MD17G1287000.v1.1

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004012
Physical Location & Seq
Reverse (-)
34656468 .. 34660897
4430 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1287000.v1.1.491

Sequence Viewer

Length: 2532 bp
ATGGCTTCTGCAGCGGCCATCAAACGCTCCGATTCCATAGCTGATACCATGCCGGACGCCCTGAGGCAGAGCCGATACCACATGAAGCGGTGCTTTGCAAAGTACATCGAGCAAGGAAGAAGGATCATGAAGCTTCACCATTTGATGAGTGAAATGGAGACAGTCATCGATGACAAGGCTGAAAGAACCCAAGTTTTGGGGGGTGTTCTTGGCTATATATTGTGTTCAACTCAGGAAGCTGTTGTTGTTCCTCCACATGTTGTCTTTTCAATAAGACTAAATCCAGGATATTGGGAATTCGTTAAGGTCAGCTCTGAGGATCTCTCAGTTGAGGCTATCACTGTCCGAGACTTCTTGAAATACAAAGAAGCCTTATATGATGAGAAATGGTCAAATGACGAACATGTATTGGAGGTGGATTTTAGAGCAATTGATTTCTCTACTCCTCACTTGACTCTATCTTCCTCGATCGGAAATGGAATCGAATATGTTACCAAGTTCACCACTTCAAAGCTAGCTGGAAAACTGGAGAATGCACAACCCCTTGTGGATTATTTACTTTCACTAAACCATCAAGGAGAACAACTTATTCTAAATGAGACCCTCAACACAGCTTCGAAGCTTCAGGCGGCTCTGATTGTAACAGAAGTGTACCTCTCAACACTTCCTAAGGACACACCATTCCAAAATTTTGAGCTAAGGTTTAAGGAGTGGGGCTTTGAGAAGGGGTGGGGAGACACTGCAGAAAGAACAAAGGAGACAATGAAAATACTTTCAGAAGTACTTCAAGCTCCAGACCCGTTGAACATGGATAGGTTTTTCAGTAGGCTTCCCATAATATTCAATGTTGTAATATTCTCTCCCCATGGCTACTTTGGCCAAGCAGATGTTCTTGGCTTACCAGACACAGGCGGGCAGGTAGTTTACATTCTGGATCAAGTGAAAGCTATGGAGGAAGAATTGACTCTGAGAATTAAGCAACAAGGACTTACTGTGAAGCCTCAAATTCTTGTGGTGACAAGACTCATACCCGAAGCAAGGGGAACTAAGTGCAACCAGGAGTTGGAACCAATCTATGGCACCAAGTACTCTAACATCCTTAGGGTGCCATTTAGGACAGAAAAGGGTATCCTGCGCCGTTGGGTTTCTCGTTTTGACATCTATCCCTATCTTGAGTTGTTTACACAGGATGCTACTGCCAAAATCCTCGACCTGATGGAAGGAAAGCCGGATCTTATTATTGGAAACTACACAGATGGCAATTTGGTGGCGTCTCTCATGGCTAATAAACTTGGGATAACTCAGGCAACTATTGCTCATGCTTTGGAGAAGACCAAGTACGAAAATTCAGACATCAACTGGAAGGAGTTAGATCCCAAGTATCACTTCTCATGCCAATTTCTTGCTGACACAATCTCAATGAATGCTACAGATTTTGTCATTGCAAGCACATACCAGGAAATTGCAGGAAGCAAAGACAGACCAGGACAATACGAAAGTCATACTGCATTTACACTCCCGGGGCTCTGTAGAGTTGTTTCTGGCATCAATGTATTTGACCCCAAATTCAACATTGCCGCTCCTGGGGCAGATCAGTCTGTATATTTCCCCTACTCAGACAAGCAGAAACGGCTCACTTCATTCTATCCTGCCATTGAAGAACTACTGTTTAGTAAAGAGGATAACAGTGAACATCTTGGATTTCTAGTAGACCGGAAGAAACCTATCATCTTCTCAATGGCAAGGCTGGATATTGTTAAAAACATTACCGGGTTGGTTGAGTGGTACGGGAAGAACAAGAGGCTGAGAAATTTGGTTAATCTTGTGGTAGTTGGTGGATTCTTTGACCCTTCAAAGTCAAAAGATAGAGAAGAAATCGCTGAAATAAAAAAGATGCATACACTGATAGAGAAGTACCAACTCAGGGGTCAGATCAGATGGATAGCAGCACAGACTGATAGGAACCGAAATGGAGAGCTCTACCGTTGCATTGCTGACACAAGGGGAGCTTTTGTGCAACCTGCCCTGTATGAAGCTTTCGGTCTGACTGTCATTGAGGCAATGAACTGTGGATTGCCCACCTTTGCAACCAACCAAGGAGGCCCGGCTGAAATCATCATTGATGGGGTCTCTGGTTTCCATATTGATCCTAACAACGGTGATGAAGCAAGCAACAAAATCGCTGATTTCTTCGAGAACTCTAAGACTGATGCAGCATACTGGGACAGATTTTCCAAAGCAGGCTTGCAGCGCATATACGAATGCTACACTTGGAAGATATATGCAAACAAGGTGTTGAACATGGGGAGCACTTATACTTTCTGGAGGCAGTTGAACAAAGAGCAGAAACAGGCAAAGCAAAGATACATCCAGATGTTCTTTAATCTCCAATATAAGAACTTGGTGAAGAATGTACCTGTCTCAAGTGATGAACCTGAGCAACCAGCTGCGCCAAAGGCAACTTCCAAACCACAGACCACATTAAGCTCAAGGCGCTCACAGTCTCGATTGCAAAGGTTGTTTGGAAGTTAA

Protein Analysis

844

Amino Acids

95.85

Weight (kDa)

7.21

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_N PF24861 9 - 123 2.4e-46 Sucrose synthase N-terminal
SUS_EPBD PF24862 159 - 246 6e-27 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 269 - 557 2.5e-169 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 571 - 738 1.1e-31 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 575 - 732 1.7e-13 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 907, 2029
AccB1I GGYRCC 2 cut(s) 1079, 1105
AccB7I CCANNNNNTGG 3 cut(s) 196, 1063, 1076
AccBSI CCGCTC 1 cut(s) 1580
AccI GTMKAC 1 cut(s) 1710
AciI CCGC 5 cut(s) 14, 88, 629, 912, 1578
AclWI GGATC 6 cut(s) 131, 327, 942, 1239, 1367, 2141
AcoI YGGCCR 2 cut(s) 15, 877
AcsI RAATTY 6 cut(s) 296, 688, 1005, 1345, 1565, 1810
AcuI CTGAAG 1 cut(s) 608
AcyI GRCGYC 2 cut(s) 57, 1271
AfaI GTAC 8 cut(s) 104, 653, 783, 1088, 1340, 1787, 1916, 2415
AfiI CCNNNNNNNGG 8 cut(s) 196, 908, 1038, 1063, 1076, 1584, 1924, 2156
AflIII ACRYGT 2 cut(s) 256, 403
AjnI CCWGG 5 cut(s) 283, 1056, 1455, 1483, 1582
AjuI GAANNNNNNNTTGG 6 cut(s) 873, 905, 1370, 1402, 2446, 2478
Alw21I GWGCWC 2 cut(s) 1980, 2312
Alw26I GTCTC 9 cut(s) 152, 342, 593, 729, 752, 1278, 2134, 2425, 2508
AlwI GGATC 6 cut(s) 131, 327, 942, 1239, 1367, 2141
Ama87I CYCGRG 1 cut(s) 1519
AoxI GGCC 3 cut(s) 15, 877, 2101
ApeKI GCWGC 5 cut(s) 11, 1946, 2213, 2248, 2447
ApoI RAATTY 6 cut(s) 296, 688, 1005, 1345, 1565, 1810
Asp700I GAANNNNTTC 1 cut(s) 783
AspLEI GCGC 4 cut(s) 1137, 2253, 2452, 2496
AspS9I GGNCC 1 cut(s) 2102
AsuC2I CCSGG 4 cut(s) 1520, 1521, 1771, 2105
AsuHPI GGTGA 5 cut(s) 128, 493, 1027, 2171, 2416
AsuII TTCGAA 1 cut(s) 617
AsuNHI GCTAGC 1 cut(s) 514
AvaI CYCGRG 1 cut(s) 1519
AxyI CCTNAGG 3 cut(s) 62, 669, 1100
BalI TGGCCA 1 cut(s) 879
BanI GGYRCC 2 cut(s) 1079, 1105
BanII GRGCYC 2 cut(s) 1527, 1980
BarI GAAGNNNNNNTAC 2 cut(s) 1322, 1354
BbsI GAAGAC 1 cut(s) 1337
Bbv12I GWGCWC 2 cut(s) 1980, 2312
BbvI GCAGC 5 cut(s) 23, 1958, 2225, 2260, 2434
BccI CCATC 6 cut(s) 26, 579, 1210, 1250, 1932, 2117
BceAI ACGGC 2 cut(s) 1122, 1646
BcgI CGANNNNNNTGC 2 cut(s) 2161, 2195
BciT130I CCWGG 5 cut(s) 285, 1058, 1457, 1485, 1584
BciVI GTATCC 1 cut(s) 1139
BcnI CCSGG 4 cut(s) 1520, 1521, 1771, 2105
BcoDI GTCTC 9 cut(s) 152, 342, 593, 729, 752, 1278, 2134, 2425, 2508
BfaI CTAG 2 cut(s) 515, 1706
BfmI CTRYAG 4 cut(s) 9, 741, 1428, 1528
BfoI RGCGCY 1 cut(s) 2497
BfuAI ACCTGC 2 cut(s) 907, 2029
BfuI GTATCC 1 cut(s) 1139
BisI GCNGC 8 cut(s) 12, 15, 630, 1578, 1947, 2214, 2249, 2448
BlsI GCNGC 8 cut(s) 13, 16, 631, 1579, 1948, 2215, 2250, 2449
BmcAI AGTACT 2 cut(s) 783, 1088
Bme1390I CCNGG 9 cut(s) 285, 1058, 1457, 1485, 1520, 1521, 1584, 1771, 2105
BmeT110I CYCGRG 1 cut(s) 1519
BmgT120I GGNCC 1 cut(s) 2102
BmiI GGNNCC 4 cut(s) 1068, 1081, 1107, 1964
BmrFI CCNGG 9 cut(s) 285, 1058, 1457, 1485, 1520, 1521, 1584, 1771, 2105
BmrI ACTGGG 1 cut(s) 2230
BmsI GCATC 4 cut(s) 1180, 1554, 1884, 2200
BmtI GCTAGC 1 cut(s) 518
BmuI ACTGGG 1 cut(s) 2230
BpiI GAAGAC 1 cut(s) 1337
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BpmI CTGGAG 3 cut(s) 548, 777, 2344
Bpu10I CCTNAGC 2 cut(s) 698, 2436
Bpu14I TTCGAA 1 cut(s) 617
BpuEI CTTGAG 3 cut(s) 1193, 2407, 2473
BpuMI CCSGG 4 cut(s) 1520, 1521, 1771, 2105
Bsa29I ATCGAT 1 cut(s) 168
BsaHI GRCGYC 2 cut(s) 57, 1271
BsaI GGTCTC 2 cut(s) 593, 2134
BsaJI CCNNGG 5 cut(s) 865, 1519, 1520, 1583, 2095
BsaWI WCCGGW 1 cut(s) 1713
BsaXI ACNNNNNCTCC 2 cut(s) 1998, 2028
Bsc4I CCNNNNNNNGG 8 cut(s) 196, 908, 1038, 1063, 1076, 1584, 1924, 2156
Bse1I ACTGG 3 cut(s) 531, 1364, 2225
Bse21I CCTNAGG 3 cut(s) 62, 669, 1100
Bse3DI GCAATG 4 cut(s) 1440, 1572, 1989, 2067
BseBI CCWGG 5 cut(s) 285, 1058, 1457, 1485, 1584
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 5 cut(s) 865, 1519, 1520, 1583, 2095
BseGI GGATG 3 cut(s) 1095, 1195, 2367
BseLI CCNNNNNNNGG 8 cut(s) 196, 908, 1038, 1063, 1076, 1584, 1924, 2156
BseMI GCAATG 4 cut(s) 1440, 1572, 1989, 2067
BseNI ACTGG 3 cut(s) 531, 1364, 2225
BseRI GAGGAG 1 cut(s) 435
BseXI GCAGC 5 cut(s) 23, 1958, 2225, 2260, 2434
Bsh1285I CGRYCG 1 cut(s) 471
BshFI GGCC 3 cut(s) 17, 879, 2103
BshNI GGYRCC 2 cut(s) 1079, 1105
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 471
BsiHKAI GWGCWC 2 cut(s) 1980, 2312
BsiHKCI CYCGRG 1 cut(s) 1519
BsiSI CCGG 6 cut(s) 53, 1229, 1520, 1714, 1770, 2105
BslFI GGGAC 1 cut(s) 2237
BslI CCNNNNNNNGG 8 cut(s) 196, 908, 1038, 1063, 1076, 1584, 1924, 2156
BsmAI GTCTC 9 cut(s) 152, 342, 593, 729, 752, 1278, 2134, 2425, 2508
BsmBI CGTCTC 1 cut(s) 1278
BsmFI GGGAC 1 cut(s) 2237
BsmI GAATGC 3 cut(s) 538, 1429, 2267
BsnI GGCC 3 cut(s) 17, 879, 2103
Bso31I GGTCTC 2 cut(s) 593, 2134
BsoBI CYCGRG 1 cut(s) 1519
Bsp119I TTCGAA 1 cut(s) 617
Bsp1286I GDGCHC 3 cut(s) 1527, 1980, 2312
Bsp143I GATC 9 cut(s) 123, 319, 468, 934, 1231, 1372, 1591, 1932, 2146
Bsp19I CCATGG 1 cut(s) 865
BspACI CCGC 5 cut(s) 14, 88, 629, 912, 1578
BspANI GGCC 3 cut(s) 17, 879, 2103
BspDI ATCGAT 1 cut(s) 168
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 4 cut(s) 1068, 1081, 1107, 1964
BspMAI CTGCAG 2 cut(s) 13, 745
BspMI ACCTGC 2 cut(s) 907, 2029
BspOI GCTAGC 1 cut(s) 518
BspPI GGATC 6 cut(s) 131, 327, 942, 1239, 1367, 2141
BspT104I TTCGAA 1 cut(s) 617
BspT107I GGYRCC 2 cut(s) 1079, 1105
BspTNI GGTCTC 2 cut(s) 593, 2134
BsrBI CCGCTC 1 cut(s) 1580
BsrDI GCAATG 4 cut(s) 1440, 1572, 1989, 2067
BsrI ACTGG 3 cut(s) 531, 1364, 2225
BssECI CCNNGG 5 cut(s) 865, 1519, 1520, 1583, 2095
BssMI GATC 9 cut(s) 123, 319, 468, 934, 1231, 1372, 1591, 1932, 2146
BssNI GRCGYC 2 cut(s) 57, 1271
BssT1I CCWWGG 2 cut(s) 865, 2095
Bst2UI CCWGG 5 cut(s) 285, 1058, 1457, 1485, 1584
BstACI GRCGYC 2 cut(s) 57, 1271
BstAPI GCANNNNNTGC 1 cut(s) 1313
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 6 cut(s) 516, 914, 1447, 2170, 2242, 2246
BstDSI CCRYGG 1 cut(s) 865
BstF5I GGATG 3 cut(s) 1095, 1195, 2367
BstH2I RGCGCY 1 cut(s) 2497
BstHHI GCGC 4 cut(s) 1137, 2253, 2452, 2496
BstKTI GATC 9 cut(s) 126, 322, 471, 937, 1234, 1375, 1594, 1935, 2149
BstMAI GTCTC 9 cut(s) 152, 342, 593, 729, 752, 1278, 2134, 2425, 2508
BstMBI GATC 9 cut(s) 123, 319, 468, 934, 1231, 1372, 1591, 1932, 2146
BstMCI CGRYCG 1 cut(s) 471
BstMWI GCNNNNNNNGC 8 cut(s) 11, 876, 1313, 1586, 1630, 2250, 2456, 2493
BstNI CCWGG 5 cut(s) 285, 1058, 1457, 1485, 1584
BstNSI RCATGY 2 cut(s) 260, 407
BstSCI CCNGG 9 cut(s) 283, 1056, 1455, 1483, 1518, 1519, 1582, 1769, 2103
BstSFI CTRYAG 4 cut(s) 9, 741, 1428, 1528
BstV1I GCAGC 5 cut(s) 23, 1958, 2225, 2260, 2434
BstV2I GAAGAC 1 cut(s) 1337
BstX2I RGATCY 3 cut(s) 319, 1231, 1372
BstXI CCANNNNNNTGG 1 cut(s) 291
BstYI RGATCY 3 cut(s) 319, 1231, 1372
Bsu15I ATCGAT 1 cut(s) 168
Bsu36I CCTNAGG 3 cut(s) 62, 669, 1100
BsuI GTATCC 1 cut(s) 1139
BsuRI GGCC 3 cut(s) 17, 879, 2103
BsuTUI ATCGAT 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 865
BtsCI GGATG 3 cut(s) 1095, 1195, 2367
BtsI GCAGTG 1 cut(s) 738
BtsIMutI CAGTG 4 cut(s) 339, 738, 1693, 1901
BveI ACCTGC 2 cut(s) 907, 2029
Cac8I GCNNGC 6 cut(s) 516, 914, 1447, 2170, 2242, 2246
CciI TCATGA 1 cut(s) 126
CfoI GCGC 4 cut(s) 1137, 2253, 2452, 2496
Cfr13I GGNCC 1 cut(s) 2102
Cfr9I CCCGGG 1 cut(s) 1519
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 2 cut(s) 65, 1260
Csp6I GTAC 8 cut(s) 103, 652, 782, 1087, 1339, 1786, 1915, 2414
CviQI GTAC 8 cut(s) 103, 652, 782, 1087, 1339, 1786, 1915, 2414
DpnI GATC 9 cut(s) 125, 321, 470, 936, 1233, 1374, 1593, 1934, 2148
DpnII GATC 9 cut(s) 123, 319, 468, 934, 1231, 1372, 1591, 1932, 2146
EaeI YGGCCR 2 cut(s) 15, 877
Ecl136II GAGCTC 1 cut(s) 1978
Eco130I CCWWGG 2 cut(s) 865, 2095
Eco24I GRGCYC 2 cut(s) 1527, 1980
Eco31I GGTCTC 2 cut(s) 593, 2134
Eco53kI GAGCTC 1 cut(s) 1978
Eco57I CTGAAG 1 cut(s) 608
Eco81I CCTNAGG 3 cut(s) 62, 669, 1100
Eco88I CYCGRG 1 cut(s) 1519
EcoICRI GAGCTC 1 cut(s) 1978
EcoRI GAATTC 1 cut(s) 296
EcoRII CCWGG 5 cut(s) 283, 1056, 1455, 1483, 1582
EcoT14I CCWWGG 2 cut(s) 865, 2095
EcoT22I ATGCAT 1 cut(s) 1899
EcoT38I GRGCYC 2 cut(s) 1527, 1980
ErhI CCWWGG 2 cut(s) 865, 2095
Esp3I CGTCTC 1 cut(s) 1278
FalI AAGNNNNNCTT 6 cut(s) 77, 109, 1370, 1402, 1993, 2025
FaqI GGGAC 1 cut(s) 2237
FauI CCCGC 1 cut(s) 905
FblI GTMKAC 1 cut(s) 1710
Fnu4HI GCNGC 8 cut(s) 12, 15, 630, 1578, 1947, 2214, 2249, 2448
FokI GGATG 3 cut(s) 1082, 1202, 2354
FriOI GRGCYC 2 cut(s) 1527, 1980
Fsp4HI GCNGC 8 cut(s) 12, 15, 630, 1578, 1947, 2214, 2249, 2448
FspBI CTAG 2 cut(s) 515, 1706
GlaI GCGC 4 cut(s) 1136, 2252, 2451, 2495
GluI GCNGC 8 cut(s) 12, 15, 630, 1578, 1947, 2214, 2249, 2448
GsuI CTGGAG 3 cut(s) 548, 777, 2344
HaeII RGCGCY 1 cut(s) 2497
HaeIII GGCC 3 cut(s) 17, 879, 2103
HapII CCGG 6 cut(s) 53, 1229, 1520, 1714, 1770, 2105
HgaI GACGC 2 cut(s) 65, 1260
HhaI GCGC 4 cut(s) 1137, 2253, 2452, 2496
Hin1I GRCGYC 2 cut(s) 57, 1271
Hin6I GCGC 4 cut(s) 1135, 2251, 2450, 2494
HinP1I GCGC 4 cut(s) 1135, 2251, 2450, 2494
HindIII AAGCTT 3 cut(s) 131, 620, 2034
HinfI GANTC 6 cut(s) 32, 454, 480, 964, 1023, 1839
HpaII CCGG 6 cut(s) 53, 1229, 1520, 1714, 1770, 2105
HphI GGTGA 5 cut(s) 128, 493, 1027, 2171, 2416
Hpy166II GTNNAC 6 cut(s) 501, 652, 925, 1182, 1691, 1711
Hpy8I GTNNAC 6 cut(s) 501, 652, 925, 1182, 1691, 1711
HpyAV CCTTC 5 cut(s) 114, 718, 1214, 1357, 1860
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 876, 1313, 1586, 1630, 2250, 2456, 2493
Hsp92I GRCGYC 2 cut(s) 57, 1271
HspAI GCGC 4 cut(s) 1135, 2251, 2450, 2494
Kzo9I GATC 9 cut(s) 123, 319, 468, 934, 1231, 1372, 1591, 1932, 2146
LmnI GCTCC 5 cut(s) 32, 796, 1585, 2006, 2307
Lsp1109I GCAGC 5 cut(s) 23, 1958, 2225, 2260, 2434
LweI GCATC 4 cut(s) 1180, 1554, 1884, 2200
MaeI CTAG 2 cut(s) 515, 1706
MaeIII GTNAC 3 cut(s) 490, 640, 1015
MalI GATC 9 cut(s) 125, 321, 470, 936, 1233, 1374, 1593, 1934, 2148
MbiI CCGCTC 1 cut(s) 1580
MboI GATC 9 cut(s) 123, 319, 468, 934, 1231, 1372, 1591, 1932, 2146
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 3 cut(s) 319, 1231, 1372
MhlI GDGCHC 3 cut(s) 1527, 1980, 2312
MlsI TGGCCA 1 cut(s) 879
MluNI TGGCCA 1 cut(s) 879
MlyI GAGTC 3 cut(s) 448, 958, 1017
MmeI TCCRAC 1 cut(s) 1044
Mox20I TGGCCA 1 cut(s) 879
Mph1103I ATGCAT 1 cut(s) 1899
MroXI GAANNNNTTC 1 cut(s) 783
MscI TGGCCA 1 cut(s) 879
MseI TTAA 8 cut(s) 303, 705, 975, 1758, 1818, 2382, 2483, 2530
MslI CAYNNNNRTG 1 cut(s) 2372
Msp20I TGGCCA 1 cut(s) 879
MspA1I CMGCKG 2 cut(s) 14, 2447
MspI CCGG 6 cut(s) 53, 1229, 1520, 1714, 1770, 2105
MspR9I CCNGG 9 cut(s) 285, 1058, 1457, 1485, 1520, 1521, 1584, 1771, 2105
MunI CAATTG 1 cut(s) 429
Mva1269I GAATGC 3 cut(s) 538, 1429, 2267
MvaI CCWGG 5 cut(s) 285, 1058, 1457, 1485, 1584
MwoI GCNNNNNNNGC 8 cut(s) 11, 876, 1313, 1586, 1630, 2250, 2456, 2493
NciI CCSGG 4 cut(s) 1520, 1521, 1771, 2105
NcoI CCATGG 1 cut(s) 865
NdeII GATC 9 cut(s) 123, 319, 468, 934, 1231, 1372, 1591, 1932, 2146
NheI GCTAGC 1 cut(s) 514
NlaIV GGNNCC 4 cut(s) 1068, 1081, 1107, 1964
NmuCI GTSAC 1 cut(s) 1015
NsiI ATGCAT 1 cut(s) 1899
NspI RCATGY 2 cut(s) 260, 407
NspV TTCGAA 1 cut(s) 617
PagI TCATGA 1 cut(s) 126
PciI ACATGT 2 cut(s) 256, 403
PctI GAATGC 3 cut(s) 538, 1429, 2267
PdmI GAANNNNTTC 1 cut(s) 783
PfeI GAWTC 3 cut(s) 32, 480, 1839
PflMI CCANNNNNTGG 3 cut(s) 196, 1063, 1076
PfoI TCCNGGA 1 cut(s) 283
PkrI GCNGC 8 cut(s) 13, 16, 631, 1579, 1948, 2215, 2250, 2449
Ple19I CGATCG 1 cut(s) 471
PleI GAGTC 3 cut(s) 448, 958, 1017
PpsI GAGTC 3 cut(s) 448, 958, 1017
PscI ACATGT 2 cut(s) 256, 403
Psp124BI GAGCTC 1 cut(s) 1980
Psp6I CCWGG 5 cut(s) 283, 1056, 1455, 1483, 1582
PspGI CCWGG 5 cut(s) 283, 1056, 1455, 1483, 1582
PspN4I GGNNCC 4 cut(s) 1068, 1081, 1107, 1964
PspPI GGNCC 1 cut(s) 2102
PstI CTGCAG 2 cut(s) 13, 745
PsuI RGATCY 3 cut(s) 319, 1231, 1372
PvuI CGATCG 1 cut(s) 471
PvuII CAGCTG 1 cut(s) 2447
RsaI GTAC 8 cut(s) 104, 653, 783, 1088, 1340, 1787, 1916, 2415
RsaNI GTAC 8 cut(s) 103, 652, 782, 1087, 1339, 1786, 1915, 2414
RseI CAYNNNNRTG 1 cut(s) 2372
SacI GAGCTC 1 cut(s) 1980
SaqAI TTAA 8 cut(s) 303, 705, 975, 1758, 1818, 2382, 2483, 2530
SatI GCNGC 8 cut(s) 12, 15, 630, 1578, 1947, 2214, 2249, 2448
Sau3AI GATC 9 cut(s) 123, 319, 468, 934, 1231, 1372, 1591, 1932, 2146
Sau96I GGNCC 1 cut(s) 2102
ScaI AGTACT 2 cut(s) 783, 1088
SchI GAGTC 3 cut(s) 448, 958, 1017
ScrFI CCNGG 9 cut(s) 285, 1058, 1457, 1485, 1520, 1521, 1584, 1771, 2105
SduI GDGCHC 3 cut(s) 1527, 1980, 2312
SfaNI GCATC 4 cut(s) 1180, 1554, 1884, 2200
SfcI CTRYAG 4 cut(s) 9, 741, 1428, 1528
SfuI TTCGAA 1 cut(s) 617
SmaI CCCGGG 1 cut(s) 1521
SmiMI CAYNNNNRTG 1 cut(s) 2372
SmlI CTYRAG 3 cut(s) 1172, 2422, 2488
SmoI CTYRAG 3 cut(s) 1172, 2422, 2488
SsiI CCGC 5 cut(s) 14, 88, 629, 912, 1578
SspI AATATT 2 cut(s) 840, 855
SspMI CTAG 2 cut(s) 515, 1706
SstI GAGCTC 1 cut(s) 1980
StyD4I CCNGG 9 cut(s) 283, 1056, 1455, 1483, 1518, 1519, 1582, 1769, 2103
StyI CCWWGG 2 cut(s) 865, 2095
TaqI TCGA 8 cut(s) 108, 168, 467, 483, 617, 1209, 2193, 2506
TaqII GACCGA 1 cut(s) 2030
TatI WGTACW 3 cut(s) 102, 781, 1086
TauI GCSGC 3 cut(s) 17, 632, 1580
TfiI GAWTC 3 cut(s) 32, 480, 1839
Tru1I TTAA 8 cut(s) 303, 705, 975, 1758, 1818, 2382, 2483, 2530
Tru9I TTAA 8 cut(s) 303, 705, 975, 1758, 1818, 2382, 2483, 2530
TscAI CASTG 4 cut(s) 346, 745, 1693, 1908
TseFI GTSAC 1 cut(s) 1015
TseI GCWGC 5 cut(s) 11, 1946, 2213, 2248, 2447
Tsp45I GTSAC 1 cut(s) 1015
TspDTI ATGAA 9 cut(s) 98, 143, 779, 1436, 1629, 2046, 2078, 2178, 2445
TspMI CCCGGG 1 cut(s) 1519
TspRI CASTG 4 cut(s) 346, 745, 1693, 1908
Van91I CCANNNNNTGG 3 cut(s) 196, 1063, 1076
XapI RAATTY 6 cut(s) 296, 688, 1005, 1345, 1565, 1810
XceI RCATGY 2 cut(s) 260, 407
XcmI CCANNNNNNNNNTGG 1 cut(s) 872
XmaI CCCGGG 1 cut(s) 1519
XmiI GTMKAC 1 cut(s) 1710
XmnI GAANNNNTTC 1 cut(s) 783
XspI CTAG 2 cut(s) 515, 1706
ZrmI AGTACT 2 cut(s) 783, 1088
Zsp2I ATGCAT 1 cut(s) 1899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.