Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0004012

Physical Location & Seq

Genomic Coordinates Reverse (-)

34320368 .. 34325758

Gene Structure 5391 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G025570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2652 bp

ATGGGTTCTGCAACTGCAGCCATGAAGCGATCTGACACCATAACCGAAAGCTTGCCCGACGCTCTGAGGCAGAGCCGGTACCATATGAAGAGGTGTTTTGCGAAGTTCATTGAAAAGGGAAAAAGGATAATGAAACTTCAGCATTTGATGAATGAAGTGGAGTTAGTCATAGATGACAAGGTTGAAATGAACCAAGTCTTGGCAGGAGTACTTGGCTATATACTGTGTTCAACTCAGGAAGCTGTTGTTAGTCCTCCACATGTTCTCTTTTCAATAAGACCAAACCCTGGATATTGGGAATTTGTTAAAGTCAGCTCTGAGGATCTCTCAGTTGAGGGCATCACTGTGAGGGACTACTTGAAATGCAAAGAAACACTTTATGATGAGAAATGGGCCAATGATGAAAATGCATTGGAGGTGGATTTTAGAGCAATTGACTTCTCTACTCCTCACTTAACTCTATCGTCCTCGATTGGAAATGGACTCGACTATGTTTCCAAGTTCATGACTTCGATGCTATCTGGGAGATTGGAGAATTCACAGCCCCTTGTGGACTACTTACTTTCACTGAATTATCAAGGAGAAAAACTTATGATCAATGACACCCTCAACACTGCTGCAAAGCTTCAGATGGCCATGATTGTGACAGAAGTTTACCTCTCGGGGCTTCCGAAGGACACTCCATTTCAAAATTTTGAGCTTAGGTTTAAGGAGTGGGGCTTTGAGAAGGGTTGGGGAGACACAGCAGAGAGATCCAAGGAAACAATGAGAATACTATCAGAAGTACTACAAGCTCCAGATCCACTTAACATGGAGAGGTTTTTGAGCAGGCTTCCTACCATATTCAATGTTGTAATATTCTCTCCTCATGGCTATTTTGGCCAAGCTGATGTTCTTGGTTTGCCAGACACCGGTGGACAGGTTGTTTATATTCTGGATCAAGTTAAAGCTCTGGAAGAAGAATTGCTGCTTAGAATTAAGCAACAAGGGCTTGCTGTGAAGCCTCAGATTTTTGTGGTAACAAGACTCATTCCTGAAGCAAGGGGAACCACGTGCAATCAGGAGTTGGAACCTATCAATGGCACCAAGTACTCAAACATTCTTAGGGTTCCATTTAGGACAGAAAAGGGGGTCCTTCGTCGTTGGGTTTCTCGTTTTGATATCTACCCCTATCTTGAGCTGTTTGTGCAGGATGCTACAGCCAAAATCCTCGACCTCATGGACGGGAAGCCTGATCTTATTATTGGAAATTACACTGATGGGAATTTGGTGGCCTCTCTGATGGCTATCAAACTTGGAATAACTCAGGCAACAATTGCACATGCTTTGGAGAAGACAAAGTATGAAGACTCAGACATCAAGTGGAAGGAATTAGACCCTAAGTATCACTTCTCATGCCAATTTCTGGCAGACACAATTTCGATGAATGCAACAGATTTTGTTATTGCAAGCACATTCCAGGAAATTGCAGGAAGGTTAGCAACATTAACTAATATATCATCATATAAGCAAGACAGACCAGGACAATATGAAAGCCATACCGCATTTACTCTCCCAGGGCTTTGTAGAGTTGTTTCAGGCATCAATGTATTCGATCCCAAATTTAACATTGCTGCACCTGGGGCTGACCAGTCTGTCTATTTTCCTTACACTGAGAAACAAAAAAGAGTCTCATCATTTCATCCTGCCATTGAAGAACTACTGTATAGCAAGGAGGATAACAATGAACATATGGGGTTTCTAGTGGATAGGAAGAAACCTATCATCTTCTCAATGGCAAGGCTTGACACTGTTAAGAACATTACTGGTTTAGTTGAATGGTATGGCAAAAACAAGAGGCTCAGAAATCTGGTTAACCTTGTAGTAGTTGGAGGCTTCTTTGACCCTTCAAAATCAAAAGATAGAGAGGAAATAGCAGAAATTAAAAAGATGCATTCACTGATAGAGAAGTACCAGCTCCAGGGTCAGATCAGATGGATAGCAGCACAGACTGATAGGAACCGCAATGGAGAACTCTACCGTTGTATTGCGGACACCAGGGGAGCTTTTGTCCAACCTGCTCTATATGAAGCTTTTGGTCTTACTGTCATTGAGGCAATGAACTGTGGTTTACCCACTTTTGCAACCAACCAAGGAGGGCCAGCTGAAATCATTGTTGATGGGATCTCTGGTTTCCATATTGATCCCAACAATGGTGATGATGCAAGCAGCAAAATTGCCGATTTCTTTGAGAAATGCAAAACTGAGGCTACATACTGGGACAATTATTCAAAAGCGGGTTTGCAACGCATTTATGAATGCTACACTTGGAAGATATATGCAAACAAGGTGTTGAACATGGGCTGCACTTATACATTCTGGAGGCAGTTGAACAAAGAGCAGAAACAGGCAAAGCAAAGATACATCCAGATGTTCTTTAATCTCCAATATAGAAATTTGGTGAAGAGTGTGCCTATCCCAAGTGACGAGCCTGAAAAACCAGCACCAAAGCCAGTCACCAAATCACAGACGACTCTAAGCACTAGACGCTCACAGTCCCGATTGCAAAGCAAAGGTGTTACTCATCTAGGATGTATGGTATCGGTAATTGCTGGAATGAACTGCTCCTGTTTTTTTAAGCTCGTCCTTATAAGGTTATTAGGACAACCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005623 GO:0008150 GO:0008194 GO:0016157 GO:0016740 GO:0016757 GO:0016758 GO:0030312 GO:0033036 GO:0033037 GO:0035251 GO:0044464 GO:0046527 GO:0051179 GO:0052545 GO:0071944 GO:0080165

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 Sucrose_synth

Protein Analysis

883

Amino Acids

100.15

Weight (kDa)

7.78

Isoelectric Point (pI)

38.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SUS_N	PF24861	10 - 124	5.4e-45	Sucrose synthase N-terminal
SUS_EPBD	PF24862	160 - 247	2.1e-27	Sucrose synthase EPBD domain
GT-B_Sucrose_synth	PF00862	270 - 569	2.8e-164	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	582 - 752	2.3e-30	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	587 - 742	6.2e-13	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004012)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37180 AT5G37180 AT5G37180
fragaria_vesca	FvH4_1g27400 FvH4_1g27400 FvH4_1g27400 FvH4_1g27400 FvH4_1g27400 FvH4_1g27400
malus_domestica	MD09G1280200.v1.1 MD17G1286900.v1.1 MD17G1287000.v1.1
prunus_persica	Prupe.3G014100_v2.0.a1 Prupe.3G014100_v2.0.a1 Prupe.3G014100_v2.0.a1
pyrus_communis	pycom09g18830 pycom17g27030 pycom17g27040
rosa_chinensis	RchiOBHm_Chr3g0488651
rosa_laevigata	RLG00000022933
rosa_multiflora	Rmu_sc0005363.1_g000051
rosa_roxburghii	Rroxscaffold_6G00393980
rosa_rugosa	Rorug03G0240100 Rorug03G0240100 Rorug03G0240200 Rorug03G0240200 Rorug03G0240200 Rorug03G0240200
rosa_samantha	Rh3AG288800 Rh3BG325400 Rh3CG323100 Rh3DG321800
rosa_wichuraiana	Rw3G025570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2630
AasI	GACNNNNNNGTC	1 cut(s)	1634
Acc36I	ACCTGC	1 cut(s)	2065
Acc65I	GGTACC	1 cut(s)	78
AccB1I	GGYRCC	2 cut(s)	78, 1082
AccB7I	CCANNNNNTGG	3 cut(s)	199, 287, 1405
AciI	CCGC	4 cut(s)	1542, 2002, 2030, 2276
AclWI	GGATC	7 cut(s)	330, 747, 794, 945, 1589, 2171, 2177
AcoI	YGGCCR	2 cut(s)	633, 880
AcsI	RAATTY	6 cut(s)	299, 535, 691, 1264, 1601, 2434
AcuI	CTGAAG	3 cut(s)	122, 611, 1056
AcvI	CACGTG	1 cut(s)	1053
AfaI	GTAC	5 cut(s)	80, 210, 786, 1091, 1952
AfiI	CCNNNNNNNGG	7 cut(s)	199, 287, 911, 1079, 1405, 1960, 2192
AflIII	ACRYGT	1 cut(s)	259
AgeI	ACCGGT	1 cut(s)	911
AjnI	CCWGG	7 cut(s)	286, 1458, 1519, 1555, 1618, 1959, 2036
AjuI	GAANNNNNNNTTGG	4 cut(s)	182, 214, 876, 908
Alw26I	GTCTC	2 cut(s)	732, 1675
AlwI	GGATC	7 cut(s)	330, 747, 794, 945, 1589, 2171, 2177
Ama87I	CYCGRG	1 cut(s)	661
AoxI	GGCC	5 cut(s)	393, 633, 880, 1272, 2138
ApeKI	GCWGC	7 cut(s)	17, 617, 967, 1613, 1982, 2208, 2343
ApoI	RAATTY	6 cut(s)	299, 535, 691, 1264, 1601, 2434
AsiGI	ACCGGT	1 cut(s)	911
Asp718I	GGTACC	1 cut(s)	78
AspS9I	GGNCC	3 cut(s)	393, 1132, 2138
AsuHPI	GGTGA	3 cut(s)	2207, 2452, 2488
AvaI	CYCGRG	1 cut(s)	661
AvaII	GGWCC	1 cut(s)	1132
BalI	TGGCCA	2 cut(s)	635, 882
BanI	GGYRCC	2 cut(s)	78, 1082
BbrPI	CACGTG	1 cut(s)	1053
BbsI	GAAGAC	2 cut(s)	1340, 1353
BbvI	GCAGC	7 cut(s)	29, 604, 954, 1600, 1994, 2220, 2330
BccI	CCATC	5 cut(s)	625, 1253, 1276, 1968, 2153
BciT130I	CCWGG	7 cut(s)	288, 1460, 1521, 1557, 1620, 1961, 2038
BclI	TGATCA	1 cut(s)	594
BcoDI	GTCTC	2 cut(s)	732, 1675
BfaI	CTAG	3 cut(s)	1742, 2523, 2567
BfmI	CTRYAG	2 cut(s)	15, 1197
BfuAI	ACCTGC	1 cut(s)	2065
BisI	GCNGC	7 cut(s)	18, 618, 968, 1614, 1983, 2209, 2344
BlsI	GCNGC	7 cut(s)	19, 619, 969, 1615, 1984, 2210, 2345
BmcAI	AGTACT	3 cut(s)	210, 786, 1091
Bme1390I	CCNGG	7 cut(s)	288, 1460, 1521, 1557, 1620, 1961, 2038
Bme18I	GGWCC	1 cut(s)	1132
BmeT110I	CYCGRG	1 cut(s)	661
BmgT120I	GGNCC	3 cut(s)	393, 1132, 2138
BmiI	GGNNCC	7 cut(s)	80, 1048, 1071, 1084, 1110, 1133, 2000
BmrFI	CCNGG	7 cut(s)	288, 1460, 1521, 1557, 1620, 1961, 2038
BmrI	ACTGGG	1 cut(s)	2266
BmsI	GCATC	6 cut(s)	348, 504, 1183, 1590, 1920, 2191
BmuI	ACTGGG	1 cut(s)	2266
BpiI	GAAGAC	2 cut(s)	1340, 1353
BplI	GAGNNNNNCTC	2 cut(s)	311, 343
BpmI	CTGGAG	3 cut(s)	780, 1943, 2380
Bpu10I	CCTNAGC	1 cut(s)	701
BpuEI	CTTGAG	1 cut(s)	1196
BsaAI	YACGTR	1 cut(s)	1053
BsaBI	GATNNNNATC	1 cut(s)	1286
BsaJI	CCNNGG	8 cut(s)	286, 756, 1555, 1556, 1619, 1960, 2037, 2131
BsaWI	WCCGGW	1 cut(s)	911
BsaXI	ACNNNNNCTCC	2 cut(s)	2034, 2064
Bsc4I	CCNNNNNNNGG	7 cut(s)	199, 287, 911, 1079, 1405, 1960, 2192
Bse118I	RCCGGY	2 cut(s)	75, 911
Bse1I	ACTGG	4 cut(s)	1630, 1810, 2261, 2492
Bse3DI	GCAATG	3 cut(s)	1608, 2011, 2103
Bse8I	GATNNNNATC	1 cut(s)	1286
BseBI	CCWGG	7 cut(s)	288, 1460, 1521, 1557, 1620, 1961, 2038
BseDI	CCNNGG	8 cut(s)	286, 756, 1555, 1556, 1619, 1960, 2037, 2131
BseGI	GGATG	4 cut(s)	1198, 1681, 2403, 2576
BseJI	GATNNNNATC	1 cut(s)	1286
BseLI	CCNNNNNNNGG	7 cut(s)	199, 287, 911, 1079, 1405, 1960, 2192
BseMI	GCAATG	3 cut(s)	1608, 2011, 2103
BseNI	ACTGG	4 cut(s)	1630, 1810, 2261, 2492
BseRI	GAGGAG	2 cut(s)	438, 855
BseXI	GCAGC	7 cut(s)	29, 604, 954, 1600, 1994, 2220, 2330
BsgI	GTGCAG	3 cut(s)	1208, 1599, 2329
BshFI	GGCC	5 cut(s)	395, 635, 882, 1274, 2140
BshNI	GGYRCC	2 cut(s)	78, 1082
BshTI	ACCGGT	1 cut(s)	911
BsiHKCI	CYCGRG	1 cut(s)	661
BsiSI	CCGG	2 cut(s)	76, 912
BslFI	GGGAC	3 cut(s)	365, 2273, 2521
BslI	CCNNNNNNNGG	7 cut(s)	199, 287, 911, 1079, 1405, 1960, 2192
BsmAI	GTCTC	2 cut(s)	732, 1675
BsmFI	GGGAC	3 cut(s)	365, 2273, 2521
BsmI	GAATGC	3 cut(s)	1432, 1933, 2303
BsnI	GGCC	5 cut(s)	395, 635, 882, 1274, 2140
BsoBI	CYCGRG	1 cut(s)	661
BspACI	CCGC	4 cut(s)	1542, 2002, 2030, 2276
BspANI	GGCC	5 cut(s)	395, 635, 882, 1274, 2140
BspHI	TCATGA	1 cut(s)	504
BspLI	GGNNCC	7 cut(s)	80, 1048, 1071, 1084, 1110, 1133, 2000
BspMAI	CTGCAG	1 cut(s)	19
BspMI	ACCTGC	1 cut(s)	2065
BspPI	GGATC	7 cut(s)	330, 747, 794, 945, 1589, 2171, 2177
BspT107I	GGYRCC	2 cut(s)	78, 1082
BsrDI	GCAATG	3 cut(s)	1608, 2011, 2103
BsrFI	RCCGGY	2 cut(s)	75, 911
BsrI	ACTGG	4 cut(s)	1630, 1810, 2261, 2492
BssAI	RCCGGY	2 cut(s)	75, 911
BssECI	CCNNGG	8 cut(s)	286, 756, 1555, 1556, 1619, 1960, 2037, 2131
BssT1I	CCWWGG	2 cut(s)	756, 2131
Bst2UI	CCWGG	7 cut(s)	288, 1460, 1521, 1557, 1620, 1961, 2038
Bst4CI	ACNGT	8 cut(s)	225, 346, 1704, 1792, 2021, 2086, 2105, 2535
Bst6I	CTCTTC	2 cut(s)	83, 2438
BstAPI	GCANNNNNTGC	1 cut(s)	1316
BstBAI	YACGTR	1 cut(s)	1053
BstC8I	GCNNGC	6 cut(s)	53, 830, 993, 1450, 2142, 2206
BstF5I	GGATG	4 cut(s)	1198, 1681, 2403, 2576
BstMAI	GTCTC	2 cut(s)	732, 1675
BstMWI	GCNNNNNNNGC	7 cut(s)	17, 879, 988, 1186, 1316, 1622, 2526
BstNI	CCWGG	7 cut(s)	288, 1460, 1521, 1557, 1620, 1961, 2038
BstNSI	RCATGY	2 cut(s)	263, 1325
BstSCI	CCNGG	7 cut(s)	286, 1458, 1519, 1555, 1618, 1959, 2036
BstSFI	CTRYAG	2 cut(s)	15, 1197
BstV1I	GCAGC	7 cut(s)	29, 604, 954, 1600, 1994, 2220, 2330
BstV2I	GAAGAC	2 cut(s)	1340, 1353
BstX2I	RGATCY	4 cut(s)	322, 752, 799, 2163
BstYI	RGATCY	4 cut(s)	322, 752, 799, 2163
BsuRI	GGCC	5 cut(s)	395, 635, 882, 1274, 2140
BtsCI	GGATG	4 cut(s)	1198, 1681, 2403, 2576
BtsI	GCAGTG	1 cut(s)	612
BtsIMutI	CAGTG	7 cut(s)	342, 566, 612, 1254, 1650, 1788, 1937
BveI	ACCTGC	1 cut(s)	2065
Cac8I	GCNNGC	6 cut(s)	53, 830, 993, 1450, 2142, 2206
CciI	TCATGA	1 cut(s)	504
Cfr10I	RCCGGY	2 cut(s)	75, 911
Cfr13I	GGNCC	3 cut(s)	393, 1132, 2138
CseI	GACGC	2 cut(s)	68, 2535
Csp6I	GTAC	5 cut(s)	79, 209, 785, 1090, 1951
CspAI	ACCGGT	1 cut(s)	911
CviQI	GTAC	5 cut(s)	79, 209, 785, 1090, 1951
DrdI	GACNNNNNNGTC	1 cut(s)	1634
DseDI	GACNNNNNNGTC	1 cut(s)	1634
EaeI	YGGCCR	2 cut(s)	633, 880
Eam1104I	CTCTTC	2 cut(s)	83, 2438
EarI	CTCTTC	2 cut(s)	83, 2438
Eco130I	CCWWGG	2 cut(s)	756, 2131
Eco32I	GATATC	1 cut(s)	1162
Eco47I	GGWCC	1 cut(s)	1132
Eco57I	CTGAAG	3 cut(s)	122, 611, 1056
Eco72I	CACGTG	1 cut(s)	1053
Eco88I	CYCGRG	1 cut(s)	661
EcoO109I	RGGNCCY	1 cut(s)	1132
EcoRI	GAATTC	1 cut(s)	535
EcoRII	CCWGG	7 cut(s)	286, 1458, 1519, 1555, 1618, 1959, 2036
EcoRV	GATATC	1 cut(s)	1162
EcoT14I	CCWWGG	2 cut(s)	756, 2131
EcoT22I	ATGCAT	2 cut(s)	412, 1935
ErhI	CCWWGG	2 cut(s)	756, 2131
FalI	AAGNNNNNCTT	4 cut(s)	360, 392, 1373, 1405
FaqI	GGGAC	3 cut(s)	365, 2273, 2521
FauI	CCCGC	1 cut(s)	2269
FauNDI	CATATG	2 cut(s)	84, 1731
FbaI	TGATCA	1 cut(s)	594
Fnu4HI	GCNGC	7 cut(s)	18, 618, 968, 1614, 1983, 2209, 2344
FokI	GGATG	4 cut(s)	1205, 1668, 2390, 2583
Fsp4HI	GCNGC	7 cut(s)	18, 618, 968, 1614, 1983, 2209, 2344
FspBI	CTAG	3 cut(s)	1742, 2523, 2567
GluI	GCNGC	7 cut(s)	18, 618, 968, 1614, 1983, 2209, 2344
GsuI	CTGGAG	3 cut(s)	780, 1943, 2380
HaeIII	GGCC	5 cut(s)	395, 635, 882, 1274, 2140
HapII	CCGG	2 cut(s)	76, 912
HgaI	GACGC	2 cut(s)	68, 2535
HincII	GTYRAC	1 cut(s)	1855
HindII	GTYRAC	1 cut(s)	1855
HindIII	AAGCTT	3 cut(s)	49, 623, 2070
HinfI	GANTC	5 cut(s)	483, 1026, 1349, 1668, 2512
HpaI	GTTAAC	1 cut(s)	1855
HpaII	CCGG	2 cut(s)	76, 912
HphI	GGTGA	3 cut(s)	2207, 2452, 2488
Hpy166II	GTNNAC	5 cut(s)	553, 655, 917, 1855, 2111
Hpy8I	GTNNAC	5 cut(s)	553, 655, 917, 1855, 2111
Hpy99I	CGWCG	2 cut(s)	62, 1143
HpyAV	CCTTC	6 cut(s)	667, 721, 1145, 1360, 1467, 1896
HpyCH4III	ACNGT	8 cut(s)	225, 346, 1704, 1792, 2021, 2086, 2105, 2535
HpyCH4IV	ACGT	1 cut(s)	1052
HpyF10VI	GCNNNNNNNGC	7 cut(s)	17, 879, 988, 1186, 1316, 1622, 2526
HpySE526I	ACGT	1 cut(s)	1052
KpnI	GGTACC	1 cut(s)	82
Ksp22I	TGATCA	1 cut(s)	594
KspAI	GTTAAC	1 cut(s)	1855
LmnI	GCTCC	4 cut(s)	799, 1962, 2042, 2609
Lsp1109I	GCAGC	7 cut(s)	29, 604, 954, 1600, 1994, 2220, 2330
LweI	GCATC	6 cut(s)	348, 504, 1183, 1590, 1920, 2191
MaeI	CTAG	3 cut(s)	1742, 2523, 2567
MaeII	ACGT	1 cut(s)	1052
MaeIII	GTNAC	5 cut(s)	643, 1018, 2462, 2494, 2557
MfeI	CAATTG	2 cut(s)	432, 1314
MflI	RGATCY	4 cut(s)	322, 752, 799, 2163
MlsI	TGGCCA	2 cut(s)	635, 882
MluNI	TGGCCA	2 cut(s)	635, 882
MlyI	GAGTC	5 cut(s)	477, 1020, 1343, 1677, 2506
MmeI	TCCRAC	3 cut(s)	1047, 1849, 2077
Mox20I	TGGCCA	2 cut(s)	635, 882
Mph1103I	ATGCAT	2 cut(s)	412, 1935
MscI	TGGCCA	2 cut(s)	635, 882
MslI	CAYNNNNRTG	3 cut(s)	344, 641, 2408
Msp20I	TGGCCA	2 cut(s)	635, 882
MspA1I	CMGCKG	1 cut(s)	2144
MspI	CCGG	2 cut(s)	76, 912
MspR9I	CCNGG	7 cut(s)	288, 1460, 1521, 1557, 1620, 1961, 2038
MunI	CAATTG	2 cut(s)	432, 1314
Mva1269I	GAATGC	3 cut(s)	1432, 1933, 2303
MvaI	CCWGG	7 cut(s)	288, 1460, 1521, 1557, 1620, 1961, 2038
MwoI	GCNNNNNNNGC	7 cut(s)	17, 879, 988, 1186, 1316, 1622, 2526
NdeI	CATATG	2 cut(s)	84, 1731
NlaIV	GGNNCC	7 cut(s)	80, 1048, 1071, 1084, 1110, 1133, 2000
NmuCI	GTSAC	3 cut(s)	643, 2462, 2494
NsiI	ATGCAT	2 cut(s)	412, 1935
NspI	RCATGY	2 cut(s)	263, 1325
PagI	TCATGA	1 cut(s)	504
PasI	CCCWGGG	1 cut(s)	1556
PciI	ACATGT	1 cut(s)	259
PcsI	WCGNNNNNNNCGW	1 cut(s)	668
PctI	GAATGC	3 cut(s)	1432, 1933, 2303
PflMI	CCANNNNNTGG	3 cut(s)	199, 287, 1405
PfoI	TCCNGGA	1 cut(s)	1458
PinAI	ACCGGT	1 cut(s)	911
PkrI	GCNGC	7 cut(s)	19, 619, 969, 1615, 1984, 2210, 2345
PleI	GAGTC	5 cut(s)	477, 1020, 1343, 1676, 2506
PmaCI	CACGTG	1 cut(s)	1053
PmlI	CACGTG	1 cut(s)	1053
PpsI	GAGTC	5 cut(s)	477, 1020, 1343, 1676, 2506
Ppu21I	YACGTR	1 cut(s)	1053
PpuMI	RGGWCCY	1 cut(s)	1132
PscI	ACATGT	1 cut(s)	259
PsiI	TTATAA	1 cut(s)	2630
Psp5II	RGGWCCY	1 cut(s)	1132
Psp6I	CCWGG	7 cut(s)	286, 1458, 1519, 1555, 1618, 1959, 2036
PspCI	CACGTG	1 cut(s)	1053
PspGI	CCWGG	7 cut(s)	286, 1458, 1519, 1555, 1618, 1959, 2036
PspN4I	GGNNCC	7 cut(s)	80, 1048, 1071, 1084, 1110, 1133, 2000
PspPI	GGNCC	3 cut(s)	393, 1132, 2138
PspPPI	RGGWCCY	1 cut(s)	1132
PstI	CTGCAG	1 cut(s)	19
PsuI	RGATCY	4 cut(s)	322, 752, 799, 2163
PvuII	CAGCTG	1 cut(s)	2144
RsaI	GTAC	5 cut(s)	80, 210, 786, 1091, 1952
RsaNI	GTAC	5 cut(s)	79, 209, 785, 1090, 1951
RseI	CAYNNNNRTG	3 cut(s)	344, 641, 2408
SatI	GCNGC	7 cut(s)	18, 618, 968, 1614, 1983, 2209, 2344
Sau96I	GGNCC	3 cut(s)	393, 1132, 2138
ScaI	AGTACT	3 cut(s)	210, 786, 1091
SchI	GAGTC	5 cut(s)	477, 1020, 1343, 1677, 2506
ScrFI	CCNGG	7 cut(s)	288, 1460, 1521, 1557, 1620, 1961, 2038
SfaNI	GCATC	6 cut(s)	348, 504, 1183, 1590, 1920, 2191
SfcI	CTRYAG	2 cut(s)	15, 1197
SgrAI	CRCCGGYG	1 cut(s)	911
SinI	GGWCC	1 cut(s)	1132
SmiMI	CAYNNNNRTG	3 cut(s)	344, 641, 2408
SmlI	CTYRAG	1 cut(s)	1175
SmoI	CTYRAG	1 cut(s)	1175
SsiI	CCGC	4 cut(s)	1542, 2002, 2030, 2276
SspI	AATATT	1 cut(s)	858
SspMI	CTAG	3 cut(s)	1742, 2523, 2567
StyD4I	CCNGG	7 cut(s)	286, 1458, 1519, 1555, 1618, 1959, 2036
StyI	CCWWGG	2 cut(s)	756, 2131
TaaI	ACNGT	8 cut(s)	225, 346, 1704, 1792, 2021, 2086, 2105, 2535
TaiI	ACGT	1 cut(s)	1055
TaqI	TCGA	6 cut(s)	470, 486, 512, 1212, 1421, 1593
TatI	WGTACW	3 cut(s)	208, 784, 1089
TscAI	CASTG	7 cut(s)	349, 573, 619, 1261, 1657, 1795, 1944
TseFI	GTSAC	3 cut(s)	643, 2462, 2494
TseI	GCWGC	7 cut(s)	17, 617, 967, 1613, 1982, 2208, 2343
Tsp45I	GTSAC	3 cut(s)	643, 2462, 2494
TspRI	CASTG	7 cut(s)	349, 573, 619, 1261, 1657, 1795, 1944
Van91I	CCANNNNNTGG	3 cut(s)	199, 287, 1405
VpaK11BI	GGWCC	1 cut(s)	1132
XapI	RAATTY	6 cut(s)	299, 535, 691, 1264, 1601, 2434
XceI	RCATGY	2 cut(s)	263, 1325
XspI	CTAG	3 cut(s)	1742, 2523, 2567
ZrmI	AGTACT	3 cut(s)	210, 786, 1091
Zsp2I	ATGCAT	2 cut(s)	412, 1935

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.