Rroxscaffold_6G00393980

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004012
Physical Location & Seq
Forward (+)
14317346 .. 14321012
3667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00393980.1

Sequence Viewer

Length: 2550 bp
ATGGATTCTGAAACTGCAGCCATGAAGCGATCTGACTCCATAGCCGAAAGCATGCCCGACGCTCTGAGGCAGAGCCGGTACCATATGAAGAGGTGTTTTGCTAAGTTCATTGAAAAGGGAAAAAGGATAATGAAACTTCAGCATTTGATGAATGAATTGGAGTTAGTCATAGATGACAAGGTTGAAATGAACCAAGTCTTGGCAGGAGTACTTGGCTATATATTGTGTTCAACTCAGGAAGCTGTTGTTAGTCCCCCACATGTTCTCTTTTCAATAAGACCAAACCCTGGATATTGGGAATTTGTTAAAGTCAGCGCTGAGGATCTCTCAGTTGAGGCCATCACCGTGAGGGACTACTTGAAATGCAAAGAAACGCTTTATGATGAGAAATGGGCCAATGATGAAAATGCTTTGGAGGTGGATTTTAGAGCAATTGACTTCTCTACTCCTCACTTAACTCTATCATCCTCGATTGGAAATGGACTCGACTATGTTTCCAAGTTCATGACTTCGATGCTAGCTGGGAGATTGGAGAATTCGCAGCCCCTTGTGGACTACTTACTTTCACTGAATTATCAAGGAGAACAACTTATGATCAATGACACCCTCAACACTGCTGCAAAGCTTCAGATGGCCATGATTGTGACAGAAGTTTACCTCTCGGCGCTTCCGAAGGACACTCCATTTCAAAATTTTGAAATTAGGTTTAAGGAGTGGGGCTTTGAGAAGGGTTGGGGAGACACAGCAGAGAGATCCAAGGAAACAATGAGAATACTATCAGAAGTACTACAAGCTCCAGATCCACTTAACATGGAGAGGTTTTTGAGCAGGCTTCCTACCATATTCAATGTTGTAATATTCTCTCCTCATGGCTATTTTGGCCAAGCTGATGTTCTTGGTTTGCCAGACACCGGTGGACAGGTTGTTTATATTCTGGATCAAGTTAAAGCTCTGGAAGAAGAATTGCTCCTTCGAATTAAGCAACAAGGGCTTGCTGTGAAGCCTCAGATTTTTGTGGTAACAAGACTCATTCCTGAAGCAAGGGGAACCACGTGCAATCAGGAGTTGGAACCGATCAATGGCACCAAGTACTCAAACATTCTTAGGGTTCCATTTAGGACAGAAAAGGGGGTCCTTCGTCGTTGGGTTTCTCGTTTTGATATCTACCCCTATCTTGAGCTGTTTGTGCAGGCATGGTTCCTCTTCAATATTTCAACTCATTCTTGTAGAGATGCTACAGCCAAAATCCTCGACCTCATGGACGGGAAGCCTGATCTTATTATTGGAAATTACTCTGATGGGAATTTGGTGGCCTCTCTGATGGCTAACAAACTTGGAATAACACAGGCAACAATTGCACATGCTTTGGAGAAGACCAAGTATGAAGACTCAGACGTCAAGTGGAAGGAATTAGACCCTAAGTATCACTTCTCATGCCAATTTCTGGCAGACACAATTTCGATGAATGCAACAGATTTTGTTATTGCAAGCACATTCCAGGAAATTGCAGGAAGCAAAGACAGACCGGGACAATATGAAAGCCATACCGCATTTACTCTCCCAGGGCTTTGTAGAGTTGTTTCAGGCATCAATGTATTCGATCCCAAATTTAACATTGCTGCACCTGGGGCTGACCAGTCTGTCTATTTTCCTTACACTGAGAAACAAAAAAGAATCTCATCATTTCATCCTGCCATTGAAGAACTACTGTATAGCAAGGAGGATAACAATGAACATATGGGGTTTCTAGTGGATAGGAAGAAACCTATCATCTTCTCAATGGCAAGGCTTGACACTGTTAAGAACATTACTGGTTTAGTTGAATGGTATGGCAAAAACAAGAGGCTCAGAAATCTGGTTAACCTTGTAGTAGTTGGAGGCTTCTTTGACCCTTCAAAATCAAAAGATAGAGAGGAAATAGCAGAAATTAAAAAGATGCATTCACTGATAGAGAAGTACCAGCTCCGGGGTCAGATCAGATGGATAGCAGCACAGACTGATAGGAACCGCAATGGAGAACTCTACCGTTGTATTGCGGACACCAGGGGAGCTTTTGTCCAACCTGCTCTATATGAAGCTTTTGGTCTTACTGTCATTGAGGCAATGAACTGTGGTTTACCCACTTTTGCAACCAACCAAGGAGGGCCAGCTGAAATCATTGTTGATGGGATCTCTGGTTTCCATATTGATCCCAACAATGGTGATGATGCAAGCAGCAAAATTGCCGATTTCTTTGAGAAATGCAAAACTGAGGCTACATACTGGGACAAGTATTCAAAAGCGGGTTTGCAACGCATTTATGAATGCTACACTTGGAAGATATATGCAAACAAGGTGTTGAACATGGGCTGCACTTATACATTCTGGAGGCAGTTGAACAAAGAGCAGAAACAGGCAAAGCAAAGATACATCCAGATGTTCTTTAATCTCCAATATAGAAATTTGGTGAAGAATGTGCCTATCCCAAGTGACGAGCCAGAAAAACCAGCACCAAAGCCAGTCACCAAATCACAGACGACTCTAAGGCATGTGATGCTAAAAGACATGTAG

Protein Analysis

849

Amino Acids

96.81

Weight (kDa)

6.45

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_N PF24861 10 - 124 4.7e-46 Sucrose synthase N-terminal
SUS_EPBD PF24862 160 - 247 1.2e-27 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 270 - 571 6.1e-166 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 584 - 758 2.3e-30 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 589 - 744 7.2e-13 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1394, 1640
AatII GACGTC 1 cut(s) 1398
Acc36I ACCTGC 1 cut(s) 2071
Acc65I GGTACC 1 cut(s) 78
AccB1I GGYRCC 2 cut(s) 78, 1082
AccB7I CCANNNNNTGG 3 cut(s) 199, 287, 1444
AciI CCGC 4 cut(s) 1548, 2008, 2036, 2282
AclWI GGATC 7 cut(s) 330, 747, 794, 945, 1595, 2177, 2183
AcoI YGGCCR 2 cut(s) 633, 880
AcsI RAATTY 6 cut(s) 299, 535, 691, 1303, 1607, 2440
AcuI CTGAAG 3 cut(s) 122, 611, 1056
AcvI CACGTG 1 cut(s) 1053
AcyI GRCGYC 1 cut(s) 1395
AfaI GTAC 5 cut(s) 80, 210, 786, 1091, 1958
AfeI AGCGCT 1 cut(s) 316
AfiI CCNNNNNNNGG 7 cut(s) 199, 287, 911, 1079, 1444, 1966, 2198
AflIII ACRYGT 2 cut(s) 259, 2544
AgeI ACCGGT 1 cut(s) 911
AjnI CCWGG 5 cut(s) 286, 1497, 1561, 1624, 2042
AjuI GAANNNNNNNTTGG 4 cut(s) 182, 214, 876, 908
Alw26I GTCTC 1 cut(s) 732
AlwI GGATC 7 cut(s) 330, 747, 794, 945, 1595, 2177, 2183
Aor51HI AGCGCT 1 cut(s) 316
AoxI GGCC 6 cut(s) 336, 393, 633, 880, 1311, 2144
ApeKI GCWGC 7 cut(s) 17, 541, 617, 1619, 1988, 2214, 2349
ApoI RAATTY 6 cut(s) 299, 535, 691, 1303, 1607, 2440
AsiGI ACCGGT 1 cut(s) 911
Asp718I GGTACC 1 cut(s) 78
AspLEI GCGC 2 cut(s) 317, 667
AspS9I GGNCC 3 cut(s) 393, 1132, 2144
AsuC2I CCSGG 2 cut(s) 1527, 1967
AsuHPI GGTGA 4 cut(s) 334, 2213, 2458, 2494
AsuII TTCGAA 1 cut(s) 973
AsuNHI GCTAGC 1 cut(s) 517
AvaII GGWCC 1 cut(s) 1132
BalI TGGCCA 2 cut(s) 635, 882
BanI GGYRCC 2 cut(s) 78, 1082
BbrPI CACGTG 1 cut(s) 1053
BbsI GAAGAC 2 cut(s) 1379, 1392
BbvCI CCTCAGC 1 cut(s) 318
BbvI GCAGC 7 cut(s) 29, 553, 604, 1606, 2000, 2226, 2336
BccI CCATC 6 cut(s) 347, 625, 1292, 1315, 1974, 2159
BciT130I CCWGG 5 cut(s) 288, 1499, 1563, 1626, 2044
BclI TGATCA 1 cut(s) 594
BcnI CCSGG 2 cut(s) 1527, 1967
BcoDI GTCTC 1 cut(s) 732
BfaI CTAG 2 cut(s) 518, 1748
BfmI CTRYAG 2 cut(s) 15, 1236
BfoI RGCGCY 2 cut(s) 318, 668
BfuAI ACCTGC 1 cut(s) 2071
BisI GCNGC 7 cut(s) 18, 542, 618, 1620, 1989, 2215, 2350
BlsI GCNGC 7 cut(s) 19, 543, 619, 1621, 1990, 2216, 2351
BmcAI AGTACT 3 cut(s) 210, 786, 1091
Bme1390I CCNGG 7 cut(s) 288, 1499, 1527, 1563, 1626, 1967, 2044
Bme18I GGWCC 1 cut(s) 1132
BmgT120I GGNCC 3 cut(s) 393, 1132, 2144
BmiI GGNNCC 8 cut(s) 80, 1048, 1071, 1084, 1110, 1133, 1199, 2006
BmrFI CCNGG 7 cut(s) 288, 1499, 1527, 1563, 1626, 1967, 2044
BmrI ACTGGG 1 cut(s) 2272
BmsI GCATC 6 cut(s) 504, 1222, 1596, 1926, 2197, 2523
BmtI GCTAGC 1 cut(s) 521
BmuI ACTGGG 1 cut(s) 2272
BpiI GAAGAC 2 cut(s) 1379, 1392
BplI GAGNNNNNCTC 2 cut(s) 311, 343
BpmI CTGGAG 2 cut(s) 780, 2386
Bpu10I CCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 973
BpuEI CTTGAG 1 cut(s) 1196
BpuMI CCSGG 2 cut(s) 1527, 1967
BsaAI YACGTR 1 cut(s) 1053
BsaHI GRCGYC 1 cut(s) 1395
BsaJI CCNNGG 8 cut(s) 286, 756, 1561, 1562, 1625, 1966, 2043, 2137
BsaWI WCCGGW 1 cut(s) 911
BsaXI ACNNNNNCTCC 2 cut(s) 2040, 2070
Bsc4I CCNNNNNNNGG 7 cut(s) 199, 287, 911, 1079, 1444, 1966, 2198
Bse118I RCCGGY 2 cut(s) 75, 911
Bse1I ACTGG 4 cut(s) 1636, 1816, 2267, 2498
Bse3DI GCAATG 3 cut(s) 1614, 2017, 2109
BseBI CCWGG 5 cut(s) 288, 1499, 1563, 1626, 2044
BseDI CCNNGG 8 cut(s) 286, 756, 1561, 1562, 1625, 1966, 2043, 2137
BseGI GGATG 3 cut(s) 464, 1687, 2409
BseLI CCNNNNNNNGG 7 cut(s) 199, 287, 911, 1079, 1444, 1966, 2198
BseMI GCAATG 3 cut(s) 1614, 2017, 2109
BseMII CTCAG 9 cut(s) 56, 248, 309, 342, 1019, 1404, 1650, 1861, 2241
BseNI ACTGG 4 cut(s) 1636, 1816, 2267, 2498
BseRI GAGGAG 2 cut(s) 438, 855
BseXI GCAGC 7 cut(s) 29, 553, 604, 1606, 2000, 2226, 2336
BseYI CCCAGC 1 cut(s) 521
BsgI GTGCAG 3 cut(s) 1208, 1605, 2335
BshFI GGCC 6 cut(s) 338, 395, 635, 882, 1313, 2146
BshNI GGYRCC 2 cut(s) 78, 1082
BshTI ACCGGT 1 cut(s) 911
BsiSI CCGG 4 cut(s) 76, 912, 1526, 1966
BslFI GGGAC 4 cut(s) 237, 365, 1542, 2279
BslI CCNNNNNNNGG 7 cut(s) 199, 287, 911, 1079, 1444, 1966, 2198
BsmAI GTCTC 1 cut(s) 732
BsmFI GGGAC 4 cut(s) 237, 365, 1542, 2279
BsmI GAATGC 3 cut(s) 1471, 1939, 2309
BsnI GGCC 6 cut(s) 338, 395, 635, 882, 1313, 2146
Bsp119I TTCGAA 1 cut(s) 973
BspACI CCGC 4 cut(s) 1548, 2008, 2036, 2282
BspANI GGCC 6 cut(s) 338, 395, 635, 882, 1313, 2146
BspCNI CTCAG 9 cut(s) 57, 247, 310, 341, 1018, 1403, 1651, 1860, 2242
BspHI TCATGA 1 cut(s) 504
BspLI GGNNCC 8 cut(s) 80, 1048, 1071, 1084, 1110, 1133, 1199, 2006
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 2071
BspOI GCTAGC 1 cut(s) 521
BspPI GGATC 7 cut(s) 330, 747, 794, 945, 1595, 2177, 2183
BspT104I TTCGAA 1 cut(s) 973
BspT107I GGYRCC 2 cut(s) 78, 1082
BsrDI GCAATG 3 cut(s) 1614, 2017, 2109
BsrFI RCCGGY 2 cut(s) 75, 911
BsrI ACTGG 4 cut(s) 1636, 1816, 2267, 2498
BssAI RCCGGY 2 cut(s) 75, 911
BssECI CCNNGG 8 cut(s) 286, 756, 1561, 1562, 1625, 1966, 2043, 2137
BssNI GRCGYC 1 cut(s) 1395
BssT1I CCWWGG 2 cut(s) 756, 2137
Bst2UI CCWGG 5 cut(s) 288, 1499, 1563, 1626, 2044
Bst4CI ACNGT 6 cut(s) 346, 1710, 1798, 2027, 2092, 2111
Bst6I CTCTTC 2 cut(s) 83, 1208
BstACI GRCGYC 1 cut(s) 1395
BstAPI GCANNNNNTGC 2 cut(s) 1355, 2533
BstBAI YACGTR 1 cut(s) 1053
BstBI TTCGAA 1 cut(s) 973
BstC8I GCNNGC 8 cut(s) 53, 519, 830, 993, 1191, 1489, 2148, 2212
BstF5I GGATG 3 cut(s) 464, 1687, 2409
BstH2I RGCGCY 2 cut(s) 318, 668
BstHHI GCGC 2 cut(s) 317, 667
BstMAI GTCTC 1 cut(s) 732
BstMWI GCNNNNNNNGC 6 cut(s) 879, 988, 1186, 1355, 1628, 2533
BstNI CCWGG 5 cut(s) 288, 1499, 1563, 1626, 2044
BstNSI RCATGY 5 cut(s) 55, 263, 1364, 2531, 2548
BstSCI CCNGG 7 cut(s) 286, 1497, 1525, 1561, 1624, 1965, 2042
BstSFI CTRYAG 2 cut(s) 15, 1236
BstV1I GCAGC 7 cut(s) 29, 553, 604, 1606, 2000, 2226, 2336
BstV2I GAAGAC 2 cut(s) 1379, 1392
BstX2I RGATCY 4 cut(s) 322, 752, 799, 2169
BstYI RGATCY 4 cut(s) 322, 752, 799, 2169
BsuRI GGCC 6 cut(s) 338, 395, 635, 882, 1313, 2146
BtsCI GGATG 3 cut(s) 464, 1687, 2409
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 5 cut(s) 566, 612, 1656, 1794, 1943
BveI ACCTGC 1 cut(s) 2071
Cac8I GCNNGC 8 cut(s) 53, 519, 830, 993, 1191, 1489, 2148, 2212
CciI TCATGA 1 cut(s) 504
CfoI GCGC 2 cut(s) 317, 667
Cfr10I RCCGGY 2 cut(s) 75, 911
Cfr13I GGNCC 3 cut(s) 393, 1132, 2144
CseI GACGC 1 cut(s) 68
Csp6I GTAC 5 cut(s) 79, 209, 785, 1090, 1957
CspAI ACCGGT 1 cut(s) 911
CviQI GTAC 5 cut(s) 79, 209, 785, 1090, 1957
DrdI GACNNNNNNGTC 2 cut(s) 1394, 1640
DseDI GACNNNNNNGTC 2 cut(s) 1394, 1640
EaeI YGGCCR 2 cut(s) 633, 880
Eam1104I CTCTTC 2 cut(s) 83, 1208
EarI CTCTTC 2 cut(s) 83, 1208
Eco130I CCWWGG 2 cut(s) 756, 2137
Eco32I GATATC 1 cut(s) 1162
Eco47I GGWCC 1 cut(s) 1132
Eco47III AGCGCT 1 cut(s) 316
Eco57I CTGAAG 3 cut(s) 122, 611, 1056
Eco72I CACGTG 1 cut(s) 1053
EcoO109I RGGNCCY 1 cut(s) 1132
EcoRI GAATTC 1 cut(s) 535
EcoRII CCWGG 5 cut(s) 286, 1497, 1561, 1624, 2042
EcoRV GATATC 1 cut(s) 1162
EcoT14I CCWWGG 2 cut(s) 756, 2137
EcoT22I ATGCAT 1 cut(s) 1941
ErhI CCWWGG 2 cut(s) 756, 2137
FalI AAGNNNNNCTT 4 cut(s) 360, 392, 1412, 1444
FaqI GGGAC 4 cut(s) 237, 365, 1542, 2279
FauI CCCGC 1 cut(s) 2275
FauNDI CATATG 2 cut(s) 84, 1737
FbaI TGATCA 1 cut(s) 594
Fnu4HI GCNGC 7 cut(s) 18, 542, 618, 1620, 1989, 2215, 2350
FokI GGATG 3 cut(s) 451, 1674, 2396
Fsp4HI GCNGC 7 cut(s) 18, 542, 618, 1620, 1989, 2215, 2350
FspBI CTAG 2 cut(s) 518, 1748
GlaI GCGC 2 cut(s) 316, 666
GluI GCNGC 7 cut(s) 18, 542, 618, 1620, 1989, 2215, 2350
GsaI CCCAGC 1 cut(s) 525
GsuI CTGGAG 2 cut(s) 780, 2386
HaeII RGCGCY 2 cut(s) 318, 668
HaeIII GGCC 6 cut(s) 338, 395, 635, 882, 1313, 2146
HapII CCGG 4 cut(s) 76, 912, 1526, 1966
HgaI GACGC 1 cut(s) 68
HhaI GCGC 2 cut(s) 317, 667
Hin1I GRCGYC 1 cut(s) 1395
Hin6I GCGC 2 cut(s) 315, 665
HinP1I GCGC 2 cut(s) 315, 665
HincII GTYRAC 1 cut(s) 1861
HindII GTYRAC 1 cut(s) 1861
HindIII AAGCTT 2 cut(s) 623, 2076
HinfI GANTC 7 cut(s) 5, 35, 483, 1026, 1388, 1674, 2518
HpaI GTTAAC 1 cut(s) 1861
HpaII CCGG 4 cut(s) 76, 912, 1526, 1966
HphI GGTGA 4 cut(s) 334, 2213, 2458, 2494
Hpy166II GTNNAC 5 cut(s) 553, 655, 917, 1861, 2117
Hpy8I GTNNAC 5 cut(s) 553, 655, 917, 1861, 2117
Hpy99I CGWCG 2 cut(s) 62, 1143
HpyAV CCTTC 6 cut(s) 667, 721, 980, 1145, 1399, 1902
HpyCH4III ACNGT 6 cut(s) 346, 1710, 1798, 2027, 2092, 2111
HpyCH4IV ACGT 2 cut(s) 1052, 1395
HpyF10VI GCNNNNNNNGC 6 cut(s) 879, 988, 1186, 1355, 1628, 2533
HpySE526I ACGT 2 cut(s) 1052, 1395
Hsp92I GRCGYC 1 cut(s) 1395
HspAI GCGC 2 cut(s) 315, 665
KpnI GGTACC 1 cut(s) 82
Ksp22I TGATCA 1 cut(s) 594
KspAI GTTAAC 1 cut(s) 1861
LmnI GCTCC 4 cut(s) 799, 972, 1968, 2048
Lsp1109I GCAGC 7 cut(s) 29, 553, 604, 1606, 2000, 2226, 2336
LweI GCATC 6 cut(s) 504, 1222, 1596, 1926, 2197, 2523
MaeI CTAG 2 cut(s) 518, 1748
MaeII ACGT 2 cut(s) 1052, 1395
MaeIII GTNAC 4 cut(s) 643, 1018, 2468, 2500
MfeI CAATTG 2 cut(s) 432, 1353
MflI RGATCY 4 cut(s) 322, 752, 799, 2169
MlsI TGGCCA 2 cut(s) 635, 882
MluNI TGGCCA 2 cut(s) 635, 882
MlyI GAGTC 5 cut(s) 29, 477, 1020, 1382, 2512
MmeI TCCRAC 3 cut(s) 1047, 1855, 2083
Mox20I TGGCCA 2 cut(s) 635, 882
Mph1103I ATGCAT 1 cut(s) 1941
MscI TGGCCA 2 cut(s) 635, 882
MslI CAYNNNNRTG 3 cut(s) 344, 641, 2414
Msp20I TGGCCA 2 cut(s) 635, 882
MspA1I CMGCKG 1 cut(s) 2150
MspI CCGG 4 cut(s) 76, 912, 1526, 1966
MspR9I CCNGG 7 cut(s) 288, 1499, 1527, 1563, 1626, 1967, 2044
MunI CAATTG 2 cut(s) 432, 1353
Mva1269I GAATGC 3 cut(s) 1471, 1939, 2309
MvaI CCWGG 5 cut(s) 288, 1499, 1563, 1626, 2044
MwoI GCNNNNNNNGC 6 cut(s) 879, 988, 1186, 1355, 1628, 2533
NciI CCSGG 2 cut(s) 1527, 1967
NdeI CATATG 2 cut(s) 84, 1737
NheI GCTAGC 1 cut(s) 517
NlaIV GGNNCC 8 cut(s) 80, 1048, 1071, 1084, 1110, 1133, 1199, 2006
NmeAIII GCCGAG 1 cut(s) 641
NmuCI GTSAC 3 cut(s) 643, 2468, 2500
NsiI ATGCAT 1 cut(s) 1941
NspI RCATGY 5 cut(s) 55, 263, 1364, 2531, 2548
NspV TTCGAA 1 cut(s) 973
PaeI GCATGC 1 cut(s) 55
PagI TCATGA 1 cut(s) 504
PasI CCCWGGG 1 cut(s) 1562
PciI ACATGT 2 cut(s) 259, 2544
PcsI WCGNNNNNNNCGW 1 cut(s) 668
PctI GAATGC 3 cut(s) 1471, 1939, 2309
PfeI GAWTC 2 cut(s) 5, 1674
PflMI CCANNNNNTGG 3 cut(s) 199, 287, 1444
PfoI TCCNGGA 1 cut(s) 1497
PinAI ACCGGT 1 cut(s) 911
PkrI GCNGC 7 cut(s) 19, 543, 619, 1621, 1990, 2216, 2351
PleI GAGTC 5 cut(s) 29, 477, 1020, 1382, 2512
PmaCI CACGTG 1 cut(s) 1053
PmlI CACGTG 1 cut(s) 1053
PpsI GAGTC 5 cut(s) 29, 477, 1020, 1382, 2512
Ppu21I YACGTR 1 cut(s) 1053
PpuMI RGGWCCY 1 cut(s) 1132
PscI ACATGT 2 cut(s) 259, 2544
Psp5II RGGWCCY 1 cut(s) 1132
Psp6I CCWGG 5 cut(s) 286, 1497, 1561, 1624, 2042
PspCI CACGTG 1 cut(s) 1053
PspFI CCCAGC 1 cut(s) 521
PspGI CCWGG 5 cut(s) 286, 1497, 1561, 1624, 2042
PspN4I GGNNCC 8 cut(s) 80, 1048, 1071, 1084, 1110, 1133, 1199, 2006
PspPI GGNCC 3 cut(s) 393, 1132, 2144
PspPPI RGGWCCY 1 cut(s) 1132
PstI CTGCAG 1 cut(s) 19
PsuI RGATCY 4 cut(s) 322, 752, 799, 2169
PvuII CAGCTG 1 cut(s) 2150
RsaI GTAC 5 cut(s) 80, 210, 786, 1091, 1958
RsaNI GTAC 5 cut(s) 79, 209, 785, 1090, 1957
RseI CAYNNNNRTG 3 cut(s) 344, 641, 2414
SatI GCNGC 7 cut(s) 18, 542, 618, 1620, 1989, 2215, 2350
Sau96I GGNCC 3 cut(s) 393, 1132, 2144
ScaI AGTACT 3 cut(s) 210, 786, 1091
SchI GAGTC 5 cut(s) 29, 477, 1020, 1382, 2512
ScrFI CCNGG 7 cut(s) 288, 1499, 1527, 1563, 1626, 1967, 2044
SfaNI GCATC 6 cut(s) 504, 1222, 1596, 1926, 2197, 2523
SfcI CTRYAG 2 cut(s) 15, 1236
SfuI TTCGAA 1 cut(s) 973
SgrAI CRCCGGYG 1 cut(s) 911
SinI GGWCC 1 cut(s) 1132
SmiMI CAYNNNNRTG 3 cut(s) 344, 641, 2414
SmlI CTYRAG 1 cut(s) 1175
SmoI CTYRAG 1 cut(s) 1175
SphI GCATGC 1 cut(s) 55
SsiI CCGC 4 cut(s) 1548, 2008, 2036, 2282
SspI AATATT 2 cut(s) 858, 1210
SspMI CTAG 2 cut(s) 518, 1748
StyD4I CCNGG 7 cut(s) 286, 1497, 1525, 1561, 1624, 1965, 2042
StyI CCWWGG 2 cut(s) 756, 2137
TaaI ACNGT 6 cut(s) 346, 1710, 1798, 2027, 2092, 2111
TaiI ACGT 2 cut(s) 1055, 1398
TaqI TCGA 7 cut(s) 470, 486, 512, 973, 1251, 1460, 1599
TatI WGTACW 3 cut(s) 208, 784, 1089
TfiI GAWTC 2 cut(s) 5, 1674
TscAI CASTG 5 cut(s) 573, 619, 1663, 1801, 1950
TseFI GTSAC 3 cut(s) 643, 2468, 2500
TseI GCWGC 7 cut(s) 17, 541, 617, 1619, 1988, 2214, 2349
Tsp45I GTSAC 3 cut(s) 643, 2468, 2500
TspRI CASTG 5 cut(s) 573, 619, 1663, 1801, 1950
Van91I CCANNNNNTGG 3 cut(s) 199, 287, 1444
VpaK11BI GGWCC 1 cut(s) 1132
XapI RAATTY 6 cut(s) 299, 535, 691, 1303, 1607, 2440
XceI RCATGY 5 cut(s) 55, 263, 1364, 2531, 2548
XspI CTAG 2 cut(s) 518, 1748
ZraI GACGTC 1 cut(s) 1396
ZrmI AGTACT 3 cut(s) 210, 786, 1091
Zsp2I ATGCAT 1 cut(s) 1941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.