AT5G38520

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015325
Physical Location & Seq
Forward (+)
15421289 .. 15423431
2143 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G38520.2

Sequence Viewer

Length: 1089 bp
ATGAGAGCTCTAACATGGACGGCAATGTCGCCGCCGGTGATGTCACGGACGGCGACTTCTACGGTTAACTTACGACGTATCAGCCTACGGAGAGATCGCGTCTGTGTTAGAGCCACGGCTTCGTCTAGCGCCACGGTTTCCGGCGGAGGAGTAGTAGAGGCGGTAGAATTGGCAGAGATAGGAGAAAGAAGCAAGAAATGGAAGTGGAAAGGAGAATATTCTGTTAACTACTTTGTCAAAGATTCGCCGGAGGAAGTTACTCCGGCGAGTCAAACTGTTCTTTTGGTTCATGGCTTTGGTGCTTCTATTCCTCACTGGCGAAGGAACATAAATGCTTTGTCTAAAAACCATACAGTGTATGCAATTGATCTTCTCGGGTTTGGTGCTTCGGATAAGCCACCTGGTTTTAGCTATACCATGGAGTCATGGGCTGAGTTGATACTCAACTTCTTGGAGGAAGTGGTTCAGAAACCGACTATTTTGATTGGAAACTCTGTTGGAAGCCTTGCTTGTGTCATCGCTGCCTCAGAATCACGAGGAGATCTGGTCAAAGGTCTTGTTCTATTGAATTGTGCTGGTGGTATGAACAACAAAGCTGTCTTTGATGACTGGAGAATCAAGCTACTGATGCCTTTACTCTTACTTATCGACTTCTTACTCAAGCAAAGAGGAATTGCTTCTGCACTCTTCAACCGTGTTAAAGACAGGGAAAATCTGAAGAACATCTTGACAAATGTTTACGGGAACAAGGACAATGTAGATGACACCCTTGTAGAGATCATTGCTGGACCAGCAAATACCGAAGGTGCACTAGATGCTTTTGTTTCAATCTTAACGGGTCCTCCTGGACCAAATCCGATTAAGCTGATACCGGAAATAACCAAACCGGTTCTTGTCTTATGGGGAGATCAAGATGGACTAACCCCTCTTGATGGTCCAGTAGGTAAGTACTTCACTTCCCTTCCGGATCAGTTACCTAACTTCAACCTCTATGTTCTACAAGGCGTTGGACATTGCCCGCAAGATGATCGTCCGGATCTTGTCCATGAGCGTCTCCTTCCATGGCTGGCTCAACTTTCTTCCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

362

Amino Acids

39.57

Weight (kDa)

6.54

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 89 - 175 1.3e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 92 - 357 1.5e-22 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 93 - 361 1.6e-20 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38520 AT5G38520
fragaria_vesca FvH4_2g37190
malus_domestica MD08G1001000.v1.1
prunus_persica Prupe.1G353100_v2.0.a1
pyrus_communis pycom08g00040
rosa_chinensis RchiOBHm_Chr6g0304501
rosa_laevigata RLG00000010971
rosa_multiflora Rmu_sc0023263.1_g000001 Rmu_ssc0000218.1_g000058
rosa_roxburghii Rroxscaffold_7G00163690
rosa_rugosa Rorug06G0332600
rosa_samantha Rh6AG445100 Rh6CG457900 Rh6DG444700
rosa_wichuraiana Rw6G038640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 25
AccII CGCG 1 cut(s) 99
AccIII TCCGGA 2 cut(s) 964, 1033
AciI CCGC 4 cut(s) 32, 144, 161, 1019
AclWI GGATC 2 cut(s) 975, 1044
AcuI CTGAAG 1 cut(s) 737
AfaI GTAC 1 cut(s) 950
AfiI CCNNNNNNNGG 1 cut(s) 932
AgeI ACCGGT 1 cut(s) 886
AgsI TTSAA 4 cut(s) 568, 691, 828, 985
AjnI CCWGG 2 cut(s) 400, 844
AluBI AGCT 5 cut(s) 8, 411, 596, 622, 865
AluI AGCT 5 cut(s) 8, 411, 596, 622, 865
Alw21I GWGCWC 2 cut(s) 10, 811
Alw26I GTCTC 1 cut(s) 1058
Alw44I GTGCAC 1 cut(s) 807
AlwI GGATC 2 cut(s) 975, 1044
Ama87I CYCGRG 1 cut(s) 374
Aor13HI TCCGGA 2 cut(s) 964, 1033
ApaLI GTGCAC 1 cut(s) 807
ApeKI GCWGC 1 cut(s) 521
AsiGI ACCGGT 1 cut(s) 886
Asp700I GAANNNNTTC 2 cut(s) 462, 676
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 4 cut(s) 788, 839, 848, 935
AsuHPI GGTGA 1 cut(s) 49
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 4 cut(s) 788, 839, 848, 935
BaeGI GKGCMC 1 cut(s) 811
BanII GRGCYC 1 cut(s) 10
BauI CACGAG 1 cut(s) 534
Bbv12I GWGCWC 2 cut(s) 10, 811
BbvI GCAGC 1 cut(s) 508
BccI CCATC 2 cut(s) 908, 926
BceAI ACGGC 3 cut(s) 36, 66, 132
BcgI CGANNNNNNTGC 2 cut(s) 1010, 1044
BciT130I CCWGG 2 cut(s) 402, 846
BcoDI GTCTC 1 cut(s) 1058
BfaI CTAG 3 cut(s) 126, 812, 1087
BfoI RGCGCY 1 cut(s) 132
BglII AGATCT 1 cut(s) 541
BisI GCNGC 2 cut(s) 32, 522
BlsI GCNGC 2 cut(s) 33, 523
BmcAI AGTACT 1 cut(s) 950
Bme1390I CCNGG 2 cut(s) 402, 846
Bme18I GGWCC 4 cut(s) 788, 839, 848, 935
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 4 cut(s) 788, 839, 848, 935
BmiI GGNNCC 1 cut(s) 840
BmrFI CCNGG 2 cut(s) 402, 846
BmsI GCATC 2 cut(s) 618, 805
BpmI CTGGAG 1 cut(s) 631
BpuEI CTTGAG 1 cut(s) 644
BsaJI CCNNGG 4 cut(s) 114, 132, 417, 1061
BsaWI WCCGGW 4 cut(s) 871, 886, 964, 1033
BsaXI ACNNNNNCTCC 6 cut(s) 138, 168, 531, 561, 826, 856
Bsc4I CCNNNNNNNGG 1 cut(s) 932
Bse118I RCCGGY 2 cut(s) 34, 886
Bse1I ACTGG 3 cut(s) 320, 614, 938
Bse3DI GCAATG 3 cut(s) 30, 780, 1012
BseAI TCCGGA 2 cut(s) 964, 1033
BseBI CCWGG 2 cut(s) 402, 846
BseDI CCNNGG 4 cut(s) 114, 132, 417, 1061
BseLI CCNNNNNNNGG 1 cut(s) 932
BseMI GCAATG 3 cut(s) 30, 780, 1012
BseMII CTCAG 2 cut(s) 423, 540
BseNI ACTGG 3 cut(s) 320, 614, 938
BseRI GAGGAG 2 cut(s) 162, 552
BseSI GKGCMC 1 cut(s) 811
BseXI GCAGC 1 cut(s) 508
BsgI GTGCAG 1 cut(s) 666
Bsh1236I CGCG 1 cut(s) 99
BshTI ACCGGT 1 cut(s) 886
BsiHKAI GWGCWC 2 cut(s) 10, 811
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 8 cut(s) 35, 141, 248, 263, 872, 887, 965, 1034
BslI CCNNNNNNNGG 1 cut(s) 932
BsmAI GTCTC 1 cut(s) 1058
BsmBI CGTCTC 1 cut(s) 1058
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 2 cut(s) 10, 811
Bsp13I TCCGGA 2 cut(s) 964, 1033
Bsp143I GATC 8 cut(s) 94, 367, 541, 777, 907, 967, 1027, 1036
Bsp19I CCATGG 2 cut(s) 417, 1061
BspACI CCGC 4 cut(s) 32, 144, 161, 1019
BspCNI CTCAG 2 cut(s) 424, 539
BspEI TCCGGA 2 cut(s) 964, 1033
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 840
BspPI GGATC 2 cut(s) 975, 1044
BsrDI GCAATG 3 cut(s) 30, 780, 1012
BsrFI RCCGGY 2 cut(s) 34, 886
BsrI ACTGG 3 cut(s) 320, 614, 938
BssAI RCCGGY 2 cut(s) 34, 886
BssECI CCNNGG 4 cut(s) 114, 132, 417, 1061
BssMI GATC 8 cut(s) 94, 367, 541, 777, 907, 967, 1027, 1036
BssSI CACGAG 1 cut(s) 534
BssT1I CCWWGG 2 cut(s) 417, 1061
Bst2BI CACGAG 1 cut(s) 534
Bst2UI CCWGG 2 cut(s) 402, 846
Bst4CI ACNGT 5 cut(s) 64, 136, 277, 355, 695
Bst6I CTCTTC 1 cut(s) 692
BstAPI GCANNNNNTGC 1 cut(s) 815
BstC8I GCNNGC 2 cut(s) 1019, 1068
BstDEI CTNAG 2 cut(s) 432, 526
BstDSI CCRYGG 4 cut(s) 114, 132, 417, 1061
BstFNI CGCG 1 cut(s) 99
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 8 cut(s) 97, 370, 544, 780, 910, 970, 1030, 1039
BstMAI GTCTC 1 cut(s) 1058
BstMBI GATC 8 cut(s) 94, 367, 541, 777, 907, 967, 1027, 1036
BstMWI GCNNNNNNNGC 3 cut(s) 628, 791, 815
BstNI CCWGG 2 cut(s) 402, 846
BstSCI CCNGG 2 cut(s) 400, 844
BstSLI GKGCMC 1 cut(s) 811
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 1 cut(s) 508
BstX2I RGATCY 2 cut(s) 541, 1036
BstYI RGATCY 2 cut(s) 541, 1036
BtgI CCRYGG 4 cut(s) 114, 132, 417, 1061
BtgZI GCGATG 1 cut(s) 502
BtsIMutI CAGTG 2 cut(s) 313, 360
Cac8I GCNNGC 2 cut(s) 1019, 1068
CfoI GCGC 1 cut(s) 131
Cfr10I RCCGGY 2 cut(s) 34, 886
Cfr13I GGNCC 4 cut(s) 788, 839, 848, 935
CseI GACGC 2 cut(s) 88, 1040
CsiI ACCWGGT 1 cut(s) 400
Csp6I GTAC 1 cut(s) 949
CspAI ACCGGT 1 cut(s) 886
CviAII CATG 6 cut(s) 15, 290, 418, 426, 1046, 1062
CviQI GTAC 1 cut(s) 949
DdeI CTNAG 2 cut(s) 432, 526
DpnI GATC 8 cut(s) 96, 369, 543, 779, 909, 969, 1029, 1038
DpnII GATC 8 cut(s) 94, 367, 541, 777, 907, 967, 1027, 1036
DrdI GACNNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 25
Eam1104I CTCTTC 1 cut(s) 692
EarI CTCTTC 1 cut(s) 692
EciI GGCGGA 1 cut(s) 159
Ecl136II GAGCTC 1 cut(s) 8
Eco130I CCWWGG 2 cut(s) 417, 1061
Eco24I GRGCYC 1 cut(s) 10
Eco47I GGWCC 4 cut(s) 788, 839, 848, 935
Eco53kI GAGCTC 1 cut(s) 8
Eco57I CTGAAG 1 cut(s) 737
Eco88I CYCGRG 1 cut(s) 374
EcoICRI GAGCTC 1 cut(s) 8
EcoO109I RGGNCCY 1 cut(s) 839
EcoRII CCWGG 2 cut(s) 400, 844
EcoT14I CCWWGG 2 cut(s) 417, 1061
EcoT38I GRGCYC 1 cut(s) 10
ErhI CCWWGG 2 cut(s) 417, 1061
Esp3I CGTCTC 1 cut(s) 1058
FaeI CATG 6 cut(s) 18, 293, 421, 429, 1049, 1065
FalI AAGNNNNNCTT 4 cut(s) 493, 525, 710, 742
FatI CATG 6 cut(s) 14, 289, 417, 425, 1045, 1061
FauI CCCGC 1 cut(s) 1026
Fnu4HI GCNGC 2 cut(s) 32, 522
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 2 cut(s) 32, 522
FspBI CTAG 3 cut(s) 126, 812, 1087
GlaI GCGC 1 cut(s) 130
GluI GCNGC 2 cut(s) 32, 522
GsuI CTGGAG 1 cut(s) 631
HaeII RGCGCY 1 cut(s) 132
HapII CCGG 8 cut(s) 35, 141, 248, 263, 872, 887, 965, 1034
HgaI GACGC 2 cut(s) 88, 1040
HhaI GCGC 1 cut(s) 131
Hin1II CATG 6 cut(s) 18, 293, 421, 429, 1049, 1065
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 2 cut(s) 67, 226
HindII GTYRAC 2 cut(s) 67, 226
HinfI GANTC 5 cut(s) 242, 268, 422, 530, 615
HpaI GTTAAC 2 cut(s) 67, 226
HpaII CCGG 8 cut(s) 35, 141, 248, 263, 872, 887, 965, 1034
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 4 cut(s) 67, 226, 739, 809
Hpy188I TCNGA 5 cut(s) 391, 468, 529, 717, 858
Hpy188III TCNNGA 6 cut(s) 534, 727, 911, 929, 965, 1034
Hpy8I GTNNAC 4 cut(s) 67, 226, 739, 809
Hpy99I CGWCG 1 cut(s) 78
HpyAV CCTTC 4 cut(s) 315, 797, 971, 1067
HpyCH4III ACNGT 5 cut(s) 64, 136, 277, 355, 695
HpyCH4IV ACGT 1 cut(s) 76
HpyCH4V TGCA 3 cut(s) 362, 683, 809
HpyF10VI GCNNNNNNNGC 3 cut(s) 628, 791, 815
HpyF3I CTNAG 2 cut(s) 432, 526
HpySE526I ACGT 1 cut(s) 76
Hsp92II CATG 6 cut(s) 18, 293, 421, 429, 1049, 1065
HspAI GCGC 1 cut(s) 129
Kpn2I TCCGGA 2 cut(s) 964, 1033
KspAI GTTAAC 2 cut(s) 67, 226
Kzo9I GATC 8 cut(s) 94, 367, 541, 777, 907, 967, 1027, 1036
Lsp1109I GCAGC 1 cut(s) 508
LweI GCATC 2 cut(s) 618, 805
MabI ACCWGGT 1 cut(s) 400
MaeI CTAG 3 cut(s) 126, 812, 1087
MaeII ACGT 1 cut(s) 76
MaeIII GTNAC 3 cut(s) 42, 256, 972
MalI GATC 8 cut(s) 96, 369, 543, 779, 909, 969, 1029, 1038
MboI GATC 8 cut(s) 94, 367, 541, 777, 907, 967, 1027, 1036
MboII GAAGA 4 cut(s) 362, 679, 730, 1071
MfeI CAATTG 1 cut(s) 363
MflI RGATCY 2 cut(s) 541, 1036
MhlI GDGCHC 2 cut(s) 10, 811
MluCI AATT 4 cut(s) 167, 363, 568, 672
MlyI GAGTC 2 cut(s) 277, 431
MmeI TCCRAC 2 cut(s) 478, 988
MroI TCCGGA 2 cut(s) 964, 1033
MroXI GAANNNNTTC 2 cut(s) 462, 676
MseI TTAA 5 cut(s) 66, 225, 699, 833, 861
MspI CCGG 8 cut(s) 35, 141, 248, 263, 872, 887, 965, 1034
MspR9I CCNGG 2 cut(s) 402, 846
MunI CAATTG 1 cut(s) 363
MvaI CCWGG 2 cut(s) 402, 846
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 3 cut(s) 628, 791, 815
NcoI CCATGG 2 cut(s) 417, 1061
NdeII GATC 8 cut(s) 94, 367, 541, 777, 907, 967, 1027, 1036
NlaIII CATG 6 cut(s) 18, 293, 421, 429, 1049, 1065
NlaIV GGNNCC 1 cut(s) 840
NmuCI GTSAC 1 cut(s) 42
PdmI GAANNNNTTC 2 cut(s) 462, 676
PfeI GAWTC 3 cut(s) 242, 530, 615
PfoI TCCNGGA 1 cut(s) 844
PinAI ACCGGT 1 cut(s) 886
PkrI GCNGC 2 cut(s) 33, 523
PleI GAGTC 2 cut(s) 276, 430
PpsI GAGTC 2 cut(s) 276, 430
PpuMI RGGWCCY 1 cut(s) 839
Psp124BI GAGCTC 1 cut(s) 10
Psp5II RGGWCCY 1 cut(s) 839
Psp6I CCWGG 2 cut(s) 400, 844
PspGI CCWGG 2 cut(s) 400, 844
PspN4I GGNNCC 1 cut(s) 840
PspPI GGNCC 4 cut(s) 788, 839, 848, 935
PspPPI RGGWCCY 1 cut(s) 839
PsuI RGATCY 2 cut(s) 541, 1036
RsaI GTAC 1 cut(s) 950
RsaNI GTAC 1 cut(s) 949
SacI GAGCTC 1 cut(s) 10
SaqAI TTAA 5 cut(s) 66, 225, 699, 833, 861
SatI GCNGC 2 cut(s) 32, 522
Sau3AI GATC 8 cut(s) 94, 367, 541, 777, 907, 967, 1027, 1036
Sau96I GGNCC 4 cut(s) 788, 839, 848, 935
ScaI AGTACT 1 cut(s) 950
SchI GAGTC 2 cut(s) 277, 431
ScrFI CCNGG 2 cut(s) 402, 846
SduI GDGCHC 2 cut(s) 10, 811
SexAI ACCWGGT 1 cut(s) 400
SfaNI GCATC 2 cut(s) 618, 805
SgrAI CRCCGGYG 1 cut(s) 34
SinI GGWCC 4 cut(s) 788, 839, 848, 935
SmlI CTYRAG 1 cut(s) 659
SmoI CTYRAG 1 cut(s) 659
Sse9I AATT 4 cut(s) 167, 363, 568, 672
SsiI CCGC 4 cut(s) 32, 144, 161, 1019
SspI AATATT 1 cut(s) 218
SspMI CTAG 3 cut(s) 126, 812, 1087
SstI GAGCTC 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 400, 844
StyI CCWWGG 2 cut(s) 417, 1061
TaaI ACNGT 5 cut(s) 64, 136, 277, 355, 695
TaiI ACGT 1 cut(s) 79
TaqI TCGA 1 cut(s) 648
TasI AATT 4 cut(s) 167, 363, 568, 672
TatI WGTACW 1 cut(s) 948
TauI GCSGC 1 cut(s) 34
TfiI GAWTC 3 cut(s) 242, 530, 615
Tru1I TTAA 5 cut(s) 66, 225, 699, 833, 861
Tru9I TTAA 5 cut(s) 66, 225, 699, 833, 861
TscAI CASTG 2 cut(s) 320, 360
TseFI GTSAC 1 cut(s) 42
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 1 cut(s) 42
TspDTI ATGAA 2 cut(s) 278, 599
TspGWI ACGGA 2 cut(s) 61, 103
TspRI CASTG 2 cut(s) 320, 360
VneI GTGCAC 1 cut(s) 807
VpaK11BI GGWCC 4 cut(s) 788, 839, 848, 935
XmnI GAANNNNTTC 2 cut(s) 462, 676
XspI CTAG 3 cut(s) 126, 812, 1087
ZrmI AGTACT 1 cut(s) 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.