut(s) 1819
HindII GTYRAC 1 cut(s) 1819
HindIII AAGCTT 3 cut(s) 620, 945, 2034
HinfI GANTC 5 cut(s) 32, 480, 1023, 1128, 1346
HpaI GTTAAC 1 cut(s) 1819
HpaII CCGG 5 cut(s) 53, 73, 1484, 1520, 2105
HphI GGTGA 3 cut(s) 493, 2171, 2416
Hpy166II GTNNAC 6 cut(s) 501, 652, 925, 1182, 1711, 1819
Hpy188I TCNGA 8 cut(s) 31, 316, 347, 473, 1351, 1700, 1932, 1937
Hpy188III TCNNGA 9 cut(s) 109, 233, 355, 794, 932, 1015, 2194, 2323, 2371
Hpy8I GTNNAC 6 cut(s) 501, 652, 925, 1182, 1711, 1819
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 8 cut(s) 57, 114, 718, 1117, 1214, 1357, 1860, 2401
HpyCH4III ACNGT 8 cut(s) 163, 343, 994, 1668, 1985, 2050, 2069, 2159
HpyCH4IV ACGT 3 cut(s) 105, 1514, 2318
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 1313, 1586, 1630, 2250
HpySE526I ACGT 3 cut(s) 105, 1514, 2318
Hsp92I GRCGYC 1 cut(s) 1271
HspAI GCGC 3 cut(s) 662, 1949, 2251
KpnI GGTACC 1 cut(s) 79
Ksp22I TGATCA 3 cut(s) 804, 934, 1591
KspAI GTTAAC 1 cut(s) 1819
LmnI GCTCC 6 cut(s) 32, 138, 796, 1585, 2006, 2495
Lsp1109I GCAGC 7 cut(s) 23, 26, 641, 1564, 1958, 2200, 2260
LweI GCATC 6 cut(s) 46, 345, 1180, 1554, 1884, 2200
MaeI CTAG 2 cut(s) 515, 1706
MaeII ACGT 3 cut(s) 105, 1514, 2318
MaeIII GTNAC 4 cut(s) 490, 640, 1015, 2159
MfeI CAATTG 2 cut(s) 327, 429
MflI RGATCY 3 cut(s) 1231, 1372, 2127
MhlI GDGCHC 3 cut(s) 654, 1527, 1980
MlyI GAGTC 2 cut(s) 41, 1017
MmeI TCCRAC 1 cut(s) 1044
Mph1103I ATGCAT 1 cut(s) 1899
MseI TTAA 7 cut(s) 303, 452, 705, 975, 1758, 1818, 2483
MslI CAYNNNNRTG 1 cut(s) 2372
MspI CCGG 5 cut(s) 53, 73, 1484, 1520, 2105
MspR9I CCNGG 7 cut(s) 285, 1058, 1457, 1485, 1521, 1584, 2105
MunI CAATTG 2 cut(s) 327, 429
Mva1269I GAATGC 3 cut(s) 538, 1429, 2267
MvaI CCWGG 4 cut(s) 285, 1058, 1457, 1584
MwoI GCNNNNNNNGC 5 cut(s) 11, 1313, 1586, 1630, 2250
NciI CCSGG 3 cut(s) 1485, 1521, 2105
NheI GCTAGC 1 cut(s) 514
NlaIV GGNNCC 7 cut(s) 28, 77, 792, 1068, 1107, 1964, 2497
NmuCI GTSAC 2 cut(s) 1015, 2159
NsiI ATGCAT 1 cut(s) 1899
NspI RCATGY 2 cut(s) 52, 260
NspV TTCGAA 1 cut(s) 1853
PciI ACATGT 1 cut(s) 256
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 3 cut(s) 538, 1429, 2267
PfeI GAWTC 3 cut(s) 480, 1128, 1346
PflMI CCANNNNNTGG 3 cut(s) 196, 908, 1063
PfoI TCCNGGA 1 cut(s) 283
PkrI GCNGC 7 cut(s) 13, 16, 631, 1579, 1948, 2215, 2250
Ple19I CGATCG 1 cut(s) 471
PleI GAGTC 2 cut(s) 40, 1017
PpsI GAGTC 2 cut(s) 40, 1017
PpuMI RGGWCCY 1 cut(s) 319
PscI ACATGT 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 1980
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 4 cut(s) 283, 1056, 1455, 1582
PspGI CCWGG 4 cut(s) 283, 1056, 1455, 1582
PspN4I GGNNCC 7 cut(s) 28, 77, 792, 1068, 1107, 1964, 2497
PspPI GGNCC 2 cut(s) 319, 2102
PspPPI RGGWCCY 1 cut(s) 319
PstI CTGCAG 3 cut(s) 13, 745, 1532
PstNI CAGNNNCTG 1 cut(s) 908
PsuI RGATCY 3 cut(s) 1231, 1372, 2127
PvuI CGATCG 1 cut(s) 471
RsaI GTAC 6 cut(s) 77, 104, 783, 1340, 1916, 2311
RsaNI GTAC 6 cut(s) 76, 103, 782, 1339, 1915, 2310
RseI CAYNNNNRTG 1 cut(s) 2372
SacI GAGCTC 1 cut(s) 1980
SaqAI TTAA 7 cut(s) 303, 452, 705, 975, 1758, 1818, 2483
SatI GCNGC 7 cut(s) 12, 15, 630, 1578, 1947, 2214, 2249
Sau96I GGNCC 2 cut(s) 319, 2102
ScaI AGTACT 2 cut(s) 783, 2311
SchI GAGTC 2 cut(s) 41, 1017
ScrFI CCNGG 7 cut(s) 285, 1058, 1457, 1485, 1521, 1584, 2105
SduI GDGCHC 3 cut(s) 654, 1527, 1980
SfaNI GCATC 6 cut(s) 46, 345, 1180, 1554, 1884, 2200
SfcI CTRYAG 5 cut(s) 9, 741, 785, 1428, 1528
SfuI TTCGAA 1 cut(s) 1853
SinI GGWCC 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 2372
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
SsiI CCGC 2 cut(s) 612, 776
SspI AATATT 2 cut(s) 840, 855
SspMI CTAG 2 cut(s) 515, 1706
SstI GAGCTC 1 cut(s) 1980
StyD4I CCNGG 7 cut(s) 283, 1056, 1455, 1483, 1519, 1582, 2103
StyI CCWWGG 1 cut(s) 2095
TaaI ACNGT 8 cut(s) 163, 343, 994, 1668, 1985, 2050, 2069, 2159
TaiI ACGT 3 cut(s) 108, 1517, 2321
TaqI TCGA 6 cut(s) 108, 168, 467, 1209, 1853, 2193
TatI WGTACW 2 cut(s) 781, 2309
TfiI GAWTC 3 cut(s) 480, 1128, 1346
Tru1I TTAA 7 cut(s) 303, 452, 705, 975, 1758, 1818, 2483
Tru9I TTAA 7 cut(s) 303, 452, 705, 975, 1758, 1818, 2483
TscAI CASTG 5 cut(s) 346, 639, 745, 913, 1908
TseFI GTSAC 2 cut(s) 1015, 2159
TseI GCWGC 7 cut(s) 11, 14, 629, 1577, 1946, 2213, 2248
Tsp45I GTSAC 2 cut(s) 1015, 2159
TspDTI ATGAA 9 cut(s) 98, 414, 1436, 1509, 1629, 1704, 2046, 2078, 2445
TspRI CASTG 5 cut(s) 346, 639, 745, 913, 1908
Van91I CCANNNNNTGG 3 cut(s) 196, 908, 1063
VneI GTGCAC 1 cut(s) 650
VpaK11BI GGWCC 1 cut(s) 319
XapI RAATTY 6 cut(s) 296, 688, 1005, 1261, 1565, 1810
XceI RCATGY 2 cut(s) 52, 260
XcmI CCANNNNNNNNNTGG 1 cut(s) 1463
XspI CTAG 2 cut(s) 515, 1706
ZrmI AGTACT 2 cut(s) 783, 2311
Zsp2I ATGCAT 1 cut(s) 1899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.