Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004012

Physical Location & Seq

Genomic Coordinates Reverse (-)

34650839 .. 34654753

Gene Structure 3915 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1286900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2034 bp

ATGGCTTCTGCAGCGGCCGTCAAACGCTCCGATTCCATAGCTGAAACCATGCCGGACGCCCTGAGGCAGAGCCGTTACCACATGAAGCGGTGCTTCGCTATGTACATCGAGAAAGGAAGAAGGATAATGAAGCTTCACCATTTAATGAGTGAAATGGAGACAGTCATAGATGACAAGGCTGAAAGAACCCAAGTTTTGGGGGGTGTTCTTGGCTATATATTGTGTTCAACTCAGGAAGCTGTTGTTATTCCTCCACATGTTGTCTTTTCAATAAGACCAAATCCAGGATATTGGGAATTTGTTAAGGTCAGCTCTGAGGATCTCTCAGTTGAGGCCATCACTGTCCGAGACTTCTTGAAATACAAAGAAGCTTTATATGATGAGAAATGGTCGAATGATGAACATGTATTGGAGGTGGATTTTAGAGCAATTGATTTCTCTACTCCTCACTTGACTCTATCTTCCTCGCAACTTATTCTAAATGAGACCCTCAACACGGCTTCGAAGCTTCAGGCGGCACTGATTGTAACGGAAGTGTACCTCTCAGATCTCCCTAAGGACACACCGTTCCAAAATTTTGAACTAAGTTTTAAGGAGTGGGGCTTTGAGAAGGGGTGGGGAGACACTGCAGAAAGAACAAAGGAGACCATGAAAATACTTTTGGAAGTACTTCAAGCTCCAGACCCGTTGAACATGGATAGGTTTTTCAGTAGGCTTCCCACAATATTCAATGTCGTAATATTCTCTCCCCATGGCTACTTTGGCCAAGCAGATGTTCTTGGCTTACCAGACACAGGCGGGCAGGTGGTTTACATTCTGGATCAAGTGAAAGCTATGGAGGAAGAATTGACTCTGAGAATTAAGCAACAAGGACTTACTGTGAAGCCTCAAATTCTTGTGGTGACAAGACTCATACCCGAAGCAAGGGGAACTAAGTGCAACCAGGAGTTGGAACCAATCAATGGCACCAAGTACTCTAACATCCTTAGGGTGCCATTTAGGACAGAAAAGGGTATCCTGCGCCGTTGGGTTTCTCGTTTTGACATCTATCCCTATCTTGAGTTGTTTACACAGGCATGTTTTCCGTCGATATTTAAATTCATACTTTTAGAGTGCTCAATTATTGATTTTGATGCTACGGCCAAAATTCTCAACCTGATGGAAGGAAAGCCGGATCTTATTATTGGAAACTACACAGATGGCAATTTGGTGGCTTCTCTCATGGCTAATAAACTTGGGATAACTCAGGCAACTATTGCTCATGCTTTGGAGAAGACCAAGTACGAAGATTCAGACATCAACTGGAAGGAGTTAGATCCCAAGTATCACTTCTCATGCCAATTTCTTGCTGACACAATCTCAATGAATGCTACAGATTTTGTCATTGCAAGCACATACCAGGAAATTGCAGGAAGCAAGGACAGACCAGGACAATACGAAAGTCATACTGCATTTACACTCCCGGGGCTCTGTAGAGTTGTTTCTGGCATCAATGTATTTGACCCCAAATTCAACATTGCCGCTCCTGGGGCAGATCAGTCTGTATATTTCCCCTACTCAGACAAGCAGAAACGGCTCACTTCATTCTATCCTGCCATTGAAGAACTACTGTTTAGTAAAGAGGATAGCAGCCCGGCTGAAATCATCGTTGATGGGGTCTCTGGTTTCCATATTGATCCTAACAACGGTGATGAAGCAAGCAAAAAAATCGCTGATTTCTTTGAGAACTCCAAGACTGATGCAGCATACTGGGACAGATTTTCCAAAGCAGGCTTGCAGCGCATATACGAATGCTACACTTGGAAGATATATGCAAACAAGGTGTTGAACATGGGAAGCACTTATACTTTCTGGAGGCAGTTGAACAAAGAGCAGAAACAGGCAAAGCAAAGATACATCCAGATGTTCTTTAATCTCCAATATAGGAACCTGGTGAAGAATGTGCCTGTCCCACGTGATGAACCTGAACAACCACAAACCACATCAAGCACAAGGCGCTCACAGTCTCTATTGCAAAGGTTGTTTGGAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005623 GO:0008150 GO:0008194 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0030312 GO:0033036 GO:0033037 GO:0035251 GO:0044464 GO:0046527 GO:0051179 GO:0052545 GO:0071944 GO:0080165

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

678

Amino Acids

77.29

Weight (kDa)

5.82

Isoelectric Point (pI)

42.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_N	PF24861	9 - 123	8.2e-47	Sucrose synthase N-terminal
SUS_EPBD	PF24862	152 - 208	6.3e-16	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	231 - 538	1.2e-164	Sucrose synthase first GT-B domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004012)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37180 AT5G37180 AT5G37180
fragaria_vesca	FvH4_1g27400 FvH4_1g27400 FvH4_1g27400 FvH4_1g27400 FvH4_1g27400 FvH4_1g27400
malus_domestica	MD09G1280200.v1.1 MD17G1286900.v1.1 MD17G1287000.v1.1
prunus_persica	Prupe.3G014100_v2.0.a1 Prupe.3G014100_v2.0.a1 Prupe.3G014100_v2.0.a1
pyrus_communis	pycom09g18830 pycom17g27030 pycom17g27040
rosa_chinensis	RchiOBHm_Chr3g0488651
rosa_laevigata	RLG00000022933
rosa_multiflora	Rmu_sc0005363.1_g000051
rosa_roxburghii	Rroxscaffold_6G00393980
rosa_rugosa	Rorug03G0240100 Rorug03G0240100 Rorug03G0240200 Rorug03G0240200 Rorug03G0240200 Rorug03G0240200
rosa_samantha	Rh3AG288800 Rh3BG325400 Rh3CG323100 Rh3DG321800
rosa_wichuraiana	Rw3G025570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	793
Acc36I	ACCTGC	1 cut(s)	793
AccB1I	GGYRCC	2 cut(s)	965, 991
AccB7I	CCANNNNNTGG	3 cut(s)	196, 949, 962
AccBSI	CCGCTC	1 cut(s)	1523
AciI	CCGC	5 cut(s)	14, 88, 515, 798, 1521
AclWI	GGATC	5 cut(s)	327, 828, 1182, 1310, 1670
AcoI	YGGCCR	3 cut(s)	15, 763, 1140
AcsI	RAATTY	6 cut(s)	296, 574, 891, 1097, 1146, 1508
AcuI	CTGAAG	1 cut(s)	494
AcvI	CACGTG	1 cut(s)	1955
AcyI	GRCGYC	1 cut(s)	57
AfaI	GTAC	5 cut(s)	104, 539, 669, 974, 1283
AfiI	CCNNNNNNNGG	9 cut(s)	196, 284, 496, 794, 924, 949, 962, 1527, 1685
AflIII	ACRYGT	2 cut(s)	256, 403
AjnI	CCWGG	6 cut(s)	283, 942, 1398, 1426, 1525, 1929
AjuI	GAANNNNNNNTTGG	6 cut(s)	644, 676, 759, 791, 1313, 1345
AloI	GAACNNNNNNTCC	2 cut(s)	551, 583
AluBI	AGCT	8 cut(s)	41, 133, 239, 312, 371, 508, 677, 833
AluI	AGCT	8 cut(s)	41, 133, 239, 312, 371, 508, 677, 833
Alw21I	GWGCWC	1 cut(s)	1118
Alw26I	GTCTC	7 cut(s)	152, 342, 479, 615, 638, 1663, 2010
AlwI	GGATC	5 cut(s)	327, 828, 1182, 1310, 1670
Ama87I	CYCGRG	1 cut(s)	1462
AoxI	GGCC	4 cut(s)	15, 333, 763, 1140
ApeKI	GCWGC	4 cut(s)	11, 1629, 1742, 1777
ApoI	RAATTY	6 cut(s)	296, 574, 891, 1097, 1146, 1508
Asp700I	GAANNNNTTC	1 cut(s)	669
AspLEI	GCGC	3 cut(s)	1023, 1782, 1998
AsuC2I	CCSGG	3 cut(s)	1463, 1464, 1634
AsuHPI	GGTGA	4 cut(s)	128, 913, 1700, 1945
AsuII	TTCGAA	1 cut(s)	503
AvaI	CYCGRG	1 cut(s)	1462
AxyI	CCTNAGG	3 cut(s)	62, 555, 986
BalI	TGGCCA	1 cut(s)	765
BanI	GGYRCC	2 cut(s)	965, 991
BanII	GRGCYC	1 cut(s)	1470
BarI	GAAGNNNNNNTAC	4 cut(s)	1265, 1297, 1828, 1860
BbrPI	CACGTG	1 cut(s)	1955
BbsI	GAAGAC	1 cut(s)	1280
Bbv12I	GWGCWC	1 cut(s)	1118
BbvI	GCAGC	4 cut(s)	23, 1641, 1754, 1789
BccI	CCATC	4 cut(s)	344, 1153, 1193, 1646
BceAI	ACGGC	6 cut(s)	2, 57, 513, 1008, 1155, 1589
BcgI	CGANNNNNNTGC	2 cut(s)	1690, 1724
BciT130I	CCWGG	6 cut(s)	285, 944, 1400, 1428, 1527, 1931
BciVI	GTATCC	1 cut(s)	1025
BcnI	CCSGG	3 cut(s)	1463, 1464, 1634
BcoDI	GTCTC	7 cut(s)	152, 342, 479, 615, 638, 1663, 2010
BfmI	CTRYAG	4 cut(s)	9, 627, 1371, 1471
BfoI	RGCGCY	1 cut(s)	1999
BfuAI	ACCTGC	1 cut(s)	793
BfuI	GTATCC	1 cut(s)	1025
BglII	AGATCT	1 cut(s)	547
BisI	GCNGC	7 cut(s)	12, 15, 516, 1521, 1630, 1743, 1778
BlsI	GCNGC	7 cut(s)	13, 16, 517, 1522, 1631, 1744, 1779
BmcAI	AGTACT	2 cut(s)	669, 974
Bme1390I	CCNGG	9 cut(s)	285, 944, 1400, 1428, 1463, 1464, 1527, 1634, 1931
BmeT110I	CYCGRG	1 cut(s)	1462
BmiI	GGNNCC	4 cut(s)	954, 967, 993, 1928
BmrFI	CCNGG	9 cut(s)	285, 944, 1400, 1428, 1463, 1464, 1527, 1634, 1931
BmrI	ACTGGG	1 cut(s)	1759
BmsI	GCATC	3 cut(s)	1123, 1497, 1729
BmuI	ACTGGG	1 cut(s)	1759
BpiI	GAAGAC	1 cut(s)	1280
BplI	GAGNNNNNCTC	2 cut(s)	308, 340
BpmI	CTGGAG	2 cut(s)	663, 1873
Bpu14I	TTCGAA	1 cut(s)	503
BpuEI	CTTGAG	1 cut(s)	1079
BpuMI	CCSGG	3 cut(s)	1463, 1464, 1634
BsaAI	YACGTR	1 cut(s)	1955
BsaHI	GRCGYC	1 cut(s)	57
BsaI	GGTCTC	3 cut(s)	479, 638, 1663
BsaJI	CCNNGG	4 cut(s)	751, 1462, 1463, 1526
Bsc4I	CCNNNNNNNGG	9 cut(s)	196, 284, 496, 794, 924, 949, 962, 1527, 1685
Bse1I	ACTGG	2 cut(s)	1307, 1754
Bse21I	CCTNAGG	3 cut(s)	62, 555, 986
Bse3DI	GCAATG	2 cut(s)	1383, 1515
BseBI	CCWGG	6 cut(s)	285, 944, 1400, 1428, 1527, 1931
BseDI	CCNNGG	4 cut(s)	751, 1462, 1463, 1526
BseGI	GGATG	2 cut(s)	981, 1896
BseLI	CCNNNNNNNGG	9 cut(s)	196, 284, 496, 794, 924, 949, 962, 1527, 1685
BseMI	GCAATG	2 cut(s)	1383, 1515
BseMII	CTCAG	8 cut(s)	53, 245, 306, 339, 558, 845, 1259, 1572
BseNI	ACTGG	2 cut(s)	1307, 1754
BseRI	GAGGAG	1 cut(s)	435
BseX3I	CGGCCG	1 cut(s)	15
BseXI	GCAGC	4 cut(s)	23, 1641, 1754, 1789
Bsh1285I	CGRYCG	1 cut(s)	18
BshFI	GGCC	4 cut(s)	17, 335, 765, 1142
BshNI	GGYRCC	2 cut(s)	965, 991
BsiEI	CGRYCG	1 cut(s)	18
BsiHKAI	GWGCWC	1 cut(s)	1118
BsiHKCI	CYCGRG	1 cut(s)	1462
BsiSI	CCGG	4 cut(s)	53, 1172, 1463, 1634
BslFI	GGGAC	2 cut(s)	1766, 1934
BslI	CCNNNNNNNGG	9 cut(s)	196, 284, 496, 794, 924, 949, 962, 1527, 1685
BsmAI	GTCTC	7 cut(s)	152, 342, 479, 615, 638, 1663, 2010
BsmFI	GGGAC	2 cut(s)	1766, 1934
BsmI	GAATGC	2 cut(s)	1372, 1796
BsnI	GGCC	4 cut(s)	17, 335, 765, 1142
Bso31I	GGTCTC	3 cut(s)	479, 638, 1663
BsoBI	CYCGRG	1 cut(s)	1462
Bsp119I	TTCGAA	1 cut(s)	503
Bsp1286I	GDGCHC	2 cut(s)	1118, 1470
Bsp1407I	TGTACA	1 cut(s)	102
Bsp143I	GATC	7 cut(s)	319, 547, 820, 1174, 1315, 1534, 1675
Bsp19I	CCATGG	1 cut(s)	751
BspACI	CCGC	5 cut(s)	14, 88, 515, 798, 1521
BspANI	GGCC	4 cut(s)	17, 335, 765, 1142
BspCNI	CTCAG	8 cut(s)	54, 244, 307, 338, 557, 846, 1258, 1571
BspLI	GGNNCC	4 cut(s)	954, 967, 993, 1928
BspMAI	CTGCAG	2 cut(s)	13, 631
BspMI	ACCTGC	1 cut(s)	793
BspPI	GGATC	5 cut(s)	327, 828, 1182, 1310, 1670
BspT104I	TTCGAA	1 cut(s)	503
BspT107I	GGYRCC	2 cut(s)	965, 991
BspTNI	GGTCTC	3 cut(s)	479, 638, 1663
BsrBI	CCGCTC	1 cut(s)	1523
BsrDI	GCAATG	2 cut(s)	1383, 1515
BsrGI	TGTACA	1 cut(s)	102
BsrI	ACTGG	2 cut(s)	1307, 1754
BssECI	CCNNGG	4 cut(s)	751, 1462, 1463, 1526
BssMI	GATC	7 cut(s)	319, 547, 820, 1174, 1315, 1534, 1675
BssNI	GRCGYC	1 cut(s)	57
BssT1I	CCWWGG	1 cut(s)	751
Bst2UI	CCWGG	6 cut(s)	285, 944, 1400, 1428, 1527, 1931
Bst4CI	ACNGT	7 cut(s)	163, 343, 567, 880, 1611, 1688, 2004
BstACI	GRCGYC	1 cut(s)	57
BstAPI	GCANNNNNTGC	1 cut(s)	1256
BstAUI	TGTACA	1 cut(s)	102
BstBAI	YACGTR	1 cut(s)	1955
BstBI	TTCGAA	1 cut(s)	503
BstC8I	GCNNGC	5 cut(s)	800, 1390, 1699, 1771, 1775
BstDSI	CCRYGG	1 cut(s)	751
BstF5I	GGATG	2 cut(s)	981, 1896
BstH2I	RGCGCY	1 cut(s)	1999
BstHHI	GCGC	3 cut(s)	1023, 1782, 1998
BstKTI	GATC	7 cut(s)	322, 550, 823, 1177, 1318, 1537, 1678
BstMAI	GTCTC	7 cut(s)	152, 342, 479, 615, 638, 1663, 2010
BstMBI	GATC	7 cut(s)	319, 547, 820, 1174, 1315, 1534, 1675
BstMCI	CGRYCG	1 cut(s)	18
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 762, 1256, 1529, 1573, 1779, 1995
BstNI	CCWGG	6 cut(s)	285, 944, 1400, 1428, 1527, 1931
BstNSI	RCATGY	3 cut(s)	260, 407, 1080
BstSCI	CCNGG	9 cut(s)	283, 942, 1398, 1426, 1461, 1462, 1525, 1632, 1929
BstSFI	CTRYAG	4 cut(s)	9, 627, 1371, 1471
BstV1I	GCAGC	4 cut(s)	23, 1641, 1754, 1789
BstV2I	GAAGAC	1 cut(s)	1280
BstX2I	RGATCY	4 cut(s)	319, 547, 1174, 1315
BstXI	CCANNNNNNTGG	1 cut(s)	291
BstYI	RGATCY	4 cut(s)	319, 547, 1174, 1315
BstZI	CGGCCG	1 cut(s)	15
Bsu36I	CCTNAGG	3 cut(s)	62, 555, 986
BsuI	GTATCC	1 cut(s)	1025
BsuRI	GGCC	4 cut(s)	17, 335, 765, 1142
BtgI	CCRYGG	1 cut(s)	751
BtsCI	GGATG	2 cut(s)	981, 1896
BtsI	GCAGTG	1 cut(s)	624
BtsIMutI	CAGTG	3 cut(s)	339, 518, 624
BveI	ACCTGC	1 cut(s)	793
Cac8I	GCNNGC	5 cut(s)	800, 1390, 1699, 1771, 1775
CfoI	GCGC	3 cut(s)	1023, 1782, 1998
Cfr9I	CCCGGG	1 cut(s)	1462
CseI	GACGC	1 cut(s)	65
CsiI	ACCWGGT	1 cut(s)	1929
Csp6I	GTAC	5 cut(s)	103, 538, 668, 973, 1282
CviQI	GTAC	5 cut(s)	103, 538, 668, 973, 1282
DpnI	GATC	7 cut(s)	321, 549, 822, 1176, 1317, 1536, 1677
DpnII	GATC	7 cut(s)	319, 547, 820, 1174, 1315, 1534, 1675
DraI	TTTAAA	1 cut(s)	1096
EaeI	YGGCCR	3 cut(s)	15, 763, 1140
EagI	CGGCCG	1 cut(s)	15
EclXI	CGGCCG	1 cut(s)	15
Eco130I	CCWWGG	1 cut(s)	751
Eco24I	GRGCYC	1 cut(s)	1470
Eco31I	GGTCTC	3 cut(s)	479, 638, 1663
Eco52I	CGGCCG	1 cut(s)	15
Eco57I	CTGAAG	1 cut(s)	494
Eco72I	CACGTG	1 cut(s)	1955
Eco81I	CCTNAGG	3 cut(s)	62, 555, 986
Eco88I	CYCGRG	1 cut(s)	1462
EcoRII	CCWGG	6 cut(s)	283, 942, 1398, 1426, 1525, 1929
EcoT14I	CCWWGG	1 cut(s)	751
EcoT38I	GRGCYC	1 cut(s)	1470
ErhI	CCWWGG	1 cut(s)	751
FalI	AAGNNNNNCTT	4 cut(s)	77, 109, 1313, 1345
FaqI	GGGAC	2 cut(s)	1766, 1934
FauI	CCCGC	1 cut(s)	791
Fnu4HI	GCNGC	7 cut(s)	12, 15, 516, 1521, 1630, 1743, 1778
FokI	GGATG	2 cut(s)	968, 1883
FriOI	GRGCYC	1 cut(s)	1470
Fsp4HI	GCNGC	7 cut(s)	12, 15, 516, 1521, 1630, 1743, 1778
GlaI	GCGC	3 cut(s)	1022, 1781, 1997
GluI	GCNGC	7 cut(s)	12, 15, 516, 1521, 1630, 1743, 1778
GsuI	CTGGAG	2 cut(s)	663, 1873
HaeII	RGCGCY	1 cut(s)	1999
HaeIII	GGCC	4 cut(s)	17, 335, 765, 1142
HapII	CCGG	4 cut(s)	53, 1172, 1463, 1634
HgaI	GACGC	1 cut(s)	65
HhaI	GCGC	3 cut(s)	1023, 1782, 1998
Hin1I	GRCGYC	1 cut(s)	57
Hin6I	GCGC	3 cut(s)	1021, 1780, 1996
HinP1I	GCGC	3 cut(s)	1021, 1780, 1996
HindIII	AAGCTT	3 cut(s)	131, 369, 506
HinfI	GANTC	5 cut(s)	32, 454, 850, 909, 1289
HpaII	CCGG	4 cut(s)	53, 1172, 1463, 1634
HphI	GGTGA	4 cut(s)	128, 913, 1700, 1945
Hpy166II	GTNNAC	3 cut(s)	538, 811, 1068
Hpy188I	TCNGA	7 cut(s)	31, 316, 347, 547, 855, 1294, 1561
Hpy188III	TCNNGA	8 cut(s)	109, 233, 355, 680, 818, 1058, 1852, 1900
Hpy8I	GTNNAC	3 cut(s)	538, 811, 1068
Hpy99I	CGWCG	1 cut(s)	1090
HpyAV	CCTTC	4 cut(s)	114, 604, 1157, 1300
HpyCH4III	ACNGT	7 cut(s)	163, 343, 567, 880, 1611, 1688, 2004
HpyCH4IV	ACGT	1 cut(s)	1954
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 762, 1256, 1529, 1573, 1779, 1995
HpySE526I	ACGT	1 cut(s)	1954
Hsp92I	GRCGYC	1 cut(s)	57
HspAI	GCGC	3 cut(s)	1021, 1780, 1996
Kzo9I	GATC	7 cut(s)	319, 547, 820, 1174, 1315, 1534, 1675
LmnI	GCTCC	3 cut(s)	32, 682, 1528
Lsp1109I	GCAGC	4 cut(s)	23, 1641, 1754, 1789
LweI	GCATC	3 cut(s)	1123, 1497, 1729
MabI	ACCWGGT	1 cut(s)	1929
MaeII	ACGT	1 cut(s)	1954
MaeIII	GTNAC	3 cut(s)	74, 526, 901
MalI	GATC	7 cut(s)	321, 549, 822, 1176, 1317, 1536, 1677
MbiI	CCGCTC	1 cut(s)	1523
MboI	GATC	7 cut(s)	319, 547, 820, 1174, 1315, 1534, 1675
MboII	GAAGA	8 cut(s)	129, 453, 854, 1285, 1298, 1613, 1816, 1948
MfeI	CAATTG	1 cut(s)	429
MflI	RGATCY	4 cut(s)	319, 547, 1174, 1315
MhlI	GDGCHC	2 cut(s)	1118, 1470
MlsI	TGGCCA	1 cut(s)	765
MluNI	TGGCCA	1 cut(s)	765
MlyI	GAGTC	3 cut(s)	448, 844, 903
MmeI	TCCRAC	1 cut(s)	930
Mox20I	TGGCCA	1 cut(s)	765
MroXI	GAANNNNTTC	1 cut(s)	669
MscI	TGGCCA	1 cut(s)	765
MseI	TTAA	7 cut(s)	143, 303, 591, 861, 1095, 1911, 2032
MslI	CAYNNNNRTG	2 cut(s)	1075, 1901
Msp20I	TGGCCA	1 cut(s)	765
MspA1I	CMGCKG	1 cut(s)	14
MspI	CCGG	4 cut(s)	53, 1172, 1463, 1634
MspR9I	CCNGG	9 cut(s)	285, 944, 1400, 1428, 1463, 1464, 1527, 1634, 1931
MunI	CAATTG	1 cut(s)	429
Mva1269I	GAATGC	2 cut(s)	1372, 1796
MvaI	CCWGG	6 cut(s)	285, 944, 1400, 1428, 1527, 1931
MwoI	GCNNNNNNNGC	7 cut(s)	11, 762, 1256, 1529, 1573, 1779, 1995
NciI	CCSGG	3 cut(s)	1463, 1464, 1634
NcoI	CCATGG	1 cut(s)	751
NdeII	GATC	7 cut(s)	319, 547, 820, 1174, 1315, 1534, 1675
NlaIV	GGNNCC	4 cut(s)	954, 967, 993, 1928
NmuCI	GTSAC	1 cut(s)	901
NspI	RCATGY	3 cut(s)	260, 407, 1080
NspV	TTCGAA	1 cut(s)	503
PaqCI	CACCTGC	1 cut(s)	793
PciI	ACATGT	2 cut(s)	256, 403
PctI	GAATGC	2 cut(s)	1372, 1796
PdmI	GAANNNNTTC	1 cut(s)	669
PfeI	GAWTC	2 cut(s)	32, 1289
PflMI	CCANNNNNTGG	3 cut(s)	196, 949, 962
PfoI	TCCNGGA	1 cut(s)	283
PkrI	GCNGC	7 cut(s)	13, 16, 517, 1522, 1631, 1744, 1779
PleI	GAGTC	3 cut(s)	448, 844, 903
PmaCI	CACGTG	1 cut(s)	1955
PmlI	CACGTG	1 cut(s)	1955
PpsI	GAGTC	3 cut(s)	448, 844, 903
Ppu21I	YACGTR	1 cut(s)	1955
PscI	ACATGT	2 cut(s)	256, 403
Psp6I	CCWGG	6 cut(s)	283, 942, 1398, 1426, 1525, 1929
PspCI	CACGTG	1 cut(s)	1955
PspGI	CCWGG	6 cut(s)	283, 942, 1398, 1426, 1525, 1929
PspN4I	GGNNCC	4 cut(s)	954, 967, 993, 1928
PstI	CTGCAG	2 cut(s)	13, 631
PsuI	RGATCY	4 cut(s)	319, 547, 1174, 1315
RsaI	GTAC	5 cut(s)	104, 539, 669, 974, 1283
RsaNI	GTAC	5 cut(s)	103, 538, 668, 973, 1282
RseI	CAYNNNNRTG	2 cut(s)	1075, 1901
SaqAI	TTAA	7 cut(s)	143, 303, 591, 861, 1095, 1911, 2032
SatI	GCNGC	7 cut(s)	12, 15, 516, 1521, 1630, 1743, 1778
Sau3AI	GATC	7 cut(s)	319, 547, 820, 1174, 1315, 1534, 1675
ScaI	AGTACT	2 cut(s)	669, 974
SchI	GAGTC	3 cut(s)	448, 844, 903
ScrFI	CCNGG	9 cut(s)	285, 944, 1400, 1428, 1463, 1464, 1527, 1634, 1931
SduI	GDGCHC	2 cut(s)	1118, 1470
SexAI	ACCWGGT	1 cut(s)	1929
SfaNI	GCATC	3 cut(s)	1123, 1497, 1729
SfcI	CTRYAG	4 cut(s)	9, 627, 1371, 1471
SfuI	TTCGAA	1 cut(s)	503
SmaI	CCCGGG	1 cut(s)	1464
SmiI	ATTTAAAT	1 cut(s)	1096
SmiMI	CAYNNNNRTG	2 cut(s)	1075, 1901
SmlI	CTYRAG	1 cut(s)	1058
SmoI	CTYRAG	1 cut(s)	1058
SsiI	CCGC	5 cut(s)	14, 88, 515, 798, 1521
SspI	AATATT	2 cut(s)	726, 741
StyD4I	CCNGG	9 cut(s)	283, 942, 1398, 1426, 1461, 1462, 1525, 1632, 1929
StyI	CCWWGG	1 cut(s)	751
SwaI	ATTTAAAT	1 cut(s)	1096
TaaI	ACNGT	7 cut(s)	163, 343, 567, 880, 1611, 1688, 2004
TaiI	ACGT	1 cut(s)	1957
TaqI	TCGA	4 cut(s)	108, 392, 503, 1088
TatI	WGTACW	3 cut(s)	102, 667, 972
TauI	GCSGC	3 cut(s)	17, 518, 1523
TfiI	GAWTC	2 cut(s)	32, 1289
Tru1I	TTAA	7 cut(s)	143, 303, 591, 861, 1095, 1911, 2032
Tru9I	TTAA	7 cut(s)	143, 303, 591, 861, 1095, 1911, 2032
TscAI	CASTG	3 cut(s)	346, 525, 631
TseFI	GTSAC	1 cut(s)	901
TseI	GCWGC	4 cut(s)	11, 1629, 1742, 1777
Tsp45I	GTSAC	1 cut(s)	901
TspDTI	ATGAA	9 cut(s)	98, 143, 414, 665, 1090, 1379, 1572, 1707, 1974
TspGWI	ACGGA	2 cut(s)	545, 1074
TspMI	CCCGGG	1 cut(s)	1462
TspRI	CASTG	3 cut(s)	346, 525, 631
Van91I	CCANNNNNTGG	3 cut(s)	196, 949, 962
XapI	RAATTY	6 cut(s)	296, 574, 891, 1097, 1146, 1508
XceI	RCATGY	3 cut(s)	260, 407, 1080
XcmI	CCANNNNNNNNNTGG	1 cut(s)	758
XmaI	CCCGGG	1 cut(s)	1462
XmnI	GAANNNNTTC	1 cut(s)	669
ZrmI	AGTACT	2 cut(s)	669, 974

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.