FvH4_2g37190

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015325
Physical Location & Seq
Forward (+)
27144948 .. 27147391
2444 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g37190.t1

Sequence Viewer

Length: 1233 bp
ATGGGGGTGACGGTAATATTGTGTCCCCATTCACTGCCAAAATTTTTGCATTTACAGCCCTCGACACCAAACCAGAAAGCAGTAAAAACTAGTTGTTTGCTTAGCTATGGTGGACGACGAGCAGGTGAAGGAGGAGTCTTGCTCCGTCAAAATAACCAACCAAACACGACCACCATCAAGAGTAGTTGTATCACTACTCTTCGATCCATGAAACTCAACTCTTCATCTTCATCTTCATCATCTAGAACTGCAGCAAAGGCAACTAGTGCTGGTGCCGCCGCAGCCGCCGACGATGTAGTGTTGGAATTGGAGCTGGGAGAGATCAAGGACAAGTACATGAAGTGGAACTGGAAGGGGCAGTACGCCATCAACTACGTCGTTTCACATCCAGAGAACGCAAATCCGAATCCAAATACTCCTCCTCTTCTTCTTGTTCATGGCTTCGGCGCCTCCATTCCTCACTGGCGCAGAAATATTAAGACATTGGCCAAGAATTATACCGTTTATGCTATTGACCTGATCGGGTTTGGTGCCTCAGATAAGCCGATAGGCTTTTCATACACCATGGAGAAATGGGCTGAGTTAATCTTGGATTTCTTGGATGAAGTTGTTCAGAAGCCTACTGTTTTGATAGGCAACTCTGTGGGAAGCCTTGCATGTGTAATTTCTGCCTCAGAATCTAAGCAAGGACTGGTGCGAGGCCTAGTGCTTTTAAATTGCTCTGGTGGGATGAATAACAAGGCCATTGTTGATGATTGGAGGATCAAGCTGATCTTCCCTTTGCTCTTGCTAATCGACTTCTTGTTGCAGCAACGACCTATTGCTTCCTTCATCTTTGAACGTGTTAGACAGAGAGAAAGTTTAAGAAATATCTTATCGTCGGTATATGCTAACAAAGAATCCGTGGATGACGAATTAGTTGAGATCATCATGGAACCAACAAATGACCCGGGTGCATTGGATGCCTTTGTCTCGATCGTCACAGGTCCACCGGGACCGAACCCGGTCCAGTTGATACCAGGCATCTCCCTACCCATTCTAGTCTTATGGGGCGATGAAGACCCGTTCACTCCTCTTGATGGGCCTGTTGGGAAATACTTCTCTTCCTTACCTTCTCAGCAATCCAATGTGAGCCTCTCCGTTTTGCAAGGAGTTGGGCATTGCCCCCACGATGACAGGCCCAAGTTGGTACACGACAGACTTCTTCCCTGGTTGGCGAATCTTTCTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

411

Amino Acids

44.64

Weight (kDa)

7.63

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 140 - 394 7.3e-25 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 142 - 399 1.2e-21 Alpha/beta hydrolase family
Hydrolase_4 PF12146 142 - 248 5.3e-09 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38520 AT5G38520
fragaria_vesca FvH4_2g37190
malus_domestica MD08G1001000.v1.1
prunus_persica Prupe.1G353100_v2.0.a1
pyrus_communis pycom08g00040
rosa_chinensis RchiOBHm_Chr6g0304501
rosa_laevigata RLG00000010971
rosa_multiflora Rmu_sc0023263.1_g000001 Rmu_ssc0000218.1_g000058
rosa_roxburghii Rroxscaffold_7G00163690
rosa_rugosa Rorug06G0332600
rosa_samantha Rh6AG445100 Rh6CG457900 Rh6DG444700
rosa_wichuraiana Rw6G038640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 113
Acc36I ACCTGC 1 cut(s) 113
AccB1I GGYRCC 3 cut(s) 272, 446, 530
AciI CCGC 3 cut(s) 276, 279, 285
AclWI GGATC 2 cut(s) 198, 770
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 1 cut(s) 41
AcyI GRCGYC 1 cut(s) 447
AfaI GTAC 3 cut(s) 335, 362, 1191
AfiI CCNNNNNNNGG 1 cut(s) 1079
AflIII ACRYGT 1 cut(s) 841
AgsI TTSAA 1 cut(s) 839
AhlI ACTAGT 2 cut(s) 89, 263
AjnI CCWGG 2 cut(s) 1018, 1208
AluBI AGCT 3 cut(s) 105, 313, 769
AluI AGCT 3 cut(s) 105, 313, 769
Alw26I GTCTC 1 cut(s) 976
AlwI GGATC 2 cut(s) 198, 770
Ama87I CYCGRG 1 cut(s) 949
AoxI GGCC 5 cut(s) 486, 700, 741, 1082, 1178
ApeKI GCWGC 3 cut(s) 251, 281, 808
ApoI RAATTY 1 cut(s) 41
Asp700I GAANNNNTTC 2 cut(s) 609, 1097
AspLEI GCGC 2 cut(s) 449, 468
AspS9I GGNCC 5 cut(s) 986, 995, 1006, 1082, 1179
AsuC2I CCSGG 4 cut(s) 950, 951, 993, 1004
AsuHPI GGTGA 2 cut(s) 19, 137
AvaI CYCGRG 1 cut(s) 949
AvaII GGWCC 3 cut(s) 986, 995, 1006
BalI TGGCCA 1 cut(s) 488
BanI GGYRCC 3 cut(s) 272, 446, 530
BarI GAAGNNNNNNTAC 2 cut(s) 344, 376
BbsI GAAGAC 1 cut(s) 1065
BbvI GCAGC 3 cut(s) 263, 293, 820
BccI CCATC 3 cut(s) 182, 374, 1073
BciT130I CCWGG 2 cut(s) 1020, 1210
BcnI CCSGG 4 cut(s) 950, 951, 993, 1004
BcoDI GTCTC 1 cut(s) 976
BcuI ACTAGT 2 cut(s) 89, 263
BfaI CTAG 5 cut(s) 90, 243, 264, 704, 1040
BfmI CTRYAG 1 cut(s) 249
BfoI RGCGCY 1 cut(s) 450
BfuAI ACCTGC 1 cut(s) 113
BisI GCNGC 6 cut(s) 252, 276, 279, 282, 285, 809
BlpI GCTNAGC 1 cut(s) 101
BlsI GCNGC 6 cut(s) 253, 277, 280, 283, 286, 810
Bme1390I CCNGG 6 cut(s) 950, 951, 993, 1004, 1020, 1210
Bme18I GGWCC 3 cut(s) 986, 995, 1006
BmeT110I CYCGRG 1 cut(s) 949
BmgT120I GGNCC 5 cut(s) 986, 995, 1006, 1082, 1179
BmiI GGNNCC 5 cut(s) 274, 448, 532, 936, 996
BmrFI CCNGG 6 cut(s) 950, 951, 993, 1004, 1020, 1210
BmsI GCATC 2 cut(s) 952, 1032
BpiI GAAGAC 1 cut(s) 1065
BplI GAGNNNNNCTC 2 cut(s) 126, 158
Bpu1102I GCTNAGC 1 cut(s) 101
BpuMI CCSGG 4 cut(s) 950, 951, 993, 1004
BsaHI GRCGYC 1 cut(s) 447
BsaJI CCNNGG 4 cut(s) 564, 903, 949, 1208
Bsc4I CCNNNNNNNGG 1 cut(s) 1079
Bse1I ACTGG 4 cut(s) 353, 467, 696, 1009
Bse3DI GCAATG 1 cut(s) 1159
BseBI CCWGG 2 cut(s) 1020, 1210
BseDI CCNNGG 4 cut(s) 564, 903, 949, 1208
BseGI GGATG 5 cut(s) 385, 607, 735, 913, 967
BseLI CCNNNNNNNGG 1 cut(s) 1079
BseMI GCAATG 1 cut(s) 1159
BseMII CTCAG 4 cut(s) 549, 570, 687, 1130
BseNI ACTGG 4 cut(s) 353, 467, 696, 1009
BseRI GAGGAG 4 cut(s) 147, 408, 411, 1062
BseXI GCAGC 3 cut(s) 263, 293, 820
BseYI CCCAGC 1 cut(s) 313
Bsh1285I CGRYCG 1 cut(s) 978
BshFI GGCC 5 cut(s) 488, 702, 743, 1084, 1180
BshNI GGYRCC 3 cut(s) 272, 446, 530
BsiEI CGRYCG 1 cut(s) 978
BsiHKCI CYCGRG 1 cut(s) 949
BsiSI CCGG 3 cut(s) 950, 992, 1004
BslFI GGGAC 2 cut(s) 9, 1008
BslI CCNNNNNNNGG 1 cut(s) 1079
BsmAI GTCTC 1 cut(s) 976
BsmFI GGGAC 2 cut(s) 9, 1008
BsnI GGCC 5 cut(s) 488, 702, 743, 1084, 1180
BsoBI CYCGRG 1 cut(s) 949
Bsp143I GATC 7 cut(s) 203, 321, 519, 762, 771, 924, 975
Bsp1720I GCTNAGC 1 cut(s) 101
Bsp19I CCATGG 1 cut(s) 564
BspACI CCGC 3 cut(s) 276, 279, 285
BspANI GGCC 5 cut(s) 488, 702, 743, 1084, 1180
BspCNI CTCAG 4 cut(s) 548, 571, 686, 1129
BspLI GGNNCC 5 cut(s) 274, 448, 532, 936, 996
BspMAI CTGCAG 1 cut(s) 253
BspMI ACCTGC 1 cut(s) 113
BspPI GGATC 2 cut(s) 198, 770
BspT107I GGYRCC 3 cut(s) 272, 446, 530
BsrDI GCAATG 1 cut(s) 1159
BsrI ACTGG 4 cut(s) 353, 467, 696, 1009
BssECI CCNNGG 4 cut(s) 564, 903, 949, 1208
BssMI GATC 7 cut(s) 203, 321, 519, 762, 771, 924, 975
BssNI GRCGYC 1 cut(s) 447
BssT1I CCWWGG 1 cut(s) 564
Bst2UI CCWGG 2 cut(s) 1020, 1210
Bst4CI ACNGT 3 cut(s) 13, 502, 625
Bst6I CTCTTC 4 cut(s) 204, 226, 429, 1108
BstACI GRCGYC 1 cut(s) 447
BstAPI GCANNNNNTGC 2 cut(s) 266, 962
BstDEI CTNAG 6 cut(s) 101, 535, 579, 673, 681, 1116
BstDSI CCRYGG 2 cut(s) 564, 903
BstF5I GGATG 5 cut(s) 385, 607, 735, 913, 967
BstH2I RGCGCY 1 cut(s) 450
BstHHI GCGC 2 cut(s) 449, 468
BstKTI GATC 7 cut(s) 206, 324, 522, 765, 774, 927, 978
BstMAI GTCTC 1 cut(s) 976
BstMBI GATC 7 cut(s) 203, 321, 519, 762, 771, 924, 975
BstMCI CGRYCG 1 cut(s) 978
BstMWI GCNNNNNNNGC 7 cut(s) 55, 257, 266, 275, 281, 284, 962
BstNI CCWGG 2 cut(s) 1020, 1210
BstNSI RCATGY 1 cut(s) 660
BstSCI CCNGG 6 cut(s) 948, 949, 991, 1002, 1018, 1208
BstSFI CTRYAG 1 cut(s) 249
BstV1I GCAGC 3 cut(s) 263, 293, 820
BstV2I GAAGAC 1 cut(s) 1065
BsuRI GGCC 5 cut(s) 488, 702, 743, 1084, 1180
BtgI CCRYGG 2 cut(s) 564, 903
BtgZI GCGATG 1 cut(s) 1068
BtsCI GGATG 5 cut(s) 385, 607, 735, 913, 967
BtsI GCAGTG 1 cut(s) 32
BtsIMutI CAGTG 2 cut(s) 32, 460
BveI ACCTGC 1 cut(s) 113
CfoI GCGC 2 cut(s) 449, 468
Cfr13I GGNCC 5 cut(s) 986, 995, 1006, 1082, 1179
Cfr9I CCCGGG 1 cut(s) 949
Csp6I GTAC 3 cut(s) 334, 361, 1190
CviAII CATG 6 cut(s) 208, 337, 437, 565, 657, 931
CviQI GTAC 3 cut(s) 334, 361, 1190
DdeI CTNAG 6 cut(s) 101, 535, 579, 673, 681, 1116
DinI GGCGCC 1 cut(s) 448
DpnI GATC 7 cut(s) 205, 323, 521, 764, 773, 926, 977
DpnII GATC 7 cut(s) 203, 321, 519, 762, 771, 924, 975
DraI TTTAAA 1 cut(s) 714
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 4 cut(s) 204, 226, 429, 1108
EarI CTCTTC 4 cut(s) 204, 226, 429, 1108
Eco130I CCWWGG 1 cut(s) 564
Eco147I AGGCCT 1 cut(s) 702
Eco47I GGWCC 3 cut(s) 986, 995, 1006
Eco88I CYCGRG 1 cut(s) 949
EcoRII CCWGG 2 cut(s) 1018, 1208
EcoT14I CCWWGG 1 cut(s) 564
EgeI GGCGCC 1 cut(s) 448
EheI GGCGCC 1 cut(s) 448
ErhI CCWWGG 1 cut(s) 564
FaeI CATG 6 cut(s) 211, 340, 440, 568, 660, 934
FalI AAGNNNNNCTT 2 cut(s) 758, 790
FaqI GGGAC 2 cut(s) 9, 1008
FatI CATG 6 cut(s) 207, 336, 436, 564, 656, 930
Fnu4HI GCNGC 6 cut(s) 252, 276, 279, 282, 285, 809
FokI GGATG 5 cut(s) 372, 614, 742, 920, 974
Fsp4HI GCNGC 6 cut(s) 252, 276, 279, 282, 285, 809
FspBI CTAG 5 cut(s) 90, 243, 264, 704, 1040
GlaI GCGC 2 cut(s) 448, 467
GluI GCNGC 6 cut(s) 252, 276, 279, 282, 285, 809
GsaI CCCAGC 1 cut(s) 317
HaeII RGCGCY 1 cut(s) 450
HaeIII GGCC 5 cut(s) 488, 702, 743, 1084, 1180
HapII CCGG 3 cut(s) 950, 992, 1004
HhaI GCGC 2 cut(s) 449, 468
Hin1I GRCGYC 1 cut(s) 447
Hin1II CATG 6 cut(s) 211, 340, 440, 568, 660, 934
Hin6I GCGC 2 cut(s) 447, 466
HinP1I GCGC 2 cut(s) 447, 466
HinfI GANTC 5 cut(s) 135, 406, 677, 899, 1219
HpaII CCGG 3 cut(s) 950, 992, 1004
HphI GGTGA 2 cut(s) 19, 137
Hpy166II GTNNAC 4 cut(s) 113, 989, 1068, 1192
Hpy188I TCNGA 4 cut(s) 405, 538, 615, 676
Hpy188III TCNNGA 5 cut(s) 178, 243, 389, 973, 1076
Hpy8I GTNNAC 4 cut(s) 113, 989, 1068, 1192
Hpy99I CGWCG 4 cut(s) 120, 293, 380, 883
HpyAV CCTTC 4 cut(s) 122, 346, 838, 1122
HpyCH4III ACNGT 3 cut(s) 13, 502, 625
HpyCH4IV ACGT 2 cut(s) 375, 841
HpyCH4V TGCA 6 cut(s) 49, 251, 656, 808, 956, 1147
HpyF10VI GCNNNNNNNGC 7 cut(s) 55, 257, 266, 275, 281, 284, 962
HpyF3I CTNAG 6 cut(s) 101, 535, 579, 673, 681, 1116
HpySE526I ACGT 2 cut(s) 375, 841
Hsp92I GRCGYC 1 cut(s) 447
Hsp92II CATG 6 cut(s) 211, 340, 440, 568, 660, 934
HspAI GCGC 2 cut(s) 447, 466
KasI GGCGCC 1 cut(s) 446
Kzo9I GATC 7 cut(s) 203, 321, 519, 762, 771, 924, 975
LmnI GCTCC 2 cut(s) 147, 310
Lsp1109I GCAGC 3 cut(s) 263, 293, 820
LweI GCATC 2 cut(s) 952, 1032
MaeI CTAG 5 cut(s) 90, 243, 264, 704, 1040
MaeII ACGT 2 cut(s) 375, 841
MaeIII GTNAC 2 cut(s) 7, 979
MalI GATC 7 cut(s) 205, 323, 521, 764, 773, 926, 977
MboI GATC 7 cut(s) 203, 321, 519, 762, 771, 924, 975
MlsI TGGCCA 1 cut(s) 488
MluCI AATT 6 cut(s) 41, 305, 493, 663, 715, 914
MluNI TGGCCA 1 cut(s) 488
Mly113I GGCGCC 1 cut(s) 447
MlyI GAGTC 1 cut(s) 144
MmeI TCCRAC 1 cut(s) 282
Mox20I TGGCCA 1 cut(s) 488
MroXI GAANNNNTTC 2 cut(s) 609, 1097
MscI TGGCCA 1 cut(s) 488
MseI TTAA 4 cut(s) 477, 584, 713, 863
Msp20I TGGCCA 1 cut(s) 488
MspI CCGG 3 cut(s) 950, 992, 1004
MspR9I CCNGG 6 cut(s) 950, 951, 993, 1004, 1020, 1210
MvaI CCWGG 2 cut(s) 1020, 1210
MwoI GCNNNNNNNGC 7 cut(s) 55, 257, 266, 275, 281, 284, 962
NarI GGCGCC 1 cut(s) 447
NciI CCSGG 4 cut(s) 950, 951, 993, 1004
NcoI CCATGG 1 cut(s) 564
NdeII GATC 7 cut(s) 203, 321, 519, 762, 771, 924, 975
NlaIII CATG 6 cut(s) 211, 340, 440, 568, 660, 934
NlaIV GGNNCC 5 cut(s) 274, 448, 532, 936, 996
NmuCI GTSAC 2 cut(s) 7, 979
NspI RCATGY 1 cut(s) 660
PaqCI CACCTGC 1 cut(s) 113
PceI AGGCCT 1 cut(s) 702
PdmI GAANNNNTTC 2 cut(s) 609, 1097
PfeI GAWTC 4 cut(s) 406, 677, 899, 1219
PkrI GCNGC 6 cut(s) 253, 277, 280, 283, 286, 810
Ple19I CGATCG 1 cut(s) 978
PleI GAGTC 1 cut(s) 143
PluTI GGCGCC 1 cut(s) 450
PpsI GAGTC 1 cut(s) 143
Psp6I CCWGG 2 cut(s) 1018, 1208
PspFI CCCAGC 1 cut(s) 313
PspGI CCWGG 2 cut(s) 1018, 1208
PspN4I GGNNCC 5 cut(s) 274, 448, 532, 936, 996
PspPI GGNCC 5 cut(s) 986, 995, 1006, 1082, 1179
PstI CTGCAG 1 cut(s) 253
PvuI CGATCG 1 cut(s) 978
RsaI GTAC 3 cut(s) 335, 362, 1191
RsaNI GTAC 3 cut(s) 334, 361, 1190
SaqAI TTAA 4 cut(s) 477, 584, 713, 863
SatI GCNGC 6 cut(s) 252, 276, 279, 282, 285, 809
Sau3AI GATC 7 cut(s) 203, 321, 519, 762, 771, 924, 975
Sau96I GGNCC 5 cut(s) 986, 995, 1006, 1082, 1179
SchI GAGTC 1 cut(s) 144
ScrFI CCNGG 6 cut(s) 950, 951, 993, 1004, 1020, 1210
SfaNI GCATC 2 cut(s) 952, 1032
SfcI CTRYAG 1 cut(s) 249
SfoI GGCGCC 1 cut(s) 448
SinI GGWCC 3 cut(s) 986, 995, 1006
SmaI CCCGGG 1 cut(s) 951
SpeI ACTAGT 2 cut(s) 89, 263
Sse9I AATT 6 cut(s) 41, 305, 493, 663, 715, 914
SseBI AGGCCT 1 cut(s) 702
SsiI CCGC 3 cut(s) 276, 279, 285
SspDI GGCGCC 1 cut(s) 446
SspI AATATT 2 cut(s) 18, 475
SspMI CTAG 5 cut(s) 90, 243, 264, 704, 1040
StuI AGGCCT 1 cut(s) 702
StyD4I CCNGG 6 cut(s) 948, 949, 991, 1002, 1018, 1208
StyI CCWWGG 1 cut(s) 564
TaaI ACNGT 3 cut(s) 13, 502, 625
TaiI ACGT 2 cut(s) 378, 844
TaqI TCGA 4 cut(s) 62, 202, 795, 974
TaqII GACCGA 1 cut(s) 1012
TasI AATT 6 cut(s) 41, 305, 493, 663, 715, 914
TatI WGTACW 1 cut(s) 333
TauI GCSGC 3 cut(s) 278, 281, 287
TfiI GAWTC 4 cut(s) 406, 677, 899, 1219
Tru1I TTAA 4 cut(s) 477, 584, 713, 863
Tru9I TTAA 4 cut(s) 477, 584, 713, 863
TscAI CASTG 2 cut(s) 39, 467
TseFI GTSAC 2 cut(s) 7, 979
TseI GCWGC 3 cut(s) 251, 281, 808
Tsp45I GTSAC 2 cut(s) 7, 979
TspGWI ACGGA 3 cut(s) 134, 892, 1129
TspMI CCCGGG 1 cut(s) 949
TspRI CASTG 2 cut(s) 39, 467
VpaK11BI GGWCC 3 cut(s) 986, 995, 1006
XapI RAATTY 1 cut(s) 41
XbaI TCTAGA 1 cut(s) 242
XceI RCATGY 1 cut(s) 660
XmaI CCCGGG 1 cut(s) 949
XmnI GAANNNNTTC 2 cut(s) 609, 1097
XspI CTAG 5 cut(s) 90, 243, 264, 704, 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.