RLG00000022933

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004012
Physical Location & Seq
Forward (+)
15393666 .. 15397903
4238 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022933

Sequence Viewer

Length: 2532 bp
ATGGATTCTGCAACTGCAGCCATGAAGCGATCTGACTCCATAGCCGAAAGCATGCCCGACGCTCTAAGGCAGAGCCGGTACCATATGAAGAGGTGTTTTGCTAAGTTCATTGAAAAGGGAAAAAGGATAATGAAACTTCAGCATTTGATGAATGAAGTGGAGTTAGTCATAGATGACAAGGTTGAAATGAACCAAGTCTTGGCAGGAGTACTTGGCTATATATTGTGTTCAACTCAGGAAGCTGTTGTTAGTCCCCCACATGTTCTCTTTTCAATAAGACCAAACCCTGGATATTGGGAATTTGTTAAAGTCAGCGCTGAGGATCTCTCAGTTGAGGCCATCACCGTGAGGGACTACTTGAAATGCAAAGAAACACTTTATGATGAGAAATGGGCCAATGATGAAAATGCTTTGGAGGTGGATTTTAGAGCAATTGACTTCTCTACTCCTCACTTAACTCTATCATCCTCGATTGGAAATGGACTTGACTATGTTTCAAAGTTCATGACTTCGATGCTAGCTGGGAGATTGGAGAATTCACAGCCCCTTGTGGACTACTTACTTTCACTGAATTATCAAGGAGAACAACTTATGATCAATGACACCCTCAACACTGCTGCAAAGCTTCAGATGGCCATGATTGTGACAGAAGTTTACCTCTCGGGGCTTCCGAAGGACACTCCATTTCAAAATTTTGAAATTAGGTTTAAGGAGTGGGGCTTTGAGAAGGGTTGGGGAGACACAGCAGAGAGATCCAAGGAAACAATGAGAATACTATCAGAAGTACTACAAGCTCCAGATCCACTTAACATGGAGAGGTTTTTGAGCAGGCTTCCTACCATATTCAATGTCGTAATATTCTCTCCTCATGGCTATTTTGGCCAAGCTGATGTTCTTGGTTTGCCAGACACCGGTGGACAGGTTGTTTATATTCTGGATCAAGTTAAAGCTCTGGAAGAAGAATTGCTCCTTAGAATTAAGCAACAAGGGCTTGCTGTGAAGCCTCAGATTTTTGTGGTAACAAGACTCATTCCTGAAGCAAGGGGAACCACGTGCAATCAGGAGTTAGAACCGATCAATGGCACCAAGTACTCAAACATTCTTAGGGTTCCATTTAGGACAGAAAAGGGGGTCCTTCGTCGTTGGGTTTCTCGTTTTGATATCTACCCCTATCTTGAGCTGTTTGTGCAGGATGCTACAGCCAAAATCCTCGACCTCATGGACGGGAAGCCTGATCTTATTATTGGAAATTACACTGATGGGAATTTGGTGGCCTCTCTGATGGCTAACAAACTTGGAATAACTCAGGCAACAATTGCACATGCTTTGGAGAAGACAAAGTATGAAGACTCAGACATCAAGTGGAAGGAATTAGACCCTAAGTATCACTTCTCATGCCAATTTCTGGCAGACACAATTTCGATGAATGCAACAGATTTTGTTATTGCAAGCACATTCCAGGAAATTGCAGGAAGCAAAGACAGACCAGGACAATATGAAAGCCATACCGCATTTACTCTCCCAGGGCTTTGTAGAGTTGTTTCAGGCATCAATGTATTCGATCCCAAATTTAACATTGCTGCACCTGGGGCTGACCAGTCTGTCTATTTTCCTTACACTGAGAAACAAAAAAGAGTCTCATCATTTCATCCTGCCATTGAAGAACTACTGTATAGCAAGGAGGATAACAATGAACATATGGGGTTTCTAATGGATAGGAAGAAACCTATCATCTTCTCAATGGCAAGGCTTGACACTGTTAAGAACATTACTGGTTTAGTTGAATGGTATGGCAAAAACAAGAGGCTCAGAAATCTGGTTAACCTTGTAGTAGTTGGAGGCTTCTTTGACCCTTCAAAATCAAAAGATAGAGAGGAAATAGCAGAAATTAAAAAGATGCATTCACTGATAGAGAAGTACCAGCTCCAGGGTCAGATCAGATGGATAGCAGCACAGACTGATAGGAACCGCAATGGAGAACTCTACCGTTGTATTGCGGACACCAGGGGAGCTTTTGTCCAACCTGCTCTATATGAAGCTTTTGGTCTAACGGTCATTGAGGCAATGAACTGTGGTTTACCCACTTTTGCAACCAACCAAGGAGGGCCAGCTGAAATCATTGTTGATGGGATCTCTGGTTTCCATATTGATCCGAACAATGGTGATGATGCAAGCAGCAAAATTGCCGATTTCTTTGAGAAATGCAAAACTGAGGCTACATACTGGGACAAGTATTCAAAAGCGGGTTTGCAACGCATTTATGAATGCTACACTTGGAAGATATATGCAAACAAGGTGTTGAACATGGGCTGCACTTATACATTCTGGAGGCAGTTGAACAAAGAGCAGAAACAGGCAAAGCAAAGATACATCCAGATGTTCTTTAATCTCCAATATAGAAATTTGGTGAAGAATGTGCCTATCCCAAGTGACGAGCCTGAAAAACCAGCACCAAAGCCAGTCACCAAATCACAGACTACTCTAAGCACTAGACGCTCACAGTCCCGATTGCAAAGGTTGTTTGGAGCCTGA

Protein Analysis

844

Amino Acids

95.92

Weight (kDa)

6.68

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_N PF24861 10 - 124 7.6e-46 Sucrose synthase N-terminal
SUS_EPBD PF24862 160 - 247 2.4e-27 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 270 - 558 1.8e-170 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 572 - 745 2.3e-30 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 576 - 731 5.7e-13 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1601
Acc36I ACCTGC 1 cut(s) 2032
Acc65I GGTACC 1 cut(s) 78
AccB1I GGYRCC 2 cut(s) 78, 1082
AccB7I CCANNNNNTGG 3 cut(s) 199, 287, 1405
AciI CCGC 4 cut(s) 1509, 1969, 1997, 2243
AclWI GGATC 7 cut(s) 330, 747, 794, 945, 1556, 2138, 2144
AcoI YGGCCR 2 cut(s) 633, 880
AcsI RAATTY 6 cut(s) 299, 535, 691, 1264, 1568, 2401
AcuI CTGAAG 3 cut(s) 122, 611, 1056
AcvI CACGTG 1 cut(s) 1053
AfaI GTAC 5 cut(s) 80, 210, 786, 1091, 1919
AfeI AGCGCT 1 cut(s) 316
AfiI CCNNNNNNNGG 7 cut(s) 199, 287, 911, 1079, 1405, 1927, 2159
AflIII ACRYGT 1 cut(s) 259
AgeI ACCGGT 1 cut(s) 911
AjnI CCWGG 7 cut(s) 286, 1458, 1486, 1522, 1585, 1926, 2003
AjuI GAANNNNNNNTTGG 4 cut(s) 182, 214, 876, 908
Alw26I GTCTC 2 cut(s) 732, 1642
AlwI GGATC 7 cut(s) 330, 747, 794, 945, 1556, 2138, 2144
Ama87I CYCGRG 1 cut(s) 661
Aor51HI AGCGCT 1 cut(s) 316
AoxI GGCC 6 cut(s) 336, 393, 633, 880, 1272, 2105
ApeKI GCWGC 6 cut(s) 17, 617, 1580, 1949, 2175, 2310
ApoI RAATTY 6 cut(s) 299, 535, 691, 1264, 1568, 2401
AsiGI ACCGGT 1 cut(s) 911
Asp718I GGTACC 1 cut(s) 78
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 3 cut(s) 393, 1132, 2105
AsuHPI GGTGA 4 cut(s) 334, 2174, 2419, 2455
AsuNHI GCTAGC 1 cut(s) 517
AvaI CYCGRG 1 cut(s) 661
AvaII GGWCC 1 cut(s) 1132
BalI TGGCCA 2 cut(s) 635, 882
BanI GGYRCC 2 cut(s) 78, 1082
BbrPI CACGTG 1 cut(s) 1053
BbsI GAAGAC 2 cut(s) 1340, 1353
BbvCI CCTCAGC 1 cut(s) 318
BbvI GCAGC 6 cut(s) 29, 604, 1567, 1961, 2187, 2297
BccI CCATC 6 cut(s) 347, 625, 1253, 1276, 1935, 2120
BciT130I CCWGG 7 cut(s) 288, 1460, 1488, 1524, 1587, 1928, 2005
BclI TGATCA 1 cut(s) 594
BcoDI GTCTC 2 cut(s) 732, 1642
BfaI CTAG 2 cut(s) 518, 2490
BfmI CTRYAG 2 cut(s) 15, 1197
BfoI RGCGCY 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 2032
BisI GCNGC 6 cut(s) 18, 618, 1581, 1950, 2176, 2311
BlsI GCNGC 6 cut(s) 19, 619, 1582, 1951, 2177, 2312
BmcAI AGTACT 3 cut(s) 210, 786, 1091
Bme1390I CCNGG 7 cut(s) 288, 1460, 1488, 1524, 1587, 1928, 2005
Bme18I GGWCC 1 cut(s) 1132
BmeT110I CYCGRG 1 cut(s) 661
BmgT120I GGNCC 3 cut(s) 393, 1132, 2105
BmiI GGNNCC 7 cut(s) 80, 1048, 1084, 1110, 1133, 1967, 2527
BmrFI CCNGG 7 cut(s) 288, 1460, 1488, 1524, 1587, 1928, 2005
BmrI ACTGGG 1 cut(s) 2233
BmsI GCATC 5 cut(s) 504, 1183, 1557, 1887, 2158
BmtI GCTAGC 1 cut(s) 521
BmuI ACTGGG 1 cut(s) 2233
BpiI GAAGAC 2 cut(s) 1340, 1353
BplI GAGNNNNNCTC 2 cut(s) 311, 343
BpmI CTGGAG 3 cut(s) 780, 1910, 2347
Bpu10I CCTNAGC 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 1196
BsaAI YACGTR 1 cut(s) 1053
BsaJI CCNNGG 8 cut(s) 286, 756, 1522, 1523, 1586, 1927, 2004, 2098
BsaWI WCCGGW 1 cut(s) 911
BsaXI ACNNNNNCTCC 2 cut(s) 2001, 2031
Bsc4I CCNNNNNNNGG 7 cut(s) 199, 287, 911, 1079, 1405, 1927, 2159
Bse118I RCCGGY 2 cut(s) 75, 911
Bse1I ACTGG 4 cut(s) 1597, 1777, 2228, 2459
Bse3DI GCAATG 3 cut(s) 1575, 1978, 2070
BseBI CCWGG 7 cut(s) 288, 1460, 1488, 1524, 1587, 1928, 2005
BseDI CCNNGG 8 cut(s) 286, 756, 1522, 1523, 1586, 1927, 2004, 2098
BseGI GGATG 4 cut(s) 464, 1198, 1648, 2370
BseLI CCNNNNNNNGG 7 cut(s) 199, 287, 911, 1079, 1405, 1927, 2159
BseMI GCAATG 3 cut(s) 1575, 1978, 2070
BseMII CTCAG 9 cut(s) 248, 309, 342, 1019, 1319, 1365, 1611, 1822, 2202
BseNI ACTGG 4 cut(s) 1597, 1777, 2228, 2459
BseRI GAGGAG 2 cut(s) 438, 855
BseXI GCAGC 6 cut(s) 29, 604, 1567, 1961, 2187, 2297
BseYI CCCAGC 1 cut(s) 521
BsgI GTGCAG 3 cut(s) 1208, 1566, 2296
BshFI GGCC 6 cut(s) 338, 395, 635, 882, 1274, 2107
BshNI GGYRCC 2 cut(s) 78, 1082
BshTI ACCGGT 1 cut(s) 911
BsiHKCI CYCGRG 1 cut(s) 661
BsiSI CCGG 2 cut(s) 76, 912
BslFI GGGAC 4 cut(s) 237, 365, 2240, 2488
BslI CCNNNNNNNGG 7 cut(s) 199, 287, 911, 1079, 1405, 1927, 2159
BsmAI GTCTC 2 cut(s) 732, 1642
BsmFI GGGAC 4 cut(s) 237, 365, 2240, 2488
BsmI GAATGC 3 cut(s) 1432, 1900, 2270
BsnI GGCC 6 cut(s) 338, 395, 635, 882, 1274, 2107
BsoBI CYCGRG 1 cut(s) 661
BspACI CCGC 4 cut(s) 1509, 1969, 1997, 2243
BspANI GGCC 6 cut(s) 338, 395, 635, 882, 1274, 2107
BspCNI CTCAG 9 cut(s) 247, 310, 341, 1018, 1318, 1364, 1612, 1821, 2203
BspHI TCATGA 1 cut(s) 504
BspLI GGNNCC 7 cut(s) 80, 1048, 1084, 1110, 1133, 1967, 2527
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 2032
BspOI GCTAGC 1 cut(s) 521
BspPI GGATC 7 cut(s) 330, 747, 794, 945, 1556, 2138, 2144
BspT107I GGYRCC 2 cut(s) 78, 1082
BsrDI GCAATG 3 cut(s) 1575, 1978, 2070
BsrFI RCCGGY 2 cut(s) 75, 911
BsrI ACTGG 4 cut(s) 1597, 1777, 2228, 2459
BssAI RCCGGY 2 cut(s) 75, 911
BssECI CCNNGG 8 cut(s) 286, 756, 1522, 1523, 1586, 1927, 2004, 2098
BssT1I CCWWGG 2 cut(s) 756, 2098
Bst2UI CCWGG 7 cut(s) 288, 1460, 1488, 1524, 1587, 1928, 2005
Bst4CI ACNGT 7 cut(s) 346, 1671, 1759, 1988, 2053, 2072, 2502
Bst6I CTCTTC 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 1316
BstBAI YACGTR 1 cut(s) 1053
BstC8I GCNNGC 7 cut(s) 53, 519, 830, 993, 1450, 2109, 2173
BstF5I GGATG 4 cut(s) 464, 1198, 1648, 2370
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 1 cut(s) 317
BstMAI GTCTC 2 cut(s) 732, 1642
BstMWI GCNNNNNNNGC 7 cut(s) 17, 879, 988, 1186, 1316, 1589, 2493
BstNI CCWGG 7 cut(s) 288, 1460, 1488, 1524, 1587, 1928, 2005
BstNSI RCATGY 3 cut(s) 55, 263, 1325
BstSCI CCNGG 7 cut(s) 286, 1458, 1486, 1522, 1585, 1926, 2003
BstSFI CTRYAG 2 cut(s) 15, 1197
BstV1I GCAGC 6 cut(s) 29, 604, 1567, 1961, 2187, 2297
BstV2I GAAGAC 2 cut(s) 1340, 1353
BstX2I RGATCY 4 cut(s) 322, 752, 799, 2130
BstYI RGATCY 4 cut(s) 322, 752, 799, 2130
BsuRI GGCC 6 cut(s) 338, 395, 635, 882, 1274, 2107
BtsCI GGATG 4 cut(s) 464, 1198, 1648, 2370
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 6 cut(s) 566, 612, 1254, 1617, 1755, 1904
BveI ACCTGC 1 cut(s) 2032
Cac8I GCNNGC 7 cut(s) 53, 519, 830, 993, 1450, 2109, 2173
CciI TCATGA 1 cut(s) 504
CfoI GCGC 1 cut(s) 317
Cfr10I RCCGGY 2 cut(s) 75, 911
Cfr13I GGNCC 3 cut(s) 393, 1132, 2105
CseI GACGC 2 cut(s) 68, 2502
Csp6I GTAC 5 cut(s) 79, 209, 785, 1090, 1918
CspAI ACCGGT 1 cut(s) 911
CviQI GTAC 5 cut(s) 79, 209, 785, 1090, 1918
DrdI GACNNNNNNGTC 1 cut(s) 1601
DseDI GACNNNNNNGTC 1 cut(s) 1601
EaeI YGGCCR 2 cut(s) 633, 880
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Eco130I CCWWGG 2 cut(s) 756, 2098
Eco32I GATATC 1 cut(s) 1162
Eco47I GGWCC 1 cut(s) 1132
Eco47III AGCGCT 1 cut(s) 316
Eco57I CTGAAG 3 cut(s) 122, 611, 1056
Eco72I CACGTG 1 cut(s) 1053
Eco88I CYCGRG 1 cut(s) 661
EcoO109I RGGNCCY 1 cut(s) 1132
EcoRI GAATTC 1 cut(s) 535
EcoRII CCWGG 7 cut(s) 286, 1458, 1486, 1522, 1585, 1926, 2003
EcoRV GATATC 1 cut(s) 1162
EcoT14I CCWWGG 2 cut(s) 756, 2098
EcoT22I ATGCAT 1 cut(s) 1902
ErhI CCWWGG 2 cut(s) 756, 2098
FalI AAGNNNNNCTT 4 cut(s) 360, 392, 1373, 1405
FaqI GGGAC 4 cut(s) 237, 365, 2240, 2488
FauI CCCGC 1 cut(s) 2236
FauNDI CATATG 2 cut(s) 84, 1698
FbaI TGATCA 1 cut(s) 594
Fnu4HI GCNGC 6 cut(s) 18, 618, 1581, 1950, 2176, 2311
FokI GGATG 4 cut(s) 451, 1205, 1635, 2357
Fsp4HI GCNGC 6 cut(s) 18, 618, 1581, 1950, 2176, 2311
FspBI CTAG 2 cut(s) 518, 2490
GlaI GCGC 1 cut(s) 316
GluI GCNGC 6 cut(s) 18, 618, 1581, 1950, 2176, 2311
GsaI CCCAGC 1 cut(s) 525
GsuI CTGGAG 3 cut(s) 780, 1910, 2347
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 6 cut(s) 338, 395, 635, 882, 1274, 2107
HapII CCGG 2 cut(s) 76, 912
HgaI GACGC 2 cut(s) 68, 2502
HhaI GCGC 1 cut(s) 317
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 1 cut(s) 1822
HindII GTYRAC 1 cut(s) 1822
HindIII AAGCTT 2 cut(s) 623, 2037
HinfI GANTC 5 cut(s) 5, 35, 1026, 1349, 1635
HpaI GTTAAC 1 cut(s) 1822
HpaII CCGG 2 cut(s) 76, 912
HphI GGTGA 4 cut(s) 334, 2174, 2419, 2455
Hpy166II GTNNAC 5 cut(s) 553, 655, 917, 1822, 2078
Hpy8I GTNNAC 5 cut(s) 553, 655, 917, 1822, 2078
Hpy99I CGWCG 2 cut(s) 62, 1143
HpyAV CCTTC 5 cut(s) 667, 721, 1145, 1360, 1863
HpyCH4III ACNGT 7 cut(s) 346, 1671, 1759, 1988, 2053, 2072, 2502
HpyCH4IV ACGT 1 cut(s) 1052
HpyF10VI GCNNNNNNNGC 7 cut(s) 17, 879, 988, 1186, 1316, 1589, 2493
HpySE526I ACGT 1 cut(s) 1052
HspAI GCGC 1 cut(s) 315
KpnI GGTACC 1 cut(s) 82
Ksp22I TGATCA 1 cut(s) 594
KspAI GTTAAC 1 cut(s) 1822
LmnI GCTCC 5 cut(s) 799, 972, 1929, 2009, 2525
Lsp1109I GCAGC 6 cut(s) 29, 604, 1567, 1961, 2187, 2297
LweI GCATC 5 cut(s) 504, 1183, 1557, 1887, 2158
MaeI CTAG 2 cut(s) 518, 2490
MaeII ACGT 1 cut(s) 1052
MaeIII GTNAC 4 cut(s) 643, 1018, 2429, 2461
MfeI CAATTG 2 cut(s) 432, 1314
MflI RGATCY 4 cut(s) 322, 752, 799, 2130
MlsI TGGCCA 2 cut(s) 635, 882
MluNI TGGCCA 2 cut(s) 635, 882
MlyI GAGTC 4 cut(s) 29, 1020, 1343, 1644
MmeI TCCRAC 2 cut(s) 1816, 2044
Mox20I TGGCCA 2 cut(s) 635, 882
Mph1103I ATGCAT 1 cut(s) 1902
MscI TGGCCA 2 cut(s) 635, 882
MslI CAYNNNNRTG 3 cut(s) 344, 641, 2375
Msp20I TGGCCA 2 cut(s) 635, 882
MspA1I CMGCKG 1 cut(s) 2111
MspI CCGG 2 cut(s) 76, 912
MspR9I CCNGG 7 cut(s) 288, 1460, 1488, 1524, 1587, 1928, 2005
MunI CAATTG 2 cut(s) 432, 1314
Mva1269I GAATGC 3 cut(s) 1432, 1900, 2270
MvaI CCWGG 7 cut(s) 288, 1460, 1488, 1524, 1587, 1928, 2005
MwoI GCNNNNNNNGC 7 cut(s) 17, 879, 988, 1186, 1316, 1589, 2493
NdeI CATATG 2 cut(s) 84, 1698
NheI GCTAGC 1 cut(s) 517
NlaIV GGNNCC 7 cut(s) 80, 1048, 1084, 1110, 1133, 1967, 2527
NmuCI GTSAC 3 cut(s) 643, 2429, 2461
NsiI ATGCAT 1 cut(s) 1902
NspI RCATGY 3 cut(s) 55, 263, 1325
PaeI GCATGC 1 cut(s) 55
PagI TCATGA 1 cut(s) 504
PasI CCCWGGG 1 cut(s) 1523
PciI ACATGT 1 cut(s) 259
PcsI WCGNNNNNNNCGW 1 cut(s) 668
PctI GAATGC 3 cut(s) 1432, 1900, 2270
PfeI GAWTC 1 cut(s) 5
PflMI CCANNNNNTGG 3 cut(s) 199, 287, 1405
PfoI TCCNGGA 1 cut(s) 1458
PinAI ACCGGT 1 cut(s) 911
PkrI GCNGC 6 cut(s) 19, 619, 1582, 1951, 2177, 2312
PleI GAGTC 4 cut(s) 29, 1020, 1343, 1643
PmaCI CACGTG 1 cut(s) 1053
PmlI CACGTG 1 cut(s) 1053
PpsI GAGTC 4 cut(s) 29, 1020, 1343, 1643
Ppu21I YACGTR 1 cut(s) 1053
PpuMI RGGWCCY 1 cut(s) 1132
PscI ACATGT 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 1132
Psp6I CCWGG 7 cut(s) 286, 1458, 1486, 1522, 1585, 1926, 2003
PspCI CACGTG 1 cut(s) 1053
PspFI CCCAGC 1 cut(s) 521
PspGI CCWGG 7 cut(s) 286, 1458, 1486, 1522, 1585, 1926, 2003
PspN4I GGNNCC 7 cut(s) 80, 1048, 1084, 1110, 1133, 1967, 2527
PspPI GGNCC 3 cut(s) 393, 1132, 2105
PspPPI RGGWCCY 1 cut(s) 1132
PstI CTGCAG 1 cut(s) 19
PsuI RGATCY 4 cut(s) 322, 752, 799, 2130
PvuII CAGCTG 1 cut(s) 2111
RsaI GTAC 5 cut(s) 80, 210, 786, 1091, 1919
RsaNI GTAC 5 cut(s) 79, 209, 785, 1090, 1918
RseI CAYNNNNRTG 3 cut(s) 344, 641, 2375
SatI GCNGC 6 cut(s) 18, 618, 1581, 1950, 2176, 2311
Sau96I GGNCC 3 cut(s) 393, 1132, 2105
ScaI AGTACT 3 cut(s) 210, 786, 1091
SchI GAGTC 4 cut(s) 29, 1020, 1343, 1644
ScrFI CCNGG 7 cut(s) 288, 1460, 1488, 1524, 1587, 1928, 2005
SfaNI GCATC 5 cut(s) 504, 1183, 1557, 1887, 2158
SfcI CTRYAG 2 cut(s) 15, 1197
SgrAI CRCCGGYG 1 cut(s) 911
SinI GGWCC 1 cut(s) 1132
SmiMI CAYNNNNRTG 3 cut(s) 344, 641, 2375
SmlI CTYRAG 1 cut(s) 1175
SmoI CTYRAG 1 cut(s) 1175
SphI GCATGC 1 cut(s) 55
SsiI CCGC 4 cut(s) 1509, 1969, 1997, 2243
SspI AATATT 1 cut(s) 858
SspMI CTAG 2 cut(s) 518, 2490
StyD4I CCNGG 7 cut(s) 286, 1458, 1486, 1522, 1585, 1926, 2003
StyI CCWWGG 2 cut(s) 756, 2098
TaaI ACNGT 7 cut(s) 346, 1671, 1759, 1988, 2053, 2072, 2502
TaiI ACGT 1 cut(s) 1055
TaqI TCGA 5 cut(s) 470, 512, 1212, 1421, 1560
TatI WGTACW 3 cut(s) 208, 784, 1089
TfiI GAWTC 1 cut(s) 5
TscAI CASTG 6 cut(s) 573, 619, 1261, 1624, 1762, 1911
TseFI GTSAC 3 cut(s) 643, 2429, 2461
TseI GCWGC 6 cut(s) 17, 617, 1580, 1949, 2175, 2310
Tsp45I GTSAC 3 cut(s) 643, 2429, 2461
TspRI CASTG 6 cut(s) 573, 619, 1261, 1624, 1762, 1911
Van91I CCANNNNNTGG 3 cut(s) 199, 287, 1405
VpaK11BI GGWCC 1 cut(s) 1132
XapI RAATTY 6 cut(s) 299, 535, 691, 1264, 1568, 2401
XceI RCATGY 3 cut(s) 55, 263, 1325
XspI CTAG 2 cut(s) 518, 2490
ZrmI AGTACT 3 cut(s) 210, 786, 1091
Zsp2I ATGCAT 1 cut(s) 1902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.